Lcy09g013640 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g013640
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr09: 15212122 .. 15214602 (-)
RNA-Seq ExpressionLcy09g013640
SyntenyLcy09g013640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTGACCCACTTCTCTGATTTTGATGATTTTTCCCTGGGCAAGATTTGATTGAATGTGAAGTTCTGTAGAAATGTTCCTCGGCTGTTTAAATCTTTTAGATCCATGGTGGGGTTTCTTTGAATTTGAGGGTGTACTTTGCTTTTTTTGTTTTTTAAATTGTGTGTTCAGATTATGCAATTAGTGAAGATTTTATCATTTCATATTCTTCAGGGGAAGGGAATCCATGAATCCATGTGCTTATCGCCGTCTCTTGTCTCGTTTCATGACGTGCAAATTTATGGTTTCCATTAATAAAATACTGATTTCAACCTAGGATTCTTTTTCGGAATTCTCTTCATACAGAGTCTGTTTTGTTTTGCATGTTATCTGTTGTACACTCTACATGGCAGTTCTGAATTCAAATTTTTTTTAAGAGAAGATTGATTCTGAAACCACTTGTTGTGCATATGTTCAGGTAGTATAAAACTGTCCTTTCAAGGATGGCATCTGGAAGTCCTTATTTTGATGATATGCGGAGCAAACCAGAGGTCATTGATCCTCCCCAAAATGAAGATATGATGGACATCAGGGAAACTGTGGATGATCCAGCTCAAAATGAACTTAAACCTAATGTTACTGTTTCCAGCAGCGTTCGTGAGCTTTTGGAATGCCCGGTGTGTTTGAATGCAATGTACCCTCCAATACATCAGGTTTGGTTATGAATTATTTAATTCATCTTTATGTGATGAGGTTAACTTTGCAGACTGATGATGCCCACAGGATTTTGGTGTTGATGTAGTTGTTAGAGATTCTATGTCTGTGAAGGTTTAGTAATAGTTAGAATGAATTTATTACCAAGTTTTGTACACTTTGATCTCTTTTTATATGTTCTGTACATTCCAGAAGGCATGTGTTTAAGCTTATGGGTGACAGCAGTTTCTTTAATGGTGTTGTTTGTCACTGCAGTGTTCAAATGGTCACACACTGTGTTCTGGTTGCAAGCCTAGGGTGCACAATCGGTGTCCTACGTGTAGGCACGAACTTGGCAATATCAGATGTCTGGCGTTGGAGAAGGTGGCCGCATCTCTAGAACTTCCGTGTAAATATCAGAGCTATGGTTGCATTGGCATATATCCGTATTACAGCAAGGTCAAACATGAATCCCAATGTGTTTATAGACCTTATAGTTGTCCATATGCTGGATCAGAATGTTCAGTCGTTGGTGATATACCCTATTTGGTGGCCCACCTGAAAGATGATCATAAAGTAGACATGCACAGTGGAAGCACATTCAACCATCGTTATGTAAAATCAAACCCTCAAGAAGTTGAAAATGCAACATGGATGCTAACGGTAATTTTCCTCGAAAACTTTTGTTTCAGTTCTCAAGCAGCCCACTCTGTTCCTTACCCTTTTGGGCAGGCAAATATCTTTAGCATTTCTTATGCACATTTTGTGCGTTAGAATCATGTTAGATACATAAATTTTAGTGATTTGATCTTGGTATGAAACCATGCACTTCTTTGGAGTTCTCCTGTTCATGGAATTGGGATGTCGTTTGATGGATGGCTAAACAATTTAGGATATATCAGAAATTGAGCAGAAGAATGCTCACTTTGGTTTTGCTATCATACGTTAGGAAATGTACTCCTGTATATTAGAAATATTTCTGATTTGTTTACTGAGCTCGGGCCTTGGAGTACCTCAAGTTAAAAAGATTTTGCTAGTTTAGAAATCTAGGATCCCCATTTTACCATCGTTCTGCAAACCCATAGCTTATATTAACTTTTGAATTCTTGATTGTGGAGTGAATCTGATTTAGAATTTTAAAATTCAGGTTTTCAGTTGCTTTGGTCAATACTTTTGCTTGCATTTCGAAGCTTTCCAGCTCGGAATGGCTCCAGTCTACATAGCTTTTTTGCGGTTCATGGGTGATGATAATGATGCAAAAAACTACAGCTATAGTCTCGAGGTGGGCGGAAATGGGAGGAAGATGGTATGGCAAGGGGTACCCAGAAGCATCAGGGACAGCCATCGGAAAGTTCGGGACAGTTTTGATGGGCTCATTATCCAACGCAATATGGCGCTCTTCTTCTCCGGTGGCGACCGGAAGGAACTAAAGCTTAGGGTGACTGGTAGAATTTGGAAAGAACAGTGATACTGAGAGAACTGTGCTTATGAACTTTCAGTTCAAATCCTATCTCCTTGGCCAGTAGGTTGGTTGCCTTTTTGTAGAGTAAATGAGGCTTTCTAATCATGTGTTTCCTTTTTAATTAAGATGCAAACACTCTTTGCATGAAACCATGATTTAGAAAAGAACTTCCCCCCATTGATGATTATTGTATGCAGAACTAGAACTTATTCCTCATATTTTCTTGTTGAGTATTAACAATTTAAGTTACCTCATTGTTAGAGATGCAGCCATGGCTTCATATGATGTTCAGTCCTTGGATCCACATTATAAAATTTATGAAATGATAATTATAAGCTTAAATTTTT

mRNA sequence

ATTTTGACCCACTTCTCTGATTTTGATGATTTTTCCCTGGGCAAGATTTGATTGAATGTGAAGTTCTGTAGAAATGTTCCTCGGCTGTTTAAATCTTTTAGATCCATGGGGAAGGGAATCCATGAATCCATGTGCTTATCGCCGTCTCTTGTCTCGTTTCATGACGTGCAAATTTATGGTTTCCATTAATAAAATACTGATTTCAACCTAGGATTCTTTTTCGGAATTCTCTTCATACAGAGTCTGTTTTGTTTTGCATGTTATCTGTTGTACACTCTACATGGCAGTTCTGAATTCAAATTTTTTTTAAGAGAAGATTGATTCTGAAACCACTTGTTGTGCATATGTTCAGGTAGTATAAAACTGTCCTTTCAAGGATGGCATCTGGAAGTCCTTATTTTGATGATATGCGGAGCAAACCAGAGGTCATTGATCCTCCCCAAAATGAAGATATGATGGACATCAGGGAAACTGTGGATGATCCAGCTCAAAATGAACTTAAACCTAATGTTACTGTTTCCAGCAGCGTTCGTGAGCTTTTGGAATGCCCGGTGTGTTTGAATGCAATGTACCCTCCAATACATCAGTGTTCAAATGGTCACACACTGTGTTCTGGTTGCAAGCCTAGGGTGCACAATCGGTGTCCTACGTGTAGGCACGAACTTGGCAATATCAGATGTCTGGCGTTGGAGAAGGTGGCCGCATCTCTAGAACTTCCGTGTAAATATCAGAGCTATGGTTGCATTGGCATATATCCGTATTACAGCAAGGTCAAACATGAATCCCAATGTGTTTATAGACCTTATAGTTGTCCATATGCTGGATCAGAATGTTCAGTCGTTGGTGATATACCCTATTTGGTGGCCCACCTGAAAGATGATCATAAAGTAGACATGCACAGTGGAAGCACATTCAACCATCGTTATGTAAAATCAAACCCTCAAGAAGTTGAAAATGCAACATGGATGCTAACGGTTTTCAGTTGCTTTGGTCAATACTTTTGCTTGCATTTCGAAGCTTTCCAGCTCGGAATGGCTCCAGTCTACATAGCTTTTTTGCGGTTCATGGGTGATGATAATGATGCAAAAAACTACAGCTATAGTCTCGAGGTGGGCGGAAATGGGAGGAAGATGGTATGGCAAGGGGTACCCAGAAGCATCAGGGACAGCCATCGGAAAGTTCGGGACAGTTTTGATGGGCTCATTATCCAACGCAATATGGCGCTCTTCTTCTCCGGTGGCGACCGGAAGGAACTAAAGCTTAGGGTGACTGGTAGAATTTGGAAAGAACAGTGATACTGAGAGAACTGTGCTTATGAACTTTCAGTTCAAATCCTATCTCCTTGGCCAGTAGGTTGGTTGCCTTTTTGTAGAGTAAATGAGGCTTTCTAATCATGTGTTTCCTTTTTAATTAAGATGCAAACACTCTTTGCATGAAACCATGATTTAGAAAAGAACTTCCCCCCATTGATGATTATTGTATGCAGAACTAGAACTTATTCCTCATATTTTCTTGTTGAGTATTAACAATTTAAGTTACCTCATTGTTAGAGATGCAGCCATGGCTTCATATGATGTTCAGTCCTTGGATCCACATTATAAAATTTATGAAATGATAATTATAAGCTTAAATTTTT

Coding sequence (CDS)

ATGGCATCTGGAAGTCCTTATTTTGATGATATGCGGAGCAAACCAGAGGTCATTGATCCTCCCCAAAATGAAGATATGATGGACATCAGGGAAACTGTGGATGATCCAGCTCAAAATGAACTTAAACCTAATGTTACTGTTTCCAGCAGCGTTCGTGAGCTTTTGGAATGCCCGGTGTGTTTGAATGCAATGTACCCTCCAATACATCAGTGTTCAAATGGTCACACACTGTGTTCTGGTTGCAAGCCTAGGGTGCACAATCGGTGTCCTACGTGTAGGCACGAACTTGGCAATATCAGATGTCTGGCGTTGGAGAAGGTGGCCGCATCTCTAGAACTTCCGTGTAAATATCAGAGCTATGGTTGCATTGGCATATATCCGTATTACAGCAAGGTCAAACATGAATCCCAATGTGTTTATAGACCTTATAGTTGTCCATATGCTGGATCAGAATGTTCAGTCGTTGGTGATATACCCTATTTGGTGGCCCACCTGAAAGATGATCATAAAGTAGACATGCACAGTGGAAGCACATTCAACCATCGTTATGTAAAATCAAACCCTCAAGAAGTTGAAAATGCAACATGGATGCTAACGGTTTTCAGTTGCTTTGGTCAATACTTTTGCTTGCATTTCGAAGCTTTCCAGCTCGGAATGGCTCCAGTCTACATAGCTTTTTTGCGGTTCATGGGTGATGATAATGATGCAAAAAACTACAGCTATAGTCTCGAGGTGGGCGGAAATGGGAGGAAGATGGTATGGCAAGGGGTACCCAGAAGCATCAGGGACAGCCATCGGAAAGTTCGGGACAGTTTTGATGGGCTCATTATCCAACGCAATATGGCGCTCTTCTTCTCCGGTGGCGACCGGAAGGAACTAAAGCTTAGGGTGACTGGTAGAATTTGGAAAGAACAGTGA

Protein sequence

MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ
Homology
BLAST of Lcy09g013640 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 3.9e-157
Identity = 259/299 (86.62%), Postives = 275/299 (91.97%), Query Frame = 0

Query: 7   YFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVCLNAMYP 66
           Y DD  S  EVIDPP+ E ++D+ E   DP  +  KPNV VSSSVRELLECPVCL+AMYP
Sbjct: 6   YIDD--SGSEVIDPPKTE-VLDVTELAGDPVPHSPKPNVVVSSSVRELLECPVCLSAMYP 65

Query: 67  PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSYGCIGIY 126
           PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQ++GC+GIY
Sbjct: 66  PIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQNFGCVGIY 125

Query: 127 PYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFNHRYVKS 186
           PYY K+KHESQC YRPYSCPYAGSEC+V GDIPYLV HLKDDHKVDMH+G TFNHRYVKS
Sbjct: 126 PYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDDHKVDMHNGCTFNHRYVKS 185

Query: 187 NPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYSYSLEVG 246
           NP EVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDD +AKNYSYSLEVG
Sbjct: 186 NPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDLEAKNYSYSLEVG 245

Query: 247 GNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           G GRKM+WQGVPRSIRDSHRKVRDS+DGLIIQRNMALFFSGG+RKELKLRVTGRIWKEQ
Sbjct: 246 GTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of Lcy09g013640 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 486.1 bits (1250), Expect = 2.9e-136
Identity = 217/261 (83.14%), Postives = 242/261 (92.72%), Query Frame = 0

Query: 45  VTVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLAL 104
           ++ ++SV ELLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLAL
Sbjct: 34  ISPATSVYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLAL 93

Query: 105 EKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAH 164
           EKVA SLELPCKY + GC+GI+PYYSK+KHESQC +RPYSCPYAGSEC+ VGDI +LVAH
Sbjct: 94  EKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAH 153

Query: 165 LKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYI 224
           L+DDHKVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+
Sbjct: 154 LRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYM 213

Query: 225 AFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALF 284
           AFLRFMGD++DA+NY+YSLEVGG+GRK  W+G PRS+RDSHRKVRDS DGLIIQRNMALF
Sbjct: 214 AFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALF 273

Query: 285 FSGGDRKELKLRVTGRIWKEQ 306
           FSGGD+KELKLRVTGRIWKEQ
Sbjct: 274 FSGGDKKELKLRVTGRIWKEQ 294

BLAST of Lcy09g013640 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.2e-135
Identity = 217/258 (84.11%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           ++SV ELLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 54  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 113

Query: 108 AASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKD 167
           A SLELPCK+ S GC  I+PYYSK+KHE+ C +RPYSCPYAGSECSV GDIP+LVAHL+D
Sbjct: 114 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 173

Query: 168 DHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMHSG TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFL
Sbjct: 174 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 233

Query: 228 RFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+ +A+NY+YSLEVGG GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSG
Sbjct: 234 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 293

Query: 288 GDRKELKLRVTGRIWKEQ 306
           GDRKELKLRVTGRIWKEQ
Sbjct: 294 GDRKELKLRVTGRIWKEQ 311

BLAST of Lcy09g013640 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 2.3e-133
Identity = 218/268 (81.34%), Postives = 241/268 (89.93%), Query Frame = 0

Query: 39  NELKPNVTV-SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELG 98
           N + P  T  ++SV ELLECPVC  +MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG
Sbjct: 45  NIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELG 104

Query: 99  NIRCLALEKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGD 158
           +IRCLALEKVA SLELPCK+ + GC  I+PYYSK+KHES C +RPYSCPYAGSEC +VGD
Sbjct: 105 DIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGD 164

Query: 159 IPYLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQL 218
           IP+LVAHL+DDHKVDMH+GSTFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQL
Sbjct: 165 IPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQL 224

Query: 219 GMAPVYIAFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLII 278
           GM PVY+AFLRFMGD+ DA++YSYSLEVGG+GRK+ W+G PRSIRDSHRKVRDS DGLII
Sbjct: 225 GMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLII 284

Query: 279 QRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           QRNMALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 285 QRNMALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of Lcy09g013640 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 7.6e-129
Identity = 208/264 (78.79%), Postives = 232/264 (87.88%), Query Frame = 0

Query: 42  KPNVTVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRC 101
           K  +  ++ V ELLECPVC N MYPPIHQC NGHTLCS CK RV N CPTCR+ELGNIRC
Sbjct: 45  KYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRC 104

Query: 102 LALEKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYL 161
           LALEKVA SLE+PC+YQ+ GC  I+PYYSK+KHE  C +RPY+CPYAGSECSV GDIP L
Sbjct: 105 LALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTL 164

Query: 162 VAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAP 221
           V HLKDDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAP
Sbjct: 165 VVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAP 224

Query: 222 VYIAFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 281
           VY+AFLRFMGD+N+AK +SYSLEVG +GRK+ WQG+PRSIRDSHRKVRDS DGLII RN+
Sbjct: 225 VYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNL 284

Query: 282 ALFFSGGDRKELKLRVTGRIWKEQ 306
           AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 285 ALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Lcy09g013640 vs. ExPASy TrEMBL
Match: A0A6J1FG80 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445421 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 1   MASGGPYFDDLRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Lcy09g013640 vs. ExPASy TrEMBL
Match: A0A6J1FN07 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445421 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 38  MASGGPYFDDLRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 97

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 98  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 157

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 158 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 217

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 218 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 277

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 278 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 337

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 338 IWKEQ 342

BLAST of Lcy09g013640 vs. ExPASy TrEMBL
Match: A0A6J1JWI5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488979 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.5e-180
Identity = 299/305 (98.03%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPE+IDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 1   MASGGPYFDDLRSKPEIIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Lcy09g013640 vs. ExPASy TrEMBL
Match: A0A6J1JYA8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488979 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.5e-180
Identity = 299/305 (98.03%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPE+IDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 38  MASGGPYFDDLRSKPEIIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 97

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 98  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 157

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 158 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 217

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 218 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 277

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 278 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 337

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 338 IWKEQ 342

BLAST of Lcy09g013640 vs. ExPASy TrEMBL
Match: A0A1S3CP93 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503267 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 9.8e-180
Identity = 299/305 (98.03%), Postives = 301/305 (98.69%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASGSPYFDD+RSKPEVIDPPQNEDMMDIRETVD P QNELKPNV VSSSVRELLECPVC
Sbjct: 1   MASGSPYFDDLRSKPEVIDPPQNEDMMDIRETVDHPIQNELKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKY +Y
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYHTY 120

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Lcy09g013640 vs. NCBI nr
Match: XP_038906777.1 (E3 ubiquitin-protein ligase DIS1-like [Benincasa hispida] >XP_038906778.1 E3 ubiquitin-protein ligase DIS1-like [Benincasa hispida])

HSP 1 Score: 643.3 bits (1658), Expect = 1.1e-180
Identity = 301/305 (98.69%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASGSPYFDD+RSKPEVIDPPQNEDMMDIRETVDDP QNELKPNV VSSSVRELLECPVC
Sbjct: 1   MASGSPYFDDLRSKPEVIDPPQNEDMMDIRETVDDPVQNELKPNVAVSSSVRELLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKY SY
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYHSY 120

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Lcy09g013640 vs. NCBI nr
Match: XP_022939545.1 (E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 642.1 bits (1655), Expect = 2.4e-180
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 38  MASGGPYFDDLRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 97

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 98  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 157

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 158 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 217

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 218 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 277

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 278 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 337

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 338 IWKEQ 342

BLAST of Lcy09g013640 vs. NCBI nr
Match: XP_023550149.1 (E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.1 bits (1655), Expect = 2.4e-180
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 38  MASGGPYFDDLRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 97

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 98  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 157

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 158 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 217

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 218 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 277

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 278 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 337

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 338 IWKEQ 342

BLAST of Lcy09g013640 vs. NCBI nr
Match: XP_022939546.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita moschata] >XP_022939547.1 E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita moschata] >XP_022939548.1 E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita moschata] >XP_023550150.1 E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023550151.1 E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023550152.1 E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.1 bits (1655), Expect = 2.4e-180
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 1   MASGGPYFDDLRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Lcy09g013640 vs. NCBI nr
Match: XP_022992725.1 (E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita maxima] >XP_022992726.1 E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 641.7 bits (1654), Expect = 3.1e-180
Identity = 299/305 (98.03%), Postives = 302/305 (99.02%), Query Frame = 0

Query: 1   MASGSPYFDDMRSKPEVIDPPQNEDMMDIRETVDDPAQNELKPNVTVSSSVRELLECPVC 60
           MASG PYFDD+RSKPE+IDPPQNEDMMDIRETVDDPAQNELKPNV VSSSVR+LLECPVC
Sbjct: 1   MASGGPYFDDLRSKPEIIDPPQNEDMMDIRETVDDPAQNELKPNVAVSSSVRDLLECPVC 60

Query: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120
           LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY
Sbjct: 61  LNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVAASLELPCKYQSY 120

Query: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180
           GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN
Sbjct: 121 GCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKDDHKVDMHSGSTFN 180

Query: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240
           HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS
Sbjct: 181 HRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNDAKNYS 240

Query: 241 YSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300
           Y LEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR
Sbjct: 241 YRLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGR 300

Query: 301 IWKEQ 306
           IWKEQ
Sbjct: 301 IWKEQ 305

BLAST of Lcy09g013640 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 482.3 bits (1240), Expect = 3.0e-136
Identity = 217/258 (84.11%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           ++SV ELLECPVC N+MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 54  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 113

Query: 108 AASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKD 167
           A SLELPCK+ S GC  I+PYYSK+KHE+ C +RPYSCPYAGSECSV GDIP+LVAHL+D
Sbjct: 114 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 173

Query: 168 DHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMHSG TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFL
Sbjct: 174 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 233

Query: 228 RFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+ +A+NY+YSLEVGG GRK++W+G PRS+RDSHRKVRDS DGLIIQRNMALFFSG
Sbjct: 234 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 293

Query: 288 GDRKELKLRVTGRIWKEQ 306
           GDRKELKLRVTGRIWKEQ
Sbjct: 294 GDRKELKLRVTGRIWKEQ 311

BLAST of Lcy09g013640 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 476.5 bits (1225), Expect = 1.6e-134
Identity = 218/268 (81.34%), Postives = 241/268 (89.93%), Query Frame = 0

Query: 39  NELKPNVTV-SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELG 98
           N + P  T  ++SV ELLECPVC  +MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG
Sbjct: 45  NIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELG 104

Query: 99  NIRCLALEKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGD 158
           +IRCLALEKVA SLELPCK+ + GC  I+PYYSK+KHES C +RPYSCPYAGSEC +VGD
Sbjct: 105 DIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGD 164

Query: 159 IPYLVAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQL 218
           IP+LVAHL+DDHKVDMH+GSTFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQL
Sbjct: 165 IPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQL 224

Query: 219 GMAPVYIAFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLII 278
           GM PVY+AFLRFMGD+ DA++YSYSLEVGG+GRK+ W+G PRSIRDSHRKVRDS DGLII
Sbjct: 225 GMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLII 284

Query: 279 QRNMALFFSGGDRKELKLRVTGRIWKEQ 306
           QRNMALFFSGGDRKELKLRVTG+IWKEQ
Sbjct: 285 QRNMALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of Lcy09g013640 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 461.5 bits (1186), Expect = 5.4e-130
Identity = 208/264 (78.79%), Postives = 232/264 (87.88%), Query Frame = 0

Query: 42  KPNVTVSSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRC 101
           K  +  ++ V ELLECPVC N MYPPIHQC NGHTLCS CK RV N CPTCR+ELGNIRC
Sbjct: 45  KYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRC 104

Query: 102 LALEKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYL 161
           LALEKVA SLE+PC+YQ+ GC  I+PYYSK+KHE  C +RPY+CPYAGSECSV GDIP L
Sbjct: 105 LALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTL 164

Query: 162 VAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAP 221
           V HLKDDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAP
Sbjct: 165 VVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAP 224

Query: 222 VYIAFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 281
           VY+AFLRFMGD+N+AK +SYSLEVG +GRK+ WQG+PRSIRDSHRKVRDS DGLII RN+
Sbjct: 225 VYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNL 284

Query: 282 ALFFSGGDRKELKLRVTGRIWKEQ 306
           AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 285 ALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Lcy09g013640 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 454.1 bits (1167), Expect = 8.6e-128
Identity = 205/258 (79.46%), Postives = 227/258 (87.98%), Query Frame = 0

Query: 48  SSSVRELLECPVCLNAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 107
           S+ V ELLECPVC N MYPPIHQC NGHTLCS CK RV N CPTCR+ELGNIRCLALEKV
Sbjct: 48  SNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKV 107

Query: 108 AASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYLVAHLKD 167
           A SLE+PC+YQ+ GC  I+PYYSK+KHE  C +R YSCPYAGSECSV GDIP LV HLKD
Sbjct: 108 AESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKD 167

Query: 168 DHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 227
           DHKVDMH G TFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVY+AFL
Sbjct: 168 DHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFL 227

Query: 228 RFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNMALFFSG 287
           RFMGD+N+AK +SYSLEVG + RK+ WQG+PRSIRDSHRKVRDS DGLII RN+AL+FSG
Sbjct: 228 RFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSG 287

Query: 288 GDRKELKLRVTGRIWKEQ 306
            D++ELKLRVTGRIWKE+
Sbjct: 288 SDKEELKLRVTGRIWKEE 305

BLAST of Lcy09g013640 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 383.6 bits (984), Expect = 1.4e-106
Identity = 172/204 (84.31%), Postives = 192/204 (94.12%), Query Frame = 0

Query: 102 LALEKVAASLELPCKYQSYGCIGIYPYYSKVKHESQCVYRPYSCPYAGSECSVVGDIPYL 161
           +ALEKVA SLELPCKY + GC+GI+PYYSK+KHESQC +RPYSCPYAGSEC+ VGDI +L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 162 VAHLKDDHKVDMHSGSTFNHRYVKSNPQEVENATWMLTVFSCFGQYFCLHFEAFQLGMAP 221
           VAHL+DDHKVDMH+G TFNHRYVKSNP+EVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 222 VYIAFLRFMGDDNDAKNYSYSLEVGGNGRKMVWQGVPRSIRDSHRKVRDSFDGLIIQRNM 281
           VY+AFLRFMGD++DA+NY+YSLEVGG+GRK  W+G PRS+RDSHRKVRDS DGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 282 ALFFSGGDRKELKLRVTGRIWKEQ 306
           ALFFSGGD+KELKLRVTGRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10L913.9e-15786.62E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q8S3N12.9e-13683.14E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q84JL34.2e-13584.11E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN82.3e-13381.34E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q9M2P47.6e-12978.79E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FG801.2e-18098.36RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445421... [more]
A0A6J1FN071.2e-18098.36RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445421... [more]
A0A6J1JWI51.5e-18098.03RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488979 P... [more]
A0A6J1JYA81.5e-18098.03RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488979 P... [more]
A0A1S3CP939.8e-18098.03RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503267 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_038906777.11.1e-18098.69E3 ubiquitin-protein ligase DIS1-like [Benincasa hispida] >XP_038906778.1 E3 ubi... [more]
XP_022939545.12.4e-18098.36E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita moschata][more]
XP_023550149.12.4e-18098.36E3 ubiquitin-protein ligase DIS1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022939546.12.4e-18098.36E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita moschata] >XP_022939... [more]
XP_022992725.13.1e-18098.03E3 ubiquitin-protein ligase DIS1-like isoform X2 [Cucurbita maxima] >XP_02299272... [more]
Match NameE-valueIdentityDescription
AT3G61790.13.0e-13684.11Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.11.6e-13481.34Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.15.4e-13078.79seven in absentia of Arabidopsis 2 [more]
AT2G41980.18.6e-12879.46Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.11.4e-10684.31TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 110..165
e-value: 2.1E-16
score: 61.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 17..109
e-value: 6.8E-9
score: 37.4
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 99..298
e-value: 3.1E-79
score: 265.6
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 166..298
e-value: 1.2E-38
score: 133.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 7..305
NoneNo IPR availablePANTHERPTHR10315:SF77E3 UBIQUITIN-PROTEIN LIGASE DIS1coord: 7..305
NoneNo IPR availableCDDcd03829Sinacoord: 171..299
e-value: 5.28901E-65
score: 198.38
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 55..93
e-value: 4.00834E-15
score: 66.1542
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 110..302
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 49..97
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 110..170
score: 14.057522
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 57..93
score: 9.346889

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g013640.1Lcy09g013640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding