Lcy10g000340 (gene) Sponge gourd (P93075) v1

BLAST of Lcy10g000340 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 9.4e-142
Identity = 304/809 (37.58%), Postives = 486/809 (60.07%), Query Frame = 0

Query: 2   STEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGE 61
           S E + + S +C A     + GV+ GDNP  F   + + QL+L  ++T     IL P  +
Sbjct: 4   SEEPSTDASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQ 63

Query: 62  SSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTL 121
              IS++L G+VLGPS  G +     TIFP +S  V ET A  G + F+FLVGV+MD+ +
Sbjct: 64  PRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMV 123

Query: 122 IGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIA 181
           + K+G +A+ I +   V P LI    +  +  + D  L     ++F+G   S ++F V+A
Sbjct: 124 VRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSED-HLGQGTYILFLGVALSVTAFPVLA 183

Query: 182 CLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICML 241
            +L++LKL+N+++GR+++S+++++   +W+ L +   + + S +   A  W+ +     +
Sbjct: 184 RILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAE-SDKTSFASLWVMISSAVFI 243

Query: 242 IMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGL 301
            + ++++RP + WI+  T   G    E H+  +   ++     ++ +G H + G  + GL
Sbjct: 244 AVCVFVVRPGIAWIIRKT-PEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGL 303

Query: 302 AVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWI-VHLLAFSSCIG 361
            +P+G PLG  L++KL+ FVS ++LP++F +SG + N++     + W+ + L+ F +C G
Sbjct: 304 VIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAG 363

Query: 362 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 421
           K+IGT++ + +  M + + ++LGL+++T+G+ ++++        ++D ++++ MV+  +V
Sbjct: 364 KVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALV 423

Query: 422 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 481
           +TG I+PIV ++Y P KK   S +RRTI+   P  ELR++VC+H   N P+IINLLE S+
Sbjct: 424 MTGVITPIVTILYKPVKK-SVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASH 483

Query: 482 PTIKSPICFYLLHLLQLTGRASPLLI-HHHRPTRRSSLRSN--LSDHIINAFQIYQQFNF 541
           PT +SPIC Y+LHL++LTGRAS +LI H+ R + R +L      SDHIINAF+ Y+Q + 
Sbjct: 484 PTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HA 543

Query: 542 NKVTMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTN-AIRGVNKN 601
             V +   T+++PY+TMH+DVC LA +KRV  +I+PFHK +T++G  ESTN A R VN+N
Sbjct: 544 AFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQN 603

Query: 602 ILSKAPCSVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPM 661
           +L  +PCSVGIL+DR  + +    S  N V L +V ++F  GPDDREALAYA RMA+HP 
Sbjct: 604 LLENSPCSVGILVDRGLNGATRLNS--NTVSL-QVAVLFFGGPDDREALAYAWRMAQHPG 663

Query: 662 VALTVVHLIDPKTNNDW-----------------HDPDQVLDSEMINEFKRIMETTEMKH 721
           + LTV+  I  +   D                  H   + LD + IN F+   E  E + 
Sbjct: 664 ITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFR--AENAEYES 723

Query: 722 CVYKEEMASDCVGLINALRTMEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDML 781
            VY E++ S+    + A+R+M+   D+ +VGR     S L  GL DW+E PELG IGD+L
Sbjct: 724 IVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLL 783

Query: 782 VSSDSTGTATVLVVQQQTIGGDQEFLEDF 789
            SSD   T +VLVVQQ      QE   DF
Sbjct: 784 ASSDFAATVSVLVVQQYVGSWAQEDDMDF 801

BLAST of Lcy10g000340 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 8.0e-125
Identity = 257/770 (33.38%), Postives = 448/770 (58.18%), Query Frame = 0

Query: 12  VCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFISQMLVG 71
           VCQ NH   S+GVF G +P  +   +++ Q+S+  + + LL  +L PL +    +Q+L G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 72  LVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKSGTKAMV 131
           ++LGPS +G++ + ++   P       +T +  G  + +FL+G+++D ++I K+G+KA++
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 132 IGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLSDLKLLN 191
           IG  ++  P  +     LFLKNT ++   + + +  + +L + +SF V   +L++L +LN
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 192 SDVGRLALSSSMISGTLSW-VSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVIYIMRP 251
           SD+GRLA + S++    SW V+LV    +R  ++    A  W  + V  +++++ ++ RP
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFRMFLRDGTL----ASVWSFVWVTALILVIFFVCRP 266

Query: 252 IMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGPPLG 311
            + W+    + S     E   F + ++LL  +L SE +G H   G   LG+++PDGPPLG
Sbjct: 267 AIIWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLG 326

Query: 312 SALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIGKLIGTMLPSL 371
           + L  KL+ F +S+MLP +  +SG + N      + + I+  +   +   K +GT   S 
Sbjct: 327 TGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASA 386

Query: 372 YCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGTISPIVK 431
           YC + + D+ SL L+M  QG+ +I          +++ + ++++++ +++VTG    +V 
Sbjct: 387 YCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVV 446

Query: 432 LIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIKSPICFY 491
            +Y+PSK+Y+   +R  ++      + RL++C+++ +N PS++NLLE S P+  SPI  +
Sbjct: 447 CLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVF 506

Query: 492 LLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNAFTSVAP 551
            LHL++L GRA  +L+ HH+  +     +  S HI+N FQ ++Q N   +    FT+ AP
Sbjct: 507 TLHLVELKGRAHAVLVPHHQMNKLDP-NTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAP 566

Query: 552 YATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTN-AIRGVNKNILSKAPCSVGILI 611
           +++++DD+C LAL+K+  ++++PFHK   I+G  +  N +IR +N N+L KAPCSVGI I
Sbjct: 567 FSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFI 626

Query: 612 DRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVHLIDPKT 671
           DR  +    SV +        V ++F+EG DD EALA++ R+AEHP V++T++H     +
Sbjct: 627 DRGETEGRRSVLM--SYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSS 686

Query: 672 NNDWHDPD---QVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHDCDM 731
               H  D   ++ +S +IN+FK            Y+EE+  D V     + ++    D+
Sbjct: 687 LQQNHVVDVESELAESYLINDFKNF--AMSKPKISYREEIVRDGVETTQVISSLGDSFDL 746

Query: 732 ILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQ 777
           ++VGR HD +S++  GL DW+E PELG IGDM  SSD     +VLV+ QQ
Sbjct: 747 VVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDF--HFSVLVIHQQ 785

BLAST of Lcy10g000340 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.5e-115
Identity = 260/784 (33.16%), Postives = 437/784 (55.74%), Query Frame = 0

Query: 12  VCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFISQMLVG 71
           VCQA +   SRG+F   NP  +   +L+ Q+S+  + + L+  +L PL +    +Q+L G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 72  LVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKSGTKAMV 131
           +VLGPSF G N+  +    P     + +T +  G ++ +FL+G+K+D ++I K+G+KA++
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 132 IGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLSDLKLLN 191
           IG  ++  P  +     +F+  T+ +   + +      +L S +SF V   +L++L +LN
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNILN 208

Query: 192 SDVGRLALSSSMISGTLSW-VSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVIYIMRP 251
           S++GRLA   SM+    SW V+L      R  ++    AL      +I +L+++ ++ RP
Sbjct: 209 SELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTSLYALS----MIIGLLLVIYFVFRP 268

Query: 252 IMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGPPLG 311
           I+ W+      S         F + L+L   +L  E +G H   G   LG+++PDGPPLG
Sbjct: 269 IIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLPDGPPLG 328

Query: 312 SALVDKLDSFVSSVMLPVYFVVSGSRIN---LSKTNMTSIWIVHLLAFSSCIGKLIGTML 371
           + L  KL+ F S++ LP +  +SG + N   +++++   + ++ ++   +   K +GT  
Sbjct: 329 TELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGTAA 388

Query: 372 PSLYCKMSLVDSLSLGLIMSTQGIADI---LIFQHAMLLFMIDQKSYSMMVVAMMVVTGT 431
            S YC+  + D+L L  +M  QGI ++   ++++ A    ++D + ++++++ ++ VTG 
Sbjct: 389 ASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQ---VVDTECFNLVIITILFVTGI 448

Query: 432 ISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIK 491
              +V  +Y+PSK+YK   +R  +       +LRL++ +++ +N PS++NLLE + PT  
Sbjct: 449 SRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRF 508

Query: 492 SPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNA 551
           +PI F+ LHL++L GRA  LL  HH+  +     +  S HI+NAFQ ++Q     +    
Sbjct: 509 NPISFFTLHLVELKGRAHALLTPHHQMNKLDP-NTAQSTHIVNAFQRFEQKYQGALMAQH 568

Query: 552 FTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNA-IRGVNKNILSKAPC 611
           FT+ APY+++++D+C LAL+K+  ++++PFHK   I+G     N  IR +N N+L  APC
Sbjct: 569 FTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPC 628

Query: 612 SVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVH 671
           SV I IDR  +    SV + N      V M+F+ G DD EALA   RMAE P + +T++H
Sbjct: 629 SVAIFIDRGETEGRRSVLMTN--TWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIH 688

Query: 672 LIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHD 731
                   D  D   + +  +I++FK         H  Y EE+  D V     + ++   
Sbjct: 689 FRHKSALQD-EDYSDMSEYNLISDFKSYAANKGKIH--YVEEIVRDGVETTQVISSLGDA 748

Query: 732 CDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGGDQE 788
            DM+LVGR HD +S++  GL DW+E PELG IGDML S D     +VLVV QQ  G D  
Sbjct: 749 YDMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDF--HFSVLVVHQQQ-GDDLL 796

BLAST of Lcy10g000340 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.0e-107
Identity = 252/798 (31.58%), Postives = 449/798 (56.27%), Query Frame = 0

Query: 6   AHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFI 65
           A N +  C A     S GVF GDNP  F   + I Q+ +  +LT +L  +L PL +   I
Sbjct: 2   ATNSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVI 61

Query: 66  SQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKS 125
           ++++ G++LGPS  G + + L  +FP KS  V ET A  G + F+FL G+++D   + ++
Sbjct: 62  AEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRT 121

Query: 126 GTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLS 185
           G KA+ I +     P  +    +  LK T+   ++    LVF+G   S ++F V+A +L+
Sbjct: 122 GKKALGIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILA 181

Query: 186 DLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVI 245
           +LKLL +++GRLA+S++ ++   +W+ L +   +  ++     +L W+ L     +I   
Sbjct: 182 ELKLLTTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSL-WVFLSGCAFVIGAS 241

Query: 246 YIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPD 305
           +I+ PI  WI +   + G  ++E+++ +   ++L C   ++ +G H M G  ++G+ +P 
Sbjct: 242 FIIPPIFRWI-SRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPK 301

Query: 306 GPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIW-IVHLLAFSSCIGKLIG 365
             P   ALV+K++  VS + LP+YFV SG + N++       W ++ L+  ++C GK++G
Sbjct: 302 EGPFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILG 361

Query: 366 TMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGT 425
           T+  SL  K+ + ++++LG +M+T+G+ ++++        +++ +++++MV+  +  T  
Sbjct: 362 TLGVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFI 421

Query: 426 ISPIVKLIYNPSKKYKR--SMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPT 485
            +P+V  +Y P+++ K+    + R +E  +   +LR++ C H   + PS+INLLE S   
Sbjct: 422 TTPVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGI 481

Query: 486 IKSP-ICFYLLHLLQLTGRASP-LLIHHHRPT-----RRSSLRSNLSDHIINAFQIYQQF 545
            K   +C Y LHL +L+ R+S  L++H  R        R  + ++ +D ++ AFQ +QQ 
Sbjct: 482 EKGEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNAD-ADQVVVAFQAFQQ- 541

Query: 546 NFNKVTMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNA-IRGVN 605
             ++V +   T+++  + +H+D+C  A+ K+  +VI+PFHK + ++G  E+T    R VN
Sbjct: 542 -LSRVNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVN 601

Query: 606 KNILSKAPCSVGILIDRASSSSATSVSLVNRVDL-YKVGMVFVEGPDDREALAYAARMAE 665
           + +L +APCSVGI +DR    S    S V+  D+ Y V ++F  GPDDREALAY  RMAE
Sbjct: 602 RRVLLQAPCSVGIFVDRGLGGS----SQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAE 661

Query: 666 HPMVALTVV----------HLIDPKTNNDWHDPDQV----LDSEMINEFKRIMETTEMKH 725
           HP + LTV            +++ + +N+ ++   V     D E+++E ++I    E   
Sbjct: 662 HPGIVLTVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVK 721

Query: 726 CVYK--EEMASDCVGLINALRTMEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGD 776
            V K  E  A D    I  +R      ++ LVGR   G+ AL   + + +E PELG +G 
Sbjct: 722 FVEKQIENAAVDVRSAIEEVRR----SNLFLVGRMPGGEIAL--AIRENSECPELGPVGS 781

BLAST of Lcy10g000340 vs. ExPASy Swiss-Prot
Match: Q9M007 (Cation/H(+) antiporter 27 OS=Arabidopsis thaliana OX=3702 GN=CHX27 PE=2 SV=2)

HSP 1 Score: 374.4 bits (960), Expect = 3.3e-102
Identity = 243/769 (31.60%), Postives = 416/769 (54.10%), Query Frame = 0

Query: 11  WVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFISQMLV 70
           W+C+      S G+  G+NP  F   +L+ Q+S+ S+ +   Q +L P G+ +F++QML 
Sbjct: 15  WLCEPWVGAGSLGIGRGENPLKFALPLLLLQISVFSIFSVSFQFLLRPFGKFAFLTQMLA 74

Query: 71  GLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKSGTKAM 130
           G+ LGPS  G N   + T F  +S Y+ E+F     +   ++   ++D  +I + G  A 
Sbjct: 75  GICLGPSVIGRNKQYMATFFYARSVYIIESFEAICFLFICYITTCQVDTRMIKRVGKLAF 134

Query: 131 VIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVF--IGALQSSSSFHVIACLLSDLK 190
           + G++ F+ P +   F  + +   +         + F  +  +QS+  F V+  +LS LK
Sbjct: 135 INGILLFLIPFVWGQFAAILISKRLKSGPAGIPPVEFHHVAIVQSTMFFQVVYGVLSSLK 194

Query: 191 LLNSDVGRLALSSSMISGTLSWVSLVVGFTMR-QTSIEEQDALPWMTLCVICMLIMVIYI 250
           +LN++ GRLAL+S M+   LSW   ++   ++    +  ++   ++++  + M++++ Y+
Sbjct: 195 MLNTEPGRLALASMMVHDCLSWCFFMLNIAIKLNVDLPNKNRAAFLSVLQMIMILVIAYV 254

Query: 251 MRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGP 310
            RP+M W+   T   G ++K S++  + ++L    L++EFVG  +  G ++LGLA P  P
Sbjct: 255 FRPLMLWMKNRT-PEGHSLKASYLSVICVLLFISCLWAEFVGLPYFFGAVVLGLATPKRP 314

Query: 311 PLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIGKLIGTML 370
           PLG+ L DK+  FV SV++P Y +  G  I+LS  +   +    LL       K+I   L
Sbjct: 315 PLGTGLSDKIGCFVWSVLMPCYVIGIGLNIDLSLFSWRDVIRFELLFGVVRFAKMIAIAL 374

Query: 371 PSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGTISP 430
           PSLY K+ L  ++ +G I++ QG+ D+ I++       I  KS+  MV++  V +     
Sbjct: 375 PSLYYKVPLWHAILVGFIVNIQGLYDVQIYKQNFNYTKISSKSFGAMVMSATVNSTIFIV 434

Query: 431 IVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIKSPI 490
           IVK +Y    K +   +RRT++H      LR++ C  +++    +++L+E+S P I SP+
Sbjct: 435 IVKKLYQTMSK-RNPYKRRTVQHCRVEAPLRILTCFRNREAVRPVLDLVELSRPAIGSPL 494

Query: 491 CFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNAFTS 550
             + ++L +L   + PLLIHH +      + S   D I+ AF  +++ N   V +  FT+
Sbjct: 495 SVFAVNLEELNNHSLPLLIHHTQEISPFLVPSR-RDQIVKAFHNFEKTNQETVLIECFTA 554

Query: 551 VAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSKAPCSVGI 610
           VAP  TMH+DVC +A ++   +VI+         GIE      R + +N+L   PCSV +
Sbjct: 555 VAPRKTMHEDVCAIAFDQETDIVILTLDA-----GIELWE---RLLCRNLLHNCPCSVAL 614

Query: 611 LIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVHLIDP 670
            IDR        V L        +G +F+ GPDDRE LAYA R+A HP V L V  L+D 
Sbjct: 615 FIDRGRLPDFRFVPLKKLT--INIGAIFLGGPDDREMLAYATRLASHPSVELQVFRLVDQ 674

Query: 671 KTNNDWHD-PDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHDCDM 730
              +   D  ++  D  +IN F++  E +E K+ +++E    + V L++ LR    D D+
Sbjct: 675 NGVSPLRDMVERNHDMRVINVFRK--ENSE-KNIIFREVRIEEAVNLLDLLRKEGDDFDL 734

Query: 731 ILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQ 776
           ++VG RH+ +  +  GL++W++  ELG +GD+L+S D   + +VL VQQ
Sbjct: 735 MMVGIRHEENLLMLEGLSEWSDMKELGEVGDVLISKDLELSVSVLAVQQ 767

BLAST of Lcy10g000340 vs. ExPASy TrEMBL
Match: A0A0A0LT09 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G025970 PE=4 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 640/814 (78.62%), Postives = 722/814 (88.70%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AAHNGSWVCQ    YRSRG+F+GD+PFSF   +L+AQLSLSS LTSLLQC+LTPLG
Sbjct: 1   MSTQAAHNGSWVCQPTTYYRSRGIFFGDSPFSFAKTILLAQLSLSSFLTSLLQCLLTPLG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL LGPSFYG +  IL+ IFP+KSFYV+ETFA+FGCMVFMFLVGVKMDL+
Sbjct: 61  ESSFISQMLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           LI KSG KAMVIG MAF +PML NFF++ +LK+TV+MD HLKN+L  IGA Q+SSSFHVI
Sbjct: 121 LIQKSGKKAMVIGFMAFSTPMLFNFFLSTYLKSTVEMDAHLKNTLSAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           ACLL+DLKLLNSD+GRLALSSSMISGTL W  LV+GFT+RQTS+++QDALPWM LC++CM
Sbjct: 181 ACLLTDLKLLNSDIGRLALSSSMISGTLCWGGLVIGFTLRQTSMQQQDALPWMALCLVCM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+V+YI+RPIMFWI+  TN SGR +KE +VF +FLMLLFC+LFSEFVGQHFMLGPMILG
Sbjct: 241 MILVVYILRPIMFWIIEQTNLSGRPIKEVYVFCLFLMLLFCSLFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLGSALVDKLDSFVSS+MLP YFV+SG+RINLS  N+ S  I++LLAF++ IG
Sbjct: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCYFVISGARINLSTVNVRSAVIINLLAFTAFIG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           K+IGTMLPSLYCKMSLVDSLSLGLIMSTQGIADIL  QH +LL+MIDQKSYSMMVVAMMV
Sbjct: 361 KVIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILGLQHGLLLYMIDQKSYSMMVVAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           +TGTI PIVK+IYNPSKKY+  MRRRTIEH S TGELRL++CIHHQDN PSIIN+LE+SN
Sbjct: 421 MTGTICPIVKMIYNPSKKYRCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPICFYL+HLLQLTGRASPLLI+HH P RR S R NLSD IINAFQIYQQFN++KV
Sbjct: 481 PTIKSPICFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
            MNAFTSV+PYATMHDDVCMLALEKRV MVIVPFHK RTINGI ES N IRGVNKNILSK
Sbjct: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINQIRGVNKNILSK 600

Query: 601 APCSVGILIDRASSSSA-TSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVAL 660
           APCSVGILIDR  S SA +SVSL NRVDLYKVGM+FVEGPDDREALAYA RMAEHP VAL
Sbjct: 601 APCSVGILIDRVLSPSAVSSVSLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVAL 660

Query: 661 TVVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRT 720
           TVV +I+PK ++  H  DQ LD+EMI EFK IM T+E KH  Y+EE+ASDCVGLIN +RT
Sbjct: 661 TVVRVIEPKRSSR-HPTDQDLDAEMIKEFKLIMATSENKHWTYEEEIASDCVGLINVIRT 720

Query: 721 MEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIG 780
           MEHD D+ILVGRRHDGDSALF+GLN+WNE+PELG+IGDML SSDS+G   VLV+QQQTIG
Sbjct: 721 MEHDSDLILVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQQTIG 780

Query: 781 GDQEFLEDFRCLMEESFSVDIKPLKLPESWSTKS 814
           GDQEFL+DFRCLMEESFSVDI PL LP +W  KS
Sbjct: 781 GDQEFLDDFRCLMEESFSVDINPLNLPTAWPQKS 813

BLAST of Lcy10g000340 vs. ExPASy TrEMBL
Match: A0A6J1GBQ8 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111452529 PE=4 SV=1)

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 599/802 (74.69%), Postives = 699/802 (87.16%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AA NGSWVCQ +  Y+SRG+F+GD PFS+GS++L+AQLSLSSLLTSLLQCILTPLG
Sbjct: 1   MSTQAAQNGSWVCQPSSNYQSRGIFFGDTPFSYGSSILLAQLSLSSLLTSLLQCILTPLG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL+LGPSFYG +  +L+ IFP+KSFYV+ETFAFFGC+VFMFLVGVKMDLT
Sbjct: 61  ESSFISQMLVGLMLGPSFYGGDSPVLEAIFPFKSFYVSETFAFFGCLVFMFLVGVKMDLT 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           +I ++G KAMVIGV+AF+SP+ INF +  +LKN++DMDL LKNSL  IGA Q+SSSFHVI
Sbjct: 121 IIQRTGRKAMVIGVLAFISPLFINFILASYLKNSIDMDLQLKNSLTAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           AC L+D+ LLNSD+GRLALSSSMISG LSW ++VV FT++QTS  +QDALPWM LC++CM
Sbjct: 181 ACYLADINLLNSDIGRLALSSSMISGMLSWFAIVVVFTVQQTSNRQQDALPWMALCIVCM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+VIYI+RPIM WI+  TNNSGR +KE +V  +FLMLLFCALFSEFVGQHFMLGPMILG
Sbjct: 241 IILVIYILRPIMLWIIEQTNNSGRVIKEGYVLLVFLMLLFCALFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLG+ALVDKLDSFVSSVMLP YFV+SG+RINLS+ NM S WIV +LA  S +G
Sbjct: 301 LAVPDGPPLGTALVDKLDSFVSSVMLPCYFVISGARINLSEINMRSAWIVQVLALGSFLG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIGT+LPSLYCKM LVDSLSLGLIMSTQGIADIL+ QHAMLLFMIDQKSY+MMVVAMMV
Sbjct: 361 KLIGTVLPSLYCKMPLVDSLSLGLIMSTQGIADILVLQHAMLLFMIDQKSYAMMVVAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           VTG ISPIVK+IYNPSKKYK + RRRTIEHASP+GELRL+VCIHH DN PSIINLLEVSN
Sbjct: 421 VTGMISPIVKIIYNPSKKYKSTSRRRTIEHASPSGELRLLVCIHHHDNTPSIINLLEVSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PT+KSPI FYLLHLLQLTGRASPL I H   +   S R N    IINAFQIYQ++N++KV
Sbjct: 481 PTLKSPIYFYLLHLLQLTGRASPLFIDHQNGSHHDSQRFN---PIINAFQIYQKYNYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
           +MNAFTS+APY +MHDDVCMLALEKRV MVIVPFH+WR +NGI +S N IRGVNKNILSK
Sbjct: 541 SMNAFTSIAPYVSMHDDVCMLALEKRVAMVIVPFHEWRAVNGIMDSMNQIRGVNKNILSK 600

Query: 601 APCSVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALT 660
           APCSVGILIDR S  S   +S++NRVDLYKVGM+FVEG DDREALAYA RMAEHPMVALT
Sbjct: 601 APCSVGILIDRVSLQSTAMMSMMNRVDLYKVGMLFVEGADDREALAYATRMAEHPMVALT 660

Query: 661 VVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTM 720
           VV L+ P++  +    + +LDSEM+NEFKRIMET+ ++HCVY+EEM  DCVGLIN +R+M
Sbjct: 661 VVRLMLPESKTE-RRAELILDSEMLNEFKRIMETSGIQHCVYEEEMVKDCVGLINVIRSM 720

Query: 721 EHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGG 780
           E + D++LVGRRHDG+S LF+GLN+WNE+PELG IGDM  S+D +G   +LVVQQQTIG 
Sbjct: 721 EQNYDLMLVGRRHDGESELFLGLNEWNEYPELGIIGDMFASTDFSGAVAMLVVQQQTIGN 780

Query: 781 DQEFLEDFRCLMEESFSVDIKP 803
           D++ L+DFRCLMEESF+VDIKP
Sbjct: 781 DEDLLDDFRCLMEESFNVDIKP 798

BLAST of Lcy10g000340 vs. ExPASy TrEMBL
Match: A0A6J1HJT4 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111464735 PE=4 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 588/815 (72.15%), Postives = 690/815 (84.66%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           M+  AAHNGSWVCQ N  YRSRG+F+GDNPFSFG+ V +AQ+S+SSLLT LLQC+LTP+G
Sbjct: 1   MNIRAAHNGSWVCQPNERYRSRGIFFGDNPFSFGNTVFLAQISVSSLLTCLLQCLLTPIG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSF SQMLVGLVLGPSF G N  +L+ +FPYKSFYV+ETFAFFGCM+FMFL+GVKMDLT
Sbjct: 61  ESSFFSQMLVGLVLGPSFNGGNSPLLEVVFPYKSFYVSETFAFFGCMIFMFLMGVKMDLT 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           LI KSGTKAMVIGV+ F+ P+LIN  ++++LK+T+DMD +LK+SL  IGA QSSSSF+VI
Sbjct: 121 LITKSGTKAMVIGVLVFLFPLLINCLLSVYLKSTIDMDSNLKDSLTAIGAFQSSSSFYVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           AC+L DLKLLNS +GRLALSSSMISGTLSW+S+V+ FT+RQTS+E+QDALPWM +CV CM
Sbjct: 181 ACVLEDLKLLNSHIGRLALSSSMISGTLSWISIVICFTLRQTSMEQQDALPWMAICVACM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+VIYI+RPIMFWI+  TN SG  +KES+VF MF+M+LFC+LFSEFVGQHF+LGPMILG
Sbjct: 241 IILVIYILRPIMFWIVEQTNISGGPIKESYVFLMFIMMLFCSLFSEFVGQHFLLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLGSALVDKL+SFVSS+MLP YFV+SG+RINLSK +M SIW+V LLAF S +G
Sbjct: 301 LAVPDGPPLGSALVDKLESFVSSIMLPFYFVISGARINLSKLDMKSIWVVQLLAFGSLMG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIG  LPSLYCKM LVDSL+LGLIMSTQGI+DIL  Q  +LL MID  SYS+MV+AMMV
Sbjct: 361 KLIGATLPSLYCKMPLVDSLTLGLIMSTQGISDILFLQQGLLLQMIDLNSYSVMVLAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           +TG  SPIVK++Y PS KY  + RRRTIEH SP GELR+++CIHHQDN PS+INLLEVSN
Sbjct: 421 MTGATSPIVKMLYKPSNKYTCNRRRRTIEHTSPNGELRVLLCIHHQDNTPSVINLLEVSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPICFYL+HL+QLTGRASPLLIHHH P RRSS R NLSD IINAFQ++QQ +++KV
Sbjct: 481 PTIKSPICFYLIHLIQLTGRASPLLIHHH-PNRRSSKRCNLSDQIINAFQLFQQMSYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
            MNAFTSVAP+ATMHDDVCMLALEKRV MVIVPFHK RT+N  E S NAI  VNKNILSK
Sbjct: 541 IMNAFTSVAPFATMHDDVCMLALEKRVSMVIVPFHKRRTMNAYEGSVNAISAVNKNILSK 600

Query: 601 APCSVGILID-RASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVAL 660
           APCSVGILID    +++A +V ++NR +LYKVG++FV G DDREALAYA RM EHPMV L
Sbjct: 601 APCSVGILIDPMIMATTANTVPIMNRAELYKVGLIFVGGSDDREALAYATRMVEHPMVTL 660

Query: 661 TVVHLIDPKTNNDWHDP-DQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALR 720
           TVV LI PK     H P DQ  D EM+NEFK IM ++ +KHC Y+E  ASDCVGLI  +R
Sbjct: 661 TVVRLIHPKGTT--HQPADQEHDFEMLNEFKLIMSSSGIKHCFYEEVTASDCVGLIGVMR 720

Query: 721 TMEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTI 780
            MEH+ D+ILVGRRHDGDSALF+GLN+W+E+PELG+IGDML + DS   ATVLVVQQQTI
Sbjct: 721 KMEHNYDLILVGRRHDGDSALFVGLNEWHEYPELGFIGDMLATIDSPSDATVLVVQQQTI 780

Query: 781 GGDQEFLEDFRCLMEESFSVD-IKPLKLPESWSTK 813
            GD E LEDFRCLM+ESF ++ +KPL    SW  K
Sbjct: 781 VGDHELLEDFRCLMDESFPMEVVKPLNSSNSWHHK 812

BLAST of Lcy10g000340 vs. ExPASy TrEMBL
Match: A0A6J1I5C0 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111469382 PE=4 SV=1)

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 587/811 (72.38%), Postives = 691/811 (85.20%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MS  AAHNGSWVCQ N  YRSRG+F+GDNPFSFG+ V +AQ+S+SSLLT LLQC+LTP+G
Sbjct: 1   MSIRAAHNGSWVCQPNERYRSRGIFFGDNPFSFGNTVFLAQISVSSLLTCLLQCLLTPIG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSF SQMLVGL LGPSF G N  +L+ +FPYKSFYV+ETFAFFGCM+FMFL+GVKMDLT
Sbjct: 61  ESSFFSQMLVGLALGPSFNGGNSPLLEVVFPYKSFYVSETFAFFGCMIFMFLMGVKMDLT 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           LI KSGTKAMVIGV+ F+ P+LIN+ ++++LK+T+DMD +LK+SL  IGA QSSSSF+VI
Sbjct: 121 LITKSGTKAMVIGVLVFLFPLLINYLLSVYLKSTIDMDGNLKDSLTAIGAFQSSSSFYVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           AC+L DL LLNS++GRLALSSSMISGTLSW+S+V+ FT+RQTS+E+QDALPWM +CV CM
Sbjct: 181 ACVLEDLNLLNSNIGRLALSSSMISGTLSWISIVICFTLRQTSLEQQDALPWMAVCVACM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+VIYI+RPIMFWI+  TN SGR +KES+VF MF+M+LFC+LFSEFVGQHF+LGPMILG
Sbjct: 241 IILVIYILRPIMFWIVEQTNISGRPIKESYVFLMFIMMLFCSLFSEFVGQHFLLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLGSALVDKL+SFVSS+MLP YFV+SG+RINLSK +M SIWIV LLAF S +G
Sbjct: 301 LAVPDGPPLGSALVDKLESFVSSIMLPCYFVISGARINLSKLDMKSIWIVQLLAFGSLMG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIG  LPSLYCKM LVDSL+LGLIMSTQGI+DIL  Q  +LL M+D  +YS+MV+AMMV
Sbjct: 361 KLIGATLPSLYCKMPLVDSLTLGLIMSTQGISDILFLQQGLLLQMLDVNAYSVMVLAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           +TG  SPIVK++YNPS KY+ + RRRTIEH SP GELR+++CIHHQDN PSIINLLEVSN
Sbjct: 421 MTGATSPIVKMLYNPSNKYRCNRRRRTIEHTSPNGELRVLLCIHHQDNTPSIINLLEVSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPICFYL+HL+QLTGRASPLLIHHH P+RRSS R NLSD IINAFQ++QQ +++KV
Sbjct: 481 PTIKSPICFYLIHLIQLTGRASPLLIHHH-PSRRSSKRCNLSDQIINAFQLFQQMSYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
            MNAFTSVAP+ATMHDDVCMLALEKRV MVIVPFHK RT+N  E S NAIR VNKNILSK
Sbjct: 541 IMNAFTSVAPFATMHDDVCMLALEKRVSMVIVPFHKRRTMNVYEGSVNAIRAVNKNILSK 600

Query: 601 APCSVGILID-RASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVAL 660
           APCSVGILID     ++A +V ++NRV+LYKVG++FV G DDREALAYA RM EHPMV L
Sbjct: 601 APCSVGILIDPMILPTTANTVPIMNRVELYKVGLIFVGGSDDREALAYATRMVEHPMVTL 660

Query: 661 TVVHLIDPKTNNDWHDP-DQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALR 720
           TVV LI PK     H P DQ  D EM+NEFK IM ++E+KHC Y+E  ASDCVGLI  +R
Sbjct: 661 TVVRLIHPKGTT--HQPADQEHDFEMLNEFKLIMSSSEIKHCFYEEVTASDCVGLIGVMR 720

Query: 721 TMEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTI 780
            ME + D+ILVGRRHDGDS LF+GLN+W+E+PELG+IGDML + DS   ATVLV+QQQTI
Sbjct: 721 KMESNYDLILVGRRHDGDSELFVGLNEWHEYPELGFIGDMLATIDSPSDATVLVIQQQTI 780

Query: 781 GGDQEFLEDFRCLMEESFSVD-IKPLKLPES 809
            GD E LEDFRCLM+ESF  + +KPL    S
Sbjct: 781 VGDHELLEDFRCLMDESFPTEVVKPLNSSNS 808

BLAST of Lcy10g000340 vs. ExPASy TrEMBL
Match: A0A1S3C4R5 (cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103497021 PE=4 SV=1)

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 580/746 (77.75%), Postives = 657/746 (88.07%), Query Frame = 0

Query: 68  MLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKSGT 127
           MLVGL LGPSFYG +  IL+ IFP+KSFYV+ETFA+FGCMVFMFLVG+KMDL+LI KSG 
Sbjct: 1   MLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGIKMDLSLIKKSGK 60

Query: 128 KAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLSDL 187
           KAMVIG MAF++PML NFF++ +LK++V+MD HLKN+L  IGA Q+SSSFHVIACLL+DL
Sbjct: 61  KAMVIGFMAFLTPMLFNFFLSTYLKSSVEMDAHLKNTLNAIGAFQASSSFHVIACLLTDL 120

Query: 188 KLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVIYI 247
           KLLNSD+GRLALSSSMISG LSW  LV+GFT+RQTS+++QDALPWM LC++CM+I+VIYI
Sbjct: 121 KLLNSDIGRLALSSSMISGALSWAGLVIGFTLRQTSMQQQDALPWMALCLVCMMILVIYI 180

Query: 248 MRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGP 307
           +RPIMFWI+  TN SGR +KE +VF +FLMLLFCALFSEFVGQHF+LGPMILGLAVPDGP
Sbjct: 181 LRPIMFWIVEQTNLSGRPIKEVYVFCLFLMLLFCALFSEFVGQHFLLGPMILGLAVPDGP 240

Query: 308 PLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIGKLIGTML 367
           PLGSALVDKLDSFVSS+MLP YFV+SG+RINLS  N+ S  I++LLAF++ IGK+IGTML
Sbjct: 241 PLGSALVDKLDSFVSSIMLPCYFVISGARINLSTINVRSAVIINLLAFTAFIGKVIGTML 300

Query: 368 PSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGTISP 427
           PSLYCKMSLVDSLSLGLIMSTQGIADIL  QH +LLFMIDQ SYSMMVVAMMV+TGTI P
Sbjct: 301 PSLYCKMSLVDSLSLGLIMSTQGIADILSLQHGLLLFMIDQTSYSMMVVAMMVMTGTICP 360

Query: 428 IVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIKSPI 487
           IVK+IYNPSKKYK  MRRRTIEH S TGELRL++CIHHQDN PSIIN+LE+SNPTIKSPI
Sbjct: 361 IVKMIYNPSKKYKCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSNPTIKSPI 420

Query: 488 CFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNAFTS 547
           CFYL+HLLQLTGRASPLLI+HH P RR S R NLSD IINAFQIYQQFN++KV MNAFTS
Sbjct: 421 CFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKVIMNAFTS 480

Query: 548 VAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSKAPCSVGI 607
           V+PYATMHDDVCMLALEKRV MVIVPFH  RT NGI ES N IRGVNKNILSKAPCSVGI
Sbjct: 481 VSPYATMHDDVCMLALEKRVAMVIVPFHNRRTFNGIVESINQIRGVNKNILSKAPCSVGI 540

Query: 608 LIDRASSSSA-TSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVHLID 667
           LIDR    SA  S+SL NRVDLYKVGM+FVEGPDDREALAYA RMAEHP VALTVV +I+
Sbjct: 541 LIDRVMLPSAVASISLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVALTVVRVIE 600

Query: 668 PKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHDCDM 727
           PK     H  DQ LD+EMI EFK IM T+ +KHC Y+EE+A+DCVGLIN +RTMEHD D+
Sbjct: 601 PKQTR--HPADQDLDAEMIKEFKLIMATSGIKHCSYEEEIANDCVGLINVIRTMEHDYDL 660

Query: 728 ILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGGDQEFLE 787
           ILVGRRHDGDSALF+GLN+WNE+PELG+IGDML SSDS+G   VLV+QQQTIGGDQEFL+
Sbjct: 661 ILVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQQTIGGDQEFLD 720

Query: 788 DFRCLMEESFSVDIKPLKLPESWSTK 813
           DF+CLMEESFSV+I PL LP +W  K
Sbjct: 721 DFQCLMEESFSVEINPLNLPGAWPQK 744

BLAST of Lcy10g000340 vs. NCBI nr
Match: XP_004137561.1 (cation/H(+) antiporter 15 [Cucumis sativus] >KGN63917.1 hypothetical protein Csa_013943 [Cucumis sativus])

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 640/814 (78.62%), Postives = 722/814 (88.70%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AAHNGSWVCQ    YRSRG+F+GD+PFSF   +L+AQLSLSS LTSLLQC+LTPLG
Sbjct: 1   MSTQAAHNGSWVCQPTTYYRSRGIFFGDSPFSFAKTILLAQLSLSSFLTSLLQCLLTPLG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL LGPSFYG +  IL+ IFP+KSFYV+ETFA+FGCMVFMFLVGVKMDL+
Sbjct: 61  ESSFISQMLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           LI KSG KAMVIG MAF +PML NFF++ +LK+TV+MD HLKN+L  IGA Q+SSSFHVI
Sbjct: 121 LIQKSGKKAMVIGFMAFSTPMLFNFFLSTYLKSTVEMDAHLKNTLSAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           ACLL+DLKLLNSD+GRLALSSSMISGTL W  LV+GFT+RQTS+++QDALPWM LC++CM
Sbjct: 181 ACLLTDLKLLNSDIGRLALSSSMISGTLCWGGLVIGFTLRQTSMQQQDALPWMALCLVCM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+V+YI+RPIMFWI+  TN SGR +KE +VF +FLMLLFC+LFSEFVGQHFMLGPMILG
Sbjct: 241 MILVVYILRPIMFWIIEQTNLSGRPIKEVYVFCLFLMLLFCSLFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLGSALVDKLDSFVSS+MLP YFV+SG+RINLS  N+ S  I++LLAF++ IG
Sbjct: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCYFVISGARINLSTVNVRSAVIINLLAFTAFIG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           K+IGTMLPSLYCKMSLVDSLSLGLIMSTQGIADIL  QH +LL+MIDQKSYSMMVVAMMV
Sbjct: 361 KVIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILGLQHGLLLYMIDQKSYSMMVVAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           +TGTI PIVK+IYNPSKKY+  MRRRTIEH S TGELRL++CIHHQDN PSIIN+LE+SN
Sbjct: 421 MTGTICPIVKMIYNPSKKYRCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPICFYL+HLLQLTGRASPLLI+HH P RR S R NLSD IINAFQIYQQFN++KV
Sbjct: 481 PTIKSPICFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
            MNAFTSV+PYATMHDDVCMLALEKRV MVIVPFHK RTINGI ES N IRGVNKNILSK
Sbjct: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINQIRGVNKNILSK 600

Query: 601 APCSVGILIDRASSSSA-TSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVAL 660
           APCSVGILIDR  S SA +SVSL NRVDLYKVGM+FVEGPDDREALAYA RMAEHP VAL
Sbjct: 601 APCSVGILIDRVLSPSAVSSVSLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVAL 660

Query: 661 TVVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRT 720
           TVV +I+PK ++  H  DQ LD+EMI EFK IM T+E KH  Y+EE+ASDCVGLIN +RT
Sbjct: 661 TVVRVIEPKRSSR-HPTDQDLDAEMIKEFKLIMATSENKHWTYEEEIASDCVGLINVIRT 720

Query: 721 MEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIG 780
           MEHD D+ILVGRRHDGDSALF+GLN+WNE+PELG+IGDML SSDS+G   VLV+QQQTIG
Sbjct: 721 MEHDSDLILVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQQTIG 780

Query: 781 GDQEFLEDFRCLMEESFSVDIKPLKLPESWSTKS 814
           GDQEFL+DFRCLMEESFSVDI PL LP +W  KS
Sbjct: 781 GDQEFLDDFRCLMEESFSVDINPLNLPTAWPQKS 813

BLAST of Lcy10g000340 vs. NCBI nr
Match: XP_038895476.1 (LOW QUALITY PROTEIN: cation/H(+) antiporter 15-like [Benincasa hispida])

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 633/810 (78.15%), Postives = 715/810 (88.27%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AAHNGSWVCQ N  YRSRG+F+GD+PFSF  +VL+AQLSLSSLLTSLLQC+LTPLG
Sbjct: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL LGPSFYG +  IL+ IFP+KSFYV+ETFA+FGCMVFMFLVGVKMDL+
Sbjct: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           LI KSG KA+VIGV+AFV+PM+ NF +T +LK+ V+MD +LKN L  IGA  +SSSFHVI
Sbjct: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           ACLLSDLKLLNSD+GRLALSSSMISGTLSW+ LV  FTMRQTS+++QD LPWM LCV+CM
Sbjct: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+V+YI+RPIM WIM  TN SGR +KE++V S+FLMLLFCALFSEFVGQHF+LGPMILG
Sbjct: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLGSALVDKLDSFVSS+MLP +FV+SG+R+NLS  +M S  I+HLLAF++ +G
Sbjct: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIGTMLPSLYCKMSLVDSLSLGLIMS QGIADIL  QH +LL+MIDQ SYSM VVAMMV
Sbjct: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           +TGTI PIVK++YNPSK+Y+ S RRRTIEH S   ELRL++CIHHQD+ PSIIN+LE+SN
Sbjct: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPICFYL+HLLQLTGRASPLLIHHH   +R S    LSD II AFQIYQQFN++KV
Sbjct: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
            MNAFTSV+PYATMHDDVCMLALEKRV MVIVPFHK RTINGI ES N IRGVNKNILSK
Sbjct: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSK 600

Query: 601 APCSVGILIDRAS-SSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVAL 660
           APCSVGILIDR    S+A SVSL+NRVDLYKVGM+FVEGPDDREALAYA RMAEHPMVAL
Sbjct: 601 APCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVAL 660

Query: 661 TVVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRT 720
           TVV +I PK ++  H  DQ LD+EMINEFK IMET+ +KHC Y+EEMASDCVGLIN +RT
Sbjct: 661 TVVRVIQPKRSSR-HPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRT 720

Query: 721 MEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIG 780
           MEHD DMILVGRRHDGDSALF+GL++WNEFPELGYIGDML SSDSTG   VLVVQQQTIG
Sbjct: 721 MEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQQTIG 780

Query: 781 GDQEFLEDFRCLMEESFSVDIKPLKLPESW 810
           GDQEFL+DFRCLMEESFSVDI+PL LP +W
Sbjct: 781 GDQEFLDDFRCLMEESFSVDIRPLNLPTAW 809

BLAST of Lcy10g000340 vs. NCBI nr
Match: KAG6607348.1 (Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 604/802 (75.31%), Postives = 700/802 (87.28%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AA NGSWVCQ +  Y+SRG+F+GD PFS+GS++L+AQLSLSSLLT+LL CILTPLG
Sbjct: 6   MSTQAAQNGSWVCQPSSNYQSRGIFFGDTPFSYGSSILLAQLSLSSLLTTLLHCILTPLG 65

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL+LGPSFYGE+  +L+ IFP+KSFYV+ETFAFFGC+VFMFLVGVKMDLT
Sbjct: 66  ESSFISQMLVGLMLGPSFYGEDSPVLEAIFPFKSFYVSETFAFFGCLVFMFLVGVKMDLT 125

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           +I ++G KAM IGV+AF+SP+ INF +  +LKN++DMDL LKNSL  IGA Q+SSSFHVI
Sbjct: 126 VIQRTGRKAMAIGVLAFISPLFINFILASYLKNSIDMDLQLKNSLTAIGAFQASSSFHVI 185

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           AC L+D+ LLNSD+GRLALSSSMISG LSW ++VV FT++QTS  +QDALPWM LC++CM
Sbjct: 186 ACYLADINLLNSDIGRLALSSSMISGMLSWFAIVVAFTVQQTSNRQQDALPWMALCMVCM 245

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+VIYI+RPIM WI+  TNNSGR +KE +V  +FLMLLFCALFSEFVGQHFMLGPMILG
Sbjct: 246 IILVIYILRPIMLWIIEQTNNSGRVIKEGYVLLVFLMLLFCALFSEFVGQHFMLGPMILG 305

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLG+ALVDKLDSFVSSVMLP YFV+SG+RINLS+ NM S WIV +LA  S +G
Sbjct: 306 LAVPDGPPLGTALVDKLDSFVSSVMLPCYFVISGARINLSEINMRSAWIVQVLALGSFLG 365

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIGT+LPSLYCKM LVDSLSLGLIMSTQGIADILI QHAMLLFMIDQKSY+MMVVAMMV
Sbjct: 366 KLIGTVLPSLYCKMPLVDSLSLGLIMSTQGIADILILQHAMLLFMIDQKSYAMMVVAMMV 425

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           VTGTISPIVK+IYNPSKKYK + RRRTIEHASP+GELRL+VCIHH DN PSIINLLEVSN
Sbjct: 426 VTGTISPIVKIIYNPSKKYKSTSRRRTIEHASPSGELRLLVCIHHHDNTPSIINLLEVSN 485

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPI FYLLHLLQLTGRASPL I H   +   S R N    IINAFQIYQ++N++KV
Sbjct: 486 PTIKSPIYFYLLHLLQLTGRASPLFIDHQNGSHHDSQRFN---PIINAFQIYQKYNYDKV 545

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
           +MNAFTS+APY +MHDDVCMLALEKRV MVIVPFH+WRT+NGI ES N IRGVNKNILSK
Sbjct: 546 SMNAFTSIAPYVSMHDDVCMLALEKRVAMVIVPFHEWRTVNGIMESMNQIRGVNKNILSK 605

Query: 601 APCSVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALT 660
           APCSVGILIDR S  S T +S++NRVDLYKVGM+FVEG DDREALAYA RMAEHPMVALT
Sbjct: 606 APCSVGILIDRVSLQSTTMMSMMNRVDLYKVGMLFVEGADDREALAYATRMAEHPMVALT 665

Query: 661 VVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTM 720
           VV L+ P++  +    + VLDSEM+NEFKRIM T+ ++HCVY+EEM  DCVGLIN +R+M
Sbjct: 666 VVRLMLPESKTE-RRAELVLDSEMLNEFKRIMGTSGIQHCVYEEEMVKDCVGLINVIRSM 725

Query: 721 EHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGG 780
           E + D++LVGRRHDG+S LF+GLN+WNE+PELG IGDM  S+D +G   +LVVQQQTIG 
Sbjct: 726 EQNYDLMLVGRRHDGESELFLGLNEWNEYPELGIIGDMFASTDFSGAVAMLVVQQQTIGN 785

Query: 781 DQEFLEDFRCLMEESFSVDIKP 803
           D++ L+DFRCLMEESFSVDIKP
Sbjct: 786 DEDLLDDFRCLMEESFSVDIKP 803

BLAST of Lcy10g000340 vs. NCBI nr
Match: KAG7037023.1 (Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 603/802 (75.19%), Postives = 699/802 (87.16%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AA NGSWVCQ +  Y+SRG+F+GD PFS+GS++L+AQLSLSSLLT+LL CILTPLG
Sbjct: 1   MSTQAAQNGSWVCQPSSNYQSRGIFFGDTPFSYGSSILLAQLSLSSLLTTLLHCILTPLG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL+LGPSFYGE+  +L+ IFP+KSFYV+ETFAFFGC+VFMFLVGVKMDLT
Sbjct: 61  ESSFISQMLVGLMLGPSFYGEDSPVLEAIFPFKSFYVSETFAFFGCLVFMFLVGVKMDLT 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           +I ++G KAM IGV+AF+SP+ INF +  +LKN++DMDL LKNSL  IGA Q+SSSFHVI
Sbjct: 121 VIQRTGRKAMAIGVLAFISPLFINFILASYLKNSIDMDLQLKNSLTAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           AC L+D+ LLNSD+GRLALSSSMISG LSW ++VV FT++QTS  +QDALPWM LC++CM
Sbjct: 181 ACYLADINLLNSDIGRLALSSSMISGMLSWFAIVVAFTVQQTSNRQQDALPWMALCMVCM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+VIYI+RPIM WI+  TNNSGR +KE +V  +FLMLLFCALFSEFVGQHFMLGPMILG
Sbjct: 241 IILVIYILRPIMLWIIEQTNNSGRVIKEGYVLLVFLMLLFCALFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLG+ALVDKLDSFVS VMLP YFV+SG+RINLS+ NM S WIV +LA  S +G
Sbjct: 301 LAVPDGPPLGTALVDKLDSFVSYVMLPCYFVISGARINLSEINMRSAWIVQVLALGSFLG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIGT+LPSLYCKM LVDSLSLGLIMSTQGIADILI QHAMLLFMIDQKSY+MMVVAMMV
Sbjct: 361 KLIGTVLPSLYCKMPLVDSLSLGLIMSTQGIADILILQHAMLLFMIDQKSYAMMVVAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           VTGTISPIVK+IYNPSKKYK + RRRTIEHASP+GELRL+VCIHH DN PSIINLLEVSN
Sbjct: 421 VTGTISPIVKIIYNPSKKYKSTSRRRTIEHASPSGELRLLVCIHHHDNTPSIINLLEVSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PTIKSPI FYLLHLLQLTGRASPL I H   +   S R N    IINAFQIYQ++N++KV
Sbjct: 481 PTIKSPIYFYLLHLLQLTGRASPLFIDHQNGSHHDSQRFN---PIINAFQIYQKYNYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
           +MNAFTS+APY +MHDDVCMLALEKRV MVIVPFH+WRT+NGI ES N IRGVNKNILSK
Sbjct: 541 SMNAFTSIAPYVSMHDDVCMLALEKRVAMVIVPFHEWRTVNGIMESMNQIRGVNKNILSK 600

Query: 601 APCSVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALT 660
           APCSVGILIDR S  S T +S++NRVDLYKVGM+FVEG DDREALAYA RMAEHPMVALT
Sbjct: 601 APCSVGILIDRVSLQSTTMMSMMNRVDLYKVGMLFVEGADDREALAYATRMAEHPMVALT 660

Query: 661 VVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTM 720
           VV L+ P++  +    + VLDSEM+NEFKRIM T+ ++HCVY+EEM  DCVGLIN +R+M
Sbjct: 661 VVRLMLPESKTE-RRAELVLDSEMLNEFKRIMGTSGIQHCVYEEEMVKDCVGLINVIRSM 720

Query: 721 EHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGG 780
           E + D++LVGRRHDG+S LF+GLN+WNE+PELG IGDM  S+D +G   +LVVQQQTIG 
Sbjct: 721 EQNYDLMLVGRRHDGESELFLGLNEWNEYPELGIIGDMFASTDFSGAVAMLVVQQQTIGN 780

Query: 781 DQEFLEDFRCLMEESFSVDIKP 803
           D++ L+DFRCLMEESFSVDIKP
Sbjct: 781 DEDLLDDFRCLMEESFSVDIKP 798

BLAST of Lcy10g000340 vs. NCBI nr
Match: XP_022949065.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 599/802 (74.69%), Postives = 699/802 (87.16%), Query Frame = 0

Query: 1   MSTEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLG 60
           MST+AA NGSWVCQ +  Y+SRG+F+GD PFS+GS++L+AQLSLSSLLTSLLQCILTPLG
Sbjct: 1   MSTQAAQNGSWVCQPSSNYQSRGIFFGDTPFSYGSSILLAQLSLSSLLTSLLQCILTPLG 60

Query: 61  ESSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLT 120
           ESSFISQMLVGL+LGPSFYG +  +L+ IFP+KSFYV+ETFAFFGC+VFMFLVGVKMDLT
Sbjct: 61  ESSFISQMLVGLMLGPSFYGGDSPVLEAIFPFKSFYVSETFAFFGCLVFMFLVGVKMDLT 120

Query: 121 LIGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVI 180
           +I ++G KAMVIGV+AF+SP+ INF +  +LKN++DMDL LKNSL  IGA Q+SSSFHVI
Sbjct: 121 IIQRTGRKAMVIGVLAFISPLFINFILASYLKNSIDMDLQLKNSLTAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICM 240
           AC L+D+ LLNSD+GRLALSSSMISG LSW ++VV FT++QTS  +QDALPWM LC++CM
Sbjct: 181 ACYLADINLLNSDIGRLALSSSMISGMLSWFAIVVVFTVQQTSNRQQDALPWMALCIVCM 240

Query: 241 LIMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILG 300
           +I+VIYI+RPIM WI+  TNNSGR +KE +V  +FLMLLFCALFSEFVGQHFMLGPMILG
Sbjct: 241 IILVIYILRPIMLWIIEQTNNSGRVIKEGYVLLVFLMLLFCALFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIG 360
           LAVPDGPPLG+ALVDKLDSFVSSVMLP YFV+SG+RINLS+ NM S WIV +LA  S +G
Sbjct: 301 LAVPDGPPLGTALVDKLDSFVSSVMLPCYFVISGARINLSEINMRSAWIVQVLALGSFLG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 420
           KLIGT+LPSLYCKM LVDSLSLGLIMSTQGIADIL+ QHAMLLFMIDQKSY+MMVVAMMV
Sbjct: 361 KLIGTVLPSLYCKMPLVDSLSLGLIMSTQGIADILVLQHAMLLFMIDQKSYAMMVVAMMV 420

Query: 421 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 480
           VTG ISPIVK+IYNPSKKYK + RRRTIEHASP+GELRL+VCIHH DN PSIINLLEVSN
Sbjct: 421 VTGMISPIVKIIYNPSKKYKSTSRRRTIEHASPSGELRLLVCIHHHDNTPSIINLLEVSN 480

Query: 481 PTIKSPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKV 540
           PT+KSPI FYLLHLLQLTGRASPL I H   +   S R N    IINAFQIYQ++N++KV
Sbjct: 481 PTLKSPIYFYLLHLLQLTGRASPLFIDHQNGSHHDSQRFN---PIINAFQIYQKYNYDKV 540

Query: 541 TMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNAIRGVNKNILSK 600
           +MNAFTS+APY +MHDDVCMLALEKRV MVIVPFH+WR +NGI +S N IRGVNKNILSK
Sbjct: 541 SMNAFTSIAPYVSMHDDVCMLALEKRVAMVIVPFHEWRAVNGIMDSMNQIRGVNKNILSK 600

Query: 601 APCSVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALT 660
           APCSVGILIDR S  S   +S++NRVDLYKVGM+FVEG DDREALAYA RMAEHPMVALT
Sbjct: 601 APCSVGILIDRVSLQSTAMMSMMNRVDLYKVGMLFVEGADDREALAYATRMAEHPMVALT 660

Query: 661 VVHLIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTM 720
           VV L+ P++  +    + +LDSEM+NEFKRIMET+ ++HCVY+EEM  DCVGLIN +R+M
Sbjct: 661 VVRLMLPESKTE-RRAELILDSEMLNEFKRIMETSGIQHCVYEEEMVKDCVGLINVIRSM 720

Query: 721 EHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGG 780
           E + D++LVGRRHDG+S LF+GLN+WNE+PELG IGDM  S+D +G   +LVVQQQTIG 
Sbjct: 721 EQNYDLMLVGRRHDGESELFLGLNEWNEYPELGIIGDMFASTDFSGAVAMLVVQQQTIGN 780

Query: 781 DQEFLEDFRCLMEESFSVDIKP 803
           D++ L+DFRCLMEESF+VDIKP
Sbjct: 781 DEDLLDDFRCLMEESFNVDIKP 798

BLAST of Lcy10g000340 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 505.8 bits (1301), Expect = 6.7e-143
Identity = 304/809 (37.58%), Postives = 486/809 (60.07%), Query Frame = 0

Query: 2   STEAAHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGE 61
           S E + + S +C A     + GV+ GDNP  F   + + QL+L  ++T     IL P  +
Sbjct: 4   SEEPSTDASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQ 63

Query: 62  SSFISQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTL 121
              IS++L G+VLGPS  G +     TIFP +S  V ET A  G + F+FLVGV+MD+ +
Sbjct: 64  PRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMV 123

Query: 122 IGKSGTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIA 181
           + K+G +A+ I +   V P LI    +  +  + D  L     ++F+G   S ++F V+A
Sbjct: 124 VRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSED-HLGQGTYILFLGVALSVTAFPVLA 183

Query: 182 CLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICML 241
            +L++LKL+N+++GR+++S+++++   +W+ L +   + + S +   A  W+ +     +
Sbjct: 184 RILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAE-SDKTSFASLWVMISSAVFI 243

Query: 242 IMVIYIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGL 301
            + ++++RP + WI+  T   G    E H+  +   ++     ++ +G H + G  + GL
Sbjct: 244 AVCVFVVRPGIAWIIRKT-PEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGL 303

Query: 302 AVPDGPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWI-VHLLAFSSCIG 361
            +P+G PLG  L++KL+ FVS ++LP++F +SG + N++     + W+ + L+ F +C G
Sbjct: 304 VIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAG 363

Query: 362 KLIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMV 421
           K+IGT++ + +  M + + ++LGL+++T+G+ ++++        ++D ++++ MV+  +V
Sbjct: 364 KVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALV 423

Query: 422 VTGTISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSN 481
           +TG I+PIV ++Y P KK   S +RRTI+   P  ELR++VC+H   N P+IINLLE S+
Sbjct: 424 MTGVITPIVTILYKPVKK-SVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASH 483

Query: 482 PTIKSPICFYLLHLLQLTGRASPLLI-HHHRPTRRSSLRSN--LSDHIINAFQIYQQFNF 541
           PT +SPIC Y+LHL++LTGRAS +LI H+ R + R +L      SDHIINAF+ Y+Q + 
Sbjct: 484 PTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HA 543

Query: 542 NKVTMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTN-AIRGVNKN 601
             V +   T+++PY+TMH+DVC LA +KRV  +I+PFHK +T++G  ESTN A R VN+N
Sbjct: 544 AFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQN 603

Query: 602 ILSKAPCSVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPM 661
           +L  +PCSVGIL+DR  + +    S  N V L +V ++F  GPDDREALAYA RMA+HP 
Sbjct: 604 LLENSPCSVGILVDRGLNGATRLNS--NTVSL-QVAVLFFGGPDDREALAYAWRMAQHPG 663

Query: 662 VALTVVHLIDPKTNNDW-----------------HDPDQVLDSEMINEFKRIMETTEMKH 721
           + LTV+  I  +   D                  H   + LD + IN F+   E  E + 
Sbjct: 664 ITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFR--AENAEYES 723

Query: 722 CVYKEEMASDCVGLINALRTMEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDML 781
            VY E++ S+    + A+R+M+   D+ +VGR     S L  GL DW+E PELG IGD+L
Sbjct: 724 IVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLL 783

Query: 782 VSSDSTGTATVLVVQQQTIGGDQEFLEDF 789
            SSD   T +VLVVQQ      QE   DF
Sbjct: 784 ASSDFAATVSVLVVQQYVGSWAQEDDMDF 801

BLAST of Lcy10g000340 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 449.5 bits (1155), Expect = 5.7e-126
Identity = 257/770 (33.38%), Postives = 448/770 (58.18%), Query Frame = 0

Query: 12  VCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFISQMLVG 71
           VCQ NH   S+GVF G +P  +   +++ Q+S+  + + LL  +L PL +    +Q+L G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 72  LVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKSGTKAMV 131
           ++LGPS +G++ + ++   P       +T +  G  + +FL+G+++D ++I K+G+KA++
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 132 IGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLSDLKLLN 191
           IG  ++  P  +     LFLKNT ++   + + +  + +L + +SF V   +L++L +LN
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 192 SDVGRLALSSSMISGTLSW-VSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVIYIMRP 251
           SD+GRLA + S++    SW V+LV    +R  ++    A  W  + V  +++++ ++ RP
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFRMFLRDGTL----ASVWSFVWVTALILVIFFVCRP 266

Query: 252 IMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGPPLG 311
            + W+    + S     E   F + ++LL  +L SE +G H   G   LG+++PDGPPLG
Sbjct: 267 AIIWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLG 326

Query: 312 SALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIWIVHLLAFSSCIGKLIGTMLPSL 371
           + L  KL+ F +S+MLP +  +SG + N      + + I+  +   +   K +GT   S 
Sbjct: 327 TGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASA 386

Query: 372 YCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGTISPIVK 431
           YC + + D+ SL L+M  QG+ +I          +++ + ++++++ +++VTG    +V 
Sbjct: 387 YCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVV 446

Query: 432 LIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIKSPICFY 491
            +Y+PSK+Y+   +R  ++      + RL++C+++ +N PS++NLLE S P+  SPI  +
Sbjct: 447 CLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVF 506

Query: 492 LLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNAFTSVAP 551
            LHL++L GRA  +L+ HH+  +     +  S HI+N FQ ++Q N   +    FT+ AP
Sbjct: 507 TLHLVELKGRAHAVLVPHHQMNKLDP-NTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAP 566

Query: 552 YATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTN-AIRGVNKNILSKAPCSVGILI 611
           +++++DD+C LAL+K+  ++++PFHK   I+G  +  N +IR +N N+L KAPCSVGI I
Sbjct: 567 FSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFI 626

Query: 612 DRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVHLIDPKT 671
           DR  +    SV +        V ++F+EG DD EALA++ R+AEHP V++T++H     +
Sbjct: 627 DRGETEGRRSVLM--SYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSS 686

Query: 672 NNDWHDPD---QVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHDCDM 731
               H  D   ++ +S +IN+FK            Y+EE+  D V     + ++    D+
Sbjct: 687 LQQNHVVDVESELAESYLINDFKNF--AMSKPKISYREEIVRDGVETTQVISSLGDSFDL 746

Query: 732 ILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQ 777
           ++VGR HD +S++  GL DW+E PELG IGDM  SSD     +VLV+ QQ
Sbjct: 747 VVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDF--HFSVLVIHQQ 785

BLAST of Lcy10g000340 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 5.3e-116
Identity = 260/784 (33.16%), Postives = 437/784 (55.74%), Query Frame = 0

Query: 12  VCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFISQMLVG 71
           VCQA +   SRG+F   NP  +   +L+ Q+S+  + + L+  +L PL +    +Q+L G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 72  LVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKSGTKAMV 131
           +VLGPSF G N+  +    P     + +T +  G ++ +FL+G+K+D ++I K+G+KA++
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 132 IGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLSDLKLLN 191
           IG  ++  P  +     +F+  T+ +   + +      +L S +SF V   +L++L +LN
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNILN 208

Query: 192 SDVGRLALSSSMISGTLSW-VSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVIYIMRP 251
           S++GRLA   SM+    SW V+L      R  ++    AL      +I +L+++ ++ RP
Sbjct: 209 SELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTSLYALS----MIIGLLLVIYFVFRP 268

Query: 252 IMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGPPLG 311
           I+ W+      S         F + L+L   +L  E +G H   G   LG+++PDGPPLG
Sbjct: 269 IIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLPDGPPLG 328

Query: 312 SALVDKLDSFVSSVMLPVYFVVSGSRIN---LSKTNMTSIWIVHLLAFSSCIGKLIGTML 371
           + L  KL+ F S++ LP +  +SG + N   +++++   + ++ ++   +   K +GT  
Sbjct: 329 TELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGTAA 388

Query: 372 PSLYCKMSLVDSLSLGLIMSTQGIADI---LIFQHAMLLFMIDQKSYSMMVVAMMVVTGT 431
            S YC+  + D+L L  +M  QGI ++   ++++ A    ++D + ++++++ ++ VTG 
Sbjct: 389 ASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQ---VVDTECFNLVIITILFVTGI 448

Query: 432 ISPIVKLIYNPSKKYKRSMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIK 491
              +V  +Y+PSK+YK   +R  +       +LRL++ +++ +N PS++NLLE + PT  
Sbjct: 449 SRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRF 508

Query: 492 SPICFYLLHLLQLTGRASPLLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNA 551
           +PI F+ LHL++L GRA  LL  HH+  +     +  S HI+NAFQ ++Q     +    
Sbjct: 509 NPISFFTLHLVELKGRAHALLTPHHQMNKLDP-NTAQSTHIVNAFQRFEQKYQGALMAQH 568

Query: 552 FTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNA-IRGVNKNILSKAPC 611
           FT+ APY+++++D+C LAL+K+  ++++PFHK   I+G     N  IR +N N+L  APC
Sbjct: 569 FTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPC 628

Query: 612 SVGILIDRASSSSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVH 671
           SV I IDR  +    SV + N      V M+F+ G DD EALA   RMAE P + +T++H
Sbjct: 629 SVAIFIDRGETEGRRSVLMTN--TWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIH 688

Query: 672 LIDPKTNNDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHD 731
                   D  D   + +  +I++FK         H  Y EE+  D V     + ++   
Sbjct: 689 FRHKSALQD-EDYSDMSEYNLISDFKSYAANKGKIH--YVEEIVRDGVETTQVISSLGDA 748

Query: 732 CDMILVGRRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTIGGDQE 788
            DM+LVGR HD +S++  GL DW+E PELG IGDML S D     +VLVV QQ  G D  
Sbjct: 749 YDMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDF--HFSVLVVHQQQ-GDDLL 796

BLAST of Lcy10g000340 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 391.7 bits (1005), Expect = 1.4e-108
Identity = 252/798 (31.58%), Postives = 449/798 (56.27%), Query Frame = 0

Query: 6   AHNGSWVCQANHPYRSRGVFYGDNPFSFGSNVLIAQLSLSSLLTSLLQCILTPLGESSFI 65
           A N +  C A     S GVF GDNP  F   + I Q+ +  +LT +L  +L PL +   I
Sbjct: 2   ATNSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVI 61

Query: 66  SQMLVGLVLGPSFYGENISILKTIFPYKSFYVTETFAFFGCMVFMFLVGVKMDLTLIGKS 125
           ++++ G++LGPS  G + + L  +FP KS  V ET A  G + F+FL G+++D   + ++
Sbjct: 62  AEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRT 121

Query: 126 GTKAMVIGVMAFVSPMLINFFITLFLKNTVDMDLHLKNSLVFIGALQSSSSFHVIACLLS 185
           G KA+ I +     P  +    +  LK T+   ++    LVF+G   S ++F V+A +L+
Sbjct: 122 GKKALGIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILA 181

Query: 186 DLKLLNSDVGRLALSSSMISGTLSWVSLVVGFTMRQTSIEEQDALPWMTLCVICMLIMVI 245
           +LKLL +++GRLA+S++ ++   +W+ L +   +  ++     +L W+ L     +I   
Sbjct: 182 ELKLLTTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSL-WVFLSGCAFVIGAS 241

Query: 246 YIMRPIMFWIMTHTNNSGRAVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPD 305
           +I+ PI  WI +   + G  ++E+++ +   ++L C   ++ +G H M G  ++G+ +P 
Sbjct: 242 FIIPPIFRWI-SRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPK 301

Query: 306 GPPLGSALVDKLDSFVSSVMLPVYFVVSGSRINLSKTNMTSIW-IVHLLAFSSCIGKLIG 365
             P   ALV+K++  VS + LP+YFV SG + N++       W ++ L+  ++C GK++G
Sbjct: 302 EGPFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILG 361

Query: 366 TMLPSLYCKMSLVDSLSLGLIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGT 425
           T+  SL  K+ + ++++LG +M+T+G+ ++++        +++ +++++MV+  +  T  
Sbjct: 362 TLGVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFI 421

Query: 426 ISPIVKLIYNPSKKYKR--SMRRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPT 485
            +P+V  +Y P+++ K+    + R +E  +   +LR++ C H   + PS+INLLE S   
Sbjct: 422 TTPVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGI 481

Query: 486 IKSP-ICFYLLHLLQLTGRASP-LLIHHHRPT-----RRSSLRSNLSDHIINAFQIYQQF 545
            K   +C Y LHL +L+ R+S  L++H  R        R  + ++ +D ++ AFQ +QQ 
Sbjct: 482 EKGEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNAD-ADQVVVAFQAFQQ- 541

Query: 546 NFNKVTMNAFTSVAPYATMHDDVCMLALEKRVVMVIVPFHKWRTINGIEESTNA-IRGVN 605
             ++V +   T+++  + +H+D+C  A+ K+  +VI+PFHK + ++G  E+T    R VN
Sbjct: 542 -LSRVNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVN 601

Query: 606 KNILSKAPCSVGILIDRASSSSATSVSLVNRVDL-YKVGMVFVEGPDDREALAYAARMAE 665
           + +L +APCSVGI +DR    S    S V+  D+ Y V ++F  GPDDREALAY  RMAE
Sbjct: 602 RRVLLQAPCSVGIFVDRGLGGS----SQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAE 661

Query: 666 HPMVALTVV----------HLIDPKTNNDWHDPDQV----LDSEMINEFKRIMETTEMKH 725
           HP + LTV            +++ + +N+ ++   V     D E+++E ++I    E   
Sbjct: 662 HPGIVLTVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVK 721

Query: 726 CVYK--EEMASDCVGLINALRTMEHDCDMILVGRRHDGDSALFMGLNDWNEFPELGYIGD 776
            V K  E  A D    I  +R      ++ LVGR   G+ AL   + + +E PELG +G 
Sbjct: 722 FVEKQIENAAVDVRSAIEEVRR----SNLFLVGRMPGGEIAL--AIRENSECPELGPVGS 781

BLAST of Lcy10g000340 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 366.7 bits (940), Expect = 4.8e-101
Identity = 260/768 (33.85%), Postives = 424/768 (55.21%), Query Frame = 0

Query: 46  SLLTSLLQCILTPLGESSFISQMLVGLVLGPSFYGENISILKTIFPYKSF--YVTETFAF 105
           S L   L   L  LG   F S ML G++L  SF  EN S  +  F  + +   V    A 
Sbjct: 66  SFLWQFLHFFLRRLGMIRFTSHMLTGVLLSKSFLKEN-SAARRFFSTEDYKEIVFSLTAA 125

Query: 106 FGCMVFMFLVGVKMDLTLIGKSGTKAMVIGVMAFVSPML---INFFITLFLKNTVDMDLH 165
              M+F FL+GVKMD  LI  +G KA+ IG+ + +   L   + FF  L    T + D H
Sbjct: 126 CSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSD-H 185

Query: 166 LKNSL--VFIGALQSSSSFHVIACLLSDLKLLNSDVGRLALSSSMISGTLSWVSLVVGFT 225
             NSL  V I ++Q  SSF V+  LL +L+L NS++GRLA+SS++IS   + +   V   
Sbjct: 186 TLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIF 245

Query: 226 MRQTSIEEQDAL-------------PWMTLCVICMLIMV-IYIMRPIMFWIMTHTNNSGR 285
           M++   +EQ  L             P M   ++ + + + IY+ RP+MF+I+  T  SGR
Sbjct: 246 MKELK-DEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQT-PSGR 305

Query: 286 AVKESHVFSMFLMLLFCALFSEFVGQHFMLGPMILGLAVPDGPPLGSALVDKLDSFVSSV 345
            VK  ++ ++ +M+   A+ + +  Q   +GP ILGLAVP GPPLGSA++ K +S +   
Sbjct: 306 PVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGT 365

Query: 346 MLPVYFVVSGSRINLSKT-NMTSIWIVHLLAFSSCIGKLIGTMLPSLYCKMSLVDSLSLG 405
            LP +   S + I++S       +  + L+  +S + K I T +P+L+  M + D  +L 
Sbjct: 366 FLPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALS 425

Query: 406 LIMSTQGIADILIFQHAMLLFMIDQKSYSMMVVAMMVVTGTISPIVKLIYNPSKKYKRSM 465
           LIMS +GI ++  +  A     +  +++++  + + + +  I PI++ +Y+PS+ Y    
Sbjct: 426 LIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMY-AGY 485

Query: 466 RRRTIEHASPTGELRLMVCIHHQDNAPSIINLLEVSNPTIKSPICFYLLHLLQLTGRASP 525
            +R ++H  P  ELR++ CI+  D+   +INLLE   P+ +SP+  Y+LHL++L G+A+P
Sbjct: 486 EKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANP 545

Query: 526 LLIHHHRPTRRSSLRSNLSDHIINAFQIYQQFNFNKVTMNAFTSVAPYATMHDDVCMLAL 585
           + I H   TRR+   ++ S++++ +F+ +++  +  V ++ +T+++   TMH D+CMLAL
Sbjct: 546 IFISHKLQTRRTE-ETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLAL 605

Query: 586 EKRVVMVIVPFHKWRTING--IEESTNAIRGVNKNILSKAPCSVGILIDRA-------SS 645
                ++++PFH+  + +G  +  + N IR +NK++L  APCSVG+ + R+       SS
Sbjct: 606 NNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISS 665

Query: 646 SSATSVSLVNRVDLYKVGMVFVEGPDDREALAYAARMAEHPMVALTVVHLI----DPKTN 705
              T    V  +  Y + M+F+ G DDREA+  A RMA  P + +T+V LI      + N
Sbjct: 666 GRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKAREN 725

Query: 706 NDWHDPDQVLDSEMINEFKRIMETTEMKHCVYKEEMASDCVGLINALRTMEHDCDMILVG 765
             W   D++LD E++ + K    +  +    Y E+   D     + LR+M  D DM +VG
Sbjct: 726 TVW---DKMLDDELLRDVK----SNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVG 785

Query: 766 RRHDGDSALFMGLNDWNEFPELGYIGDMLVSSDSTGTATVLVVQQQTI 779
           R +   S    GL +W+EF ELG IGD+L S D    A+VLV+QQQ +
Sbjct: 786 RGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQL 820