Lcy09g012980 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g012980
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionWAT1-related protein At3g02690, chloroplastic
LocationChr09: 14368364 .. 14372996 (-)
RNA-Seq ExpressionLcy09g012980
SyntenyLcy09g012980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCTCTTGGCCCTCCATCTCTTCCTGTTTAGCCACTGCCACACTCATTTCCTCTTCTTCTCCGTCCGACTCTCGCCCTCTTCTTCATTTCCGTTTCACCAGACGAATTCTCGCACCGGACATTTCCTCCGCCGCCCCAATCCTCCGGCGACGAGTGCCGTCTCATTTGGGTTTCAGAAGATACGGTGAGAATGACTGGTTTCGCTCCGATTATGTTGCAATTCCGGTGGTGAATTGTACCAGAAGTGGTGCGGATACCGAGTTGGATTTGTCTGAGTCCATCGAATGCGTGGGGACTGCTCAGGATGTGGAGTGCGTGGTTTCATCGAGTGATGAAGTTGAAGATCCTCTGTCCTCAATTGGGGAGTCACTGGAATTAGGGTTTTCTTCCGATGTTGTTGGTGGTGGTGGTGATGGCTCTGTGGCAGTCTTGGAGAAAGCCTGGGAGTTCGCCGTGTTGGTTTCGCCGTTTTTCTTTTGGGGTACGGCTATGGTGGCGATGAAAGAGGTGCTTCCAAGGTCTGGTCCCTTTTTCGTTTCCGCCTTTCGTCTTATACCTGCCGGTTTCCTTTTGATTGCCTTTGCTGCTTTCCGCGGTCGCCCCTTTCCCTCTGGTTTTTCTGCTTGGATTTCCATCCTTCTCTTTGCTCTTGTCGACGCTACATCGTTTCAGGGCTTTCTTGCTCAAGGCTTGCAGAGGACATCCGCAGGCTTGGGCAGTGTATGTTTCAGTTTTTGCATCTTTTGCTTCGTAACTTAGATGTCTCTTTTACTTGATTGTAGATTTCTGAAATTGGTAATTGATGTATAGTATATGTTGGCAGCGCTGGTACGCAAAAAAGAAAACAATAATGTATTCAAACTAAACCTAGAGAAAACAGCTAACCACAAGAAATTTATTTTGTGGAACTTTTTCAAATTTATTTTCTTTGCTTGCTTGATTACTAATGAGAGGGAGGATCCTTTTATATAGTATAACCCTTGTGACATTCAAGAAGACACATAGATAAACATTGGTATACAGTTGCATAGCATCATGTATCTTGTAAATTTGACCTCCATGTGCCTTAAAATGTATGATCCATACAACTTGAAATGTGTCGAGTAATTTCTAACATTCCCTTAAGCTGACTTCAATTGTTGACGAGTCTACCACATGTTATTTCTTTGAGGCCCATGAAATTTCACCAAAACAAATGTTAAACATATATCCAAAAGTACTTTTTTAATAGTCAACATTTCCTCTCCATTCCAACTGTCGTTGCTAAAACCCATTATATAGTAAGGTAGGTTGGTATGGTTGAAGAATAGAGATGAAAATTCAGCTCTTTCCTGTTGTATTTTGATTGAAAGGAAGAGGAAATTTGATTTGTGAATTGATATTGGATGAGGGTTGAATTTGAATATTGAGATGGGAGTGGCATACACGAGAACTTTTTCCGGAATTTATTCTATATAAATTTGGGGGTGAGGTTATTACCTGAGGGTGTGGATTGGCCACCAATTTTGGAGGAGAAGGTTTTGGATCATTCTCAAGATAGACTTCATGTGCATACTCCAAGTTGTTCTAGTTGTATTTTACGAGTCTCATCTCCCATGAAAGAATAATAAAGTCGAGAAATGGGGACAAACTTATAGAGATTGCTTTCCATCAATATCTATACGCGTCAGAGCCAACAATAATAGCATCCCACCCATATTCCTCTACTTGGAGTTTTCTTAATAAGAACTTTTGTTTTTATGATATGTTTCTTATTTGTGCCAAATGGGGGGAACGTACTTTAGCTTTCATAGGCATTATAATTATCTTTCTTTTCATTTTTCCAATGAAAAGAATTCAAAAAAAAAAAAAAAATTCATATTTGGCACTTTTTTCTCTACATCTTTGTACTCATTCAAATAGCTTATGGAAATTATATCCATAGCATTCACATTTTCTGTGCCTATGCCTTGATTTGCAGGTAATAATTGATTCTCAACCATTAACCGTGGCAGTGCTTGCAGCCTTCTTATTTGGCGAGTCCATCAGTTTCGTTGGAGCCGCTGGACTAGTACTTGGTGTTTTAGGACTTTTACTTCTTGAGGTAATCCTTTTCCCCTCTGTATAGTTGGGTTTTCTACAGACGTGCACGAGTATGAGACTAACAAAGTGTAATTTTCAACAAACTTATTTATGCAAGTTGTGGCGGGTTCGGCCTTTGGGGAGCTTTTGGGCTGATGAGTCCCAATTTCTTGTAGAAATGAATACTTTTTTTTTTTTTTTTTTTTTGAAAACCTTAATTTCGTAATGCTCTGCCCTCAAAATGAAGAGAGCTTCTGAATTAAAAATTTATGCTGAAATTCAAGAACCATTTAACATACACTTCTGCATGTTCTGAACTGTGATCTGATCTGATCTGATCAAATCTTGATATTTCAATTTCACTATTCATACTCTTTGTAAACTTTAATTGCATCATCTTATGAACGTGCAGCTTGGCTTCTGGCCTCTTTTAAAGAAAACACTTTTTTTTTTCCTTTTCCACAATAGAATAATTGTTAGTGTGTGTGTGTGAATGTCTTTATATTCTTTTCTACATTATGTTAGTATGATTGTTCGAGAAATTTATTAAATGAAAACAGATGTGACATTCATGAGACTAATCTATATGCACTTTATCAAAACATAGCTTCTGTTTGATATGGCACTCGGTTTGACAGGTTCCTTCACTTTCTTTAGATGCAAGTAGCTTTTCGTTATGGGGAAGTGGAGAGTGGTGGATGTTTCTAGCTGCACAGAGCATGGCAGTAGGTACTGTCATGGTCCGCTGGGTTTCCAAGTACTCTGATCCTATTATGGCAACTGGATGGGTAGGATTAATGAAGTGATCTTTTATGAATTTACATCATTGTTCTTTCTTATGCCTTTTGTAGATTATTTTAGTGTACCATTTTTGTCATCCTTATTCAAAATCTATATGATCATATGATTGGAAGAAAATTAGTTTTTAATCTACCTTTCGCGCACTGTTCATTGTCATCATGGGAAATCTCCTGCCCTCTTCACCCAATTAACCCATTGTGTTATGATTTTGACAGATCCGAAGAGAGTTCAGTTATGATTTAGATTAATTCTTGGTGTCTTTGCTTTGTACTTTCTCTAATGTCTAATTCTAGGAAGTGTTGTCAGTGTAGCATGCACGCAATTAAATGTTGAAAGCATCAAACTTCTATGGTTCTGTTATTGGAAGTATGAAACACTTGAAGGATGCCCTATAGAGGCCTTAGTCTATTGAAGGAAATAAAGCATGGAACTAAATAAATGTAACTTTTTGTTGCAGCACATGGTGATTGGTGGTCTCCCGCTTTTGATGATCTGTTTCCTTAATCACGATCCTGCCGTCAGTGGGAGCCTTAAGGATTTTACAACAAATGATATACTGGCGCTCCTTTATGCATCCATTTTTGGAAGTGCTGTTAGCTATGGTTCATTCTTCTATAGTGCAACAAAAGGTTTCACACTCTTTTCCTCGTAGCGTTTTCTAGTTTGTATGGGCGTTTTGCTTTGAAGTTCAAATTGTCTCATCTTGTCGCTTTATTATTATTTTTTCTAATGTTACACGTGACTTCATGCTTTGGTTGATGGCTTAATCATTGATTTAATATTTTTCCCACGTGCATACATAATTTGGAGTTTTCAATTTAAATACCATGTAAACTATAATGAAAGAAAAAGAATATAAGGTAACCCCTTACAATATATTGATATTCAAGGGGAAAAGATAGCCCAACGGCAATACAAAACTCCCAGCAAATTCTAGAGTGCTGGTGCTCTCCCCTCTCTTTCAGCTTCAAGCTAAAAACACACATAAACCCTAAAATGAAAACATAACAACTATATATACTACCCACCATAGGTCCAACTCTCATCAGCCCTTACTCTCTCTTGGGACCACAGCAGCCCCCCACAAACTTCTGCACCACTACCCACATAACTAACTCACTGTGGAATTCCCCTTTTTACCCTTCCTAGTATAAACCTTAGTAATAGGAGGCCTAACAAAGAATTACCAGACTCCTATAAGTCTATAACTAGGATACTTACACACACTCACGCACACAACAATATATACATGCTGAAATTCGATCTATTTGAAATTTGAGTATAAGTTGTTGATGCATTTATGATAATTTAATTATTGGAGTTTTCAATTTTATAAGAATGCTCAAAGACACAATGATAACATCACTTTATAAGTCTCACTGTGCTGGATTAATAAACATTGTGAAATCCATTTAGTTTCATCATGGTTTACTCATTTTTTGATATGCTGTGATCTCTATTTTGGTGCTAATTCTCCATTGTGGATGTTTTTCTAATAGGTAGTTTGACAAAGCTCAGCTCTCTCACTTTTCTCACCCCAATGTTTGCTTCAGTTTTTGGGTAAGGTTCTTCCCATCATTTTTCAACTGGGAACAAAGACAATGAGTGAAATATGTACTTTATAGACACTTCAATGTTTCCTCTGTTCGTTTATCAACTCAGGTTTCTATATTTGGGCGAGACATTCTCACCTATTCAACTGGTTGGAGCCGTTGTCACTGTGGTTGCTATATATGTGGTCAACTATGGCAGTAGTTTGGAATGA

mRNA sequence

ATGGCGGCCTCTTGGCCCTCCATCTCTTCCTGTTTAGCCACTGCCACACTCATTTCCTCTTCTTCTCCGTCCGACTCTCGCCCTCTTCTTCATTTCCGTTTCACCAGACGAATTCTCGCACCGGACATTTCCTCCGCCGCCCCAATCCTCCGGCGACGAGTGCCGTCTCATTTGGGTTTCAGAAGATACGGTGAGAATGACTGGTTTCGCTCCGATTATGTTGCAATTCCGGTGGTGAATTGTACCAGAAGTGGTGCGGATACCGAGTTGGATTTGTCTGAGTCCATCGAATGCGTGGGGACTGCTCAGGATGTGGAGTGCGTGGTTTCATCGAGTGATGAAGTTGAAGATCCTCTGTCCTCAATTGGGGAGTCACTGGAATTAGGGTTTTCTTCCGATGTTGTTGGTGGTGGTGGTGATGGCTCTGTGGCAGTCTTGGAGAAAGCCTGGGAGTTCGCCGTGTTGGTTTCGCCGTTTTTCTTTTGGGGTACGGCTATGGTGGCGATGAAAGAGGTGCTTCCAAGGTCTGGTCCCTTTTTCGTTTCCGCCTTTCGTCTTATACCTGCCGGTTTCCTTTTGATTGCCTTTGCTGCTTTCCGCGGTCGCCCCTTTCCCTCTGGTTTTTCTGCTTGGATTTCCATCCTTCTCTTTGCTCTTGTCGACGCTACATCGTTTCAGGGCTTTCTTGCTCAAGGCTTGCAGAGGACATCCGCAGGCTTGGGCAGTGTAATAATTGATTCTCAACCATTAACCGTGGCAGTGCTTGCAGCCTTCTTATTTGGCGAGTCCATCAGTTTCGTTGGAGCCGCTGGACTAGTACTTGGTGTTTTAGGACTTTTACTTCTTGAGGTTCCTTCACTTTCTTTAGATGCAAGTAGCTTTTCGTTATGGGGAAGTGGAGAGTGGTGGATGTTTCTAGCTGCACAGAGCATGGCAGTAGGTACTGTCATGGTCCGCTGGGTTTCCAAGTACTCTGATCCTATTATGGCAACTGGATGGCACATGGTGATTGGTGGTCTCCCGCTTTTGATGATCTGTTTCCTTAATCACGATCCTGCCGTCAGTGGGAGCCTTAAGGATTTTACAACAAATGATATACTGGCGCTCCTTTATGCATCCATTTTTGGAAGTGCTGTTAGCTATGGTTCATTCTTCTATAGTGCAACAAAAGGTAGTTTGACAAAGCTCAGCTCTCTCACTTTTCTCACCCCAATGTTTGCTTCAGTTTTTGGGTTTCTATATTTGGGCGAGACATTCTCACCTATTCAACTGGTTGGAGCCGTTGTCACTGTGGTTGCTATATATGTGGTCAACTATGGCAGTAGTTTGGAATGA

Coding sequence (CDS)

ATGGCGGCCTCTTGGCCCTCCATCTCTTCCTGTTTAGCCACTGCCACACTCATTTCCTCTTCTTCTCCGTCCGACTCTCGCCCTCTTCTTCATTTCCGTTTCACCAGACGAATTCTCGCACCGGACATTTCCTCCGCCGCCCCAATCCTCCGGCGACGAGTGCCGTCTCATTTGGGTTTCAGAAGATACGGTGAGAATGACTGGTTTCGCTCCGATTATGTTGCAATTCCGGTGGTGAATTGTACCAGAAGTGGTGCGGATACCGAGTTGGATTTGTCTGAGTCCATCGAATGCGTGGGGACTGCTCAGGATGTGGAGTGCGTGGTTTCATCGAGTGATGAAGTTGAAGATCCTCTGTCCTCAATTGGGGAGTCACTGGAATTAGGGTTTTCTTCCGATGTTGTTGGTGGTGGTGGTGATGGCTCTGTGGCAGTCTTGGAGAAAGCCTGGGAGTTCGCCGTGTTGGTTTCGCCGTTTTTCTTTTGGGGTACGGCTATGGTGGCGATGAAAGAGGTGCTTCCAAGGTCTGGTCCCTTTTTCGTTTCCGCCTTTCGTCTTATACCTGCCGGTTTCCTTTTGATTGCCTTTGCTGCTTTCCGCGGTCGCCCCTTTCCCTCTGGTTTTTCTGCTTGGATTTCCATCCTTCTCTTTGCTCTTGTCGACGCTACATCGTTTCAGGGCTTTCTTGCTCAAGGCTTGCAGAGGACATCCGCAGGCTTGGGCAGTGTAATAATTGATTCTCAACCATTAACCGTGGCAGTGCTTGCAGCCTTCTTATTTGGCGAGTCCATCAGTTTCGTTGGAGCCGCTGGACTAGTACTTGGTGTTTTAGGACTTTTACTTCTTGAGGTTCCTTCACTTTCTTTAGATGCAAGTAGCTTTTCGTTATGGGGAAGTGGAGAGTGGTGGATGTTTCTAGCTGCACAGAGCATGGCAGTAGGTACTGTCATGGTCCGCTGGGTTTCCAAGTACTCTGATCCTATTATGGCAACTGGATGGCACATGGTGATTGGTGGTCTCCCGCTTTTGATGATCTGTTTCCTTAATCACGATCCTGCCGTCAGTGGGAGCCTTAAGGATTTTACAACAAATGATATACTGGCGCTCCTTTATGCATCCATTTTTGGAAGTGCTGTTAGCTATGGTTCATTCTTCTATAGTGCAACAAAAGGTAGTTTGACAAAGCTCAGCTCTCTCACTTTTCTCACCCCAATGTTTGCTTCAGTTTTTGGGTTTCTATATTTGGGCGAGACATTCTCACCTATTCAACTGGTTGGAGCCGTTGTCACTGTGGTTGCTATATATGTGGTCAACTATGGCAGTAGTTTGGAATGA

Protein sequence

MAASWPSISSCLATATLISSSSPSDSRPLLHFRFTRRILAPDISSAAPILRRRVPSHLGFRRYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLSSIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYVVNYGSSLE
Homology
BLAST of Lcy09g012980 vs. ExPASy Swiss-Prot
Match: Q93V85 (WAT1-related protein At3g02690, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g02690 PE=1 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 3.7e-132
Identity = 250/347 (72.05%), Postives = 284/347 (81.84%), Query Frame = 0

Query: 93  SESIECVGTAQDVECVVSSSDEVEDPLSSIGESLELGFSSDVVGGGGDGSVAVLEKAWEF 152
           S S++CVG   DVECV +  DE         E+   G  S     GG+G+        E+
Sbjct: 86  SSSVDCVGMGSDVECVNNGEDE---------ENRSSGILS-----GGEGTFL------EW 145

Query: 153 AVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWI 212
            VL+SPFFFWGTAMVAMKEVLP +GPFFV+AFRLIPAG LL+AFA ++GRP P G +AW 
Sbjct: 146 TVLISPFFFWGTAMVAMKEVLPITGPFFVAAFRLIPAGLLLVAFAVYKGRPLPEGINAWF 205

Query: 213 SILLFALVDATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGL 272
           SI LFALVDAT FQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLA+FLFGESI  V A GL
Sbjct: 206 SIALFALVDATCFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLASFLFGESIGIVRAGGL 265

Query: 273 VLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATG 332
           +LGV GLLLLEVPS++ D ++FSLWGSGEWWM LAAQSMA+GTVMVRWVSKYSDPIMATG
Sbjct: 266 LLGVAGLLLLEVPSVTSDGNNFSLWGSGEWWMLLAAQSMAIGTVMVRWVSKYSDPIMATG 325

Query: 333 WHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDILALLYASIFGSAVSYGSFFYSATKGS 392
           WHMVIGGLPLL I  +NHDP  +GSL+D +TND++ALLY SIFGSAVSYG +FYSATKGS
Sbjct: 326 WHMVIGGLPLLAISVINHDPVFNGSLQDLSTNDVIALLYTSIFGSAVSYGVYFYSATKGS 385

Query: 393 LTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYVVNY 440
           LTKLSSLTFLTPMFAS+FG+LYL ETFS +QLVGA VT+VAIY+VN+
Sbjct: 386 LTKLSSLTFLTPMFASIFGYLYLNETFSSLQLVGAAVTLVAIYLVNF 412

BLAST of Lcy09g012980 vs. ExPASy Swiss-Prot
Match: P74436 (Uncharacterized transporter sll0355 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll0355 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.1e-71
Identity = 157/302 (51.99%), Postives = 208/302 (68.87%), Query Frame = 0

Query: 155 LVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISI 214
           L++PFF WGTAMVAMK VL  + PFFV+  RLIPAG L++ +A  + RP P  +  W  I
Sbjct: 17  LIAPFFLWGTAMVAMKGVLADTTPFFVATVRLIPAGILVLLWAMGQKRPQPQNWQGWGWI 76

Query: 215 LLFALVDATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVL 274
           +LFALVD T FQGFLAQGL+RT AGLGSVIIDSQP+ VA+L+++LF E I  +G  GL+L
Sbjct: 77  ILFALVDGTLFQGFLAQGLERTGAGLGSVIIDSQPIAVALLSSWLFKEVIGGIGWLGLLL 136

Query: 275 GVLGLLLLEVP-----------SLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSK 334
           GV G+ L+ +P            LS++ S  +L  SGE WM LA+ SMAVGTV++ +VS+
Sbjct: 137 GVGGISLIGLPDEWFYQLWHLQGLSINWSGSALGSSGELWMLLASLSMAVGTVLIPFVSR 196

Query: 335 YSDPIMATGWHMVIGGLPLLMICFL-NHDPAVSGSLKDFTTNDILALLYASIFGSAVSYG 394
             DP++ATGWHM+IGGLPLL I  + + +P  +  L  +       L YA++FGSA++YG
Sbjct: 197 RVDPVVATGWHMIIGGLPLLAIALVQDSEPWQNIDLWGWGN-----LAYATVFGSAIAYG 256

Query: 395 SFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYVVNYGSS 445
            FFY A+KG+LT LSSLTFLTP+FA  F  L L E  S +Q +G   T+V+IY++N    
Sbjct: 257 IFFYLASKGNLTSLSSLTFLTPIFALSFSNLILEEQLSSLQWLGVAFTLVSIYLINQREQ 313

BLAST of Lcy09g012980 vs. ExPASy Swiss-Prot
Match: O32256 (Uncharacterized transporter YvbV OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbV PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 6.9e-14
Identity = 80/283 (28.27%), Postives = 122/283 (43.11%), Query Frame = 0

Query: 162 WGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVD 221
           WG      K  L  S P   +  R +  G LL+  A  R          W   L+ AL++
Sbjct: 20  WGVNWPLSKAALAYSPPLLFAGIRTLIGGLLLVIVALPRIHKLRLK-ETWPIYLVSALLN 79

Query: 222 ATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLL 281
            T F G    GL    AGL S I+  QP+ + V +    GES+  +   GL+LG  G+ +
Sbjct: 80  ITLFYGLQTIGLNYLPAGLFSAIVFFQPVLMGVFSWLWLGESMFVMKVIGLILGFAGVAV 139

Query: 282 LEVPSLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLP 341
           +         S       G      +A S A+GTV ++      D I      + IG + 
Sbjct: 140 ISAAGFGGHISVI-----GVLLALGSAVSWALGTVYMKKTGSRVDSIWMVALQLTIGSVF 199

Query: 342 LLMICFLNHDPAVSGSLKDFTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTF 401
           LL+  F       S S   +T   I +LL+ S+F  A+ +  FF     G  +K++S TF
Sbjct: 200 LLISGFWTE----SFSAIQWTAPFITSLLFISVFVIALGWLVFFTLVGSGEASKVASYTF 259

Query: 402 LTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYVVNYGSSLE 445
           L P+ + V   ++L E  +   L G ++ V +I +VN  S  +
Sbjct: 260 LIPLISIVASSIFLHEPLTLSLLAGLLLIVTSICLVNTKSKAQ 292

BLAST of Lcy09g012980 vs. ExPASy Swiss-Prot
Match: O34416 (Uncharacterized transporter YoaV OS=Bacillus subtilis (strain 168) OX=224308 GN=yoaV PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.7e-13
Identity = 69/283 (24.38%), Postives = 128/283 (45.23%), Query Frame = 0

Query: 155 LVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISI 214
           ++S    WG   VAMK  +    P   S  RL      L      + +          S 
Sbjct: 8   IISVTLIWGYTWVAMKVGIHDIPPLLFSGLRLFIGAVPLFLILFIQRKKLSIQKEHLKSY 67

Query: 215 LLFALVDATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVL 274
           ++ +L+    + G L  G+Q   +G  SV++ + P+ V V++ F   E ++     GLV 
Sbjct: 68  IIMSLLMGLGYMGILTYGMQFVDSGKTSVLVYTMPIFVTVISHFSLNEKMNVYKTMGLVC 127

Query: 275 GVLGLL-LLEVPSLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGW 334
           G+ GLL +     L++D S+      GE  + +AA S  +  V  +   K+ D I    W
Sbjct: 128 GLFGLLFIFGKEMLNIDQSAL----FGELCVLVAALSWGIANVFSKLQFKHIDIIHMNAW 187

Query: 335 HMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDILALLYASIFGSAVSYGSFFYSATKGSL 394
           H+++G + LL+  F+    AV  +  ++T   + +LL+  +  +  ++  +F+   +   
Sbjct: 188 HLMMGAVMLLVFSFIFE--AVPSA--EWTYQAVWSLLFNGLLSTGFTFVVWFWVLNQIQA 247

Query: 395 TKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYV 437
           +K S      P+ A  FG+L L E  +   ++GA++    I++
Sbjct: 248 SKASMALMFVPVLALFFGWLQLHEQITINIILGALLICCGIFM 282

BLAST of Lcy09g012980 vs. ExPASy Swiss-Prot
Match: P42194 (Protein PecM OS=Dickeya dadantii (strain 3937) OX=198628 GN=pecM PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.7e-12
Identity = 82/283 (28.98%), Postives = 124/283 (43.82%), Query Frame = 0

Query: 162 WGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVD 221
           WGT      + LP   P   +  R +PAG +LI      G+  P     W   +L AL +
Sbjct: 14  WGTTYFVTTQFLPADKPLLAALIRALPAGIILIL-----GKNLPPVGWLWRLFVLGAL-N 73

Query: 222 ATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGL-L 281
              F   L     R   G+ +++   QPL V +L+  L  + +        V G +G+ L
Sbjct: 74  IGVFFVMLFFAAYRLPGGVVALVGSLQPLIVILLSFLLLTQPVLKKQMVAAVAGGIGIVL 133

Query: 282 LLEVPSLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSKYSDP-----IMATGWHM 341
           L+ +P   L+        +G     LA  SMA G V+ +   K+  P     +  TGW +
Sbjct: 134 LISLPKAPLNP-------AGLVASALATMSMASGLVLTK---KWGRPAGMTMLTFTGWQL 193

Query: 342 VIGGLPLLMICFLNHDPAVSGSLKDFTT-NDILALLYASIFGSAVSYGSFFYSATKGSLT 401
             GGL +L +  L         L D  T  ++   LY +I GS ++Y  +F      S  
Sbjct: 194 FCGGLVILPVQMLTE------PLPDLVTLTNLAGYLYLAIPGSLLAYFMWFSGLEANSPV 253

Query: 402 KLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYVV 438
            +S L FL+P+ A + GFL+L +  S  QLVG V    A+ +V
Sbjct: 254 IMSLLGFLSPLVALLLGFLFLQQGLSGAQLVGVVFIFSALIIV 274

BLAST of Lcy09g012980 vs. ExPASy TrEMBL
Match: A0A0A0KQD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G429960 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 1.1e-198
Identity = 380/438 (86.76%), Postives = 394/438 (89.95%), Query Frame = 0

Query: 8   ISSCLATATLISSSSPSDSRPLLHFRFTRRILAPDISSAAPILRRRVPSHLGFR-RYGEN 67
           ++ CLATATL + +SPS+S P  H        AP ISSAAPILRRR+P  LGFR RY EN
Sbjct: 1   MAGCLATATL-TPTSPSNSPPFFH--------APPISSAAPILRRRLPFQLGFRTRYDEN 60

Query: 68  DWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLSSIGESL 127
             FR  YVAIPV NCTRSG DTELD +ESI+CVGTAQDVECVVS +D  EDP SSIG  L
Sbjct: 61  SRFRFHYVAIPVANCTRSGGDTELDFTESIDCVGTAQDVECVVSPND--EDPSSSIGVPL 120

Query: 128 ELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL 187
           +LG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL
Sbjct: 121 KLGISSDY---SGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL 180

Query: 188 IPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGLGSVIID 247
           IPAGFLLIAFAAFRGRPFPSGFSAWISI+LFALVDAT FQGFLAQGLQRTSAGLGSVIID
Sbjct: 181 IPAGFLLIAFAAFRGRPFPSGFSAWISIILFALVDATFFQGFLAQGLQRTSAGLGSVIID 240

Query: 248 SQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFL 307
           SQPLTVAVLAAFLFGES+  VGAAGLVLGVLGLLLLEVPSL+ DA+SFSLWGSGEWWMFL
Sbjct: 241 SQPLTVAVLAAFLFGESLGLVGAAGLVLGVLGLLLLEVPSLTFDANSFSLWGSGEWWMFL 300

Query: 308 AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDI 367
           AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMIC LNHDPAVSGSLKDFTTNDI
Sbjct: 301 AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICILNHDPAVSGSLKDFTTNDI 360

Query: 368 LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG 427
           LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG
Sbjct: 361 LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG 420

Query: 428 AVVTVVAIYVVNYGSSLE 445
           AVVTVVAIYVVNYGS LE
Sbjct: 421 AVVTVVAIYVVNYGSGLE 424

BLAST of Lcy09g012980 vs. ExPASy TrEMBL
Match: A0A6J1JVE3 (WAT1-related protein At3g02690, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489226 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.2e-196
Identity = 378/444 (85.14%), Postives = 395/444 (88.96%), Query Frame = 0

Query: 8   ISSCLATATL------ISSSSPSDSRPLLHFRFTRRILAPDISSAAPIL-RRRVPSHLGF 67
           ++ CLATATL      +SS SPS+ RPL  F F R+I+   ISS APIL RRRVP  LGF
Sbjct: 1   MAGCLATATLTTTSPQLSSYSPSNCRPL--FSFRRQIVGLGISSVAPILRRRRVPFQLGF 60

Query: 68  RRYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLS 127
           RRY E   FR DYVAIPV NCTRSGADT+LD +ESI+CVGTAQDVECVVSS+DE      
Sbjct: 61  RRYDEKGRFRVDYVAIPVSNCTRSGADTDLDFTESIDCVGTAQDVECVVSSTDE------ 120

Query: 128 SIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 187
                 ELG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF
Sbjct: 121 ------ELGISSD-FDNDGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 180

Query: 188 VSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGL 247
           VSAFRL+PAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDAT FQGFLAQGLQRTSAGL
Sbjct: 181 VSAFRLVPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATLFQGFLAQGLQRTSAGL 240

Query: 248 GSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSG 307
           GSVIIDSQPLTVAVLAAFLFGESI  VGAAGLVLGVLGL+LLEVPSL+LDASSFSLWGSG
Sbjct: 241 GSVIIDSQPLTVAVLAAFLFGESIGLVGAAGLVLGVLGLILLEVPSLTLDASSFSLWGSG 300

Query: 308 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKD 367
           EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNH+PAVSGSL+D
Sbjct: 301 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHEPAVSGSLQD 360

Query: 368 FTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFS 427
           FTTNDILAL YASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFAS+FGFLYLGETFS
Sbjct: 361 FTTNDILALFYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASIFGFLYLGETFS 420

Query: 428 PIQLVGAVVTVVAIYVVNYGSSLE 445
           PIQLVGAVVTVV+IYVVNYGSSLE
Sbjct: 421 PIQLVGAVVTVVSIYVVNYGSSLE 429

BLAST of Lcy09g012980 vs. ExPASy TrEMBL
Match: A0A6J1FGD0 (WAT1-related protein At3g02690, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445247 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.9e-196
Identity = 377/444 (84.91%), Postives = 394/444 (88.74%), Query Frame = 0

Query: 8   ISSCLATATL------ISSSSPSDSRPLLHFRFTRRILAPDISSAAPIL-RRRVPSHLGF 67
           ++ CLAT TL      +SSSSPS+ RPL  F F R+I+   ISS APIL RRRVP  LGF
Sbjct: 1   MAGCLATVTLTTTSLQLSSSSPSNCRPL--FSFRRQIVGLGISSVAPILRRRRVPFQLGF 60

Query: 68  RRYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLS 127
           RRY E   FR DYVAIP +NCTRSGADT+LD +ESI+CVGTAQDVECVVSS+DE      
Sbjct: 61  RRYDEKGRFRVDYVAIPALNCTRSGADTDLDFTESIDCVGTAQDVECVVSSTDE------ 120

Query: 128 SIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 187
                 ELG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF
Sbjct: 121 ------ELGISSD-FDNDGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 180

Query: 188 VSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGL 247
           VSAFRL+PAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDAT FQGFLAQGLQRTSAGL
Sbjct: 181 VSAFRLVPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATLFQGFLAQGLQRTSAGL 240

Query: 248 GSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSG 307
           GSVIIDSQPLTVAVLAAFLFGESI  VGAAGLVLGVLGLLLLEVPSL+LDASSFSLWGSG
Sbjct: 241 GSVIIDSQPLTVAVLAAFLFGESIGLVGAAGLVLGVLGLLLLEVPSLTLDASSFSLWGSG 300

Query: 308 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKD 367
           EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLL ICFLNH+PAVSGSL+D
Sbjct: 301 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLAICFLNHEPAVSGSLQD 360

Query: 368 FTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFS 427
           FTTNDILAL YASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFAS+FGFLYLGETFS
Sbjct: 361 FTTNDILALFYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASIFGFLYLGETFS 420

Query: 428 PIQLVGAVVTVVAIYVVNYGSSLE 445
           PIQLVGAVVTVV+IYVVNYGSSLE
Sbjct: 421 PIQLVGAVVTVVSIYVVNYGSSLE 429

BLAST of Lcy09g012980 vs. ExPASy TrEMBL
Match: A0A1S3CK55 (WAT1-related protein At3g02690, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501378 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.9e-196
Identity = 377/435 (86.67%), Postives = 390/435 (89.66%), Query Frame = 0

Query: 11  CLATATLISSSSPSDSRPLLHFRFTRRILAPDISSAAPILRRRVPSHLGF-RRYGENDWF 70
           CLATATL + +SPS+S P   FR      AP ISSA PILRRR+P  LGF  RY EN  F
Sbjct: 4   CLATATL-TPTSPSNSPPF--FR------APSISSAPPILRRRLPFRLGFGTRYDENPLF 63

Query: 71  RSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLSSIGESLELG 130
           R  YVAIPV NCTRSG DTELD +ESI+CVGTAQDVECVVS +D  EDP SSIG  LELG
Sbjct: 64  RFHYVAIPVANCTRSGGDTELDFTESIDCVGTAQDVECVVSPTD--EDPSSSIGVPLELG 123

Query: 131 FSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPA 190
            SSD  GG   GSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPA
Sbjct: 124 ISSDYSGG---GSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPA 183

Query: 191 GFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGLGSVIIDSQP 250
           GFLL+AFAAFRGRPFPSGFSAWISI+LFALVDAT FQGFLAQGLQRTSAGLGSVIIDSQP
Sbjct: 184 GFLLVAFAAFRGRPFPSGFSAWISIILFALVDATFFQGFLAQGLQRTSAGLGSVIIDSQP 243

Query: 251 LTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFLAAQ 310
           LTVAVLAAFLFGES+  +GAAGLVLGV GLLLLEVPSL+ DA+SFSLWGSGEWWMFLAAQ
Sbjct: 244 LTVAVLAAFLFGESLGLIGAAGLVLGVFGLLLLEVPSLTFDANSFSLWGSGEWWMFLAAQ 303

Query: 311 SMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDILAL 370
           SMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMIC LNHDPAVSGSLKDFTTNDILAL
Sbjct: 304 SMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICILNHDPAVSGSLKDFTTNDILAL 363

Query: 371 LYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVV 430
           LYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVV
Sbjct: 364 LYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVV 423

Query: 431 TVVAIYVVNYGSSLE 445
           TVVAIY VNYGSSLE
Sbjct: 424 TVVAIYAVNYGSSLE 424

BLAST of Lcy09g012980 vs. ExPASy TrEMBL
Match: A0A6J1CI41 (WAT1-related protein At3g02690, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011840 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 8.4e-196
Identity = 376/446 (84.30%), Postives = 394/446 (88.34%), Query Frame = 0

Query: 1   MAASWPSISSCLATATLISSSSPSDSRPLLHFRFTRR-ILAPDISSAAPILRRRVPSHLG 60
           MA  WPS SS L T        PS+SRPLLHF F+R+ +LA   SS API+ RRV  H  
Sbjct: 29  MAGFWPSASSSLPT--------PSNSRPLLHFSFSRQLLLASPNSSPAPIVHRRVGFHFS 88

Query: 61  FR-RYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDP 120
              RYG NDWFR+DYV IPVVNCTRSGADTELDLSES++CVGTAQDVECVVS +D  EDP
Sbjct: 89  STGRYGHNDWFRADYVPIPVVNCTRSGADTELDLSESVDCVGTAQDVECVVSPAD--EDP 148

Query: 121 LSSIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGP 180
            SSI         +DV    GDGS+AVL KAWEFAVLVSPFFFWGTAMVAMKEVLPRSGP
Sbjct: 149 RSSI---------ADV---DGDGSLAVLGKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGP 208

Query: 181 FFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSA 240
           FFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISI LFALVDATSFQGFLAQGLQRTSA
Sbjct: 209 FFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISIALFALVDATSFQGFLAQGLQRTSA 268

Query: 241 GLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWG 300
           GLGSVIIDSQPLTVAVLAA LFGESIS +GAAGL+LGVLGLLLLEVPSL+LDAS+FSLWG
Sbjct: 269 GLGSVIIDSQPLTVAVLAALLFGESISLIGAAGLLLGVLGLLLLEVPSLALDASTFSLWG 328

Query: 301 SGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSL 360
           SGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLL IC LNHDPA+SGSL
Sbjct: 329 SGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLAICILNHDPAISGSL 388

Query: 361 KDFTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGET 420
           KDFTTNDILALLYASIFGSA+SYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGET
Sbjct: 389 KDFTTNDILALLYASIFGSAISYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGET 448

Query: 421 FSPIQLVGAVVTVVAIYVVNYGSSLE 445
           FSPIQLVGAVVTVVAIYVVNYGSS+E
Sbjct: 449 FSPIQLVGAVVTVVAIYVVNYGSSVE 452

BLAST of Lcy09g012980 vs. NCBI nr
Match: XP_038882098.1 (WAT1-related protein At3g02690, chloroplastic [Benincasa hispida])

HSP 1 Score: 721.1 bits (1860), Expect = 5.9e-204
Identity = 386/438 (88.13%), Postives = 403/438 (92.01%), Query Frame = 0

Query: 8   ISSCLATAT-LISSSSPSDSRPLLHFRFTRRILAPDISSAAPILRRRVPSHLGFRRYGEN 67
           ++ CLATAT   +S+SPS+SRPLLHF FTR+IL P ISSAAPILRRR P HLGF RYGEN
Sbjct: 1   MAGCLATATPTPTSTSPSNSRPLLHFSFTRQILTPHISSAAPILRRRHPFHLGFTRYGEN 60

Query: 68  DWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLSSIGESL 127
             FR DYVAIPV NCTRSGADTELDL+ESI+CVGTAQDVECV+SS+   EDP SS+    
Sbjct: 61  ARFRFDYVAIPVANCTRSGADTELDLTESIDCVGTAQDVECVISSTG--EDPPSSV---- 120

Query: 128 ELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL 187
           E G SS+     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL
Sbjct: 121 ESGVSSEF---DGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL 180

Query: 188 IPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGLGSVIID 247
           IPAGFLLIAFAAFRGRPFPSGFSAWISI+LFALVDAT FQGFLAQGLQRTSAGLGSVIID
Sbjct: 181 IPAGFLLIAFAAFRGRPFPSGFSAWISIILFALVDATFFQGFLAQGLQRTSAGLGSVIID 240

Query: 248 SQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFL 307
           SQPLTVAVLAAFLFGESI  VGAAGLVLGVLGLLLLEVPSL+ DA+SFSLWGSGEWWMFL
Sbjct: 241 SQPLTVAVLAAFLFGESIGLVGAAGLVLGVLGLLLLEVPSLTFDANSFSLWGSGEWWMFL 300

Query: 308 AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDI 367
           AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMIC LNHDPAVSGSLKDFTTNDI
Sbjct: 301 AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICILNHDPAVSGSLKDFTTNDI 360

Query: 368 LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG 427
           LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFAS FGFLYLGETFSPIQLVG
Sbjct: 361 LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASAFGFLYLGETFSPIQLVG 420

Query: 428 AVVTVVAIYVVNYGSSLE 445
           AVVTVVAIYVVNYG++LE
Sbjct: 421 AVVTVVAIYVVNYGNTLE 429

BLAST of Lcy09g012980 vs. NCBI nr
Match: XP_004140354.1 (WAT1-related protein At3g02690, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 702.6 bits (1812), Expect = 2.2e-198
Identity = 380/438 (86.76%), Postives = 394/438 (89.95%), Query Frame = 0

Query: 8   ISSCLATATLISSSSPSDSRPLLHFRFTRRILAPDISSAAPILRRRVPSHLGFR-RYGEN 67
           ++ CLATATL + +SPS+S P  H        AP ISSAAPILRRR+P  LGFR RY EN
Sbjct: 1   MAGCLATATL-TPTSPSNSPPFFH--------APPISSAAPILRRRLPFQLGFRTRYDEN 60

Query: 68  DWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLSSIGESL 127
             FR  YVAIPV NCTRSG DTELD +ESI+CVGTAQDVECVVS +D  EDP SSIG  L
Sbjct: 61  SRFRFHYVAIPVANCTRSGGDTELDFTESIDCVGTAQDVECVVSPND--EDPSSSIGVPL 120

Query: 128 ELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL 187
           +LG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL
Sbjct: 121 KLGISSDY---SGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRL 180

Query: 188 IPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGLGSVIID 247
           IPAGFLLIAFAAFRGRPFPSGFSAWISI+LFALVDAT FQGFLAQGLQRTSAGLGSVIID
Sbjct: 181 IPAGFLLIAFAAFRGRPFPSGFSAWISIILFALVDATFFQGFLAQGLQRTSAGLGSVIID 240

Query: 248 SQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFL 307
           SQPLTVAVLAAFLFGES+  VGAAGLVLGVLGLLLLEVPSL+ DA+SFSLWGSGEWWMFL
Sbjct: 241 SQPLTVAVLAAFLFGESLGLVGAAGLVLGVLGLLLLEVPSLTFDANSFSLWGSGEWWMFL 300

Query: 308 AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDI 367
           AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMIC LNHDPAVSGSLKDFTTNDI
Sbjct: 301 AAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICILNHDPAVSGSLKDFTTNDI 360

Query: 368 LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG 427
           LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG
Sbjct: 361 LALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVG 420

Query: 428 AVVTVVAIYVVNYGSSLE 445
           AVVTVVAIYVVNYGS LE
Sbjct: 421 AVVTVVAIYVVNYGSGLE 424

BLAST of Lcy09g012980 vs. NCBI nr
Match: KAG6579056.1 (WAT1-related protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 700.7 bits (1807), Expect = 8.3e-198
Identity = 379/444 (85.36%), Postives = 396/444 (89.19%), Query Frame = 0

Query: 8   ISSCLATATL------ISSSSPSDSRPLLHFRFTRRILAPDISSAAPIL-RRRVPSHLGF 67
           ++ CLAT TL      +SSSSPS+ RPL  F F R+I+   ISS APIL RRRVP  LGF
Sbjct: 1   MAGCLATVTLTTTSLQLSSSSPSNCRPL--FSFRRQIVGLGISSVAPILRRRRVPFQLGF 60

Query: 68  RRYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLS 127
           RRY E  WFR DYVAIPV+NCTRSGADT+LD +ESI+CVGTAQDVECVVSS+DE      
Sbjct: 61  RRYDEKGWFRVDYVAIPVLNCTRSGADTDLDFTESIDCVGTAQDVECVVSSTDE------ 120

Query: 128 SIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 187
                 ELG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF
Sbjct: 121 ------ELGISSD-FDNDGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 180

Query: 188 VSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGL 247
           VSAFRL+PAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDAT FQGFLAQGLQRTSAGL
Sbjct: 181 VSAFRLVPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATLFQGFLAQGLQRTSAGL 240

Query: 248 GSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSG 307
           GSVIIDSQPLTVAVLAAFLFGESI  VGAAGLVLGVLGLLLLEVPSL+LDASSFSLWGSG
Sbjct: 241 GSVIIDSQPLTVAVLAAFLFGESIGLVGAAGLVLGVLGLLLLEVPSLTLDASSFSLWGSG 300

Query: 308 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKD 367
           EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLL ICFLNH+PAVSGSL+D
Sbjct: 301 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLAICFLNHEPAVSGSLQD 360

Query: 368 FTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFS 427
           FTTNDILAL YASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFAS+FGFLYLGETFS
Sbjct: 361 FTTNDILALFYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASIFGFLYLGETFS 420

Query: 428 PIQLVGAVVTVVAIYVVNYGSSLE 445
           PIQLVGAVVTVV+IYVVNYGSSLE
Sbjct: 421 PIQLVGAVVTVVSIYVVNYGSSLE 429

BLAST of Lcy09g012980 vs. NCBI nr
Match: KAG7016588.1 (WAT1-related protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 695.3 bits (1793), Expect = 3.5e-196
Identity = 378/444 (85.14%), Postives = 395/444 (88.96%), Query Frame = 0

Query: 8   ISSCLATATL------ISSSSPSDSRPLLHFRFTRRILAPDISSAAPIL-RRRVPSHLGF 67
           ++ CLAT TL      +SSSSPS+ RPL  F F R+I+   ISS APIL RRRVP  LGF
Sbjct: 1   MAGCLATVTLTTTSLQLSSSSPSNCRPL--FSFRRQIVGLGISSVAPILRRRRVPFQLGF 60

Query: 68  RRYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLS 127
           RRY E   FR DYVAIPV+NCTRSGADT+LD +ESI+CVGTAQDVECVVSS+DE      
Sbjct: 61  RRYDEKGRFRVDYVAIPVLNCTRSGADTDLDFTESIDCVGTAQDVECVVSSTDE------ 120

Query: 128 SIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 187
                 ELG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF
Sbjct: 121 ------ELGISSD-FDNDGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 180

Query: 188 VSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGL 247
           VSAFRL+PAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDAT FQGFLAQGLQRTSAGL
Sbjct: 181 VSAFRLVPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATLFQGFLAQGLQRTSAGL 240

Query: 248 GSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSG 307
           GSVIIDSQPLTVAVLAAFLFGESI  VGAAGLVLGVLGLLLLEVPSL+LDASSFSLWGSG
Sbjct: 241 GSVIIDSQPLTVAVLAAFLFGESIGLVGAAGLVLGVLGLLLLEVPSLTLDASSFSLWGSG 300

Query: 308 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKD 367
           EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLL ICFLNH+PAVSGSL+D
Sbjct: 301 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLAICFLNHEPAVSGSLQD 360

Query: 368 FTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFS 427
           FTTNDILAL YASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFAS+FGFLYLGETFS
Sbjct: 361 FTTNDILALFYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASIFGFLYLGETFS 420

Query: 428 PIQLVGAVVTVVAIYVVNYGSSLE 445
           PIQLVGAVVTVV+IYVVNYGSSLE
Sbjct: 421 PIQLVGAVVTVVSIYVVNYGSSLE 429

BLAST of Lcy09g012980 vs. NCBI nr
Match: XP_022993106.1 (WAT1-related protein At3g02690, chloroplastic [Cucurbita maxima])

HSP 1 Score: 694.9 bits (1792), Expect = 4.5e-196
Identity = 378/444 (85.14%), Postives = 395/444 (88.96%), Query Frame = 0

Query: 8   ISSCLATATL------ISSSSPSDSRPLLHFRFTRRILAPDISSAAPIL-RRRVPSHLGF 67
           ++ CLATATL      +SS SPS+ RPL  F F R+I+   ISS APIL RRRVP  LGF
Sbjct: 1   MAGCLATATLTTTSPQLSSYSPSNCRPL--FSFRRQIVGLGISSVAPILRRRRVPFQLGF 60

Query: 68  RRYGENDWFRSDYVAIPVVNCTRSGADTELDLSESIECVGTAQDVECVVSSSDEVEDPLS 127
           RRY E   FR DYVAIPV NCTRSGADT+LD +ESI+CVGTAQDVECVVSS+DE      
Sbjct: 61  RRYDEKGRFRVDYVAIPVSNCTRSGADTDLDFTESIDCVGTAQDVECVVSSTDE------ 120

Query: 128 SIGESLELGFSSDVVGGGGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 187
                 ELG SSD     GDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF
Sbjct: 121 ------ELGISSD-FDNDGDGSVAVLEKAWEFAVLVSPFFFWGTAMVAMKEVLPRSGPFF 180

Query: 188 VSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATSFQGFLAQGLQRTSAGL 247
           VSAFRL+PAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDAT FQGFLAQGLQRTSAGL
Sbjct: 181 VSAFRLVPAGFLLIAFAAFRGRPFPSGFSAWISILLFALVDATLFQGFLAQGLQRTSAGL 240

Query: 248 GSVIIDSQPLTVAVLAAFLFGESISFVGAAGLVLGVLGLLLLEVPSLSLDASSFSLWGSG 307
           GSVIIDSQPLTVAVLAAFLFGESI  VGAAGLVLGVLGL+LLEVPSL+LDASSFSLWGSG
Sbjct: 241 GSVIIDSQPLTVAVLAAFLFGESIGLVGAAGLVLGVLGLILLEVPSLTLDASSFSLWGSG 300

Query: 308 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHDPAVSGSLKD 367
           EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNH+PAVSGSL+D
Sbjct: 301 EWWMFLAAQSMAVGTVMVRWVSKYSDPIMATGWHMVIGGLPLLMICFLNHEPAVSGSLQD 360

Query: 368 FTTNDILALLYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASVFGFLYLGETFS 427
           FTTNDILAL YASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFAS+FGFLYLGETFS
Sbjct: 361 FTTNDILALFYASIFGSAVSYGSFFYSATKGSLTKLSSLTFLTPMFASIFGFLYLGETFS 420

Query: 428 PIQLVGAVVTVVAIYVVNYGSSLE 445
           PIQLVGAVVTVV+IYVVNYGSSLE
Sbjct: 421 PIQLVGAVVTVVSIYVVNYGSSLE 429

BLAST of Lcy09g012980 vs. TAIR 10
Match: AT3G02690.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 473.0 bits (1216), Expect = 2.6e-133
Identity = 250/347 (72.05%), Postives = 284/347 (81.84%), Query Frame = 0

Query: 93  SESIECVGTAQDVECVVSSSDEVEDPLSSIGESLELGFSSDVVGGGGDGSVAVLEKAWEF 152
           S S++CVG   DVECV +  DE         E+   G  S     GG+G+        E+
Sbjct: 86  SSSVDCVGMGSDVECVNNGEDE---------ENRSSGILS-----GGEGTFL------EW 145

Query: 153 AVLVSPFFFWGTAMVAMKEVLPRSGPFFVSAFRLIPAGFLLIAFAAFRGRPFPSGFSAWI 212
            VL+SPFFFWGTAMVAMKEVLP +GPFFV+AFRLIPAG LL+AFA ++GRP P G +AW 
Sbjct: 146 TVLISPFFFWGTAMVAMKEVLPITGPFFVAAFRLIPAGLLLVAFAVYKGRPLPEGINAWF 205

Query: 213 SILLFALVDATSFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLAAFLFGESISFVGAAGL 272
           SI LFALVDAT FQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLA+FLFGESI  V A GL
Sbjct: 206 SIALFALVDATCFQGFLAQGLQRTSAGLGSVIIDSQPLTVAVLASFLFGESIGIVRAGGL 265

Query: 273 VLGVLGLLLLEVPSLSLDASSFSLWGSGEWWMFLAAQSMAVGTVMVRWVSKYSDPIMATG 332
           +LGV GLLLLEVPS++ D ++FSLWGSGEWWM LAAQSMA+GTVMVRWVSKYSDPIMATG
Sbjct: 266 LLGVAGLLLLEVPSVTSDGNNFSLWGSGEWWMLLAAQSMAIGTVMVRWVSKYSDPIMATG 325

Query: 333 WHMVIGGLPLLMICFLNHDPAVSGSLKDFTTNDILALLYASIFGSAVSYGSFFYSATKGS 392
           WHMVIGGLPLL I  +NHDP  +GSL+D +TND++ALLY SIFGSAVSYG +FYSATKGS
Sbjct: 326 WHMVIGGLPLLAISVINHDPVFNGSLQDLSTNDVIALLYTSIFGSAVSYGVYFYSATKGS 385

Query: 393 LTKLSSLTFLTPMFASVFGFLYLGETFSPIQLVGAVVTVVAIYVVNY 440
           LTKLSSLTFLTPMFAS+FG+LYL ETFS +QLVGA VT+VAIY+VN+
Sbjct: 386 LTKLSSLTFLTPMFASIFGYLYLNETFSSLQLVGAAVTLVAIYLVNF 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93V853.7e-13272.05WAT1-related protein At3g02690, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
P744362.1e-7151.99Uncharacterized transporter sll0355 OS=Synechocystis sp. (strain PCC 6803 / Kazu... [more]
O322566.9e-1428.27Uncharacterized transporter YvbV OS=Bacillus subtilis (strain 168) OX=224308 GN=... [more]
O344167.7e-1324.38Uncharacterized transporter YoaV OS=Bacillus subtilis (strain 168) OX=224308 GN=... [more]
P421941.7e-1228.98Protein PecM OS=Dickeya dadantii (strain 3937) OX=198628 GN=pecM PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KQD41.1e-19886.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G429960 PE=3 SV=1[more]
A0A6J1JVE32.2e-19685.14WAT1-related protein At3g02690, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FGD04.9e-19684.91WAT1-related protein At3g02690, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A1S3CK554.9e-19686.67WAT1-related protein At3g02690, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1CI418.4e-19684.30WAT1-related protein At3g02690, chloroplastic OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
XP_038882098.15.9e-20488.13WAT1-related protein At3g02690, chloroplastic [Benincasa hispida][more]
XP_004140354.12.2e-19886.76WAT1-related protein At3g02690, chloroplastic isoform X3 [Cucumis sativus][more]
KAG6579056.18.3e-19885.36WAT1-related protein, chloroplastic, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG7016588.13.5e-19685.14WAT1-related protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022993106.14.5e-19685.14WAT1-related protein At3g02690, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G02690.12.6e-13372.05nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 299..438
e-value: 1.6E-27
score: 96.3
coord: 156..282
e-value: 1.0E-20
score: 74.3
NoneNo IPR availablePANTHERPTHR32322INNER MEMBRANE TRANSPORTERcoord: 147..442
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 333..442
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 184..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g012980.1Lcy09g012980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane