Lcy10g003060 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g003060
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING/U-box superfamily protein
LocationChr10: 4904011 .. 4907744 (-)
RNA-Seq ExpressionLcy10g003060
SyntenyLcy10g003060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCATTTCTCAAGCAACAACAATGGCGGACACCATGGATGACGATTACCTTCAAGACATAATCACCGAGCAACGTTCCGAACTCATCGCCGCCAAAACCCTCGTCTCCGACCTCGATTTGGCCTTCCAACTTCAACTGCAGGAGGCCATGAATGCCTCTCTCAAGTCTATGCCCTCGATCGAACCATCTCCGTCACTTAACAGGGCCGCCCCTGTCTTGGAGTCTGAAGAAACCGGCCCTTTGGACTTGGCCACGACTCTTATGCTCGAGGACATTGCGCGCTTCGCCATGGAGTTCAAGGACCGGGAGCGGTGTCAGACTGAGATGAGGAAAATGAAGGAGGAGCTTGACCGTCGGATTCATGATCAGAACTTTGCGAATTACATTCTGAATGTTCCCGAGGATGAGTGGAGGAAGTATGGGGATAATTATGAGAAGCCGTATGGTGAGAGTTCGTCGTCTTCTTCGTCTTCGTCTTCGTCTTCCTCTTCCTCGAGGGGTTTGTTGGATTCTCAGTGCTTCAGGGTGTATTCGAAGGGGTTGATTAGTGAAGAGAGGATTAGAGATATGCAGGTTAGGGTGGCTGGGATTGGCGTTGCGATTTGTGACCCTAAGGATAATCTGTTGTTGGAGAAGATGAAGCCTCTGGAATATGTAGTGGATGGTAAGGAGATCAGCAGTGAATGCGCTGAGCTTGAGGCACTGGTTGAAGGGCTCAATATTGCTCTTGTTTTAGGCTTGACAAATGTCACTTTTTTCTGTGCGGACTACATGCTCTACCAGTATGTAAGTAGCTAGAATTTTTCAGCCACCCCCCCTTTTCATGTCTAGTTTGTTCTATGTTTAGAGTTGAATTGGTTTTTGCTCATTGAATATAGTTCTCTGGTGGAAACAACTAATTAGAAAGCATCATAATATTATTTTAAGAATACTGACCGTATGGGCATGAAGGTTTACTTGATTTAGAATTCATTGTAAACAAGCATTCAATTTGAGAACATAATCAAAATCAAATACTAGTGCGAAGGCTGACCTGTCGTCCTCCCAATCCTTTTTTTTTTTTATGTTCATGAGTGTCCGGACCAGCATGTGCACATTTCTACTAATCTCATGGGACGACCATATGATCCTACTGTATTTGGTTGTCAGAAAATATCTAGGATGTTAAATCCCATATAGGTGGCCAACGGCCATCATGGATTGAACCCATTCCCTTAATCCTAGAGCCTTTGGAGCCTAGAAAAATGACTGGTGATTGTCATTAATCAACTAGTGGCCAATAAGTTCCAAAGTATATAGTAAAAGGCTTAGAGGAAATTGATTCAAGCCATGGTGGCCATCTACGTAAGATTCAATATCTTAGAGTTACCTAAGCAACCAAATATAGTAGGGCTAGATAGTTGTCTTGTGTGAATAGTTGAGGTATGAGGATAGTTGGGGTGTGTGCACATTAGCCCTTGGACAGATACTAAAAGAAAACAAACTGGGGAGTCATAAGACGAGTTCTTAAATAAAGAGCTTTGTTTAGAAGCGAGACAATCTGCAGGGTCCAAATATGCCTAATAAGATAACAAGGTGGGTGTAAACTTTTTCTCTGTTGCATCATCATCTCCACTGTCCTTAGTGGTTAGCTGAATATAATGATCGAGACTAGTTTGAAGTTTTGTGTATTTAGCCGAATGTTAATATGTCATTTATCATTATATGAATTGCCAGGTTTTTCACTCATCATTATTGACAAATAAATGCCTATTGGCTTTTCCTTTGAGTGATTCATCTTTATTGCTATCATAATTATCGTATTATCTGCTTTATTTTCATATAATTGAATGAAGTGCATTAGGAAACTCTAGCACTCGGTATTACTGGTGACCTTCTCATTTCCATATCAAATATGGAAAAGTAGTATAAGAAAGAATTGTGATTTAGTTCATCCATATTCTTTGGTTACAGCTTACAGGTAGGGTGCCTCCAGGAGCGAGCTCACTGGCAACATTGGTTAATGAGGTGGTTCTTCTTCAGGGAAAATTTACCTTTTGTAACCCGTCCCTCGTGACACGTAATGATATCAAGTTTGCTTTTAGACTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCCAGCAGAAGCTGACAATGGAAAGTGCTTGAAAGAAACTTGTACAATCTGCTTTGAAGATGTTCGTGCAGATCAGATGTTCTCAATTGATGGTTGTTTGCACAGATACTGCTTTTCTTGTATGAAGCAGCATGTTGAAGTAAGGTTGCTCAATGGGGATGGGATGGTGGTAAATTGTCCTCATCAGGGTTGTAAGTCAGAAATTAATATTGAAAGCTGTGGAAAGTTCTTGGAAAGTAAGGTGATCGAAATTATGAGCCAAAGGATTAAAGAAGCGTCTGTGCCTGTTCTGGAGAAAGTTTACTGCCCCTATTCGAAGTGCTCGGCATTAATGTCTAAAAGCGAGCTTTTAAAATATACGCAGTCTTCTTATATTGATGCAGAACGATCGGGGGCAAGGAAGTGCATGAAATGTAATCTGTTTTTCTGTATTAATTGCAAGGTCCCTTGGCACTATAATCTGACTTGCTATGACTACAGAAAGTTAAATCCTAATCCCCGTCCAGATGAGGCAATGCTGAAGTCCCTTGCATCAAGGAAACTATGGCGCCAATGCATTAAGTGTAACAATATGGTTGAATTGGCCGAAGGCTGCTACCACATCACCTGCAGGTGCATTATTTACCCTTATCTATTTCGCTTGCTCCTTTTCTCCTTTGATGAACATTATTCCTTCATATATGTAGCTGCTAATCAAGCAGAAAAGTGTAACCGGTTAAAAAATGCAATATTTCTTAAGCACTTAATGAGCATGAATGCAATCCACCAATGCCTACTTGGATGGAAGATGGAGCAAGGGTAATAGTAACTAAAATAGAGAAATTCTCTTAAGAGTTATTATAAAATGAATACTGAAAAATACCCCTCCAAGTTGGTTGACTGCACTCTACTTTGGCGATTCTGCTTTACTGGATAATTGGTAGTGATTTGTCTTGTCAAAGAAAATAATAACCATGTAATGCTTACTACAAGTTTCTTTGTGAAAGTGCACATTGGAGTTTTCTTTCCCTGCTTCTAAATGTCATTGGATTCTGTCTGCAGATGTGGATATGAGTTCTGCTATACATGTGGAGCCCCCTGGAAAAAGAAGAAGGCTACATGTTCCTGTCCAATTTGGGACGAGCGTAACATCATCCGTGATCAACGTCGAAGATGAGCTTTTTAGCTGAGAATTTGGGGGATGACCGCCCCTTTCTTTCCCTATTCAGTTTCTGTTTCTTTGCACTCTTTTGGAAGGTAGCCCAAAGTCAGACACCAGATATTTTGTGCAGTTCTCTAGTTGGTGACATACTGATATTAGATCACAGCTTTTTATTATCTTTAAATGTCTCGTTTTCAAACTTGCAAGACATAGTACATAAGGTAGTAGGTTTAAGGGAACCGATGGAAGAAAACAGGTGTAGAGAGCAAGTTTCTTCCCGACTTTTGACATGAAGGGTTTTCATGTACAGCTTTCTTAATCCACAAATTTCTTTCGTGCTTAGATAGGTATGTTCAAAGAATTAGGAGAATTTTGGAAACATTCTGTTGGCTAAGCATTAGAATAATGATGCTTGTTATTCTAGCAACCAGAGATTCCATGTCAATATTCTTGTTAAAGCATTTTCATGATTGT

mRNA sequence

GCATTTCTCAAGCAACAACAATGGCGGACACCATGGATGACGATTACCTTCAAGACATAATCACCGAGCAACGTTCCGAACTCATCGCCGCCAAAACCCTCGTCTCCGACCTCGATTTGGCCTTCCAACTTCAACTGCAGGAGGCCATGAATGCCTCTCTCAAGTCTATGCCCTCGATCGAACCATCTCCGTCACTTAACAGGGCCGCCCCTGTCTTGGAGTCTGAAGAAACCGGCCCTTTGGACTTGGCCACGACTCTTATGCTCGAGGACATTGCGCGCTTCGCCATGGAGTTCAAGGACCGGGAGCGGTGTCAGACTGAGATGAGGAAAATGAAGGAGGAGCTTGACCGTCGGATTCATGATCAGAACTTTGCGAATTACATTCTGAATGTTCCCGAGGATGAGTGGAGGAAGTATGGGGATAATTATGAGAAGCCGTATGGTGAGAGTTCGTCGTCTTCTTCGTCTTCGTCTTCGTCTTCCTCTTCCTCGAGGGGTTTGTTGGATTCTCAGTGCTTCAGGGTGTATTCGAAGGGGTTGATTAGTGAAGAGAGGATTAGAGATATGCAGGTTAGGGTGGCTGGGATTGGCGTTGCGATTTGTGACCCTAAGGATAATCTGTTGTTGGAGAAGATGAAGCCTCTGGAATATGTAGTGGATGGTAAGGAGATCAGCAGTGAATGCGCTGAGCTTGAGGCACTGGTTGAAGGGCTCAATATTGCTCTTGTTTTAGGCTTGACAAATGTCACTTTTTTCTGTGCGGACTACATGCTCTACCAGTATCTTACAGGTAGGGTGCCTCCAGGAGCGAGCTCACTGGCAACATTGGTTAATGAGGTGGTTCTTCTTCAGGGAAAATTTACCTTTTGTAACCCGTCCCTCGTGACACGTAATGATATCAAGTTTGCTTTTAGACTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCCAGCAGAAGCTGACAATGGAAAGTGCTTGAAAGAAACTTGTACAATCTGCTTTGAAGATGTTCGTGCAGATCAGATGTTCTCAATTGATGGTTGTTTGCACAGATACTGCTTTTCTTGTATGAAGCAGCATGTTGAAGTAAGGTTGCTCAATGGGGATGGGATGGTGGTAAATTGTCCTCATCAGGGTTGTAAGTCAGAAATTAATATTGAAAGCTGTGGAAAGTTCTTGGAAAGTAAGGTGATCGAAATTATGAGCCAAAGGATTAAAGAAGCGTCTGTGCCTGTTCTGGAGAAAGTTTACTGCCCCTATTCGAAGTGCTCGGCATTAATGTCTAAAAGCGAGCTTTTAAAATATACGCAGTCTTCTTATATTGATGCAGAACGATCGGGGGCAAGGAAGTGCATGAAATGTAATCTGTTTTTCTGTATTAATTGCAAGGTCCCTTGGCACTATAATCTGACTTGCTATGACTACAGAAAGTTAAATCCTAATCCCCGTCCAGATGAGGCAATGCTGAAGTCCCTTGCATCAAGGAAACTATGGCGCCAATGCATTAAGTGTAACAATATGGTTGAATTGGCCGAAGGCTGCTACCACATCACCTGCAGATGTGGATATGAGTTCTGCTATACATGTGGAGCCCCCTGGAAAAAGAAGAAGGCTACATGTTCCTGTCCAATTTGGGACGAGCGTAACATCATCCGTGATCAACGTCGAAGATGAGCTTTTTAGCTGAGAATTTGGGGGATGACCGCCCCTTTCTTTCCCTATTCAGTTTCTGTTTCTTTGCACTCTTTTGGAAGGTAGCCCAAAGTCAGACACCAGATATTTTGTGCAGTTCTCTAGTTGGTGACATACTGATATTAGATCACAGCTTTTTATTATCTTTAAATGTCTCGTTTTCAAACTTGCAAGACATAGTACATAAGGTAGTAGGTTTAAGGGAACCGATGGAAGAAAACAGGTGTAGAGAGCAAGTTTCTTCCCGACTTTTGACATGAAGGGTTTTCATGTACAGCTTTCTTAATCCACAAATTTCTTTCGTGCTTAGATAGGTATGTTCAAAGAATTAGGAGAATTTTGGAAACATTCTGTTGGCTAAGCATTAGAATAATGATGCTTGTTATTCTAGCAACCAGAGATTCCATGTCAATATTCTTGTTAAAGCATTTTCATGATTGT

Coding sequence (CDS)

ATGGCGGACACCATGGATGACGATTACCTTCAAGACATAATCACCGAGCAACGTTCCGAACTCATCGCCGCCAAAACCCTCGTCTCCGACCTCGATTTGGCCTTCCAACTTCAACTGCAGGAGGCCATGAATGCCTCTCTCAAGTCTATGCCCTCGATCGAACCATCTCCGTCACTTAACAGGGCCGCCCCTGTCTTGGAGTCTGAAGAAACCGGCCCTTTGGACTTGGCCACGACTCTTATGCTCGAGGACATTGCGCGCTTCGCCATGGAGTTCAAGGACCGGGAGCGGTGTCAGACTGAGATGAGGAAAATGAAGGAGGAGCTTGACCGTCGGATTCATGATCAGAACTTTGCGAATTACATTCTGAATGTTCCCGAGGATGAGTGGAGGAAGTATGGGGATAATTATGAGAAGCCGTATGGTGAGAGTTCGTCGTCTTCTTCGTCTTCGTCTTCGTCTTCCTCTTCCTCGAGGGGTTTGTTGGATTCTCAGTGCTTCAGGGTGTATTCGAAGGGGTTGATTAGTGAAGAGAGGATTAGAGATATGCAGGTTAGGGTGGCTGGGATTGGCGTTGCGATTTGTGACCCTAAGGATAATCTGTTGTTGGAGAAGATGAAGCCTCTGGAATATGTAGTGGATGGTAAGGAGATCAGCAGTGAATGCGCTGAGCTTGAGGCACTGGTTGAAGGGCTCAATATTGCTCTTGTTTTAGGCTTGACAAATGTCACTTTTTTCTGTGCGGACTACATGCTCTACCAGTATCTTACAGGTAGGGTGCCTCCAGGAGCGAGCTCACTGGCAACATTGGTTAATGAGGTGGTTCTTCTTCAGGGAAAATTTACCTTTTGTAACCCGTCCCTCGTGACACGTAATGATATCAAGTTTGCTTTTAGACTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCCAGCAGAAGCTGACAATGGAAAGTGCTTGAAAGAAACTTGTACAATCTGCTTTGAAGATGTTCGTGCAGATCAGATGTTCTCAATTGATGGTTGTTTGCACAGATACTGCTTTTCTTGTATGAAGCAGCATGTTGAAGTAAGGTTGCTCAATGGGGATGGGATGGTGGTAAATTGTCCTCATCAGGGTTGTAAGTCAGAAATTAATATTGAAAGCTGTGGAAAGTTCTTGGAAAGTAAGGTGATCGAAATTATGAGCCAAAGGATTAAAGAAGCGTCTGTGCCTGTTCTGGAGAAAGTTTACTGCCCCTATTCGAAGTGCTCGGCATTAATGTCTAAAAGCGAGCTTTTAAAATATACGCAGTCTTCTTATATTGATGCAGAACGATCGGGGGCAAGGAAGTGCATGAAATGTAATCTGTTTTTCTGTATTAATTGCAAGGTCCCTTGGCACTATAATCTGACTTGCTATGACTACAGAAAGTTAAATCCTAATCCCCGTCCAGATGAGGCAATGCTGAAGTCCCTTGCATCAAGGAAACTATGGCGCCAATGCATTAAGTGTAACAATATGGTTGAATTGGCCGAAGGCTGCTACCACATCACCTGCAGATGTGGATATGAGTTCTGCTATACATGTGGAGCCCCCTGGAAAAAGAAGAAGGCTACATGTTCCTGTCCAATTTGGGACGAGCGTAACATCATCCGTGATCAACGTCGAAGATGA

Protein sequence

MADTMDDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSLNRAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFANYILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSCPIWDERNIIRDQRRR
Homology
BLAST of Lcy10g003060 vs. ExPASy Swiss-Prot
Match: P0CE10 (ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01020 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.9e-17
Identity = 54/202 (26.73%), Postives = 89/202 (44.06%), Query Frame = 0

Query: 325  CTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESC 384
            C IC  +V  D  +S++GC H +C +C+ +  E  + N D   + C H  C + I +   
Sbjct: 1561 CPICLSEV--DDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1620

Query: 385  GKFL-ESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQSSYIDAERSG 444
               L + K+ E++S  +         K+ +C    C ++   +             E   
Sbjct: 1621 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIYRVAG----------PQESGE 1680

Query: 445  ARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNM 504
               C  C+   C  C + +H  +TC  Y+K   NP   +  LK  A  K  ++C  C + 
Sbjct: 1681 PFICGACHSETCTRCHLEYHPLITCERYKKFKENP---DLSLKDWAKGKDVKECPICKST 1740

Query: 505  VELAEGCYHITCRCGYEFCYTC 525
            +E  +GC H+ CRCG   C+TC
Sbjct: 1741 IEKTDGCNHLQCRCGKHICWTC 1747

BLAST of Lcy10g003060 vs. ExPASy Swiss-Prot
Match: F4KGU4 (ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10370 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.9e-17
Identity = 54/202 (26.73%), Postives = 87/202 (43.07%), Query Frame = 0

Query: 325  CTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESC 384
            C IC  +V  D  +S++GC H +C +C+ +  E  + N D   + C H  C + I +   
Sbjct: 1564 CPICLSEV--DDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVLADM 1623

Query: 385  GKFL-ESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQSSYIDAERSG 444
               L + K+ E+ S  +         K  +C    C ++   +             E   
Sbjct: 1624 RALLSQEKLDELFSASLSSFVTSSDGKFRFCSTPDCPSVYRVAG----------PQESGE 1683

Query: 445  ARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNM 504
               C  C+   C  C + +H  +TC  Y+K   NP   +  LK  A  K  ++C  C + 
Sbjct: 1684 PFICGACHSEICTRCHLEYHPLITCERYKKFKENP---DLSLKDWAKGKNVKECPICKST 1743

Query: 505  VELAEGCYHITCRCGYEFCYTC 525
            +E  +GC H+ CRCG   C+TC
Sbjct: 1744 IEKTDGCNHMKCRCGKHICWTC 1750

BLAST of Lcy10g003060 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.4e-16
Identity = 63/222 (28.38%), Postives = 99/222 (44.59%), Query Frame = 0

Query: 324 TCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVN-CPHQGCKSEINIE 383
           +C IC ED    Q F++  C HRYC  C K ++E+++  G   +   CP   CK  ++ +
Sbjct: 138 SCLICLEDYPPTQTFALI-CNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQD 197

Query: 384 SCGKFLESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQSSYIDAERS 443
           +  + +  +V E  +  I ++ V    +V +CP   C   +                ER 
Sbjct: 198 AFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGCIYSIRCDR-----------KERK 257

Query: 444 GARKCMKCNLFFCINC---KVPWHYNLTCYDYRK-LNPNPRPDEAMLKSLASRKLWRQCI 503
            A  C KC   +C NC   ++  H    C    K L       E +   LA+ K   +C 
Sbjct: 258 EAVNC-KCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCP 317

Query: 504 KCNNMVELAEGCYHITCR-----CGYEFCYTCGAPWKKKKAT 535
           +C + +E   GC H+TCR     CG+EFC+ C  PW +  +T
Sbjct: 318 ECRSPIEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGST 343

BLAST of Lcy10g003060 vs. ExPASy Swiss-Prot
Match: Q9US46 (E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=itt1 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.2e-12
Identity = 59/245 (24.08%), Postives = 98/245 (40.00%), Query Frame = 0

Query: 322 KETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINI 381
           K  C +CF++      F +  C H  C SC++ +  + +  G    + C    C  +  +
Sbjct: 171 KFQCNVCFDEFNGTDCFQLTRCGHVSCQSCLRDYYTMCIQEGMFSQIKCIDLDCGKDAPV 230

Query: 382 ESCGKFLESKVIEIMSQRIKEASVPVLEK-----VYCPYSKCSALMSKSELLKYTQSSYI 441
            +  K LES V   ++ R KE       +     ++CP S C                  
Sbjct: 231 LTL-KELESIVGVQLTNRYKELEEKRRYENDSNIIFCPRSFCQG---------------- 290

Query: 442 DAERSGARK---CMKCNLFFCINCKVPWHYNLT-CY---DYRKL------NPNPRPDEAM 501
            ++R   +K   C KC+  FC  C+  WH +L+ C    D +KL           P++A+
Sbjct: 291 PSKRDPGQKLAICQKCDFAFCSFCQATWHGDLSPCKLEGDSKKLVEMYLNYQENEPEKAL 350

Query: 502 -----------------LKSLASRKLW-----RQCIKCNNMVELAEGCYHITCRCGYEFC 527
                            +K+    + W     ++C  C+ +VE  +GC H+ C CG  FC
Sbjct: 351 ELEKRYGKRIIDRLVEQVKNDEEAEKWVLLNGQRCPTCDRVVERIDGCCHMNCLCGTHFC 398

BLAST of Lcy10g003060 vs. ExPASy Swiss-Prot
Match: Q9LVW9 (Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI4 PE=5 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 2.4e-11
Identity = 50/202 (24.75%), Postives = 86/202 (42.57%), Query Frame = 0

Query: 325 CTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESC 384
           C IC E+  +    +   C HR+C  C K+H  VR+  G+G  + C    C +    +  
Sbjct: 119 CDICMEEDLSKYAMTRMECGHRFCNDCWKEHFTVRINEGEGKRIRCMAYKCNT--ICDEA 178

Query: 385 GKFLESKVIEIMSQRIKEASVPVLEKV-YCPYSKCSALMSKSELLKYTQSSYIDAERSGA 444
            + + +++ E   + + E+ V     V +CP    S     + +        +D      
Sbjct: 179 RQLVSTELAEKFDRFLIESYVEDNNMVKWCP----STPHCGNAIRNIKDDGDVDEVE--- 238

Query: 445 RKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNMV 504
                C L FC +C    H   +C  + KL      DE+   +  +    + C KC+  +
Sbjct: 239 ---CSCGLQFCFSCLSESHSPCSCLMW-KLWKKKCEDESETVNWMTVNT-KLCPKCSKPI 298

Query: 505 ELAEGCYHITCRCGYEFCYTCG 526
           +  +GC H+TC+CG  FC+ CG
Sbjct: 299 QKRDGCNHMTCKCGQHFCWLCG 306

BLAST of Lcy10g003060 vs. ExPASy TrEMBL
Match: A0A6J1DZJ9 (uncharacterized protein LOC111024540 OS=Momordica charantia OX=3673 GN=LOC111024540 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.9e-278
Identity = 476/555 (85.77%), Postives = 510/555 (91.89%), Query Frame = 0

Query: 1   MADTM-DDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADTM DDDYLQ++++EQRSEL+AAKTL SDLDLAFQLQLQEAMNASL S PS +PSPS 
Sbjct: 1   MADTMDDDDYLQEVVSEQRSELLAAKTLFSDLDLAFQLQLQEAMNASLDSKPSTQPSPSR 60

Query: 61  NRAAPVLESEE-TGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNF 120
           NR  P LESE+ +  LDLATTLMLEDIARFAMEFKDRE+C TEMRKMKEELDRRIHDQNF
Sbjct: 61  NRVVPKLESEDSSSALDLATTLMLEDIARFAMEFKDREQCHTEMRKMKEELDRRIHDQNF 120

Query: 121 ANYILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEE 180
           A YI NVPEDEWR+YGDNYEKPY   SS SSSSSSSSS +R  LDS+ FRVYSKGLISEE
Sbjct: 121 ATYIRNVPEDEWRRYGDNYEKPY-SGSSGSSSSSSSSSPARVCLDSEIFRVYSKGLISEE 180

Query: 181 RIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVL 240
           RIRDM+VRVA IGVA+CDPKDNLL EKM PLE VVDGKEI+SECAELEALVEGLN+AL+L
Sbjct: 181 RIRDMKVRVAAIGVAVCDPKDNLLFEKMMPLESVVDGKEINSECAELEALVEGLNVALIL 240

Query: 241 GLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAF 300
           GL NVTFFCADYMLYQYLTGRVPPGASSLATLVNEV+LLQ +FT+CNPSLVTRND+KFAF
Sbjct: 241 GLKNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVILLQRRFTYCNPSLVTRNDMKFAF 300

Query: 301 RLAREAIVSQITWPAEADNGKCL-KETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           +LAREAIVSQITWP EA NGKCL KETC ICFEDVR DQMFS+DGCLHRYCFSCMKQHVE
Sbjct: 301 KLAREAIVSQITWPTEAGNGKCLKKETCIICFEDVRVDQMFSVDGCLHRYCFSCMKQHVE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           VRLLNG+GM VNCPHQGC S INIESCGKFLE KVIEIMSQRIKEASVP+LEKVYCPYS+
Sbjct: 361 VRLLNGNGMKVNCPHQGCTSGINIESCGKFLEPKVIEIMSQRIKEASVPILEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKYT++S+I AE+SGARKCMKCN FFCINCKVPWHYNLTCYDYRK NPNP
Sbjct: 421 CSALMSKSELLKYTEASFIGAEQSGARKCMKCNQFFCINCKVPWHYNLTCYDYRKSNPNP 480

Query: 481 RPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSC 540
           RPDE MLKSLASRKLWRQC+KCNNMVELAEGCYHITCRCGYEFCY CGAPWK KK TC+C
Sbjct: 481 RPDETMLKSLASRKLWRQCVKCNNMVELAEGCYHITCRCGYEFCYICGAPWKNKKGTCTC 540

Query: 541 PIWDERNIIRDQRRR 553
           PIWDERNIIRDQR++
Sbjct: 541 PIWDERNIIRDQRQQ 554

BLAST of Lcy10g003060 vs. ExPASy TrEMBL
Match: A0A6J1HMI1 (uncharacterized protein LOC111464930 OS=Cucurbita moschata OX=3662 GN=LOC111464930 PE=3 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.3e-274
Identity = 464/555 (83.60%), Postives = 507/555 (91.35%), Query Frame = 0

Query: 1   MADTMDDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDDYLQD+ITEQRSE++AAKTL+SDLDLA+QLQ+QEAMNASL S  S +   ++N
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKGPATVN 60

Query: 61  RAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEELDRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSS---SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSSSSSSS   SSRGLLDS+CFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISE 180

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGL+IALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 240

Query: 241 LGLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQY+   V PG+SS+ATLVNEV LLQGKFT+CNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VIEI+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY+++SY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 481 RPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSC 540
           RPDE MLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC+C
Sbjct: 481 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 541 PIWDERNIIRDQRRR 553
           PIWDERNIIR+QR+R
Sbjct: 541 PIWDERNIIRNQRQR 555

BLAST of Lcy10g003060 vs. ExPASy TrEMBL
Match: A0A5A7U9A4 (Putative E3 ubiquitin-protein ligase rbrA OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold769G00460 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.1e-269
Identity = 454/547 (83.00%), Postives = 495/547 (90.49%), Query Frame = 0

Query: 1   MADTM-DDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADT+ DDD +Q++IT+QRSEL++AKTL SDLD AFQLQLQEAM+ASL S+PSIE S SL
Sbjct: 1   MADTLNDDDCVQELITQQRSELLSAKTLFSDLDYAFQLQLQEAMDASLTSIPSIESSSSL 60

Query: 61  NRAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFA 120
           N A PVL+SEET  LDLATTLMLEDIARFAMEFKDRE CQTEMRKMKEELDRRIHDQ FA
Sbjct: 61  NTADPVLDSEETSGLDLATTLMLEDIARFAMEFKDREHCQTEMRKMKEELDRRIHDQKFA 120

Query: 121 NYILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEER 180
            YI +VPE+EWR+YGDNYEKPYGESSSSSSSSSS +      +DS+CFRVYSKGLISEER
Sbjct: 121 EYIRSVPENEWREYGDNYEKPYGESSSSSSSSSSWN------VDSECFRVYSKGLISEER 180

Query: 181 IRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLG 240
           IRD  VRVAGIG+A+CDPKDNLL EKM P+E +V+GKE S+ECAELEALVEGLN+AL+LG
Sbjct: 181 IRDTVVRVAGIGIAVCDPKDNLLFEKMSPIESMVEGKETSNECAELEALVEGLNVALILG 240

Query: 241 LTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFR 300
           L ++TFFCADYMLYQYLTGRVPP  SS A LVNEVV+LQGKFT+CNPSLVTRNDIKFAF+
Sbjct: 241 LKSITFFCADYMLYQYLTGRVPPATSSTAKLVNEVVVLQGKFTYCNPSLVTRNDIKFAFK 300

Query: 301 LAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVR 360
           LAREAIVSQITWPAEADNG CLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQHVEV+
Sbjct: 301 LAREAIVSQITWPAEADNGNCLKETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHVEVK 360

Query: 361 LLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCS 420
           LLNG+GM  NCPHQGC SE+NIESCGKFLE KV EIMSQRIKEASVP+ EKVYCPYS+CS
Sbjct: 361 LLNGNGMQANCPHQGCTSEVNIESCGKFLEPKVFEIMSQRIKEASVPIQEKVYCPYSRCS 420

Query: 421 ALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRP 480
           ALMSK+ELL+YT++SYIDAER+GARKCMKCN FFCINCKV WHYNLTCYDYR LNPN  P
Sbjct: 421 ALMSKTELLRYTEASYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDYRNLNPNLHP 480

Query: 481 DEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSCPI 540
           DE MLKSLA+RKLWRQCI CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC CPI
Sbjct: 481 DEQMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCKCPI 540

Query: 541 WDERNII 547
           WDERNII
Sbjct: 541 WDERNII 541

BLAST of Lcy10g003060 vs. ExPASy TrEMBL
Match: A0A1S3CJH8 (uncharacterized protein LOC103501665 OS=Cucumis melo OX=3656 GN=LOC103501665 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.1e-269
Identity = 454/547 (83.00%), Postives = 495/547 (90.49%), Query Frame = 0

Query: 1   MADTM-DDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADT+ DDD +Q++IT+QRSEL++AKTL SDLD AFQLQLQEAM+ASL S+PSIE S SL
Sbjct: 1   MADTLNDDDCVQELITQQRSELLSAKTLFSDLDYAFQLQLQEAMDASLTSIPSIESSSSL 60

Query: 61  NRAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFA 120
           N A PVL+SEET  LDLATTLMLEDIARFAMEFKDRE CQTEMRKMKEELDRRIHDQ FA
Sbjct: 61  NTADPVLDSEETSGLDLATTLMLEDIARFAMEFKDREHCQTEMRKMKEELDRRIHDQKFA 120

Query: 121 NYILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEER 180
            YI +VPE+EWR+YGDNYEKPYGESSSSSSSSSS +      +DS+CFRVYSKGLISEER
Sbjct: 121 EYIRSVPENEWREYGDNYEKPYGESSSSSSSSSSWN------VDSECFRVYSKGLISEER 180

Query: 181 IRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLG 240
           IRD  VRVAGIG+A+CDPKDNLL EKM P+E +V+GKE S+ECAELEALVEGLN+AL+LG
Sbjct: 181 IRDTVVRVAGIGIAVCDPKDNLLFEKMSPIESMVEGKETSNECAELEALVEGLNVALILG 240

Query: 241 LTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFR 300
           L ++TFFCADYMLYQYLTGRVPP  SS A LVNEVV+LQGKFT+CNPSLVTRNDIKFAF+
Sbjct: 241 LKSITFFCADYMLYQYLTGRVPPATSSTAKLVNEVVVLQGKFTYCNPSLVTRNDIKFAFK 300

Query: 301 LAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVR 360
           LAREAIVSQITWPAEADNG CLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQHVEV+
Sbjct: 301 LAREAIVSQITWPAEADNGNCLKETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHVEVK 360

Query: 361 LLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCS 420
           LLNG+GM  NCPHQGC SE+NIESCGKFLE KV EIMSQRIKEASVP+ EKVYCPYS+CS
Sbjct: 361 LLNGNGMQANCPHQGCTSEVNIESCGKFLEPKVFEIMSQRIKEASVPIQEKVYCPYSRCS 420

Query: 421 ALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRP 480
           ALMSK+ELL+YT++SYIDAER+GARKCMKCN FFCINCKV WHYNLTCYDYR LNPN  P
Sbjct: 421 ALMSKTELLRYTEASYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDYRNLNPNLHP 480

Query: 481 DEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSCPI 540
           DE MLKSLA+RKLWRQCI CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC CPI
Sbjct: 481 DEQMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCKCPI 540

Query: 541 WDERNII 547
           WDERNII
Sbjct: 541 WDERNII 541

BLAST of Lcy10g003060 vs. ExPASy TrEMBL
Match: A0A0A0LXM8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G088460 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.3e-266
Identity = 454/548 (82.85%), Postives = 494/548 (90.15%), Query Frame = 0

Query: 1   MADTM-DDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADTM DDD +QD+IT+QRSE+++AKTL SDLD AFQLQLQEAM+ASL S PS + S SL
Sbjct: 1   MADTMNDDDCVQDLITQQRSEILSAKTLFSDLDFAFQLQLQEAMDASLASKPSTDSSSSL 60

Query: 61  NRAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFA 120
           N   PVL+SE+T  LDLATTLMLEDIARFAMEFKDRE+CQTEMRKMKEELDRRIHDQ FA
Sbjct: 61  NTPDPVLDSEDTSGLDLATTLMLEDIARFAMEFKDREQCQTEMRKMKEELDRRIHDQKFA 120

Query: 121 NYILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEER 180
            +I +VPE+EWR+YGDNYEKPYGESSSSSSSSSSSS S    +DS+C RVYSKGLISEER
Sbjct: 121 EFIRSVPENEWREYGDNYEKPYGESSSSSSSSSSSSWS----VDSECCRVYSKGLISEER 180

Query: 181 IRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLG 240
           IRDM VRVAGIGVA+CDPKDNLL EKM P+E +V+GKE SSECAELEALVEGLN+AL+LG
Sbjct: 181 IRDMVVRVAGIGVAVCDPKDNLLFEKMSPIESMVEGKETSSECAELEALVEGLNVALILG 240

Query: 241 LTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFR 300
           L  VTFFCADYMLYQYLTGRVPP  SS   LVNEVV+LQGKFT+CNPSLVTRNDIKFAF+
Sbjct: 241 LKTVTFFCADYMLYQYLTGRVPPATSSTEKLVNEVVVLQGKFTYCNPSLVTRNDIKFAFK 300

Query: 301 LAREAIVSQITWPAEADNGKCLK-ETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEV 360
           LAREAIVSQITWPA+A+NGKCLK ETCTICFEDV  DQMFS+DGCLHRYCFSCMKQHVEV
Sbjct: 301 LAREAIVSQITWPAQAENGKCLKEETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHVEV 360

Query: 361 RLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKC 420
           RLLNG+GM  NCPHQGC SE+NIESCGKFLE KV EIMSQRIKEASVPV EKVYCPYS+C
Sbjct: 361 RLLNGNGMQANCPHQGCTSELNIESCGKFLEPKVFEIMSQRIKEASVPVQEKVYCPYSRC 420

Query: 421 SALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPR 480
           SALMSK++LL+YT++ YIDAER+GARKCMKCN FFCINCKV WHYNLTCYD+RKLNPN  
Sbjct: 421 SALMSKTDLLRYTEAFYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDHRKLNPNLH 480

Query: 481 PDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSCP 540
           PDE MLKSLA+RKLWRQCI CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC CP
Sbjct: 481 PDEKMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCKCP 540

Query: 541 IWDERNII 547
           IWDERNII
Sbjct: 541 IWDERNII 544

BLAST of Lcy10g003060 vs. NCBI nr
Match: XP_022157931.1 (uncharacterized protein LOC111024540 [Momordica charantia])

HSP 1 Score: 967.2 bits (2499), Expect = 5.9e-278
Identity = 476/555 (85.77%), Postives = 510/555 (91.89%), Query Frame = 0

Query: 1   MADTM-DDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSL 60
           MADTM DDDYLQ++++EQRSEL+AAKTL SDLDLAFQLQLQEAMNASL S PS +PSPS 
Sbjct: 1   MADTMDDDDYLQEVVSEQRSELLAAKTLFSDLDLAFQLQLQEAMNASLDSKPSTQPSPSR 60

Query: 61  NRAAPVLESEE-TGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNF 120
           NR  P LESE+ +  LDLATTLMLEDIARFAMEFKDRE+C TEMRKMKEELDRRIHDQNF
Sbjct: 61  NRVVPKLESEDSSSALDLATTLMLEDIARFAMEFKDREQCHTEMRKMKEELDRRIHDQNF 120

Query: 121 ANYILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEE 180
           A YI NVPEDEWR+YGDNYEKPY   SS SSSSSSSSS +R  LDS+ FRVYSKGLISEE
Sbjct: 121 ATYIRNVPEDEWRRYGDNYEKPY-SGSSGSSSSSSSSSPARVCLDSEIFRVYSKGLISEE 180

Query: 181 RIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVL 240
           RIRDM+VRVA IGVA+CDPKDNLL EKM PLE VVDGKEI+SECAELEALVEGLN+AL+L
Sbjct: 181 RIRDMKVRVAAIGVAVCDPKDNLLFEKMMPLESVVDGKEINSECAELEALVEGLNVALIL 240

Query: 241 GLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAF 300
           GL NVTFFCADYMLYQYLTGRVPPGASSLATLVNEV+LLQ +FT+CNPSLVTRND+KFAF
Sbjct: 241 GLKNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVILLQRRFTYCNPSLVTRNDMKFAF 300

Query: 301 RLAREAIVSQITWPAEADNGKCL-KETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           +LAREAIVSQITWP EA NGKCL KETC ICFEDVR DQMFS+DGCLHRYCFSCMKQHVE
Sbjct: 301 KLAREAIVSQITWPTEAGNGKCLKKETCIICFEDVRVDQMFSVDGCLHRYCFSCMKQHVE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           VRLLNG+GM VNCPHQGC S INIESCGKFLE KVIEIMSQRIKEASVP+LEKVYCPYS+
Sbjct: 361 VRLLNGNGMKVNCPHQGCTSGINIESCGKFLEPKVIEIMSQRIKEASVPILEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKYT++S+I AE+SGARKCMKCN FFCINCKVPWHYNLTCYDYRK NPNP
Sbjct: 421 CSALMSKSELLKYTEASFIGAEQSGARKCMKCNQFFCINCKVPWHYNLTCYDYRKSNPNP 480

Query: 481 RPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSC 540
           RPDE MLKSLASRKLWRQC+KCNNMVELAEGCYHITCRCGYEFCY CGAPWK KK TC+C
Sbjct: 481 RPDETMLKSLASRKLWRQCVKCNNMVELAEGCYHITCRCGYEFCYICGAPWKNKKGTCTC 540

Query: 541 PIWDERNIIRDQRRR 553
           PIWDERNIIRDQR++
Sbjct: 541 PIWDERNIIRDQRQQ 554

BLAST of Lcy10g003060 vs. NCBI nr
Match: XP_038893965.1 (uncharacterized protein LOC120082751 [Benincasa hispida])

HSP 1 Score: 964.9 bits (2493), Expect = 2.9e-277
Identity = 470/552 (85.14%), Postives = 502/552 (90.94%), Query Frame = 0

Query: 1   MADTMDDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADTMDDD +QD+IT+QRSEL++AKTL SDLD AFQLQLQEAMNASL S PS EPS SLN
Sbjct: 1   MADTMDDDSIQDLITQQRSELLSAKTLFSDLDFAFQLQLQEAMNASLASKPSTEPSSSLN 60

Query: 61  RAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
            A PVL+SEETG LDLATTLMLEDIARFAMEFKDRE+C  EMRKMKEELDRR+HDQ FA 
Sbjct: 61  TAVPVLDSEETGALDLATTLMLEDIARFAMEFKDREQCHNEMRKMKEELDRRVHDQKFAE 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSSSSRGLLDSQCFRVYSKGLISEERI 180
           YI NVPE+EWRKYGDNYEKPYGESSSSSSS SS SS     +DS+CFRVYSKGLISEERI
Sbjct: 121 YIRNVPENEWRKYGDNYEKPYGESSSSSSSCSSPSS-----VDSECFRVYSKGLISEERI 180

Query: 181 RDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGL 240
           RDM VRVAGIGVA+CDPKDNLL EK  PLE +VD KE SS+CAELEALVEGLN+AL+LGL
Sbjct: 181 RDMVVRVAGIGVAVCDPKDNLLFEKTSPLESMVDSKETSSQCAELEALVEGLNVALILGL 240

Query: 241 TNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFRL 300
            NVTFFC DYMLYQYLTGRV PGASS+A LVNEVVLLQGKFT+CNPSLVTRNDIKFAFRL
Sbjct: 241 RNVTFFCGDYMLYQYLTGRVLPGASSMAKLVNEVVLLQGKFTYCNPSLVTRNDIKFAFRL 300

Query: 301 AREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRL 360
           AREAIVSQITWPAEA+NGKCLKETCTICFEDVR DQMF +DGCLHRYCFSCMKQHVEVRL
Sbjct: 301 AREAIVSQITWPAEAENGKCLKETCTICFEDVRVDQMFLVDGCLHRYCFSCMKQHVEVRL 360

Query: 361 LNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSA 420
           LNG+GM  NCPHQGC SEINIESCGKFLE KV EIMSQRIKEASVP+LEKVYCPYS+CS 
Sbjct: 361 LNGNGMQANCPHQGCASEINIESCGKFLEPKVFEIMSQRIKEASVPLLEKVYCPYSRCSE 420

Query: 421 LMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPD 480
           LMSK+ELL+YT+ SYIDA+RSGARKCMKCN FFCINCKV WHYNLTCYD+RKLNPN R D
Sbjct: 421 LMSKTELLRYTEDSYIDAKRSGARKCMKCNRFFCINCKVAWHYNLTCYDFRKLNPNLRSD 480

Query: 481 EAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSCPIW 540
           E MLKSLAS+KLWRQC+ CNNMVELAEGCYHITCRCGYEFCYTCGAPWK KK TC+CPIW
Sbjct: 481 EKMLKSLASQKLWRQCVMCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCNCPIW 540

Query: 541 DERNIIRDQRRR 553
           DERNIIR+QRRR
Sbjct: 541 DERNIIRNQRRR 547

BLAST of Lcy10g003060 vs. NCBI nr
Match: KAG7019256.1 (dbl4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 953.7 bits (2464), Expect = 6.7e-274
Identity = 464/555 (83.60%), Postives = 507/555 (91.35%), Query Frame = 0

Query: 1   MADTMDDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDDYLQD+ITEQRSE++AAKTL+SDLDLA+QLQ+QEAMNASL S  S +   ++N
Sbjct: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63

Query: 61  RAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEELDRRIHDQNFAN
Sbjct: 64  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 123

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSS---SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSSSSSSS   SSRGLLDS+CFRVYSKGLISE
Sbjct: 124 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISE 183

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGL+IALV
Sbjct: 184 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 243

Query: 241 LGLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQY+   V PG+SS+ATLVNEV LLQGKFT+CNPSLVTRNDIKFA
Sbjct: 244 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 303

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 304 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 363

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VIEI+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 364 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 423

Query: 421 CSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY+++SY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 424 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 483

Query: 481 RPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSC 540
           RPDE MLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC+C
Sbjct: 484 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 543

Query: 541 PIWDERNIIRDQRRR 553
           PIWDERNIIR+QR+R
Sbjct: 544 PIWDERNIIRNQRQR 558

BLAST of Lcy10g003060 vs. NCBI nr
Match: XP_022964983.1 (uncharacterized protein LOC111464930 [Cucurbita moschata] >KAG6583501.1 hypothetical protein SDJN03_19433, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 953.7 bits (2464), Expect = 6.7e-274
Identity = 464/555 (83.60%), Postives = 507/555 (91.35%), Query Frame = 0

Query: 1   MADTMDDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDDYLQD+ITEQRSE++AAKTL+SDLDLA+QLQ+QEAMNASL S  S +   ++N
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKGPATVN 60

Query: 61  RAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEELDRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSS---SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSSSSSSS   SSRGLLDS+CFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISE 180

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGL+IALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 240

Query: 241 LGLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQY+   V PG+SS+ATLVNEV LLQGKFT+CNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFEDV  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VIEI+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY+++SY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 481 RPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSC 540
           RPDE MLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC+C
Sbjct: 481 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 541 PIWDERNIIRDQRRR 553
           PIWDERNIIR+QR+R
Sbjct: 541 PIWDERNIIRNQRQR 555

BLAST of Lcy10g003060 vs. NCBI nr
Match: XP_023519052.1 (uncharacterized protein LOC111782521 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950.3 bits (2455), Expect = 7.5e-273
Identity = 460/555 (82.88%), Postives = 507/555 (91.35%), Query Frame = 0

Query: 1   MADTMDDDYLQDIITEQRSELIAAKTLVSDLDLAFQLQLQEAMNASLKSMPSIEPSPSLN 60
           MADT+DDDYLQD+ITEQRSE++AAKTL+SDLDLA+QLQ+QEAM+ASL S  S +   S+N
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMDASLGSKSSSKRPASVN 60

Query: 61  RAAPVLESEETGPLDLATTLMLEDIARFAMEFKDRERCQTEMRKMKEELDRRIHDQNFAN 120
           R  PVLESEETG LD ATTL+LEDIAR AME KDRE+CQ EMRKMKEE+DRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEEIDRRIHDQNFAN 120

Query: 121 YILNVPEDEWRKYGDNYEKPYGESSSSSSSSSSSSS---SSRGLLDSQCFRVYSKGLISE 180
           YILNVP+DEW KYGDNYEKPYGES SSSSSSSSSSS   S+RGLLDS+CFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASTRGLLDSECFRVYSKGLISE 180

Query: 181 ERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALV 240
           ERIRDM VRVAGIGVA+CDPKDNLL EK +PLE VVDGKE S ECAELEALVEGLNIALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLNIALV 240

Query: 241 LGLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFA 300
           LGL  VTFFCADYMLYQY+   V PG+SS+AT+VNEV LLQGKFT+CNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATVVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 301 FRLAREAIVSQITWPAEADNGKCLKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVE 360
           FRLAREAIVSQITWPAE++NGKCLKETCTICFED+  DQMFS+DGCLHRYCFSCMKQ +E
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDICVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 361 VRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSK 420
           +RLLNG+GM  NCPH GC SEINIESCGKFL+S+VI+I+SQRIKEAS+PVLEKVYCPYS+
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIDIISQRIKEASMPVLEKVYCPYSR 420

Query: 421 CSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNP 480
           CSALMSKSELLKY+++SY DAER+GARKCMKCNLFFCINCK PWHYN+TCYD+RKLNP  
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 481 RPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATCSC 540
           RPDE MLKSLAS+KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KK TC+C
Sbjct: 481 RPDEEMLKSLASKKLWRQCVACNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 541 PIWDERNIIRDQRRR 553
           PIWDERNIIR+QR+R
Sbjct: 541 PIWDERNIIRNQRQR 555

BLAST of Lcy10g003060 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 387.1 bits (993), Expect = 2.3e-107
Identity = 221/535 (41.31%), Postives = 318/535 (59.44%), Query Frame = 0

Query: 30  DLDLAFQLQLQEAMNASLKSM---PSIEPSPSLNRAAPVLESEETGPLDLATTLMLEDIA 89
           D + AF+LQ++EA+ ASL S    P   PSP +     VL   E  P D  T    +  +
Sbjct: 4   DSEYAFRLQMEEALAASLSSQSRTPQRPPSPPIVATCGVL-IVENNPNDSKTA---KTSS 63

Query: 90  RFAMEFKDRERCQTEMRKMK--EELDRRIHDQNFANYILNVPEDEWRKYGDNYEKPYG-- 149
            +  +FK          K K  E +     D+   N  L         +G+    P    
Sbjct: 64  GYGFDFKRAVGGGNSKAKGKTHESVTGVRTDERNPNIGLGNSRSTSTGHGNTKPLPQETV 123

Query: 150 ESSSSSSSSSSSSSSSRGLLDSQ-CFRVYSKGLISEERIR-DMQVRVAGIGVAICDPKDN 209
             +      SS  +++ G ++    +R+Y KGL+S+E  +  M   V+G GVAICD +DN
Sbjct: 124 RPAQLVGEGSSRVNTAAGKINGNLLYRLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDN 183

Query: 210 LLLEKMKPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYLTGRV 269
           LL E   PL   +D   +S + AEL+AL+ GL  AL LG+ ++ FFC  Y ++QY+TG+ 
Sbjct: 184 LLFEMKGPL---ID-NGMSRQGAELKALIRGLTEALKLGIKHIVFFCDSYPIFQYVTGKW 243

Query: 270 PPGASSLATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKC 329
                 ++ L++++  +   F+     LV RND+KFA++LARE+I+S +T   +    K 
Sbjct: 244 MAKQKKISLLLDDLQSIMQHFSSYQHVLVARNDVKFAYKLARESILSMVTPHEDPRQAKA 303

Query: 330 -LKETCTICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEI 389
            LKE C ICF D+ A+ MFS+D C HR+CF C+KQHVEV+LL+  GM   CPH GCKSE+
Sbjct: 304 VLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLH--GMAPKCPHDGCKSEL 363

Query: 390 NIESCGKFLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQSSYIDAE 449
            I++CGK L  K+ ++  QR++E ++PV E+VYCPY +CSALMSK+++ +  +S      
Sbjct: 364 VIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYP 423

Query: 450 RSGARKCMKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKC 509
           +SG R+C++C   FC++CKVPWH NL+C +Y+KL+P P  D+  LKSLA+ K+WRQC KC
Sbjct: 424 KSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPADDVKLKSLANNKMWRQCGKC 483

Query: 510 NNMVELAEGCYHITCRCGYEFCYTCGAPWKKKKATC--SCPIWDERNIIRDQRRR 553
            +M+EL++GC HITCRCG+EFCY CG  W K   TC   CP W+E  I R    R
Sbjct: 484 QHMIELSQGCNHITCRCGHEFCYNCGGGWNKIMGTCLNRCPTWNEEYITRQDPAR 528

BLAST of Lcy10g003060 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 1.3e-86
Identity = 172/385 (44.68%), Postives = 236/385 (61.30%), Query Frame = 0

Query: 149 SSSSSSSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRV--AGIGVAICDPKDNLLLEKM 208
           +S    SS +   +D+  +R+  KGL++ E + D   +   AG GVAICD KDNLL E  
Sbjct: 136 NSKGEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK 195

Query: 209 KPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYLTGRVPPGASS 268
           + L  +    EIS    E+ ALV GL+ +  LG+ NV  +C D  +YQ + GR       
Sbjct: 196 ESLRDI----EISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGR-GKSKKK 255

Query: 269 LATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCT 328
           +  LV EV  +  K    +  LV RND+KFAFRLAREAI  + +    A+ G    ETC 
Sbjct: 256 IDHLVEEVQGILEKMACIDAVLVARNDVKFAFRLAREAI-GRNSVDVNAEQG----ETCG 315

Query: 329 ICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGK 388
           I FE+   + MF  + CLHR+CF C+KQHV+V+L +  G    C   GCK ++ +E C K
Sbjct: 316 IFFEETDVEHMFVTEKCLHRHCFPCVKQHVKVKLRS--GTEPTCLEYGCKFKLTLERCSK 375

Query: 389 FLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQSSYIDAERSGARKC 448
            L  K+IE+  Q++KE S+P  E++YCPY  CS LMSK+EL         +A+ S  R C
Sbjct: 376 VLTLKLIEMWKQKMKEDSIPAAERIYCPYPNCSMLMSKTELSS-------EADLSNVRTC 435

Query: 449 MKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNMVELA 508
           +KC   FCI+CKVP H +L+  DY+KL+P+P  D+  LKSLA+ K+WRQC+KC +M+EL+
Sbjct: 436 VKCCGLFCIDCKVPSHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELS 495

Query: 509 EGCYHITCRCGYEFCYTCGAPWKKK 532
            GC H+TCRCGYEFCY CG  W+K+
Sbjct: 496 HGCNHMTCRCGYEFCYECGIEWQKR 501

BLAST of Lcy10g003060 vs. TAIR 10
Match: AT3G45580.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 300.8 bits (769), Expect = 2.2e-81
Identity = 160/371 (43.13%), Postives = 227/371 (61.19%), Query Frame = 0

Query: 167 FRVYSKGLISEERIRDMQVRVAGIGVAICDPKDNLLLEKMKPLEYVVDGKEISSECAELE 226
           +R+Y KGL+SEE +      +AG GVAICD  DNLL + MK  E V D +    E  E+ 
Sbjct: 28  YRLYFKGLVSEETVE----LLAGFGVAICDKDDNLLFQ-MK--EQVHDSRVTVLE-VEIM 87

Query: 227 ALVEGLNIALVLGLTNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVVLLQGKFTFCNP 286
           AL  GL  A+ LG+ N++ +   Y +++ +  +      + A L++ V  ++ + T   P
Sbjct: 88  ALKRGLTEAVGLGIDNISIYSDHYRIFELVMEKSASAEENFALLMDNVQHIRQRLTSSFP 147

Query: 287 SLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCTICFED-VRADQMFSIDGCLH 346
            LVTRN IKF + LA E IVS+I+     D+ K    TC+IC +D    + MFS+  C H
Sbjct: 148 VLVTRNQIKFVYELAMETIVSEISIHI-PDHDK----TCSICSDDNFEPELMFSVALCGH 207

Query: 347 RYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGKFLESKVIEIMSQRIKEASV 406
            +C  C+K+H+EVRLL G   V  C H  C+S++ + +C   L SK+  +   RI+E S+
Sbjct: 208 EFCVECVKRHIEVRLLAGG--VPRCLHYQCESKLTLANCANLLTSKLKAMWELRIEEESI 267

Query: 407 PVLEKVYCPYSKCSALMSKSELLKYTQSSYIDAERSGARKCMKCNLFFCINCKVPWHYNL 466
           PV E+VYCP  +CS+LMS ++L   T+      E    R C+KC   FCINCK+PWH NL
Sbjct: 268 PVEERVYCPNPRCSSLMSVTKLSNSTR------EDVTMRSCVKCGEPFCINCKLPWHSNL 327

Query: 467 TCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNMVELAEGCYHITCRCGYEFCYTCG 526
           +C DY+ L PNP  D+  LK+LA++K+WRQC  C N++EL+EGC HITCRCG++FCY CG
Sbjct: 328 SCNDYKSLGPNPTADDIKLKALANQKMWRQCENCKNVIELSEGCMHITCRCGHQFCYKCG 377

Query: 527 APWKKKKATCS 537
           A W   +  C+
Sbjct: 388 AKWITGRVFCT 377

BLAST of Lcy10g003060 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 4.9e-81
Identity = 167/397 (42.07%), Postives = 235/397 (59.19%), Query Frame = 0

Query: 148 SSSSSSSSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRV-AGIGVAICDPKDNLLLEKM 207
           SS    S+  ++  +D+  +++Y KGL+S E   D +  V  G GVAICD  DNL     
Sbjct: 17  SSKVGQSTFETQSCVDNVVYKLYFKGLVSGETATDKKAIVKVGFGVAICDEADNLFYGMN 76

Query: 208 KPLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYLTGRVPPGASS 267
           K L   V    I+ E A++ AL+ GL  ++  G+ NV   C D  +YQ + GR  P   +
Sbjct: 77  KSLYEAV----INREEADILALITGLYESIHRGVKNVVICCDDRQIYQTIIGREKPQQKN 136

Query: 268 LATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCT 327
           +  L+ EV  L+G+       LV   D  FA RLA +A+V       +A   K L  TC+
Sbjct: 137 V-HLLEEVQRLRGRLASTGTVLVATRDDNFALRLAIDALV-------KATQEKPL--TCS 196

Query: 328 ICFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGK 387
           IC +   A+ M   D CLHR+CFSC+KQ V+V+L +  G+V  C   GCKSE+ +ESC  
Sbjct: 197 ICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRS--GIVPPCLEDGCKSELTLESCSM 256

Query: 388 FLESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQSSYIDAERSGARKC 447
            L  K+IE+  ++++E  +P  EK+YCPY  CS LMSK+EL +       +AE+S  R C
Sbjct: 257 VLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTELSR-------EAEQSNVRAC 316

Query: 448 MKCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNMVELA 507
           +KC+  FCI+CKVPWH +L+C DY++++     ++ MLK LA+ ++WRQC +C +M+EL 
Sbjct: 317 IKCSELFCIDCKVPWHSDLSCADYKRIHSERLVNDMMLKVLANDQMWRQCSECKHMIELT 376

Query: 508 EGCYHITCRCGYEFCYTCGAPWKKKKATCSCPIWDER 544
           EGC HITCRCGYEFCY CG  W K     SC + D R
Sbjct: 377 EGCNHITCRCGYEFCYRCGHKWTKYH-HYSCQLMDNR 389

BLAST of Lcy10g003060 vs. TAIR 10
Match: AT2G21420.1 (IBR domain containing protein )

HSP 1 Score: 295.0 bits (754), Expect = 1.2e-79
Identity = 166/384 (43.23%), Postives = 226/384 (58.85%), Query Frame = 0

Query: 149 SSSSSSSSSSRGLLDSQCFRVYSKGLISEERIRDMQVRV-AGIGVAICDPKDNLLLEKMK 208
           S  + S+S+S    DS  +R++ KGL+S+E   DM+  V AG G+AICD  + LL    K
Sbjct: 32  SMVAESASASNIFADSLVYRLFFKGLVSDETTTDMEEIVKAGFGIAICDEANTLLYNMKK 91

Query: 209 PLEYVVDGKEISSECAELEALVEGLNIALVLGLTNVTFFCADYMLYQYLTGRVPPGASSL 268
            L        I+ E  E++AL+  LN+++ + L NV   C DY ++Q LTGR  P   ++
Sbjct: 92  SLN---GDDVINPEEVEIKALICVLNVSIQMELRNVMICCGDYQIFQILTGRGKP-QQNI 151

Query: 269 ATLVNEVVLLQGKFTFCNPSLVTRNDIKFAFRLAREAIVSQITWPAEADNGKCLKETCTI 328
             LV +V  L+GK +     LV R D+     LA EAI                 ETC I
Sbjct: 152 VHLVEQVQHLRGKLSSTEVVLVPRADV---IILAIEAIGG---------------ETCCI 211

Query: 329 CFEDVRADQMFSIDGCLHRYCFSCMKQHVEVRLLNGDGMVVNCPHQGCKSEINIESCGKF 388
           C E+  AD+MF  + C HR CFSC+ +HV+  LL   G+   C H  C SE+  ESC K 
Sbjct: 212 CRENTDADRMFFTENCFHRQCFSCVNRHVQRMLL--CGISPTCLHFPCNSELTFESCSKV 271

Query: 389 LESKVIEIMSQRIKEASVPVLEKVYCPYSKCSALMSKSELLKYTQSSYIDAERSGARKCM 448
           L   +IE   ++I+E  VP  +K+YCPY +CS LMSK+ L + T       ++S  R C+
Sbjct: 272 LTPNLIEFWKRKIEEDLVPAADKIYCPYRRCSMLMSKTALSRET-------DQSNVRACI 331

Query: 449 KCNLFFCINCKVPWHYNLTCYDYRKLNPNPRPDEAMLKSLASRKLWRQCIKCNNMVELAE 508
           KC   FCI+CKVP H  L+C DY+KLNP+P  D   LKSLA++K WRQC++C+N+VEL E
Sbjct: 332 KCCRLFCIDCKVPSHAGLSCVDYKKLNPDPLYD-VKLKSLANKKKWRQCVQCSNLVELFE 383

Query: 509 GCYHITCRCGYEFCYTCGAPWKKK 532
           GC HITCRCG+EFCY CG  W ++
Sbjct: 392 GCNHITCRCGFEFCYVCGKEWNQR 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0CE102.9e-1726.73ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
F4KGU44.9e-1726.73ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q6T4862.4e-1628.38Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
Q9US466.2e-1224.08E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9LVW92.4e-1124.75Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Match NameE-valueIdentityDescription
A0A6J1DZJ92.9e-27885.77uncharacterized protein LOC111024540 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1HMI13.3e-27483.60uncharacterized protein LOC111464930 OS=Cucurbita moschata OX=3662 GN=LOC1114649... [more]
A0A5A7U9A44.1e-26983.00Putative E3 ubiquitin-protein ligase rbrA OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CJH84.1e-26983.00uncharacterized protein LOC103501665 OS=Cucumis melo OX=3656 GN=LOC103501665 PE=... [more]
A0A0A0LXM87.3e-26682.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G088460 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022157931.15.9e-27885.77uncharacterized protein LOC111024540 [Momordica charantia][more]
XP_038893965.12.9e-27785.14uncharacterized protein LOC120082751 [Benincasa hispida][more]
KAG7019256.16.7e-27483.60dbl4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022964983.16.7e-27483.60uncharacterized protein LOC111464930 [Cucurbita moschata] >KAG6583501.1 hypothet... [more]
XP_023519052.17.5e-27382.88uncharacterized protein LOC111782521 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G60250.12.3e-10741.31zinc finger (C3HC4-type RING finger) family protein [more]
AT2G25370.11.3e-8644.68RING/U-box superfamily protein [more]
AT3G45580.12.2e-8143.13RING/U-box protein with C6HC-type zinc finger [more]
AT5G37560.14.9e-8142.07RING/U-box superfamily protein [more]
AT2G21420.11.2e-7943.23IBR domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 394..467
e-value: 5.1E-14
score: 62.6
coord: 477..537
e-value: 0.0057
score: 25.9
IPR002867IBR domainPFAMPF01485IBRcoord: 396..467
e-value: 1.2E-12
score: 47.9
coord: 483..535
e-value: 4.5E-8
score: 33.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 186..304
e-value: 4.6E-17
score: 62.0
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 325..371
e-value: 8.6E-5
score: 22.3
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 319..405
e-value: 2.3E-16
score: 61.5
NoneNo IPR availableGENE3D1.20.120.1750coord: 465..552
e-value: 1.4E-16
score: 63.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..160
NoneNo IPR availablePANTHERPTHR11685:SF252RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 40..545
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 324..406
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 482..544
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 176..310
e-value: 4.3E-7
score: 31.9
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 40..545
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 343..352
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 325..371
score: 9.376395
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 321..541
score: 24.056988

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g003060.1Lcy10g003060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity