Lcy10g000790 (gene) Sponge gourd (P93075) v1

BLAST of Lcy10g000790 vs. ExPASy Swiss-Prot
Match: Q39033 (Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.0e-166
Identity = 312/587 (53.15%), Postives = 393/587 (66.95%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           M +  ++VCFCF RRF     + P ++K +F++YSENG M ++ L RFL ++Q +    R
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLN--LALLPPHQAYQDMGAPLSHYYIF 120
             AQ+I  +    +     GL L+ FF+YL GD N  LAL   H+ + DM AP+SHY+IF
Sbjct: 61  EDAQSIINSAS--SLLHRNGLHLDAFFKYLFGDNNPPLAL---HKVHHDMDAPISHYFIF 120

Query: 121 TGHNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVK 180
           TGHNSYLTGNQL S  S  PII AL++GVRVIELD+WPNS K+DI VLHG   TLT  V 
Sbjct: 121 TGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHG--MTLTTPVG 180

Query: 181 LIKCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPK-SEDSNEF 240
           LIKCL+AIR HAF+ SDYPVV+T EDHLT DL+ KVA+MVT  FG++L+ P   E   EF
Sbjct: 181 LIKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEF 240

Query: 241 PSPESLKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKP------------ 300
           PSP SLKR+I+ISTKPP+  K    K      K +D  D+++W    P            
Sbjct: 241 PSPNSLKRRIIISTKPPKEYK--EGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKD 300

Query: 301 ----QDVDEDHLEEDKDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEE 360
                D D+D  +EDK +  A P+Y+ LIAIH GK K G   +    D +KV RLSLSEE
Sbjct: 301 DLDGNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPK-GGITECLKVDPDKVRRLSLSEE 360

Query: 361 ELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYL 420
           +L+ A + +  +I+ FTQ NLLR+YPKG+R  SSNYNP++ W+HGAQMVAFNMQGYGR L
Sbjct: 361 QLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSL 420

Query: 421 WIMEGMFRGNGGCGYIKKPDFLLNNPNSKS--TPSTT---ENCLKVKVYMGVGWHLEFGL 480
           W+M+GMFR NGGCGYIKKPD LL + +      P  T   +  L+V VYMG GW+ +F  
Sbjct: 421 WLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRH 480

Query: 481 SHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVV 540
           +HFD YSPPDFY ++GI G P D   KKT T++D WIP WDE F F +  PELALL++ V
Sbjct: 481 THFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEV 540

Query: 541 RDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMR 562
            +YD S  D F GQT LPV EL  GIRA PL++RKGEKYK VKLL++
Sbjct: 541 HEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVK 577

BLAST of Lcy10g000790 vs. ExPASy Swiss-Prot
Match: Q39032 (Phosphoinositide phospholipase C 1 OS=Arabidopsis thaliana OX=3702 GN=PLC1 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 8.0e-164
Identity = 297/582 (51.03%), Postives = 395/582 (67.87%), Query Frame = 0

Query: 3   EHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGE-KSDMR- 62
           + +F+VCFC  R F  K  +PPE++KN+F +YS++  M+ +++ RF+ ++QGE  +D+  
Sbjct: 2   KESFKVCFCCVRNFKVKSSEPPEEIKNLFHDYSQDDRMSADEMLRFVIQVQGETHADINY 61

Query: 63  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 122
            + IF  LKH   F  RG+ LE F++YLL D N  L    + +QDM  PLSHY+++TGHN
Sbjct: 62  VKDIFHRLKHHGVFHPRGIHLEGFYRYLLSDFNSPLPLTREVWQDMNQPLSHYFLYTGHN 121

Query: 123 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 182
           SYLTGNQL S SS+ PI+KAL  GVRVIELDLWPNS   + +V HGG  TLT+   L KC
Sbjct: 122 SYLTGNQLNSNSSIEPIVKALRNGVRVIELDLWPNSSGKEAEVRHGG--TLTSREDLQKC 181

Query: 183 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 242
           L  ++++AF  S YPVV+T EDHLT  L++KVAKMV+ TFG  L+    E +  FPSPES
Sbjct: 182 LNVVKENAFQVSAYPVVLTLEDHLTPILQKKVAKMVSKTFGGSLFQCTDETTECFPSPES 241

Query: 243 LKRKILISTKPPEH------NKGESTKKKPSADKQRDTSDDDIWVSAKPQDVDEDHLEED 302
           LK KILISTKPP+       +KG +T +   A K  D                E+ ++E+
Sbjct: 242 LKNKILISTKPPKEYLQTQISKGSTTDESTRAKKISDA---------------EEQVQEE 301

Query: 303 KDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFN-DTEKVSRLSLSEEELKIATKNHGDEII 362
            +E +A+ EYR LI+IH G  K G  L+   N D  +V RLS+SE+ L+   K  G +++
Sbjct: 302 DEESVAI-EYRDLISIHAGNRKGG--LKNCLNGDPNRVIRLSMSEQWLETLAKTRGPDLV 361

Query: 363 SFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCG 422
            FTQRNLLR++PK +RFDSSNY+P++ W HGAQMVAFNMQ +GRYLW+M+GMF+ NGGCG
Sbjct: 362 KFTQRNLLRIFPKTTRFDSSNYDPLVGWIHGAQMVAFNMQSHGRYLWMMQGMFKANGGCG 421

Query: 423 YIKKPDFLLNN--------PNSKSTPSTTENCLKVKVYMGVGWHLEFGLSHFDFYSPPDF 482
           Y+KKPD LL+N        P S++ P  T   LKVK+Y G GW+++F L HFD YSPPDF
Sbjct: 422 YVKKPDVLLSNGPEGEIFDPCSQNLPIKT--TLKVKIYTGEGWNMDFPLDHFDRYSPPDF 481

Query: 483 YVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKF 542
           Y K+GI G P D A  +T   KD+W P+WD+EF F +R PEL+LL + V+DYD +  + F
Sbjct: 482 YAKVGIAGVPLDTASYRTEIDKDEWFPIWDKEFEFPLRVPELSLLCITVKDYDSNTQNDF 541

Query: 543 AGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMRFNLDER 568
           AGQT  P+ E+R GIRAV L++R GE YKHV+LLMRF L+ R
Sbjct: 542 AGQTCFPLSEVRPGIRAVRLHDRAGEVYKHVRLLMRFVLEPR 561

BLAST of Lcy10g000790 vs. ExPASy Swiss-Prot
Match: Q9LY51 (Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 3.8e-158
Identity = 304/589 (51.61%), Postives = 390/589 (66.21%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           M +  ++VCFCF RR+       P ++K +FD YS+ G M  + L RFL ++Q  K D  
Sbjct: 1   MSKQTYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGLMTTDLLLRFLIDVQ--KQDKA 60

Query: 61  AQTIFKNLKHPNT--FQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTG 120
            +   +++ + ++      GL L+ FF+YL    N + L   + +QDM APLSHY+I+TG
Sbjct: 61  TKEEAQDIVNASSSLLHRNGLHLDAFFKYLFAVTN-SPLSSLEVHQDMDAPLSHYFIYTG 120

Query: 121 HNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLI 180
           HNSYLTGNQL S  S  PII+AL++GVRVIELD+WPNS ++ I VLHG  RTLT+ V+LI
Sbjct: 121 HNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHG--RTLTSPVELI 180

Query: 181 KCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKS-EDSNEFPS 240
           KCLRAIR+HAF+ SDYPVV+T EDHLT  L+ KVA+MVT  FG+ML+ P S E   EFPS
Sbjct: 181 KCLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFPS 240

Query: 241 PESLKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKPQ------------- 300
           P  LK++I+ISTKPP+  K  +        K RD  D ++W    P              
Sbjct: 241 PAFLKKRIMISTKPPKEYK--AATDDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDKNDS 300

Query: 301 --DVDEDHLEEDKDED-------IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLS 360
             D D+D  ++D D+D        A PEY+ LIAI  GK K G   +    D +KV RLS
Sbjct: 301 NGDDDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPK-GGITECLKVDPDKVRRLS 360

Query: 361 LSEEELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGY 420
           LSEE+L+ A++ +  +I+ FTQRNLLRVYPKG+R  SSNYNP++AW+HGAQMVAFNMQG 
Sbjct: 361 LSEEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNMQGL 420

Query: 421 GRYLWIMEGMFRGNGGCGYIKKPDFLLNNPNSKSTPSTT---ENCLKVKVYMGVGWHLEF 480
           GR LW+M+GMFRGNGGCGYIKKPD LL + N+   P  T   +  L+V +YMG GW+ +F
Sbjct: 421 GRSLWVMQGMFRGNGGCGYIKKPDLLLKS-NAVFDPEATLPVKTTLRVTIYMGEGWYYDF 480

Query: 481 GLSHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKV 540
             +HFD YSPPDFY ++GI G P D   KKT T++D WIP WDE F F +  PELALL++
Sbjct: 481 PHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRI 540

Query: 541 VVRDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMR 562
            V +YD S  D F GQ  LPV ELR GIRAVPL N+ G K + VKLL+R
Sbjct: 541 EVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVR 580

BLAST of Lcy10g000790 vs. ExPASy Swiss-Prot
Match: Q8GV43 (Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 SV=2)

HSP 1 Score: 546.6 bits (1407), Expect = 3.4e-154
Identity = 297/603 (49.25%), Postives = 384/603 (63.68%), Query Frame = 0

Query: 4   HNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYS-----------------ENGTMNIEQLK 63
           +N+R+   + R+F   +  P +DV++ F +++                   G M  EQL 
Sbjct: 17  YNYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLC 76

Query: 64  RFLEEIQGEKSDMRAQ----TIFKNLKHPNTFQNRGLCLEDFFQYLL-GDLNLALLPPHQ 123
            FL++     +   AQ     + +   H   F   GL L+DFF +L   DLN  + P   
Sbjct: 77  SFLDDHGESTTVAEAQRLIDEVIRRRHHVTRFTRHGLDLDDFFNFLFYDDLNPPITP--H 136

Query: 124 AYQDMGAPLSHYYIFTGHNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDI 183
            +QDM APLSHY+I+TGHNSYLTGNQL S  S  P+IKAL+RGVRVIELDLWPNS   DI
Sbjct: 137 VHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDI 196

Query: 184 KVLHGGCRTLTASVKLIKCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFG 243
            VLHG  RTLT  V L+KCL++IRD+AF++S YPV+IT EDHLT DL+ KVA+M T  FG
Sbjct: 197 NVLHG--RTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFG 256

Query: 244 DMLYVPKSEDSNEFPSPESLKRKILISTKPP--------------EHNKGESTKKKPSAD 303
            MLY P+S+   EFPSP SL  +I+ISTKPP              ++N   S++ +    
Sbjct: 257 QMLYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRT 316

Query: 304 KQRDTSDDDIWVSAKPQDVDEDHLEEDKDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFND 363
           ++  T +  ++        D D  +E+  ED   P Y+ LI IH GK K G+  +     
Sbjct: 317 EEIQTLESMLFDQDFESKSDSDQEDEEASED-QKPAYKRLITIHAGKPK-GTVKEEMKVV 376

Query: 364 TEKVSRLSLSEEELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQM 423
            +KV RLSLSE+EL     ++  +++ FTQRNLLR+YPKG+RF+SSNY P++ WTHGAQM
Sbjct: 377 VDKVRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQM 436

Query: 424 VAFNMQGYGRYLWIMEGMFRGNGGCGYIKKPDFLLN--------NPNSKSTPSTTENCLK 483
           +AFNMQGYG+ LW+M GMFR NGGCGY+KKP+FL+         +P  K     T   LK
Sbjct: 437 IAFNMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKET---LK 496

Query: 484 VKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFS 543
           VKVYMG GW ++F  +HFD YSPPDFY K+ IVG P D A+KKT  I+D W P+WDEEFS
Sbjct: 497 VKVYMGDGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFS 556

Query: 544 FSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLL 563
           F +  PELALL++ VR+YD S  D F GQT LPV ELR GIR+VPLY++KGEK K V+LL
Sbjct: 557 FPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKMKSVRLL 610

BLAST of Lcy10g000790 vs. ExPASy Swiss-Prot
Match: Q944C1 (Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 SV=2)

HSP 1 Score: 542.3 bits (1396), Expect = 6.3e-153
Identity = 297/595 (49.92%), Postives = 388/595 (65.21%), Query Frame = 0

Query: 5   NFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSE-NGTMNIEQLKRFLEEIQG------EKS 64
           +++ C  F R+F   +  P EDV+++F++Y+E +  M+ EQL++ + E  G      E++
Sbjct: 9   SYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLEEA 68

Query: 65  DMRAQTIFKNLKHPNTFQNRGLCLEDFFQYLLG-DLNLALLPP--HQAYQDMGAPLSHYY 124
           +     + +   H   F  R L L+DF  YL   DLN    PP   Q +Q+M APLSHY+
Sbjct: 69  ERIVDEVLRRKHHIAKFTRRNLTLDDFNYYLFSTDLN----PPIADQVHQNMDAPLSHYF 128

Query: 125 IFTGHNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTAS 184
           IFTGHNSYLTGNQL S  S  PI  AL RGVRV+ELDLWP    +D+ V HG  RTLT  
Sbjct: 129 IFTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRG-TDDVCVKHG--RTLTKE 188

Query: 185 VKLIKCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNE 244
           VKL KCL +I+ +AF  S YPV+IT EDHLT  L+ KVAKM+T TFGDMLY   S+   E
Sbjct: 189 VKLGKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQE 248

Query: 245 FPSPESLKRKILISTKPP-EHNKGESTKKKPSADKQRDTSDDDIW--------------- 304
           FPSPE LK KILISTKPP E+ +   TK+K + +K +D SD+D+W               
Sbjct: 249 FPSPEELKEKILISTKPPKEYLEANDTKEKDNGEKGKD-SDEDVWGKEPEDLISTQSDLD 308

Query: 305 -VSAKPQDVDED-----HLEEDKDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVS 364
            V++   D+++D       E D    +  PEY+ LIAIH GK K G  +     D  K+ 
Sbjct: 309 KVTSSVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRM-ALKVDPNKIR 368

Query: 365 RLSLSEEELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNM 424
           RLSLSE+ L+ A  ++G ++I FTQ+N LR+YPKG+RF+SSNY P + W  GAQM+AFNM
Sbjct: 369 RLSLSEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNM 428

Query: 425 QGYGRYLWIMEGMFRGNGGCGYIKKPDFLLN-NPNSK----STPSTTENCLKVKVYMGVG 484
           QGYGR LW+MEGMFR NGGCGY+KKPDFL++ +PN +       S+ +  LKVKV MG G
Sbjct: 429 QGYGRALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDG 488

Query: 485 WHLEFGLSHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPEL 544
           W L+F  +HFD YSPPDF+V++GI G P D+  +KT    D W P+W++EF+F +  PEL
Sbjct: 489 WLLDFKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPEL 548

Query: 545 ALLKVVVRDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMRF 563
           ALL+V V ++D +  D F GQT LPV E+R GIRAVPL+NRKG KY   +LLMRF
Sbjct: 549 ALLRVEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRF 594

BLAST of Lcy10g000790 vs. ExPASy TrEMBL
Match: A0A6J1HMF1 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111464903 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 6.2e-252
Identity = 442/572 (77.27%), Postives = 486/572 (84.97%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+H+FRVCFCF RRF T   + PEDVK +FD+YSENGTMNI+QL+ FLEE+QGE+S+M+
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A  IF NLKH N FQ RGL LEDFFQYLLGDLNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFQYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+KN I VLHGG  T TA VKLIKC
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGG--TFTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVT TFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHLEE-DKDED 300
           LK +ILISTKPPEH K ESTK+KPSADKQRDT+DDDIW SA   +D DED+LEE DKDE+
Sbjct: 241 LKGRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTDEDYLEEDDKDEE 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGSNLQTFFND EKVSRLSLSE+EL+ AT N G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKGSR DSSNYNPMLAWTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYIKKP
Sbjct: 361 NLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKKP 420

Query: 421 DFLLNNPNSKSTPSTTENC-----LKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLLNN   +++ S + +      LK+KVYMG GW LEFG SHFDFYSPPD YVKI IVG
Sbjct: 421 DFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGHSHFDFYSPPDLYVKIRIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   K+T  I+DQW+PVW+EEFSFSI APELALL+VVV D+D SG D FAGQT LPV
Sbjct: 481 VREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           RELRSGIRAVPLYNRKGE YKHVKLLMRF  +
Sbjct: 541 RELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. ExPASy TrEMBL
Match: A0A6J1I4E1 (Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111469541 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.2e-250
Identity = 439/572 (76.75%), Postives = 486/572 (84.97%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+H+FRVCFCF RRF T   + PEDVK +FD+YSENGTMNI+QL+ FLEE+QGE+S+M+
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A  IF NLKH N FQ RGL LEDFF+YLLG+LNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFRYLLGELNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+KN I VLHGG  TLTA VKLIKC
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGINVLHGG--TLTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVT TFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHLEE-DKDED 300
           LK +ILISTKPPEH K ESTK+KPSADK+RDT+DDDIW S    +D DED+LEE DKDE+
Sbjct: 241 LKGRILISTKPPEHTKVESTKEKPSADKRRDTADDDIWESTMSREDTDEDYLEEDDKDEE 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGSNLQTFFND EKVSRLSLSE+EL+ AT N G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKGSR DSSNYNPMLAWTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYIKKP
Sbjct: 361 NLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKKP 420

Query: 421 DFLLNNPNSKSTPSTTENC-----LKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLLNN   +++ S + +      LK+KVYMG GW LEFG SHFDFYSPPD YVKI IVG
Sbjct: 421 DFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGPSHFDFYSPPDLYVKIRIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   K+T  I+DQW+PVW+EEFSFSI APELALL+VVV D+D SG D FAGQT LPV
Sbjct: 481 VREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           RELRSGIRAVPLYNRKGE YKHVKLLMRF  +
Sbjct: 541 RELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. ExPASy TrEMBL
Match: A0A6J1D9W9 (Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111018981 PE=4 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.7e-249
Identity = 434/575 (75.48%), Postives = 481/575 (83.65%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+ +FRVCFCF RRF T   + PEDV+ MF +YSENG MNI+ L+RFLEEIQGE SD +
Sbjct: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           AQ IF NLKH   FQ RGL LEDFF+YL GDLNLA  P    YQDMGAPLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL RGVR IELDLWPNS+KNDI VLHGGCRTLTA VKLIKC
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGCRTLTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YP+VITFEDHLT DLR++VA+MVT TFGDMLYVP+SE  N FPSPES
Sbjct: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHL----EEDK 300
           L+R+ILISTKPPEH KGES K+KPSADKQRDT+DDDIW S  + QD+DEDHL    EEDK
Sbjct: 241 LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK 300

Query: 301 DEDIAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISF 360
           DE+ AVPEYRSLIAIH GKMK GS+L+  FND EKVSRLSLSE+EL+ A + HG +II F
Sbjct: 301 DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF 360

Query: 361 TQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYI 420
           T+RNLLRVYPKG R DSSNYNPML WTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYI
Sbjct: 361 TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI 420

Query: 421 KKPDFLLNNPN-----SKSTPSTTENCLKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIG 480
           KKPD LL N +     S+ST S   + LKVKVYMG GWHLEFGLSHFDFYSPPD YVK+G
Sbjct: 421 KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG 480

Query: 481 IVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTF 540
           IVG  +D   +KT+ I+D+WIPVW+EEF FSI APELALL VVVRDYD SG D FAGQT 
Sbjct: 481 IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC 540

Query: 541 LPVRELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           LPV ELRSGIR+VPLY+RKGEK+KHVKLLMRF  +
Sbjct: 541 LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 575

BLAST of Lcy10g000790 vs. ExPASy TrEMBL
Match: A0A6J1DD40 (Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111018981 PE=4 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 5.1e-246
Identity = 432/575 (75.13%), Postives = 479/575 (83.30%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+ +FRVCFCF RRF T   + PEDV+ MF +YSENG MNI+ L+RFLEEIQGE SD +
Sbjct: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           AQ IF NLKH   FQ RGL LEDFF+YL GDLNLA  P    YQDMGAPLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL RGVR IELDLWPNS+KNDI VLHGG  TLTA VKLIKC
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGG--TLTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YP+VITFEDHLT DLR++VA+MVT TFGDMLYVP+SE  N FPSPES
Sbjct: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHL----EEDK 300
           L+R+ILISTKPPEH KGES K+KPSADKQRDT+DDDIW S  + QD+DEDHL    EEDK
Sbjct: 241 LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK 300

Query: 301 DEDIAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISF 360
           DE+ AVPEYRSLIAIH GKMK GS+L+  FND EKVSRLSLSE+EL+ A + HG +II F
Sbjct: 301 DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF 360

Query: 361 TQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYI 420
           T+RNLLRVYPKG R DSSNYNPML WTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYI
Sbjct: 361 TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI 420

Query: 421 KKPDFLLNNPN-----SKSTPSTTENCLKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIG 480
           KKPD LL N +     S+ST S   + LKVKVYMG GWHLEFGLSHFDFYSPPD YVK+G
Sbjct: 421 KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG 480

Query: 481 IVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTF 540
           IVG  +D   +KT+ I+D+WIPVW+EEF FSI APELALL VVVRDYD SG D FAGQT 
Sbjct: 481 IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC 540

Query: 541 LPVRELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           LPV ELRSGIR+VPLY+RKGEK+KHVKLLMRF  +
Sbjct: 541 LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573

BLAST of Lcy10g000790 vs. ExPASy TrEMBL
Match: A0A6J1GAW1 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111452350 PE=4 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 3.4e-234
Identity = 406/571 (71.10%), Postives = 471/571 (82.49%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           M +H+FRVCF F RRF T   + PEDVK MF++YS++GTMNI+QL+ FLEEIQGE S+ +
Sbjct: 1   MSKHSFRVCFFFSRRFRTNAAEAPEDVKMMFNKYSKHGTMNIDQLRTFLEEIQGETSETK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A+ IF +LKH N FQ RGL LEDFF+YLLGDLNLA       YQDMGAPLSHYYIFTGHN
Sbjct: 61  AEDIFNSLKHLNIFQRRGLYLEDFFRYLLGDLNLAFPHCQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL+S+SSVTPII+AL+RGVR IELDLWP S+ N I+V HG  RTLT+ V LIKC
Sbjct: 121 SYLTGNQLYSKSSVTPIIRALKRGVRAIELDLWPTSKNNGIEVRHG--RTLTSPVSLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHL  +LR++VAKMV  TF DMLYVP+ ED N+  SPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLNPELRKEVAKMVNETFEDMLYVPELEDLNKLRSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKPQ-DVDEDHLEEDKDEDI 300
           LK+++LIS KPP++ KGESTK+KPS DKQRD +DDDIW S +PQ DVDED LEED+DED+
Sbjct: 241 LKKRVLISAKPPKNKKGESTKEKPSTDKQRDIADDDIWGSVRPQEDVDEDDLEEDEDEDV 300

Query: 301 AVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQRN 360
           AVPEYR LIAIH  K K G NLQ F NDTE VSR SLSE+ L+ ATK++G +II FTQRN
Sbjct: 301 AVPEYRKLIAIHAKKKKPGLNLQAFLNDTESVSRFSLSEQALEKATKDYGRDIIRFTQRN 360

Query: 361 LLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKPD 420
           LLRVYPKG R DSSNYNPML WTHGAQMVAFNMQGYG+YLWIM+GMFRGNGGCGYIKKPD
Sbjct: 361 LLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMQGMFRGNGGCGYIKKPD 420

Query: 421 FLLNN-----PNSKSTPSTTENCLKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVGD 480
           FLLNN      NS+S+ ST    L +KVYMG GW+ EFGLSHFDFYSPPD YV+IGIVG 
Sbjct: 421 FLLNNLDHKPSNSRSSSSTMIRRLTIKVYMGEGWNSEFGLSHFDFYSPPDLYVQIGIVGV 480

Query: 481 PEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPVR 540
            +D   ++T TI+DQW+PVW+ +FSFSI  PELALL+V+VRD+D SG D FAGQT LPV+
Sbjct: 481 RDDTTTERTATIQDQWVPVWNAKFSFSISTPELALLRVIVRDHDTSGKDDFAGQTCLPVK 540

Query: 541 ELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           ELRSGIRAVPLY+++GEKYKHVKLLM F  +
Sbjct: 541 ELRSGIRAVPLYSKRGEKYKHVKLLMSFEFE 569

BLAST of Lcy10g000790 vs. NCBI nr
Match: XP_038894759.1 (phosphoinositide phospholipase C 2-like [Benincasa hispida])

HSP 1 Score: 892.5 bits (2305), Expect = 1.9e-255
Identity = 444/572 (77.62%), Postives = 490/572 (85.66%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+H+FRVCFCF RRF T   + PEDVK MF++YSENG MNI+QL+ FLEEIQGE+SD++
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKMMFNQYSENGIMNIDQLRMFLEEIQGEESDIK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           AQ IF NLKH N FQ RGL LEDFF+YLLGDLNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLNIFQRRGLHLEDFFRYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+KN I VLHGG  TLTA V LIKC
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGG--TLTAPVALIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVTVTFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTVTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKPQ-DVDEDHL-EEDKDED 300
           LK +ILISTKPPEHNK ESTK+KP ADKQRDT+DDDIW S +PQ D+DEDHL EEDKDED
Sbjct: 241 LKGRILISTKPPEHNKVESTKEKPPADKQRDTADDDIWESVRPQEDMDEDHLVEEDKDED 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGS+LQTFFND EKVSRLSLSE+EL+ A  N+G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSDLQTFFNDIEKVSRLSLSEQELENAVTNYGHDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKG R DSSNYNPML WTHGAQMVAFNMQGYG+YLWIMEGMF+GN GCGYIKKP
Sbjct: 361 NLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFKGNDGCGYIKKP 420

Query: 421 DFLLNNP-----NSKSTPSTTENCLKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLL NP     NS+ST ST    LKVKVYMG GWHLEFGLSHFDFYSPPD YVKI IVG
Sbjct: 421 DFLLTNPDHKTSNSRSTSSTMIKRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKIRIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   ++T  ++DQW+PVW+EEFSFSI  PELALL++VVRD+D SG D FAGQT LPV
Sbjct: 481 VREDTVTRRTIPVEDQWVPVWNEEFSFSISTPELALLQLVVRDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           +ELRSGIRAVPLYNRKGE+YKHVKLLMRF  +
Sbjct: 541 KELRSGIRAVPLYNRKGERYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. NCBI nr
Match: XP_023519261.1 (phosphoinositide phospholipase C 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.3 bits (2276), Expect = 4.4e-252
Identity = 442/572 (77.27%), Postives = 486/572 (84.97%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY H+FRVCFCF RRF T   + PEDVK +FD+YSENGTMNI+QL+ FLEE+QGE+S ++
Sbjct: 1   MYRHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESKIK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A  IF NLKH N FQ RGL LEDFF+YLLGDLNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFRYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+ N I VLHGG  TLTA VKLIKC
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKNNGINVLHGG--TLTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVT TFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHLEE-DKDED 300
           LK +ILISTKPPEH K ESTK+KPSADKQRDT+DDDIW SA   +D +ED+LEE DKDE+
Sbjct: 241 LKGRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTNEDYLEEDDKDEE 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGSNLQTFFND EKVSRLSLSE+EL+ AT N G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGHDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKGSR DSSNYNPMLAWTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYIKKP
Sbjct: 361 NLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKKP 420

Query: 421 DFLLNNPNSKSTPSTTENC-----LKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLLNN   K++ S + +      LK+KVYMG GWHLEFGLSHFDFYSPPD YVKI IVG
Sbjct: 421 DFLLNNSEQKNSTSRSTSLIMIKRLKLKVYMGEGWHLEFGLSHFDFYSPPDLYVKIRIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   K+T  I+DQW+PVW+EEFSFSI APELALL+VVV D+D SG D FAGQT LPV
Sbjct: 481 VREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           RELRSGIRAVPLYNRKGE YKHVKLLMRF  +
Sbjct: 541 RELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. NCBI nr
Match: KAG6583361.1 (Phosphoinositide phospholipase C 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880.2 bits (2273), Expect = 9.8e-252
Identity = 441/572 (77.10%), Postives = 487/572 (85.14%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+H+FRVCFCF RRF T   + PEDVK +FD+YSENGTMNI+QL+ FLEE+QGE+S+M+
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLRMFLEEVQGEESEMK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A  IF NLKH N FQ RGL LEDFF+YLLGDLNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFRYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+KN I VLHGG  T TA VKLIKC
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGG--TFTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVT TFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHLEE-DKDED 300
           LK +ILISTKPPEH K ESTK+KPSADKQRDT+DDDIW SA   +D DED+LEE DKDE+
Sbjct: 241 LKGRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTDEDYLEEDDKDEE 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGSNLQTFFN+ EKVSRLSLSE+EL+ AT N G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSNLQTFFNEIEKVSRLSLSEQELENATGNCGRDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKGSR DSSNYNPMLAWTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYIKKP
Sbjct: 361 NLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKKP 420

Query: 421 DFLLNNPNSKSTPSTTENC-----LKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLLNN   +++ S + +      LK+KVYMG GW LEFG SHFDFYSPPD YVKIGIVG
Sbjct: 421 DFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGHSHFDFYSPPDLYVKIGIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   K+T  I+DQW+PVW+EEFSFSI APELALL+VVV D+D SG D FAGQT LPV
Sbjct: 481 VREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           RELRSGIRAVPLYNRKGE YKHVKLLMRF  +
Sbjct: 541 RELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. NCBI nr
Match: XP_022964953.1 (phosphoinositide phospholipase C 2-like [Cucurbita moschata])

HSP 1 Score: 879.8 bits (2272), Expect = 1.3e-251
Identity = 442/572 (77.27%), Postives = 486/572 (84.97%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+H+FRVCFCF RRF T   + PEDVK +FD+YSENGTMNI+QL+ FLEE+QGE+S+M+
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A  IF NLKH N FQ RGL LEDFFQYLLGDLNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFQYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+KN I VLHGG  T TA VKLIKC
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGG--TFTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVT TFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHLEE-DKDED 300
           LK +ILISTKPPEH K ESTK+KPSADKQRDT+DDDIW SA   +D DED+LEE DKDE+
Sbjct: 241 LKGRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTDEDYLEEDDKDEE 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGSNLQTFFND EKVSRLSLSE+EL+ AT N G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKGSR DSSNYNPMLAWTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYIKKP
Sbjct: 361 NLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKKP 420

Query: 421 DFLLNNPNSKSTPSTTENC-----LKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLLNN   +++ S + +      LK+KVYMG GW LEFG SHFDFYSPPD YVKI IVG
Sbjct: 421 DFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGHSHFDFYSPPDLYVKIRIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   K+T  I+DQW+PVW+EEFSFSI APELALL+VVV D+D SG D FAGQT LPV
Sbjct: 481 VREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           RELRSGIRAVPLYNRKGE YKHVKLLMRF  +
Sbjct: 541 RELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. NCBI nr
Match: XP_022970608.1 (phosphoinositide phospholipase C 2-like [Cucurbita maxima])

HSP 1 Score: 875.5 bits (2261), Expect = 2.4e-250
Identity = 439/572 (76.75%), Postives = 486/572 (84.97%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           MY+H+FRVCFCF RRF T   + PEDVK +FD+YSENGTMNI+QL+ FLEE+QGE+S+M+
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 120
           A  IF NLKH N FQ RGL LEDFF+YLLG+LNLA  P    YQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFRYLLGELNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 180
           SYLTGNQL S SSVTPII+AL+RGVR IELDLWP+S+KN I VLHGG  TLTA VKLIKC
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGINVLHGG--TLTAPVKLIKC 180

Query: 181 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 240
           LRAI+DHAF AS+YPVVITFEDHLTHDLR++VAKMVT TFGD+LYVPKSED NEFPSPES
Sbjct: 181 LRAIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPES 240

Query: 241 LKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSA-KPQDVDEDHLEE-DKDED 300
           LK +ILISTKPPEH K ESTK+KPSADK+RDT+DDDIW S    +D DED+LEE DKDE+
Sbjct: 241 LKGRILISTKPPEHTKVESTKEKPSADKRRDTADDDIWESTMSREDTDEDYLEEDDKDEE 300

Query: 301 IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEEELKIATKNHGDEIISFTQR 360
           I +PEYRSLIAIH  KMKRGSNLQTFFND EKVSRLSLSE+EL+ AT N G +II FTQR
Sbjct: 301 IVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQR 360

Query: 361 NLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCGYIKKP 420
           NLLRVYPKGSR DSSNYNPMLAWTHGAQMVAFNMQGYG+YLWIMEGMFRGNGGCGYIKKP
Sbjct: 361 NLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKKP 420

Query: 421 DFLLNNPNSKSTPSTTENC-----LKVKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVG 480
           DFLLNN   +++ S + +      LK+KVYMG GW LEFG SHFDFYSPPD YVKI IVG
Sbjct: 421 DFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGPSHFDFYSPPDLYVKIRIVG 480

Query: 481 DPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPV 540
             ED   K+T  I+DQW+PVW+EEFSFSI APELALL+VVV D+D SG D FAGQT LPV
Sbjct: 481 VREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLPV 540

Query: 541 RELRSGIRAVPLYNRKGEKYKHVKLLMRFNLD 566
           RELRSGIRAVPLYNRKGE YKHVKLLMRF  +
Sbjct: 541 RELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of Lcy10g000790 vs. TAIR 10
Match: AT3G08510.1 (phospholipase C 2 )

HSP 1 Score: 588.2 bits (1515), Expect = 7.1e-168
Identity = 312/587 (53.15%), Postives = 393/587 (66.95%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           M +  ++VCFCF RRF     + P ++K +F++YSENG M ++ L RFL ++Q +    R
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLN--LALLPPHQAYQDMGAPLSHYYIF 120
             AQ+I  +    +     GL L+ FF+YL GD N  LAL   H+ + DM AP+SHY+IF
Sbjct: 61  EDAQSIINSAS--SLLHRNGLHLDAFFKYLFGDNNPPLAL---HKVHHDMDAPISHYFIF 120

Query: 121 TGHNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVK 180
           TGHNSYLTGNQL S  S  PII AL++GVRVIELD+WPNS K+DI VLHG   TLT  V 
Sbjct: 121 TGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHG--MTLTTPVG 180

Query: 181 LIKCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPK-SEDSNEF 240
           LIKCL+AIR HAF+ SDYPVV+T EDHLT DL+ KVA+MVT  FG++L+ P   E   EF
Sbjct: 181 LIKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEF 240

Query: 241 PSPESLKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKP------------ 300
           PSP SLKR+I+ISTKPP+  K    K      K +D  D+++W    P            
Sbjct: 241 PSPNSLKRRIIISTKPPKEYK--EGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKD 300

Query: 301 ----QDVDEDHLEEDKDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEE 360
                D D+D  +EDK +  A P+Y+ LIAIH GK K G   +    D +KV RLSLSEE
Sbjct: 301 DLDGNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPK-GGITECLKVDPDKVRRLSLSEE 360

Query: 361 ELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYL 420
           +L+ A + +  +I+ FTQ NLLR+YPKG+R  SSNYNP++ W+HGAQMVAFNMQGYGR L
Sbjct: 361 QLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSL 420

Query: 421 WIMEGMFRGNGGCGYIKKPDFLLNNPNSKS--TPSTT---ENCLKVKVYMGVGWHLEFGL 480
           W+M+GMFR NGGCGYIKKPD LL + +      P  T   +  L+V VYMG GW+ +F  
Sbjct: 421 WLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRH 480

Query: 481 SHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVV 540
           +HFD YSPPDFY ++GI G P D   KKT T++D WIP WDE F F +  PELALL++ V
Sbjct: 481 THFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEV 540

Query: 541 RDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMR 562
            +YD S  D F GQT LPV EL  GIRA PL++RKGEKYK VKLL++
Sbjct: 541 HEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVK 577

BLAST of Lcy10g000790 vs. TAIR 10
Match: AT3G08510.2 (phospholipase C 2 )

HSP 1 Score: 588.2 bits (1515), Expect = 7.1e-168
Identity = 312/587 (53.15%), Postives = 393/587 (66.95%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           M +  ++VCFCF RRF     + P ++K +F++YSENG M ++ L RFL ++Q +    R
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLN--LALLPPHQAYQDMGAPLSHYYIF 120
             AQ+I  +    +     GL L+ FF+YL GD N  LAL   H+ + DM AP+SHY+IF
Sbjct: 61  EDAQSIINSAS--SLLHRNGLHLDAFFKYLFGDNNPPLAL---HKVHHDMDAPISHYFIF 120

Query: 121 TGHNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVK 180
           TGHNSYLTGNQL S  S  PII AL++GVRVIELD+WPNS K+DI VLHG   TLT  V 
Sbjct: 121 TGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHG--MTLTTPVG 180

Query: 181 LIKCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPK-SEDSNEF 240
           LIKCL+AIR HAF+ SDYPVV+T EDHLT DL+ KVA+MVT  FG++L+ P   E   EF
Sbjct: 181 LIKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEF 240

Query: 241 PSPESLKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKP------------ 300
           PSP SLKR+I+ISTKPP+  K    K      K +D  D+++W    P            
Sbjct: 241 PSPNSLKRRIIISTKPPKEYK--EGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKD 300

Query: 301 ----QDVDEDHLEEDKDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLSLSEE 360
                D D+D  +EDK +  A P+Y+ LIAIH GK K G   +    D +KV RLSLSEE
Sbjct: 301 DLDGNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPK-GGITECLKVDPDKVRRLSLSEE 360

Query: 361 ELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYL 420
           +L+ A + +  +I+ FTQ NLLR+YPKG+R  SSNYNP++ W+HGAQMVAFNMQGYGR L
Sbjct: 361 QLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSL 420

Query: 421 WIMEGMFRGNGGCGYIKKPDFLLNNPNSKS--TPSTT---ENCLKVKVYMGVGWHLEFGL 480
           W+M+GMFR NGGCGYIKKPD LL + +      P  T   +  L+V VYMG GW+ +F  
Sbjct: 421 WLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRH 480

Query: 481 SHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVV 540
           +HFD YSPPDFY ++GI G P D   KKT T++D WIP WDE F F +  PELALL++ V
Sbjct: 481 THFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEV 540

Query: 541 RDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMR 562
            +YD S  D F GQT LPV EL  GIRA PL++RKGEKYK VKLL++
Sbjct: 541 HEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVK 577

BLAST of Lcy10g000790 vs. TAIR 10
Match: AT5G58670.1 (phospholipase C1 )

HSP 1 Score: 578.6 bits (1490), Expect = 5.7e-165
Identity = 297/582 (51.03%), Postives = 395/582 (67.87%), Query Frame = 0

Query: 3   EHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGE-KSDMR- 62
           + +F+VCFC  R F  K  +PPE++KN+F +YS++  M+ +++ RF+ ++QGE  +D+  
Sbjct: 2   KESFKVCFCCVRNFKVKSSEPPEEIKNLFHDYSQDDRMSADEMLRFVIQVQGETHADINY 61

Query: 63  AQTIFKNLKHPNTFQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTGHN 122
            + IF  LKH   F  RG+ LE F++YLL D N  L    + +QDM  PLSHY+++TGHN
Sbjct: 62  VKDIFHRLKHHGVFHPRGIHLEGFYRYLLSDFNSPLPLTREVWQDMNQPLSHYFLYTGHN 121

Query: 123 SYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLIKC 182
           SYLTGNQL S SS+ PI+KAL  GVRVIELDLWPNS   + +V HGG  TLT+   L KC
Sbjct: 122 SYLTGNQLNSNSSIEPIVKALRNGVRVIELDLWPNSSGKEAEVRHGG--TLTSREDLQKC 181

Query: 183 LRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKSEDSNEFPSPES 242
           L  ++++AF  S YPVV+T EDHLT  L++KVAKMV+ TFG  L+    E +  FPSPES
Sbjct: 182 LNVVKENAFQVSAYPVVLTLEDHLTPILQKKVAKMVSKTFGGSLFQCTDETTECFPSPES 241

Query: 243 LKRKILISTKPPEH------NKGESTKKKPSADKQRDTSDDDIWVSAKPQDVDEDHLEED 302
           LK KILISTKPP+       +KG +T +   A K  D                E+ ++E+
Sbjct: 242 LKNKILISTKPPKEYLQTQISKGSTTDESTRAKKISDA---------------EEQVQEE 301

Query: 303 KDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFN-DTEKVSRLSLSEEELKIATKNHGDEII 362
            +E +A+ EYR LI+IH G  K G  L+   N D  +V RLS+SE+ L+   K  G +++
Sbjct: 302 DEESVAI-EYRDLISIHAGNRKGG--LKNCLNGDPNRVIRLSMSEQWLETLAKTRGPDLV 361

Query: 363 SFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGYGRYLWIMEGMFRGNGGCG 422
            FTQRNLLR++PK +RFDSSNY+P++ W HGAQMVAFNMQ +GRYLW+M+GMF+ NGGCG
Sbjct: 362 KFTQRNLLRIFPKTTRFDSSNYDPLVGWIHGAQMVAFNMQSHGRYLWMMQGMFKANGGCG 421

Query: 423 YIKKPDFLLNN--------PNSKSTPSTTENCLKVKVYMGVGWHLEFGLSHFDFYSPPDF 482
           Y+KKPD LL+N        P S++ P  T   LKVK+Y G GW+++F L HFD YSPPDF
Sbjct: 422 YVKKPDVLLSNGPEGEIFDPCSQNLPIKT--TLKVKIYTGEGWNMDFPLDHFDRYSPPDF 481

Query: 483 YVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKVVVRDYDGSGTDKF 542
           Y K+GI G P D A  +T   KD+W P+WD+EF F +R PEL+LL + V+DYD +  + F
Sbjct: 482 YAKVGIAGVPLDTASYRTEIDKDEWFPIWDKEFEFPLRVPELSLLCITVKDYDSNTQNDF 541

Query: 543 AGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMRFNLDER 568
           AGQT  P+ E+R GIRAV L++R GE YKHV+LLMRF L+ R
Sbjct: 542 AGQTCFPLSEVRPGIRAVRLHDRAGEVYKHVRLLMRFVLEPR 561

BLAST of Lcy10g000790 vs. TAIR 10
Match: AT3G55940.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 559.7 bits (1441), Expect = 2.7e-159
Identity = 304/589 (51.61%), Postives = 390/589 (66.21%), Query Frame = 0

Query: 1   MYEHNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYSENGTMNIEQLKRFLEEIQGEKSDMR 60
           M +  ++VCFCF RR+       P ++K +FD YS+ G M  + L RFL ++Q  K D  
Sbjct: 1   MSKQTYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGLMTTDLLLRFLIDVQ--KQDKA 60

Query: 61  AQTIFKNLKHPNT--FQNRGLCLEDFFQYLLGDLNLALLPPHQAYQDMGAPLSHYYIFTG 120
            +   +++ + ++      GL L+ FF+YL    N + L   + +QDM APLSHY+I+TG
Sbjct: 61  TKEEAQDIVNASSSLLHRNGLHLDAFFKYLFAVTN-SPLSSLEVHQDMDAPLSHYFIYTG 120

Query: 121 HNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDIKVLHGGCRTLTASVKLI 180
           HNSYLTGNQL S  S  PII+AL++GVRVIELD+WPNS ++ I VLHG  RTLT+ V+LI
Sbjct: 121 HNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHG--RTLTSPVELI 180

Query: 181 KCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFGDMLYVPKS-EDSNEFPS 240
           KCLRAIR+HAF+ SDYPVV+T EDHLT  L+ KVA+MVT  FG+ML+ P S E   EFPS
Sbjct: 181 KCLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFPS 240

Query: 241 PESLKRKILISTKPPEHNKGESTKKKPSADKQRDTSDDDIWVSAKPQ------------- 300
           P  LK++I+ISTKPP+  K  +        K RD  D ++W    P              
Sbjct: 241 PAFLKKRIMISTKPPKEYK--AATDDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDKNDS 300

Query: 301 --DVDEDHLEEDKDED-------IAVPEYRSLIAIHGGKMKRGSNLQTFFNDTEKVSRLS 360
             D D+D  ++D D+D        A PEY+ LIAI  GK K G   +    D +KV RLS
Sbjct: 301 NGDDDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPK-GGITECLKVDPDKVRRLS 360

Query: 361 LSEEELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQMVAFNMQGY 420
           LSEE+L+ A++ +  +I+ FTQRNLLRVYPKG+R  SSNYNP++AW+HGAQMVAFNMQG 
Sbjct: 361 LSEEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNMQGL 420

Query: 421 GRYLWIMEGMFRGNGGCGYIKKPDFLLNNPNSKSTPSTT---ENCLKVKVYMGVGWHLEF 480
           GR LW+M+GMFRGNGGCGYIKKPD LL + N+   P  T   +  L+V +YMG GW+ +F
Sbjct: 421 GRSLWVMQGMFRGNGGCGYIKKPDLLLKS-NAVFDPEATLPVKTTLRVTIYMGEGWYYDF 480

Query: 481 GLSHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFSFSIRAPELALLKV 540
             +HFD YSPPDFY ++GI G P D   KKT T++D WIP WDE F F +  PELALL++
Sbjct: 481 PHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRI 540

Query: 541 VVRDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLLMR 562
            V +YD S  D F GQ  LPV ELR GIRAVPL N+ G K + VKLL+R
Sbjct: 541 EVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVR 580

BLAST of Lcy10g000790 vs. TAIR 10
Match: AT2G40116.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.4e-155
Identity = 297/603 (49.25%), Postives = 384/603 (63.68%), Query Frame = 0

Query: 4   HNFRVCFCFWRRFWTKKPDPPEDVKNMFDEYS-----------------ENGTMNIEQLK 63
           +N+R+   + R+F   +  P +DV++ F +++                   G M  EQL 
Sbjct: 17  YNYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLC 76

Query: 64  RFLEEIQGEKSDMRAQ----TIFKNLKHPNTFQNRGLCLEDFFQYLL-GDLNLALLPPHQ 123
            FL++     +   AQ     + +   H   F   GL L+DFF +L   DLN  + P   
Sbjct: 77  SFLDDHGESTTVAEAQRLIDEVIRRRHHVTRFTRHGLDLDDFFNFLFYDDLNPPITP--H 136

Query: 124 AYQDMGAPLSHYYIFTGHNSYLTGNQLWSRSSVTPIIKALERGVRVIELDLWPNSRKNDI 183
            +QDM APLSHY+I+TGHNSYLTGNQL S  S  P+IKAL+RGVRVIELDLWPNS   DI
Sbjct: 137 VHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDI 196

Query: 184 KVLHGGCRTLTASVKLIKCLRAIRDHAFNASDYPVVITFEDHLTHDLREKVAKMVTVTFG 243
            VLHG  RTLT  V L+KCL++IRD+AF++S YPV+IT EDHLT DL+ KVA+M T  FG
Sbjct: 197 NVLHG--RTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFG 256

Query: 244 DMLYVPKSEDSNEFPSPESLKRKILISTKPP--------------EHNKGESTKKKPSAD 303
            MLY P+S+   EFPSP SL  +I+ISTKPP              ++N   S++ +    
Sbjct: 257 QMLYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRT 316

Query: 304 KQRDTSDDDIWVSAKPQDVDEDHLEEDKDEDIAVPEYRSLIAIHGGKMKRGSNLQTFFND 363
           ++  T +  ++        D D  +E+  ED   P Y+ LI IH GK K G+  +     
Sbjct: 317 EEIQTLESMLFDQDFESKSDSDQEDEEASED-QKPAYKRLITIHAGKPK-GTVKEEMKVV 376

Query: 364 TEKVSRLSLSEEELKIATKNHGDEIISFTQRNLLRVYPKGSRFDSSNYNPMLAWTHGAQM 423
            +KV RLSLSE+EL     ++  +++ FTQRNLLR+YPKG+RF+SSNY P++ WTHGAQM
Sbjct: 377 VDKVRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQM 436

Query: 424 VAFNMQGYGRYLWIMEGMFRGNGGCGYIKKPDFLLN--------NPNSKSTPSTTENCLK 483
           +AFNMQGYG+ LW+M GMFR NGGCGY+KKP+FL+         +P  K     T   LK
Sbjct: 437 IAFNMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKET---LK 496

Query: 484 VKVYMGVGWHLEFGLSHFDFYSPPDFYVKIGIVGDPEDKARKKTTTIKDQWIPVWDEEFS 543
           VKVYMG GW ++F  +HFD YSPPDFY K+ IVG P D A+KKT  I+D W P+WDEEFS
Sbjct: 497 VKVYMGDGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFS 556

Query: 544 FSIRAPELALLKVVVRDYDGSGTDKFAGQTFLPVRELRSGIRAVPLYNRKGEKYKHVKLL 563
           F +  PELALL++ VR+YD S  D F GQT LPV ELR GIR+VPLY++KGEK K V+LL
Sbjct: 557 FPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKMKSVRLL 610