Lcy10g000560 (gene) Sponge gourd (P93075) v1

BLAST of Lcy10g000560 vs. ExPASy Swiss-Prot
Match: Q6NUV0 (Rab3 GTPase-activating protein catalytic subunit OS=Danio rerio OX=7955 GN=rab3gap1 PE=2 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 9.9e-50
Identity = 220/879 (25.03%), Postives = 366/879 (41.64%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDATQLEISTNLYKVKS-DL 77
           + EV    DFT AS WERFIS +E V   W   G  + + K   + E ++  ++  S ++
Sbjct: 8   ESEVFEITDFTTASEWERFISRVEEVLNDWKLIG--SRVGKPLEKGEYTSGTWEESSQEI 67

Query: 78  KCDNKNYSMEYYF----GNNNNGKVV------------------DWELRLHELQLCFGVQ 137
              +  +S+ +++       N+G+                    D+  R H L   FGV+
Sbjct: 68  SFADFKFSITHHYLKQESAENDGRDELEEDAYPLAMQDLLCINNDFPPRAHCLVRWFGVR 127

Query: 138 EFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGT 197
           EF+VI+P +    I+   + S LLS+V+IAL N     P FV +    RK + G +  G 
Sbjct: 128 EFVVISPGANCEAIISESKCSLLLSSVSIALANTGCQVPLFVQIQQKHRKMFSG-ECQGP 187

Query: 198 TFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLS 257
                FE   +  +VP +  HL GL ++F +K       +      + FT  L       
Sbjct: 188 GVRSDFEMVHL-RRVPSQYNHLSGLLDIFKNKIGCPLTPLPPINISIRFTYVL------- 247

Query: 258 DDDDIRRADAEMTESAHESAL-GIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMV 317
              D ++          ++ L G  G V++    P+    + EDP+    L A+W     
Sbjct: 248 --QDWQQCSWPQQPPDFDALLAGEVGGVEF-GKLPFG---ACEDPISELHLAATWPSLTE 307

Query: 318 ESSLEMAELENSSPHEAKKWILLPILSPN--------------------ITDSSMGNRVG 377
              ++     +  P +A  W +    + N                     T+  +G    
Sbjct: 308 GIVVDNDVYSDLDPLQAPHWSVRVRTAENPQCLLGEFLTEFFKICCRKETTEEILGRSTA 367

Query: 378 FS------------SQL----------YLLIDALDMSFQAQFMEDFVSIENPGSDNLKSS 437
                         S+L           L + ++  S + +        E+P ++++ +S
Sbjct: 368 EEEGKENSDITQALSKLTEPSTAVPIHKLSVSSMVHSARKRIRRHRRVPESPLNNDVLNS 427

Query: 438 TVVP--PPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQ--FCLHVLWF 497
            ++   P   +D+         ++SP     +      +K+   DSL  +   C+ ++ F
Sbjct: 428 ILLYLFPDAALDKADVSEVRPPQQSPDRQSEDYHLYNQLKSCPSDSLTHRLALCICMVNF 487

Query: 498 GNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLAICIEKKH 557
            +  +RA+A LW EFV E+R+ WE    L     S   DL  CL++QKLQML  CIE+K 
Sbjct: 488 HHGGVRAVAHLWQEFVLEMRYRWEN-NCLIYGLASGPPDLRCCLLHQKLQMLNCCIERKK 547

Query: 558 QSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVEFESKANL 617
              +  +        D  R   +G   E     +  G+++D   DS    E EF    + 
Sbjct: 548 ARDDGKKSSSSDGARDRSRGAPEGAGPEGAGPAEAAGKSWDSWSDS----EDEFFECVSD 607

Query: 618 FKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLMTEDMHEE 677
            ++  + ++      +  P+    G +   GT  LLN+ + ++ P TQ+   MTED+ EE
Sbjct: 608 TEEMKEDKEEAENRSRSKPE----GRLQPHGTHTLLNTQEPLYIPITQEPAPMTEDLLEE 667

Query: 678 RLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEEDSDP 737
           + + +   G S +     A+++   L SDM +FKAANP     DF+RW+SP D+ E+   
Sbjct: 668 QSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCTLLDFVRWYSPRDYVEEQVT 727

Query: 738 KDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREG 797
             +G                RG LS RM   GN+W + W  A A P   QK L D  +E 
Sbjct: 728 DADG------------RVEVRGELSARMKIPGNMWVEAWETARATPARRQKRLFDDTKEA 787

Query: 798 EKILHYLETLRPHQLLEQMV-CTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSVLKF 823
           EK+LHYL   +P +L   ++ C    A      + S   L  +++ ++Q+  +   +L+ 
Sbjct: 788 EKVLHYLALQKPSELTRHLLPCVLHAALLKIKEEESAEDLPSVRSSLQQISSSASKILRH 840

BLAST of Lcy10g000560 vs. ExPASy Swiss-Prot
Match: Q642R9 (Rab3 GTPase-activating protein catalytic subunit OS=Xenopus laevis OX=8355 GN=rab3gap1 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 4.2e-48
Identity = 228/899 (25.36%), Postives = 381/899 (42.38%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDATQLEISTNLYKVKSD-- 77
           + EV    DFT AS WERFIS++E V  +W   G  +   K   + E ++ +++ K +  
Sbjct: 8   ESEVFEITDFTTASEWERFISKVEEVLTEWKLIGETS--NKPPEKGEYTSGVWEEKGEEV 67

Query: 78  LKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLC---------------------FGVQ 137
           L  D +     +Y    ++ K    +     + +C                     +G++
Sbjct: 68  LFADFRFSIRHHYLVQKSSEKEEKEDTGEDSIPVCMQDLLCTNNDFPPVAHCLVRWYGLR 127

Query: 138 EFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGT 197
           EF+VI+P +   VI +S + + LLS+V+IAL N     P FV VH   RK Y+G +  G 
Sbjct: 128 EFVVISPGANDAVISES-KCNLLLSSVSIALGNTGCQVPLFVQVHQKWRKLYVG-ECRGP 187

Query: 198 TFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLS 257
                FE   +  +VP +  HL GL ++F SK         T L  V+  ++ T+  +L 
Sbjct: 188 GVRTDFEMVHL-RKVPNQYTHLSGLLDIFKSKIGCP----LTALPPVNIAIRFTY--VLQ 247

Query: 258 DDDDI----RRADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASW-- 317
           D        +  D +         L   GK+ +          + EDP+    L  +W  
Sbjct: 248 DWQQYFWPQQPPDIDALIGGEVGGLEF-GKLPFG---------ACEDPISELHLATTWPC 307

Query: 318 -SEKMVESSLEMAELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDM 377
            +E ++  +   ++L+   P +A +W +    + N     +G+   F S+ + L    + 
Sbjct: 308 LTEGIIVDNDVYSDLD---PLQAPQWSVRVRKADN-PQCMLGD---FVSEFFRLCRRKES 367

Query: 378 SFQAQFMEDFVSIENPGSD--NLKSSTVVPPPTVIDRV-LKELFHEGKKSPYFAKGEDR- 437
           + +      F      G+D     S    P P  I ++ +  + H  +K     +G D  
Sbjct: 368 TDELLGKSAFEENGKEGADISQALSKLTEPAPVPIHKLSVTSMVHSARKKIRKHRGADES 427

Query: 438 -----------------------------------TSQA----------IKAADLDSLFA 497
                                              +SQ+          +K+A  +SL  
Sbjct: 428 PLNNDVLNAILFFLFPDTKSLDGSEAKPSTSTGNISSQSESEDYNLYSQLKSAPSNSLTY 487

Query: 498 Q--FCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKL 557
           +   CL ++ F +  ++ +A LW EFV E+R+ WE    +P +  + S DL  CL++QKL
Sbjct: 488 KLALCLCMVNFYHGGVKGVAHLWQEFVLEMRYRWENNFLIPGL-ANGSPDLKCCLLHQKL 547

Query: 558 QMLAICIEKKHQSIE--EFQDCLGSQDPDSPRAEED--GPVSEDFSVMQTPGENFDGKCD 617
           QML  C+E+K    E  +      S +    +   D   PV  D S  +   +++D   D
Sbjct: 548 QMLNCCLERKKARDEGKKGNPLYSSSESSVNKTASDLLSPVEADKSKYEV-AKSWDSWSD 607

Query: 618 SHLSPEVEFESKAN---LFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSM 677
           S    E  FE  ++   L +   +S        +++P     G +   G +MLLNS + +
Sbjct: 608 SE---EEFFECHSDTEELKESGQESARKAKEETKENPSPKPEGRLHQSGNLMLLNSGEPL 667

Query: 678 HAPFTQDVPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVF 737
           + P TQD   MT+D+ EE+ + +   G S +     A+++   L SDM +FKAANP    
Sbjct: 668 YIPVTQDPAPMTDDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCCL 727

Query: 738 EDFIRWHSPGDWEEDSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDA 797
           EDF+RW+SP D+ E+    D+G            N   +G LS RM    N+W + W  A
Sbjct: 728 EDFVRWYSPRDYIEEEVMDDKG------------NKIFKGELSARMKIPNNMWVEAWETA 787

Query: 798 PALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMV-CTAFKAAADTLSQTSYGGLKL 825
             +P   Q+ L D  +E EK+LHYL   +P  L   ++ C    A      + +   +  
Sbjct: 788 KPIPARRQRRLFDDTKEAEKVLHYLAVQKPADLTRHLLPCVIHAALLKLKEEEAAEDIPS 847

BLAST of Lcy10g000560 vs. ExPASy Swiss-Prot
Match: Q15042 (Rab3 GTPase-activating protein catalytic subunit OS=Homo sapiens OX=9606 GN=RAB3GAP1 PE=1 SV=3)

HSP 1 Score: 193.7 bits (491), Expect = 9.3e-48
Identity = 235/894 (26.29%), Postives = 364/894 (40.72%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK-----------DATQLEIS 77
           + EV    DFT AS WERFIS++E V   W   G N+L K            +    EIS
Sbjct: 8   ESEVFEITDFTTASEWERFISKVEEVLNDWKLIG-NSLGKPLEKGIFTSGTWEEKSDEIS 67

Query: 78  --------TNLYKV--------KSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLC 137
                   T+ Y V        K +L  D    SM+   G NN     D+  R H L   
Sbjct: 68  FADFKFSVTHHYLVQESTDKEGKDELLEDVVPQSMQDLLGMNN-----DFPPRAHCLVRW 127

Query: 138 FGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQ 197
           +G++EF+VIAP + S  +L   + + LLS+V+IAL N     P FV +H   R+ Y+G +
Sbjct: 128 YGLREFVVIAPAAHSDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQIHHKWRRMYVG-E 187

Query: 198 NMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFR 257
             G      FE   +  +VP +  HL GL ++F SK       +      + FT      
Sbjct: 188 CQGPGVRTDFEMVHL-RKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVSIAIRFT------ 247

Query: 258 ILLSDDDDI----RRADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIA 317
            +L D        +  D +         L   GK+ +          + EDP+    L  
Sbjct: 248 YVLQDWQQYFWPQQPPDIDALVGGEVGGLEF-GKLPFG---------ACEDPISELHLAT 307

Query: 318 SWSEKMVESSLEMAELENSSPHEAKKWILLPILSPN--------------------ITDS 377
           +W        ++     +  P +A  W +    + N                     TD 
Sbjct: 308 TWPHLTEGIIVDNDVYSDLDPIQAPHWSVRVRKAENPQCLLGDFVTEFFKICRRKESTDE 367

Query: 378 SMGNRVGFSSQ----------LYLLIDALDMSFQAQFMEDFV-----------SIENPGS 437
            +G R  F  +          L  L +   +      + + V            +E    
Sbjct: 368 ILG-RSAFEEEGKETADITHALSKLTEPASVPIHKLSVSNMVHTAKKKIRKHRGVEESPL 427

Query: 438 DNLKSSTVVP---PPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQ--F 497
           +N   +T++    P  V ++ L         +P     +       K+A  DSL  +   
Sbjct: 428 NNDVLNTILLFLFPDAVSEKPLDGTTSTDNNNPPSESEDYNLYNQFKSAPSDSLTYKLAL 487

Query: 498 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 557
           CL ++ F +  ++ +A LW EFV E+R+ WE    +P +  S   DL  CL++QKLQML 
Sbjct: 488 CLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGL-ASGPPDLRCCLLHQKLQMLN 547

Query: 558 ICIEKKHQSIE----EFQDCLGSQDPDSPRAEED---GPVSEDFSVMQTPGENFDGKCDS 617
            CIE+K    E       D       D+ +A +      + E        G+++D   DS
Sbjct: 548 CCIERKKARDEGKKTSASDVTNIYPGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDS 607

Query: 618 HLSPEVEFESKANLFKDSTKSEDVISFTDQKSPDSMR-RGSVGIVGTMMLLNSYQSMHAP 677
               E  FE  ++  +     ++       K   ++R  G +   G + LL++ + ++ P
Sbjct: 608 E---EEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIP 667

Query: 678 FTQDVPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFEDF 737
            TQ+   MTED+ EE+ + +   G S +     A+++   L SDM +FKAANP    EDF
Sbjct: 668 VTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDF 727

Query: 738 IRWHSPGDWEEDSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPAL 797
           +RW+SP D+ E+ +  DE           K N   +G LS RM    N+W + W  A  +
Sbjct: 728 VRWYSPRDYIEE-EVIDE-----------KGNVVLKGELSARMKIPSNMWVEAWETAKPI 787

Query: 798 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMV-CTAFKAAADTLSQTSYGGLKLMKA 823
           P   Q+ L D  RE EK+LHYL   +P  L   ++ C    A      + S   +  +K 
Sbjct: 788 PARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKK 847

BLAST of Lcy10g000560 vs. ExPASy Swiss-Prot
Match: Q80UJ7 (Rab3 GTPase-activating protein catalytic subunit OS=Mus musculus OX=10090 GN=Rab3gap1 PE=1 SV=4)

HSP 1 Score: 192.6 bits (488), Expect = 2.1e-47
Identity = 230/890 (25.84%), Postives = 362/890 (40.67%), Query Frame = 0

Query: 18  DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPN--NLLKK--------DATQLEIS- 77
           + EV    DFT AS WERFIS++E V   W   GP+    L+K        +    EIS 
Sbjct: 8   ESEVFEITDFTTASEWERFISKVEEVLNDWKLIGPSLGKPLEKGIFTSGTWEERSDEISF 67

Query: 78  -------TNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVV---DWELRLHELQLCFGVQEF 137
                  T+ Y V+     + K+  +E     +    +    D+  R H L   +G++EF
Sbjct: 68  ADFRFSVTHHYLVQESPDKERKDEELEDAIPQSMQDLLCMNNDFPPRAHCLVRWYGLREF 127

Query: 138 LVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTF 197
           +VIAP + S  +L   + + LLS+++IAL N     P FV +H   R+ Y+G +  G   
Sbjct: 128 VVIAPAAHSDAVLSESKCNLLLSSISIALGNTGCQVPLFVQIHHKWRRMYMG-ECQGPGV 187

Query: 198 TRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDD 257
              FE   +  +VP +  HL GL ++F SK         T L  V   ++LT+  +L D 
Sbjct: 188 RTDFEMVHL-RKVPSQYTHLSGLLDIFKSKIGCP----LTPLPPVSIAIRLTY--VLQDW 247

Query: 258 DDI----RRADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKM 317
                  +  D +         L   GK+ +          + EDP+    L  +W    
Sbjct: 248 QQYFWPQQPPDIDALVGGEVGGLEF-GKLPFG---------ACEDPISELHLATTWPHLT 307

Query: 318 VESSLEMAELENSSPHEAKKWILLPILSPN--------------------ITDSSMGNR- 377
               ++     +  P +A  W +    + N                     TD  +G   
Sbjct: 308 EGIIVDNDVYSDLDPVQAPHWSVRVRKADNPQCLLGDFVTEFLKICRRKESTDEILGRST 367

Query: 378 --------VGFSSQLYLLIDALDMSFQAQFMEDFVSI------ENPGSDNLKSSTVVP-- 437
                      +  L  L +   +      + + V        ++ G ++  +S V+   
Sbjct: 368 FEEEGREVADITHALSKLTEPAPVPIHKLSVSNMVHTAKKKIRKHRGEESPLNSDVLNTI 427

Query: 438 -----PPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQ--FCLHVLWFG 497
                P  V ++ L          P    G+       K+A  DSL  +   CL ++ F 
Sbjct: 428 LLFLFPDAVSEKPLDGTTSIDNSIPAPEAGDYTLYNQFKSAPSDSLTYKLALCLCMINFY 487

Query: 498 NCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLAICIEKKHQ 557
           +  ++ +A LW EFV E+R+ WE    +P +  S S DL  CL++QKLQML  CIE+K  
Sbjct: 488 HGGLKGVAHLWQEFVLEMRFRWENNFLIPGL-ASGSPDLRCCLLHQKLQMLNCCIERKKA 547

Query: 558 SIEEFQDCLGSQDPDS---PRAEEDGPVSEDFSVMQTPGENFDGKC-DSHLSPEVEFESK 617
             E  +  L      +      +  G +  D        +   GK  DS    E EF   
Sbjct: 548 RDEGKKTSLSDSTTSAYPGDAGKTGGQLGLDHLRDTEKEKGEVGKSWDSWSDSEEEF--- 607

Query: 618 ANLFKDSTKSEDVISFTDQK----SPDSMR----RGSVGIVGTMMLLNSYQSMHAPFTQD 677
              F+  + +ED+     +      P  M      G +   G + LL++ + ++ P TQ+
Sbjct: 608 ---FECLSDTEDLKGNGQESGKKGGPKEMANLKPEGRLHQHGKLTLLHNGEPLYIPVTQE 667

Query: 678 VPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFEDFIRWH 737
              MTED+ EE+ + +   G S +     A+++   L SDM +FKAANP    EDF+RW+
Sbjct: 668 PAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWY 727

Query: 738 SPGDWEEDSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSE 797
           SP D+ E+ +  DE           K N   +G LS RM    N+W + W  A  +P   
Sbjct: 728 SPRDYIEE-EVTDE-----------KGNVVLKGELSARMKIPSNMWVEAWETAKPVPARR 787

Query: 798 QKSLLDPNREGEKILHYLETLRPHQLLEQMV-CTAFKAAADTLSQTSYGGLKLMKAKMEQ 823
           Q+ L D  RE EK+LHYL   +P  L   ++ C    A      + S   +  +K  ++Q
Sbjct: 788 QRRLFDDTREAEKVLHYLAMQKPADLARHLLPCVIHAAVLKVKEEESLENIPSVKKIIKQ 847

BLAST of Lcy10g000560 vs. ExPASy Swiss-Prot
Match: Q9VQ26 (Rab3 GTPase-activating protein catalytic subunit OS=Drosophila melanogaster OX=7227 GN=CG31935 PE=1 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 5.8e-34
Identity = 211/870 (24.25%), Postives = 347/870 (39.89%), Query Frame = 0

Query: 14  EALEDEEVEHFDDFTIASTWERFISEIEAVCRQW--MADGPNNLLKKDATQLEISTNLYK 73
           E ++D E  + ++F+  S WE F +++  + ++W   +D     LK +    EI +  +K
Sbjct: 3   EEIDDNEF-YRENFSADSDWEVFNAQLGEILQKWDVSSDSETRNLKSE----EIFSCNWK 62

Query: 74  VKSD-LKCDNKNYSMEYYFG------------------------------NNNNGKVVDW 133
           V+ + L        +EY+                                 N+ G  +  
Sbjct: 63  VEREKLDMLRNGIEVEYHQAILEDEELVRAEAKEITCLQRTSCHHDLMSTGNSFGPPIRS 122

Query: 134 ELRLHELQLCFGVQEFLVIAPQSIS-GVILDSPEASKLLSAVAIALTNCSSFWPAFVPVH 193
              LH L   +G++ F+V+ P + +   +  + E +  LSAVA+      S  P FV ++
Sbjct: 123 SQELHILARIYGLRRFIVLHPVNPTLNYMRSTSEFNFFLSAVAVVSAEVQSLVPIFVQIY 182

Query: 194 DPSRKAYIGIQNMGTTFTRRFEADRIGTQ-VPIKLMHLEGLYELFVSKFAYS-----SVD 253
           DP    Y G+  +       F    IG +  P +   L GL  LF  K   S      + 
Sbjct: 183 DPKWNYYTGVA-LAPALRTNFRL--IGLEKAPPECRFLMGLLTLFREKVPTSYTQAAMIS 242

Query: 254 IST----NLFKVHFTMKLTFRILLSDDDDIRRADAEMTESAHESALGIRGKVQWDDDCPW 313
           + T    +  ++   M + F   LS +D +   D E++             ++    C  
Sbjct: 243 VCTTYALDTMRIRMPMYVPFDHGLSSEDIV--VDGEVSH------------LEVQQFCAL 302

Query: 314 SEWYSSEDPVKGFELIASWSEKMVESSLEMAELENSSPHEA---KKWILLPILS------ 373
              Y  E   + + L+ +W E     + +  +  +  P +A   K ++ +   S      
Sbjct: 303 PHGYKPESRTEIY-LVYTWPELSEHVAFDSEQRSDFVPAKAPLGKIYLSVEASSYLSCCL 362

Query: 374 ---------PNITDSSMG-NRVGFSSQLYLLIDALDMSFQAQFMEDFV-SIENPGSDNLK 433
                        +S +G N  G SS      + LD   + +  +    S E P    L 
Sbjct: 363 RDYQSVAEVTRSLESFVGRNFSGTSSGAEAASNPLDRITEHKLTKRRERSFELPSQAGLT 422

Query: 434 SSTVVP-PPTVIDRVLKELF----HEGKKSPYFAKG--EDRTSQAIKAADLDSLFAQF-C 493
                P   + +  +L  LF     E    PY  K   +      IK+A  DSL  +  C
Sbjct: 423 KRLPGPMTESELSELLAYLFPDMHPEMALFPYAKKNFTDKFDPMRIKSAVPDSLVCRLSC 482

Query: 494 LHVLWFGNC-NIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 553
           L      +  ++  +A +W  F R++R  W+    +P +  +   D  TCL++QKLQML 
Sbjct: 483 LLATCHAHLGSVEGMAQVWAAFTRQLRLLWDNSLMVPGIS-AGFPDTRTCLLHQKLQMLN 542

Query: 554 ICIEKKHQ----SIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLS 613
           +C+E++ Q    S  + +  +G    +    E+D             GE FD  CD  L+
Sbjct: 543 VCVERRVQREANSKRKSEGMVGKASSEEEEDEDD-----------DEGEFFD--CDD-LT 602

Query: 614 PEVEFESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQD 673
                 +KA L                K    +RR    +    +L    + ++ P TQ+
Sbjct: 603 AGAGSPTKAVL--------------SLKPEGRLRR----LNNERLLEEPDEYLYIPDTQE 662

Query: 674 VPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPG 733
               TED  ++  + +   G     + Q+    L SDM AFKAANP G+ EDFIRW+SP 
Sbjct: 663 PVPKTEDQLQDDAEVMLKLGPGSGLTTQMMCTSLLSDMEAFKAANPRGIMEDFIRWYSPK 722

Query: 734 DWEEDSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKS 793
           DWEE +D   +                 +  LS RM+  GN W+++W  A A+PVS QK 
Sbjct: 723 DWEEVTDELGQ----------------VKHQLSIRMTTEGNTWQKVWEQAQAVPVSRQKR 782

Query: 794 LLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAA----ADTLSQTSYGGLKLMKAKMEQ 803
           L D   E  K+LHYLET + H++    V     +A    AD LS        L  +++E+
Sbjct: 783 LFDDTNEALKVLHYLETRKMHEIYNLTVIPLLHSAILKLADILSNAELE--DLFSSQIEK 797

BLAST of Lcy10g000560 vs. ExPASy TrEMBL
Match: A0A6J1K9B0 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111492852 PE=3 SV=1)

HSP 1 Score: 1788.1 bits (4630), Expect = 0.0e+00
Identity = 888/954 (93.08%), Postives = 915/954 (95.91%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASSSK ESNGEDEA+EDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA
Sbjct: 1   MASSSKAESNGEDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADRIGTQVPIKLMHLEGLYELFVSKFAYSS++IS NLFKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           R DAEMTES +ESA+G  GK QWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITD++MGNRVGFSSQLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKSPYF+KGE RTSQAIKAA LDSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCL SQDP+SPRAEEDGPVSED S MQTPGENFDGKCDSH SPEVE
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHSSPEVE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESKANLFK+ TKSEDVISFTDQKS DS RRGS GIVGTMMLLNSYQSMH+PFTQD PLM
Sbjct: 541 FESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHSPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           DSDPKDEGLPNG ATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCTAFKA ADTLSQTSYGGLKLMK KM+QLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSEAIFKDYY+F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTG S NKF TEF+KKQ VRSHERGAISS+FTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           ILREI+FSLRDRV+GNHYADSTP AR E +ETHRMYISGTSNDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Lcy10g000560 vs. ExPASy TrEMBL
Match: A0A6J1GBV8 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452560 PE=3 SV=1)

HSP 1 Score: 1786.9 bits (4627), Expect = 0.0e+00
Identity = 888/954 (93.08%), Postives = 914/954 (95.81%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASSSK ESNG DEA+EDEEVEHFDDFTIASTWERFISEIEAVCRQWM+DGPNNLLKKDA
Sbjct: 1   MASSSKAESNGGDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMSDGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DIS NLFKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           R DAEMTES +ESA+G  GK QWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITD++MGNRVGFSSQLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKSPYFAKGE RTSQAIKAA LDSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCL SQDP+SPRAEEDGPVSED S MQTPGENFDGKCDSHLSPEVE
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHLSPEVE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESK NLF++ TKSEDVISFTDQKS DS RRGS GIVGTMMLLNSYQSMHAPFTQD PLM
Sbjct: 541 FESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHAPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           DSDPKDEGLPNG ATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCTAFKA ADTLSQTSYGGLKLMK KM+QLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSEAIFKDYY+F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTG S NKF TEF+KKQ VRSHERGAISS+FTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           ILREI+FSLRDRV+GNHYADSTP AR E +ETHRMYISGTSNDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Lcy10g000560 vs. ExPASy TrEMBL
Match: A0A5A7UVM5 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00740 PE=3 SV=1)

HSP 1 Score: 1783.1 bits (4617), Expect = 0.0e+00
Identity = 880/954 (92.24%), Postives = 916/954 (96.02%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASS+KVESNGEDEAL+DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNY+MEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYTMEYYFGNSNHGKVVDWELNLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSSVDI TN FKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           RAD +M+ESA E+A+G  GKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RADTDMSESAQENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITDS+MGNRVGFS+QLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSTQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGE R SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCLGSQDP+SPR E+DG VSEDFS+MQTPGENFDGKCDSHLS E E
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGSVSEDFSIMQTPGENFDGKCDSHLSSEDE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
            ESKANL KDSTKSED+I+F DQKSPDSMRRGS GIVG MMLLNS+Q MHAPFTQDVPLM
Sbjct: 541 LESKANLLKDSTKSEDLITFADQKSPDSMRRGSAGIVGNMMLLNSHQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +S PKDE LP+G ATETSKNNW PRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDGSATETSKNNWRPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKAAADTLSQT+YGGLKLMK KMEQLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEV DDLRRLCVVFEHVEKLMALAASLHRK SQAPRLSE IFKDY+DF
Sbjct: 781 LKFLQGNRLSAESEVFDDLRRLCVVFEHVEKLMALAASLHRKFSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTGSSG+KF TEF+KKQ VRSHERG ISSMFTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           +LREIVFSLRDRVNGNHYADSTP AR EE++THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTANDLRVALSVTSCD 954

BLAST of Lcy10g000560 vs. ExPASy TrEMBL
Match: A0A1S3C6J8 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103497052 PE=3 SV=1)

HSP 1 Score: 1783.1 bits (4617), Expect = 0.0e+00
Identity = 880/954 (92.24%), Postives = 916/954 (96.02%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASS+KVESNGEDEAL+DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNY+MEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYTMEYYFGNSNHGKVVDWELNLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSSVDI TN FKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           RAD +M+ESA E+A+G  GKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RADTDMSESAQENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITDS+MGNRVGFS+QLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSTQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGE R SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCLGSQDP+SPR E+DG VSEDFS+MQTPGENFDGKCDSHLS E E
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGSVSEDFSIMQTPGENFDGKCDSHLSSEDE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
            ESKANL KDSTKSED+I+F DQKSPDSMRRGS GIVG MMLLNS+Q MHAPFTQDVPLM
Sbjct: 541 LESKANLLKDSTKSEDLITFADQKSPDSMRRGSAGIVGNMMLLNSHQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +S PKDE LP+G ATETSKNNW PRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDGSATETSKNNWRPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKAAADTLSQT+YGGLKLMK KMEQLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEV DDLRRLCVVFEHVEKLMALAASLHRK SQAPRLSE IFKDY+DF
Sbjct: 781 LKFLQGNRLSAESEVFDDLRRLCVVFEHVEKLMALAASLHRKFSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTGSSG+KF TEF+KKQ VRSHERG ISSMFTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           +LREIVFSLRDRVNGNHYADSTP AR EE++THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTANDLRVALSVTSCD 954

BLAST of Lcy10g000560 vs. ExPASy TrEMBL
Match: A0A0A0LV89 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_1G533420 PE=3 SV=1)

HSP 1 Score: 1776.9 bits (4601), Expect = 0.0e+00
Identity = 880/954 (92.24%), Postives = 916/954 (96.02%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASS+KVESNGEDEAL+DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNYSMEYYFGN+N+ KVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHDKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSSVDI TN FKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           RADA+MTESA E+A G  GKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKM+ESSLEMA
Sbjct: 241 RADADMTESAEENADGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMIESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITDS+MGNRVGFSSQ+YLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSSQMYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDNLKSSTVVPPPTV+DRVLKELFHEGKK  YFAKGE R SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVVDRVLKELFHEGKKFHYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCLGSQDP+SPR E+DGPVSEDFS+MQTPGENFDGKCDSH S E E
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGPVSEDFSLMQTPGENFDGKCDSH-SSEDE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESKANL +DS+KSED+ISFTDQKSPDSMRRGS GIVG MMLLNSYQ MHAPFTQDVPLM
Sbjct: 541 FESKANLLEDSSKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +S PKDE LP+  ATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDSSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKAAADTLSQT+YGGLKLMK KMEQLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYTTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSE IFKDY+DF
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTGSSG+KF TEF+KKQ VRSHERG ISSMF PPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFRTEFNKKQLVRSHERGVISSMFIPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           +LREIVFSLRDRVNGNHYADSTP A+ EE++THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAQEEEIDTHRMYINGTANDLRVALSVTSCD 953

BLAST of Lcy10g000560 vs. NCBI nr
Match: XP_038894474.1 (rab3 GTPase-activating protein catalytic subunit isoform X3 [Benincasa hispida])

HSP 1 Score: 1793.5 bits (4644), Expect = 0.0e+00
Identity = 885/954 (92.77%), Postives = 917/954 (96.12%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASS+KVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSAKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNYSMEYYFGN+N+GKVVDWEL LHE+QLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHEIQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSS DI TN FKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSADIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           RADA+  ES  E+A+G  GKVQWDDDCPW+EWYS EDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RADADTIESVQENAIGTHGKVQWDDDCPWTEWYSLEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITD+++GNRVGFSSQLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDNTIGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGE R SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPR+P+SSSIDLSTCLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRIPVSSSIDLSTCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCLGSQDP+SPR E+DGPVSEDFS+MQ PGENFDGK DSHLS E E
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGPVSEDFSIMQMPGENFDGKSDSHLSSEDE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESK NL KDSTKSED+ISFTDQKSPDSMRRGS GIVG MMLLNSYQSMHAPFTQDVPLM
Sbjct: 541 FESKVNLLKDSTKSEDLISFTDQKSPDSMRRGSAGIVGNMMLLNSYQSMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           DS P+DE LP+G ATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSGPEDEDLPHGSATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCTAF AAADTLSQTSYGGLKLMK KMEQLYCTM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFNAAADTLSQTSYGGLKLMKTKMEQLYCTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASL+RKLSQAPRLSE IFKDYYDF
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLYRKLSQAPRLSEMIFKDYYDF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTGSSG+KFGTEF+KKQ VR+HERG ISSMFTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFGTEFNKKQLVRNHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           ILREIVFSLRDRVNGNHYADSTP AR EE++THRMYI+GTSNDLRVALSVTSCD
Sbjct: 901 ILREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTSNDLRVALSVTSCD 954

BLAST of Lcy10g000560 vs. NCBI nr
Match: XP_022998101.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucurbita maxima])

HSP 1 Score: 1788.1 bits (4630), Expect = 0.0e+00
Identity = 888/954 (93.08%), Postives = 915/954 (95.91%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASSSK ESNGEDEA+EDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA
Sbjct: 1   MASSSKAESNGEDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADRIGTQVPIKLMHLEGLYELFVSKFAYSS++IS NLFKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           R DAEMTES +ESA+G  GK QWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITD++MGNRVGFSSQLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKSPYF+KGE RTSQAIKAA LDSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCL SQDP+SPRAEEDGPVSED S MQTPGENFDGKCDSH SPEVE
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHSSPEVE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESKANLFK+ TKSEDVISFTDQKS DS RRGS GIVGTMMLLNSYQSMH+PFTQD PLM
Sbjct: 541 FESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHSPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           DSDPKDEGLPNG ATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCTAFKA ADTLSQTSYGGLKLMK KM+QLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSEAIFKDYY+F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTG S NKF TEF+KKQ VRSHERGAISS+FTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           ILREI+FSLRDRV+GNHYADSTP AR E +ETHRMYISGTSNDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Lcy10g000560 vs. NCBI nr
Match: XP_022949104.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucurbita moschata])

HSP 1 Score: 1786.9 bits (4627), Expect = 0.0e+00
Identity = 888/954 (93.08%), Postives = 914/954 (95.81%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASSSK ESNG DEA+EDEEVEHFDDFTIASTWERFISEIEAVCRQWM+DGPNNLLKKDA
Sbjct: 1   MASSSKAESNGGDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMSDGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DIS NLFKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           R DAEMTES +ESA+G  GK QWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITD++MGNRVGFSSQLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKSPYFAKGE RTSQAIKAA LDSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCL SQDP+SPRAEEDGPVSED S MQTPGENFDGKCDSHLSPEVE
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDGKCDSHLSPEVE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESK NLF++ TKSEDVISFTDQKS DS RRGS GIVGTMMLLNSYQSMHAPFTQD PLM
Sbjct: 541 FESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHAPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           DSDPKDEGLPNG ATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCTAFKA ADTLSQTSYGGLKLMK KM+QLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSEAIFKDYY+F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTG S NKF TEF+KKQ VRSHERGAISS+FTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           ILREI+FSLRDRV+GNHYADSTP AR E +ETHRMYISGTSNDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Lcy10g000560 vs. NCBI nr
Match: XP_023522723.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucurbita pepo subsp. pepo] >XP_023522729.1 rab3 GTPase-activating protein catalytic subunit isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1785.4 bits (4623), Expect = 0.0e+00
Identity = 888/954 (93.08%), Postives = 914/954 (95.81%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASSSK ESNGEDEA+EDEEVEHFDDFTIASTWERFISEIEAVCRQWM+DGPNNLLKKDA
Sbjct: 1   MASSSKAESNGEDEAMEDEEVEHFDDFTIASTWERFISEIEAVCRQWMSDGPNNLLKKDA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           T LEISTNLYKVKSDLKCDNKNYSMEYYFGN+ +GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSKHGKVVDWELSLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DIS NLFKVHFTMKLTF+ILLSDDDDIR
Sbjct: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLTFKILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           R DAEM ES +ESA+G  GK QWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RDDAEMAESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITD++MGNRVGFSSQLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDN KSST VPPPTVIDRVLKELFHEGKKSPYFAKGE RTSQAIKAA LDSLFAQF
Sbjct: 361 ENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAIKAAALDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCL SQDP+SPRAEEDGPVSED S MQTPGENFDGKCDSHLSPEVE
Sbjct: 481 ICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDVSTMQTPGENFDGKCDSHLSPEVE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
           FESKANLF++ TKSEDVISFTDQKS DS RRGS GIVGTMMLLNSYQSMHAPFTQD PLM
Sbjct: 541 FESKANLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSMHAPFTQDAPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSP DW E
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPRDW-E 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           DSDPK EGLPNG ATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 DSDPKGEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCTAFKA ADTLSQTSYGGLKLMKAKM+QLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKAKMDQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKL QAPRLSEAIFKDYY+F
Sbjct: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLSEAIFKDYYEF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTG S NKF TEF+KKQ VRSHERGAISS+FTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           ILREI+FSLRDRV+GNHYADSTP AR E +ETHRMYISGTSNDLRVALSVTSCD
Sbjct: 901 ILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALSVTSCD 953

BLAST of Lcy10g000560 vs. NCBI nr
Match: XP_008457337.1 (PREDICTED: rab3 GTPase-activating protein catalytic subunit [Cucumis melo] >KAA0060023.1 rab3 GTPase-activating protein catalytic subunit [Cucumis melo var. makuwa] >TYJ97280.1 rab3 GTPase-activating protein catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 1783.1 bits (4617), Expect = 0.0e+00
Identity = 880/954 (92.24%), Postives = 916/954 (96.02%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MASS+KVESNGEDEAL+DEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKK +
Sbjct: 1   MASSTKVESNGEDEALDDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKGS 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGKVVDWELRLHELQLCFGVQEFLVIA 120
           TQLEISTNLYKVKSDLKCDNKNY+MEYYFGN+N+GKVVDWEL LHELQLCFGVQEFLVIA
Sbjct: 61  TQLEISTNLYKVKSDLKCDNKNYTMEYYFGNSNHGKVVDWELNLHELQLCFGVQEFLVIA 120

Query: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180
           PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF
Sbjct: 121 PQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTFTRRF 180

Query: 181 EADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDDDDIR 240
           EADR+GTQVPIKLMHLEGLYELFVSKFAYSSVDI TN FKVHFTMKLTFRILLSDDDDIR
Sbjct: 181 EADRVGTQVPIKLMHLEGLYELFVSKFAYSSVDIPTNFFKVHFTMKLTFRILLSDDDDIR 240

Query: 241 RADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300
           RAD +M+ESA E+A+G  GKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA
Sbjct: 241 RADTDMSESAQENAVGTHGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKMVESSLEMA 300

Query: 301 ELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQFMEDFVSI 360
           ELENSSPHEAKKWIL PILSPNITDS+MGNRVGFS+QLYLLIDALDMSFQAQFMEDFVS+
Sbjct: 301 ELENSSPHEAKKWILFPILSPNITDSTMGNRVGFSTQLYLLIDALDMSFQAQFMEDFVSV 360

Query: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAADLDSLFAQF 420
           ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGE R SQAIKAA +DSLFAQF
Sbjct: 361 ENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEHRNSQAIKAAPVDSLFAQF 420

Query: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLA 480
           CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS+CLINQKLQMLA
Sbjct: 421 CLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSSCLINQKLQMLA 480

Query: 481 ICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVE 540
           ICIE+KHQSIEEFQDCLGSQDP+SPR E+DG VSEDFS+MQTPGENFDGKCDSHLS E E
Sbjct: 481 ICIERKHQSIEEFQDCLGSQDPESPRDEDDGSVSEDFSIMQTPGENFDGKCDSHLSSEDE 540

Query: 541 FESKANLFKDSTKSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLM 600
            ESKANL KDSTKSED+I+F DQKSPDSMRRGS GIVG MMLLNS+Q MHAPFTQDVPLM
Sbjct: 541 LESKANLLKDSTKSEDLITFADQKSPDSMRRGSAGIVGNMMLLNSHQIMHAPFTQDVPLM 600

Query: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660
           TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE
Sbjct: 601 TEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEE 660

Query: 661 DSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720
           +S PKDE LP+G ATETSKNNW PRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP
Sbjct: 661 ESGPKDEDLPDGSATETSKNNWRPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDP 720

Query: 721 NREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSV 780
           NREGEKILHYLETLRPHQLLEQMVCT+FKAAADTLSQT+YGGLKLMK KMEQLY TM SV
Sbjct: 721 NREGEKILHYLETLRPHQLLEQMVCTSFKAAADTLSQTNYGGLKLMKTKMEQLYSTMASV 780

Query: 781 LKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQAPRLSEAIFKDYYDF 840
           LKFLQGNRLSAESEV DDLRRLCVVFEHVEKLMALAASLHRK SQAPRLSE IFKDY+DF
Sbjct: 781 LKFLQGNRLSAESEVFDDLRRLCVVFEHVEKLMALAASLHRKFSQAPRLSEVIFKDYFDF 840

Query: 841 YLPRMGTGSSGNKFGTEFDKKQQVRSHERGAISSMFTPPTASQSWRRFLSMGNLFNGHEP 900
           YLPRMGTGSSG+KF TEF+KKQ VRSHERG ISSMFTPPTASQSWR+ LSMGNLFNGHEP
Sbjct: 841 YLPRMGTGSSGSKFKTEFNKKQLVRSHERGVISSMFTPPTASQSWRKVLSMGNLFNGHEP 900

Query: 901 ILREIVFSLRDRVNGNHYADSTPKAR-EEVETHRMYISGTSNDLRVALSVTSCD 954
           +LREIVFSLRDRVNGNHYADSTP AR EE++THRMYI+GT+NDLRVALSVTSCD
Sbjct: 901 MLREIVFSLRDRVNGNHYADSTPTAREEEIDTHRMYINGTANDLRVALSVTSCD 954

BLAST of Lcy10g000560 vs. TAIR 10
Match: AT5G58510.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G55060.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 599/977 (61.31%), Postives = 735/977 (75.23%), Query Frame = 0

Query: 1   MASSSKVESNGEDEALEDEEVEHFDDFTIASTWERFISEIEAVCRQWMADGPNNLLKKDA 60
           MAS SK     ED+  E EEV+HFDDFT+AS+WERFIS+IEA CRQW+ADGP NL++K A
Sbjct: 1   MASVSKSNHLEEDDDAE-EEVQHFDDFTLASSWERFISDIEATCRQWLADGPKNLVEKGA 60

Query: 61  TQLEISTNLYKVKSDLKCDNKNYSMEYYFGNNNNGK----VVDWELRLHELQLCFGVQEF 120
             +E S NL+ VK +LK   K+Y ME+YF  +NNG     + +W    H+LQLCFGV++F
Sbjct: 61  VAVEDSKNLFTVKHELKNVAKSYCMEFYFQIDNNGSQQAGIGNWNSNSHDLQLCFGVKDF 120

Query: 121 LVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGIQNMGTTF 180
           L+IAPQS SGV+LD+PE+SKLLSAVAIAL+NC S WPAFVPVHDPSRKAYIGIQNMGT F
Sbjct: 121 LLIAPQSASGVLLDTPESSKLLSAVAIALSNCGSLWPAFVPVHDPSRKAYIGIQNMGTVF 180

Query: 181 TRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISTNLFKVHFTMKLTFRILLSDD 240
           TRRFEADR+G+QVP+KLMHLEGLYELFVSKF YS VD S + F+VHF M+LT++    D+
Sbjct: 181 TRRFEADRVGSQVPVKLMHLEGLYELFVSKFVYSGVDFSMHTFRVHFMMRLTYQTFPYDE 240

Query: 241 D----DIRRADAEMTESAHESALGIRGKVQWDDDCPWSEWYSSEDPVKGFELIASWSEKM 300
           +    D      + +++A       R KV WDDDCPWSEWYS+EDP++GFEL+ +W+++ 
Sbjct: 241 EDEEIDTDEVMGDKSDTAEHYGSESRNKVLWDDDCPWSEWYSAEDPLRGFELVVTWADRT 300

Query: 301 VESSLEMAELENSSPHEAKKWILLPILSPNITDSSMGNRVGFSSQLYLLIDALDMSFQAQ 360
           VES+LEMAELEN+SPH+A+KWIL PILSP + D S G R+ F+SQL  L++ALD SF AQ
Sbjct: 301 VESTLEMAELENASPHDAEKWILHPILSPYLGDPSHGKRIDFASQLLCLVEALDSSFSAQ 360

Query: 361 FMEDFVSIENPGSDNLKSSTVVPPPTVIDRVLKELFHEGKKSPYFAKGEDRTSQAIKAAD 420
           FMEDFVS+ENP S+NLK+S V+PPP+V+DRV+K+LF EG K P F KGE R S+A+KAA 
Sbjct: 361 FMEDFVSVENPSSENLKTSVVIPPPSVLDRVIKDLFREGSKLPDFTKGEHRLSRALKAAP 420

Query: 421 LDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLI 480
           L+SLF QFCLH LWFGNCNIRAIA LW+EFVREVRWCWEE QPLP+MPI  SIDLS+CLI
Sbjct: 421 LESLFTQFCLHSLWFGNCNIRAIAFLWIEFVREVRWCWEETQPLPKMPIDGSIDLSSCLI 480

Query: 481 NQKLQMLAICIEKKHQSIEEFQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCD 540
           NQKL +LAICIEKK +  EEF DC+GS D            S D SV        D + +
Sbjct: 481 NQKLHLLAICIEKKREMNEEFLDCIGSDD------------SSDASVSMEEHHKVDKRRN 540

Query: 541 SHLSPEVEFESKANLFKDSTK------SEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSY 600
           +    E+  +  +++ +D++K        +  +  +Q   D++RRGS G VGTMMLL S 
Sbjct: 541 TSSEEELRRKRDSSIAEDTSKQLRFERKTERTNSVNQSPTDAIRRGSAGPVGTMMLLKSR 600

Query: 601 QSMHAPFTQDVPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVF 660
           Q +HAPFTQD PLMTEDMHEERLQAVEAFGDS +   QLEKDIL SDMSAFKAANPD VF
Sbjct: 601 QQLHAPFTQDPPLMTEDMHEERLQAVEAFGDSLNVPGQLEKDILLSDMSAFKAANPDAVF 660

Query: 661 EDFIRWHSPGDWEEDSDPKDEGLPNGPATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDA 720
           EDFIRWHSPGDW E  +PK      GP+TE SK+ WPPRG LS+RMS+ GNLWR+ WNDA
Sbjct: 661 EDFIRWHSPGDW-ESFEPKTTEPSAGPSTEGSKDEWPPRGRLSQRMSDQGNLWRKSWNDA 720

Query: 721 PALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLM 780
           PALP  +QK LLDPNREGEKI+HYLET+RPHQLLEQMVCTAF+ +ADTL+QT+ G ++ M
Sbjct: 721 PALPADDQKPLLDPNREGEKIVHYLETVRPHQLLEQMVCTAFRGSADTLNQTNVGNMRQM 780

Query: 781 KAKMEQLYCTMVSVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQA 840
            +K+EQLY  M   L  LQ N L  +++ + DL+RLC+VFE+VEKL+A+AAS+HRK   A
Sbjct: 781 TSKLEQLYLIMKFTLGALQRNNLPDKAKTVKDLKRLCMVFENVEKLVAVAASIHRKFLDA 840

Query: 841 PRLSEAIFKDYYDFYLPRMGTGSSG--NKFGTEFD-KKQQVRSHERGAISSMFTPPTASQ 900
            RL++ IF D+Y  Y P MG  ++   NK  TE +  +Q+V   ER  +S++F+PP+A+Q
Sbjct: 841 SRLAQVIFSDFYGVYAPTMGMSANDEENKSRTEMEVSRQEVSLRERQVVSNLFSPPSANQ 900

Query: 901 SWRRFLSMGNLFNGHEPILREIVFSLRDRV-NGNHYADSTPKAR------EEVETHRMYI 954
           SWR+ LSMGNL NGHEPILREI+FS  D V NG HYA +   A       EE+ETHRMY+
Sbjct: 901 SWRKVLSMGNLLNGHEPILREIIFSTGDDVNNGIHYAAAADVAATSDRKGEEIETHRMYV 960

BLAST of Lcy10g000560 vs. TAIR 10
Match: AT5G55060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1); Has 223 Blast hits to 218 proteins in 80 species: Archae - 0; Bacteria - 2; Metazoa - 117; Fungi - 6; Plants - 65; Viruses - 0; Other Eukaryotes - 33 (source: NCBI BLink). )

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-32
Identity = 113/393 (28.75%), Postives = 177/393 (45.04%), Query Frame = 0

Query: 433 RAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLAICIEKKHQSIEE 492
           R +A  W   V E+R  W E + +P +P+ ++ DL +CL++Q LQ++  C+ +K +++  
Sbjct: 261 RRMALFWCRVVEELRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNVAA 320

Query: 493 FQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVEFESKANLFKDST 552
             + L +    +  A E   VS                           ES  +L    +
Sbjct: 321 -SEALDAVMRQASSANEKSDVS---------------------------ESMGSLLYAKS 380

Query: 553 KSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLMTEDMHEERLQAV 612
            S ++I          +R G    V  + +L + + +++P TQ+ PL+TED+  E  + V
Sbjct: 381 NSGELI----------LRLGVYNQVENLTMLETGEPVYSPITQEGPLLTEDLIRETEELV 440

Query: 613 EAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEEDSDPKDEGLPNG 672
              G      +Q     L SDM AFKAANP  + EDF+RWHSP DW E+ +   +     
Sbjct: 441 LRTGSMGAGCSQ-----LLSDMQAFKAANPGCILEDFVRWHSPPDWTENGNMSGD----- 500

Query: 673 PATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLE 732
                  ++ P RG LS RM + GNLWR+LW  A  LP  +Q  L D +   E IL+YLE
Sbjct: 501 -------DSSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYLE 560

Query: 733 TLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSVLKFLQGNRLSAE 792
            +   +L EQ+  +        +         L K   E        V+   QG    A 
Sbjct: 561 DIPAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKLFFE----CKDYVVAICQG---GAW 588

Query: 793 SEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQ 826
           ++ +DD   LC V+E VE ++     + R + Q
Sbjct: 621 TDKLDD---LCQVYETVETMLLRPEEVLRSMKQ 588

BLAST of Lcy10g000560 vs. TAIR 10
Match: AT5G55060.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1). )

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-32
Identity = 113/393 (28.75%), Postives = 177/393 (45.04%), Query Frame = 0

Query: 433 RAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLINQKLQMLAICIEKKHQSIEE 492
           R +A  W   V E+R  W E + +P +P+ ++ DL +CL++Q LQ++  C+ +K +++  
Sbjct: 168 RRMALFWCRVVEELRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNVAA 227

Query: 493 FQDCLGSQDPDSPRAEEDGPVSEDFSVMQTPGENFDGKCDSHLSPEVEFESKANLFKDST 552
             + L +    +  A E   VS                           ES  +L    +
Sbjct: 228 -SEALDAVMRQASSANEKSDVS---------------------------ESMGSLLYAKS 287

Query: 553 KSEDVISFTDQKSPDSMRRGSVGIVGTMMLLNSYQSMHAPFTQDVPLMTEDMHEERLQAV 612
            S ++I          +R G    V  + +L + + +++P TQ+ PL+TED+  E  + V
Sbjct: 288 NSGELI----------LRLGVYNQVENLTMLETGEPVYSPITQEGPLLTEDLIRETEELV 347

Query: 613 EAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIRWHSPGDWEEDSDPKDEGLPNG 672
              G      +Q     L SDM AFKAANP  + EDF+RWHSP DW E+ +   +     
Sbjct: 348 LRTGSMGAGCSQ-----LLSDMQAFKAANPGCILEDFVRWHSPPDWTENGNMSGD----- 407

Query: 673 PATETSKNNWPPRGHLSKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLE 732
                  ++ P RG LS RM + GNLWR+LW  A  LP  +Q  L D +   E IL+YLE
Sbjct: 408 -------DSSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYLE 467

Query: 733 TLRPHQLLEQMVCTAFKAAADTLSQTSYGGLKLMKAKMEQLYCTMVSVLKFLQGNRLSAE 792
            +   +L EQ+  +        +         L K   E        V+   QG    A 
Sbjct: 468 DIPAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKLFFE----CKDYVVAICQG---GAW 495

Query: 793 SEVIDDLRRLCVVFEHVEKLMALAASLHRKLSQ 826
           ++ +DD   LC V+E VE ++     + R + Q
Sbjct: 528 TDKLDD---LCQVYETVETMLLRPEEVLRSMKQ 495