Lcy10g002450 (gene) Sponge gourd (P93075) v1

Overview
NameLcy10g002450
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionExocyst subunit Exo70 family protein
LocationChr10: 3400242 .. 3402701 (-)
RNA-Seq ExpressionLcy10g002450
SyntenyLcy10g002450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCATATGTCTCCGTTTTTGACTAATCCGAATCGTTTTGGTCAAATAAGACAAGAAGCAGCAACGGCAGGCAATTGACCAAACAAACAAATAAATAAATAAATAAAAGCGTTTATTAAAACGACCGCGTAACTCTGAGTTGACTCGTTCGAGTTAAGAAACAAATAAACTCAGTCAATCTCCATTCTGCTGCTTCGAACCTTCCTCACCTTTTCAGTCTCTCTATAAATTCCTCTTTGCCGCCATAGCCATTACCAAACTCAAGCTTTTCTTTCTCCTTTTCCAAAGATGAAAAACCATTTCTTCAAGTCTCTTACGCCTTCGCATTCTCCTGCCCGATCGACGCCGTCTCATTCCCCTGCCCGAACGACGCCGTCGCATGGCTTCTGCGACTCTCTCATGGAGGAGAACATCGAAGTTGCAGAAACCCTAATTACGAAGTGGGACTATGCTGCTTCCTCTTACGCCGATATCACTCCTCTCTTCCAAGACGATCGCTACGAGGCCAAGCAGTACATCAAGGCTGTGAAAGATTTGCAGACGGCGATGCAGCATTTCGGCTTCGAGCATTCCAACTCTCAACATCTTGTTCGCGCTCAGAATCTGATGCAGACCGCTATGAAGAGGTTGCAGAGGGAGTTTCACCGGATTCTGACTGAGAATCGGATACATCTGGATCCGGAATCGATTTCGAATCGGTCGTCTAGGGATTCGGTTTTCACTGGTAACTCTGATGTGGAGGATGAGTCTGAGGACGATTTGCGATTTGCGAACGAGAATGTTCCTGAGGAGGAGCGGATGTCCAGATCGGCTATAGCGGATTTGAAATTGATTGCGGAAGGTATGATTTCTGTTGGTTATGGTAAAGAGTGTGGGAAGATTTACACCGTTAGTCGGAAATCGATTGTTGAGGAAGGTTTGTACAATCTCGGAGTTGCGAAGACGAGTTACCATCATGTTCATAGGATGGAGTGGGAGGTTTTGGAGGTGAAGATCAAGAACTGGCTGAACGCTGTGAAAATTGCTGTTAAAACTTTCTTCGAAGCAGAGAGATTCCTCTGCGATCAAGTTTTTTCGATTTCCGCTTCGATTAGAGAGTCTGTTTTCTCAGAAATTACCAAAGAGAGCGCTCTGACTCTGTTTGGCTTCCCGGAAATGGCGGTGAAATCGAAGAAAACTCCCGAGAAAATTTTCCTGATTCTCGATCTGTACGAAGCGATTTTCGACCTTTTGCCGGAGATTGAATCGATGTTCTCCTGCGAATCGACGTCCTCCGTCCGATCTGTAATCGACCATTCGCTAACAAAAATCGCCGAAAGCATCCGCTCCATGCTCGTCGATTTCGAAGCTCACATCCACAAAGATTCCTCCAAAACGCCAGTGCCGAGCGGCGGAATTCATCCGCTCACTCGCTACGTGATGAACTACATCGCATTCCTCAGCGATTACAGCGGAATCCTCCCCGGAATAGTCGCCGACTGGCCGTTGACGCTTCATTCTCCATTGCCGGAATCCTTCTTCGGCGGCAACGATTCGGAAGAAAATCCTCTCACCATACGCTTGGCCTGGATGATTCTGGTCCTCCTCTCGAAGCTCGACAGCAAAGCGGAGCTCTACCACGACGCGCCTCTGTCGTACATCTTCTTGGCAAACAACCTCGAGTACATCGTCGTGAAGGTACGAACCTCGAATCTCAGATTCCTTTTAGGAGACGAGTGGATCGAGAGCCACGAGGCGAAGGTGAAGCAGTACGCATCGAGCTACCAACGAATGGGATGGAGCAGAGTGTTCCTATCGCTGCCGGAGAATCCGACGGCGGAGATCTCACCAGAGAGAGCAAGAAAACACTTCCACGATTTCAACATGGCATTCGAAGAAGCCTACAGGCATCAAGCCTCGTGGATCGTGACGGATCCGAAGCTGCGCGAGCACATCAAGATCTCGTTGGGGAAGAAACTTGGCACGCTCTACGGTGAGTTTTACATCAAATACCGTTCGCGATTGGAGAATCTTTATGGATTGGATTCGGAGGTGAGATTTGCGCCTGATGATTTGGGAAACTATTTGTCTGATTTGCTTCATGGGGATGGAAATTTGGGAGGGAGTGTTTCTTCATCGTCGTCGTCGTCGACGTCGCCGTATCATTTCCACGGTGGTCGGCGGAGTGGATAAGGCCGGCGGTGGAAGAGGAGGTTTTAGGATAAGGACAAATTTAAGAAGTGTCGCTGGATATAAATTTTCAGACGTGTAAAAAATCAAATTGAGTCTGTTTTTTCTTTTTGATTGATTGATTAATTTGTATGGATTGGAATGGAATGGACGTAATGTAAATTCTCTTATTAGATGTTAACTTATTACTTTAGGACACATGTTAGTGGAGTTGGGTATGTGGACCTTTTTTTGTGAAATCATTATCTTCGTGATTTCTTTTCTTTGTTTTTTTTTTAAAAAAGAGTTATC

mRNA sequence

ATCCATATGTCTCCGTTTTTGACTAATCCGAATCGTTTTGGTCAAATAAGACAAGAAGCAGCAACGGCAGGCAATTGACCAAACAAACAAATAAATAAATAAATAAAAGCGTTTATTAAAACGACCGCGTAACTCTGAGTTGACTCGTTCGAGTTAAGAAACAAATAAACTCAGTCAATCTCCATTCTGCTGCTTCGAACCTTCCTCACCTTTTCAGTCTCTCTATAAATTCCTCTTTGCCGCCATAGCCATTACCAAACTCAAGCTTTTCTTTCTCCTTTTCCAAAGATGAAAAACCATTTCTTCAAGTCTCTTACGCCTTCGCATTCTCCTGCCCGATCGACGCCGTCTCATTCCCCTGCCCGAACGACGCCGTCGCATGGCTTCTGCGACTCTCTCATGGAGGAGAACATCGAAGTTGCAGAAACCCTAATTACGAAGTGGGACTATGCTGCTTCCTCTTACGCCGATATCACTCCTCTCTTCCAAGACGATCGCTACGAGGCCAAGCAGTACATCAAGGCTGTGAAAGATTTGCAGACGGCGATGCAGCATTTCGGCTTCGAGCATTCCAACTCTCAACATCTTGTTCGCGCTCAGAATCTGATGCAGACCGCTATGAAGAGGTTGCAGAGGGAGTTTCACCGGATTCTGACTGAGAATCGGATACATCTGGATCCGGAATCGATTTCGAATCGGTCGTCTAGGGATTCGGTTTTCACTGGTAACTCTGATGTGGAGGATGAGTCTGAGGACGATTTGCGATTTGCGAACGAGAATGTTCCTGAGGAGGAGCGGATGTCCAGATCGGCTATAGCGGATTTGAAATTGATTGCGGAAGGTATGATTTCTGTTGGTTATGGTAAAGAGTGTGGGAAGATTTACACCGTTAGTCGGAAATCGATTGTTGAGGAAGGTTTGTACAATCTCGGAGTTGCGAAGACGAGTTACCATCATGTTCATAGGATGGAGTGGGAGGTTTTGGAGGTGAAGATCAAGAACTGGCTGAACGCTGTGAAAATTGCTGTTAAAACTTTCTTCGAAGCAGAGAGATTCCTCTGCGATCAAGTTTTTTCGATTTCCGCTTCGATTAGAGAGTCTGTTTTCTCAGAAATTACCAAAGAGAGCGCTCTGACTCTGTTTGGCTTCCCGGAAATGGCGGTGAAATCGAAGAAAACTCCCGAGAAAATTTTCCTGATTCTCGATCTGTACGAAGCGATTTTCGACCTTTTGCCGGAGATTGAATCGATGTTCTCCTGCGAATCGACGTCCTCCGTCCGATCTGTAATCGACCATTCGCTAACAAAAATCGCCGAAAGCATCCGCTCCATGCTCGTCGATTTCGAAGCTCACATCCACAAAGATTCCTCCAAAACGCCAGTGCCGAGCGGCGGAATTCATCCGCTCACTCGCTACGTGATGAACTACATCGCATTCCTCAGCGATTACAGCGGAATCCTCCCCGGAATAGTCGCCGACTGGCCGTTGACGCTTCATTCTCCATTGCCGGAATCCTTCTTCGGCGGCAACGATTCGGAAGAAAATCCTCTCACCATACGCTTGGCCTGGATGATTCTGGTCCTCCTCTCGAAGCTCGACAGCAAAGCGGAGCTCTACCACGACGCGCCTCTGTCGTACATCTTCTTGGCAAACAACCTCGAGTACATCGTCGTGAAGGTACGAACCTCGAATCTCAGATTCCTTTTAGGAGACGAGTGGATCGAGAGCCACGAGGCGAAGGTGAAGCAGTACGCATCGAGCTACCAACGAATGGGATGGAGCAGAGTGTTCCTATCGCTGCCGGAGAATCCGACGGCGGAGATCTCACCAGAGAGAGCAAGAAAACACTTCCACGATTTCAACATGGCATTCGAAGAAGCCTACAGGCATCAAGCCTCGTGGATCGTGACGGATCCGAAGCTGCGCGAGCACATCAAGATCTCGTTGGGGAAGAAACTTGGCACGCTCTACGGTGAGTTTTACATCAAATACCGTTCGCGATTGGAGAATCTTTATGGATTGGATTCGGAGGTGAGATTTGCGCCTGATGATTTGGGAAACTATTTGTCTGATTTGCTTCATGGGGATGGAAATTTGGGAGGGAGTGTTTCTTCATCGTCGTCGTCGTCGACGTCGCCGTATCATTTCCACGGTGGTCGGCGGAGTGGATAAGGCCGGCGGTGGAAGAGGAGGTTTTAGGATAAGGACAAATTTAAGAAGTGTCGCTGGATATAAATTTTCAGACGTGTAAAAAATCAAATTGAGTCTGTTTTTTCTTTTTGATTGATTGATTAATTTGTATGGATTGGAATGGAATGGACGTAATGTAAATTCTCTTATTAGATGTTAACTTATTACTTTAGGACACATGTTAGTGGAGTTGGGTATGTGGACCTTTTTTTGTGAAATCATTATCTTCGTGATTTCTTTTCTTTGTTTTTTTTTTAAAAAAGAGTTATC

Coding sequence (CDS)

ATGAAAAACCATTTCTTCAAGTCTCTTACGCCTTCGCATTCTCCTGCCCGATCGACGCCGTCTCATTCCCCTGCCCGAACGACGCCGTCGCATGGCTTCTGCGACTCTCTCATGGAGGAGAACATCGAAGTTGCAGAAACCCTAATTACGAAGTGGGACTATGCTGCTTCCTCTTACGCCGATATCACTCCTCTCTTCCAAGACGATCGCTACGAGGCCAAGCAGTACATCAAGGCTGTGAAAGATTTGCAGACGGCGATGCAGCATTTCGGCTTCGAGCATTCCAACTCTCAACATCTTGTTCGCGCTCAGAATCTGATGCAGACCGCTATGAAGAGGTTGCAGAGGGAGTTTCACCGGATTCTGACTGAGAATCGGATACATCTGGATCCGGAATCGATTTCGAATCGGTCGTCTAGGGATTCGGTTTTCACTGGTAACTCTGATGTGGAGGATGAGTCTGAGGACGATTTGCGATTTGCGAACGAGAATGTTCCTGAGGAGGAGCGGATGTCCAGATCGGCTATAGCGGATTTGAAATTGATTGCGGAAGGTATGATTTCTGTTGGTTATGGTAAAGAGTGTGGGAAGATTTACACCGTTAGTCGGAAATCGATTGTTGAGGAAGGTTTGTACAATCTCGGAGTTGCGAAGACGAGTTACCATCATGTTCATAGGATGGAGTGGGAGGTTTTGGAGGTGAAGATCAAGAACTGGCTGAACGCTGTGAAAATTGCTGTTAAAACTTTCTTCGAAGCAGAGAGATTCCTCTGCGATCAAGTTTTTTCGATTTCCGCTTCGATTAGAGAGTCTGTTTTCTCAGAAATTACCAAAGAGAGCGCTCTGACTCTGTTTGGCTTCCCGGAAATGGCGGTGAAATCGAAGAAAACTCCCGAGAAAATTTTCCTGATTCTCGATCTGTACGAAGCGATTTTCGACCTTTTGCCGGAGATTGAATCGATGTTCTCCTGCGAATCGACGTCCTCCGTCCGATCTGTAATCGACCATTCGCTAACAAAAATCGCCGAAAGCATCCGCTCCATGCTCGTCGATTTCGAAGCTCACATCCACAAAGATTCCTCCAAAACGCCAGTGCCGAGCGGCGGAATTCATCCGCTCACTCGCTACGTGATGAACTACATCGCATTCCTCAGCGATTACAGCGGAATCCTCCCCGGAATAGTCGCCGACTGGCCGTTGACGCTTCATTCTCCATTGCCGGAATCCTTCTTCGGCGGCAACGATTCGGAAGAAAATCCTCTCACCATACGCTTGGCCTGGATGATTCTGGTCCTCCTCTCGAAGCTCGACAGCAAAGCGGAGCTCTACCACGACGCGCCTCTGTCGTACATCTTCTTGGCAAACAACCTCGAGTACATCGTCGTGAAGGTACGAACCTCGAATCTCAGATTCCTTTTAGGAGACGAGTGGATCGAGAGCCACGAGGCGAAGGTGAAGCAGTACGCATCGAGCTACCAACGAATGGGATGGAGCAGAGTGTTCCTATCGCTGCCGGAGAATCCGACGGCGGAGATCTCACCAGAGAGAGCAAGAAAACACTTCCACGATTTCAACATGGCATTCGAAGAAGCCTACAGGCATCAAGCCTCGTGGATCGTGACGGATCCGAAGCTGCGCGAGCACATCAAGATCTCGTTGGGGAAGAAACTTGGCACGCTCTACGGTGAGTTTTACATCAAATACCGTTCGCGATTGGAGAATCTTTATGGATTGGATTCGGAGGTGAGATTTGCGCCTGATGATTTGGGAAACTATTTGTCTGATTTGCTTCATGGGGATGGAAATTTGGGAGGGAGTGTTTCTTCATCGTCGTCGTCGTCGACGTCGCCGTATCATTTCCACGGTGGTCGGCGGAGTGGATAA

Protein sequence

MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYADITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHRILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHGDGNLGGSVSSSSSSSTSPYHFHGGRRSG
Homology
BLAST of Lcy10g002450 vs. ExPASy Swiss-Prot
Match: Q8VY27 (Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=1 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 3.3e-142
Identity = 292/623 (46.87%), Postives = 391/623 (62.76%), Query Frame = 0

Query: 2   KNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYAD 61
           K  FF S +P + P+  +   S   +  +  F  ++MEE +E A+++I KWD    S+  
Sbjct: 6   KFSFFSSSSPKYHPSSPSSFTSFPASPLNQNFTQAMMEETVETADSVIRKWDPNTPSFTK 65

Query: 62  ITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHRI 121
           I  LF   R EAK++I+ V+DL+ AM     + S S  L  AQ LMQ AM RL++EF +I
Sbjct: 66  IVSLFNHSRKEAKEFIRCVRDLRRAMHFLVSQDSQSPKLALAQTLMQIAMARLEKEFFQI 125

Query: 122 LTENRIHLDPESISNRS--SRDSVFTG--NSDVEDESEDDLRFANENVPEEERMSRSAIA 181
           L+ NR  LDPES+S +S  S +S F     SD +DES++++R A E++ + E+ S   ++
Sbjct: 126 LSSNRDKLDPESVSGQSSISSNSEFEDVMGSDDDDESDNEMRKAGESITQVEKASAMVMS 185

Query: 182 DLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIK 241
           DLK IAE MIS GYGKEC KIY   RKSIV+EGL  LG+        HR +W  LE  IK
Sbjct: 186 DLKAIAESMISCGYGKECVKIYKRVRKSIVDEGLSLLGIEIYKGSRFHRTDWVTLEHMIK 245

Query: 242 NWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKT 301
           NW+ A KI + T F  E+ LCD VFS S S RES F EI  E+A  LF FPE   K KK+
Sbjct: 246 NWIKAAKIGIATLFRGEKLLCDHVFSASNSTRESCFYEIANEAATNLFKFPEFVAKEKKS 305

Query: 302 PEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIH 361
            E+IF ++DL  AI DL  +IE +F  ++ + V+S    SL K+  SI S L DFE+ I 
Sbjct: 306 HERIFPLMDLQAAISDLWQDIEMIFHFDAVAGVKSQALTSLQKLKVSIHSALTDFESIIQ 365

Query: 362 KDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSE 421
           KD++K   P GGIH LTR  MN+I+ LS YSG+L  I+AD PL  ++ L ES+     SE
Sbjct: 366 KDTTKALTPGGGIHKLTRSTMNFISSLSKYSGVLSEILADHPLPRNTRLLESYVRAPISE 425

Query: 422 E----NPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLL 481
           +    + L++  AW+ILVLL KLD+KAE Y D  LSY+FLANNL+ I+  V ++ LR LL
Sbjct: 426 DEQHNHALSVHFAWLILVLLCKLDTKAEHYKDVSLSYLFLANNLQIIIETVGSTPLRNLL 485

Query: 482 GDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYR 541
           GD+W+  HE K+  YA +Y+   WS VF+SLPE PT ++SPE A+ +F  F+ AFEEAY 
Sbjct: 486 GDDWLNKHEDKLCAYAGNYEIAAWSNVFMSLPEEPT-DLSPEEAKIYFRRFHTAFEEAYM 545

Query: 542 HQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYL 601
            Q+S +V + KLR+ +K+S+ KKL   Y EFY KY   L     ++  VRF PD+L NY+
Sbjct: 546 KQSSRVVPNAKLRDELKVSIAKKLVPEYREFYRKYLPMLGQERNIEILVRFKPDNLENYI 605

Query: 602 SDLLHGDGNLGGSVSSSSSSSTS 617
           SDL HG      S  SSSSSS+S
Sbjct: 606 SDLFHGTPIHASSSFSSSSSSSS 627

BLAST of Lcy10g002450 vs. ExPASy Swiss-Prot
Match: Q9FGH9 (Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.5e-73
Identity = 162/517 (31.33%), Postives = 276/517 (53.38%), Query Frame = 0

Query: 100 LVRAQNLMQTAMKRLQREFHRILTENRIHLDPESISNRSSRDS-VFTGNSDVEDESEDDL 159
           L RA ++MQ AM R++ EF  ++         ES       D+       D E+E +DD 
Sbjct: 119 LTRADDMMQQAMFRIEEEFRSLMERG-----AESFGLNPQGDAGAMNHRFDSEEEEDDDR 178

Query: 160 RFANEN---VPEEERMS----------RSAIADLKLIAEGMISVGYGKECGKIYTVSRKS 219
            F N +   +P  + ++           + I DL  +A+ M+  G+GK C  +Y+  R+ 
Sbjct: 179 DFNNGDDIQIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSSCRRE 238

Query: 220 IVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSIS 279
            +EE +  LG+ K S   VH+M W+ LE +I  W+ A  +A++  F +ER LCD+VF   
Sbjct: 239 FLEESMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRVFFGF 298

Query: 280 ASIRESVFSEITKESALTLFGFPEMAVKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCE 339
           +S  +  F E+ + S + L  F +      ++PE++F +LD++E + DL+PE ES+FS +
Sbjct: 299 SSAADLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESVFSDQ 358

Query: 340 STSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLS 399
             S +R+       ++ E+IR + ++ E  I +D +K  VP GG+HP+TRYVMNY+    
Sbjct: 359 FCSVLRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYLRAAC 418

Query: 400 DYSGILPGIVADWPLTLHSPLPESFFGGNDSEENPLTIRLAWMILVLLSKLDSKAELYHD 459
                L  +             E   G    +   LT++++W++ +L S L+ K+++Y D
Sbjct: 419 RSRQTLEQVF------------EESNGVPSKDSTLLTVQMSWIMELLESNLEVKSKVYKD 478

Query: 460 APLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIESHEAKVKQYASSYQRMGWSRVF-LSL 519
             L Y+FL NN  YIV KV+  +L  LLGD+WI  H  KVKQY  +YQR  W+++  L  
Sbjct: 479 PALCYVFLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKMLGLLK 538

Query: 520 PENPTAEIS--PERARKHFHDFNMAFEEAYRHQASWIVTDPKLREHIKISLGKKLGTLYG 579
            +N  A ++   +  ++    FN+ F+E  +  ++W+V D +L+E +KISL + L   YG
Sbjct: 539 VDNTAAGMNGLGKTMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLLVPAYG 598

Query: 580 EFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHG 600
            F  ++++  +     D  +++  +D+   +++L  G
Sbjct: 599 SFIGRFQNLGDIGKNADKYIKYGVEDIEARINELFKG 618

BLAST of Lcy10g002450 vs. ExPASy Swiss-Prot
Match: Q9LZD3 (Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 6.0e-72
Identity = 169/585 (28.89%), Postives = 301/585 (51.45%), Query Frame = 0

Query: 38  MEENIEVAETLITKWDYAASSYADITPLFQDDRYEAKQYIKAVKDLQTAMQHF----GFE 97
           ++  ++ AE +++++D    +    T + +    + + Y+ A+  L+  +++F     F+
Sbjct: 66  IDRTLKAAEVILSQFDLLRQAE---TKVLKGPHEDLESYLDAIAQLRKIIRYFMSNKSFK 125

Query: 98  HSNSQHLVRAQNLMQTAMKRLQREFHRILTENRIHLDPESI------SNRSSRDSVFTGN 157
            S+   L  A +L+  A  +L+ EF ++L      ++P+ +      S R S D    G 
Sbjct: 126 SSDGV-LNHANSLLAKAQSKLEEEFKQLLASYSKAVEPDRLFDGLPNSLRPSSDG-DGGG 185

Query: 158 SDVEDESEDDLRFANENVPEEERMSRSAIADLKLIAEGMISVGYGKECGKIYTVSRKSIV 217
                   DD   A   +P    +    +  L  +A+ M+  G+ ++  +IY  +R  ++
Sbjct: 186 KPHGGHHNDDAETAAYTLP--ILIPSRVLPLLHDLAQQMVQAGHQQQLLQIYRDTRSFVL 245

Query: 218 EEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSISAS 277
           EE L  LGV K S   V RM+WEVLE KI NW++ ++IAVK  F  ER +CDQ+F    S
Sbjct: 246 EESLKKLGVEKLSKEDVQRMQWEVLEAKIGNWIHFMRIAVKLLFAGERQVCDQIFRGFDS 305

Query: 278 IRESVFSEITKESALTLFGFPEMAVKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCEST 337
           + +  F+E+T  S   L  F +   +SK++PEK+F++LD+YE + +L  EIE++F  ++ 
Sbjct: 306 LSDQCFAEVTVSSVSMLLSFGDAIARSKRSPEKLFVLLDMYEIMRELHTEIETIFKGKAC 365

Query: 338 SSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDY 397
             +R        ++A++ +    DFE  + KD++KT V  G +HPLT YV+NY+ FL DY
Sbjct: 366 LEIRDSATGLTKRLAQTAQETFGDFEEAVEKDATKTAVLDGTVHPLTSYVINYVKFLFDY 425

Query: 398 SGILPGIVADWPLTLHSPLPESFFGGNDSEENPLTIRLAWMILVLLSKLDSKAELYHDAP 457
              L  +  +             FG  D   + L      ++  L + LD K++ Y D  
Sbjct: 426 QTTLKQLFLE-------------FGNGDDSNSQLASVTMRIMQALQNNLDGKSKQYKDPA 485

Query: 458 LSYIFLANNLEYIVVKVRTSNLRFLLGDEWIESHEAKVKQYASSYQRMGWSRVFL----- 517
           L+++FL NN+ Y+V  VR S  + LLGD+W++ H   V+Q+A+ Y+R+ W+++       
Sbjct: 486 LTHLFLMNNIHYMVRSVRRSEAKDLLGDDWVQRHRRIVQQHANQYKRVAWTKILQSSSAQ 545

Query: 518 --------SLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIVTDPKLREHIKISLG 577
                   SL    ++ +S    ++ F  FNM F+E ++ Q+ W V D +LRE +++++ 
Sbjct: 546 GLTSSGGGSLEGGNSSGVSRGLLKERFKMFNMQFDELHQRQSQWTVPDTELRESLRLAVA 605

Query: 578 KKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHG 600
           + L   Y  F  ++   +E+       +++  +DL   L +L  G
Sbjct: 606 EVLLPAYRSFLKRFGPLVESGKNPQKYIKYTAEDLERLLGELFEG 630

BLAST of Lcy10g002450 vs. ExPASy Swiss-Prot
Match: Q9LMJ4 (Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.8e-61
Identity = 161/550 (29.27%), Postives = 274/550 (49.82%), Query Frame = 0

Query: 26  RTTPSHGFCDSLMEENIEVAETLITKWDYAASSYADITPLFQDDRYEAKQYIKAVKDLQT 85
           R TPS G        N+     L +     +S   +  P++ D   +++ ++ +V +L  
Sbjct: 48  RPTPSRG------GSNVNFDRALTSLERQISSYIVEDRPIWSDP-VDSRTFLDSVDELLA 107

Query: 86  A---MQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHRILTENRIHLDPESISNRSSRDS 145
               ++    + S +    RA  L+Q  M RLQ EF  ++        P+S  +    D 
Sbjct: 108 IAGDLRSMAGDKSVAVCQSRADELIQQVMFRLQEEFGFVMDR-----APDSFDS----DD 167

Query: 146 VFTGNSDVEDESEDDLRFANENVPEE---ERMSRSAIADLKLIAEGMISVGYGKECGKIY 205
            F G  D  +++ D +  A      +   E +  S I DL  IA  M++ G+ KEC ++Y
Sbjct: 168 EFPGEED--NDTSDGVIVARPITDYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVY 227

Query: 206 TVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWLNAVKIAVKTFFEAERFLCD 265
           +  R+  +EE L  L +   S   V    W+ LE +I  W+ AV +    FF +ER LCD
Sbjct: 228 SSRRREFLEESLSRLHLRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCD 287

Query: 266 QVFS--ISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEKIFLILDLYEAIFDLLPE 325
           +VFS    +S+ +  F E+ + +   L  F +      + PE++F ++DLYEA+ DL+P+
Sbjct: 288 RVFSDLPVSSVTDLSFMEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPK 347

Query: 326 IESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDSSKTPVPSGGIHPLTRYV 385
           +E++FS    S +R        ++ E+IR + ++ E  I +D  KT  P GGIHP+TRYV
Sbjct: 348 METLFSDRYCSPLRHEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYV 407

Query: 386 MNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGND--SEENPLTIRLAWMILVLLSK 445
           MNY+         L  I+                 GN+  S+  PL++++ W++ +L S 
Sbjct: 408 MNYLRAACKSRQSLEQILDQ--------------TGNETGSDTRPLSVQIIWVLELLESN 467

Query: 446 LDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIESHEAKVKQYASSYQRM 505
           L+ K   Y D  L ++F+ NN +YI+ K + + L  +LG++WI  H AK++QY S+Y+R 
Sbjct: 468 LEGKKRTYRDPSLCFLFMMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSNYRRS 527

Query: 506 GWSRVF-LSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIVTDPKLREHIKISLG 565
            W++V  L   + P  ++      ++   F   F+E  + Q+ W+V+D +LRE ++ S+ 
Sbjct: 528 SWNQVVGLLRTDGPYPKLV-----ENLRLFKSQFDEVCKVQSQWVVSDGQLREELRSSVA 560

BLAST of Lcy10g002450 vs. ExPASy Swiss-Prot
Match: F4KG58 (Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.8e-60
Identity = 129/425 (30.35%), Postives = 225/425 (52.94%), Query Frame = 0

Query: 182 IAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWLN 241
           +A+ M+  G+ +E  K Y   R++++ + L  LGV + S + V RM  +V E KI NW++
Sbjct: 167 LAQQMVKAGHQQELFKTYRDIRRAVLAQSLEKLGVERHSKYDVERMNQDVFEAKIMNWIH 226

Query: 242 AVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEKI 301
            ++I+VK  F AE+ +C Q+       R+  F+EIT  S   L  F      S+++PEK+
Sbjct: 227 YIRISVKLLFAAEKEICHQILDGVEPFRDQSFAEITTISFGMLLSFGYAIAISRRSPEKV 286

Query: 302 FLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDSS 361
           F+ILD+YE + +L PE E +F  +  + ++    +    +A++++  + DFE  I  D++
Sbjct: 287 FVILDMYEIMIELQPEFELIFGSKPCTEMKEDALNLTKLLAQTVKETIADFEVAIEMDAT 346

Query: 362 KTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENPL 421
           +T V  G +H LT YV  Y+ FL DY   L  +  +             F  ND +    
Sbjct: 347 ETVVMDGSVHALTSYVARYVKFLFDYEPTLRQLFQE-------------FNSNDPDTKLK 406

Query: 422 TIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIESH 481
           ++ +  ++  L + LD K+  + DA L+ +FL NN+ YIV   R    +  LGD+ +++H
Sbjct: 407 SV-MTGIMRALRNNLDGKSRQFEDAALTQLFLMNNVYYIVRNFRREEAKNFLGDDLVQTH 466

Query: 482 EAKVKQYASSYQRMGWSRVF--LSLPENPTAEISPERARK-----HFHDFNMAFEEAYRH 541
              V+Q+A  YQ + W+++   +++  + +  I  E  +K      F  FN  FEE ++ 
Sbjct: 467 RRIVQQHAKQYQTISWNKILQCITVQSSKSGLIKNESIKKTLVKEKFKTFNSQFEELHQR 526

Query: 542 QASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLS 600
           Q  W V+D +LRE +++++ + L   YG F  ++   +E+       +RF P+DL   L+
Sbjct: 527 QCQWSVSDVELRESLRLAIAEVLLPAYGSFLKRFGPMIESGKNSQKYIRFTPEDLERMLN 577

BLAST of Lcy10g002450 vs. ExPASy TrEMBL
Match: A0A6J1G974 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111452086 PE=3 SV=1)

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 568/636 (89.31%), Postives = 596/636 (93.71%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPAR---------TTPSHGFCDSLMEENIEVAETLITK 60
           M+NH FKSLTPSHSPAR+TPSHSPAR         TTPSH   +SLMEENI+VAE LITK
Sbjct: 1   MRNHLFKSLTPSHSPARTTPSHSPARSTPSRSSAPTTPSHALSESLMEENIDVAEILITK 60

Query: 61  WDYAASSYADITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAM 120
           WD+A SSYADITPLFQ+DRYEAKQY+KAVKDLQTAM++FG EH  S+HLVRAQNLMQTAM
Sbjct: 61  WDHATSSYADITPLFQEDRYEAKQYLKAVKDLQTAMKYFGSEHMKSRHLVRAQNLMQTAM 120

Query: 121 KRLQREFHRILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERM 180
           KRLQREFHRIL+ENR HLDPESISNRSSRDS  TGNSD+EDESEDDLRFANE+V EEER+
Sbjct: 121 KRLQREFHRILSENRAHLDPESISNRSSRDSGLTGNSDLEDESEDDLRFANEDVTEEERI 180

Query: 181 SRSAIADLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240
           SRS IADLK IAEGMIS GY KEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEV
Sbjct: 181 SRSVIADLKSIAEGMISAGYSKECVKIYTVTRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240

Query: 241 LEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMA 300
           LEVKIKNWLNAVKIAVK FFE E+FLCDQVFSIS  IRESVFSEIT+ESALTLFGFPEMA
Sbjct: 241 LEVKIKNWLNAVKIAVKNFFEPEKFLCDQVFSISDMIRESVFSEITRESALTLFGFPEMA 300

Query: 301 VKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVD 360
           VKSKKTPEKIFLIL+LYE IFDLLPEIESMFSCEST S+RS+IDHSLTKIAESIRSMLVD
Sbjct: 301 VKSKKTPEKIFLILNLYEVIFDLLPEIESMFSCESTFSIRSLIDHSLTKIAESIRSMLVD 360

Query: 361 FEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFF 420
           FE+HI KDSSKTPVPSGG+HPLTRYVMNYI+FLSDYSGILPGIVADWPL LHSPLPESFF
Sbjct: 361 FESHIQKDSSKTPVPSGGVHPLTRYVMNYISFLSDYSGILPGIVADWPLMLHSPLPESFF 420

Query: 421 GGNDSEENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRF 480
           GGNDSEENPLTIRLAW+ILVLLSKLDSKAELYHDAPLSYIFLANNL+YIVVK RTSNL F
Sbjct: 421 GGNDSEENPLTIRLAWIILVLLSKLDSKAELYHDAPLSYIFLANNLKYIVVKARTSNLTF 480

Query: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEA 540
           LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTA+ISPERARKHFHDFN+AFEEA
Sbjct: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTADISPERARKHFHDFNIAFEEA 540

Query: 541 YRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGN 600
           YRHQASWIVTD KLREHIKISLGKKLGTLYGEFYIKYRSRLENLYG DSEVRFAPDDLGN
Sbjct: 541 YRHQASWIVTDSKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGCDSEVRFAPDDLGN 600

Query: 601 YLSDLLHGDGNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           YLSDLLHGDGNLGGSVSSSSS S+SPYHFHGGRRSG
Sbjct: 601 YLSDLLHGDGNLGGSVSSSSSLSSSPYHFHGGRRSG 636

BLAST of Lcy10g002450 vs. ExPASy TrEMBL
Match: A0A6J1HIW8 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111464861 PE=3 SV=1)

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 561/627 (89.47%), Postives = 597/627 (95.22%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M+NHFFKSLTPSHSPAR+TPSHSPARTTPSHG C+SLMEENIEVAETLITKWD A+SSYA
Sbjct: 1   MRNHFFKSLTPSHSPARTTPSHSPARTTPSHGLCESLMEENIEVAETLITKWDSASSSYA 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           DITPLFQDDRYEAKQY+KAVKDLQTAM +FG EH+NSQHLVR+Q LMQTAMKRLQREFHR
Sbjct: 61  DITPLFQDDRYEAKQYLKAVKDLQTAMDYFGTEHTNSQHLVRSQRLMQTAMKRLQREFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLK 180
           IL ENR HLDPESISNRSSR+SV TGNSD+ED+SEDDLRFANENV E+ERMSRSAIADLK
Sbjct: 121 ILAENRTHLDPESISNRSSRNSVLTGNSDLEDDSEDDLRFANENVSEDERMSRSAIADLK 180

Query: 181 LIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240
            IAEGMIS GYGKEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL
Sbjct: 181 SIAEGMISAGYGKECVKIYTVNRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240

Query: 241 NAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEK 300
           NAVKIAVKTFFEAE++LCD+VF  SASIRESVFSEITKESALTLFGFPEMAVKSKK+PEK
Sbjct: 241 NAVKIAVKTFFEAEKYLCDRVFWSSASIRESVFSEITKESALTLFGFPEMAVKSKKSPEK 300

Query: 301 IFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDS 360
           IFLILDLYEAIF+L P+IESMF+CEST+SVRS+IDHSLTKIAESIRSMLVDFE+HI KDS
Sbjct: 301 IFLILDLYEAIFNLWPDIESMFACESTASVRSLIDHSLTKIAESIRSMLVDFESHIQKDS 360

Query: 361 SKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENP 420
           S+TPVP GG+HPLTRYVMNYIAFLSDYSGILPGIVADWPL LHSPLPESFFGGNDSEENP
Sbjct: 361 SRTPVPGGGVHPLTRYVMNYIAFLSDYSGILPGIVADWPLMLHSPLPESFFGGNDSEENP 420

Query: 421 LTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIES 480
           L +RLAW+ILVLLSKLDSKAE+YHDAPLSYIFLANNLEYIVVKVRTSNL+ +LGDEWIES
Sbjct: 421 LALRLAWLILVLLSKLDSKAEVYHDAPLSYIFLANNLEYIVVKVRTSNLKSVLGDEWIES 480

Query: 481 HEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIV 540
           HEAKVKQYASSY RMGWSRVFLSLPENP AEISPERARKHFHDFN+AFEEAY+HQASWIV
Sbjct: 481 HEAKVKQYASSYIRMGWSRVFLSLPENPMAEISPERARKHFHDFNIAFEEAYKHQASWIV 540

Query: 541 TDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHGD 600
            D KLREHIKI+LGKKLGTLYGEFYI YR+RLENLYG DSEVRFAPDDLGNYLSDLLH D
Sbjct: 541 MDSKLREHIKITLGKKLGTLYGEFYINYRARLENLYGNDSEVRFAPDDLGNYLSDLLHAD 600

Query: 601 GNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           GN GGSVSS+SS S+SPYHFHGGR SG
Sbjct: 601 GNSGGSVSSTSSLSSSPYHFHGGRHSG 627

BLAST of Lcy10g002450 vs. ExPASy TrEMBL
Match: A0A6J1I2W5 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111469425 PE=3 SV=1)

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 561/627 (89.47%), Postives = 595/627 (94.90%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M+NHFFKSLTPSHSPAR+TPSHSPARTTPSHG C+SLMEENIEVAETLITKWD A+SSYA
Sbjct: 1   MRNHFFKSLTPSHSPARTTPSHSPARTTPSHGLCESLMEENIEVAETLITKWDSASSSYA 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           DITPLFQDDRYEAKQY+KAVKDLQTAM +FG EH+NS HLVRAQ LMQTAMKRLQREFHR
Sbjct: 61  DITPLFQDDRYEAKQYLKAVKDLQTAMDYFGTEHTNSHHLVRAQRLMQTAMKRLQREFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLK 180
           IL ENR HLDPESISNRSSR+SV TGNSD+ED+SEDDLRFANENV EEERMSRSAIADLK
Sbjct: 121 ILAENRTHLDPESISNRSSRNSVLTGNSDLEDDSEDDLRFANENVSEEERMSRSAIADLK 180

Query: 181 LIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240
            IAEGMIS GYGKEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL
Sbjct: 181 SIAEGMISAGYGKECVKIYTVNRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240

Query: 241 NAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEK 300
           NAVKIAVKTFFEAE++LCD+VF  SASIRESVFSEITK+SALTLFGFPEMAVKSKK+PEK
Sbjct: 241 NAVKIAVKTFFEAEKYLCDRVFWSSASIRESVFSEITKDSALTLFGFPEMAVKSKKSPEK 300

Query: 301 IFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDS 360
           IFLILDLYEAIF+L P+IESMF+CEST+SVRS+IDHSLTKIAESIRSMLVDFE+HI KDS
Sbjct: 301 IFLILDLYEAIFNLWPDIESMFACESTASVRSLIDHSLTKIAESIRSMLVDFESHIQKDS 360

Query: 361 SKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENP 420
           S+TPVP GG+HPLTRYVMNYIAFLSDYSG+LPGIVADWPL LHS LPESFFGGNDSEENP
Sbjct: 361 SRTPVPGGGVHPLTRYVMNYIAFLSDYSGVLPGIVADWPLMLHSTLPESFFGGNDSEENP 420

Query: 421 LTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIES 480
           L +RLAW+ILVLLSKLDSKAE+YHDAPLSYIFLANNLEYIVVKVRTSNLR +LGDEWIES
Sbjct: 421 LALRLAWLILVLLSKLDSKAEVYHDAPLSYIFLANNLEYIVVKVRTSNLRSVLGDEWIES 480

Query: 481 HEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIV 540
           HEAKVKQYASSY RMGWSRVFLSLPENP  EISPERARKHFHDFN+AFEEAY+HQASWIV
Sbjct: 481 HEAKVKQYASSYIRMGWSRVFLSLPENPMGEISPERARKHFHDFNIAFEEAYKHQASWIV 540

Query: 541 TDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHGD 600
           TD KLREHIKI+LGKKLGTLYGEFYI YR+RLENLYG DSEVRFAPDDLGNYLSDLLH D
Sbjct: 541 TDSKLREHIKITLGKKLGTLYGEFYINYRARLENLYGNDSEVRFAPDDLGNYLSDLLHAD 600

Query: 601 GNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           GN GGSVSS SS S+SPYHFHGGRRSG
Sbjct: 601 GNSGGSVSSMSSLSSSPYHFHGGRRSG 627

BLAST of Lcy10g002450 vs. ExPASy TrEMBL
Match: A0A6J1KHD6 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111493246 PE=3 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 563/636 (88.52%), Postives = 594/636 (93.40%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPAR---------TTPSHGFCDSLMEENIEVAETLITK 60
           M+NHFF+SLTPSHSPAR+TPSHSP R         TTPSH   +SLMEENI+VAE LITK
Sbjct: 1   MRNHFFRSLTPSHSPARTTPSHSPTRSTPSRSSAPTTPSHALSESLMEENIDVAEILITK 60

Query: 61  WDYAASSYADITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAM 120
           WD A SSYADITPLFQ+D YEAKQY+KAVKDLQTAM++FG E+  S+HLVRAQNLMQTAM
Sbjct: 61  WDRAISSYADITPLFQEDGYEAKQYLKAVKDLQTAMKYFGSENMKSRHLVRAQNLMQTAM 120

Query: 121 KRLQREFHRILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERM 180
           KRLQREFHRIL++NR HLDPESISNRSSRDS  TGNSD+EDESEDDLRFANE+V EEER+
Sbjct: 121 KRLQREFHRILSDNRAHLDPESISNRSSRDSGLTGNSDLEDESEDDLRFANEDVTEEERI 180

Query: 181 SRSAIADLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240
           SRS IADLK IAEGMIS GY KEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEV
Sbjct: 181 SRSVIADLKSIAEGMISAGYSKECVKIYTVTRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240

Query: 241 LEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMA 300
           LEVKIKNWLNAVKIAVK FFE E+FLCDQVFSIS  IRESVFSEIT+ESALTLFGFPEMA
Sbjct: 241 LEVKIKNWLNAVKIAVKNFFEPEKFLCDQVFSISDMIRESVFSEITRESALTLFGFPEMA 300

Query: 301 VKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVD 360
           VKSKKTPEKIFLIL+LYE IFDLLPEIESMFSCESTSS+RS+IDHSLTKIAESIRSMLVD
Sbjct: 301 VKSKKTPEKIFLILNLYEVIFDLLPEIESMFSCESTSSIRSLIDHSLTKIAESIRSMLVD 360

Query: 361 FEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFF 420
           FE+HI KDSSKTPVPSGG+HPLTRYVMNYI+FLSDYSGILPGIVADWPL LHSPLPESFF
Sbjct: 361 FESHIQKDSSKTPVPSGGVHPLTRYVMNYISFLSDYSGILPGIVADWPLMLHSPLPESFF 420

Query: 421 GGNDSEENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRF 480
           GGNDSEENPLTIRLAW+ILVLLSKLDSKAELY DAPLSYIFLANNL+YIVVK RTSNLRF
Sbjct: 421 GGNDSEENPLTIRLAWIILVLLSKLDSKAELYLDAPLSYIFLANNLKYIVVKARTSNLRF 480

Query: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEA 540
           LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTA+ISPERARKHFHDFN+AFEEA
Sbjct: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTADISPERARKHFHDFNIAFEEA 540

Query: 541 YRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGN 600
           YRHQASWIVTD KLREHIKISLGKKLGTLYGEFYIKYRSRLE L+G DSEVRFAPDDLGN
Sbjct: 541 YRHQASWIVTDSKLREHIKISLGKKLGTLYGEFYIKYRSRLEKLFGCDSEVRFAPDDLGN 600

Query: 601 YLSDLLHGDGNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           YLSDLLHGDGNLGGSVSSSSS S+SPYHFHGGRRSG
Sbjct: 601 YLSDLLHGDGNLGGSVSSSSSLSSSPYHFHGGRRSG 636

BLAST of Lcy10g002450 vs. ExPASy TrEMBL
Match: A0A0A0LXW3 (Exocyst subunit Exo70 family protein OS=Cucumis sativus OX=3659 GN=Csa_1G481200 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 559/628 (89.01%), Postives = 581/628 (92.52%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M+NHFFK      SPARSTPSHSP    PSHG C SLMEENI+VAETLITKWD  ASSYA
Sbjct: 1   MRNHFFK------SPARSTPSHSP---PPSHGLCQSLMEENIDVAETLITKWDCTASSYA 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           DITPLFQDDRYEA QY+KAVKDLQTAMQ+FG E  NS HLVRAQNLMQTAMKRLQREFHR
Sbjct: 61  DITPLFQDDRYEANQYLKAVKDLQTAMQYFGSERMNSHHLVRAQNLMQTAMKRLQREFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANE-NVPEEERMSRSAIADL 180
           IL ENR HLDPESISNRSSRDSVFTGNSD+EDESEDDLRFANE NV EEER+SRSAI DL
Sbjct: 121 ILAENRAHLDPESISNRSSRDSVFTGNSDLEDESEDDLRFANENNVSEEERISRSAIKDL 180

Query: 181 KLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNW 240
           K IAEGMIS GYGKEC KIY V RKSIVEEGLYNLGVAK +YHHVHRMEWEVLEVKIKNW
Sbjct: 181 KSIAEGMISAGYGKECVKIYIVGRKSIVEEGLYNLGVAKPNYHHVHRMEWEVLEVKIKNW 240

Query: 241 LNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPE 300
           LNAVKIAVKTFFEAE+FL DQVFS SASIRESVFSEITKESALTLF FPEMAVKSKKTPE
Sbjct: 241 LNAVKIAVKTFFEAEKFLSDQVFSSSASIRESVFSEITKESALTLFSFPEMAVKSKKTPE 300

Query: 301 KIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKD 360
           KIFLILDLYEAIFDL PEIESMF  EST+S+RS+IDHSLTKIAESIRSML+DFE+HI KD
Sbjct: 301 KIFLILDLYEAIFDLWPEIESMFIYESTASIRSLIDHSLTKIAESIRSMLIDFESHIQKD 360

Query: 361 SSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEEN 420
           SSKTPVP GG+HPLTRYVMNYIAFLSDYSGILPGIVADWPL LHSPLPESFFGGNDSEEN
Sbjct: 361 SSKTPVPGGGVHPLTRYVMNYIAFLSDYSGILPGIVADWPLLLHSPLPESFFGGNDSEEN 420

Query: 421 PLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIE 480
           PLTIR+AW+ILVLLSKLDSKAE+YHDAPLSYIFLANNLEYIVVKVRTSNLRF+LGDEWIE
Sbjct: 421 PLTIRMAWLILVLLSKLDSKAEIYHDAPLSYIFLANNLEYIVVKVRTSNLRFVLGDEWIE 480

Query: 481 SHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWI 540
           SHE KV+QYASSYQRMGWSRVFLSLPENP AEISPERARKHFHDFN+AFEEAYRHQASWI
Sbjct: 481 SHETKVRQYASSYQRMGWSRVFLSLPENPMAEISPERARKHFHDFNIAFEEAYRHQASWI 540

Query: 541 VTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHG 600
           VTD KLREHIKISLGKKLGTLYGEFYI  RSRLENLYG +SEVRFAPDDLGNYLSDLLHG
Sbjct: 541 VTDSKLREHIKISLGKKLGTLYGEFYISNRSRLENLYGSESEVRFAPDDLGNYLSDLLHG 600

Query: 601 DGNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           DGN GGSVSSSSS S+SPYHFHGGRRSG
Sbjct: 601 DGNSGGSVSSSSSLSSSPYHFHGGRRSG 619

BLAST of Lcy10g002450 vs. NCBI nr
Match: KAG6607205.1 (Exocyst complex component EXO70H1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 568/636 (89.31%), Postives = 597/636 (93.87%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPAR---------TTPSHGFCDSLMEENIEVAETLITK 60
           M+NHFFKSLTPSHSPAR+TPSHSPAR         TTPSH   +SLMEENI+VAE LITK
Sbjct: 1   MRNHFFKSLTPSHSPARTTPSHSPARSTPSRSSAPTTPSHALSESLMEENIDVAEILITK 60

Query: 61  WDYAASSYADITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAM 120
           WD+A SSYADITPLFQ+DRYEAKQY+KAVKDLQTAM++FG E   S+HLVRAQNLMQTAM
Sbjct: 61  WDHATSSYADITPLFQEDRYEAKQYLKAVKDLQTAMKYFGSEQMKSRHLVRAQNLMQTAM 120

Query: 121 KRLQREFHRILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERM 180
           KRLQREFHRIL+ENR HLDPESISNRSSRDS  TGNSD+EDESEDDLRFANE+V EEER+
Sbjct: 121 KRLQREFHRILSENRAHLDPESISNRSSRDSGLTGNSDLEDESEDDLRFANEDVTEEERI 180

Query: 181 SRSAIADLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240
           SRS IADLK IAEGMIS GY KEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEV
Sbjct: 181 SRSVIADLKSIAEGMISAGYSKECVKIYTVTRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240

Query: 241 LEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMA 300
           LEVKIKNWLNAVKIAVK FFE E+FLCD+VFSIS  IRESVFSEITKESALTLFGFPEMA
Sbjct: 241 LEVKIKNWLNAVKIAVKNFFEPEKFLCDEVFSISDMIRESVFSEITKESALTLFGFPEMA 300

Query: 301 VKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVD 360
           VKSKKTPEKIFLIL+LYE IFDLLPEIESMFSCESTSS+RS+IDHSLTKIAESIRSMLVD
Sbjct: 301 VKSKKTPEKIFLILNLYEVIFDLLPEIESMFSCESTSSIRSLIDHSLTKIAESIRSMLVD 360

Query: 361 FEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFF 420
           FE+HI KDSSKTPVPSGG+HPLTRYVMNYI+FLSDYSGILPGIVADWPL LHSPLPESFF
Sbjct: 361 FESHIQKDSSKTPVPSGGVHPLTRYVMNYISFLSDYSGILPGIVADWPLMLHSPLPESFF 420

Query: 421 GGNDSEENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRF 480
           GGNDSEENPLTIRLAW+ILVLLSKLDSKAELYHDAPLSYIFLANNL+YIVVK RTSNL F
Sbjct: 421 GGNDSEENPLTIRLAWIILVLLSKLDSKAELYHDAPLSYIFLANNLKYIVVKARTSNLTF 480

Query: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEA 540
           LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTA+ISPERARKHFHDFN+AFEEA
Sbjct: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTADISPERARKHFHDFNIAFEEA 540

Query: 541 YRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGN 600
           YRHQASWIVTD KLREHIKISLGKKLGTLYGEFY+KYRSRLENLYG DSEVRFAPDDLGN
Sbjct: 541 YRHQASWIVTDSKLREHIKISLGKKLGTLYGEFYLKYRSRLENLYGCDSEVRFAPDDLGN 600

Query: 601 YLSDLLHGDGNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           YLSDLLHGDGNLGGSVSSSSS S+SPYHFHGGRRSG
Sbjct: 601 YLSDLLHGDGNLGGSVSSSSSLSSSPYHFHGGRRSG 636

BLAST of Lcy10g002450 vs. NCBI nr
Match: KAG6583465.1 (Exocyst complex component EXO70H1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 562/627 (89.63%), Postives = 597/627 (95.22%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M+NHFFKSLTPSHSPAR+TPSHSPARTTPSHG C+SLMEENIEVAETLITKWD A+SSYA
Sbjct: 1   MRNHFFKSLTPSHSPARTTPSHSPARTTPSHGLCESLMEENIEVAETLITKWDSASSSYA 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           DITPLFQDDRYEAKQY+KAVKDLQTAM +FG EH+NSQHLVR+Q LMQTAMKRLQREFHR
Sbjct: 61  DITPLFQDDRYEAKQYLKAVKDLQTAMDYFGTEHTNSQHLVRSQRLMQTAMKRLQREFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLK 180
           IL ENR HLDPESISNRSSR+SV TGNSD+ED+SEDDLRFANENV EEERMSRSAIADLK
Sbjct: 121 ILAENRTHLDPESISNRSSRNSVLTGNSDLEDDSEDDLRFANENVSEEERMSRSAIADLK 180

Query: 181 LIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240
            IAEGMIS GYGKEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL
Sbjct: 181 SIAEGMISAGYGKECVKIYTVNRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240

Query: 241 NAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEK 300
           NAVKIAVKTFFEAE++LCD+VF  SASIRESVFSEITKESALTLFGFPEMAVKSKK+PEK
Sbjct: 241 NAVKIAVKTFFEAEKYLCDRVFWSSASIRESVFSEITKESALTLFGFPEMAVKSKKSPEK 300

Query: 301 IFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDS 360
           IFLILDLYEAIF+L P+IESMF+CEST+SVRS+IDHSLTKIAESIRSMLVDFE+HI KDS
Sbjct: 301 IFLILDLYEAIFNLWPDIESMFACESTASVRSLIDHSLTKIAESIRSMLVDFESHIQKDS 360

Query: 361 SKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENP 420
           S+TPVP GG+HPLTRYVMNYIAFLSDYSGILPGIVADWPL LHSPLPESFFGGNDSEENP
Sbjct: 361 SRTPVPGGGVHPLTRYVMNYIAFLSDYSGILPGIVADWPLMLHSPLPESFFGGNDSEENP 420

Query: 421 LTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIES 480
           L +RLAW+ILVLLSKLDSKAE+YHDAPLSYIFLANNLEYIVVKVRTSNL+ +LGDEWIES
Sbjct: 421 LALRLAWLILVLLSKLDSKAEVYHDAPLSYIFLANNLEYIVVKVRTSNLKSVLGDEWIES 480

Query: 481 HEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIV 540
           HEAKVKQYASSY RMGWSRVFLSLPENP AEISPERARKHFHDFN+AFEEAY+HQASWIV
Sbjct: 481 HEAKVKQYASSYIRMGWSRVFLSLPENPMAEISPERARKHFHDFNIAFEEAYKHQASWIV 540

Query: 541 TDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHGD 600
            D KLREHIKI+LGKKLGTLYGEFYI YR+RLENLYG DSEVRFAPDDLGNYLSDLLH D
Sbjct: 541 MDSKLREHIKITLGKKLGTLYGEFYINYRARLENLYGNDSEVRFAPDDLGNYLSDLLHAD 600

Query: 601 GNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           GN GGSVSS+SS S+SPYHFHGGR SG
Sbjct: 601 GNSGGSVSSTSSLSSSPYHFHGGRHSG 627

BLAST of Lcy10g002450 vs. NCBI nr
Match: XP_022948393.1 (exocyst complex component EXO70H1-like [Cucurbita moschata])

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 568/636 (89.31%), Postives = 596/636 (93.71%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPAR---------TTPSHGFCDSLMEENIEVAETLITK 60
           M+NH FKSLTPSHSPAR+TPSHSPAR         TTPSH   +SLMEENI+VAE LITK
Sbjct: 1   MRNHLFKSLTPSHSPARTTPSHSPARSTPSRSSAPTTPSHALSESLMEENIDVAEILITK 60

Query: 61  WDYAASSYADITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAM 120
           WD+A SSYADITPLFQ+DRYEAKQY+KAVKDLQTAM++FG EH  S+HLVRAQNLMQTAM
Sbjct: 61  WDHATSSYADITPLFQEDRYEAKQYLKAVKDLQTAMKYFGSEHMKSRHLVRAQNLMQTAM 120

Query: 121 KRLQREFHRILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERM 180
           KRLQREFHRIL+ENR HLDPESISNRSSRDS  TGNSD+EDESEDDLRFANE+V EEER+
Sbjct: 121 KRLQREFHRILSENRAHLDPESISNRSSRDSGLTGNSDLEDESEDDLRFANEDVTEEERI 180

Query: 181 SRSAIADLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240
           SRS IADLK IAEGMIS GY KEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEV
Sbjct: 181 SRSVIADLKSIAEGMISAGYSKECVKIYTVTRKSIVEEGLYNLGVAKTSYHHVHRMEWEV 240

Query: 241 LEVKIKNWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMA 300
           LEVKIKNWLNAVKIAVK FFE E+FLCDQVFSIS  IRESVFSEIT+ESALTLFGFPEMA
Sbjct: 241 LEVKIKNWLNAVKIAVKNFFEPEKFLCDQVFSISDMIRESVFSEITRESALTLFGFPEMA 300

Query: 301 VKSKKTPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVD 360
           VKSKKTPEKIFLIL+LYE IFDLLPEIESMFSCEST S+RS+IDHSLTKIAESIRSMLVD
Sbjct: 301 VKSKKTPEKIFLILNLYEVIFDLLPEIESMFSCESTFSIRSLIDHSLTKIAESIRSMLVD 360

Query: 361 FEAHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFF 420
           FE+HI KDSSKTPVPSGG+HPLTRYVMNYI+FLSDYSGILPGIVADWPL LHSPLPESFF
Sbjct: 361 FESHIQKDSSKTPVPSGGVHPLTRYVMNYISFLSDYSGILPGIVADWPLMLHSPLPESFF 420

Query: 421 GGNDSEENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRF 480
           GGNDSEENPLTIRLAW+ILVLLSKLDSKAELYHDAPLSYIFLANNL+YIVVK RTSNL F
Sbjct: 421 GGNDSEENPLTIRLAWIILVLLSKLDSKAELYHDAPLSYIFLANNLKYIVVKARTSNLTF 480

Query: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEA 540
           LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTA+ISPERARKHFHDFN+AFEEA
Sbjct: 481 LLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTADISPERARKHFHDFNIAFEEA 540

Query: 541 YRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGN 600
           YRHQASWIVTD KLREHIKISLGKKLGTLYGEFYIKYRSRLENLYG DSEVRFAPDDLGN
Sbjct: 541 YRHQASWIVTDSKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGCDSEVRFAPDDLGN 600

Query: 601 YLSDLLHGDGNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           YLSDLLHGDGNLGGSVSSSSS S+SPYHFHGGRRSG
Sbjct: 601 YLSDLLHGDGNLGGSVSSSSSLSSSPYHFHGGRRSG 636

BLAST of Lcy10g002450 vs. NCBI nr
Match: XP_022964887.1 (exocyst complex component EXO70H1-like [Cucurbita moschata])

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 561/627 (89.47%), Postives = 597/627 (95.22%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M+NHFFKSLTPSHSPAR+TPSHSPARTTPSHG C+SLMEENIEVAETLITKWD A+SSYA
Sbjct: 1   MRNHFFKSLTPSHSPARTTPSHSPARTTPSHGLCESLMEENIEVAETLITKWDSASSSYA 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           DITPLFQDDRYEAKQY+KAVKDLQTAM +FG EH+NSQHLVR+Q LMQTAMKRLQREFHR
Sbjct: 61  DITPLFQDDRYEAKQYLKAVKDLQTAMDYFGTEHTNSQHLVRSQRLMQTAMKRLQREFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLK 180
           IL ENR HLDPESISNRSSR+SV TGNSD+ED+SEDDLRFANENV E+ERMSRSAIADLK
Sbjct: 121 ILAENRTHLDPESISNRSSRNSVLTGNSDLEDDSEDDLRFANENVSEDERMSRSAIADLK 180

Query: 181 LIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240
            IAEGMIS GYGKEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL
Sbjct: 181 SIAEGMISAGYGKECVKIYTVNRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240

Query: 241 NAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEK 300
           NAVKIAVKTFFEAE++LCD+VF  SASIRESVFSEITKESALTLFGFPEMAVKSKK+PEK
Sbjct: 241 NAVKIAVKTFFEAEKYLCDRVFWSSASIRESVFSEITKESALTLFGFPEMAVKSKKSPEK 300

Query: 301 IFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDS 360
           IFLILDLYEAIF+L P+IESMF+CEST+SVRS+IDHSLTKIAESIRSMLVDFE+HI KDS
Sbjct: 301 IFLILDLYEAIFNLWPDIESMFACESTASVRSLIDHSLTKIAESIRSMLVDFESHIQKDS 360

Query: 361 SKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENP 420
           S+TPVP GG+HPLTRYVMNYIAFLSDYSGILPGIVADWPL LHSPLPESFFGGNDSEENP
Sbjct: 361 SRTPVPGGGVHPLTRYVMNYIAFLSDYSGILPGIVADWPLMLHSPLPESFFGGNDSEENP 420

Query: 421 LTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIES 480
           L +RLAW+ILVLLSKLDSKAE+YHDAPLSYIFLANNLEYIVVKVRTSNL+ +LGDEWIES
Sbjct: 421 LALRLAWLILVLLSKLDSKAEVYHDAPLSYIFLANNLEYIVVKVRTSNLKSVLGDEWIES 480

Query: 481 HEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIV 540
           HEAKVKQYASSY RMGWSRVFLSLPENP AEISPERARKHFHDFN+AFEEAY+HQASWIV
Sbjct: 481 HEAKVKQYASSYIRMGWSRVFLSLPENPMAEISPERARKHFHDFNIAFEEAYKHQASWIV 540

Query: 541 TDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHGD 600
            D KLREHIKI+LGKKLGTLYGEFYI YR+RLENLYG DSEVRFAPDDLGNYLSDLLH D
Sbjct: 541 MDSKLREHIKITLGKKLGTLYGEFYINYRARLENLYGNDSEVRFAPDDLGNYLSDLLHAD 600

Query: 601 GNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           GN GGSVSS+SS S+SPYHFHGGR SG
Sbjct: 601 GNSGGSVSSTSSLSSSPYHFHGGRHSG 627

BLAST of Lcy10g002450 vs. NCBI nr
Match: XP_023519812.1 (exocyst complex component EXO70H1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 561/627 (89.47%), Postives = 596/627 (95.06%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M+NHFFKSLTPSHSPAR+TPSHSPARTTPSHG C+SLMEENIEVAETLITKWD A+SSYA
Sbjct: 1   MRNHFFKSLTPSHSPARTTPSHSPARTTPSHGLCESLMEENIEVAETLITKWDSASSSYA 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           DITPLFQDDRYEAKQY+KAVKDLQTAM +FG EH+NSQHLVRAQ LMQTAMKRLQREFHR
Sbjct: 61  DITPLFQDDRYEAKQYLKAVKDLQTAMDYFGTEHTNSQHLVRAQRLMQTAMKRLQREFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLK 180
           IL ENR HLDPESISNRSSR+SV TGNSD+ED+SEDDLRFANENV EEERMSRSAIADLK
Sbjct: 121 ILAENRTHLDPESISNRSSRNSVLTGNSDLEDDSEDDLRFANENVSEEERMSRSAIADLK 180

Query: 181 LIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240
            IAEGMIS GYGKEC KIYTV+RKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL
Sbjct: 181 SIAEGMISAGYGKECVKIYTVNRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWL 240

Query: 241 NAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKTPEK 300
           NAVKIAVKTFFEAE++LCD+VF  SASIRES+FSEITKESALTLFGFPEMAVKSKK+PEK
Sbjct: 241 NAVKIAVKTFFEAEKYLCDRVFWSSASIRESIFSEITKESALTLFGFPEMAVKSKKSPEK 300

Query: 301 IFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDS 360
           IFLILDLYEAIF+L P+IESMF+CEST+SVRS+IDHSLTKIAESIRSMLVDFE+HI KDS
Sbjct: 301 IFLILDLYEAIFNLWPDIESMFACESTASVRSLIDHSLTKIAESIRSMLVDFESHIQKDS 360

Query: 361 SKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENP 420
           S+TPVP GG+HPLTRYVMNYIAFLSDYSGILPGIVADWP  LHSPLPESFFGGNDSEENP
Sbjct: 361 SRTPVPGGGVHPLTRYVMNYIAFLSDYSGILPGIVADWPSMLHSPLPESFFGGNDSEENP 420

Query: 421 LTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIES 480
           L +RLAW+ILVLLSKLDSKAE+YHDAPLSYIFLANNLEYIVVKVRTSNL+ +LGDEWIES
Sbjct: 421 LALRLAWLILVLLSKLDSKAEVYHDAPLSYIFLANNLEYIVVKVRTSNLKSVLGDEWIES 480

Query: 481 HEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIV 540
           HEAKVKQYASSY RMGWSRVFLSLPENP AEISPERARKHFHDFN+AFEEAY+HQASWIV
Sbjct: 481 HEAKVKQYASSYIRMGWSRVFLSLPENPMAEISPERARKHFHDFNIAFEEAYKHQASWIV 540

Query: 541 TDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYLSDLLHGD 600
            D KLREHIKI+LGKKLGTLYGEFYI YR+RLENLYG DSEVRFAPDDLGNYLSDLLH D
Sbjct: 541 MDSKLREHIKITLGKKLGTLYGEFYINYRARLENLYGNDSEVRFAPDDLGNYLSDLLHAD 600

Query: 601 GNLGGSVSSSSSSSTSPYHFHGGRRSG 628
           GN GGSVSS+SS S+SPYHFHGGR SG
Sbjct: 601 GNSGGSVSSTSSLSSSPYHFHGGRHSG 627

BLAST of Lcy10g002450 vs. TAIR 10
Match: AT2G39380.1 (exocyst subunit exo70 family protein H2 )

HSP 1 Score: 526.6 bits (1355), Expect = 2.8e-149
Identity = 287/627 (45.77%), Postives = 409/627 (65.23%), Query Frame = 0

Query: 2   KNHFFKSLTPSHSPARSTPSHSPARTTP-SHGFCDSLMEENIEVAETLITKWDYAASSYA 61
           K+  F + +P  S   S PS +    +P +  F  S+MEE +E AE++I KWD  + SY 
Sbjct: 6   KSVHFSTSSPK-SFLSSFPSFTSLPASPLNQTFSQSMMEETVEAAESIIKKWDPNSPSYT 65

Query: 62  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 121
            I  LF   R EAK++I+ ++DL+ AM     +HS S  LV AQ+LMQ AM RL++EF +
Sbjct: 66  KIISLFSHSRREAKEFIRCIRDLRRAMHFLISQHSKSAKLVLAQHLMQIAMARLEKEFFQ 125

Query: 122 ILTENRIHLDPESISNRSSRDSVFTGNSDVE-------DESEDDLRFANENVPEEERMSR 181
           IL+ NR  LDPES+S  SS     + NS+ E       DE ED+L+ A E + + E+ + 
Sbjct: 126 ILSSNRDQLDPESVSGHSS----ISSNSEFEDVMQSSDDEEEDELKKAGETITKVEKAAA 185

Query: 182 SAIADLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLE 241
             ++DLK+IAE MIS GYGKEC K Y + RKSIV+EGL+ LG+ K      +RM+W+VLE
Sbjct: 186 LVMSDLKVIAETMISCGYGKECIKSYKLIRKSIVDEGLHLLGIEKCKISRFNRMDWDVLE 245

Query: 242 VKIKNWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVK 301
             IKNW+ A KI V T    E+ LCD VFS S++IRES F EI  E+ + LF FPE+  +
Sbjct: 246 HMIKNWIKAAKIGVITLLRGEKLLCDHVFSASSTIRESCFYEIVNEAGINLFKFPELVAE 305

Query: 302 SKKTPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFE 361
            K +PE+IF ++DLY AI DL P+IE +F  +S ++V++++  SL K+ +SI + L++FE
Sbjct: 306 KKPSPERIFRLMDLYAAISDLRPDIELIFHFDSVAAVKTLVLSSLKKLKDSIYTSLMEFE 365

Query: 362 AHIHKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGG 421
           + I KDSSK     GGIH LTR  M++I+ LS+YS +L  I+A+ PL  ++ + ES+F  
Sbjct: 366 STIQKDSSKALTAGGGIHKLTRSTMSFISSLSEYSRVLSEILAEHPLKKNTRMLESYFTA 425

Query: 422 ----NDSEENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNL 481
               ++   + +++ LAW+IL+ L KLD KAE Y D  LSY+FL NN++++V  VR+++L
Sbjct: 426 PILEDEHNNHAVSVHLAWLILIFLCKLDIKAESYKDVSLSYLFLVNNIQFVVDTVRSTHL 485

Query: 482 RFLLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFE 541
           R LLGD+W+  HEAK++ YA++Y+   W+ V++SLPE  ++ +SPE A+ HF  F+  FE
Sbjct: 486 RNLLGDDWLTKHEAKLRSYAANYEIAAWANVYISLPEKTSSRLSPEEAKTHFKRFHAVFE 545

Query: 542 EAYRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDL 601
           EAY  Q+S ++TD KLR  +K+S+ KK+   Y EFY KY   L     ++  V F PD+L
Sbjct: 546 EAYMKQSSCVITDAKLRNELKVSIAKKIVPEYREFYGKYLPTLSKERNIEMLVSFKPDNL 605

Query: 602 GNYLSDLLHGDGNLGGSVSSSSSSSTS 617
            NYLSDL HG   L GS SSSSS S+S
Sbjct: 606 ENYLSDLFHGTPILSGSSSSSSSLSSS 627

BLAST of Lcy10g002450 vs. TAIR 10
Match: AT5G59730.1 (exocyst subunit exo70 family protein H7 )

HSP 1 Score: 518.1 bits (1333), Expect = 1.0e-146
Identity = 290/645 (44.96%), Postives = 406/645 (62.95%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M  H F+S  P            P + T      +S ++E +    T+I+KW   A+S +
Sbjct: 1   MGKHLFRSSPP------------PEKPTAHLSLAESAVQECMSNINTVISKWTSPAASSS 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           D      + R EA++++ AV+ LQ+ M      + +S+ L+ AQNLMQ+AMK L+ EFHR
Sbjct: 61  DDFLFSTNSRREAEEFVTAVRHLQSTMHRLVSVNPSSEKLIYAQNLMQSAMKLLESEFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMS---RSAIA 180
           +L  NR +LDPE +S RS R S F+ ++       +D     EN  EE R S     A+ 
Sbjct: 121 VLKANREYLDPECVSVRSYRSSRFSTSTTTSVSDSEDESSYEENADEEHRFSGGDSDAMD 180

Query: 181 DLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIK 240
           DLK+IA+ MIS GY KEC ++Y   RKSIV+E L+NL + + + H V +M+WE+LE KIK
Sbjct: 181 DLKMIADCMISTGYAKECVRVYKTVRKSIVDETLHNLQMERFNLHQVQKMDWEILESKIK 240

Query: 241 NWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKK- 300
            WL AVK+AV+  F  ER L D VFS S  I ES F+EIT+E AL LF FPE A K KK 
Sbjct: 241 TWLKAVKLAVRKLFFGERILADHVFSSSGLIVESSFTEITQEGALILFTFPEYASKIKKL 300

Query: 301 TPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHI 360
           TPEK+F  LD+YEA+ +L  EIES+F  ES ++VRS + +SL ++ ++ R M+ DFE+ I
Sbjct: 301 TPEKMFRFLDMYEALANLYVEIESIFYFESAAAVRSQVINSLARLGDATRLMMTDFESAI 360

Query: 361 HKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFF--GGN 420
            K++SKTP+  GG+HPLTRYVMNY++FL+DYS  +  I  +W L++ +PLP+S +  GG+
Sbjct: 361 QKETSKTPIIGGGVHPLTRYVMNYLSFLADYSDSIAAIFENWKLSVPTPLPDSLYISGGD 420

Query: 421 DSE-----ENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNL 480
           ++       +P+++R+AW+IL+ L K+D KA+ Y D  LSY+FLANNL+Y+VVKVR+S L
Sbjct: 421 EANPEDLYSSPVSVRIAWVILLTLCKIDGKAQPYKDVALSYLFLANNLQYVVVKVRSSTL 480

Query: 481 RFLLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFE 540
           + LLGD+W+  HE KVK YA  ++++ W +V   LPE PT EISPE A+     FN  FE
Sbjct: 481 KVLLGDDWVFRHEEKVKLYADKFEKLAWGKVLDLLPEIPTDEISPEEAKVLVARFNDEFE 540

Query: 541 EAYRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDL 600
            +YR Q SW++ DPKLR+ IKI+L +KL  +  EFY   R     +   ++  R+ P+D+
Sbjct: 541 TSYRKQTSWVIPDPKLRDQIKITLSQKLMLVCTEFYRMNRFAYGMVGDNEAISRYTPEDI 600

Query: 601 GNYLSDLLHGDGNLG--------GSVSSSSSSSTSPYHFHGGRRS 627
           GNYLSDL  G    G        GS S S + S+S     GGR S
Sbjct: 601 GNYLSDLYFGSRGSGSVSTAGGSGSGSGSGTGSSSTGKARGGRSS 633

BLAST of Lcy10g002450 vs. TAIR 10
Match: AT5G59730.2 (exocyst subunit exo70 family protein H7 )

HSP 1 Score: 515.8 bits (1327), Expect = 5.0e-146
Identity = 284/626 (45.37%), Postives = 401/626 (64.06%), Query Frame = 0

Query: 1   MKNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYA 60
           M  H F+S  P            P + T      +S ++E +    T+I+KW   A+S +
Sbjct: 1   MGKHLFRSSPP------------PEKPTAHLSLAESAVQECMSNINTVISKWTSPAASSS 60

Query: 61  DITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHR 120
           D      + R EA++++ AV+ LQ+ M      + +S+ L+ AQNLMQ+AMK L+ EFHR
Sbjct: 61  DDFLFSTNSRREAEEFVTAVRHLQSTMHRLVSVNPSSEKLIYAQNLMQSAMKLLESEFHR 120

Query: 121 ILTENRIHLDPESISNRSSRDSVFTGNSDVEDESEDDLRFANENVPEEERMS---RSAIA 180
           +L  NR +LDPE +S RS R S F+ ++       +D     EN  EE R S     A+ 
Sbjct: 121 VLKANREYLDPECVSVRSYRSSRFSTSTTTSVSDSEDESSYEENADEEHRFSGGDSDAMD 180

Query: 181 DLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIK 240
           DLK+IA+ MIS GY KEC ++Y   RKSIV+E L+NL + + + H V +M+WE+LE KIK
Sbjct: 181 DLKMIADCMISTGYAKECVRVYKTVRKSIVDETLHNLQMERFNLHQVQKMDWEILESKIK 240

Query: 241 NWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKK- 300
            WL AVK+AV+  F  ER L D VFS S  I ES F+EIT+E AL LF FPE A K KK 
Sbjct: 241 TWLKAVKLAVRKLFFGERILADHVFSSSGLIVESSFTEITQEGALILFTFPEYASKIKKL 300

Query: 301 TPEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHI 360
           TPEK+F  LD+YEA+ +L  EIES+F  ES ++VRS + +SL ++ ++ R M+ DFE+ I
Sbjct: 301 TPEKMFRFLDMYEALANLYVEIESIFYFESAAAVRSQVINSLARLGDATRLMMTDFESAI 360

Query: 361 HKDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFF--GGN 420
            K++SKTP+  GG+HPLTRYVMNY++FL+DYS  +  I  +W L++ +PLP+S +  GG+
Sbjct: 361 QKETSKTPIIGGGVHPLTRYVMNYLSFLADYSDSIAAIFENWKLSVPTPLPDSLYISGGD 420

Query: 421 DSE-----ENPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNL 480
           ++       +P+++R+AW+IL+ L K+D KA+ Y D  LSY+FLANNL+Y+VVKVR+S L
Sbjct: 421 EANPEDLYSSPVSVRIAWVILLTLCKIDGKAQPYKDVALSYLFLANNLQYVVVKVRSSTL 480

Query: 481 RFLLGDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFE 540
           + LLGD+W+  HE KVK YA  ++++ W +V   LPE PT EISPE A+     FN  FE
Sbjct: 481 KVLLGDDWVFRHEEKVKLYADKFEKLAWGKVLDLLPEIPTDEISPEEAKVLVARFNDEFE 540

Query: 541 EAYRHQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDL 600
            +YR Q SW++ DPKLR+ IKI+L +KL  +  EFY   R     +   ++  R+ P+D+
Sbjct: 541 TSYRKQTSWVIPDPKLRDQIKITLSQKLMLVCTEFYRMNRFAYGMVGDNEAISRYTPEDI 600

Query: 601 GNYLSDLLHGDGNLGGSVSSSSSSST 616
           GNYLSDL  G     GSVS++  S +
Sbjct: 601 GNYLSDLYFGSRG-SGSVSTAGGSGS 613

BLAST of Lcy10g002450 vs. TAIR 10
Match: AT3G55150.1 (exocyst subunit exo70 family protein H1 )

HSP 1 Score: 506.9 bits (1304), Expect = 2.3e-143
Identity = 292/623 (46.87%), Postives = 391/623 (62.76%), Query Frame = 0

Query: 2   KNHFFKSLTPSHSPARSTPSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYAD 61
           K  FF S +P + P+  +   S   +  +  F  ++MEE +E A+++I KWD    S+  
Sbjct: 6   KFSFFSSSSPKYHPSSPSSFTSFPASPLNQNFTQAMMEETVETADSVIRKWDPNTPSFTK 65

Query: 62  ITPLFQDDRYEAKQYIKAVKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHRI 121
           I  LF   R EAK++I+ V+DL+ AM     + S S  L  AQ LMQ AM RL++EF +I
Sbjct: 66  IVSLFNHSRKEAKEFIRCVRDLRRAMHFLVSQDSQSPKLALAQTLMQIAMARLEKEFFQI 125

Query: 122 LTENRIHLDPESISNRS--SRDSVFTG--NSDVEDESEDDLRFANENVPEEERMSRSAIA 181
           L+ NR  LDPES+S +S  S +S F     SD +DES++++R A E++ + E+ S   ++
Sbjct: 126 LSSNRDKLDPESVSGQSSISSNSEFEDVMGSDDDDESDNEMRKAGESITQVEKASAMVMS 185

Query: 182 DLKLIAEGMISVGYGKECGKIYTVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIK 241
           DLK IAE MIS GYGKEC KIY   RKSIV+EGL  LG+        HR +W  LE  IK
Sbjct: 186 DLKAIAESMISCGYGKECVKIYKRVRKSIVDEGLSLLGIEIYKGSRFHRTDWVTLEHMIK 245

Query: 242 NWLNAVKIAVKTFFEAERFLCDQVFSISASIRESVFSEITKESALTLFGFPEMAVKSKKT 301
           NW+ A KI + T F  E+ LCD VFS S S RES F EI  E+A  LF FPE   K KK+
Sbjct: 246 NWIKAAKIGIATLFRGEKLLCDHVFSASNSTRESCFYEIANEAATNLFKFPEFVAKEKKS 305

Query: 302 PEKIFLILDLYEAIFDLLPEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIH 361
            E+IF ++DL  AI DL  +IE +F  ++ + V+S    SL K+  SI S L DFE+ I 
Sbjct: 306 HERIFPLMDLQAAISDLWQDIEMIFHFDAVAGVKSQALTSLQKLKVSIHSALTDFESIIQ 365

Query: 362 KDSSKTPVPSGGIHPLTRYVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSE 421
           KD++K   P GGIH LTR  MN+I+ LS YSG+L  I+AD PL  ++ L ES+     SE
Sbjct: 366 KDTTKALTPGGGIHKLTRSTMNFISSLSKYSGVLSEILADHPLPRNTRLLESYVRAPISE 425

Query: 422 E----NPLTIRLAWMILVLLSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLL 481
           +    + L++  AW+ILVLL KLD+KAE Y D  LSY+FLANNL+ I+  V ++ LR LL
Sbjct: 426 DEQHNHALSVHFAWLILVLLCKLDTKAEHYKDVSLSYLFLANNLQIIIETVGSTPLRNLL 485

Query: 482 GDEWIESHEAKVKQYASSYQRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYR 541
           GD+W+  HE K+  YA +Y+   WS VF+SLPE PT ++SPE A+ +F  F+ AFEEAY 
Sbjct: 486 GDDWLNKHEDKLCAYAGNYEIAAWSNVFMSLPEEPT-DLSPEEAKIYFRRFHTAFEEAYM 545

Query: 542 HQASWIVTDPKLREHIKISLGKKLGTLYGEFYIKYRSRLENLYGLDSEVRFAPDDLGNYL 601
            Q+S +V + KLR+ +K+S+ KKL   Y EFY KY   L     ++  VRF PD+L NY+
Sbjct: 546 KQSSRVVPNAKLRDELKVSIAKKLVPEYREFYRKYLPMLGQERNIEILVRFKPDNLENYI 605

Query: 602 SDLLHGDGNLGGSVSSSSSSSTS 617
           SDL HG      S  SSSSSS+S
Sbjct: 606 SDLFHGTPIHASSSFSSSSSSSS 627

BLAST of Lcy10g002450 vs. TAIR 10
Match: AT3G09520.1 (exocyst subunit exo70 family protein H4 )

HSP 1 Score: 500.7 bits (1288), Expect = 1.7e-141
Identity = 277/608 (45.56%), Postives = 392/608 (64.47%), Query Frame = 0

Query: 20  PSHSPARTTPSHGFCDSLMEENIEVAETLITKWDYAASSYADITPLFQDDRYEAKQYIKA 79
           P   P   TP      + +   IE A  +I +W+   +++A +T +F +++ EA  +I+ 
Sbjct: 21  PPPPPLTLTPRQSLESAAIYGVIESAADIIERWNTETNTFAKVTCMFYENKPEAMMFIER 80

Query: 80  VKDLQTAMQHFGFEHSNSQHLVRAQNLMQTAMKRLQREFHRILTENRIHLDPESISNRSS 139
           VKDLQ  M     E  NS+ L+R+  LMQ AMKRLQ+EF++IL+ NR +LDPES+S RSS
Sbjct: 81  VKDLQKTMDVLVSEDPNSERLLRSHKLMQIAMKRLQKEFYQILSMNRAYLDPESVSTRSS 140

Query: 140 RDSVFTGNSDVEDESEDDLRFANENVPEEERMSRSAIADLKLIAEGMISVGYGKECGKIY 199
             S  +  SD  D  ED      + + E E +S + + DLK IAE MI  GY KEC  IY
Sbjct: 141 LTSARSSYSDFPDYVED-----LDTIIELEEVSTNVMTDLKSIAECMIGSGYAKECLSIY 200

Query: 200 TVSRKSIVEEGLYNLGVAKTSYHHVHRMEWEVLEVKIKNWLNAVKIAVKTFFEAERFLCD 259
              RKSI++EG+Y L V KTS   V +M WEV+E+KI++WL AVK++++T F+ E+ LCD
Sbjct: 201 KSIRKSIIDEGIYRLEVEKTSTGKVKKMSWEVMELKIRSWLKAVKVSMETLFKGEKILCD 260

Query: 260 QVFSISASIRESVFSEITKESALTLFGFPEM----AVKSKKTPEKIFLILDLYEAIFDLL 319
            VF  S +IRES FS+I+++ AL LFGFPE+      K    PEK+F +LD+Y AI    
Sbjct: 261 HVFESSDAIRESCFSDISRDGALLLFGFPEIINTKTSKKHSPPEKVFRLLDMYTAIAGNW 320

Query: 320 PEIESMFSCESTSSVRSVIDHSLTKIAESIRSMLVDFEAHIHKDSSKTPVPSGGIHPLTR 379
             IES+FS +S S VRS+   SL  ++ESIRS+LV+FE+ I KDSSK  VP GG+HPLT 
Sbjct: 321 QAIESIFSFDSISVVRSLALKSLISLSESIRSLLVEFESGIQKDSSKVVVPGGGVHPLTI 380

Query: 380 YVMNYIAFLSDYSGILPGIVADWPLTLHSPLPESFFGGNDSEENP---LTIRLAWMILVL 439
            VM++++ L+DYS +L  I+A  P    S LPES+F  ++S+++P   LTIR AW+ILVL
Sbjct: 381 SVMDHLSLLADYSNVLVDILAGSPPPDRSLLPESYFNVSESDDSPSSELTIRFAWIILVL 440

Query: 440 LSKLDSKAELYHDAPLSYIFLANNLEYIVVKVRTSNLRFLLGDEWIESHEAKVKQYASSY 499
           L K+D K+  Y D  + Y+FL NNL+++V + R+SNL+ LLG++WI  H AK++Q+A SY
Sbjct: 441 LCKIDRKSIHYKDFSIQYLFLTNNLQHVVSRARSSNLKNLLGEDWITRHFAKMRQFAGSY 500

Query: 500 QRMGWSRVFLSLPENPTAEISPERARKHFHDFNMAFEEAYRHQASWIVTDPKLREHIKIS 559
           +R+ W  V  +LPEN T E++PE  ++ F  F+ +FE AY   +  +V DP +R+ IK+S
Sbjct: 501 KRLAWGPVVATLPENRTVEMTPEEVKERFEKFSESFENAYSKHSVCVVADPNIRDEIKVS 560

Query: 560 LGKKLGTLYGEFYIKYRSRL----ENLYGLDSEVRFAPDDLGNYLSDLLHGDGNLGGSVS 617
           + +KL  +Y EFY    S +    +    L+S VRF P+D+ NYLSDL    G+ G S +
Sbjct: 561 ISRKLVPIYREFYNTRGSVILGEGDGARNLNSVVRFTPEDIENYLSDLFREKGSSGNSSA 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VY273.3e-14246.87Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=... [more]
Q9FGH92.5e-7331.33Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=... [more]
Q9LZD36.0e-7228.89Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=... [more]
Q9LMJ42.8e-6129.27Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=... [more]
F4KG581.8e-6030.35Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1G9740.0e+0089.31Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114520... [more]
A0A6J1HIW80.0e+0089.47Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114648... [more]
A0A6J1I2W50.0e+0089.47Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111469425... [more]
A0A6J1KHD60.0e+0088.52Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111493246... [more]
A0A0A0LXW30.0e+0089.01Exocyst subunit Exo70 family protein OS=Cucumis sativus OX=3659 GN=Csa_1G481200 ... [more]
Match NameE-valueIdentityDescription
KAG6607205.10.0e+0089.31Exocyst complex component EXO70H1, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6583465.10.0e+0089.63Exocyst complex component EXO70H1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022948393.10.0e+0089.31exocyst complex component EXO70H1-like [Cucurbita moschata][more]
XP_022964887.10.0e+0089.47exocyst complex component EXO70H1-like [Cucurbita moschata][more]
XP_023519812.10.0e+0089.47exocyst complex component EXO70H1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G39380.12.8e-14945.77exocyst subunit exo70 family protein H2 [more]
AT5G59730.11.0e-14644.96exocyst subunit exo70 family protein H7 [more]
AT5G59730.25.0e-14645.37exocyst subunit exo70 family protein H7 [more]
AT3G55150.12.3e-14346.87exocyst subunit exo70 family protein H1 [more]
AT3G09520.11.7e-14145.56exocyst subunit exo70 family protein H4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004140Exocyst complex component Exo70PFAMPF03081Exo70coord: 237..595
e-value: 1.5E-104
score: 350.0
IPR004140Exocyst complex component Exo70PANTHERPTHR12542EXOCYST COMPLEX PROTEIN EXO70coord: 10..607
NoneNo IPR availableGENE3D1.20.1280.170Exocyst complex component Exo70coord: 48..602
e-value: 5.1E-160
score: 535.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 604..621
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 604..627
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availablePANTHERPTHR12542:SF141EXOCYST SUBUNIT EXO70 FAMILY PROTEINcoord: 10..607
IPR016159Cullin repeat-like-containing domain superfamilySUPERFAMILY74788Cullin repeat-likecoord: 60..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy10g002450.1Lcy10g002450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006887 exocytosis
biological_process GO:0015031 protein transport
cellular_component GO:0000145 exocyst