Lcy10g000240 (gene) Sponge gourd (P93075) v1

BLAST of Lcy10g000240 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.5e-76
Identity = 193/539 (35.81%), Postives = 300/539 (55.66%), Query Frame = 0

Query: 3   WRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFS 62
           WR  +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK FG  S
Sbjct: 41  WR--KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSS 100

Query: 63  GLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTV 122
           G+A  Y PL +VL   +++G +GYGVQ+L ITN +  P Y L+FL  +LAG SICW NT 
Sbjct: 101 GIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTA 160

Query: 123 CYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLPLG 182
           C+++ IR+F +NR +A+ ++ S+ G+SA +Y+   ++I    +  ++  +LLLNS++PL 
Sbjct: 161 CFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLV 220

Query: 183 VC------VLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPL 242
           V       VL  P        +        F ++ V+ + T    +++S  + S +L+  
Sbjct: 221 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN-- 280

Query: 243 SGLVGILVLLLLPLVVAVIEKVKE------SVREGKEGRVYHFTVEEQKGDEDVERIGPK 302
              +G +VLL+ PL   ++   ++      + R   E   Y     ++  ++        
Sbjct: 281 --FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKT 340

Query: 303 NEAKLGRESEDEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRR 362
               +G   E   +   +E   R ++ R+ FWLY+  YF G T+GL + NNLGQIA+S  
Sbjct: 341 GYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG 400

Query: 363 SSSVSSLVSLSSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCP 422
            +S ++LV++ SSF FFGRL+ +  D    R +L   ++ GW  + +      FFLL+  
Sbjct: 401 QNS-TTLVTIYSSFSFFGRLLSAAPDFMHKRFRL---TRTGWFAIALLPTPIAFFLLAVS 460

Query: 423 TNMSLCVSTAT--IAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMA 482
           ++    + TAT  I + +G I + +VSIT++LFG N+  +NHN+++ NIP GS L+GY+A
Sbjct: 461 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIA 520

Query: 483 AFLYHKQA-----PATGK---CIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYSR 520
           A +Y   A     P       CIG +CY  TF  WGC    G + +L L+ RT+  Y R
Sbjct: 521 ASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

BLAST of Lcy10g000240 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 8.4e-227
Identity = 431/519 (83.04%), Postives = 457/519 (88.05%), Query Frame = 0

Query: 1   MPWRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGC 60
           MP  V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF C
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  FSGLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWIN 120
            SGLAANYLPLW VLIIGSSLGL+GYGVQYLFITNQ HSPSY LIF LTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLP 180
           TVCY+VAIRNF SNRQVAVGISTSYQGLSAKIYT+IV  IFSKRS KTAETFLL NSV P
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLV 240
           LGVCVL SP+ARI+  VEE G+LE+GFFVIFVITIATGVFAVVTSVGS+SR LSPLSGLV
Sbjct: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESE 300
           GILV LLLP+VV V+E VK+SVREGKE RVYH T+EE        RI P+NEAKL R+  
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------TRIRPENEAKLNRDGG 300

Query: 301 DEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSL 360
           D   EAMEEIGA EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAESR S SVSSLVSL
Sbjct: 301 D---EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSL 360

Query: 361 SSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTA 420
           SSSFGFFGRLMPSLLDHF SRRK +MKSKPGW+V LMGAMCGGFFLLS PT++ LCVSTA
Sbjct: 361 SSSFGFFGRLMPSLLDHFLSRRK-MMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTA 420

Query: 421 TIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAP-AT 480
            IAVCTGAITSISVS T+ELFGANNFSINHNVVVANIP GSFLFGYMAA+LY KQA    
Sbjct: 421 VIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA 480

Query: 481 GKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
           GKCIGVECYRTTF IWGC CSFGTLLAL+LFARTR+FYS
Sbjct: 481 GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYS 509

BLAST of Lcy10g000240 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.4e-226
Identity = 433/520 (83.27%), Postives = 459/520 (88.27%), Query Frame = 0

Query: 5   VLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGL 64
           V QWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF CFSGL
Sbjct: 7   VFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 66

Query: 65  AANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCY 124
           AANYLPLWLVL IGSSLGL+GYGVQYLFITNQ HSPSYWLIF LTVLAGNSICWINTVCY
Sbjct: 67  AANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCY 126

Query: 125 MVAIRNFA-SNRQVAVGISTSYQGLSAKIYTNIVDSIFS-KRSSKTAETFLLLNSVLPLG 184
           MVAI NFA SNRQVAVGISTSYQGLSAK+YT+IV SIFS K SSKTAETFLLLNSVLPLG
Sbjct: 127 MVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLG 186

Query: 185 VCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLVGI 244
           VCVL SPLAR +  VEE G LE+GFFVIFVITIATG FA +TSVGS+SR LS LSGLVGI
Sbjct: 187 VCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGLVGI 246

Query: 245 LVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERI-GPKNEAKLGRESED 304
           +V L+LPLVV V EKVKE V EGKEGRVYHFTVEE   + DVERI   +NE K+ R  E 
Sbjct: 247 MVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEE---NNDVERIMRGENERKVERTDEG 306

Query: 305 EAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSLS 364
              EAMEEIGA+EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR SSSVSSLVSLS
Sbjct: 307 ---EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 366

Query: 365 SSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTAT 424
           SSFGFFGRL+PS+LD+F SRR   MKSKPGWMV LMGA+CGGFFLL  P++ SLC+STA 
Sbjct: 367 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 426

Query: 425 IAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQA---PA 484
           IA+CTGAITSISVS TT+LFGA NFSINHNVVVANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 427 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 486

Query: 485 TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
            GKCIGVECYRTTF IWGCF SFGT LAL+LFARTR+FYS
Sbjct: 487 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYS 520

BLAST of Lcy10g000240 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 2.1e-222
Identity = 426/520 (81.92%), Postives = 454/520 (87.31%), Query Frame = 0

Query: 1   MPWRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGC 60
           MP  V QWLSL+GIIWLQSINGTNFNFPSYSS LK  LS+SQ QLNNLAFASDAGKLF C
Sbjct: 55  MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFAC 114

Query: 61  FSGLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWIN 120
            SGLAANYLPLW +LIIGS LGL+GYGVQYLFITNQ HSPSY LIF L+VLAGNSICWIN
Sbjct: 115 VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWIN 174

Query: 121 TVCYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLP 180
           TVCY+VAIRNF SNRQVAVGISTSYQGLSAKIYT+IV  IFSKRS KTAETFLL NSV P
Sbjct: 175 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 234

Query: 181 LGVCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLV 240
           LGVCVL  P+ARI+  VEE G+LE+GFFVIFVITIATGVFAVVTSVGS+SR LSPLSGLV
Sbjct: 235 LGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 294

Query: 241 GILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESE 300
           GILV LLLP+VV V+E VK+SVREGKE RVYH T+EE        RI P+NEAKL R+  
Sbjct: 295 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------TRIRPENEAKLNRDGG 354

Query: 301 DEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSL 360
           D   EAMEEIGA EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAES  S SVSSLVSL
Sbjct: 355 D---EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSL 414

Query: 361 SSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGG-FFLLSCPTNMSLCVST 420
           SSSFGFFGRLMPSLLDHF SRRK +MKSKPGW+V LMGAMCGG FFLLS PT++ LCVST
Sbjct: 415 SSSFGFFGRLMPSLLDHFLSRRK-MMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVST 474

Query: 421 ATIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAPA- 480
           A IAVCTGAITSISVS T+ELFGANNFSINHNVVVANIPFGSFLFGYMAA+LY KQA   
Sbjct: 475 AVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGD 534

Query: 481 TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
           TGKCIGVECYRTTF IWGC CS GTLLAL+LFARTR+FYS
Sbjct: 535 TGKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYS 564

BLAST of Lcy10g000240 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.9e-221
Identity = 419/519 (80.73%), Postives = 454/519 (87.48%), Query Frame = 0

Query: 5   VLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGL 64
           V QWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF CFSGL
Sbjct: 8   VFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 67

Query: 65  AANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCY 124
           AANYLPLWLVL IGSSLGL+GYGVQYLFITNQ HSPSYWLIF LTVLAGNSICWINTVCY
Sbjct: 68  AANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCY 127

Query: 125 MVAIRNF-ASNRQVAVGISTSYQGLSAKIYTNIVDSIFS-KRSSKTAETFLLLNSVLPLG 184
           MVAI NF +S+RQVAVGISTSYQGLSAK+YT+IV SIFS K SSKTAETFLLLNSVLPLG
Sbjct: 128 MVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLG 187

Query: 185 VCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLVGI 244
           VCVL SPLAR +  VEE G LE+GFFVIFVITIATG+FA +TSVGS+SR LS L GLVGI
Sbjct: 188 VCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGGLVGI 247

Query: 245 LVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESEDE 304
           +V L+LPLVV V+EKVKE V EGKEG+VYHFTVEE+    D ER+  +NE K+ R  +  
Sbjct: 248 MVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEK---NDEERMRGENERKVERTDDG- 307

Query: 305 AMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSLSS 364
             EAMEEIGA+EMVKRINFWLY GVY FGATLGLAFLNNLGQIAESR SSSVSSLVSLSS
Sbjct: 308 --EAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 367

Query: 365 SFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTATI 424
           SFGFFGRL+PS+LD+F SRR   MKSKPGWMV LMG +CGGFFLL  P++ SLC+STA I
Sbjct: 368 SFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAII 427

Query: 425 AVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQA---PAT 484
           A+CTGAITSISVS TT+LFGA NFSINHN+VVANIPFGSF+FGYMAAFLY KQA      
Sbjct: 428 AICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGVDP 487

Query: 485 GKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
           GKCIGVECYRTTF IWG F SFGT LAL+LFART++FYS
Sbjct: 488 GKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYS 520

BLAST of Lcy10g000240 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.8e-213
Identity = 417/520 (80.19%), Postives = 439/520 (84.42%), Query Frame = 0

Query: 5   VLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGL 64
           V QWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF CFSGL
Sbjct: 7   VFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 66

Query: 65  AANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCY 124
           AANYLPLWLVL IGSSLGL+GYGVQYLFITNQ HSPSYWLIF LTVLAGNSICWINTVCY
Sbjct: 67  AANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCY 126

Query: 125 MVAIRNFA-SNRQVAVGISTSYQGLSAKIYTNIVDSIFS-KRSSKTAETFLLLNSVLPLG 184
           MVAI NFA SNRQVAVGISTSYQGLSAK+YT+IV SIFS K SSKTAETFLLLNSVLPLG
Sbjct: 127 MVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLG 186

Query: 185 VCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLVGI 244
           VCVL SPLAR +  VEE G LE+GFFVIFVITIATG FA +TSVGS+SR LS LSGLVGI
Sbjct: 187 VCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGLVGI 246

Query: 245 LVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERI-GPKNEAKLGRESED 304
           +V L+LPLVV V EKVKE V EGKEGRVYHFTVEE   + DVERI   +NE K+ R  E 
Sbjct: 247 MVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEE---NNDVERIMRGENERKVERTDEG 306

Query: 305 EAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSLS 364
              EAMEEIGA+EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR SSSVSSLVSLS
Sbjct: 307 ---EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 366

Query: 365 SSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTAT 424
           SSFGFFGRL+PS+LD+F SRR   MKSKPGWMV LMGA+CG                   
Sbjct: 367 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG------------------- 426

Query: 425 IAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQA---PA 484
                GAITSISVS TT+LFGA NFSINHNVVVANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 427 -----GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 486

Query: 485 TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
            GKCIGVECYRTTF IWGCF SFGT LAL+LFARTR+FYS
Sbjct: 487 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYS 496

BLAST of Lcy10g000240 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 823.5 bits (2126), Expect = 1.0e-234
Identity = 438/516 (84.88%), Postives = 472/516 (91.47%), Query Frame = 0

Query: 5   VLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGL 64
           V QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF CFSGL
Sbjct: 6   VFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 65

Query: 65  AANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCY 124
           AANYLPLWLVL IG+SLGL+GYGVQYLF+TNQ+ SPSY +IFLLTVLAGNSICWINTVCY
Sbjct: 66  AANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCY 125

Query: 125 MVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLPLGVC 184
           MVAI NF +NRQ+AVGISTSYQGLSAKIYTNIV SIFSK SSKT ETFLLLNSVLP+GVC
Sbjct: 126 MVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVC 185

Query: 185 VLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLVGILV 244
           VLASPLARIL  VEE G LE+GFFVIFVITIATG+FA VTSVGS+SR LSPLSGLVGI+V
Sbjct: 186 VLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLVGIVV 245

Query: 245 LLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESEDEAM 304
            L+LPLVVAV+EKVKES++EGKE RVYHFTVEE K   DVER+  +N++KL R+ + E  
Sbjct: 246 FLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENK---DVERVRGENQSKLERDGDSE-- 305

Query: 305 EAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSLSSSF 364
           EAMEEIGA+EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR SSSVSSLVSLSSSF
Sbjct: 306 EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 365

Query: 365 GFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTATIAV 424
           GFFGRLMPS+LD+F SR+K  MKSKPGWMV LMG +CGGFFLL CPT++SLCVSTA IAV
Sbjct: 366 GFFGRLMPSILDYFLSRKK-CMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIAV 425

Query: 425 CTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQA--PATGKC 484
           CTGAITSISVS T+ELFGANNFSINHN++VANIPFGSFLFGYMAAFLYHKQA    TGKC
Sbjct: 426 CTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAGGDGTGKC 485

Query: 485 IGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
           IGVECYRTTFFIWGCF SFGTLLAL+LFART+TFYS
Sbjct: 486 IGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYS 515

BLAST of Lcy10g000240 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-226
Identity = 431/519 (83.04%), Postives = 457/519 (88.05%), Query Frame = 0

Query: 1   MPWRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGC 60
           MP  V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF C
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  FSGLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWIN 120
            SGLAANYLPLW VLIIGSSLGL+GYGVQYLFITNQ HSPSY LIF LTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLP 180
           TVCY+VAIRNF SNRQVAVGISTSYQGLSAKIYT+IV  IFSKRS KTAETFLL NSV P
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLV 240
           LGVCVL SP+ARI+  VEE G+LE+GFFVIFVITIATGVFAVVTSVGS+SR LSPLSGLV
Sbjct: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESE 300
           GILV LLLP+VV V+E VK+SVREGKE RVYH T+EE        RI P+NEAKL R+  
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------TRIRPENEAKLNRDGG 300

Query: 301 DEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSL 360
           D   EAMEEIGA EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAESR S SVSSLVSL
Sbjct: 301 D---EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSL 360

Query: 361 SSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTA 420
           SSSFGFFGRLMPSLLDHF SRRK +MKSKPGW+V LMGAMCGGFFLLS PT++ LCVSTA
Sbjct: 361 SSSFGFFGRLMPSLLDHFLSRRK-MMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTA 420

Query: 421 TIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAP-AT 480
            IAVCTGAITSISVS T+ELFGANNFSINHNVVVANIP GSFLFGYMAA+LY KQA    
Sbjct: 421 VIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA 480

Query: 481 GKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
           GKCIGVECYRTTF IWGC CSFGTLLAL+LFARTR+FYS
Sbjct: 481 GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYS 509

BLAST of Lcy10g000240 vs. NCBI nr
Match: XP_008457307.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 794.7 bits (2051), Expect = 5.0e-226
Identity = 433/520 (83.27%), Postives = 459/520 (88.27%), Query Frame = 0

Query: 5   VLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGL 64
           V QWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF CFSGL
Sbjct: 7   VFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 66

Query: 65  AANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCY 124
           AANYLPLWLVL IGSSLGL+GYGVQYLFITNQ HSPSYWLIF LTVLAGNSICWINTVCY
Sbjct: 67  AANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCY 126

Query: 125 MVAIRNFA-SNRQVAVGISTSYQGLSAKIYTNIVDSIFS-KRSSKTAETFLLLNSVLPLG 184
           MVAI NFA SNRQVAVGISTSYQGLSAK+YT+IV SIFS K SSKTAETFLLLNSVLPLG
Sbjct: 127 MVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLG 186

Query: 185 VCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLVGI 244
           VCVL SPLAR +  VEE G LE+GFFVIFVITIATG FA +TSVGS+SR LS LSGLVGI
Sbjct: 187 VCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGLVGI 246

Query: 245 LVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERI-GPKNEAKLGRESED 304
           +V L+LPLVV V EKVKE V EGKEGRVYHFTVEE   + DVERI   +NE K+ R  E 
Sbjct: 247 MVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEE---NNDVERIMRGENERKVERTDEG 306

Query: 305 EAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSLS 364
              EAMEEIGA+EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR SSSVSSLVSLS
Sbjct: 307 ---EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 366

Query: 365 SSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPTNMSLCVSTAT 424
           SSFGFFGRL+PS+LD+F SRR   MKSKPGWMV LMGA+CGGFFLL  P++ SLC+STA 
Sbjct: 367 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 426

Query: 425 IAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQA---PA 484
           IA+CTGAITSISVS TT+LFGA NFSINHNVVVANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 427 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 486

Query: 485 TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
            GKCIGVECYRTTF IWGCF SFGT LAL+LFARTR+FYS
Sbjct: 487 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYS 520

BLAST of Lcy10g000240 vs. NCBI nr
Match: KAG6583327.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019101.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 785.4 bits (2027), Expect = 3.0e-223
Identity = 427/520 (82.12%), Postives = 455/520 (87.50%), Query Frame = 0

Query: 1   MPWRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGC 60
           MP  V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF C
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  FSGLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWIN 120
            SGLAANYLPLW +LIIGS LGL+GYGVQYLFITNQ HSPSY LIF LTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLP 180
           TVCY+VAIRNF SNRQVAVGISTSYQGLSAKIYT+IV  IFSKRS KTAETFLL NSV P
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLV 240
           LGVCVL  P+ARI+  VEE G+LE+GFFVIFVITIATGVFAVVTSVGS+SR LSPLSGLV
Sbjct: 181 LGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESE 300
           GILV LLLP+VV V+E VK+SVREGKE RVYH T+EE        RI P+NEAKL R+  
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------TRIRPENEAKLNRDGG 300

Query: 301 DEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSL 360
           D   EAMEEIGA EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAESR S S SSLVSL
Sbjct: 301 D---EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESASSLVSL 360

Query: 361 SSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGG-FFLLSCPTNMSLCVST 420
           SSSFGFFGRLMPSLLDHF SRRK +MKSKPGW+V LMGAMCGG FFLLS PT++ LCVST
Sbjct: 361 SSSFGFFGRLMPSLLDHFLSRRK-MMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVST 420

Query: 421 ATIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAPA- 480
           A IAVCTGAITSISVS T+ELFGANNFSINHNVVVANIPFGSFLFGYMAA+LY KQA   
Sbjct: 421 AVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQARGD 480

Query: 481 TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
            GKCIGVECYRT+F IWGC CSFGTLLAL+LFARTR+FYS
Sbjct: 481 AGKCIGVECYRTSFMIWGCLCSFGTLLALLLFARTRSFYS 510

BLAST of Lcy10g000240 vs. NCBI nr
Match: XP_023519273.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.0 bits (2026), Expect = 4.0e-223
Identity = 428/520 (82.31%), Postives = 455/520 (87.50%), Query Frame = 0

Query: 1   MPWRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGC 60
           MP  V QWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF C
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  FSGLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWIN 120
            SGLAANYLPLW +LIIGS LGL+GYGVQYLFITNQ HSPSY LIF LTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLP 180
           TVCY+VAIRNF SNRQVAVGISTSYQGLSAKIYT+IV  IF+KRS KTAETFLL NSV P
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFTKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPLSGLV 240
           LGVCVL  P+ARI+  VEE G+L +GFFVIFVITIATGVFAVVTSVGS+SR LSPLSGLV
Sbjct: 181 LGVCVLVCPMARIVELVEEKGNLGVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKLGRESE 300
           GILV LLLP+VV V+E VK+SVREGKE RVYH T+EE        RI P+NEAKL R+  
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED------TRIRPENEAKLNRDGG 300

Query: 301 DEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSSSVSSLVSL 360
           D   +AMEEIGAREMV R+NFWLY GVYFFGATLGLAFLNNLGQIAESR S SVSSLVSL
Sbjct: 301 D---DAMEEIGAREMVTRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSL 360

Query: 361 SSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGG-FFLLSCPTNMSLCVST 420
           SSSFGFFGRLMPSLLDHF SRRK +MKSKPGWMV LMGAMCGG FFLLS PT++ LCVST
Sbjct: 361 SSSFGFFGRLMPSLLDHFLSRRK-MMKSKPGWMVGLMGAMCGGFFFLLSGPTDLILCVST 420

Query: 421 ATIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAP-A 480
           A IAVCTGAITSISVS T+ELFGANNFSINHNVVVANIPFGSFLFGYMAA+LY KQA   
Sbjct: 421 AVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGG 480

Query: 481 TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYS 519
            GKCIGVECYRTTF IWGC CSFGTLLAL+LFARTR+FYS
Sbjct: 481 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYS 510

BLAST of Lcy10g000240 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-116
Identity = 251/532 (47.18%), Postives = 336/532 (63.16%), Query Frame = 0

Query: 1   MPWRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGC 60
           M  ++L WLSLV I+WLQS NGTN +FP+YSSQLK+ L +SQ +LN L+FASDAGK+ G 
Sbjct: 1   MSRKILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGF 60

Query: 61  FSGLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWIN 120
            SG+AA YLPL LVL+ G SLG  GYG+QYL I  ++                  ICWIN
Sbjct: 61  ISGIAAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWIN 120

Query: 121 TVCYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLP 180
           T CY+VAI +F  NRQVAVGI+ SYQGLS KIYT++V S F     + A  +LLLNS++P
Sbjct: 121 TACYIVAINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVP 180

Query: 181 LGVCVLASPLARILIAVEEHG---------SLELGFFVIFVITIATGVFAVVTSVGSLSR 240
           L  C++ +P+      +  HG          +++GF V+FV+TIATG++AV TS+ S+  
Sbjct: 181 LVACLVTAPM------LMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPA 240

Query: 241 KLSPLSGLVGILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKN 300
            L     LVGI + LL PL + +    KE +   K  +  H    + +   D      ++
Sbjct: 241 VLV----LVGIALFLLAPLAIPIGVGFKELMSSRKTQQKVH----DLEAPVDKFYFVEED 300

Query: 301 EAKLGRESEDEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRS 360
             K   E E   +   EE+   ++ K+++FW+YFG+Y FG T+GL F NNLGQIAESR S
Sbjct: 301 HTKEEEEFEKAIIGVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGS 360

Query: 361 SSVSSLVSLSSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCPT 420
           ++ SSLV+LSSSFGFFGRL+PSLLD+FFSR K  M S P  M   + AM   F LL   +
Sbjct: 361 TATSSLVALSSSFGFFGRLLPSLLDYFFSRNK-YMPSSPVSMAGSLVAMVASFLLLLIDS 420

Query: 421 NMSLCVSTATIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAAFL 480
           +++L + TA I + +GA+TS+SV++T ELFG  +F +NHN+VV +IP GSF FG +AA +
Sbjct: 421 DIALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKI 480

Query: 481 YHKQAPA---TGKCIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYSRK 521
           Y   A      GKC G+ C++TT   WG  CS   LLA VL+ R R FYS+K
Sbjct: 481 YRDGAALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYSQK 499

BLAST of Lcy10g000240 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-77
Identity = 193/539 (35.81%), Postives = 300/539 (55.66%), Query Frame = 0

Query: 3   WRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFS 62
           WR  +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK FG  S
Sbjct: 41  WR--KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSS 100

Query: 63  GLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTV 122
           G+A  Y PL +VL   +++G +GYGVQ+L ITN +  P Y L+FL  +LAG SICW NT 
Sbjct: 101 GIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTA 160

Query: 123 CYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLPLG 182
           C+++ IR+F +NR +A+ ++ S+ G+SA +Y+   ++I    +  ++  +LLLNS++PL 
Sbjct: 161 CFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLV 220

Query: 183 VC------VLASPLARILIAVEEHGSLELGFFVIFVITIATGVFAVVTSVGSLSRKLSPL 242
           V       VL  P        +        F ++ V+ + T    +++S  + S +L+  
Sbjct: 221 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN-- 280

Query: 243 SGLVGILVLLLLPLVVAVIEKVKE------SVREGKEGRVYHFTVEEQKGDEDVERIGPK 302
              +G +VLL+ PL   ++   ++      + R   E   Y     ++  ++        
Sbjct: 281 --FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKT 340

Query: 303 NEAKLGRESEDEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRR 362
               +G   E   +   +E   R ++ R+ FWLY+  YF G T+GL + NNLGQIA+S  
Sbjct: 341 GYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG 400

Query: 363 SSSVSSLVSLSSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLLSCP 422
            +S ++LV++ SSF FFGRL+ +  D    R +L   ++ GW  + +      FFLL+  
Sbjct: 401 QNS-TTLVTIYSSFSFFGRLLSAAPDFMHKRFRL---TRTGWFAIALLPTPIAFFLLAVS 460

Query: 423 TNMSLCVSTAT--IAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMA 482
           ++    + TAT  I + +G I + +VSIT++LFG N+  +NHN+++ NIP GS L+GY+A
Sbjct: 461 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIA 520

Query: 483 AFLYHKQA-----PATGK---CIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYSR 520
           A +Y   A     P       CIG +CY  TF  WGC    G + +L L+ RT+  Y R
Sbjct: 521 ASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

BLAST of Lcy10g000240 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.9e-77
Identity = 200/538 (37.17%), Postives = 302/538 (56.13%), Query Frame = 0

Query: 3   WRVLQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFS 62
           WR  +W  LV  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK  G  S
Sbjct: 21  WR--RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSS 80

Query: 63  GLAANYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTV 122
           G A  Y P+  VL   +++GL+GYGVQ+L I + +  P Y L+ +   LAG SICW NTV
Sbjct: 81  GFAIAYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTV 140

Query: 123 CYMVAIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLPLG 182
            +++ IR+F +N  +A+ +  S+ G+SA +YT +     S +SS +++ +LLLNS++PL 
Sbjct: 141 SFILCIRHFKANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLI 200

Query: 183 VCVLA------SPLARILIAVEEHGSLELGFFVIF-VITIATGVFAVVTSVGSLSRKLSP 242
           V VLA      +P +        H   E   FV+F V+ + T  + ++ S G+     SP
Sbjct: 201 VSVLALWPVLTNPNSSETDTTRTHD--ETRIFVVFNVLALVTCFYLLLPSSGTYLAS-SP 260

Query: 243 LSGLVGILVLLLLPLVVAVIEKVKESVREGKEGRVYHFTVEEQKGDEDVERIGPKNEAKL 302
               +G + LLL PL V  ++ +  ++    E   +H +      + +  +I      K 
Sbjct: 261 RWHFLGAIFLLLFPLCVPFLDYIHRAL----ESCFHHHSSGYAAVNIEEPKILKIKSQKS 320

Query: 303 GRESEDEAMEAMEEIGAREMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAES--RRSSS 362
             E E + +   +E     +V+++ FWLY+  YF G T+GL + NNLGQIA+S  + SS+
Sbjct: 321 NAEEESDQVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSN 380

Query: 363 VSSLVSLSSSFGFFGRLMPSLLDHFFSRRKLLMKSKPGWMVVLMGAMCGGFFLL--SCPT 422
             SLV+L S+F F GRL+ S  D  F+R+KL   ++ GW  + +      FF+L  S  T
Sbjct: 381 AKSLVTLFSAFSFLGRLLSSAPD--FTRKKLKYLTRTGWFTISLLPTPLAFFILAYSPKT 440

Query: 423 NMS--LCVSTATIAVCTGAITSISVSITTELFGANNFSINHNVVVANIPFGSFLFGYMAA 482
           N +  L V+TA I + +G + + +VSIT+ELFG N+  +N N+++ NIP GS  +GYMA 
Sbjct: 441 NQTALLEVATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAG 500

Query: 483 FLYHKQAPATGK--------CIGVECYRTTFFIWGCFCSFGTLLALVLFARTRTFYSR 520
            +Y   A    K        C+G +CY  TF  WGC    G + +L LF RTR  Y R
Sbjct: 501 SVYDTNASLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVYHR 545

BLAST of Lcy10g000240 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 1.2e-76
Identity = 213/565 (37.70%), Postives = 310/565 (54.87%), Query Frame = 0

Query: 7   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGLAA 66
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+FG  SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 67  NYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCYMV 126
            Y PLW VL   + +G +GYGVQ+L ITN +  P Y L+FL  +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 127 AIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLPLGVCVL 186
            IRNF +NR +A+ ++ S+ G+SA +YT   ++I    +  + E +LLLN+++PL V   
Sbjct: 127 CIRNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPVSTELYLLLNALVPLFVSFA 186

Query: 187 ASPLARILIAVEEHGSLE-----------LGFFVIFVITIATGVFAVV----TSVGSLSR 246
           A      LI +     LE           L F ++ ++ +  GV+ ++    TS  + +R
Sbjct: 187 A------LIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSAR 246

Query: 247 KLSPLSGLVGILVLLLLPLVVA---VIEKVKESVREGKEGRVYHFT--VEEQKG------ 306
            L   S L+ IL L L  LV A    +  +  S R    G +      +E  KG      
Sbjct: 247 LLFGGSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREA 306

Query: 307 --------DEDVERIGPKNEAKLGRESED-----------EAMEAMEEIGAREMVKRINF 366
                   ++DV R     + K   E +D           +     EE     ++ R +F
Sbjct: 307 SLEGYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDF 366

Query: 367 WLYFGVYFFGATLGLAFLNNLGQIAES-RRSSSVSSLVSLSSSFGFFGRLMPSLLDHFFS 426
           WLY+  YF G T+GL + NNLGQIA+S  +SS  ++LV+L SSF FFGRL+ +  D+   
Sbjct: 367 WLYYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI-- 426

Query: 427 RRKLLMKSKPGWM-VVLMGAMCGGFFLLSCPTNMSLCVSTATIAVCTGAITSISVSITTE 486
            R  +  ++ GW+ V L+      F L S  +  +L   TA I + +G I + +VSIT+E
Sbjct: 427 -RAKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSE 486

Query: 487 LFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAPATGK-----CIGVECYRTTFFI 520
           LFG N+  +NHN+++ NIP GS ++G++AA +Y   + A  K     C+G +CY  TF  
Sbjct: 487 LFGPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMW 546

BLAST of Lcy10g000240 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.6e-73
Identity = 205/570 (35.96%), Postives = 308/570 (54.04%), Query Frame = 0

Query: 7   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFGCFSGLAA 66
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+FG  SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 67  NYLPLWLVLIIGSSLGLLGYGVQYLFITNQLHSPSYWLIFLLTVLAGNSICWINTVCYMV 126
            Y PLW VL   + +G +GYGVQ+L IT+ +  P Y ++FL  +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLP-YIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 127 AIRNFASNRQVAVGISTSYQGLSAKIYTNIVDSIFSKRSSKTAETFLLLNSVLPLGVCVL 186
            I NF +NR +A+ ++ S+ G+SA +YT   ++I    +  + E +LLLN+++PL V   
Sbjct: 127 CISNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPTSPELYLLLNALIPLIVSFT 186

Query: 187 ASPLARILIAVEEHGSLE-----------LGFFVIFVITIATGVFAVVTSVGSLSRKLSP 246
           A      +I +      E           L F ++ ++    GV+ ++   GS S  L+ 
Sbjct: 187 A------IIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLL--FGSNSSDLTS 246

Query: 247 LSGLV-GILVLLLLPLVV--AVIEK--------------------VKESVREGKEGRVYH 306
              L  G ++LL+ PL +   VI +                    V     E  +G + H
Sbjct: 247 ARLLFGGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAH 306

Query: 307 FTVEE--QKGDEDVERIGPKNEAKLGRESEDEA-------MEAMEEIGARE----MVKRI 366
               E  Q   +DV +  P     +  E  DE+        + +E +G       ++ R 
Sbjct: 307 EANREGYQLLSDDVVQ-NPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRS 366

Query: 367 NFWLYFGVYFFGATLGLAFLNNLGQIAES-RRSSSVSSLVSLSSSFGFFGRLMPSLLDHF 426
           +FWLY+  YF G T+GL + NNLGQIA+S  +SS+ ++LV+L S+F FFGRL+ +  D+ 
Sbjct: 367 DFWLYYITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI 426

Query: 427 FSRRKLLMKSKPGWM-VVLMGAMCGGFFLLSCPTNMSLCVSTATIAVCTGAITSISVSIT 486
              R  +  ++ GW+ + L+      F L S  T  +L   TA + + +G I + +VSIT
Sbjct: 427 ---RAKVYFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSIT 486

Query: 487 TELFGANNFSINHNVVVANIPFGSFLFGYMAAFLYHKQAPATGK--------CIGVECYR 520
           +ELFG N+  +NHN+++ NIP GS ++G++AA +Y        K        C+G +CY 
Sbjct: 487 SELFGPNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYY 546