Lcy09g011930 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g011930
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionbiotin--protein ligase 2-like
LocationChr09: 12982248 .. 12987451 (+)
RNA-Seq ExpressionLcy09g011930
SyntenyLcy09g011930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAATCCAAAAGAATTTTAGAACTCTCAGCCGGCTCAGAAATCTCGGCGGTCTCAGCCAAGTTCATGATTCAAAATCACACTAGAATCAAGATCAAAGAACGGTCATCCCACCAGCCATCTTATTCTTCATCATCTATGGCTTCCAATGCATCTGTGATGCCAATTTATCAACGCGTTTCGTGACTGCAAAGCCCATGTCTTGTTTTTTCCGCACCAGCTCGCAGAATCTTGCAAGGAGGCTCTTCGTAAGAGAAACAAGATGCTCATGACATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAGATTCTCCCTCTCTGAGGCCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGGTTTGTTTCTTGCTTGTTTCAATAAATGCTGGAGTAATTTTCCTGAAAATCTGGGTTCATTACGTTGATTTGGGACGAATTTTACTTTACGGAAGTTGGGTCGAACTGTTTCATAAAGATTTATGCGTTTTTCTGAAAAGAAGCTGAATAACCATATGGGGTATTGGTATTCTTCTGGAAAACAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGTTCTCTCTATTATCTGGTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGTGGCTGAGAATGAAACTGCCAAATTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGATACTGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGAATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCAGTAAGTGTATCGAAGCATTTTTTTTCCTCACCATTTTTTCATTCGTTGACGAGCTAGCTAACTATTAGTTTGCAACAGCAACTTTAGCGACTTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTGTGTAATGCCAATAAGTAACGACAAACTGGTAACATTGTTTGTTAGTTCTGGAATTCCTCTTTCTATTTAAAGTCACTTTGTGAATTTAAGTGAAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTTATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGTAACTTTTGTCATGTTTTGGCCCATATCAGCAACCCTTATGGAATCTTTGTTGTCATTTGGTAACTCGATTCTGGATCCCGTGATATGCTAAATAACCAAAACTTTACGGGTAGGGGATTAAATGAAACTGCAAGAGAAAAACTTATGGTCATGGAAGGAGGAAACAGTTTGTCAGAAAGAATCTGGGGGGACTTGAGGAGTATATAGGATTTCAAGTAGTGGTCGACTGGCTTCCTATAACCAGAACTGTAAACGTACACTATGGAGATGTTTATACTCCATTACATATTTCGTTATTTCTACCGTGCCAATGACACGTAGCTATCACACTCATGTTGTGACTATTATTTGTACGCTTTTGAGGTTAATACCGCTCATTCTCTTTGTGTTTCTATTTCAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTAATTGTGTTTGTTTCCTTTTCCATCTATTTTTCTTCTACAGTCCTTTTTCCCCTTCCAAATTGAAACTTATAGTCTTATTGCACCAATTTGAAAAGCCTAGTGTCGCTGTGATATATTTTAACTGCTGTGTTCTTCCAGGTATAGGTTTGAATGTAGACAACGAAAAACCAACAACATGCTTGAATGAAGCTCTTACAAATTTATCCAGTACACCTTACAAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAACCTGTACGATATTTTTATAAATCAAGGTATTTATATGTCAATCTAACGTCTTCCTCATGATAAGACATTGTCACATTCATGCATAGATTTAGAGCTTCTCCTTCAATCCAACCTTAACAGTGCTCTGGAGATGCTTGTGAGTTATGAAAATTCTAAGCAGCTATCATCATTTGCGAAAAGAATTTGAAATTGTCCATCTCCTCTTCTTTTTCCTCATCACTGAGCATGGTAGTGTGAACATCATTCTATCACTTCATGCTCTTCGTTGCCTTAAGGTTTTGTGCATGCTAAGCATTTGGCAAAGGATAGGACTTCAGATGAATAATTATACGTAAAGGAAATCTTCCTAAGTTTTCTAGTTACTCATCCATCAAGTCTGTAAAGATGATAGCTATATGTAATATTTCCTCTGAAGGGGGTGAGAGAGAGAGCTAAGATATTGTTCGTTTAGGATGTAGATTTTTAATCTTATTCTCTTGTGAAAAAGCTAGAATTTTTGTATCACACGTATTTCACTTATTGTTACCTTCCTAACATGCTGTCATACCTCCAAATATAGTCCACATCATGTTTATCTCAAGTTTGTTATGCTTCAATTTCAGAATTTCCCAGGTGACCTCATTCTGACTAAATCCGTGAATAATGGCATTTTGTTTTCATCATTTCTGAATAATTAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGGTAACTAATGCTGTGGATTAATTTATCTTCTTTATATATATATATATTCAACACATATTGTGTTCCAAGATTCTCACGATTATACTTAGTTTGCATTGTCTATACATTTGTTGTCATGGAAACCAAAAACATCGTGGAAATGGAAAATGTTGTTTGATTCTTTAAGTGCTTAATGTCTATGGAACTAGTAATACTTTGCTTATGTTGCCGTGGGACTCTGTCTTTGCACAAGTCAAACGTCACTTGTTTCAATTGTTTTGTTGACAATTTATGACATATAATATTGTGCAAGTTATGCTAAGTAGGTCTTACACTTTCTTCCCTATAGATATGACCTAAGAAGCATGGACACTTTAGTTTGACTAGCGTATCCGTGTCCGACACTTGTTGGACACGTATCGGACACTTATTAGCACAATTGATATGTAGCAAACACCAGTTGTAGGTTCACTTCGAATCCTTGTTAAGTATACTAAATAGACGCAATACTTAAATGACAAAAATAAAAAAATTTAGGAGTACAATACATTAAACTTATTTTTTTCAAACATACATTGCATGAACTTATTGATTTTGAATTTCCTTTTTGTATAAAAACAATATATATTTTAAAAAATGTATATTTTCTAAACGTGTCCGTGTCCTAGATTTTAAAAAAATGACGTGTTGCCATGTCTGTGTCGTGTCGTATCCGTGTCTCGTGTCCGTATCCGTGCTTCTTAGGATATGACTTTGTTTGGATCTATCTTGTAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGTACGTGATATCTGAATGATAGCTGATCCTGTGGCAGCTCTAGTATTTACACAAATGCTGCAAAGTGCAAACTTAATCCCACTACTCTTTTTGGTTCTTCACTGTGGATATTTATATATTTTTGGCTCAGAAGAAAAGACCTTGTCTCCTGCAGTTGAATTTGTTTTTATCTTGTAGTTAGATAATCCTATTCCTGAAGGATGTTAAATATTCGACATCAATTTGTTGTTCAACTTAGTACTGGAGAATGCTGAATGCACTACATTCTATGATTTTCATGGTTTTAAACACTAAATTCGTTGCTTATTGGTCAGAAATTCTTGTTTTCTTATATAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGGTCAAGTATTTGAATGGATTCCTATTCTTGATTTAGTGAATTAACTATGCTGTGCTGATATTTTTGCAAAGTTTGACACATACAGTTTGATACATCGACAGCGAGTGTACGACATCTGTGTCTATAGCATATCAATTGCATTTTGAAACTAATCCTGAAAGATTGAGAGAGTTCATTGTACATTAAGAAAAGTGACTTTGATATATGTATACCATTTGCCCTAACCTGAACGTTCAAACAACAGATAGGATAACTTGATAAGTCATCTGCTTCAGGCTTTCACCCAAAATCTGGTCACAACATTCTAAAATTTTTTACTTCCTGTTTATACTGTAGATCTGCTTACGAAAAGGAAAAGGTCTATCATAACTAAACTAAATCATTATTATTTACAAAATTGACTGTGAAATTGATTCCAAGTTCATGCATGACTAAGAAACAGTGGTCAATAAAGTTAACGTCTTCAAAGTGCTTTAGAATCAATCATAATGATTATAAAGGAAGTGACTGCCCTGTTGGCCAGTCAACCAATATTGTAGTTTCTGCTGTACTCAGTTGGATGAATTTTTAATTCTGATTTATTTATTGCTCAAAAGTCCTTTAGAACTAAGCTGGCAGCAATATCTACATGCCCTGTTCTGCATTGCATGCATCAGCTCACAACCCCAAGCATTTGAAAATTTTAACCATCTGAATTCTTCTCCAAATGCAATTGACTGTAGAGATCTGATTTTAGAAGGCTAATATGTGTAATAGATCTGATCTTAAATCTGATGATCATGTCATCTTCCTATTGTTCTGCAGTTTGGACTTTTTCAAAGGACTGATCAAGAGAAAACTGGAGTGAGAATGCTGCTTTTTCACTTTCCAACAGGTGGCTGACTATGAGCTGTATGGAATGGAATCATGTGCAATAAGAAGGAGACATTTGCTCTCTTTTATTTTTGGCAAACAATTCTCTGTTTCTAAACTGGAGGAAAGAAAGAGAAAGATATCTTACTTTTTATGATTAAACAATGAGATCTCTACTGAAGTTGTAAATATTATTTATCTTTAAACTTACTAAGGAGGGTCCATGGCCCAGAACGTGCTATATTTTTGTATCATCCTATTGAACGTGAAGGAATTCCGAGTGAGACAATTTCCAATTGATTAAAACTGTCC

mRNA sequence

GCAAATCCAAAAGAATTTTAGAACTCTCAGCCGGCTCAGAAATCTCGGCGGTCTCAGCCAAGTTCATGATTCAAAATCACACTAGAATCAAGATCAAAGAACGGTCATCCCACCAGCCATCTTATTCTTCATCATCTATGGCTTCCAATGCATCTGTGATGCCAATTTATCAACGCGTTTCGTGACTGCAAAGCCCATGTCTTGTTTTTTCCGCACCAGCTCGCAGAATCTTGCAAGGAGGCTCTTCGTAAGAGAAACAAGATGCTCATGACATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAGATTCTCCCTCTCTGAGGCCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGTTCTCTCTATTATCTGGTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGTGGCTGAGAATGAAACTGCCAAATTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGATACTGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGAATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCACAACTTTAGCGACTTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTTATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTATAGGTTTGAATGTAGACAACGAAAAACCAACAACATGCTTGAATGAAGCTCTTACAAATTTATCCAGTACACCTTACAAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAACCTGTACGATATTTTTATAAATCAAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGTTTGGACTTTTTCAAAGGACTGATCAAGAGAAAACTGGAGTGAGAATGCTGCTTTTTCACTTTCCAACAGGTGGCTGACTATGAGCTGTATGGAATGGAATCATGTGCAATAAGAAGGAGACATTTGCTCTCTTTTATTTTTGGCAAACAATTCTCTGTTTCTAAACTGGAGGAAAGAAAGAGAAAGATATCTTACTTTTTATGATTAAACAATGAGATCTCTACTGAAGTTGTAAATATTATTTATCTTTAAACTTACTAAGGAGGGTCCATGGCCCAGAACGTGCTATATTTTTGTATCATCCTATTGAACGTGAAGGAATTCCGAGTGAGACAATTTCCAATTGATTAAAACTGTCC

Coding sequence (CDS)

ATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAGATTCTCCCTCTCTGAGGCCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGTTCTCTCTATTATCTGGTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGTGGCTGAGAATGAAACTGCCAAATTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGATACTGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGAATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCACAACTTTAGCGACTTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTTATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTATAGGTTTGAATGTAGACAACGAAAAACCAACAACATGCTTGAATGAAGCTCTTACAAATTTATCCAGTACACCTTACAAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAACCTGTACGATATTTTTATAAATCAAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGTTTGGACTTTTTCAAAGGACTGATCAAGAGAAAACTGGAGTGA

Protein sequence

MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRKLE
Homology
[Close]
BLAST of Lcy09g011930 vs. ExPASy Swiss-Prot
Match: Q9SL92 (Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 PE=1 SV=2)

HSP 1 Score: 485.0 bits (1247), Expect = 8.2e-136
Identity = 223/345 (64.64%), Postives = 287/345 (83.19%), Query Frame = 0 

Query: 45  NVREKRRLFSLLSGSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLH 104
           +++   RL    S SAME+ ++C+LVL GK+  E E AK LK  ++LKL D+T++S+ L 
Sbjct: 23  SLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 105 SERDKSM--EESGFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVA 164
           SE    +  +++ F +  +MN++ T+ +GRFLIW PR+ STHDV+SHNFS+ P+G+VCV 
Sbjct: 83  SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 165 DVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLP 224
           D+QFKGRGR+KN+WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLP
Sbjct: 143 DIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLP 202

Query: 225 YIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLS 284
           YID+KIKWPNDLYVNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++
Sbjct: 203 YIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMA 262

Query: 285 STPYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVV 344
                L+RE+IL  FF+KFE  +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV
Sbjct: 263 PESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVV 322

Query: 345 ENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRKL 388
           +NVVTIQGLTSSGYLLA+GDD+QM ELHPDGNS DFFKGL++RK+
Sbjct: 323 QNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367

[Close]
BLAST of Lcy09g011930 vs. ExPASy Swiss-Prot
Match: F4I4W2 (Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 6.1e-131
Identity = 214/329 (65.05%), Postives = 274/329 (83.28%), Query Frame = 0 

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSM--EESGFQI 120
           M+  ++C+LVL GK+  E +TA  LK N+ LKL D++++S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 121 DFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWES 180
             +MN++ST+ +GRFLIW P + STHDV+SHNFS+ P+G+VCV+D+Q KGRGR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 181 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 240
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 241 LKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFF 300
           LK+GGILCTSTYRS+KF VS G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 301 NKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLL 360
            KFEN +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 361 AIGDDHQMCELHPDGNSLDFFKGLIKRKL 388
           AIGDD+ M ELHPDGNS DFFKGL++RKL
Sbjct: 301 AIGDDNVMYELHPDGNSFDFFKGLVRRKL 329

[Close]
BLAST of Lcy09g011930 vs. ExPASy Swiss-Prot
Match: P50747 (Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.2e-39
Identity = 90/282 (31.91%), Postives = 154/282 (54.61%), Query Frame = 0 

Query: 116 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFP--LGAVCVADVQFKGRGRSK 175
           F ++ Y   L T   G+ +++    P+T  ++       P  +G + +A  Q +G+GR  
Sbjct: 451 FNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGG 510

Query: 176 NLWESPPGC----LMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIK 235
           N+W SP GC    L+ S  ++ + G+ +P +Q+++S+A+ EA++ I + +    I+L++K
Sbjct: 511 NVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQD---INLRVK 570

Query: 236 WPNDLYVNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLS----ST 295
           WPND+Y + L K+GG+L  ST   + F +  G G NV N  PT C+N+ +T  +    + 
Sbjct: 571 WPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAE 630

Query: 296 PYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVEN 355
              LR + ++A      E L   F ++G  ++  LY R W+HSGQ+V +   +G +    
Sbjct: 631 LKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPK---- 690

Query: 356 VVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRK 387
            V+I GL  SG+L    +  ++  +HPDGNS D  + LI  K
Sbjct: 691 -VSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPK 724

[Close]
BLAST of Lcy09g011930 vs. ExPASy Swiss-Prot
Match: Q920N2 (Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.6e-38
Identity = 90/282 (31.91%), Postives = 147/282 (52.13%), Query Frame = 0 

Query: 116 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFP--LGAVCVADVQFKGRGRSK 175
           F ++ Y   L T   G+ +++     +T  ++     + P  +G + +A  Q +G+GR  
Sbjct: 447 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGP 506

Query: 176 NLWESPPGC----LMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIK 235
           N W SP GC    L+    ++ + G+ +P +Q+++SLA+ EA++ I    G   I+L++K
Sbjct: 507 NAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSI---PGYEDINLRVK 566

Query: 236 WPNDLYVNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTP--- 295
           WPND+Y + L K+GG+L  ST   + F +  G G NV N  PT C+N+ +   +      
Sbjct: 567 WPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLIEEHNKQHGAG 626

Query: 296 -YKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVEN 355
              LR + ++A      E L D F +QG   +  LY + W+H GQ+V +   +G Q    
Sbjct: 627 LKPLRADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWVHGGQQVRLGSTEGPQ---- 686

Query: 356 VVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRK 387
             +I GL  SG+L    +D  +  +HPDGNS D  + LI  K
Sbjct: 687 -ASIVGLDDSGFLQVHQEDGGVVTVHPDGNSFDMLRNLIVPK 720

[Close]
BLAST of Lcy09g011930 vs. ExPASy Swiss-Prot
Match: O14353 (Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bpl1 PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.7e-32
Identity = 99/324 (30.56%), Postives = 159/324 (49.07%), Query Frame = 0 

Query: 96  DTEISVSLHSERDKSMEES--GFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNF-- 155
           D EI    + + +K  +++   F ++ Y   ++   +G  +I  P I ST  ++  N+  
Sbjct: 316 DNEIVKIWYGDEEKICKKAKPSFDLELYAKLINGCRFGLPIIVAPVIRSTQTLLDKNYRF 375

Query: 156 -SDFPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRI----VPLLQYVISL 215
                 G   + + Q  GRGR +N+W SP G L FSF I ++        + L QY+++L
Sbjct: 376 LDSTNTGFTVLGNYQTAGRGRGQNMWVSPYGTLAFSFIINVDAKNFSTTPIALFQYLMAL 435

Query: 216 AITEAIKDICDKKGLPYIDLKIKWPNDLYV------------NGLKVGGILCTSTYRSKK 275
           A+   I++     G   I   IKWPND+YV              +K+ GI+ TS YR   
Sbjct: 436 AVVRGIREYA--PGYENIPAFIKWPNDVYVRVDKGGINFQGKQYMKLSGIIVTSNYRKNV 495

Query: 276 FNVSAGIGLNVDNEKPTTCLNEALT--NLSSTPYKLRR---EDILAFFFNKFENLYDIFI 335
            ++  G G+NV N  PT  LN  +   N +S   +L +   E +LA   N+F+  + + +
Sbjct: 496 LHLVVGCGINVSNLGPTVSLNTLVDEWNKNSDNPRLEKFSFEKLLASVLNQFDRYHRLLL 555

Query: 336 NQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSS-GYLLA--IGDDHQ- 387
            +GF  +   Y + WLHS Q V +    G + +     IQG+TS  G+LLA  + ++++ 
Sbjct: 556 EEGFSLILPEYYQYWLHSNQTVNL--ASGGKAI-----IQGITSDFGFLLAQLLNENNEP 615

[Close]
BLAST of Lcy09g011930 vs. ExPASy TrEMBL
Match: A0A6J1GYI6 (biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 1.9e-191
Identity = 339/387 (87.60%), Postives = 354/387 (91.47%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M LTNPLR FS R SR   SII+SPS R    TTASAPTP TS  V EK RLFSLLSG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL DD EISVSLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS NFSD PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDK+GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. ExPASy TrEMBL
Match: A0A6J1JK17 (biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 4.1e-191
Identity = 339/387 (87.60%), Postives = 351/387 (90.70%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M LTNPLR FS R SR   SII SPS R    TTASAPTP TS  V EK RLFSL SG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           ME SSTCALVLSGKT AENETAKLLKRNDTLKL DD EISVSLHSERDKS++ESGFQI  
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS NFSD PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. ExPASy TrEMBL
Match: A0A1S3BH31 (biotin--protein ligase 2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 4.0e-186
Identity = 320/387 (82.69%), Postives = 353/387 (91.21%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFSL S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL DDTEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISHNFS+ PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. ExPASy TrEMBL
Match: A0A1S3BIK3 (biotin--protein ligase 2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.4e-184
Identity = 320/395 (81.01%), Postives = 353/395 (89.37%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFSL S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL DDTEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISHNFS+ PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVT--------IQGLT 360
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVT        IQGLT
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQTFACFFIQGLT 360

Query: 361 SSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRKL 388
           SSGYLLAIGDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 SSGYLLAIGDDNQMCELHPDGNSLDFFKGLIKSKL 395

[Close]
BLAST of Lcy09g011930 vs. ExPASy TrEMBL
Match: A0A1S4DWM1 (biotin--protein ligase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.3e-180
Identity = 310/383 (80.94%), Postives = 344/383 (89.82%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFSL S S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL DDTEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISHNFS+ PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLI 384
           GDD+QMCELHPDGNS      L+
Sbjct: 361 GDDNQMCELHPDGNSQSMLHALL 383

[Close]
BLAST of Lcy09g011930 vs. NCBI nr
Match: XP_023517017.1 (biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.1 bits (1751), Expect = 2.3e-191
Identity = 338/387 (87.34%), Postives = 354/387 (91.47%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M LTNPLR FS R SR   SII+SPS R    TTASAPTP TS  V EK RLFSLLSG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL DD EIS+SLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISISLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS NFSD PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYV+SLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVVSLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. NCBI nr
Match: XP_022957207.1 (biotin--protein ligase 2-like [Cucurbita moschata])

HSP 1 Score: 678.3 bits (1749), Expect = 3.8e-191
Identity = 339/387 (87.60%), Postives = 354/387 (91.47%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M LTNPLR FS R SR   SII+SPS R    TTASAPTP TS  V EK RLFSLLSG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL DD EISVSLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS NFSD PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDK+GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. NCBI nr
Match: XP_022990857.1 (biotin--protein ligase 2-like [Cucurbita maxima])

HSP 1 Score: 677.2 bits (1746), Expect = 8.6e-191
Identity = 339/387 (87.60%), Postives = 351/387 (90.70%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M LTNPLR FS R SR   SII SPS R    TTASAPTP TS  V EK RLFSL SG A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           ME SSTCALVLSGKT AENETAKLLKRNDTLKL DD EISVSLHSERDKS++ESGFQI  
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS NFSD PLGAVCVADVQFKGRGRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. NCBI nr
Match: KAG6602073.1 (Serine/threonine-protein kinase MPS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.8 bits (1706), Expect = 3.7e-186
Identity = 329/375 (87.73%), Postives = 345/375 (92.00%), Query Frame = 0 

Query: 13   RDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSAMETSSTCALVLS 72
            R SR   SII+SPS R    TTASAPTP TS  V EK RLFSLLSG AMETSSTC+LVLS
Sbjct: 846  RGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLAMETSSTCSLVLS 905

Query: 73   GKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDFYMNALSTNTYGR 132
            GKT AENETAKLLKRNDTLKL DD EISVSLHSERDKS++ESGFQI  YMN+LST+T+GR
Sbjct: 906  GKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFGR 965

Query: 133  FLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQME 192
            FLIWC RIPST DVIS NFSD PLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQME
Sbjct: 966  FLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQME 1025

Query: 193  DGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRS 252
            DGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRS
Sbjct: 1026 DGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRS 1085

Query: 253  KKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNKFENLYDIFINQG 312
            KKFNV+AGIGLN+DN+KPTTCLNEAL NLSSTPYK RREDIL+FFFNKFE LYDIFINQG
Sbjct: 1086 KKFNVTAGIGLNLDNDKPTTCLNEALANLSSTPYKFRREDILSFFFNKFERLYDIFINQG 1145

Query: 313  FQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPD 372
            FQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAIGDD+QMCELHPD
Sbjct: 1146 FQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAIGDDNQMCELHPD 1205

Query: 373  GNSLDFFKGLIKRKL 388
            GNSLDFFKGLIK KL
Sbjct: 1206 GNSLDFFKGLIKSKL 1220

[Close]
BLAST of Lcy09g011930 vs. NCBI nr
Match: XP_038896816.1 (biotin--protein ligase 2-like isoform X1 [Benincasa hispida] >XP_038896824.1 biotin--protein ligase 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 661.4 bits (1705), Expect = 4.9e-186
Identity = 327/387 (84.50%), Postives = 354/387 (91.47%), Query Frame = 0 

Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSLLSGSA 60
           M+L N LRYF RRDSRY+ SI++SPS   LSKT AS PT ETSIN RE+RRLFSLLS S 
Sbjct: 1   MLLANQLRYFGRRDSRYVTSILNSPSFWSLSKTVASVPTLETSINKRERRRLFSLLSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSMEESGFQIDF 120
           M+ + TCALVLSGKTVAENETAKLLKR+DTLKL DDT+ISV LHSERDK +EE+GFQID 
Sbjct: 61  MDANPTCALVLSGKTVAENETAKLLKRSDTLKLPDDTKISVLLHSERDKPLEENGFQIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PRIPST DVISHNFS+ PLGAVCVADVQFKGRGRSKN+WESP 
Sbjct: 121 YLNALSTDTFGRFLIWSPRIPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNVWESPL 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDG IVPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN  K
Sbjct: 181 GCLMFSFTIQMEDGCIVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDQK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KP+T LN ALTNLSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPSTYLNGALTNLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYD+FINQGFQAL+ELY +TWLHSGQRVIV+EKK DQVVENVVTIQGLTSSGYLLA+
Sbjct: 301 FERLYDVFINQGFQALEELYYQTWLHSGQRVIVREKKEDQVVENVVTIQGLTSSGYLLAV 360

Query: 361 GDDHQMCELHPDGNSLDFFKGLIKRKL 388
           GDD+QMCELHPDGNSLDFFKGLIK KL
Sbjct: 361 GDDNQMCELHPDGNSLDFFKGLIKSKL 387

[Close]
BLAST of Lcy09g011930 vs. TAIR 10
Match: AT2G25710.1 (holocarboxylase synthase 1 )

HSP 1 Score: 485.0 bits (1247), Expect = 5.8e-137
Identity = 223/345 (64.64%), Postives = 287/345 (83.19%), Query Frame = 0 

Query: 45  NVREKRRLFSLLSGSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLH 104
           +++   RL    S SAME+ ++C+LVL GK+  E E AK LK  ++LKL D+T++S+ L 
Sbjct: 23  SLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 105 SERDKSM--EESGFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVA 164
           SE    +  +++ F +  +MN++ T+ +GRFLIW PR+ STHDV+SHNFS+ P+G+VCV 
Sbjct: 83  SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 165 DVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLP 224
           D+QFKGRGR+KN+WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLP
Sbjct: 143 DIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLP 202

Query: 225 YIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLS 284
           YID+KIKWPNDLYVNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++
Sbjct: 203 YIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMA 262

Query: 285 STPYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVV 344
                L+RE+IL  FF+KFE  +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV
Sbjct: 263 PESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVV 322

Query: 345 ENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRKL 388
           +NVVTIQGLTSSGYLLA+GDD+QM ELHPDGNS DFFKGL++RK+
Sbjct: 323 QNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367

[Close]
BLAST of Lcy09g011930 vs. TAIR 10
Match: AT2G25710.2 (holocarboxylase synthase 1 )

HSP 1 Score: 485.0 bits (1247), Expect = 5.8e-137
Identity = 223/345 (64.64%), Postives = 287/345 (83.19%), Query Frame = 0 

Query: 45  NVREKRRLFSLLSGSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLH 104
           +++   RL    S SAME+ ++C+LVL GK+  E E AK LK  ++LKL D+T++S+ L 
Sbjct: 23  SLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILE 82

Query: 105 SERDKSM--EESGFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVA 164
           SE    +  +++ F +  +MN++ T+ +GRFLIW PR+ STHDV+SHNFS+ P+G+VCV 
Sbjct: 83  SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 165 DVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLP 224
           D+QFKGRGR+KN+WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLP
Sbjct: 143 DIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLP 202

Query: 225 YIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLS 284
           YID+KIKWPNDLYVNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++
Sbjct: 203 YIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMA 262

Query: 285 STPYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVV 344
                L+RE+IL  FF+KFE  +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV
Sbjct: 263 PESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVV 322

Query: 345 ENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLDFFKGLIKRKL 388
           +NVVTIQGLTSSGYLLA+GDD+QM ELHPDGNS DFFKGL++RK+
Sbjct: 323 QNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367

[Close]
BLAST of Lcy09g011930 vs. TAIR 10
Match: AT1G37150.2 (holocarboxylase synthetase 2 )

HSP 1 Score: 468.8 bits (1205), Expect = 4.3e-132
Identity = 214/329 (65.05%), Postives = 274/329 (83.28%), Query Frame = 0 

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSM--EESGFQI 120
           M+  ++C+LVL GK+  E +TA  LK N+ LKL D++++S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 121 DFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWES 180
             +MN++ST+ +GRFLIW P + STHDV+SHNFS+ P+G+VCV+D+Q KGRGR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 181 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 240
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 241 LKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFF 300
           LK+GGILCTSTYRS+KF VS G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 301 NKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLL 360
            KFEN +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 361 AIGDDHQMCELHPDGNSLDFFKGLIKRKL 388
           AIGDD+ M ELHPDGNS DFFKGL++RKL
Sbjct: 301 AIGDDNVMYELHPDGNSFDFFKGLVRRKL 329

[Close]
BLAST of Lcy09g011930 vs. TAIR 10
Match: AT1G37150.4 (holocarboxylase synthetase 2 )

HSP 1 Score: 406.8 bits (1044), Expect = 2.0e-113
Identity = 181/250 (72.40%), Postives = 220/250 (88.00%), Query Frame = 0 

Query: 138 PRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIV 197
           P + STHDV+SHNFS+ P+G+VCV+D+Q KGRGR+KN+WESP GCLM+SFT++MEDGR+V
Sbjct: 37  PYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMYSFTLEMEDGRVV 96

Query: 198 PLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNV 257
           PL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NGLK+GGILCTSTYRS+KF V
Sbjct: 97  PLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGILCTSTYRSRKFLV 156

Query: 258 SAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNKFENLYDIFINQGFQALQ 317
           S G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF KFEN +D+F+ QGF++L+
Sbjct: 157 SVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFFDLFMEQGFKSLE 216

Query: 318 ELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNSLD 377
           ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLLAIGDD+ M ELHPDGNS D
Sbjct: 217 ELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLLAIGDDNVMYELHPDGNSFD 276

Query: 378 FFKGLIKRKL 388
           FFKGL++RKL
Sbjct: 277 FFKGLVRRKL 286

[Close]
BLAST of Lcy09g011930 vs. TAIR 10
Match: AT1G37150.3 (holocarboxylase synthetase 2 )

HSP 1 Score: 401.0 bits (1029), Expect = 1.1e-111
Identity = 182/291 (62.54%), Postives = 239/291 (82.13%), Query Frame = 0 

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDDTEISVSLHSERDKSM--EESGFQI 120
           M+  ++C+LVL GK+  E +TA  LK N+ LKL D++++S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 121 DFYMNALSTNTYGRFLIWCPRIPSTHDVISHNFSDFPLGAVCVADVQFKGRGRSKNLWES 180
             +MN++ST+ +GRFLIW P + STHDV+SHNFS+ P+G+VCV+D+Q KGRGR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 181 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 240
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 241 LKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFF 300
           LK+GGILCTSTYRS+KF VS G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 301 NKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQ 350
            KFEN +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQ
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQ 291

The following BLAST results are available for this feature:
BLAST of Lcy09g011930 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SL928.2e-13664.64Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 ... [more]
F4I4W26.1e-13165.05Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1[more]
P507474.2e-3931.91Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1[more]
Q920N24.6e-3831.91Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1[more]
O143531.7e-3230.56Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX... [more]
BLAST of Lcy09g011930 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GYI61.9e-19187.60biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3... [more]
A0A6J1JK174.1e-19187.60biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 S... [more]
A0A1S3BH314.0e-18682.69biotin--protein ligase 2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103489963... [more]
A0A1S3BIK36.4e-18481.01biotin--protein ligase 2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489963... [more]
A0A1S4DWM14.3e-18080.94biotin--protein ligase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489963... [more]
BLAST of Lcy09g011930 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023517017.12.3e-19187.34biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022957207.13.8e-19187.60biotin--protein ligase 2-like [Cucurbita moschata][more]
XP_022990857.18.6e-19187.60biotin--protein ligase 2-like [Cucurbita maxima][more]
KAG6602073.13.7e-18687.73Serine/threonine-protein kinase MPS1, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_038896816.14.9e-18684.50biotin--protein ligase 2-like isoform X1 [Benincasa hispida] >XP_038896824.1 bio... [more]
BLAST of Lcy09g011930 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G25710.15.8e-13764.64holocarboxylase synthase 1 [more]
AT2G25710.25.8e-13764.64holocarboxylase synthase 1 [more]
AT1G37150.24.3e-13265.05holocarboxylase synthetase 2 [more]
AT1G37150.42.0e-11372.40holocarboxylase synthetase 2 [more]
AT1G37150.31.1e-11162.54holocarboxylase synthetase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004408Biotin--acetyl-CoA-carboxylase ligaseTIGRFAMTIGR00121TIGR00121coord: 137..359
e-value: 4.1E-36
score: 122.7
IPR004408Biotin--acetyl-CoA-carboxylase ligaseCDDcd16442BPLcoord: 133..308
e-value: 9.05281E-52
score: 168.981
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPFAMPF03099BPL_LplA_LipBcoord: 138..264
e-value: 2.4E-29
score: 101.9
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPROSITEPS51733BPL_LPL_CATALYTICcoord: 119..309
score: 26.432642
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 121..323
e-value: 1.0E-44
score: 154.2
NoneNo IPR availablePANTHERPTHR12835BIOTIN PROTEIN LIGASEcoord: 61..386
NoneNo IPR availablePANTHERPTHR12835:SF5HOLOCARBOXYLASE SYNTHETASE (BIOTIN-(PROPRIONYL-COA-CARBOXYLASE (ATP-HYDROLYSING)) LIGASE)coord: 61..386
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 119..324
IPR003142Biotin protein ligase, C-terminalPFAMPF02237BPL_Ccoord: 326..381
e-value: 1.7E-8
score: 34.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g011930.1Lcy09g011930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
molecular_function GO:0004077 biotin-[acetyl-CoA-carboxylase] ligase activity