Lcy09g019110 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g019110
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr09: 43119298 .. 43121241 (+)
RNA-Seq ExpressionLcy09g019110
SyntenyLcy09g019110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTCATCATACAAGTTCATTTCTTCTCTTACTACTAGCCATTGTCTTCTTCAACTCAACCTTTGCCGCTCAATTGACATTCAATGTTGTCGATTTCGGAGCCATACCCGATGCAAGAACCGACTCGTCCAAGGCCTTCCAATTAGCATGGGAACGTGCTTGTAGCTCTTCCAAAGCATCATCCATTTACGTGTCGAAGGGAAAATTCTATCTCCGCAGTGTAACTTTTAATGGTCCATGCAACAACAATGCCATCACCATACGCATTGATGGCACTCTCGTGGCTCCCTCAGACTTCCATGTTATTGGAAACTCTGAAAATTGGATCCTATTTCACAATGTCAATGGTGTTACTGTCTTAGGTGGCATTCTTGACGGTCAAGGAACTAGGTTGTGGACTTGCAAAAACTGTGGCAAGACTTGTCCTCGTGGAACCACGGTATACAAACTTTACTGTTTTTATGTATGTAAATACTTTGACATGTTTTATGACATATTATATTTATATATTAATTTATTTTTGAAAAGGTTAAGTTTGTATCAAATATCATGACTTTATTTTTGAAAAGGTTAAGTTTGTATCAAATATCATGACTGTTTTAATTGACTATATATATATATATTTATTTATTTTTATATATTATATATTAATTTATTTCTAACTTTTTTATCCTCTATTTTCCTTTTCAAACAATTTTGAAAGGTTTTGAAACATTTATTTATTATTTTATTCATATTAATAATTCAACTTAATTTATTAAGAGTCTTTTTTTTTTTTGAGAAGTTTATTAAGAGTCCTTAACTACAATAAATAAAGGATAAATTAAAATATAAAACACAAAATTAAATAAAAGACAAGTAAATTATCAAATACAAATTGTAGGATAATATTTTTAAAAGTTAAAATTATTACGTGTTCTCACACTTAGATATACTTATCAAATCACACTCTAATTTCAAAAGAACGGTTGTTTTGTGTATGTGCAGTCACTGGAATTTACCGATTCACAAAACATAATGATTAGTGGTTTTTTTTTTTTAAAAAAAGAATGATTAGTGGATTAACATCAATGAATAGCCAGATGTTTCATATAGTTGTGAATAAGTGCCGGAATGTAAAAATGCAAGGGATAAATGTATGGGCCTCCAATAATAGTCCAAACACCGATGACATTCATGTAGGTCTATCATCTTATGTCACCATCCTCAAGTCCAATATTGGCACCGGCGATGATTGCATCTTAATAGGTCCTGATAGTTCCAACTTGTGGATTGAGAATGTCACATGTGGACCTCGTCATGGAATAAGGTACGTACATCTTAAATTCTATGATCAATTATTTGAGTTCTTTTTTGGACTCCTATAATGAGAGAAAGATATGGCGTGTGACATTTGGGTTGATGGGGTGCAACATTGGTAGCTTAGGGAAAGATTTGAAGGAGGATGGAGTTCAGAATATCACGGTGAGATCTTCTAGATTTATCAACACTCAAAATGGGGTAAGAATAAAAAGTTGGGGAAGGCCAAGCAATGGGTTTGCAAGAAACATTCGTTTTCAACATCTCATAATGGCTAATGTTCAAAATCCTATCATCATCGATCAAAATTACTGCCCTCACAAATAAAGGTTGCTCTGGAAAGGTATGTATCAATATACATACAACTATACAACTATAGAGCATTTGATGTGAAAATGAATTATTACTTAATTTTGTTTGTTTTGTGAACTTGTGATTGAATAATGAATAGGTTTCTGGAATTAAAGTTAGCGACGTGAGGTATGAAGACATTCATGGAACATCTGCCACAGAGGTGGGCATGAAATTCAATTGTAGCCCAAAGTCTCCATGCACAGGAATAAGGCTGGAGGATATTAAGCTGAGCTACAAGAATCTAATAGCTAAAGCTTCATGTACTCATGTTAACCTACATAGAACTTAA

mRNA sequence

ATGACTCATCATACAAGTTCATTTCTTCTCTTACTACTAGCCATTGTCTTCTTCAACTCAACCTTTGCCGCTCAATTGACATTCAATGTTGTCGATTTCGGAGCCATACCCGATGCAAGAACCGACTCGTCCAAGGCCTTCCAATTAGCATGGGAACGTGCTTGTAGCTCTTCCAAAGCATCATCCATTTACGTGTCGAAGGGAAAATTCTATCTCCGCAGTGTAACTTTTAATGGTCCATGCAACAACAATGCCATCACCATACGCATTGATGGCACTCTCGTGGCTCCCTCAGACTTCCATGTTATTGGAAACTCTGAAAATTGGATCCTATTTCACAATGTCAATGGTGTTACTGTCTTAGGTGGCATTCTTGACGGTCAAGGAACTAGGTTGTGGACTTGCAAAAACTGTGGCAAGACTTGTCCTCGTGGAACCACGTGGTTTTTTTTTTTTAAAAAAAGAATGATTAGTGGATTAACATCAATGAATAGCCAGATGTTTCATATAGTTGTGAATAAGTGCCGGAATGTAAAAATGCAAGGGATAAATGTATGGGCCTCCAATAATAGTCCAAACACCGATGACATTCATGTAGGTCTATCATCTTATGTCACCATCCTCAAGTCCAATATTGGCACCGGCGATGATTGCATCTTAATAGGTCCTGATAGTTCCAACTTGTGGATTGAGAATGTCACATGTGGACCTCGTCATGGAATAAGCTTAGGGAAAGATTTGAAGGAGGATGGAGTTCAGAATATCACGGTGAGATCTTCTAGATTTATCAACACTCAAAATGGGGTTTCTGGAATTAAAGTTAGCGACGTGAGGTATGAAGACATTCATGGAACATCTGCCACAGAGGTGGGCATGAAATTCAATTGTAGCCCAAAGTCTCCATGCACAGGAATAAGGCTGGAGGATATTAAGCTGAGCTACAAGAATCTAATAGCTAAAGCTTCATGTACTCATGTTAACCTACATAGAACTTAA

Coding sequence (CDS)

ATGACTCATCATACAAGTTCATTTCTTCTCTTACTACTAGCCATTGTCTTCTTCAACTCAACCTTTGCCGCTCAATTGACATTCAATGTTGTCGATTTCGGAGCCATACCCGATGCAAGAACCGACTCGTCCAAGGCCTTCCAATTAGCATGGGAACGTGCTTGTAGCTCTTCCAAAGCATCATCCATTTACGTGTCGAAGGGAAAATTCTATCTCCGCAGTGTAACTTTTAATGGTCCATGCAACAACAATGCCATCACCATACGCATTGATGGCACTCTCGTGGCTCCCTCAGACTTCCATGTTATTGGAAACTCTGAAAATTGGATCCTATTTCACAATGTCAATGGTGTTACTGTCTTAGGTGGCATTCTTGACGGTCAAGGAACTAGGTTGTGGACTTGCAAAAACTGTGGCAAGACTTGTCCTCGTGGAACCACGTGGTTTTTTTTTTTTAAAAAAAGAATGATTAGTGGATTAACATCAATGAATAGCCAGATGTTTCATATAGTTGTGAATAAGTGCCGGAATGTAAAAATGCAAGGGATAAATGTATGGGCCTCCAATAATAGTCCAAACACCGATGACATTCATGTAGGTCTATCATCTTATGTCACCATCCTCAAGTCCAATATTGGCACCGGCGATGATTGCATCTTAATAGGTCCTGATAGTTCCAACTTGTGGATTGAGAATGTCACATGTGGACCTCGTCATGGAATAAGCTTAGGGAAAGATTTGAAGGAGGATGGAGTTCAGAATATCACGGTGAGATCTTCTAGATTTATCAACACTCAAAATGGGGTTTCTGGAATTAAAGTTAGCGACGTGAGGTATGAAGACATTCATGGAACATCTGCCACAGAGGTGGGCATGAAATTCAATTGTAGCCCAAAGTCTCCATGCACAGGAATAAGGCTGGAGGATATTAAGCTGAGCTACAAGAATCTAATAGCTAAAGCTTCATGTACTCATGTTAACCTACATAGAACTTAA

Protein sequence

MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLGGILDGQGTRLWTCKNCGKTCPRGTTWFFFFKKRMISGLTSMNSQMFHIVVNKCRNVKMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGISLGKDLKEDGVQNITVRSSRFINTQNGVSGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLEDIKLSYKNLIAKASCTHVNLHRT
Homology
BLAST of Lcy09g019110 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-105
Identity = 200/380 (52.63%), Postives = 244/380 (64.21%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M +  S F L L+ +   NS  A+ LT+NV   GA  D +TDS+KAF  AW +AC+S   
Sbjct: 1   MANRRSLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNP 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
             IYV  G F+LR V F+GPC NNAIT RI GTLVAPSD+ VIGN+ NWI FH+VNGVT+
Sbjct: 61  GVIYVPAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTI 120

Query: 121 LGGILDGQGTRLWTCKNC-GKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVNKCRN 180
            GGILDGQGT LW CK C G++CP G T   F      ++SGL S+NSQMFHIV+N  +N
Sbjct: 121 SGGILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQN 180

Query: 181 VKMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGP 240
           V+MQG+ V  S NSPNTD IHV +SS VTIL S I TGDDC+ IGP +SNLWIE V CGP
Sbjct: 181 VQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGP 240

Query: 241 RHGI---SLGKDLKEDGVQNITVRSSRFINTQNG-------------------------- 300
            HGI   SLGK+ +E GVQN+TV++  F  TQNG                          
Sbjct: 241 GHGISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVN 300

Query: 301 ---------------------VSGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRL 328
                                VSG+++SDV YEDIHGTSATEV +KF+CSPK PC  I+L
Sbjct: 301 VENPIVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKL 360

BLAST of Lcy09g019110 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.6e-76
Identity = 164/383 (42.82%), Postives = 220/383 (57.44%), Query Frame = 0

Query: 4   HTSSFLLLLLAIVFFNSTFAAQL------TFNVVDFGAIPDARTDSSKAFQLAWERACSS 63
           + +S L+L LA++  + T  + L      T NV+ +GA PD   DS+KAF  AW+ AC+S
Sbjct: 3   YNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACAS 62

Query: 64  SKASSIYVSKGKFYLRSVTFNG-PCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVN 123
           +  ++I V KG+F + ++ F+G  C    I+IRI G++VAP DF +I +S++WI F +V 
Sbjct: 63  ANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVT 122

Query: 124 GVTVLGGILDGQGTRLWTCK-NCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVN 183
            V++ GGILD QGT LW CK N G  CP G     F       ISGLTS+NSQ FHIV++
Sbjct: 123 DVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVID 182

Query: 184 KCRNVKMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENV 243
              NV + G+ V A  NSPNTD IHV  S  V I  S IGTGDDCI IGP S+N++I+ +
Sbjct: 183 NSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTI 242

Query: 244 TCGPRHGI---SLGKDLKEDGVQNITVRSSRFINTQNGV--------------------- 303
            CGP HGI   SLG+  +E GV N+TV +  F+ T NGV                     
Sbjct: 243 RCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHI 302

Query: 304 -------------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTG 328
                                    SG+KVS+VRYEDIHGTS TEV +  +CS + PCTG
Sbjct: 303 NMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTG 362

BLAST of Lcy09g019110 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.3e-52
Identity = 129/359 (35.93%), Postives = 177/359 (49.30%), Query Frame = 0

Query: 28  FNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYVSKGKFYLRSVTFNGPCNNNAIT 87
           FNV D+GA      D S+A   AW+ AC+S   S++ + KG + +  V   GPC  + I 
Sbjct: 10  FNVNDYGA--KGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIG 69

Query: 88  IRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLG-GILDGQGTRLWTCKNCGK--TCPR 147
            +IDG + AP+D      S+ W+ F+ ++G+TV G G LDGQG   W   NC K   C  
Sbjct: 70  FQIDGVVKAPADPSKF-KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKH 129

Query: 148 G--TTWFFFFKKRMISGLTSMNSQMFHIVVNKCRNVKMQGINVWASNNSPNTDDIHVGLS 207
                 F F K  M+  +TS+NS+MFHI V +C ++  Q + V A   S NTD IHVG+S
Sbjct: 130 AAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGIS 189

Query: 208 SYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGI---SLGKDLKEDGVQNITVRS 267
             VTI  + I TGDDCI IGP S N+ I  V CGP HGI   SLG+   E  V+ ITV+ 
Sbjct: 190 KGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKG 249

Query: 268 SRFINTQNGV-----------------------------------------------SGI 327
             F  T NGV                                               S I
Sbjct: 250 CTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRI 309

Query: 328 KVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLEDIKLSYKNLIAKASCTHVNLHRT 332
           K+S++ + +I GTS  +V +   CS   PC+ +++ +I LSY+     A+ T  N+  T
Sbjct: 310 KLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPT 365

BLAST of Lcy09g019110 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 4.5e-50
Identity = 128/369 (34.69%), Postives = 185/369 (50.14%), Query Frame = 0

Query: 17  FFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYVSKGKFY-LRSV 76
           F  ++     T +V DFGA  D +TD ++AF  AW++ACSS+ A ++ V KG  Y L+S+
Sbjct: 57  FVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSI 116

Query: 77  TFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLG---GILDGQGTRL 136
              GPC N+ +T++I GTL A        +   WI+F  VN ++V G   G++DG G   
Sbjct: 117 QLTGPC-NSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETW 176

Query: 137 W--TCK-NCGKTCPRGTTWFFFF--KKRMISGLTSMNSQMFHIVVNKCRNVKMQGINVWA 196
           W  +CK N  K C +  T   F+  K  ++  L   N+Q   I + KC NV++  + V A
Sbjct: 177 WQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTA 236

Query: 197 SNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGI---SLG 256
             +SPNTD IH+  +  + + +S IGTGDDCI I   S N+ I ++TCGP HGI   SLG
Sbjct: 237 PADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLG 296

Query: 257 KDLKEDGVQNITVRSSRFINTQNGV----------------------------------- 316
            D  +  V  +TV  ++   T NGV                                   
Sbjct: 297 DDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDY 356

Query: 317 ----------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLEDIKLSYKNLIA 329
                     S ++V +V Y DI GTSA+E  + FNCS   PC GI L+ + +       
Sbjct: 357 CDKSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKG----G 416

BLAST of Lcy09g019110 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-49
Identity = 117/357 (32.77%), Postives = 183/357 (51.26%), Query Frame = 0

Query: 27  TFNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYVSKGKFYLRSVTFNGPCNNNAI 86
           +F++   GA  + +TDS+KA Q AW  AC  +   +I + KG F +  + F GPC  + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-V 99

Query: 87  TIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLG-GILDGQGTRLWTCKNCGK--TCP 146
           TI++DG L+A +D     +  NWI    V+ + + G G LDGQG  +W+  +C K   C 
Sbjct: 100 TIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCK 159

Query: 147 --RGTTWFFFFKKRMISGLTSMNSQMFHIVVNKCRNVKMQGINVWASNNSPNTDDIHVGL 206
               +    F     +SG+T +NS+ FH+ + +C+++ ++ + V A  +SPNTD IH+G 
Sbjct: 160 ILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGD 219

Query: 207 SSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGISLG--------KDLKEDGVQ 266
           SS +TI  + IG GDDCI IGP +S + I  VTCGP HGIS+G        KD+ +  V+
Sbjct: 220 SSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 279

Query: 267 NITVRSSRF-------------------------------------------INTQNGVS 326
           + T++ + F                                           + T NG S
Sbjct: 280 DCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGAS 339

BLAST of Lcy09g019110 vs. ExPASy TrEMBL
Match: A0A6J1GNK7 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455565 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.5e-122
Identity = 235/378 (62.17%), Postives = 263/378 (69.58%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL LL I+FFNS FAA  TFNVV FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 24  MDPRTTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTS 83

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIYV  G+FYLR  TFNGPC N+AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 84  ASIYVPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 143

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  MISGLTS+NSQ+FHIVVN+C NV
Sbjct: 144 FGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNV 203

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHVGLSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 204 KMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 263

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF+NTQNGV                          
Sbjct: 264 HGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 323

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT   M F+CSPK PC GI+LE
Sbjct: 324 KNPIIIDQNYCPYNKDCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCVGIKLE 383

BLAST of Lcy09g019110 vs. ExPASy TrEMBL
Match: A0A6J1GMA1 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455564 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 3.0e-121
Identity = 234/378 (61.90%), Postives = 262/378 (69.31%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL LL I+FFNS FAA  TFNVV FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 1   MDPRTTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIYV  G+FYLR  TFNGPC N+AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 61  ASIYVPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  MISGLTS+NSQ+FHIVVN+C NV
Sbjct: 121 FGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHV LSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 181 KMEGLKVSASGNSPNTDGIHVELSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 240

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF+NTQNGV                          
Sbjct: 241 HGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT   M F+CSPK PC GI+LE
Sbjct: 301 KNPIIIDQNYCPYNKDCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCVGIKLE 360

BLAST of Lcy09g019110 vs. ExPASy TrEMBL
Match: A0A6J1I9T6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470567 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 2.5e-120
Identity = 232/378 (61.38%), Postives = 261/378 (69.05%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL L  ++FFNS FAA  T NVV+FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 1   MDPRTTSFLLSLQLVIFFNSPFAASETLNVVNFGAKPDAKTDSSKAFQAAWARACTSSTS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           SSIYV  G+FYLR  TFNGPC ++AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 61  SSIYVPNGRFYLRHATFNGPCRSDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  +ISGLTS+NSQ+FHIVVN+C NV
Sbjct: 121 FGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNILISGLTSLNSQIFHIVVNECHNV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHVGLSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 181 KMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 240

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF NTQNGV                          
Sbjct: 241 HGISIGSLGKALKEDGVQNVTVKSCRFTNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT   M F+CSPK PC GIRLE
Sbjct: 301 KNPIIIDQNYCPYNKNCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCVGIRLE 360

BLAST of Lcy09g019110 vs. ExPASy TrEMBL
Match: A0A6J1I881 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470566 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.8e-119
Identity = 230/378 (60.85%), Postives = 259/378 (68.52%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL L  ++FFNS FAA  T NVV+FGA PDA+TDSSKAFQ AW RAC+S  +
Sbjct: 1   MDPRTTSFLLSLQLVIFFNSPFAASETLNVVNFGAKPDAKTDSSKAFQAAWARACTSCTS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIYV  G+FYLR  TFNGPC ++AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 61  ASIYVPNGRFYLRHATFNGPCRSDAINIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCP G T   F   K  +ISGLTS+NSQ+FHIVVN+C NV
Sbjct: 121 FGGTLDGQGAGLWACKRSAKTCPLGATSLEFTDSKNILISGLTSLNSQIFHIVVNECHNV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHVGLSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 181 KMEGLKVTASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 240

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF NTQNGV                          
Sbjct: 241 HGISIGSLGKALKEDGVQNVTVKSCRFTNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT   M F+CSPK PC GIRLE
Sbjct: 301 KNPIIIDQNYCPYNKDCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCVGIRLE 360

BLAST of Lcy09g019110 vs. ExPASy TrEMBL
Match: A0A0A0K6Z5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G145950 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.7e-119
Identity = 229/380 (60.26%), Postives = 271/380 (71.32%), Query Frame = 0

Query: 3   HHTSSFL--LLLLAIVFFN--STFAAQLTFNVVDFGAIP-DARTDSSKAFQLAWERACSS 62
           H  +SFL  L L  ++ FN  + FA  LTFNV+DFG  P +A TDSSKA QLAW  ACSS
Sbjct: 6   HTIASFLFFLTLTVLMLFNNLAAFATPLTFNVLDFGTKPNNAETDSSKALQLAWMHACSS 65

Query: 63  SKASSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNG 122
           SK ++IYV K KFY+RS TFNGPC NN ITIRIDGTLVAPSDF +  NSENWILFHNVNG
Sbjct: 66  SKPATIYVPKAKFYVRSATFNGPCKNNDITIRIDGTLVAPSDFRLTSNSENWILFHNVNG 125

Query: 123 VTVLGGILDGQGTRLWTCKNCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVNKC 182
           VTV+GGI+DGQGT+LW CKN   TCP GTT   F   +  +++GLTS+NSQ+FHIVVN+C
Sbjct: 126 VTVIGGIIDGQGTQLWACKNSANTCPSGTTSLEFTNSQNILVNGLTSLNSQIFHIVVNQC 185

Query: 183 RNVKMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTC 242
           RNVKMQG+ V+A++NSPNTD IHVG SS+VTIL SNI TGDDCI IGP SSNLWIENV C
Sbjct: 186 RNVKMQGLKVFAASNSPNTDGIHVGDSSHVTILNSNIRTGDDCISIGPGSSNLWIENVVC 245

Query: 243 GPRHGI---SLGKDLKEDGVQNITVRSSRFINTQNGV----------------------- 302
           GP HGI   SLGK+LKE GVQN+TV+S +F++TQNGV                       
Sbjct: 246 GPGHGISIGSLGKELKEKGVQNVTVKSCKFMDTQNGVRIKSWGRQSSGFAKDIRFQHLTM 305

Query: 303 ------------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGI 326
                                   SG+ VSDV+Y++IHGTSAT V +KF+CSPK PC GI
Sbjct: 306 TNVQNPIIIDQNYCPHNQGCPGQSSGVTVSDVKYKNIHGTSATPVAIKFDCSPKFPCKGI 365

BLAST of Lcy09g019110 vs. NCBI nr
Match: XP_038888717.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 1.9e-122
Identity = 238/381 (62.47%), Postives = 273/381 (71.65%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVF--FN-STFAAQLTFNVVDFGAIPD-ARTDSSKAFQLAWERACS 60
           M  HT+SFLLLLL +V   FN + FAA LTFNVVDFGA PD A+TDSSKAF LAW RACS
Sbjct: 1   MAFHTNSFLLLLLLLVIMSFNLAAFAAPLTFNVVDFGAKPDNAKTDSSKAFLLAWTRACS 60

Query: 61  SSKASSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVN 120
           SS  +SIYV K KFY+RS TFNGPC NNAITIRIDGTLVA SDFH+   SE+WI+FHNVN
Sbjct: 61  SSIPTSIYVPKAKFYVRSATFNGPCKNNAITIRIDGTLVASSDFHLNVKSEHWIIFHNVN 120

Query: 121 GVTVLGGILDGQGTRLWTCKNCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVNK 180
           GV VLGG++DGQG+ LW CKN GK CPRG T   F   +  ++SGL S+NSQ+FHIVVN+
Sbjct: 121 GVNVLGGVIDGQGSGLWACKNSGKVCPRGATSLKFTDSQNILVSGLASLNSQIFHIVVNQ 180

Query: 181 CRNVKMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVT 240
           C NVKM G+ V AS+NSPNTD IH+G SSYVTI  S IGTGDDCI IGP +SNLWIEN+ 
Sbjct: 181 CHNVKMHGLKVSASSNSPNTDGIHIGGSSYVTISNSKIGTGDDCISIGPGTSNLWIENIA 240

Query: 241 CGPRHGI---SLGKDLKEDGVQNITVRSSRFINTQNGV---------------------- 300
           CGP HGI   SLGK+LKE+GV+N+TV+S  FINTQNGV                      
Sbjct: 241 CGPGHGISIGSLGKELKENGVENVTVKSCGFINTQNGVRIKSWGRPSSGFARNIIFQHIT 300

Query: 301 -------------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTG 326
                                    SGIK+SDV YEDIHGTSAT VG+KF+CSPK PC G
Sbjct: 301 MTNVQNPIIIDQNYCPHNQGCPGQDSGIKISDVTYEDIHGTSATPVGIKFDCSPKYPCKG 360

BLAST of Lcy09g019110 vs. NCBI nr
Match: XP_022953045.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 448.0 bits (1151), Expect = 7.2e-122
Identity = 235/378 (62.17%), Postives = 263/378 (69.58%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL LL I+FFNS FAA  TFNVV FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 24  MDPRTTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTS 83

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIYV  G+FYLR  TFNGPC N+AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 84  ASIYVPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 143

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  MISGLTS+NSQ+FHIVVN+C NV
Sbjct: 144 FGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNV 203

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHVGLSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 204 KMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 263

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF+NTQNGV                          
Sbjct: 264 HGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 323

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT   M F+CSPK PC GI+LE
Sbjct: 324 KNPIIIDQNYCPYNKDCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCVGIKLE 383

BLAST of Lcy09g019110 vs. NCBI nr
Match: XP_023511587.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 446.4 bits (1147), Expect = 2.1e-121
Identity = 233/378 (61.64%), Postives = 265/378 (70.11%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL LL I+FFNS FAA  TFNVV+FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 1   MDPRTTSFLLSLLLIIFFNSPFAASETFNVVNFGAKPDAKTDSSKAFQAAWARACTSSTS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIY+  G+FYLR  TFNGPC N+AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 61  ASIYIPNGRFYLRHATFNGPCINHAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  MISGLTS+NS +FHIVVN+C +V
Sbjct: 121 FGGTLDGQGAGLWACKRTAKTCPRGATSLEFTNSKNIMISGLTSLNSHIFHIVVNECHDV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHVGLSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 181 KMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 240

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF+NTQNGV                          
Sbjct: 241 HGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DIHGT+AT+  + F+CSPK PC GIRLE
Sbjct: 301 KNPIIIDQNYCPHNKDCPGQASGIKVSDVTYQDIHGTTATKFALTFDCSPKFPCVGIRLE 360

BLAST of Lcy09g019110 vs. NCBI nr
Match: XP_023511588.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 444.9 bits (1143), Expect = 6.1e-121
Identity = 234/378 (61.90%), Postives = 264/378 (69.84%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL LL I+FFNS FAA  TFNVV+FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 1   MDPRTTSFLLSLLLIIFFNSPFAASETFNVVNFGAKPDAKTDSSKAFQAAWARACTSSTS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIYV  G+FYLR  TFNGPC N+AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 61  ASIYVPNGRFYLRHATFNGPCINDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  MISGLTS+NS +FHIVVN+C +V
Sbjct: 121 FGGTLDGQGAGLWACKRTAKTCPRGATSLEFTNSKNIMISGLTSLNSHIFHIVVNECHDV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHVGLSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 181 KMEGLKVSASGNSPNTDGIHVGLSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 240

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF+NTQNGV                          
Sbjct: 241 HGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT+  M F+CSPK PC GIRLE
Sbjct: 301 KNPIIIDQNYCPHNKDCPGQASGIKVSDVTYQDIRGTTATKFAMTFDCSPKFPCVGIRLE 360

BLAST of Lcy09g019110 vs. NCBI nr
Match: XP_022953043.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 444.9 bits (1143), Expect = 6.1e-121
Identity = 234/378 (61.90%), Postives = 262/378 (69.31%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           M   T+SFLL LL I+FFNS FAA  TFNVV FGA PDA+TDSSKAFQ AW RAC+SS +
Sbjct: 1   MDPRTTSFLLSLLLIIFFNSPFAASETFNVVSFGAKPDAKTDSSKAFQAAWARACTSSTS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +SIYV  G+FYLR  TFNGPC N+AI I IDGTLVAPSDFHV  NS NWI+FH VNGV++
Sbjct: 61  ASIYVPNGRFYLRHATFNGPCRNDAIDIHIDGTLVAPSDFHVNANSPNWIIFHLVNGVSI 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
            GG LDGQG  LW CK   KTCPRG T   F   K  MISGLTS+NSQ+FHIVVN+C NV
Sbjct: 121 FGGTLDGQGAGLWACKRSAKTCPRGATSLEFTDSKNIMISGLTSLNSQIFHIVVNECHNV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
           KM+G+ V AS NSPNTD IHV LSS+VTIL S+I TGDDCI IGP +SNLWIENV CGP 
Sbjct: 181 KMEGLKVSASGNSPNTDGIHVELSSHVTILNSDISTGDDCISIGPGTSNLWIENVACGPG 240

Query: 241 HGI---SLGKDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HGI   SLGK LKEDGVQN+TV+S RF+NTQNGV                          
Sbjct: 241 HGISIGSLGKALKEDGVQNVTVKSCRFMNTQNGVRIKSWGRPSSGFARNIRFQHLKMTNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 327
                                SGIKVSDV Y+DI GT+AT   M F+CSPK PC GI+LE
Sbjct: 301 KNPIIIDQNYCPYNKDCPGQASGIKVSDVTYQDIRGTAATRFAMTFDCSPKFPCVGIKLE 360

BLAST of Lcy09g019110 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.4e-99
Identity = 186/368 (50.54%), Postives = 237/368 (64.40%), Query Frame = 0

Query: 10  LLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYVSKGK 69
           LL+L + FF S+ +AQ ++NV+ FGA PD +TD++KAF   W+ AC+SS+  +I V KG+
Sbjct: 4   LLVLFVFFFISSCSAQ-SYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGR 63

Query: 70  FYLRSVTFNG-PCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLGGILDGQ 129
           F LRSVTF+G  C    +T RIDGTLVAP+D+ VIGN + WI F +++G+TV GG+LD +
Sbjct: 64  FLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDAR 123

Query: 130 GTRLWTCKNCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVNKCRNVKMQGINVW 189
           G  LW CK  GK CP G T   F      ++SGLTS+NSQMFH+V+N C NVK+QG+ V 
Sbjct: 124 GASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVL 183

Query: 190 ASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGI---SL 249
           A+ NSPNTD IHV  SS V+I  + I TGDDC+ IGP ++ LWIENV CGP HGI   SL
Sbjct: 184 AAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 243

Query: 250 GKDLKEDGVQNITVRSSRFINTQNG----------------------------------- 309
           GKD  E GVQN+TV++  F  T NG                                   
Sbjct: 244 GKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQ 303

Query: 310 -----------VSGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLEDIKLSYKNL 326
                      VSGIK+SDV + DIHGTSATEVG+K +CS K PCTGIRLED+KL+Y+N 
Sbjct: 304 NYCPDHDCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNK 363

BLAST of Lcy09g019110 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 348.2 bits (892), Expect = 7.2e-96
Identity = 182/373 (48.79%), Postives = 236/373 (63.27%), Query Frame = 0

Query: 6   SSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYV 65
           SS L++LL ++  +S+ A   T+N++ +GA PD +TDS+KAF + W +AC+S K  +I V
Sbjct: 3   SSLLIVLLFLLSVSSSSAQ--TYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILV 62

Query: 66  SKGKFYLRSVTFNG-PCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLGGI 125
            KG+F LRS+ F+G  C   ++T RI GTLVAPSD+ VIG    WILF +++G++V GG+
Sbjct: 63  PKGRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGV 122

Query: 126 LDGQGTRLWTCKNCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVNKCRNVKMQG 185
           LD QG  LW+CK  GK CP G T   F   R  +ISGLTS+NSQMFH+ +N C NVK+ G
Sbjct: 123 LDAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDG 182

Query: 186 INVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGI- 245
           + V A  NSPNTD IHV  SS V+IL S I TGDDC+ IGP ++ LWIENV CGP HGI 
Sbjct: 183 VKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGIS 242

Query: 246 --SLGKDLKEDGVQNITVRSSRFINTQNG------------------------------- 305
             SLGK+  E GVQNITV+++ F  T+NG                               
Sbjct: 243 IGSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPI 302

Query: 306 ----------------VSGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLEDIKL 326
                           VSGIK+SDV + DIHGTSATEVG+K +CS K PCTGIR++D+KL
Sbjct: 303 VIDQNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKL 362

BLAST of Lcy09g019110 vs. TAIR 10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.2e-77
Identity = 164/383 (42.82%), Postives = 220/383 (57.44%), Query Frame = 0

Query: 4   HTSSFLLLLLAIVFFNSTFAAQL------TFNVVDFGAIPDARTDSSKAFQLAWERACSS 63
           + +S L+L LA++  + T  + L      T NV+ +GA PD   DS+KAF  AW+ AC+S
Sbjct: 3   YNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACAS 62

Query: 64  SKASSIYVSKGKFYLRSVTFNG-PCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVN 123
           +  ++I V KG+F + ++ F+G  C    I+IRI G++VAP DF +I +S++WI F +V 
Sbjct: 63  ANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVT 122

Query: 124 GVTVLGGILDGQGTRLWTCK-NCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVN 183
            V++ GGILD QGT LW CK N G  CP G     F       ISGLTS+NSQ FHIV++
Sbjct: 123 DVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVID 182

Query: 184 KCRNVKMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENV 243
              NV + G+ V A  NSPNTD IHV  S  V I  S IGTGDDCI IGP S+N++I+ +
Sbjct: 183 NSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTI 242

Query: 244 TCGPRHGI---SLGKDLKEDGVQNITVRSSRFINTQNGV--------------------- 303
            CGP HGI   SLG+  +E GV N+TV +  F+ T NGV                     
Sbjct: 243 RCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHI 302

Query: 304 -------------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTG 328
                                    SG+KVS+VRYEDIHGTS TEV +  +CS + PCTG
Sbjct: 303 NMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTG 362

BLAST of Lcy09g019110 vs. TAIR 10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 149/374 (39.84%), Postives = 215/374 (57.49%), Query Frame = 0

Query: 8   FLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKASSIYVSK 67
           F +++  I+     F A+ + NV+ FGA P+   +S+KAF  AW+ AC    +  IYV K
Sbjct: 11  FSIVITIIMSHFGQFDARTSLNVLSFGANPNGIVESAKAFSDAWDAACGVEDSVVIYVPK 70

Query: 68  GKFYLR-SVTFNG-PCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTVLGGIL 127
           G++ +   V F G  C +  IT+RIDGTL+ P D+ ++G  ENW  F  V+ VTVLGG  
Sbjct: 71  GRYLVSGEVRFEGESCKSREITLRIDGTLIGPQDYSLLGKKENWFSFSGVHNVTVLGGSF 130

Query: 128 DGQGTRLWTCKNCGKTCPRGTTWFFFFKKR--MISGLTSMNSQMFHIVVNKCRNVKMQGI 187
           D +G+ LW+CK  G  CP G T   F       I G+ S+NSQ+FHI +N+CRN+K++ +
Sbjct: 131 DAKGSTLWSCKANGYNCPEGATTLRFMDSNNVKIKGVLSLNSQLFHIAINRCRNIKIEDV 190

Query: 188 NVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPRHGISL 247
            + A + SPNTD IH+ LS+ + +  ++I TGDDCI IGP + NL ++ +TCGP HGIS+
Sbjct: 191 RIIAPDESPNTDGIHIQLSTDIEVRNASIKTGDDCISIGPGTKNLMVDGITCGPGHGISI 250

Query: 248 G---KDLKEDGVQNITVRSSRFINTQNGV------------------------------- 307
           G   K ++E GV+N+TV+++ F+ T NG+                               
Sbjct: 251 GSLAKSIEEQGVENVTVKNAVFVRTDNGLRIKSWPRHSNGFVERVRFLGAIMVNVSYPIL 310

Query: 308 ----------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLEDIKLS 328
                           SGIK++DV Y  I GTSATE+ +K +CS K PCTGIR++ I L+
Sbjct: 311 IDQNYCPGDSSCPSQESGIKINDVIYSGIMGTSATEIAIKMDCSEKVPCTGIRMQAINLT 370

BLAST of Lcy09g019110 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 152/366 (41.53%), Postives = 206/366 (56.28%), Query Frame = 0

Query: 1   MTHHTSSFLLLLLAIVFFNSTFAAQLTFNVVDFGAIPDARTDSSKAFQLAWERACSSSKA 60
           MT     F LL   + F + + +A   FNVV FGA PD  TDS+ AF  AW+ AC S+ +
Sbjct: 1   MTKSVIRFSLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASS 60

Query: 61  SSIYVSKGKFYLRSVTFNGPCNNNAITIRIDGTLVAPSDFHVIGNSENWILFHNVNGVTV 120
           +++ V KG F L+ +TF GPC +  IT ++ GT++AP D+   GNS  WILF+ VN  ++
Sbjct: 61  ATVVVPKGTFLLKVITFGGPCKSK-ITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSL 120

Query: 121 LGGILDGQGTRLWTCKNCGKTCPRGTTWFFF--FKKRMISGLTSMNSQMFHIVVNKCRNV 180
           +GG  D +    W+C+  G+ CP G     F   K  +ISG+ SMNSQ+ H+ +N C NV
Sbjct: 121 VGGTFDARANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNV 180

Query: 181 KMQGINVWASNNSPNTDDIHVGLSSYVTILKSNIGTGDDCILIGPDSSNLWIENVTCGPR 240
            ++ + + A  NSPNTD  HV  S+ VT   S + TGDDC+ IGP + NL I  + CGP 
Sbjct: 181 VVRNVKLVAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPG 240

Query: 241 HGISLG---KDLKEDGVQNITVRSSRFINTQNGV-------------------------- 300
           HG+S+G   K+LKEDGV+N+TV SS F  +QNGV                          
Sbjct: 241 HGVSIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNV 300

Query: 301 ---------------------SGIKVSDVRYEDIHGTSATEVGMKFNCSPKSPCTGIRLE 315
                                SG+K+S V Y++I GTSAT+  MK  CS  SPCTGI L+
Sbjct: 301 ENPIIIDQNYCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQ 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489792.2e-10552.63Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228181.6e-7642.82Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
Q6H9K01.3e-5235.93Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q8RY294.5e-5034.69Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
P262161.3e-4932.77Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GNK73.5e-12262.17polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455565 PE=3 SV=1[more]
A0A6J1GMA13.0e-12161.90polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111455564 PE=3 SV=1[more]
A0A6J1I9T62.5e-12061.38polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470567 PE=3 SV=1[more]
A0A6J1I8812.8e-11960.85polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470566 PE=3 SV=1[more]
A0A0A0K6Z54.7e-11960.26Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G145950 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888717.11.9e-12262.47polygalacturonase-like [Benincasa hispida][more]
XP_022953045.17.2e-12262.17polygalacturonase-like [Cucurbita moschata][more]
XP_023511587.12.1e-12161.64polygalacturonase-like [Cucurbita pepo subsp. pepo][more]
XP_023511588.16.1e-12161.90polygalacturonase-like [Cucurbita pepo subsp. pepo][more]
XP_022953043.16.1e-12161.90polygalacturonase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G43870.12.4e-9950.54Pectin lyase-like superfamily protein [more]
AT3G59850.17.2e-9648.79Pectin lyase-like superfamily protein [more]
AT2G43860.11.2e-7742.82Pectin lyase-like superfamily protein [more]
AT1G65570.11.7e-7639.84Pectin lyase-like superfamily protein [more]
AT1G05660.13.2e-7541.53Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 17..331
e-value: 1.2E-77
score: 263.7
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 270..327
e-value: 1.2E-8
score: 34.4
coord: 55..269
e-value: 1.1E-43
score: 149.6
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 6..269
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 6..269
coord: 269..325
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 269..325
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 11..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g019110.1Lcy09g019110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity