Lcy09g016300 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g016300
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	cucumisin-like
Location	Chr09: 27504184 .. 27510037 (+)
RNA-Seq Expression	Lcy09g016300
Synteny	Lcy09g016300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCGGCTAGCACTATTGACAGGAAGTTTGTGACTAATGTGAAGTTGGGTAATGGAGAAACTTTTCAGGTTAGTCCTAGATTGCTTGATTCATTCATGAATGTGTTCTGTTCTGAGCATTCGAGTCATTGATTAACATGCACACCACCTGTTTGTTAAGGGGATCTCTGTGAATACCTTCGATCTTGGAGGTAAGCTGTTACCACTCATATATGCTAGGTGATGCTCTTAATAGAACTGTAGGTTTTAAAAGAATGTTTACTTTATGGACTTGTTGGAAAAGTTGGGTGGCTGAAGGATGTTACTTTGTAAAGATCTTATGATGAGTCTAAATGTGCAGTGTTTTTCCTAAGTTGAAGAACAAGTTTTGGGTTTGGAGGAATTGAATTTTGGTGTCAAAATGCAGGTTCTGCTTCCCTAGTTCTTTGGATGTGAACCAAGTTCAAGCGAAGATTTTTACGATGAGATTGGTGATGGAGAAACAACTTTGAATAGTGGTGTAGTTGGTACCATAATGCAAGATGCTAATGACCAAGATGTCACCTTTATTTTCCCACGGCCTGCTTCCAAGTTAGACTTGAACACTGGAAAGAATGTTTCTGAGTACCTGAGATCAACCAGGTATAGTGACACATTGCTAAAAGGTTCATAGTATTTTAATACTATTTTAGTTTGTAAACTTGAGTTTTGTTTAGTCTCTAATGTTTCAAAGATTCTATTTTAGTTTTTGAACTTCATAAACTTTTTATAAAAGATATTTTTAGTTTTTTCCATTAATTTATATAATCCTTACCTCGAACAAAGTACCACAGACAAACCTTCCCTCACACAGACATCTATCCGTCCAGATCTCCTTTCATAGGGAGGAGTTTGCTCTTAGAGCCCGATTACGGATTCTATCCGTCCAGATCTCCTTTCATAGGGAGGGGTTTTCTCTTAGAGCCCGATTACGGATTTTATTCGTCCATATCTCCTTTCATAGGGAGGAGTTTGCTCTTAGAGCCAGATTACGGATTCTATCGATCCATATCTCCTTTCATAGGGAGGAGTTTACTCTTAGAGCCAGATTACAGATTCTGCATACGACTATTTCTTCAAGGCTAACAAATTCTTTGTCGACACTTGGGGATTCTACCAACATGGCTAAGTCAAGGTCTAGCTTTGATACCACTTGTTAAGACAGAAATCGTAATCAACGTAACAAGAATGAAGAGAGGGCGAAGAAAAAAAACAACACACAGATATACGTGGTTCACTAACGGTGTGTTAGCTACATCCACGGGCAGAGGGAGAGAACGTCTTATTATGAGAGGAGTATTCAGATTACAGAGATTAGGGCAAAAATAGGTTAAGAGGGTTTATATATGGCACCACTCTTAACCTTAACATAATAGGCCCAAAATAGTAAAGCCCAAAACAGAAAATAGGTTAAGAGGGTTTATATATGGCACCAGGCCCGTCGCCCTTGGACCCCGACTCGCTGACCGGTCAGCGAGACCCCCGTACTTGGTCGTTCGGAGCGCTTCATATCAGATTCAAGGCATATCAACAAATCTCCACCTTGACTTGAACTCTCTCACACATAACAGATGAACCAGATGTACCCAAAGAAAAGTACCAGATGCAACTCGAAAGTCACCAGATTCTCCCTCTGCTTCATAAAGTCCCATAGGGACCATTTAGCAATTCAGAACATTTAGCAAGTCCAATCAATGTTGGAACTTGCCACTCGGAACTGGCTTGGTGAACATATCAGCGGGGTTATGAGCTGTCCCAACTTTCATCACCTTAATCCTCTTCTCATTTCTCAGGAAGTGATATCTAACATCTATATGTTTAGTCCTCTCATGATGAACTTGATCCTTGGCTAGGCATATTGCACTCAAACTATCACAATATACCACAGCCTGATCATGATGCAGACCAAGGTCACCAACTAGTCCTTTCAACCATATTGCTTCTTTAGCAGCTTCTGTTAGTGCCATATACTCAACCTCAGTAGTAGACAAAGTAACTGTAGGCTGTAGGGTTGCCTTCCAACTAACAACAGAACCACCAAGGGTAAACACATAACCAGTCATGGACCTTCTACTGTCAACATCTCCAACATAGTCAGAATCAGAATAACCAGTCACTAAACATTCCGTGTCACTTCCATAAACGAGACCAATATCAGATGTACCTCTAAGGTAACGAAAAATCCTCTTCACAGCTTGCTAGTGTTCCTTTCTAGGTTGCCCCATGAACCTACTGACAACACTCACAGCATGTGCAAGATCAGGCCTAGTGCAGACCATATCATACATCAAACTTCCTACTGCACTAGCATATGGGACTCGAGACATGTATTCCTTCTCAGCTTCAGATTGTGATGCAAGTATAGAAGACAAACTAACACTTGCAACACTAGGAGTATCTATAGGCTTAGCTGAAGACATCCCAAACCTGAACAATATCTTCTGAATATATCCTTTCTGTGACAAGAAAAACTTCTTTTTATCTCTTTCCCTATAAATCTCCATACCCAAAATTTTCTGAGCAGCACCCAAATCTTTCATCTCAAATTCATCACTGAGAAGGTCTTTCAACTTCAGAATATCAGACTTGGACTTAGCAACTATCAACATGTCATCTACATACAGAACCAGATAAATCTTCAATCCAGTTTGATTTTCTACTAAGGTCTTCCACTGTTTGAAATTCTTGAAGGCTTCACTTTTGTGCTTCATCATAAATACCCAAGTCATTCTTGAGCAGTCATCAATCAGAGACAAAAAATATCTACTACCTCCAACAGATTCAACTTGAGAAGGACCCCAACAATCAGAATGGACATAATCAAGTGTTCCCTTCGTACTGTGAACACCCTTTGGAAACTTACTGCGATGAAGCTTGCCAAACACACAATGCTCACAAAATTCCAGATCCTGTACCTTGTGTCCACAAAGAAGATCTCTCTTTGACAAAATTTGAATCCCTCTTTCACTCATATGACCAAGTCTCATATGCCACAGCTTAGTCATATCTTCTTTGCGAACTAAAGAGGATGCAACAGCAACTAAACCAGTCAGTGTAGAACCTGCCAGATAATACAGAGTTCCACGTTTGACACCTTTCAGAACCACTTTAGAATCCTTGCAGACATAAGTAACTCCACCTTCACCTCTGAAACTGAAACCCTTACTGTCTAAAACACTCAAAGATATCAGACTCTTGGTCATTAGTGGAACATGCCTAACCTCATCCAAAGTGTAGAAAAGACCATCATGTGTCCGTATCCTGACTGAGCCTATCCCAACTACCTTACAGACAGCGTTGTTAGCCATAGTGACATTACCACCATCTACCTGCTGATAAGTTGAGAACCACTCCCTATTTGGACACATATGATACGACGCCCCAGAATCAAGAACCCACAGATCTTTAAGACATGGTTCTTCATTTACAGCTAGGGCTAAGTCATCTTCTGAATCAGTATTAGCTTCTGCCAAAGCGGCAGAACCTTCCTTCTTTTTATACCTATTATTCTTCTTTTTACAGTCATATTTCCAATGCCCCTTTTCTTTACAGTAATTACAGATGTCATTCGCTCTAGGACTAGTTTCATTAGGCTTCGAATTTTTACCATTAGACTTCTTTTTACCAGATTTCCTATGTCCTTTACTACTTGAAGCAACCAACCCTGACGCCTGATTATCTGTAACCGAACCTGCTACTTTATGACGAACTTCTCTAGTCAGAAGGGCTGTTTTTACTTCTTCTAGAGACAACTTATCTTTACCACTAATAAAAGAATCTACAAAATTCTCATACGACAACGGCAAAGATACTAACAGAATCAAAGCAGCATCCTTATCGTCTACCTTAACCTCAAGATTACGTAGATCTAACAAAATAGAGTTCAACTGGTCTATGTGATCTCTAAGGGGCGTACCTTCCTGCATCCGCAGACTGAACAATCGTTGCTTCAAGAGCAACTTCTTAGTCAAAGACTTCGTCATGTAGAGGCTCTCGAGCTTAGACCAAAGACCGGCAGCAGTATCCACACTCGCGACCTCGATGATGACGTCATCGGCCAGACATAACATAATCGTAGAATGGGCCTTCACCTCCAATGTTTTCCATTCTTCTTCATCAGCGTCCGCCTTCTTCTTACCAGACAGCGGCGCCCATAATCCCTGCTGCTTTAGCAGAGATCGCATCTTAATCTGCCATAGAGCAAAATTGTTCCTCCCGGTGAACTTGTCGATCTTCACGTTCATAGCCGACATCCTTGTACGCTGAAAAAACTGTTGCGGAATAACACGAACCGTAGCTCTGATACCACTTGTTAAGGGATGAGGACACGACACAGATCTCCACAATGGTATGATATTGTCCACTTTGGGCATGAGCCCTCATGACTTTACTTTGGGTTCCACCCAAAAGGCCTCATACCAATGGAGATACTTGTCCTCCCTTATAAACCCATGATCATCCCCTTGTCTAGCCAATGTGAGACTTTGGTTGCACTCCCAATTCCCAACACATATTTTATGTAAATCATGATGGCTAAGACAGAATATTTGAAAATTGAAAGACTAAAACCTAATATTTGAAAGTTTTAGAGTCCAAACCACATTGATTAACTAAAATAGAATTTAAACCTTACAAATTCTTTGGGTAGTTTTGACTTTCTTAGATTAAAATTGTTTGAAACCATCCTATTTTATTTCGTGTTTTACTTTGTTAGCAATTCGGAAGCTTTCATAAAATAAATTACTACCATTGGGGATCTGTTAGTTTCCATCTGTTGTTTCCATCTCATCAAGGGGACCTAACCTTTTTACACTGGACATTCTCAAGGTAACTCATCTGACTAAGTTTCCTCATGACTTGTTGTTAATGCCTTTTTGGATATTAACTTTTAGAAATCTCTTGTAGCCTATTTGGCAGCACAAGGAGTGGATATACTAACATCTTGGTCAGAAGCTTCAACAGCTACAAGTTTACCATGGGATAACAGAATAGTTCCATTTATTAACATAATCTCTGGCACATCCATGGCTTGTCCACATGCAACAGGAGTAGCTGCGTATGTCAAATCTTTCCACCGAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGGTAACAATTTGGCCTTAATTTCTTAGTACCAATAATTTAACTTAAATCATATGTTGGTTGCTTAAACTTTCATATTCATTTTGTTTGTCTATAGATTTTTAAATAATTTGTTTCGGTTGCCAATCCAAGTGTAGTTCAATTGGTTAAGGTATTCATATTTTGTTTCGGTTGCCAATCCAACTTTCATATTCATATTCATTTTGTTTGTCTATAGCTTAGTTCCGCATTTAGACTTTGACTAGTCGCGATTTTCTGTCTGTGTTTGTGATTGTTGAGCTATAGGATCTTGCACATGGTGATGATGGACTTTTTATTCGGATTATCTTTGTTGCATTTTTCATTTGCAAAGTCTCTAATATTTACTGTGTTGAGGTTGTGGTGTATGAGCGGAAGTTTACCCAACGGGAGACTTGCTTCAAGAATTTGGGATTGAGTCCTAAGCCTATTCACGATTGCTTTTCTTCTAGGCTTGGAAAAAAGGTGAGTTACTTAAACTAACTCAAGTAACGAACTTAGTTGTTTGCTCAAGTCCGATCCGTTTTTGTGAAAAATATAATATTCTTATGCTTTGACCGTTAAAGGGCAACTGGATAAACTAGCTAAATCTATGAGGATTGATTGTGTTTATGATCAGAACTATCATTTCTATGCAGCTTGAACTACAATATGCAGCTGAAACCAACAACGTTCGACCTCCTCATAAATACGTTCCTTGA

mRNA sequence

TTGCGGCTAGCACTATTGACAGGAAGTTTGTGACTAATGTGAAGTTGGGTAATGGAGAAACTTTTCAGTTCTTTGGATGTGAACCAAGTTCAAGCGAAGATTTTTACGATGAGATTGGTGATGGAGAAACAACTTTGAATAGTGGTGTAGTTGGTACCATAATGCAAGATGCTAATGACCAAGATGTCACCTTTATTTTCCCACGGCCTGCTTCCAAGTTAGACTTGAACACTGGAAAGAATGTTTCTGAGTACCTGAGATCAACCAGGTATAGTGACACATTGCTAAAAGCCTATTTGGCAGCACAAGGAGTGGATATACTAACATCTTGGTCAGAAGCTTCAACAGCTACAAGTTTACCATGGGATAACAGAATAGTTCCATTTATTAACATAATCTCTGGCACATCCATGGCTTGTCCACATGCAACAGGAGTAGCTGCGTATGTCAAATCTTTCCACCGAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGTTCAATTGGTTAAGGATCTTGCACATGGTGATGATGGACTTTTTATTCGGATTATCTTTGTTGCATTTTTCATTTGCAAAGTCTCTAATATTTACTGTGTTGAGGTTGTGGTGTATGAGCGGAAGTTTACCCAACGGGAGACTTGCTTCAAGAATTTGGGATTGAGTCCTAAGCCTATTCACGATTGCTTTTCTTCTAGGCTTGGAAAAAAGCTTGAACTACAATATGCAGCTGAAACCAACAACGTTCGACCTCCTCATAAATACGTTCCTTGA

Coding sequence (CDS)

TTGCGGCTAGCACTATTGACAGGAAGTTTGTGACTAATGTGAAGTTGGGTAATGGAGAAACTTTTCAGTTCTTTGGATGTGAACCAAGTTCAAGCGAAGATTTTTACGATGAGATTGGTGATGGAGAAACAACTTTGAATAGTGGTGTAGTTGGTACCATAATGCAAGATGCTAATGACCAAGATGTCACCTTTATTTTCCCACGGCCTGCTTCCAAGTTAGACTTGAACACTGGAAAGAATGTTTCTGAGTACCTGAGATCAACCAGGTATAGTGACACATTGCTAAAAGCCTATTTGGCAGCACAAGGAGTGGATATACTAACATCTTGGTCAGAAGCTTCAACAGCTACAAGTTTACCATGGGATAACAGAATAGTTCCATTTATTAACATAATCTCTGGCACATCCATGGCTTGTCCACATGCAACAGGAGTAGCTGCGTATGTCAAATCTTTCCACCGAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGTTCAATTGGTTAAGGATCTTGCACATGGTGATGATGGACTTTTTATTCGGATTATCTTTGTTGCATTTTTCATTTGCAAAGTCTCTAATATTTACTGTGTTGAGGTTGTGGTGTATGAGCGGAAGTTTACCCAACGGGAGACTTGCTTCAAGAATTTGGGATTGAGTCCTAAGCCTATTCACGATTGCTTTTCTTCTAGGCTTGGAAAAAAGCTTGAACTACAATATGCAGCTGAAACCAACAACGTTCGACCTCCTCATAAATACGTTCCTTGA

Protein sequence

AASTIDRKFVTNVKLGNGETFQFFGCEPSSSEDFYDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGKNVSEYLRSTRYSDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTSMACPHATGVAAYVKSFHRTWSPAAIKSALMTTVQLVKDLAHGDDGLFIRIIFVAFFICKVSNIYCVEVVVYERKFTQRETCFKNLGLSPKPIHDCFSSRLGKKLELQYAAETNNVRPPHKYVP

Homology

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BLAST of Lcy09g016300 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.6e-18
Identity = 76/249 (30.52%), Postives = 109/249 (43.78%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQFFGCE-----------------PSSSED-------FYD 60
           AAS IDR F + + LGNG++F   G                     +++D       F D
Sbjct: 332 AASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTDDKYLARYCFSD 391

Query: 61  EI----------------GDGETTLNS-GVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
            +                G  E+T+ S G  G I+      D   IF  PA+ ++ + G 
Sbjct: 392 SLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGD 451

Query: 121 NVSEYLRSTRYSDT-----------------------------LLKAYLAAQGVDILTSW 180
            +  Y+ STR +                               LLK  +AA G+DIL ++
Sbjct: 452 IIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILAAF 511

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BLAST of Lcy09g016300 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.7e-18
Identity = 73/238 (30.67%), Postives = 103/238 (43.28%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQFFGCEPSSSEDFYDEIGDGETTLNSGV----------- 60
           AAST+DRKFVT V++GNG++FQ  G   ++ ++ Y  +  G    N+G            
Sbjct: 326 AASTMDRKFVTQVQIGNGQSFQ--GVSINTFDNQYYPLVSGRDIPNTGFDKSTSRFCTDK 385

Query: 61  -----------------------------VGTIMQDANDQDVTFIFPRPASKLDLNTGKN 120
                                           ++  +N +D    +P P+S LD N    
Sbjct: 386 SVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLA 445

Query: 121 VSEYLRSTR-------YSDTLLKAY-----------------------LAAQGVDILTSW 169
              Y+ S R        S T+L A                        ++  GV+IL +W
Sbjct: 446 TLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAW 505

[Close]

BLAST of Lcy09g016300 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.9e-17
Identity = 69/212 (32.55%), Postives = 104/212 (49.06%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGET--------FQFFGCE-PSSSEDFYDE-IGDGETT----LN 60
           AA+T +R+F+T V LGNG+T        F   G + P    D+ +E +  G+      L+
Sbjct: 320 AATTTNRRFLTKVVLGNGKTLVGKSVNAFDLKGKKYPLEYGDYLNESLVKGKILVSRYLS 379

Query: 61  SGVVGTIMQDANDQDVTFIFPRPASKLDLNTGKNVSEYLRSTR----------------- 120
              V       +++D   I  RP S L  +   ++  Y+ STR                 
Sbjct: 380 GSEVAVSFITTDNKDYASISSRPLSVLSQDDFDSLVSYINSTRSPQGSVLKTEAIFNQLS 439

Query: 121 -------------YSDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTS 169
                         +  +LK  ++A GV+IL ++S  S  +    D R V + +++SGTS
Sbjct: 440 PKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLPSEDRRDKRRVKY-SVLSGTS 499

[Close]

BLAST of Lcy09g016300 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.9e-17
Identity = 47/80 (58.75%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 89  RYSDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTSMACPHATGVAAY 148
           R S  +LK  ++A G++IL ++S+ ++ T  P DNR   F +I+SGTSMACPHA   AAY
Sbjct: 497 RISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLF-SIMSGTSMACPHAAAAAAY 556

Query: 149 VKSFHRTWSPAAIKSALMTT 169
           VKSFH  WSPAAIKSALMTT
Sbjct: 557 VKSFHPDWSPAAIKSALMTT 575

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BLAST of Lcy09g016300 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.3e-16
Identity = 46/82 (56.10%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 91  SDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTSMACPHATGVAAYVK 150
           S  +LK  L A GV+IL +W++A   T LP D R   F NI+SGTSMACPH +G AA +K
Sbjct: 494 SPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEF-NILSGTSMACPHVSGAAALLK 553

Query: 151 SFHRTWSPAAIKSALMTTVQLV 173
           S H  WSPA I+SA+MTT  LV
Sbjct: 554 SAHPDWSPAVIRSAMMTTTNLV 574

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BLAST of Lcy09g016300 vs. ExPASy TrEMBL
Match: A0A6J1CC81 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010157 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-45
Identity = 119/239 (49.79%), Postives = 133/239 (55.65%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQ-----------------FFGCEPSSSED---------- 60
           AASTIDRKFVT VKLGNG+ F+                 + G  P+S+            
Sbjct: 314 AASTIDRKFVTKVKLGNGKAFEGISVNTFQLEDKMFPLIYAGDAPNSTAGFNGSLSRFCF 373

Query: 61  --------------FYDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                         F DEIGDG T L+SG +GTIMQDAN  DV F+FP PASKLDLN G 
Sbjct: 374 PGSLDMNLVQGKIVFCDEIGDGTTALSSGAIGTIMQDANFLDVAFVFPLPASKLDLNAGS 433

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
            V +YLRST   +                               +LK  L A GVDIL S
Sbjct: 434 KVFQYLRSTSNPEANIEKSTTIDDLSAPFVVSFSSRGPNQITLDILKPDLTAPGVDILAS 493

[Close]

BLAST of Lcy09g016300 vs. ExPASy TrEMBL
Match: A0A6J1JLZ2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111487088 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 8.7e-44
Identity = 119/239 (49.79%), Postives = 131/239 (54.81%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGE--------TFQ---------FFGCEPSSSEDF--------- 60
           AASTIDRKFVTNVKLGNGE        TFQ         + G  P+++  F         
Sbjct: 288 AASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCF 347

Query: 61  ---------------YDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                           DEIGDGE  L+SG VGTIMQD   QD  F+FP PAS LDLN G 
Sbjct: 348 PGSLDVNQVEGKIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGN 407

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           NV +YLRST   +                               +LK  LAA GVDI+ S
Sbjct: 408 NVFQYLRSTSNPEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVAS 467

[Close]

BLAST of Lcy09g016300 vs. ExPASy TrEMBL
Match: A0A6J1GXX0 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458206 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 8.7e-44
Identity = 119/239 (49.79%), Postives = 131/239 (54.81%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGE--------TFQ---------FFGCEPSSSEDF--------- 60
           AASTIDRKFVTNVKLGNGE        TFQ         + G  P+++  F         
Sbjct: 230 AASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNGSLSRFCF 289

Query: 61  ---------------YDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                           DEIGDGE  L+SG VGTIMQD   QD  F+FP PAS LDLN G 
Sbjct: 290 PGSLDVNQVEGKIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGN 349

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           NV +YLRST   +                               +LK  LAA GVDI+ S
Sbjct: 350 NVFQYLRSTSNPEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVAS 409

[Close]

BLAST of Lcy09g016300 vs. ExPASy TrEMBL
Match: A0A0A0KQC8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G188880 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.2e-39
Identity = 111/239 (46.44%), Postives = 127/239 (53.14%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQ----------------------------FFG-----CE 60
           AASTIDRKFVTNV LGNGE+ Q                            F G     C 
Sbjct: 230 AASTIDRKFVTNVALGNGESIQGISVNTFDLGDKLFPLIHAGDAPNTTAGFNGSTSRLCF 289

Query: 61  PSSSED--------FYDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
           P S ++          D I DGE T +SG VGTIMQ+ N QDV F+FP+P S +  NTG+
Sbjct: 290 PGSLDEDKVQGKIVICDLISDGEVTQSSGAVGTIMQNPNFQDVAFLFPQPVSLISFNTGE 349

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
            + +YLRS    +                               +LK  LAA GVDIL S
Sbjct: 350 KLFQYLRSNSNPEAAIEKSTTIEDLSAPAVVSFSSRGPNLITLDILKPDLAAPGVDILAS 409

[Close]

BLAST of Lcy09g016300 vs. ExPASy TrEMBL
Match: A0A0A0KSA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G188380 PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.3e-36
Identity = 108/239 (45.19%), Postives = 122/239 (51.05%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQ----------------------------FFG-----CE 60
           AASTIDRKFVT V LGNGE+F                             F G     C 
Sbjct: 271 AASTIDRKFVTKVTLGNGESFHGISLNTFDAGDKLFPLIHAGEAPNTTAGFNGSISRLCF 330

Query: 61  PSSSE--------DFYDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
           P S +           D I DGE  L SG VGTIMQ +   +V F+FP P S ++ N GK
Sbjct: 331 PGSLDMNKVQGKIVLCDLISDGEAALISGAVGTIMQGSTLPEVAFLFPLPVSLINFNAGK 390

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           N+ +YLRS    +                               +LK  LAA GVDIL S
Sbjct: 391 NIFQYLRSNSNPEAIIEKSTTIEDLSAPSVISFSSRGPNTVTLDILKPDLAASGVDILAS 450

[Close]

BLAST of Lcy09g016300 vs. NCBI nr
Match: XP_022139189.1 (cucumisin-like [Momordica charantia])

HSP 1 Score: 193.4 bits (490), Expect = 2.5e-45
Identity = 119/239 (49.79%), Postives = 133/239 (55.65%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQ-----------------FFGCEPSSSED---------- 60
           AASTIDRKFVT VKLGNG+ F+                 + G  P+S+            
Sbjct: 314 AASTIDRKFVTKVKLGNGKAFEGISVNTFQLEDKMFPLIYAGDAPNSTAGFNGSLSRFCF 373

Query: 61  --------------FYDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                         F DEIGDG T L+SG +GTIMQDAN  DV F+FP PASKLDLN G 
Sbjct: 374 PGSLDMNLVQGKIVFCDEIGDGTTALSSGAIGTIMQDANFLDVAFVFPLPASKLDLNAGS 433

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
            V +YLRST   +                               +LK  L A GVDIL S
Sbjct: 434 KVFQYLRSTSNPEANIEKSTTIDDLSAPFVVSFSSRGPNQITLDILKPDLTAPGVDILAS 493

[Close]

BLAST of Lcy09g016300 vs. NCBI nr
Match: XP_038891640.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 190.3 bits (482), Expect = 2.1e-44
Identity = 121/239 (50.63%), Postives = 131/239 (54.81%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQ----------------------------FFG-----CE 60
           AASTIDRKFVTNVKLGNGE+FQ                            F G     C 
Sbjct: 495 AASTIDRKFVTNVKLGNGESFQGTSVNTFQLGDKLFPLIHAGEAPNTTAGFNGSLSRFCI 554

Query: 61  PSSSE--------DFYDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
           P S +           DEIGDG   L+SG VGTIMQDA  QDV F+FP P S +DL  GK
Sbjct: 555 PGSLDVDKVRGKIVLCDEIGDGGPALSSGAVGTIMQDAGHQDVAFLFPLPVSLIDLKAGK 614

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           NV +YLRS R  +                               +LK  LAA GVDIL S
Sbjct: 615 NVFQYLRSNRNPEATIEKSTTIEDLSAPSVVSFSSRGPNIVTLDILKPDLAAPGVDILAS 674

[Close]

BLAST of Lcy09g016300 vs. NCBI nr
Match: XP_022990086.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-43
Identity = 119/239 (49.79%), Postives = 131/239 (54.81%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGE--------TFQ---------FFGCEPSSSEDF--------- 60
           AASTIDRKFVTNVKLGNGE        TFQ         + G  P+++  F         
Sbjct: 288 AASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCF 347

Query: 61  ---------------YDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                           DEIGDGE  L+SG VGTIMQD   QD  F+FP PAS LDLN G 
Sbjct: 348 PGSLDVNQVEGKIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGN 407

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           NV +YLRST   +                               +LK  LAA GVDI+ S
Sbjct: 408 NVFQYLRSTSNPEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVAS 467

[Close]

BLAST of Lcy09g016300 vs. NCBI nr
Match: XP_022956485.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-43
Identity = 119/239 (49.79%), Postives = 131/239 (54.81%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGE--------TFQ---------FFGCEPSSSEDF--------- 60
           AASTIDRKFVTNVKLGNGE        TFQ         + G  P+++  F         
Sbjct: 230 AASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNGSLSRFCF 289

Query: 61  ---------------YDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                           DEIGDGE  L+SG VGTIMQD   QD  F+FP PAS LDLN G 
Sbjct: 290 PGSLDVNQVEGKIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGN 349

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           NV +YLRST   +                               +LK  LAA GVDI+ S
Sbjct: 350 NVFQYLRSTSNPEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVAS 409

[Close]

BLAST of Lcy09g016300 vs. NCBI nr
Match: XP_023526089.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 186.4 bits (472), Expect = 3.1e-43
Identity = 119/239 (49.79%), Postives = 131/239 (54.81%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGE--------TFQ---------FFGCEPSSSEDF--------- 60
           AASTIDRKFVTNVKLGNGE        TFQ         + G  P+++  F         
Sbjct: 230 AASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCF 289

Query: 61  ---------------YDEIGDGETTLNSGVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
                           DEIGDGE  L+SG VGTIMQD   QD  F+FP PAS LDLN G 
Sbjct: 290 PGSLDVNQVEGKIVLCDEIGDGEGALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGN 349

Query: 121 NVSEYLRSTRYSDT------------------------------LLKAYLAAQGVDILTS 169
           NV +YLRST   +                               +LK  LAA GVDI+ S
Sbjct: 350 NVFQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVAS 409

[Close]

BLAST of Lcy09g016300 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-19
Identity = 76/249 (30.52%), Postives = 109/249 (43.78%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQFFGCE-----------------PSSSED-------FYD 60
           AAS IDR F + + LGNG++F   G                     +++D       F D
Sbjct: 332 AASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTDDKYLARYCFSD 391

Query: 61  EI----------------GDGETTLNS-GVVGTIMQDANDQDVTFIFPRPASKLDLNTGK 120
            +                G  E+T+ S G  G I+      D   IF  PA+ ++ + G 
Sbjct: 392 SLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGD 451

Query: 121 NVSEYLRSTRYSDT-----------------------------LLKAYLAAQGVDILTSW 180
            +  Y+ STR +                               LLK  +AA G+DIL ++
Sbjct: 452 IIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILAAF 511

[Close]

BLAST of Lcy09g016300 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.8e-18
Identity = 47/80 (58.75%), Postives = 59/80 (73.75%), Query Frame = 0


Query: 89  RYSDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTSMACPHATGVAAY 148
           R S  +LK  ++A G++IL ++S+ ++ T  P DNR   F +I+SGTSMACPHA   AAY
Sbjct: 497 RISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLF-SIMSGTSMACPHAAAAAAY 556

Query: 149 VKSFHRTWSPAAIKSALMTT 169
           VKSFH  WSPAAIKSALMTT
Sbjct: 557 VKSFHPDWSPAAIKSALMTT 575

[Close]

BLAST of Lcy09g016300 vs. TAIR 10
Match: AT5G58830.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.8e-18
Identity = 69/212 (32.55%), Postives = 104/212 (49.06%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGET--------FQFFGCE-PSSSEDFYDE-IGDGETT----LN 60
           AA+T +R+F+T V LGNG+T        F   G + P    D+ +E +  G+      L+
Sbjct: 311 AATTTNRRFLTKVVLGNGKTLVGKSVNAFDLKGKKYPLEYGDYLNESLVKGKILVSRYLS 370

Query: 61  SGVVGTIMQDANDQDVTFIFPRPASKLDLNTGKNVSEYLRSTR----------------- 120
              V       +++D   I  RP S L  +   ++  Y+ STR                 
Sbjct: 371 GSEVAVSFITTDNKDYASISSRPLSVLSQDDFDSLVSYINSTRSPQGSVLKTEAIFNQLS 430

Query: 121 -------------YSDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTS 169
                         +  +LK  ++A GV+IL ++S  S  +    D R V + +++SGTS
Sbjct: 431 PKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLPSEDRRDKRRVKY-SVLSGTS 490

[Close]

BLAST of Lcy09g016300 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 86.7 bits (213), Expect = 3.1e-17
Identity = 46/82 (56.10%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 91  SDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTSMACPHATGVAAYVK 150
           S  +LK  L A GV+IL +W++A   T LP D R   F NI+SGTSMACPH +G AA +K
Sbjct: 494 SPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEF-NILSGTSMACPHVSGAAALLK 553

Query: 151 SFHRTWSPAAIKSALMTTVQLV 173
           S H  WSPA I+SA+MTT  LV
Sbjct: 554 SAHPDWSPAVIRSAMMTTTNLV 574

[Close]

BLAST of Lcy09g016300 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 85.1 bits (209), Expect = 9.0e-17
Identity = 66/212 (31.13%), Postives = 99/212 (46.70%), Query Frame = 0


Query: 1   AASTIDRKFVTNVKLGNGETFQFFGCE----PSSSEDFYDEIGDG----------ETTLN 60
           AAS  +R FVT V LGNG+TF             +   Y    DG          E  ++
Sbjct: 322 AASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYPLYGGSTDGPLLRGKILVSEDKVS 381

Query: 61  SGVVGTIMQDANDQDVTFIFPRPASKLDLNTGKNVSEYLRSTR----------------- 120
           S +V   + + N  D  ++   P+S L  +   +V  Y+ ST+                 
Sbjct: 382 SEIVVANINE-NYHDYAYVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAA 441

Query: 121 -------------YSDTLLKAYLAAQGVDILTSWSEASTATSLPWDNRIVPFINIISGTS 169
                         +  +LK  + A GV+IL ++S  ++      DNR V + +++SGTS
Sbjct: 442 PKVAGFSSRGPNTIAVDILKPDVTAPGVEILAAFSPLNSPAQDKRDNRHVKY-SVLSGTS 501

The following BLAST results are available for this feature:

BLAST of Lcy09g016300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LLL8	1.6e-18	30.52	Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2...	[more]
Q39547	2.7e-18	30.67	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIM6	3.9e-17	32.55	Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 S...	[more]
Q9LZS6	3.9e-17	58.75	Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3...	[more]
O49607	4.3e-16	56.10	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]

BLAST of Lcy09g016300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CC81	1.2e-45	49.79	cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010157 PE=3 SV=1	[more]
A0A6J1JLZ2	8.7e-44	49.79	cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111487088 PE=3 SV=1	[more]
A0A6J1GXX0	8.7e-44	49.79	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458206 PE=3 SV=1	[more]
A0A0A0KQC8	2.2e-39	46.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G188880 PE=3 SV=1	[more]
A0A0A0KSA0	1.3e-36	45.19	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G188380 PE=3 SV=1	[more]

BLAST of Lcy09g016300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139189.1	2.5e-45	49.79	cucumisin-like [Momordica charantia]	[more]
XP_038891640.1	2.1e-44	50.63	cucumisin-like [Benincasa hispida]	[more]
XP_022990086.1	1.8e-43	49.79	cucumisin-like [Cucurbita maxima]	[more]
XP_022956485.1	1.8e-43	49.79	cucumisin-like [Cucurbita moschata]	[more]
XP_023526089.1	3.1e-43	49.79	cucumisin-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy09g016300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G00230.1	1.1e-19	30.52	xylem serine peptidase 1	[more]
AT5G03620.1	2.8e-18	58.75	Subtilisin-like serine endopeptidase family protein	[more]
AT5G58830.1	2.8e-18	32.55	Subtilisin-like serine endopeptidase family protein	[more]
AT4G34980.1	3.1e-17	56.10	subtilisin-like serine protease 2	[more]
AT5G58840.1	9.0e-17	31.13	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 77..169 e-value: 3.4E-13 score: 49.5
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 92..172 e-value: 5.7E-24 score: 86.7
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 79..174
None	No IPR available	PANTHER	PTHR10795:SF375	CUCUMISIN-LIKE	coord: 93..173
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 93..173
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 134..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g016300.1	Lcy09g016300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity