Lcy10g005130 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy10g005130
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calcium-binding EF-hand family protein
Location	Chr10: 14188468 .. 14189696 (-)
RNA-Seq Expression	Lcy10g005130
Synteny	Lcy10g005130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATGAAAAAAAAAAAAAAAAGTCTAGAAAGGAAGTTTCCGGGAAGTTGCATAGGGTAGTACAAATGTGAGAATTCTCTCCCACCATTTAACCCCCTCAACTTCTTCACACAAATTGCCTCAAATCAAATCAAATCAAACATTCCTCATTTCCTCTCTCTTTCCTTTTCTTCTTCTTCATTGCATTCTCAGATGAAGCTCATCAAAATCAACTCCAAAACCTTCACCCCCAAGCGCCTTTTCGCTTCCAGAAAGGACCGCTCCAAAGTCTCCCGATCCGAACCGCCCTCCTTCGGCTCCGCTTCCTCCGAGGAGTCCTCCACGCCGACCAGCGTCTTGCCGCCGCACCTTCACCTCTCCGGCGACTGGTCCGACTTCTCCGACGGCAGAGTGTCTCGCAAGGAGCTGGAGGCTCTAATGGGCCTGTTCGGCGCTGAGCCACTGAGCGAGGAAGAGCTCAAGATGGTGCTGGGCGAAGTGGACCACGACGGAGACGGCATCGTGAGCGTGGAGGCCCTCATGGGCCGAGTCGGCCCGGCTTGCCACACAGAGCTGCGAGACACGTTCGAGATCTTCGACGCCGACCACGACGGCAGAATCACGGCGGAGGAGCTTTTCAGTGTGTTGTCGACGATTGGGGAGCGGTGCACGTTAGAGGACTGTCGGCGCATGATAGCCGGAGTGGACAAGAACGGGGATGGGTTCGTCTGCTTCGAGGACTTTGCACGTATGATGGACTGTCAGAGATGAATGGAAACCAGACAGACCCCTCTCAATTTTTTTGGTTTAAATTAAATTGTATTAATAAAAAGTAAAATAGGATCTCTGTGGTGAGGGGACTTTTTTTATTAAAATTGTACTTTTTAAAACTTATTACAATAATAGGATTATTTGGAAACTTATTATTACAATATTGTAGTTTTTTTTCCACGTCAATAGGTCGTCTCTTGAAGAGACGACCTGATGACGTGGCACGCCATCTGCCCAATAAATTAAAAAAAAAATAAAAAAATCAGTAGGTCGTCTCTTGAAGAGACGACCTACATTAAAATTTAAAAAAAAAAAAGCGTCAAAAATAGATCGTCTCTTGAAGAGACGATCTATTAACTTGGGTTAACGCAGGTCGTCTCTTTAAGAGACGACCTGCACACATTTTACCATCATTTCATTTATGGCAAGTTGGTGAGTGAGCCACCCAGTCATGTGAGAGGTCCTAGGTTCGAGCTTG

mRNA sequence

AAAAATGAAAAAAAAAAAAAAAAGTCTAGAAAGGAAGTTTCCGGGAAGTTGCATAGGGTAGTACAAATGTGAGAATTCTCTCCCACCATTTAACCCCCTCAACTTCTTCACACAAATTGCCTCAAATCAAATCAAATCAAACATTCCTCATTTCCTCTCTCTTTCCTTTTCTTCTTCTTCATTGCATTCTCAGATGAAGCTCATCAAAATCAACTCCAAAACCTTCACCCCCAAGCGCCTTTTCGCTTCCAGAAAGGACCGCTCCAAAGTCTCCCGATCCGAACCGCCCTCCTTCGGCTCCGCTTCCTCCGAGGAGTCCTCCACGCCGACCAGCGTCTTGCCGCCGCACCTTCACCTCTCCGGCGACTGGTCCGACTTCTCCGACGGCAGAGTGTCTCGCAAGGAGCTGGAGGCTCTAATGGGCCTGTTCGGCGCTGAGCCACTGAGCGAGGAAGAGCTCAAGATGGTGCTGGGCGAAGTGGACCACGACGGAGACGGCATCGTGAGCGTGGAGGCCCTCATGGGCCGAGTCGGCCCGGCTTGCCACACAGAGCTGCGAGACACGTTCGAGATCTTCGACGCCGACCACGACGGCAGAATCACGGCGGAGGAGCTTTTCAGTGTGTTGTCGACGATTGGGGAGCGGTGCACGTTAGAGGACTGTCGGCGCATGATAGCCGGAGTGGACAAGAACGGGGATGGGTTCGTCTGCTTCGAGGACTTTGCACGTATGATGGACTGTCAGAGATGAATGGAAACCAGACAGACCCCTCTCAATTTTTTTGGTTTAAATTAAATTGTATTAATAAAAAGTAAAATAGGATCTCTGTGGTGAGGGGACTTTTTTTATTAAAATTGTACTTTTTAAAACTTATTACAATAATAGGATTATTTGGAAACTTATTATTACAATATTGTAGTTTTTTTTCCACGTCAATAGGTCGTCTCTTGAAGAGACGACCTGATGACGTGGCACGCCATCTGCCCAATAAATTAAAAAAAAAATAAAAAAATCAGTAGGTCGTCTCTTGAAGAGACGACCTACATTAAAATTTAAAAAAAAAAAAGCGTCAAAAATAGATCGTCTCTTGAAGAGACGATCTATTAACTTGGGTTAACGCAGGTCGTCTCTTTAAGAGACGACCTGCACACATTTTACCATCATTTCATTTATGGCAAGTTGGTGAGTGAGCCACCCAGTCATGTGAGAGGTCCTAGGTTCGAGCTTG

Coding sequence (CDS)

ATGAAGCTCATCAAAATCAACTCCAAAACCTTCACCCCCAAGCGCCTTTTCGCTTCCAGAAAGGACCGCTCCAAAGTCTCCCGATCCGAACCGCCCTCCTTCGGCTCCGCTTCCTCCGAGGAGTCCTCCACGCCGACCAGCGTCTTGCCGCCGCACCTTCACCTCTCCGGCGACTGGTCCGACTTCTCCGACGGCAGAGTGTCTCGCAAGGAGCTGGAGGCTCTAATGGGCCTGTTCGGCGCTGAGCCACTGAGCGAGGAAGAGCTCAAGATGGTGCTGGGCGAAGTGGACCACGACGGAGACGGCATCGTGAGCGTGGAGGCCCTCATGGGCCGAGTCGGCCCGGCTTGCCACACAGAGCTGCGAGACACGTTCGAGATCTTCGACGCCGACCACGACGGCAGAATCACGGCGGAGGAGCTTTTCAGTGTGTTGTCGACGATTGGGGAGCGGTGCACGTTAGAGGACTGTCGGCGCATGATAGCCGGAGTGGACAAGAACGGGGATGGGTTCGTCTGCTTCGAGGACTTTGCACGTATGATGGACTGTCAGAGATGA

Protein sequence

MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLPPHLHLSGDWSDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPACHTELRDTFEIFDADHDGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR

Homology

[Close]

BLAST of Lcy10g005130 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.6e-39
Identity = 107/214 (50.00%), Postives = 132/214 (61.68%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPP-SFGSASSEE--------------SSTP 60
           MKL K+      PKR F   KDRS VS+S    SFGSASS                S TP
Sbjct: 1   MKLAKL-----IPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTP 60

Query: 61  TSVLP----PH-----LHLSGDWSDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEV 120
           TS+LP    P+     L         +DG VSR +LE+L+   G +PL+EEE+ ++L EV
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 DHDGDGIVSVEALMGRV---GPA-CHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ER 180
           D DGDG + +E L  RV    PA   TEL++TFE FDAD DG I+A+EL  V STIG ER
Sbjct: 121 DCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

Query: 181 CTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 186
           CTL+DC+RMIA VD++GDGFVCF +F+RMMD QR
Sbjct: 181 CTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lcy10g005130 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 6.2e-33
Identity = 95/201 (47.26%), Postives = 116/201 (57.71%), Query Frame = 0


Query: 12  TPKRLFASRKDRSKVSRSEPPSFGSASSEESS-------------TPTSVLPPHLHLSGD 71
           +PKRLF + K ++ VSRSEP SF S +S  SS             TP SVLP +   SGD
Sbjct: 11  SPKRLFRT-KSKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISVLPQN---SGD 70

Query: 72  W-----------SDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVE 131
           +               DG VSR +L AL+     EP S+EE+ ++L EVD    G +S+E
Sbjct: 71  FYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLE 130

Query: 132 ALMGRV------GPACHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRM 182
            L  RV      G     ELR+ FEIFD D +G+I+AEEL  V   IG ERCTLE+C RM
Sbjct: 131 DLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRM 190

[Close]

BLAST of Lcy10g005130 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.4e-21
Identity = 52/123 (42.28%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 64  DGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALM-----GRVGPACH 123
           DG++S KEL A+M   G E + EEEL+  + E+D  GDG ++ E  +     G       
Sbjct: 50  DGKISSKELGAIMTSLGHE-VPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 124 TELRDTFEIFDADHDGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFEDFA 182
             L+D F ++D D +G I+AEEL  VL ++G+ C++ +CR+MI GVDK+GDG + FE+F 
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFK 169

[Close]

BLAST of Lcy10g005130 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.3e-21
Identity = 67/169 (39.64%), Postives = 90/169 (53.25%), Query Frame = 0


Query: 23  RSKVSRSEP-PSFGSASSEESSTPTSVLPPHLHLSGDWSDF---SDGRVSRKELEALMGL 82
           R   S+S P P    AS   S  PT    P   +   +  F    DGR+SR EL AL   
Sbjct: 12  RRSGSKSPPLPQADPASGGGSPAPT----PEEEMERVFRKFDANGDGRISRSELGALFES 71

Query: 83  FGAEPLSEEELKMVLGEVDHDGDGIVSVE------ALMGRVGPACHTELRDTFEIFDADH 142
            G    +++EL  ++ E D DGDG +S++      A       A   +LR  F +FDAD 
Sbjct: 72  LG-HAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADG 131

Query: 143 DGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFEDFARMM 182
           +G I+A EL  VL  +GE+ T++ CRRMI GVD+NGDG + FE+F  MM
Sbjct: 132 NGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Lcy10g005130 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.6e-20
Identity = 55/124 (44.35%), Postives = 72/124 (58.06%), Query Frame = 0


Query: 64  DGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGP------AC 123
           DGR+SR EL AL    G   ++++E+  ++ E D DGDG +S+        P      A 
Sbjct: 68  DGRISRAELAALFRSVG-HAVTDDEVARMMQEADSDGDGYISLGEFAAISAPPPGDAAAA 127

Query: 124 HTELRDTFEIFDADHDGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFEDF 182
             +LR  F +FDAD +G IT  EL  VL  IGE  T+  CRRMI GVD+NGDG + FE+F
Sbjct: 128 EEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVDRNGDGLINFEEF 187

[Close]

BLAST of Lcy10g005130 vs. ExPASy TrEMBL
Match: A0A6J1J8Y7 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111482360 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.1e-61
Identity = 132/189 (69.84%), Postives = 151/189 (79.89%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLP--PHLHLSGD 60
           MKL KI +K+F+P RLF S+K R+ +S+SEPPSFGSAS    S+PTSVLP  PH HLSG+
Sbjct: 13  MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSAS---FSSPTSVLPPHPHPHLSGE 72

Query: 61  WSDFSDGRVS-RKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPAC 120
           WSDFSD  +  RKELEAL+GL GAEP SEEELKM++ +VD              +VGPAC
Sbjct: 73  WSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVD--------------KVGPAC 132

Query: 121 HTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCFED 180
           H+ELRDTFEIFDADHDGRITAEELFSV S +G E+CTLEDCRRMIAGVDKNGDGFVCFED
Sbjct: 133 HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFED 184

Query: 181 FARMMDCQR 186
           FA MM+CQR
Sbjct: 193 FAVMMECQR 184

[Close]

BLAST of Lcy10g005130 vs. ExPASy TrEMBL
Match: A0A6J1FB49 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111442449 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 3.3e-61
Identity = 131/189 (69.31%), Postives = 150/189 (79.37%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLP--PHLHLSGD 60
           MKL K+ +K+F+P RLF S+K R+ +S+SEPPSFGSAS   SS+PTSVLP  PH HLSG+
Sbjct: 13  MKLPKLTAKSFSPGRLFPSKKHRTSLSKSEPPSFGSAS---SSSPTSVLPPHPHPHLSGE 72

Query: 61  WSDFSDGRVS-RKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPAC 120
           WSDFSD     RKELEAL+GL GAEP SEEELKM++ +VD              +VGP C
Sbjct: 73  WSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVD--------------KVGPPC 132

Query: 121 HTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCFED 180
           H+ELRDTFEIFDADHDGRITAEELFSV S +G E+CTLEDCRRMIAGVDKNGDGFVCFED
Sbjct: 133 HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFED 184

Query: 181 FARMMDCQR 186
           FA MM+CQR
Sbjct: 193 FAVMMECQR 184

[Close]

BLAST of Lcy10g005130 vs. ExPASy TrEMBL
Match: A0A5A7UEZ0 (Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007460 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.8e-59
Identity = 129/191 (67.54%), Postives = 148/191 (77.49%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESS-----TPTSVLPPHLHL 60
           MKL+    K+F P RLF+S+  RSK+SRS+PPSFGSASS  SS     TPTSVLP     
Sbjct: 1   MKLL----KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLP----- 60

Query: 61  SGDWSDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGP 120
           S DW+DFSD  +SRKEL+AL+GL GAEP SEEE+K+++GE+D              RVGP
Sbjct: 61  SLDWADFSDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMD--------------RVGP 120

Query: 121 ACHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCF 180
           ACH+ELRDTFEIFDADHDGRITAEELFSV + +G E CTLEDCRRMIAGVDKNGDGFVCF
Sbjct: 121 ACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCF 168

Query: 181 EDFARMMDCQR 186
           +DFARMMDCQR
Sbjct: 181 DDFARMMDCQR 168

[Close]

BLAST of Lcy10g005130 vs. ExPASy TrEMBL
Match: A0A1S3B7R8 (probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.8e-59
Identity = 129/191 (67.54%), Postives = 148/191 (77.49%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESS-----TPTSVLPPHLHL 60
           MKL+    K+F P RLF+S+  RSK+SRS+PPSFGSASS  SS     TPTSVLP     
Sbjct: 1   MKLL----KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLP----- 60

Query: 61  SGDWSDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGP 120
           S DW+DFSD  +SRKEL+AL+GL GAEP SEEE+K+++GE+D              RVGP
Sbjct: 61  SLDWADFSDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMD--------------RVGP 120

Query: 121 ACHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCF 180
           ACH+ELRDTFEIFDADHDGRITAEELFSV + +G E CTLEDCRRMIAGVDKNGDGFVCF
Sbjct: 121 ACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCF 168

Query: 181 EDFARMMDCQR 186
           +DFARMMDCQR
Sbjct: 181 DDFARMMDCQR 168

[Close]

BLAST of Lcy10g005130 vs. ExPASy TrEMBL
Match: A0A6J1DP84 (probable calcium-binding protein CML35 OS=Momordica charantia OX=3673 GN=LOC111022928 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 8.9e-59
Identity = 131/190 (68.95%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLPPHLHLSGDW- 60
           MKLI    K F+PKRLF S+K+RSKV+RS+PPSF S+SS  ++TPTSVLP  L  + DW 
Sbjct: 1   MKLI----KNFSPKRLFGSKKERSKVTRSDPPSFASSSSSSAATPTSVLPRALS-ADDWP 60

Query: 61  SDFSD-----GRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVG 120
           SDFS       RVSRK+LEAL+GL GA+P S EE+  +L E+D  GDG+VSVEALM  VG
Sbjct: 61  SDFSSDMARHSRVSRKQLEALLGLSGADPPSHEEVAAMLSELDRHGDGVVSVEALMSHVG 120

Query: 121 PACHTELRDTFEIFDADHDGRITAEELFSVLSTIG--ERCTLEDCRRMIAGVDKNGDGFV 180
            AC  +LR+TFEIFDAD DGRITAEELFSV   IG  ERCTLEDCRRMIAGVDKNGDGFV
Sbjct: 121 GACDEDLRETFEIFDADGDGRITAEELFSVFVAIGEEERCTLEDCRRMIAGVDKNGDGFV 180

Query: 181 CFEDFARMMD 183
           CFEDFARMMD
Sbjct: 181 CFEDFARMMD 185

[Close]

BLAST of Lcy10g005130 vs. NCBI nr
Match: XP_022983878.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 246.1 bits (627), Expect = 2.3e-61
Identity = 132/189 (69.84%), Postives = 151/189 (79.89%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLP--PHLHLSGD 60
           MKL KI +K+F+P RLF S+K R+ +S+SEPPSFGSAS    S+PTSVLP  PH HLSG+
Sbjct: 13  MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSAS---FSSPTSVLPPHPHPHLSGE 72

Query: 61  WSDFSDGRVS-RKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPAC 120
           WSDFSD  +  RKELEAL+GL GAEP SEEELKM++ +VD              +VGPAC
Sbjct: 73  WSDFSDTHIDYRKELEALLGLIGAEPPSEEELKMMMSQVD--------------KVGPAC 132

Query: 121 HTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCFED 180
           H+ELRDTFEIFDADHDGRITAEELFSV S +G E+CTLEDCRRMIAGVDKNGDGFVCFED
Sbjct: 133 HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFED 184

Query: 181 FARMMDCQR 186
           FA MM+CQR
Sbjct: 193 FAVMMECQR 184

[Close]

BLAST of Lcy10g005130 vs. NCBI nr
Match: KAG6580931.1 (putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017674.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 245.4 bits (625), Expect = 4.0e-61
Identity = 132/189 (69.84%), Postives = 150/189 (79.37%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLP--PHLHLSGD 60
           MKL KI +K+F+P RLF S+K R+ +S+SEPPSFGSAS   SS+PTSVLP  PH HLSG+
Sbjct: 1   MKLPKITAKSFSPGRLFPSKKHRTSLSKSEPPSFGSAS---SSSPTSVLPPHPHPHLSGE 60

Query: 61  WSDFSDGRVS-RKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPAC 120
           WSDFSD     RKELEAL+GL GAEP SEEELKM++ +VD              +VGP C
Sbjct: 61  WSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVD--------------KVGPPC 120

Query: 121 HTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCFED 180
           H+ELRDTFEIFDADHDGRITAEELFSV S +G E+CTLEDCRRMIAGVDKNGDGFVCFED
Sbjct: 121 HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFED 172

Query: 181 FARMMDCQR 186
           FA MM+CQR
Sbjct: 181 FAVMMECQR 172

[Close]

BLAST of Lcy10g005130 vs. NCBI nr
Match: XP_022935658.1 (probable calcium-binding protein CML36 [Cucurbita moschata])

HSP 1 Score: 244.6 bits (623), Expect = 6.8e-61
Identity = 131/189 (69.31%), Postives = 150/189 (79.37%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLP--PHLHLSGD 60
           MKL K+ +K+F+P RLF S+K R+ +S+SEPPSFGSAS   SS+PTSVLP  PH HLSG+
Sbjct: 13  MKLPKLTAKSFSPGRLFPSKKHRTSLSKSEPPSFGSAS---SSSPTSVLPPHPHPHLSGE 72

Query: 61  WSDFSDGRVS-RKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPAC 120
           WSDFSD     RKELEAL+GL GAEP SEEELKM++ +VD              +VGP C
Sbjct: 73  WSDFSDTHTDYRKELEALLGLIGAEPPSEEELKMMMSQVD--------------KVGPPC 132

Query: 121 HTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCFED 180
           H+ELRDTFEIFDADHDGRITAEELFSV S +G E+CTLEDCRRMIAGVDKNGDGFVCFED
Sbjct: 133 HSELRDTFEIFDADHDGRITAEELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFED 184

Query: 181 FARMMDCQR 186
           FA MM+CQR
Sbjct: 193 FAVMMECQR 184

[Close]

BLAST of Lcy10g005130 vs. NCBI nr
Match: XP_038903370.1 (probable calcium-binding protein CML36 [Benincasa hispida])

HSP 1 Score: 238.8 bits (608), Expect = 3.7e-59
Identity = 130/187 (69.52%), Postives = 146/187 (78.07%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESS-TPTSVLPPHLHLSGDW 60
           MKL+    K+F P RLF+S+  RSK+SRS+PPSFGSASS  S+ TPTSVLP     S  W
Sbjct: 1   MKLL----KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-----SAVW 60

Query: 61  SDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGPACHT 120
           SDFSD  +SRKELEAL+GL GAEP SEEELK ++G +D              RVGPACH+
Sbjct: 61  SDFSDSTLSRKELEALLGLIGAEPPSEEELKTMMGAMD--------------RVGPACHS 120

Query: 121 ELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCFEDFA 180
           ELRDTFEIFDADHDGRITAEELFSV + +G E+CTLEDCRRMIAGVDKNGDGFVCFEDFA
Sbjct: 121 ELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFA 164

Query: 181 RMMDCQR 186
           RMMDCQR
Sbjct: 181 RMMDCQR 164

[Close]

BLAST of Lcy10g005130 vs. NCBI nr
Match: XP_008443339.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa] >TYK25587.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa])

HSP 1 Score: 236.9 bits (603), Expect = 1.4e-58
Identity = 129/191 (67.54%), Postives = 148/191 (77.49%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPPSFGSASSEESS-----TPTSVLPPHLHL 60
           MKL+    K+F P RLF+S+  RSK+SRS+PPSFGSASS  SS     TPTSVLP     
Sbjct: 1   MKLL----KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLP----- 60

Query: 61  SGDWSDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMGRVGP 120
           S DW+DFSD  +SRKEL+AL+GL GAEP SEEE+K+++GE+D              RVGP
Sbjct: 61  SLDWADFSDPTLSRKELKALLGLIGAEPPSEEEIKIMMGEMD--------------RVGP 120

Query: 121 ACHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRMIAGVDKNGDGFVCF 180
           ACH+ELRDTFEIFDADHDGRITAEELFSV + +G E CTLEDCRRMIAGVDKNGDGFVCF
Sbjct: 121 ACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCF 168

Query: 181 EDFARMMDCQR 186
           +DFARMMDCQR
Sbjct: 181 DDFARMMDCQR 168

[Close]

BLAST of Lcy10g005130 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 161.8 bits (408), Expect = 5.4e-40
Identity = 107/214 (50.00%), Postives = 132/214 (61.68%), Query Frame = 0


Query: 1   MKLIKINSKTFTPKRLFASRKDRSKVSRSEPP-SFGSASSEE--------------SSTP 60
           MKL K+      PKR F   KDRS VS+S    SFGSASS                S TP
Sbjct: 1   MKLAKL-----IPKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTP 60

Query: 61  TSVLP----PH-----LHLSGDWSDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEV 120
           TS+LP    P+     L         +DG VSR +LE+L+   G +PL+EEE+ ++L EV
Sbjct: 61  TSILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEV 120

Query: 121 DHDGDGIVSVEALMGRV---GPA-CHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ER 180
           D DGDG + +E L  RV    PA   TEL++TFE FDAD DG I+A+EL  V STIG ER
Sbjct: 121 DCDGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

Query: 181 CTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 186
           CTL+DC+RMIA VD++GDGFVCF +F+RMMD QR
Sbjct: 181 CTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lcy10g005130 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 142.1 bits (357), Expect = 4.4e-34
Identity = 95/201 (47.26%), Postives = 116/201 (57.71%), Query Frame = 0


Query: 12  TPKRLFASRKDRSKVSRSEPPSFGSASSEESS-------------TPTSVLPPHLHLSGD 71
           +PKRLF + K ++ VSRSEP SF S +S  SS             TP SVLP +   SGD
Sbjct: 11  SPKRLFRT-KSKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISVLPQN---SGD 70

Query: 72  W-----------SDFSDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVE 131
           +               DG VSR +L AL+     EP S+EE+ ++L EVD    G +S+E
Sbjct: 71  FYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCISLE 130

Query: 132 ALMGRV------GPACHTELRDTFEIFDADHDGRITAEELFSVLSTIG-ERCTLEDCRRM 182
            L  RV      G     ELR+ FEIFD D +G+I+AEEL  V   IG ERCTLE+C RM
Sbjct: 131 DLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRM 190

[Close]

BLAST of Lcy10g005130 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 102.4 bits (254), Expect = 3.9e-22
Identity = 52/123 (42.28%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 64  DGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALM-----GRVGPACH 123
           DG++S KEL A+M   G E + EEEL+  + E+D  GDG ++ E  +     G       
Sbjct: 50  DGKISSKELGAIMTSLGHE-VPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 124 TELRDTFEIFDADHDGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFEDFA 182
             L+D F ++D D +G I+AEEL  VL ++G+ C++ +CR+MI GVDK+GDG + FE+F 
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFK 169

[Close]

BLAST of Lcy10g005130 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 95.9 bits (237), Expect = 3.6e-20
Identity = 46/126 (36.51%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 63  SDGRVSRKELEALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMG-------RVGP 122
           +DG++S  EL+ ++G       S+EE K ++ E D DG+G + ++  +            
Sbjct: 27  NDGKISIDELKDVIGALSPN-ASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSNN 86

Query: 123 ACHTELRDTFEIFDADHDGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFE 182
           +   +L++ F+++D D +GRI+A EL SV+  +GE+C+++DC+RMI  VD +GDG V FE
Sbjct: 87  SAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKVDSDGDGCVDFE 146

[Close]

BLAST of Lcy10g005130 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.1e-19
Identity = 57/172 (33.14%), Postives = 90/172 (52.33%), Query Frame = 0


Query: 13  PKRLFASRKDRSKVSRSEPPSFGSASSEESSTPTSVLPPHLHLSGDWSDFSDGRVSRKEL 72
           P R+F+  +  S +S   P  F   S  E             +   +    DG++S+ E 
Sbjct: 22  PSRMFSRDRQSSGLSSPGPGGFSQPSVNEMR----------RVFSRFDLDKDGKISQTEY 81

Query: 73  EALMGLFGAEPLSEEELKMVLGEVDHDGDGIVSVEALMG---RVGPACHTELRDTFEIFD 132
           + ++   G E  + E++  +   VD DGDG +     +    R G    +++R++F  FD
Sbjct: 82  KVVLRALGQE-RAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSDIRNSFWTFD 141

Query: 133 ADHDGRITAEELFSVLSTIGERCTLEDCRRMIAGVDKNGDGFVCFEDFARMM 182
            + DG+I+AEE+ SVL  +GERC+LEDC RM+  VD +GDG V  E+F +MM
Sbjct: 142 LNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

The following BLAST results are available for this feature:

BLAST of Lcy10g005130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	7.6e-39	50.00	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	6.2e-33	47.26	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9FYK2	5.4e-21	42.28	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	9.3e-21	39.64	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6L4D4	4.6e-20	44.35	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Lcy10g005130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J8Y7	1.1e-61	69.84	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114823...	[more]
A0A6J1FB49	3.3e-61	69.31	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7UEZ0	6.8e-59	67.54	Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B7R8	6.8e-59	67.54	probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 P...	[more]
A0A6J1DP84	8.9e-59	68.95	probable calcium-binding protein CML35 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Lcy10g005130 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983878.1	2.3e-61	69.84	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
KAG6580931.1	4.0e-61	69.84	putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022935658.1	6.8e-61	69.31	probable calcium-binding protein CML36 [Cucurbita moschata]	[more]
XP_038903370.1	3.7e-59	69.52	probable calcium-binding protein CML36 [Benincasa hispida]	[more]
XP_008443339.2	1.4e-58	67.54	PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 p...	[more]

BLAST of Lcy10g005130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	5.4e-40	50.00	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	4.4e-34	47.26	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	3.9e-22	42.28	EF hand calcium-binding protein family	[more]
AT1G66400.1	3.6e-20	36.51	calmodulin like 23	[more]
AT2G15680.1	3.1e-19	33.14	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 156..184 e-value: 0.0022 score: 27.2 coord: 88..116 e-value: 18.0 score: 7.6 coord: 120..148 e-value: 3.0E-5 score: 33.5
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 64..107 e-value: 7.5E-4 score: 19.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 120..181 e-value: 3.2E-9 score: 37.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 116..151 score: 12.532831
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..185 score: 11.695971
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 120..181 e-value: 8.75563E-20 score: 76.8177
None	No IPR available	GENE3D	1.10.238.10		coord: 115..185 e-value: 7.4E-20 score: 72.9
None	No IPR available	GENE3D	1.10.238.10		coord: 61..113 e-value: 2.2E-8 score: 36.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 15..52
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 29..50
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..183
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 5..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 129..141
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 63..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy10g005130.1	Lcy10g005130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding