Lcy09g016350 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g016350
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	Chr09: 27859074 .. 27861769 (+)
RNA-Seq Expression	Lcy09g016350
Synteny	Lcy09g016350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCGCTGTCTGAACCATCGAATCTCTGCAAGCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGAACTCAACGAATCCTCCCTTCTGTTTCCCTCCCTTCCCCACCTCTTTCTTCCTTATCCCCATTTTTCACCATCGTCACAAATCAATGTACCGTCCTCTCTCTCTCTTTCTTGGAGGCTATACAGTATTTTTACGTTTCTTTTATAACTCTGTTCTTCAATTTCATTCTGTTTTAATTGCTATATAGCCTCAAGAAACAACCCTAACGCAGTTCCTGCAAGATGTTTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTGGCAGATTCTGCGGGCGACCTAGAACCGCCGGAAATTCATACGCAGGACACGGTGGAGCACCTTCTAACGCACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAAGCCGATTGCAGATGGAGCTGAATACCCCTGTAAAGGGCGGTGGTTTCCGTACCTCGATAGGTTTAGCTGTGGAAGCTCTTGGTTGAGTAGTGGTGAGATTTTGGAGGCACTGGATCCTCTCATATTGGACTCGAGGAAGCAGCGGTTCAAGAACGTGGTGCGGAACAGGAGCTACTCGGTTTGTTTGGTGGTGGAGGGGCTGAGTGATTTCGGCAACGTCTCGGCGGCATTCCGATCTGCCGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCCTGCGATGCTTCCAAAAGGTGCAACAACGATGGAGGTGAAATTGTTTTCGAATACATTTATTTTTTCTTTTAGGAAAATTGTTTGATTCTGGAATTCGGCAGCTGCTTTGTTTCCGTTGTGCTGGCGGGACTTCGTTACTGTTCATGGTGCTTTTACTTTGTTATTATTCAACGGGTCACTTGAATTCAATCAGGCGATTGCCATTGCGGGTTTTATATGGTTAGAAGGTTAAATCGATCACAATACATTTGATTTTCCATGGTAATTGTTAGATATGGGTGACTGAAATTAAGCACTGGAATGGAGTGTTTTCTTCCAGCAAATATGCATCCGTTGTATATGTATTTATATTTTTAACTCTTGTATTGGTTGTATGTATATTGTATATTATCATTGGATACTCTTTGCCAAATATTGATTATAATATATTCTTTTCCATTGCCCAAGGTGTCTCTTCATGTTGGTTTTCCATGTGAAATCTATGTGTTCATATGTTTTGTCTTCCTTTAGTTGCTGTCTTTATCTCGCCATATTTCATGACAGTAAGTGCCGTTTGTCTTGGACATGAAACTTTAAGAGGTGCCTGTATTCTCATGCTTATGCTGAATGAGATGATGCTTTGATGAATTAGGGTGCAACAGTGGTGATATATATTGTCTCTATGGATTATGGAAAGAGCTACTCCTATTTAATGGGTCATTCAACTTACTAAGAATGGCGGAATCTTCCATTTCTTACATCTTCTTTCACGAGTTTTTGTATTTTCTTCTGTCTTCTTCCAACATCATTGCAATTTCAATGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTTGGAACGAATGCGGTATCTATCAATTTTGCACTTTCACCCAATTCACAATGAAGTAACTTAACTTTGAGGTCATGTCTGCACTAGCTTTTTTAAGCTTATCTTGATAGCTGAGTTGTGTTTGTCCTGTTGTAGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGTAATTATCTTCTTGCTACAATAATATTGAGTCTGATCAAGTTTGGAGCCCTTTAAATTAAGGAATGACTGCAATAGTTGTTTTTTCTTTGTTATTTGATGCACCTTTTTGAATCACGATTCTCTCATGGTTTGATTAGCAAGTTTCTACTCATGAATGATGTCTGAATCAGGAGAAATTATACTTGTAGCTAGTTATGAACTTTGCAATTTATGTTAGTATCTGAGGTTCAAATTTCTTCTGTCTCAGGGGAATAAGCGATGAAGCCCTTGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGGACTTCTCGACTGGTAACTAAAAGAGTCATGTTTGATATTTGAATTCATTTATGCAATAAGCAGGGATTCTGTTGGGGTGTTACTAGGGGTATTCTGTGGAATTATGCCAGTATCTGGATAGCTCAATAAGAGAAAATCCTAGTTGTCTCTCGGAGTTGGCATGTGAGGATACTGAGTCGTGTGGGGTGGGGGCTGATACGAAGGATGTCAAATACTGTTTCTGTTATACGATCAACTATGTTCTTTAACGAACTTTTCAGGGCTATCATGGAGATCTCACATTAGATGAAAGGCAAATTCTACTTGCGGAGTTCTCTTTGCGGCATAGCAAGAATGCAATCAGTATCGCCACCGAGTACGCAAAGAGGAAGGGTACAGAGGAATTGGACTCGAATGGCAAGAATTTGGCCTCGAATGGCAAGAATTTGGCCTCTTGA

mRNA sequence

ACCGCTGTCTGAACCATCGAATCTCTGCAAGCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGAACTCAACGAATCCTCCCTTCTGTTTCCCTCCCTTCCCCACCTCTTTCTTCCTTATCCCCATTTTTCACCATCGTCACAAATCAATCCTCAAGAAACAACCCTAACGCAGTTCCTGCAAGATGTTTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTGGCAGATTCTGCGGGCGACCTAGAACCGCCGGAAATTCATACGCAGGACACGGTGGAGCACCTTCTAACGCACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAAGCCGATTGCAGATGGAGCTGAATACCCCTGTAAAGGGCGGTGGTTTCCGTACCTCGATAGGTTTAGCTGTGGAAGCTCTTGGTTGAGTAGTGGTGAGATTTTGGAGGCACTGGATCCTCTCATATTGGACTCGAGGAAGCAGCGGTTCAAGAACGTGGTGCGGAACAGGAGCTACTCGGTTTGTTTGGTGGTGGAGGGGCTGAGTGATTTCGGCAACGTCTCGGCGGCATTCCGATCTGCCGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCCTGCGATGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTTGGAACGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGCGATGAAGCCCTTGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGGACTTCTCGACTGGGCTATCATGGAGATCTCACATTAGATGAAAGGCAAATTCTACTTGCGGAGTTCTCTTTGCGGCATAGCAAGAATGCAATCAGTATCGCCACCGAGTACGCAAAGAGGAAGGGTACAGAGGAATTGGACTCGAATGGCAAGAATTTGGCCTCGAATGGCAAGAATTTGGCCTCTTGA

Coding sequence (CDS)

ATGGCGTCCTGCAGAATCGCAGCCCGTTCTTCTCTTATTCTGCTGGAAAGCTGCAGTCCCCGAAGAACTCAACGAATCCTCCCTTCTGTTTCCCTCCCTTCCCCACCTCTTTCTTCCTTATCCCCATTTTTCACCATCGTCACAAATCAATCCTCAAGAAACAACCCTAACGCAGTTCCTGCAAGATGTTTTGCTTCAATTTCGGCCGCAACATCTTCAATAGAAGTGGCAGATTCTGCGGGCGACCTAGAACCGCCGGAAATTCATACGCAGGACACGGTGGAGCACCTTCTAACGCACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAAGCCGATTGCAGATGGAGCTGAATACCCCTGTAAAGGGCGGTGGTTTCCGTACCTCGATAGGTTTAGCTGTGGAAGCTCTTGGTTGAGTAGTGGTGAGATTTTGGAGGCACTGGATCCTCTCATATTGGACTCGAGGAAGCAGCGGTTCAAGAACGTGGTGCGGAACAGGAGCTACTCGGTTTGTTTGGTGGTGGAGGGGCTGAGTGATTTCGGCAACGTCTCGGCGGCATTCCGATCTGCCGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCCTGCGATGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCCACCCCAGAATGTTTTAAAATTCTGAAATCTCGCGGTTATAGAATAGTCTCTACGCATCTTGGAACGAATGCGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGCGATGAAGCCCTTGAGCTATCAGATTTGCATTGCAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGACCGGACTTCTCGACTGGGCTATCATGGAGATCTCACATTAGATGAAAGGCAAATTCTACTTGCGGAGTTCTCTTTGCGGCATAGCAAGAATGCAATCAGTATCGCCACCGAGTACGCAAAGAGGAAGGGTACAGAGGAATTGGACTCGAATGGCAAGAATTTGGCCTCGAATGGCAAGAATTTGGCCTCTTGA

Protein sequence

MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVPARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPIADGAEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAKRKGTEELDSNGKNLASNGKNLAS

Homology

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BLAST of Lcy09g016350 vs. ExPASy Swiss-Prot
Match: O67577 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.7e-24
Identity = 60/188 (31.91%), Postives = 109/188 (57.98%), Query Frame = 0


Query: 150 LDPLILDSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 209
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAE-G 62

Query: 210 KRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPT 269
           K+ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 63  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 122

Query: 270 AIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGD 329
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  + G +  
Sbjct: 123 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEK-GMYSR 182

Query: 330 LTLDERQI 338
            +L E +I
Sbjct: 183 PSLSEEEI 188

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BLAST of Lcy09g016350 vs. ExPASy Swiss-Prot
Match: P0AGJ3 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.0e-22
Identity = 69/209 (33.01%), Postives = 107/209 (51.20%), Query Frame = 0


Query: 157 SRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 216
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 217 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNE 276
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 277 SRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGYHGDLTLD 336
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 337 ERQILLAEFSLRHSKNAISIATEYAKRKG 363
           E+Q LL E           +  + AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Lcy09g016350 vs. ExPASy Swiss-Prot
Match: P0AGJ2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.0e-22
Identity = 69/209 (33.01%), Postives = 107/209 (51.20%), Query Frame = 0


Query: 157 SRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 216
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 217 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNE 276
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 277 SRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGYHGDLTLD 336
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 337 ERQILLAEFSLRHSKNAISIATEYAKRKG 363
           E+Q LL E           +  + AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Lcy09g016350 vs. ExPASy Swiss-Prot
Match: P0AGJ4 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.0e-22
Identity = 69/209 (33.01%), Postives = 107/209 (51.20%), Query Frame = 0


Query: 157 SRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 216
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 217 HVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNE 276
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 277 SRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGYHGDLTLD 336
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 337 ERQILLAEFSLRHSKNAISIATEYAKRKG 363
           E+Q LL E           +  + AKRKG
Sbjct: 184 EQQRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of Lcy09g016350 vs. ExPASy Swiss-Prot
Match: Q72GI1 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 6.6e-21
Identity = 55/161 (34.16%), Postives = 92/161 (57.14%), Query Frame = 0


Query: 156 DSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 215
           ++R++R + V+R R   + +++E +    N+SA  R+ DA+G    H V  + +      
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAV--NPTGGVPTF 65

Query: 216 RHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGN 275
              S G+ KW+ + +     E F+ LK RG+ + +T L  +A    ++D++ PTA++ G 
Sbjct: 66  NETSGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGA 125

Query: 276 ESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 317
           E  G+S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 EKWGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of Lcy09g016350 vs. ExPASy TrEMBL
Match: A0A6J1F0V5 (uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC111441136 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.1e-179
Identity = 321/365 (87.95%), Postives = 337/365 (92.33%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIA R SL LL SCSPR TQRILPSVS  SPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 120
            RCF+S SAA SS+EV DS G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 180
            EYP +GRWFPYLD+FSC SSWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 KILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A EYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKGT 364
           KRK +
Sbjct: 361 KRKSS 365

[Close]

BLAST of Lcy09g016350 vs. ExPASy TrEMBL
Match: A0A6J1HYK4 (uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 5.2e-178
Identity = 318/365 (87.12%), Postives = 337/365 (92.33%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIA R SL LL SCS R TQ ILPSVS PSPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 120
            RCF+S SAA SS+EV D+ G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 180
           AEYP +GRWFPYLD+FSC +SWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 KILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC+IPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A EYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKGT 364
           KRK +
Sbjct: 361 KRKSS 365

[Close]

BLAST of Lcy09g016350 vs. ExPASy TrEMBL
Match: A0A0A0LC15 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.5e-172
Identity = 312/364 (85.71%), Postives = 332/364 (91.21%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRI +RSSL LL+SCSPRRTQRI  SV LPS  L S S FF   TN+ SR+NPNAV 
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFF---TNRFSRSNPNAVS 60

Query: 61  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 120
            RCFASISAATS +EV D   D+EPPE+ TQDTVEHLLTHS+DVARLMKMER+ +  DGA
Sbjct: 61  TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 120

Query: 121 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 180
           EYP KGRWFPYLD++SCGSSWL+SGEILEALD LILDSRK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK
Sbjct: 181 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240

Query: 241 ILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300
           ILKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGM
Sbjct: 241 ILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGM 300

Query: 301 VDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAK 360
           VDSFNVSVAAGILMHHAVC+RTSRLG HGDLT +ERQILLAEFSLRHSK+AISIA E AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAK 360

Query: 361 RKGT 364
           RKG+
Sbjct: 361 RKGS 361

[Close]

BLAST of Lcy09g016350 vs. ExPASy TrEMBL
Match: A0A5A7UJ80 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007650 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.6e-171
Identity = 312/364 (85.71%), Postives = 333/364 (91.48%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRI +RSSL LL+S SPRRTQR+ PSV LPS  L S SPFF   TN  SR+NPNAV 
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFF---TNTLSRSNPNAVS 60

Query: 61  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 120
           +RCFASISAATS +E+AD   D+EPPE+ TQDTVEHLLTHSDDV+RLMKMER+P+  DGA
Sbjct: 61  SRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGA 120

Query: 121 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 180
           EYP KGRWFPYLD++S GSSWL+SGEILEALD LILD+RK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECFK
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFK 240

Query: 241 ILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300
           ILKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGM
Sbjct: 241 ILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGM 300

Query: 301 VDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAK 360
           VDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AISIA E AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAK 360

Query: 361 RKGT 364
           RKG+
Sbjct: 361 RKGS 360

[Close]

BLAST of Lcy09g016350 vs. ExPASy TrEMBL
Match: A0A1S3B8M3 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.6e-171
Identity = 312/364 (85.71%), Postives = 333/364 (91.48%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRI +RSSL LL+S SPRRTQR+ PSV LPS  L S SPFF   TN  SR+NPNAV 
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFF---TNTLSRSNPNAVS 60

Query: 61  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 120
           +RCFASISAATS +E+AD   D+EPPE+ TQDTVEHLLTHSDDV+RLMKMER+P+  DGA
Sbjct: 61  SRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGA 120

Query: 121 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 180
           EYP KGRWFPYLD++S GSSWL+SGEILEALD LILD+RK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNST ECFK
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFK 240

Query: 241 ILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300
           ILKSRGYRIVSTHLG NAVSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGM
Sbjct: 241 ILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGM 300

Query: 301 VDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAK 360
           VDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AISIA E AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAK 360

Query: 361 RKGT 364
           RKG+
Sbjct: 361 RKGS 360

[Close]

BLAST of Lcy09g016350 vs. NCBI nr
Match: XP_038905162.1 (uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida])

HSP 1 Score: 660.6 bits (1703), Expect = 8.2e-186
Identity = 330/364 (90.66%), Postives = 345/364 (94.78%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIAARSSLILLESCS RR Q I PSVSLPS PLSS SPFFTIVTN+ SR+NPNAV 
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLSRSNPNAVS 60

Query: 61  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 120
            RCFAS SAATSSIEVAD+ GD EPPEIHTQDTVEHLLTHSDDVARLMKMER+P   DGA
Sbjct: 61  TRCFASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGA 120

Query: 121 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 180
           EYP +GRWFPYLD+FSCGSSWL+SGEILEALDPLILDSRK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF+
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFR 240

Query: 241 ILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300
           +LKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM
Sbjct: 241 VLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300

Query: 301 VDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAK 360
           VDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AIS+A E+AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFAK 360

Query: 361 RKGT 364
           RKG+
Sbjct: 361 RKGS 364

[Close]

BLAST of Lcy09g016350 vs. NCBI nr
Match: XP_038905163.1 (uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida])

HSP 1 Score: 641.3 bits (1653), Expect = 5.1e-180
Identity = 324/364 (89.01%), Postives = 338/364 (92.86%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIAARSSLILLESCS RR Q I PSVSLPS PLSS SPFFTIVTN+ S        
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLS-------- 60

Query: 61  ARCFASISAATSSIEVADSAGDLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADGA 120
            RCFAS SAATSSIEVAD+ GD EPPEIHTQDTVEHLLTHSDDVARLMKMER+P   DGA
Sbjct: 61  TRCFASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGA 120

Query: 121 EYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEG 180
           EYP +GRWFPYLD+FSCGSSWL+SGEILEALDPLILDSRK+RFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFK 240
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF+
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFR 240

Query: 241 ILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300
           +LKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM
Sbjct: 241 VLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300

Query: 301 VDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYAK 360
           VDSFNVSVAAGILMHHAVCDRTSRLG HGDLT +ERQILLAEFSLRHSK+AIS+A E+AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFAK 356

Query: 361 RKGT 364
           RKG+
Sbjct: 361 RKGS 356

[Close]

BLAST of Lcy09g016350 vs. NCBI nr
Match: XP_022933839.1 (uncharacterized protein LOC111441136 [Cucurbita moschata])

HSP 1 Score: 638.3 bits (1645), Expect = 4.3e-179
Identity = 321/365 (87.95%), Postives = 337/365 (92.33%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIA R SL LL SCSPR TQRILPSVS  SPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 120
            RCF+S SAA SS+EV DS G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 180
            EYP +GRWFPYLD+FSC SSWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 KILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A EYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKGT 364
           KRK +
Sbjct: 361 KRKSS 365

[Close]

BLAST of Lcy09g016350 vs. NCBI nr
Match: KAG6588498.1 (hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 635.2 bits (1637), Expect = 3.7e-178
Identity = 320/365 (87.67%), Postives = 336/365 (92.05%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIA R SL LL SCSPR TQRILPSVS  SPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 120
            RCF+S SAA SS+EV DS G DLEPPEIHTQDTVEHLLTHS DVARLMKMER+PI  DG
Sbjct: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120

Query: 121 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 180
            EYP +GRWFPYLD+FSC SSWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 KILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A EYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKGT 364
           KRK +
Sbjct: 361 KRKSS 365

[Close]

BLAST of Lcy09g016350 vs. NCBI nr
Match: XP_022969711.1 (uncharacterized protein LOC111468788 [Cucurbita maxima])

HSP 1 Score: 633.6 bits (1633), Expect = 1.1e-177
Identity = 318/365 (87.12%), Postives = 337/365 (92.33%), Query Frame = 0


Query: 1   MASCRIAARSSLILLESCSPRRTQRILPSVSLPSPPLSSLSPFFTIVTNQSSRNNPNAVP 60
           MASCRIA R SL LL SCS R TQ ILPSVS PSPPLSS SPFFT V+N SSRNNP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  ARCFASISAATSSIEVADSAG-DLEPPEIHTQDTVEHLLTHSDDVARLMKMERKPI-ADG 120
            RCF+S SAA SS+EV D+ G DLEPPEIHTQDTVEHLLTHSDDVARLMKMER+PI  DG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 AEYPCKGRWFPYLDRFSCGSSWLSSGEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVE 180
           AEYP +GRWFPYLD+FSC +SWL+SGE+L+ALDPLILDSRK+RFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 KILKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300
           +ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHC+IPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGYHGDLTLDERQILLAEFSLRHSKNAISIATEYA 360
           MVDSFNVSVAAGILMHHAVCDRTSRLG HGDLT  ERQILLAEFSLRH+KNAIS+A EYA
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

Query: 361 KRKGT 364
           KRK +
Sbjct: 361 KRKSS 365

[Close]

BLAST of Lcy09g016350 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 402.9 bits (1034), Expect = 2.9e-112
Identity = 210/332 (63.25%), Postives = 247/332 (74.40%), Query Frame = 0


Query: 31  SLPSPPLSSLSPFFTIVTNQSSRNNPNAVPARCFASISAATSSIEVADSAGDLEPPEIHT 90
           S P  PL   +P F+I        +P +V     + I  A  +   A +       E   
Sbjct: 14  SFPFVPLP--NPNFSITFISVRAFSPLSVLHPNSSCIVTARRTFHGAVALSPESLTEESP 73

Query: 91  QDTVEHLL-THSDDVARLMKMERKPIADGAEYPCKGRWFPYLDRFSCGSSWLSSGEILEA 150
           +DTV+ LL T+  + + LMKMER+      E  C+G WFPY DRF CG   LSS E+LEA
Sbjct: 74  KDTVKGLLTTNRGEASSLMKMERRCSLSNGEGDCRGSWFPYEDRFRCGEVHLSSREVLEA 133

Query: 151 LDPLILDSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 210
           + P +++ R  RF+ VV NRSYSVCLVVEGLSDFGN+SAAFRSADALG QSVHVVSCD+S
Sbjct: 134 VSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSS 193

Query: 211 KRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWSCPT 270
           KRY  NRHVSMGAEKWLDIE W++  ECFK+LKSRGYRI +THLG + VS+YDMDWSCPT
Sbjct: 194 KRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPT 253

Query: 271 AIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGYHGD 330
           AIVVGNE RGISDEALELSDL CSIPMNGMVDSFNVSVAAGILMHHAV DRT+RLG HGD
Sbjct: 254 AIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSVAAGILMHHAVSDRTTRLGSHGD 313

Query: 331 LTLDERQILLAEFSLRHSKNAISIATEYAKRK 362
           L+  E++IL+AEFSLRHS+++I IA E+AKRK
Sbjct: 314 LSEAEKEILMAEFSLRHSRSSICIAYEFAKRK 343

[Close]

BLAST of Lcy09g016350 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 56.2 bits (134), Expect = 6.6e-08
Identity = 46/200 (23.00%), Postives = 88/200 (44.00%), Query Frame = 0


Query: 144 GEILEALDPLILDSRKQRFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHV 203
           G +L+A  PL L   K+          YS+ + ++ ++D  N+ A  RSA   G   V V
Sbjct: 390 GLVLDA-SPLELVKVKELDPISSEEEKYSLWVALDEVTDPQNLGAIIRSAYFFGATGVVV 449

Query: 204 VSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDM 263
            + +++         S G+ + +++    +  +  +     G+R+V   +   AV++ ++
Sbjct: 450 CAKNSAPLSAVVSKASAGSLEVMELRYCKNMMQFLEASAENGWRVVGGSVSPKAVALNEV 509

Query: 264 DWSCPTAIVVGNESRG----ISDEALELSDLHCSIPMNG-----------------MVDS 323
               PT +V+GNE  G    +     +L  +  ++P                     V+S
Sbjct: 510 LPGSPTILVLGNEGTGLRPLVERSCTDLVRISGNMPNEVAVTESDDAEGEGFRSFLAVES 569

[Close]

BLAST of Lcy09g016350 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.1e-05
Identity = 42/153 (27.45%), Postives = 75/153 (49.02%), Query Frame = 0


Query: 175 LVVEGLSDFGNVSAAFRSADALGFQSVHVV--SCDA--SKRYRENRHVS----MGAEKWL 234
           LV++ + D GN+    RSA A  +    ++   CD    K  R +R  S    + +  W 
Sbjct: 199 LVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALRASRGASFQLPIVSGNWN 258

Query: 235 DIELWNSTPECFKILKSRGYRIVSTHLGTNAVSVYDMDWS-----CPTAIVVGNESRGIS 294
            ++L  +    F++    G+   +T      VS   ++++      P  +++G+E  G+S
Sbjct: 259 HLKLLENE---FQMKLLAGHPATTTQ-KLKPVSKLSVEFAQSLAEKPLCLILGSEGNGLS 318

Query: 295 DEALELSDLHCSIPMNGMVDSFNVSVAAGILMH 315
           ++A ++  L  SIPM G  +S NVSVA GI ++
Sbjct: 319 EQARKVCVL-VSIPMAGDFESLNVSVAGGIFLY 346

The following BLAST results are available for this feature:

BLAST of Lcy09g016350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67577	3.7e-24	31.91	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
P0AGJ3	6.0e-22	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
P0AGJ2	6.0e-22	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]
P0AGJ4	6.0e-22	33.01	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH ...	[more]
Q72GI1	6.6e-21	34.16	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]

BLAST of Lcy09g016350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0V5	2.1e-179	87.95	uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC1114411...	[more]
A0A6J1HYK4	5.2e-178	87.12	uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788...	[more]
A0A0A0LC15	1.5e-172	85.71	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824...	[more]
A0A5A7UJ80	3.6e-171	85.71	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B8M3	3.6e-171	85.71	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486...	[more]

BLAST of Lcy09g016350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905162.1	8.2e-186	90.66	uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida]	[more]
XP_038905163.1	5.1e-180	89.01	uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida]	[more]
XP_022933839.1	4.3e-179	87.95	uncharacterized protein LOC111441136 [Cucurbita moschata]	[more]
KAG6588498.1	3.7e-178	87.67	hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022969711.1	1.1e-177	87.12	uncharacterized protein LOC111468788 [Cucurbita maxima]	[more]

BLAST of Lcy09g016350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15390.1	2.9e-112	63.25	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	6.6e-08	23.00	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G38020.1	3.1e-05	27.45	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 149..356 e-value: 8.2E-59 score: 200.3
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 173..314 e-value: 1.8E-34 score: 118.9
None	No IPR available	PIRSR	PIRSR029256-1	PIRSR029256-1	coord: 220..316 e-value: 5.8E-5 score: 20.6
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 1..364
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 1..364
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	HAMAP	MF_02060	tRNA_methyltr_TrmH	coord: 155..349 score: 27.27566
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	CDD	cd18092	SpoU-like_TrmH	coord: 157..320 e-value: 4.78407E-80 score: 240.832
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 157..324

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g016350.1	Lcy09g016350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding