Lcy09g013340 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g013340
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr09: 14862674 .. 14864886 (+)
RNA-Seq Expression	Lcy09g013340
Synteny	Lcy09g013340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAGACGATAGCAATCAGTATTCTGCTAGAACTCTCTCTCTCTCTATCAAGAATTTGTTTAATAATGTAGAGAGAGAGAGGTGTATTCCAGCATGCATGCACCCTGTAGTCAACATAAACATAATACAAAACTTAAAATAAAAAAACAGGCCAAAAAGACTATGTACCAACAAAGTCCATCAATCACAAGCATTAAGAAAAACACACATTTTTATTTCTATACATCTATCTATCTATCTCTACATATATATATAGACACACACACACACCAACTTCAGCTTCAGCTTCTTCTATATTTTCTTTGTACAGAGACACTCACAAAGCAGCAACTCAATTATACTCCAAACCCCACAACTCTCTCTTTTTCCCTCCTCATATTTCCCACACTTCAATCCATCATTTCTCATTTTCATTCTCAATCTCCTCTCACACCTTAACTACTAAACCATGCTACTCTTCCTCCTCCTTCTTCTTCCTCTTTTAGCAACCGCCGTCGACCCGGTCGCCACCGGCCTCGCCGCTACTTACCCGGCCACCCAACTCCTAAACGTCAAAGACACAATCAAAGAAACAGAAACCAAGCCCTCTAGACTACCACAAGATCTCGACCTCCCTGAAAACAACCTTCCTCTTGACAACAGCAGCAGTCAGAGTCAATGGAAGCTCAAGCTCTTCCACAGAGATAAGCTACCCCTCAACTTCGACCCCGACCACCGTCGCCGTTTCAAGGAGCGTATCGGAAGAGACGTCGAGAGGGTCTCCTCTTTGCTCCGCCAACTCTCCAATGGCAGCGACGAGCAGGTAATCAGGGGCGAAGCGAAGAAGGAAGGAGAGTGACAATTGCCCTCTGAATTTATATAAAGTGCTCAAGAGTTTACAGTTTTTCCTTTGAATGACTTTTGAAAGATTATATAAAAAGTTTGAATGTAAAATTTTGTCTCTTCCTAATAAGAATTTTACTCTGCCTTGCAGGTGACGGACTTTGGGTCGGACGTGGTCTCCGGCACGGAGCAGGGTAGCGGAGAGTACTTTGTGAGGATCGGCGTCGGCAGCCCGCCAAGGAGCCAATACGTCGTAATTGATTCCGGCAGCGACATCGTTTGGGTGCAATGCCAGCCCTGCAGCGAGTGCTACCAACAGTCCGACCCGGTGTTCGACCCGGCCGGCTCCGCCTCCTACGCCGGAGTCTCCTGTGACTCTTCGGTGTGCGACCGCCTCGACAACGCCGGCTGCAACAACGGCCGGTGCCGGTACGAGGTATCGTACGGCGACGGGTCCTACACCCGCGGAACTCTCGCTCTCGAAACCCTAACTTTCGGCCGCGTCGTAATCCGAAACATCGCGATCGGTTGCGGCCATATGAACCGAGGAATGTTCGTCGGAGCCGCCGGATTGCTTGGACTTGGCGGCGGCGCCATGTCGTTCGTAGGCCAGCTCGGCGGCCAAACCGGCGGTGCGTTCAGCTACTGCTTGGTGAGTCGAGGCATCGAGTCCACCGGAACGCTGGAGTTCGGCCGCGGCGCCATGCCGGTCGGCGGCGCGTGGGTTCCCCTAATCCGAAACCCACGCGCTCCCAGTTTCTACTACGTCGGGCTTTCAGGTCTCGGAGTCGGAGGGATCCGAGTTCCAATACCCGAACAGATCTTCGAACTCAAAGAACTAGGATACGGCGGCGTCGTAATGGACACCGGCACCGCCGTAACGAGGCTACCGGCGCCCGCATACGAAGCATTTCGAGACACCTTCATCGAACAAACGGCGAACCTGCCTCGATCAGGCGGAGTATCGATCTTCGACACATGCTATGACCTAAACGGATTCGTATCGGTGAGGGTACCGACGGTGTCGTTCTACTTCTCCGGCGGGCCGATACTGACTTTGCCGGCGAGGAACTTTCTGATTCCGGTGGACGGGGAGGGAACATTTTGCTTTGCATTTGCAGCATCGGCGTCGGAATTGTCGATCATAGGGAACATCCAACAAGAAGGGATTCAAATCTCCGTTGATGGAGCAAATGGGTTTGTGGGATTTGGTCCAAGTATTTGTTAATTCATCAAAACTCAAAGGGCAAATCTGTCATTTTCAGTAGTCTTACATAGTATAAATTCTCTTTGCCTACTTATTTTATTATAATCTATTTTTTATTAGGTTAAATTATAGCTTTAATTTTAAAGTAAATCCCTCACTTTTAAAAAGTGTATAATGTATCATT

mRNA sequence

ATAAGACGATAGCAATCAGTATTCTGCTAGAACTCTCTCTCTCTCTATCAAGAATTTGTTTAATAATGTAGAGAGAGAGAGGTGTATTCCAGCATGCATGCACCCTGTAGTCAACATAAACATAATACAAAACTTAAAATAAAAAAACAGGCCAAAAAGACTATGTACCAACAAAGTCCATCAATCACAAGCATTAAGAAAAACACACATTTTTATTTCTATACATCTATCTATCTATCTCTACATATATATATAGACACACACACACACCAACTTCAGCTTCAGCTTCTTCTATATTTTCTTTGTACAGAGACACTCACAAAGCAGCAACTCAATTATACTCCAAACCCCACAACTCTCTCTTTTTCCCTCCTCATATTTCCCACACTTCAATCCATCATTTCTCATTTTCATTCTCAATCTCCTCTCACACCTTAACTACTAAACCATGCTACTCTTCCTCCTCCTTCTTCTTCCTCTTTTAGCAACCGCCGTCGACCCGGTCGCCACCGGCCTCGCCGCTACTTACCCGGCCACCCAACTCCTAAACGTCAAAGACACAATCAAAGAAACAGAAACCAAGCCCTCTAGACTACCACAAGATCTCGACCTCCCTGAAAACAACCTTCCTCTTGACAACAGCAGCAGTCAGAGTCAATGGAAGCTCAAGCTCTTCCACAGAGATAAGCTACCCCTCAACTTCGACCCCGACCACCGTCGCCGTTTCAAGGAGCGTATCGGAAGAGACGTCGAGAGGGTCTCCTCTTTGCTCCGCCAACTCTCCAATGGCAGCGACGAGCAGGTGACGGACTTTGGGTCGGACGTGGTCTCCGGCACGGAGCAGGGTAGCGGAGAGTACTTTGTGAGGATCGGCGTCGGCAGCCCGCCAAGGAGCCAATACGTCGTAATTGATTCCGGCAGCGACATCGTTTGGGTGCAATGCCAGCCCTGCAGCGAGTGCTACCAACAGTCCGACCCGGTGTTCGACCCGGCCGGCTCCGCCTCCTACGCCGGAGTCTCCTGTGACTCTTCGGTGTGCGACCGCCTCGACAACGCCGGCTGCAACAACGGCCGGTGCCGGTACGAGGTATCGTACGGCGACGGGTCCTACACCCGCGGAACTCTCGCTCTCGAAACCCTAACTTTCGGCCGCGTCGTAATCCGAAACATCGCGATCGGTTGCGGCCATATGAACCGAGGAATGTTCGTCGGAGCCGCCGGATTGCTTGGACTTGGCGGCGGCGCCATGTCGTTCGTAGGCCAGCTCGGCGGCCAAACCGGCGGTGCGTTCAGCTACTGCTTGGTGAGTCGAGGCATCGAGTCCACCGGAACGCTGGAGTTCGGCCGCGGCGCCATGCCGGTCGGCGGCGCGTGGGTTCCCCTAATCCGAAACCCACGCGCTCCCAGTTTCTACTACGTCGGGCTTTCAGGTCTCGGAGTCGGAGGGATCCGAGTTCCAATACCCGAACAGATCTTCGAACTCAAAGAACTAGGATACGGCGGCGTCGTAATGGACACCGGCACCGCCGTAACGAGGCTACCGGCGCCCGCATACGAAGCATTTCGAGACACCTTCATCGAACAAACGGCGAACCTGCCTCGATCAGGCGGAGTATCGATCTTCGACACATGCTATGACCTAAACGGATTCGTATCGGTGAGGGTACCGACGGTGTCGTTCTACTTCTCCGGCGGGCCGATACTGACTTTGCCGGCGAGGAACTTTCTGATTCCGGTGGACGGGGAGGGAACATTTTGCTTTGCATTTGCAGCATCGGCGTCGGAATTGTCGATCATAGGGAACATCCAACAAGAAGGGATTCAAATCTCCGTTGATGGAGCAAATGGGTTTGTGGGATTTGGTCCAAGTATTTGTTAATTCATCAAAACTCAAAGGGCAAATCTGTCATTTTCAGTAGTCTTACATAGTATAAATTCTCTTTGCCTACTTATTTTATTATAATCTATTTTTTATTAGGTTAAATTATAGCTTTAATTTTAAAGTAAATCCCTCACTTTTAAAAAGTGTATAATGTATCATT

Coding sequence (CDS)

ATGCTACTCTTCCTCCTCCTTCTTCTTCCTCTTTTAGCAACCGCCGTCGACCCGGTCGCCACCGGCCTCGCCGCTACTTACCCGGCCACCCAACTCCTAAACGTCAAAGACACAATCAAAGAAACAGAAACCAAGCCCTCTAGACTACCACAAGATCTCGACCTCCCTGAAAACAACCTTCCTCTTGACAACAGCAGCAGTCAGAGTCAATGGAAGCTCAAGCTCTTCCACAGAGATAAGCTACCCCTCAACTTCGACCCCGACCACCGTCGCCGTTTCAAGGAGCGTATCGGAAGAGACGTCGAGAGGGTCTCCTCTTTGCTCCGCCAACTCTCCAATGGCAGCGACGAGCAGGTGACGGACTTTGGGTCGGACGTGGTCTCCGGCACGGAGCAGGGTAGCGGAGAGTACTTTGTGAGGATCGGCGTCGGCAGCCCGCCAAGGAGCCAATACGTCGTAATTGATTCCGGCAGCGACATCGTTTGGGTGCAATGCCAGCCCTGCAGCGAGTGCTACCAACAGTCCGACCCGGTGTTCGACCCGGCCGGCTCCGCCTCCTACGCCGGAGTCTCCTGTGACTCTTCGGTGTGCGACCGCCTCGACAACGCCGGCTGCAACAACGGCCGGTGCCGGTACGAGGTATCGTACGGCGACGGGTCCTACACCCGCGGAACTCTCGCTCTCGAAACCCTAACTTTCGGCCGCGTCGTAATCCGAAACATCGCGATCGGTTGCGGCCATATGAACCGAGGAATGTTCGTCGGAGCCGCCGGATTGCTTGGACTTGGCGGCGGCGCCATGTCGTTCGTAGGCCAGCTCGGCGGCCAAACCGGCGGTGCGTTCAGCTACTGCTTGGTGAGTCGAGGCATCGAGTCCACCGGAACGCTGGAGTTCGGCCGCGGCGCCATGCCGGTCGGCGGCGCGTGGGTTCCCCTAATCCGAAACCCACGCGCTCCCAGTTTCTACTACGTCGGGCTTTCAGGTCTCGGAGTCGGAGGGATCCGAGTTCCAATACCCGAACAGATCTTCGAACTCAAAGAACTAGGATACGGCGGCGTCGTAATGGACACCGGCACCGCCGTAACGAGGCTACCGGCGCCCGCATACGAAGCATTTCGAGACACCTTCATCGAACAAACGGCGAACCTGCCTCGATCAGGCGGAGTATCGATCTTCGACACATGCTATGACCTAAACGGATTCGTATCGGTGAGGGTACCGACGGTGTCGTTCTACTTCTCCGGCGGGCCGATACTGACTTTGCCGGCGAGGAACTTTCTGATTCCGGTGGACGGGGAGGGAACATTTTGCTTTGCATTTGCAGCATCGGCGTCGGAATTGTCGATCATAGGGAACATCCAACAAGAAGGGATTCAAATCTCCGTTGATGGAGCAAATGGGTTTGTGGGATTTGGTCCAAGTATTTGTTAA

Protein sequence

MLLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENNLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC

Homology

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BLAST of Lcy09g013340 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 4.6e-181
Identity = 304/428 (71.03%), Postives = 358/428 (83.64%), Query Frame = 0


Query: 55  LPENNLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQL--- 114
           LP+ N    +  S S++ L+L HRD+ P     +H  R   R+ RD +RVS++LR++   
Sbjct: 43  LPDFNNTHFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGK 102

Query: 115 ---SNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPC 174
              S+ S  +V DFGSD+VSG +QGSGEYFVRIGVGSPPR QY+VIDSGSD+VWVQCQPC
Sbjct: 103 VIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC 162

Query: 175 SECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLAL 234
             CY+QSDPVFDPA S SY GVSC SSVCDR++N+GC++G CRYEV YGDGSYT+GTLAL
Sbjct: 163 KLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLAL 222

Query: 235 ETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSR 294
           ETLTF + V+RN+A+GCGH NRGMF+GAAGLLG+GGG+MSFVGQL GQTGGAF YCLVSR
Sbjct: 223 ETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSR 282

Query: 295 GIESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKEL 354
           G +STG+L FGR A+PVG +WVPL+RNPRAPSFYYVGL GLGVGG+R+P+P+ +F+L E 
Sbjct: 283 GTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTET 342

Query: 355 GYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPT 414
           G GGVVMDTGTAVTRLP  AY AFRD F  QTANLPR+ GVSIFDTCYDL+GFVSVRVPT
Sbjct: 343 GDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPT 402

Query: 415 VSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGF 474
           VSFYF+ GP+LTLPARNFL+PVD  GT+CFAFAAS + LSIIGNIQQEGIQ+S DGANGF
Sbjct: 403 VSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGF 462

Query: 475 VGFGPSIC 477
           VGFGP++C
Sbjct: 463 VGFGPNVC 470

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BLAST of Lcy09g013340 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.5e-110
Identity = 216/496 (43.55%), Postives = 308/496 (62.10%), Query Frame = 0


Query: 3   LFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETET----KPSRLPQDLDLPEN 62
           L  ++ L L  T  D  +  L +T P T +L+V  ++++T+T     P+R       PE+
Sbjct: 9   LLAVVTLSLFLTTTDASSRSL-STPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPES 68

Query: 63  NLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLS---NGS 122
                  +S S   L+L  RD    +   D++     R+ RD  RV+ ++ ++     G 
Sbjct: 69  LSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGV 128

Query: 123 DE-------------QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVW 182
           D              Q  D  + VVSG  QGSGEYF RIGVG+P +  Y+V+D+GSD+ W
Sbjct: 129 DRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNW 188

Query: 183 VQCQPCSECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYT 242
           +QC+PC++CYQQSDPVF+P  S++Y  ++C +  C  L+ + C + +C Y+VSYGDGS+T
Sbjct: 189 IQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFT 248

Query: 243 RGTLALETLTFGRV-VIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAF 302
            G LA +T+TFG    I N+A+GCGH N G+F GAAGLLGLGGG +S   Q+      +F
Sbjct: 249 VGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---KATSF 308

Query: 303 SYCLVSRGIESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQ 362
           SYCLV R    + +L+F    +  G A  PL+RN +  +FYYVGLSG  VGG +V +P+ 
Sbjct: 309 SYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDA 368

Query: 363 IFELKELGYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLPR-SGGVSIFDTCYDLNG 422
           IF++   G GGV++D GTAVTRL   AY + RD F++ T NL + S  +S+FDTCYD + 
Sbjct: 369 IFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSS 428

Query: 423 FVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQI 477
             +V+VPTV+F+F+GG  L LPA+N+LIPVD  GTFCFAFA ++S LSIIGN+QQ+G +I
Sbjct: 429 LSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRI 488

[Close]

BLAST of Lcy09g013340 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.4e-108
Identity = 213/423 (50.35%), Postives = 268/423 (63.36%), Query Frame = 0


Query: 64  NSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQVT--- 123
           +S S S   L L H D L  N  PD    F  R+ RD  RV S+    +      VT   
Sbjct: 65  DSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP 124

Query: 124 ---DFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 183
               F S VVSG  QGSGEYF R+GVG+P R  Y+V+D+GSDIVW+QC PC  CY QSDP
Sbjct: 125 RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP 184

Query: 184 VFDPAGSASYAGVSCDSSVCDRLDNAGCNNGR--CRYEVSYGDGSYTRGTLALETLTFGR 243
           +FDP  S +YA + C S  C RLD+AGCN  R  C Y+VSYGDGS+T G  + ETLTF R
Sbjct: 185 IFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 244

Query: 244 VVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIES-TG 303
             ++ +A+GCGH N G+FVGAAGLLGLG G +SF GQ G +    FSYCLV R   S   
Sbjct: 245 NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPS 304

Query: 304 TLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP-IPEQIFELKELGYGGV 363
           ++ FG  A+     + PL+ NP+  +FYYVGL G+ VGG RVP +   +F+L ++G GGV
Sbjct: 305 SVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 364

Query: 364 VMDTGTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYF 423
           ++D+GT+VTRL  PAY A RD F      L R+   S+FDTC+DL+    V+VPTV  +F
Sbjct: 365 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF 424

Query: 424 SGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGP 477
            G  + +LPA N+LIPVD  G FCFAFA +   LSIIGNIQQ+G ++  D A+  VGF P
Sbjct: 425 RGADV-SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAP 484

[Close]

BLAST of Lcy09g013340 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.4e-67
Identity = 162/458 (35.37%), Postives = 240/458 (52.40%), Query Frame = 0


Query: 40  KETETKPSRLPQDLDLPENNLPLDNSSSQSQWKLKLFHRDKLPLNFD------PDHRRRF 99
           +ET++   ++   L    ++  L   +S ++  L + HR       +      PDH    
Sbjct: 29  RETDSHTIQVSSLLPSSSSSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDH---- 88

Query: 100 KERIGRDVERVSSLLRQLS------NGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPP 159
            E +  D  RV+S+  +LS      + S+ + TD  +    G+  GSG Y V +G+G+P 
Sbjct: 89  VEILRLDQARVNSIHSKLSKKLATDHVSESKSTDLPAK--DGSTLGSGNYIVTVGLGTPK 148

Query: 160 RSQYVVIDSGSDIVWVQCQPC-SECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNA--- 219
               ++ D+GSD+ W QCQPC   CY Q +P+F+P+ S SY  VSC S+ C  L +A   
Sbjct: 149 NDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGN 208

Query: 220 --GCNNGRCRYEVSYGDGSYTRGTLALETLTF-GRVVIRNIAIGCGHMNRGMFVGAAGLL 279
              C+   C Y + YGD S++ G LA E  T     V   +  GCG  N+G+F G AGLL
Sbjct: 209 AGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLL 268

Query: 280 GLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFGRGAMPVGGAWVPLIRNPRAPS 339
           GLG   +SF  Q        FSYCL S     TG L FG   +     + P+       S
Sbjct: 269 GLGRDKLSFPSQTATAYNKIFSYCLPS-SASYTGHLTFGSAGISRSVKFTPISTITDGTS 328

Query: 340 FYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQT 399
           FY + +  + VGG ++PIP  +F        G ++D+GT +TRLP  AY A R +F  + 
Sbjct: 329 FYGLNIVAITVGGQKLPIPSTVFSTP-----GALIDSGTVITRLPPKAYAALRSSFKAKM 388

Query: 400 ANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAF 459
           +  P + GVSI DTC+DL+GF +V +P V+F FSGG ++ L ++  +  V      C AF
Sbjct: 389 SKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKG-IFYVFKISQVCLAF 448

Query: 460 AASA--SELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           A ++  S  +I GN+QQ+ +++  DGA G VGF P+ C
Sbjct: 449 AGNSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

[Close]

BLAST of Lcy09g013340 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.7e-66
Identity = 146/384 (38.02%), Postives = 215/384 (55.99%), Query Frame = 0


Query: 99  RDVERVSSLLRQLSNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGS 158
           R +ER S  L++L     E + +  S V +    G GEY + + +G+P +    ++D+GS
Sbjct: 62  RAIERGSRRLQRL-----EAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGS 121

Query: 159 DIVWVQCQPCSECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGD 218
           D++W QCQPC++C+ QS P+F+P GS+S++ + C S +C  L +  C+N  C+Y   YGD
Sbjct: 122 DLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGD 181

Query: 219 GSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVG-AAGLLGLGGGAMSFVGQLGGQT 278
           GS T+G++  ETLTFG V I NI  GCG  N+G   G  AGL+G+G G +S   QL    
Sbjct: 182 GSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVT- 241

Query: 279 GGAFSYCLVSRGIESTGTLEFG--RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIR 338
              FSYC+   G  +   L  G    ++  G     LI++ + P+FYY+ L+GL VG  R
Sbjct: 242 --KFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTR 301

Query: 339 VPIPEQIFEL-KELGYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLP-RSGGVSIFD 398
           +PI    F L    G GG+++D+GT +T     AY++ R  FI Q  NLP  +G  S FD
Sbjct: 302 LPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNGSSSGFD 361

Query: 399 TCYDLNGFVS-VRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGN 458
            C+      S +++PT   +F GG  L LP+ N+ I     G  C A  +S+  +SI GN
Sbjct: 362 LCFQTPSDPSNLQIPTFVMHFDGGD-LELPSENYFIS-PSNGLICLAMGSSSQGMSIFGN 421

Query: 459 IQQEGIQISVDGANGFVGFGPSIC 477
           IQQ+ + +  D  N  V F  + C
Sbjct: 422 IQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Lcy09g013340 vs. ExPASy TrEMBL
Match: A0A5A7VGC3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00380 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.0e-247
Identity = 437/477 (91.61%), Postives = 452/477 (94.76%), Query Frame = 0


Query: 2   LLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENN-- 61
           +L LLLLLPLLATAV  VATG AATYP TQLLNVKDTIKETET PSRLPQDLDL EN   
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPVTQLLNVKDTIKETETTPSRLPQDLDLHENYPL 60

Query: 62  LPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQV 121
             LDN+SSQSQWKLKLFHRDKLPLNFD +H RRFKERI RD +RVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 122 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 181
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 182 DPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 241
           DPAGSA+YAG+SCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 242 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFG 301
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 302 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGT 361
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 362 AVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPIL 421
           AVTRLPAPAYEAFRDTFI QTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 422 TLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           TLPARNFLIPVDGEGTFCFAFAASAS LSIIGNIQQEGIQIS+DG+NGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lcy09g013340 vs. ExPASy TrEMBL
Match: A0A1S3CNF7 (protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo OX=3656 GN=LOC103502903 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 437/477 (91.61%), Postives = 453/477 (94.97%), Query Frame = 0


Query: 2   LLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENN-- 61
           +L LLLLLPLLATAV  VATG AATYPATQLLNVKDTIKETET PSRLPQDL+L EN   
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPATQLLNVKDTIKETETTPSRLPQDLNLHENYPL 60

Query: 62  LPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQV 121
             LDN+SSQSQWKLKLFHRDKLPLNFD +H RRFKERI RD +RVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 122 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 181
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 182 DPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 241
           DPAGSA+YAG+SCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 242 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFG 301
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 302 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGT 361
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 362 AVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPIL 421
           AVTRLPAPAYEAFRDTFI QTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 422 TLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           TLPARNFLIPVDGEGTFCFAFAASAS LSIIGNIQQEGIQIS+DG+NGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lcy09g013340 vs. ExPASy TrEMBL
Match: A0A6J1E0R8 (protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucurbita moschata OX=3662 GN=LOC111429766 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.7e-247
Identity = 438/476 (92.02%), Postives = 452/476 (94.96%), Query Frame = 0


Query: 2   LLFLLLLLPLLA-TAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENNL 61
           LL LLLL PLL   AVD VA+ LAA+YPATQLL+VKDTIKETE KPSRLPQDL+L EN  
Sbjct: 5   LLLLLLLPPLLTRAAVDTVASSLAASYPATQLLHVKDTIKETEIKPSRLPQDLELHENYP 64

Query: 62  PLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQVT 121
           P+DN S+Q+QWKL+LFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLR+LSNGSDEQVT
Sbjct: 65  PIDN-STQNQWKLELFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRRLSNGSDEQVT 124

Query: 122 DFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFD 181
           DFGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFD
Sbjct: 125 DFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFD 184

Query: 182 PAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRN 241
           PA SASYAG+SCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV+IRN
Sbjct: 185 PASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRVLIRN 244

Query: 242 IAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFGR 301
           IAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEFGR
Sbjct: 245 IAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGR 304

Query: 302 GAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGTA 361
            AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTGTA
Sbjct: 305 SAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTA 364

Query: 362 VTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILT 421
           VTRLP PAYEAFRDTFI QTANLPRS  VSIFDTCYDLNGFVSVRVPTVSFYFSGGPILT
Sbjct: 365 VTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILT 424

Query: 422 LPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           LPARNFLIPVDGEGTFCFAFAAS S LSIIGNIQQEGIQISVDGANGFVGFGPSIC
Sbjct: 425 LPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 479

[Close]

BLAST of Lcy09g013340 vs. ExPASy TrEMBL
Match: A0A6J1JNZ5 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487042 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 439/479 (91.65%), Postives = 453/479 (94.57%), Query Frame = 0


Query: 1   MLLFLLLLL--PLLA-TAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPE 60
           ML FLLLLL  PLL   A+D VATGLAA YPATQLL+VKDTIKETE KPSRLPQDL+L E
Sbjct: 1   MLPFLLLLLLPPLLTRAAIDTVATGLAANYPATQLLHVKDTIKETEIKPSRLPQDLELYE 60

Query: 61  NNLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDE 120
           N  P+DN S+Q+QWKL+LFHRDKLPLNFDPDH RRFKERIGRDVERVSSLLR+LSNGSDE
Sbjct: 61  NYPPIDN-STQNQWKLELFHRDKLPLNFDPDHCRRFKERIGRDVERVSSLLRRLSNGSDE 120

Query: 121 QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180
           QVT+FGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP
Sbjct: 121 QVTEFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180

Query: 181 VFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVV 240
           VFDPA SASYAG+SCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV+
Sbjct: 181 VFDPASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRVL 240

Query: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLE 300
           IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLE
Sbjct: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE 300

Query: 301 FGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDT 360
           FGR AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDT
Sbjct: 301 FGRSAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT 360

Query: 361 GTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGP 420
           GTAVTRLP PAYEAFRDTFI QTANLPRS  VSIFDTCYDLNGFVSVRVPTVSFYFSGGP
Sbjct: 361 GTAVTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGGP 420

Query: 421 ILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           ILTLPARNFLIPVDGEGTFCFAFAAS S LSIIGNIQQEGIQISVDGANGFVGFGPSIC
Sbjct: 421 ILTLPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 478

[Close]

BLAST of Lcy09g013340 vs. ExPASy TrEMBL
Match: A0A6J1CII3 (protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Momordica charantia OX=3673 GN=LOC111011806 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.3e-236
Identity = 417/475 (87.79%), Postives = 437/475 (92.00%), Query Frame = 0


Query: 2   LLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENNLP 61
           +L  LL LPLL   V        A YP TQLLNVKD  KETE++P+RLP+DL L E N P
Sbjct: 5   MLPFLLFLPLLTATV-----AATAAYPVTQLLNVKDITKETESEPTRLPEDLPLHE-NYP 64

Query: 62  LDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQVTD 121
           + N+++ +QWKLKLFHRD LPLNFDPDHR RFKER+ RD++RVSSLLRQLSN S+EQVTD
Sbjct: 65  IHNTTTHTQWKLKLFHRDNLPLNFDPDHRCRFKERMERDLKRVSSLLRQLSNDSNEQVTD 124

Query: 122 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDP 181
           FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCS+CYQQSDPVFDP
Sbjct: 125 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSKCYQQSDPVFDP 184

Query: 182 AGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNI 241
           AGSASYAGVSCDSSVCDRLDNAGCN+GRCRYEV+YGDGSYT GTLALETLTFGRV+IRNI
Sbjct: 185 AGSASYAGVSCDSSVCDRLDNAGCNDGRCRYEVAYGDGSYTSGTLALETLTFGRVMIRNI 244

Query: 242 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFGRG 301
           AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTG+LEFGRG
Sbjct: 245 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGSLEFGRG 304

Query: 302 AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGTAV 361
           AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGG RVPIPEQIFEL  LGYGGVVMDTGTAV
Sbjct: 305 AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGTRVPIPEQIFELTNLGYGGVVMDTGTAV 364

Query: 362 TRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTL 421
           TRLPAPAYEAFRDTFI  TANLPRS GVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTL
Sbjct: 365 TRLPAPAYEAFRDTFIGLTANLPRSAGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTL 424

Query: 422 PARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           PARNFLIPVDGEGTFCFAFAAS S LSIIGNIQQEGIQIS+DGANGFVGFGPSIC
Sbjct: 425 PARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISIDGANGFVGFGPSIC 473

[Close]

BLAST of Lcy09g013340 vs. NCBI nr
Match: XP_038875928.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Benincasa hispida])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 437/475 (92.00%), Postives = 451/475 (94.95%), Query Frame = 0


Query: 2   LLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENNLP 61
           +L  LLLLPLLATA+  V+T  AATYPATQLLNVKDTIKETET+PSRLPQDLDL EN  P
Sbjct: 1   MLPFLLLLPLLATAISLVSTSPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60

Query: 62  LDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQVTD 121
            DNSSSQSQWKLKLFHRDKLP NF+ DH RRFKERI RD  RVSSLLR LSNGSDEQVTD
Sbjct: 61  FDNSSSQSQWKLKLFHRDKLPFNFNTDHPRRFKERIERDSIRVSSLLRLLSNGSDEQVTD 120

Query: 122 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDP 181
           FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPC+ECYQQSDPVFDP
Sbjct: 121 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCNECYQQSDPVFDP 180

Query: 182 AGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNI 241
           AGSASYAG+SCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGRV+IRNI
Sbjct: 181 AGSASYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRVIIRNI 240

Query: 242 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFGRG 301
           AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEFGRG
Sbjct: 241 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRG 300

Query: 302 AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGTAV 361
           AMPVGGAWVPLIRNPRAPSFYYV LSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTGTAV
Sbjct: 301 AMPVGGAWVPLIRNPRAPSFYYVRLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAV 360

Query: 362 TRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTL 421
           TRLPAPAYEA RDTFI QTANLPRSG VSIFDTCY+LNGF+SVRVPTVSFYFSGGPILTL
Sbjct: 361 TRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFISVRVPTVSFYFSGGPILTL 420

Query: 422 PARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           PARNFLIPVDGEGTFCFAFAASAS LSIIGNIQQEGIQIS+DG+NGFVGFGPSIC
Sbjct: 421 PARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 475

[Close]

BLAST of Lcy09g013340 vs. NCBI nr
Match: XP_023514620.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.9 bits (2236), Expect = 1.6e-247
Identity = 439/478 (91.84%), Postives = 452/478 (94.56%), Query Frame = 0


Query: 1   MLLFLLLLLPLLAT--AVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPEN 60
           ML F LLLLP L T  AVD VATGL A YPATQLL+VKDTIKETE KPSRLPQDL+L EN
Sbjct: 1   MLPFFLLLLPPLLTRAAVDTVATGLVANYPATQLLHVKDTIKETEIKPSRLPQDLELYEN 60

Query: 61  NLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQ 120
             P+DN S+Q+QWKL+LFHRDKLPLNFDPDHRRRFKERIGRDV+RVSSLLR+LSNGSDEQ
Sbjct: 61  YPPIDN-STQNQWKLELFHRDKLPLNFDPDHRRRFKERIGRDVQRVSSLLRRLSNGSDEQ 120

Query: 121 VTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPV 180
           VTDFGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPV
Sbjct: 121 VTDFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPV 180

Query: 181 FDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVI 240
           FDPA SASYAG+SCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV+I
Sbjct: 181 FDPASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRVLI 240

Query: 241 RNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEF 300
           RNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEF
Sbjct: 241 RNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEF 300

Query: 301 GRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTG 360
           GR AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTG
Sbjct: 301 GRSAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTG 360

Query: 361 TAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPI 420
           TAVTRLP PAYEAFRDTFI QTANLPRS  VSIFDTCYDLNGFVSVRVPTVSFYFSGGPI
Sbjct: 361 TAVTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGGPI 420

Query: 421 LTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           LTLPARNFLIPVDGEGTFCFAFAAS S LSIIGNIQQEGIQISVDGANGFVGFGPSIC
Sbjct: 421 LTLPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 477

[Close]

BLAST of Lcy09g013340 vs. NCBI nr
Match: KAA0066127.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo var. makuwa] >TYK15170.1 protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo var. makuwa])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 437/477 (91.61%), Postives = 452/477 (94.76%), Query Frame = 0


Query: 2   LLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENN-- 61
           +L LLLLLPLLATAV  VATG AATYP TQLLNVKDTIKETET PSRLPQDLDL EN   
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPVTQLLNVKDTIKETETTPSRLPQDLDLHENYPL 60

Query: 62  LPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQV 121
             LDN+SSQSQWKLKLFHRDKLPLNFD +H RRFKERI RD +RVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 122 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 181
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 182 DPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 241
           DPAGSA+YAG+SCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 242 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFG 301
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 302 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGT 361
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 362 AVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPIL 421
           AVTRLPAPAYEAFRDTFI QTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 422 TLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           TLPARNFLIPVDGEGTFCFAFAASAS LSIIGNIQQEGIQIS+DG+NGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lcy09g013340 vs. NCBI nr
Match: KAG7032671.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 441/479 (92.07%), Postives = 454/479 (94.78%), Query Frame = 0


Query: 1   MLLFLLLLL--PLLA-TAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPE 60
           ML FLLLLL  PLL   AVD VA+GLAA+YPATQLL+VKDTIKETE KPSRLPQDL+L E
Sbjct: 1   MLPFLLLLLLPPLLTRAAVDTVASGLAASYPATQLLHVKDTIKETEIKPSRLPQDLELHE 60

Query: 61  NNLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDE 120
           N  P+DN S+Q QWKL+LFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLR+LSNGSDE
Sbjct: 61  NYPPIDN-STQKQWKLELFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRRLSNGSDE 120

Query: 121 QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180
           QVTDFGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP
Sbjct: 121 QVTDFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180

Query: 181 VFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVV 240
           VFDPA SASYAG+SCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV+
Sbjct: 181 VFDPASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRVL 240

Query: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLE 300
           IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLE
Sbjct: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE 300

Query: 301 FGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDT 360
           FGR AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDT
Sbjct: 301 FGRSAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT 360

Query: 361 GTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGP 420
           GTAVTRLP PAYEAFRDTFI QTANLPRS  VSIFDTCYDLNGFVSVRVPTVSFYFSGGP
Sbjct: 361 GTAVTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGGP 420

Query: 421 ILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           ILTLPARNFLIPVDGEGTFCFAFAAS S LSIIGNIQQEGIQISVDGANGFVGFGPSIC
Sbjct: 421 ILTLPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 478

[Close]

BLAST of Lcy09g013340 vs. NCBI nr
Match: XP_008465249.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo])

HSP 1 Score: 865.1 bits (2234), Expect = 2.7e-247
Identity = 437/477 (91.61%), Postives = 453/477 (94.97%), Query Frame = 0


Query: 2   LLFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETETKPSRLPQDLDLPENN-- 61
           +L LLLLLPLLATAV  VATG AATYPATQLLNVKDTIKETET PSRLPQDL+L EN   
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPATQLLNVKDTIKETETTPSRLPQDLNLHENYPL 60

Query: 62  LPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQV 121
             LDN+SSQSQWKLKLFHRDKLPLNFD +H RRFKERI RD +RVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 122 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 181
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 182 DPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 241
           DPAGSA+YAG+SCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 242 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFG 301
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRG ESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 302 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGT 361
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFEL +LGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 362 AVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPIL 421
           AVTRLPAPAYEAFRDTFI QTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 422 TLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           TLPARNFLIPVDGEGTFCFAFAASAS LSIIGNIQQEGIQIS+DG+NGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lcy09g013340 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 635.6 bits (1638), Expect = 3.3e-182
Identity = 304/428 (71.03%), Postives = 358/428 (83.64%), Query Frame = 0


Query: 55  LPENNLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQL--- 114
           LP+ N    +  S S++ L+L HRD+ P     +H  R   R+ RD +RVS++LR++   
Sbjct: 43  LPDFNNTHFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGK 102

Query: 115 ---SNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPC 174
              S+ S  +V DFGSD+VSG +QGSGEYFVRIGVGSPPR QY+VIDSGSD+VWVQCQPC
Sbjct: 103 VIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC 162

Query: 175 SECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLAL 234
             CY+QSDPVFDPA S SY GVSC SSVCDR++N+GC++G CRYEV YGDGSYT+GTLAL
Sbjct: 163 KLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLAL 222

Query: 235 ETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSR 294
           ETLTF + V+RN+A+GCGH NRGMF+GAAGLLG+GGG+MSFVGQL GQTGGAF YCLVSR
Sbjct: 223 ETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSR 282

Query: 295 GIESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKEL 354
           G +STG+L FGR A+PVG +WVPL+RNPRAPSFYYVGL GLGVGG+R+P+P+ +F+L E 
Sbjct: 283 GTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTET 342

Query: 355 GYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPT 414
           G GGVVMDTGTAVTRLP  AY AFRD F  QTANLPR+ GVSIFDTCYDL+GFVSVRVPT
Sbjct: 343 GDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPT 402

Query: 415 VSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGF 474
           VSFYF+ GP+LTLPARNFL+PVD  GT+CFAFAAS + LSIIGNIQQEGIQ+S DGANGF
Sbjct: 403 VSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGF 462

Query: 475 VGFGPSIC 477
           VGFGP++C
Sbjct: 463 VGFGPNVC 470

[Close]

BLAST of Lcy09g013340 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 409.1 bits (1050), Expect = 5.0e-114
Identity = 207/463 (44.71%), Postives = 296/463 (63.93%), Query Frame = 0


Query: 30  TQLLNVKDTIKETE-TKPSRLPQDLDLPENNLPLDNSSSQSQWKLKLFHRDKLPLNFDPD 89
           T +LNV D+I  T+ T   RL Q  +           S+ S + L+L  R  +      D
Sbjct: 33  TSILNVADSIHRTKYTSSFRLNQQEE--------QTHSASSSFSLQLHSRVSVRGTEHSD 92

Query: 90  HRRRFKERIGRDVERVSSL---------------LRQLSNGSDEQVTDFGSDVVSGTEQG 149
           ++     R+ RD  RV SL               L+ +S     +  D  + ++SGT QG
Sbjct: 93  YKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQG 152

Query: 150 SGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDPAGSASYAGVSCD 209
           SGEYF R+G+G P R  Y+V+D+GSD+ W+QC PC++CY Q++P+F+P+ S+SY  +SCD
Sbjct: 153 SGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCD 212

Query: 210 SSVCDRLDNAGCNNGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMF 269
           +  C+ L+ + C N  C YEVSYGDGSYT G  A ETLT G  +++N+A+GCGH N G+F
Sbjct: 213 TPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLF 272

Query: 270 VGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIESTGTLEFGRGAMPVGGAWVPLI 329
           VGAAGLLGLGGG ++   QL      +FSYCLV R  +S  T++FG    P      PL+
Sbjct: 273 VGAAGLLGLGGGLLALPSQLNTT---SFSYCLVDRDSDSASTVDFGTSLSP-DAVVAPLL 332

Query: 330 RNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELKELGYGGVVMDTGTAVTRLPAPAYEAFR 389
           RN +  +FYY+GL+G+ VGG  + IP+  FE+ E G GG+++D+GTAVTRL    Y + R
Sbjct: 333 RNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLR 392

Query: 390 DTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGE 449
           D+F++ T +L ++ GV++FDTCY+L+   +V VPTV+F+F GG +L LPA+N++IPVD  
Sbjct: 393 DSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSV 452

Query: 450 GTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGPSIC 477
           GTFC AFA +AS L+IIGN+QQ+G +++ D AN  +GF  + C
Sbjct: 453 GTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Lcy09g013340 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-111
Identity = 216/496 (43.55%), Postives = 308/496 (62.10%), Query Frame = 0


Query: 3   LFLLLLLPLLATAVDPVATGLAATYPATQLLNVKDTIKETET----KPSRLPQDLDLPEN 62
           L  ++ L L  T  D  +  L +T P T +L+V  ++++T+T     P+R       PE+
Sbjct: 9   LLAVVTLSLFLTTTDASSRSL-STPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPES 68

Query: 63  NLPLDNSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLS---NGS 122
                  +S S   L+L  RD    +   D++     R+ RD  RV+ ++ ++     G 
Sbjct: 69  LSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGV 128

Query: 123 DE-------------QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVW 182
           D              Q  D  + VVSG  QGSGEYF RIGVG+P +  Y+V+D+GSD+ W
Sbjct: 129 DRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNW 188

Query: 183 VQCQPCSECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAGCNNGRCRYEVSYGDGSYT 242
           +QC+PC++CYQQSDPVF+P  S++Y  ++C +  C  L+ + C + +C Y+VSYGDGS+T
Sbjct: 189 IQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFT 248

Query: 243 RGTLALETLTFGRV-VIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAF 302
            G LA +T+TFG    I N+A+GCGH N G+F GAAGLLGLGGG +S   Q+      +F
Sbjct: 249 VGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---KATSF 308

Query: 303 SYCLVSRGIESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQ 362
           SYCLV R    + +L+F    +  G A  PL+RN +  +FYYVGLSG  VGG +V +P+ 
Sbjct: 309 SYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDA 368

Query: 363 IFELKELGYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLPR-SGGVSIFDTCYDLNG 422
           IF++   G GGV++D GTAVTRL   AY + RD F++ T NL + S  +S+FDTCYD + 
Sbjct: 369 IFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSS 428

Query: 423 FVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQI 477
             +V+VPTV+F+F+GG  L LPA+N+LIPVD  GTFCFAFA ++S LSIIGN+QQ+G +I
Sbjct: 429 LSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRI 488

[Close]

BLAST of Lcy09g013340 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 9.7e-110
Identity = 213/423 (50.35%), Postives = 268/423 (63.36%), Query Frame = 0


Query: 64  NSSSQSQWKLKLFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRQLSNGSDEQVT--- 123
           +S S S   L L H D L  N  PD    F  R+ RD  RV S+    +      VT   
Sbjct: 65  DSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP 124

Query: 124 ---DFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 183
               F S VVSG  QGSGEYF R+GVG+P R  Y+V+D+GSDIVW+QC PC  CY QSDP
Sbjct: 125 RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP 184

Query: 184 VFDPAGSASYAGVSCDSSVCDRLDNAGCNNGR--CRYEVSYGDGSYTRGTLALETLTFGR 243
           +FDP  S +YA + C S  C RLD+AGCN  R  C Y+VSYGDGS+T G  + ETLTF R
Sbjct: 185 IFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 244

Query: 244 VVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGIES-TG 303
             ++ +A+GCGH N G+FVGAAGLLGLG G +SF GQ G +    FSYCLV R   S   
Sbjct: 245 NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPS 304

Query: 304 TLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP-IPEQIFELKELGYGGV 363
           ++ FG  A+     + PL+ NP+  +FYYVGL G+ VGG RVP +   +F+L ++G GGV
Sbjct: 305 SVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 364

Query: 364 VMDTGTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIFDTCYDLNGFVSVRVPTVSFYF 423
           ++D+GT+VTRL  PAY A RD F      L R+   S+FDTC+DL+    V+VPTV  +F
Sbjct: 365 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF 424

Query: 424 SGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGNIQQEGIQISVDGANGFVGFGP 477
            G  + +LPA N+LIPVD  G FCFAFA +   LSIIGNIQQ+G ++  D A+  VGF P
Sbjct: 425 RGADV-SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAP 484

[Close]

BLAST of Lcy09g013340 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-101
Identity = 203/444 (45.72%), Postives = 267/444 (60.14%), Query Frame = 0


Query: 53  LDLPENNLPLDNSSSQSQWKLK--LFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLL-- 112
           L  PE+    D S S+S   L   L H D L    D      F  R+ RD  RV S+   
Sbjct: 41  LSWPESESLTDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSL 100

Query: 113 ------RQLSNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVW 172
                 R  +  +      F   V+SG  QGSGEYF+R+GVG+P  + Y+V+D+GSD+VW
Sbjct: 101 AAVSTGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVW 160

Query: 173 VQCQPCSECYQQSDPVFDPAGSASYAGVSCDSSVCDRLDNAG-CNNGR---CRYEVSYGD 232
           +QC PC  CY Q+D +FDP  S ++A V C S +C RLD++  C   R   C Y+VSYGD
Sbjct: 161 LQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGD 220

Query: 233 GSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTG 292
           GS+T G  + ETLTF    + ++ +GCGH N G+FVGAAGLLGLG G +SF  Q   +  
Sbjct: 221 GSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYN 280

Query: 293 GAFSYCLVSR-----GIESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGG 352
           G FSYCLV R       +   T+ FG  A+P    + PL+ NP+  +FYY+ L G+ VGG
Sbjct: 281 GKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGG 340

Query: 353 IRVP-IPEQIFELKELGYGGVVMDTGTAVTRLPAPAYEAFRDTFIEQTANLPRSGGVSIF 412
            RVP + E  F+L   G GGV++D+GT+VTRL  PAY A RD F      L R+   S+F
Sbjct: 341 SRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLF 400

Query: 413 DTCYDLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASELSIIGN 472
           DTC+DL+G  +V+VPTV F+F GG + +LPA N+LIPV+ EG FCFAFA +   LSIIGN
Sbjct: 401 DTCFDLSGMTTVKVPTVVFHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGN 460

Query: 473 IQQEGIQISVDGANGFVGFGPSIC 477
           IQQ+G +++ D     VGF    C
Sbjct: 461 IQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of Lcy09g013340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHE3	4.6e-181	71.03	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LS40	2.5e-110	43.55	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.4e-108	50.35	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q8S9J6	3.4e-67	35.37	Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
Q766C3	1.7e-66	38.02	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of Lcy09g013340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VGC3	1.0e-247	91.61	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CNF7	1.3e-247	91.61	protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo OX=3656 GN=LOC10350290...	[more]
A0A6J1E0R8	1.7e-247	92.02	protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JNZ5	1.5e-246	91.65	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1CII3	2.3e-236	87.79	protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of Lcy09g013340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875928.1	1.9e-248	92.00	protein ASPARTIC PROTEASE IN GUARD CELL 2 [Benincasa hispida]	[more]
XP_023514620.1	1.6e-247	91.84	protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo]	[more]
KAA0066127.1	2.1e-247	91.61	protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo var. makuwa] >TYK15170.1...	[more]
KAG7032671.1	2.1e-247	92.07	Protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucurbita argyrosperma subsp. argyros...	[more]
XP_008465249.1	2.7e-247	91.61	PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo]	[more]

BLAST of Lcy09g013340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20015.1	3.3e-182	71.03	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	5.0e-114	44.71	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	1.8e-111	43.55	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	9.7e-110	50.35	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.3e-101	45.72	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 321..472 e-value: 7.4E-30 score: 103.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 307..476 e-value: 1.1E-42 score: 147.7
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 115..299 e-value: 1.0E-50 score: 174.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 132..476
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 137..299 e-value: 9.6E-50 score: 169.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..66
None	No IPR available	PANTHER	PTHR13683:SF838	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2-LIKE	coord: 64..476
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 64..476
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 152..163
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 137..472 score: 44.117619

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g013340.1	Lcy09g013340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity