Lcy09g011770 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g011770
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGRAS domain-containing protein
LocationChr09: 12823116 .. 12825036 (+)
RNA-Seq ExpressionLcy09g011770
SyntenyLcy09g011770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTAATCTCTAAAATTAGTATTACTGGTCTTTCTCTTCCACCAGCTAATGCCTACATATAAACCCAACCATTTCTTTTGAATCTTCTCAATCAGAAAAAAAAACTCAATTGCTCTGAAATCCACTGTGTATGATCCAAACTGTAAACTCTCCGATGGCTTTGGCCATCGACAACCCTTTTACCGACCTCGCCCTTTCCGATTACAGCACTTCCACCAACAACTCCGACGACGCCCACCACCTCGCCGGAAACTGGAACTACTTGTCGCCGGTCGTCGGCTGGGAGTCCTTTCCGAGCACCCACGTCGACTTCCACGACCTCTTCGATTCCATGATGGCCGTCGATGCTCCGCCGTATCCACCGCGTGTGGCGGAGGTCGACAGCGGCAGCAACTACAACTCTGGGTCCAGTCCGACGGAGGACCAGGAGGAAGAGGAAGAAGAGGCGAAAGTAGAGGATCGGGAGGAGGAAGAATATAAAGGGCTTCGGCTCTACCACCTCCTCATGGCGGCTGCAGACGCCTTGTTTGGCGACCACAAGAGCCGCGATTTGGCTCATGTGATATTGGTTCGGCTCAATGAATTGGTTTCCCCTTCACACGGGACTAACCTCGAACGCCTCACCGCGTATTACGCTCAAGCTTTTCAGGGCTTGCTCGATTGCGCCGCCGTCTCCGCCGGCGCCGGAGGTTGTTCGAATAAACCCCATCACCATCACAACCACCATCATCTCCACCGCGACGACAATAGTCTGACGGACGTTCTGGCGGCATTTCAATTGCTGCAGGAGATGTCCCCTTATGTGAAATTCAGCCATTTCACTGCAAATCAGGCGATTCTAGAGGCGGTGGTCGATGACCGAAGAGTCCACATAGTGGATTACGATATAATGGAAGGGAGTCAATGGGCGTCGTTGATGCAGGCTTTTGTGTCGAGAAAGGACGGCCCACCGGCCCCACATCTAAGAATCACCGCCATTTCCAGAGGCGGCGGTGGAGCCACTGGCCGGAGATCTATTGCGACGGTTCAGGAGACAGGGCGACGATTGGTGGCGTTTGCGGCTTCGATTGGCCAACCCTTTTCGTTTCATCAGTGTAAATTGGATTCCGATGAGAGTTTTCGTCCTTCTGGGTTGAAATTGGTCAGAGGGGAAGCGCTTGTGGTGAACTGTATGCTCCATCTCCCTCATTTCAGTTACCGTATGCCGGAATCCATCGCTTCTTTTCTCTCCGGCGCCAAGACATTGAATCCGAGGCTGGTTACTTTGGTCGAAGAGGAAATCGGACATGGACCCACCATGGACGGCGATTACAAGGTCCAATTCCTCGATTCCTTGGAGCGTTACTCGGCGATTTATGATTCACTCGGAGCAGGGTTTCCGAAGAAAAACAGAGCAAGGGCATTGGTGGAGAGGGTTTTCCTCGGGCCGAGAATATCAGCCACTCTGACCCGAATCGGACAGCCGCAAGCGACGGCGGCGGAGGAGAATTTCTCATGGGGGGAGAGATTGGAGAAGTTGGGATTCAAGGCGGCGGCGATCAGCTTTGCGAATCACTGTCAAGCGAGACTGTTGCTGGATTTGTTCAACGATGGGTACAGAGTTGAAGAATTGGGCAACAATAAGCTGGTTTTGGGATGGAAATCAAAGCGTTTGCTTTCGACTTCCATTTGGGTTTCTTCATCTTCTTCTTCTTCTTTATGTTCTTCATCGGATTCCGAGTAGAGATGTGTGTGTTTATGGTTGCTGTGTCTTGTTCTTGTGTTCTGATGAATCTGCTCTGTACTGACAGTCTCTGTTTCGTTCTAACATCCACCATTTTTTTCCAGGTTTGGTTGCTGTTTCTTTTCCTCCTTTTGGGGTAAGCCAATTTAAGTAGTTGCTTATTTGAGGAAAGACATGGAGTAAAATAATGAAGGTTT

mRNA sequence

AAATTAATCTCTAAAATTAGTATTACTGGTCTTTCTCTTCCACCAGCTAATGCCTACATATAAACCCAACCATTTCTTTTGAATCTTCTCAATCAGAAAAAAAAACTCAATTGCTCTGAAATCCACTGTGTATGATCCAAACTGTAAACTCTCCGATGGCTTTGGCCATCGACAACCCTTTTACCGACCTCGCCCTTTCCGATTACAGCACTTCCACCAACAACTCCGACGACGCCCACCACCTCGCCGGAAACTGGAACTACTTGTCGCCGGTCGTCGGCTGGGAGTCCTTTCCGAGCACCCACGTCGACTTCCACGACCTCTTCGATTCCATGATGGCCGTCGATGCTCCGCCGTATCCACCGCGTGTGGCGGAGGTCGACAGCGGCAGCAACTACAACTCTGGGTCCAGTCCGACGGAGGACCAGGAGGAAGAGGAAGAAGAGGCGAAAGTAGAGGATCGGGAGGAGGAAGAATATAAAGGGCTTCGGCTCTACCACCTCCTCATGGCGGCTGCAGACGCCTTGTTTGGCGACCACAAGAGCCGCGATTTGGCTCATGTGATATTGGTTCGGCTCAATGAATTGGTTTCCCCTTCACACGGGACTAACCTCGAACGCCTCACCGCGTATTACGCTCAAGCTTTTCAGGGCTTGCTCGATTGCGCCGCCGTCTCCGCCGGCGCCGGAGGTTGTTCGAATAAACCCCATCACCATCACAACCACCATCATCTCCACCGCGACGACAATAGTCTGACGGACGTTCTGGCGGCATTTCAATTGCTGCAGGAGATGTCCCCTTATGTGAAATTCAGCCATTTCACTGCAAATCAGGCGATTCTAGAGGCGGTGGTCGATGACCGAAGAGTCCACATAGTGGATTACGATATAATGGAAGGGAGTCAATGGGCGTCGTTGATGCAGGCTTTTGTGTCGAGAAAGGACGGCCCACCGGCCCCACATCTAAGAATCACCGCCATTTCCAGAGGCGGCGGTGGAGCCACTGGCCGGAGATCTATTGCGACGGTTCAGGAGACAGGGCGACGATTGGTGGCGTTTGCGGCTTCGATTGGCCAACCCTTTTCGTTTCATCAGTGTAAATTGGATTCCGATGAGAGTTTTCGTCCTTCTGGGTTGAAATTGGTCAGAGGGGAAGCGCTTGTGGTGAACTGTATGCTCCATCTCCCTCATTTCAGTTACCGTATGCCGGAATCCATCGCTTCTTTTCTCTCCGGCGCCAAGACATTGAATCCGAGGCTGGTTACTTTGGTCGAAGAGGAAATCGGACATGGACCCACCATGGACGGCGATTACAAGGTCCAATTCCTCGATTCCTTGGAGCGTTACTCGGCGATTTATGATTCACTCGGAGCAGGGTTTCCGAAGAAAAACAGAGCAAGGGCATTGGTGGAGAGGGTTTTCCTCGGGCCGAGAATATCAGCCACTCTGACCCGAATCGGACAGCCGCAAGCGACGGCGGCGGAGGAGAATTTCTCATGGGGGGAGAGATTGGAGAAGTTGGGATTCAAGGCGGCGGCGATCAGCTTTGCGAATCACTGTCAAGCGAGACTGTTGCTGGATTTGTTCAACGATGGGTACAGAGTTGAAGAATTGGGCAACAATAAGCTGGTTTTGGGATGGAAATCAAAGCGTTTGCTTTCGACTTCCATTTGGGTTTCTTCATCTTCTTCTTCTTCTTTATGTTCTTCATCGGATTCCGAGTAGAGATGTTTGGTTGCTGTTTCTTTTCCTCCTTTTGGGGTAAGCCAATTTAAGTAGTTGCTTATTTGAGGAAAGACATGGAGTAAAATAATGAAGGTTT

Coding sequence (CDS)

ATGATCCAAACTGTAAACTCTCCGATGGCTTTGGCCATCGACAACCCTTTTACCGACCTCGCCCTTTCCGATTACAGCACTTCCACCAACAACTCCGACGACGCCCACCACCTCGCCGGAAACTGGAACTACTTGTCGCCGGTCGTCGGCTGGGAGTCCTTTCCGAGCACCCACGTCGACTTCCACGACCTCTTCGATTCCATGATGGCCGTCGATGCTCCGCCGTATCCACCGCGTGTGGCGGAGGTCGACAGCGGCAGCAACTACAACTCTGGGTCCAGTCCGACGGAGGACCAGGAGGAAGAGGAAGAAGAGGCGAAAGTAGAGGATCGGGAGGAGGAAGAATATAAAGGGCTTCGGCTCTACCACCTCCTCATGGCGGCTGCAGACGCCTTGTTTGGCGACCACAAGAGCCGCGATTTGGCTCATGTGATATTGGTTCGGCTCAATGAATTGGTTTCCCCTTCACACGGGACTAACCTCGAACGCCTCACCGCGTATTACGCTCAAGCTTTTCAGGGCTTGCTCGATTGCGCCGCCGTCTCCGCCGGCGCCGGAGGTTGTTCGAATAAACCCCATCACCATCACAACCACCATCATCTCCACCGCGACGACAATAGTCTGACGGACGTTCTGGCGGCATTTCAATTGCTGCAGGAGATGTCCCCTTATGTGAAATTCAGCCATTTCACTGCAAATCAGGCGATTCTAGAGGCGGTGGTCGATGACCGAAGAGTCCACATAGTGGATTACGATATAATGGAAGGGAGTCAATGGGCGTCGTTGATGCAGGCTTTTGTGTCGAGAAAGGACGGCCCACCGGCCCCACATCTAAGAATCACCGCCATTTCCAGAGGCGGCGGTGGAGCCACTGGCCGGAGATCTATTGCGACGGTTCAGGAGACAGGGCGACGATTGGTGGCGTTTGCGGCTTCGATTGGCCAACCCTTTTCGTTTCATCAGTGTAAATTGGATTCCGATGAGAGTTTTCGTCCTTCTGGGTTGAAATTGGTCAGAGGGGAAGCGCTTGTGGTGAACTGTATGCTCCATCTCCCTCATTTCAGTTACCGTATGCCGGAATCCATCGCTTCTTTTCTCTCCGGCGCCAAGACATTGAATCCGAGGCTGGTTACTTTGGTCGAAGAGGAAATCGGACATGGACCCACCATGGACGGCGATTACAAGGTCCAATTCCTCGATTCCTTGGAGCGTTACTCGGCGATTTATGATTCACTCGGAGCAGGGTTTCCGAAGAAAAACAGAGCAAGGGCATTGGTGGAGAGGGTTTTCCTCGGGCCGAGAATATCAGCCACTCTGACCCGAATCGGACAGCCGCAAGCGACGGCGGCGGAGGAGAATTTCTCATGGGGGGAGAGATTGGAGAAGTTGGGATTCAAGGCGGCGGCGATCAGCTTTGCGAATCACTGTCAAGCGAGACTGTTGCTGGATTTGTTCAACGATGGGTACAGAGTTGAAGAATTGGGCAACAATAAGCTGGTTTTGGGATGGAAATCAAAGCGTTTGCTTTCGACTTCCATTTGGGTTTCTTCATCTTCTTCTTCTTCTTTATGTTCTTCATCGGATTCCGAGTAG

Protein sequence

MIQTVNSPMALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSMMAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCSNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE
Homology
BLAST of Lcy09g011770 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.5e-148
Identity = 291/519 (56.07%), Postives = 366/519 (70.52%), Query Frame = 0

Query: 19  DLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSMMAVDAPPYPP 78
           DL  S +S+ TN           WN+ SP+V W++F     DFH L D+++         
Sbjct: 11  DLHFSGHSSLTNTPTSDEDYGCTWNHWSPIVNWDTFTGAPDDFHHLMDTII----EDRTT 70

Query: 79  RVAEVDSGSNYNSGSSPTEDQEEEEEE-------AKVEDRE-EEEYKGLRLYHLLMAAAD 138
            + ++       + ++ T D+EEEE E         ++  E  ++ KGL+L HLLMA A+
Sbjct: 71  VLEQLSPSITTTTTTTTTTDEEEEEMETTTTTTTTAIKTHEVGDDSKGLKLVHLLMAGAE 130

Query: 139 ALFGDHKSRDLAHVILVRLNELVSP-SHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCS 198
           AL G  K+RDLA VIL+RL ELVS  ++G+N+ERL A++ +A  GLL+      GAGG  
Sbjct: 131 ALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLE------GAGGAH 190

Query: 199 NKPHHHHNHHH---LHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRV 258
           N  HHH+N+ H    +   ++  D LAAFQLLQ+MSPYVKF HFTANQAI+EAV  +RRV
Sbjct: 191 NNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRV 250

Query: 259 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRL 318
           H++DYDIMEG QWASL+Q+  S  +G   PHLRITA+SR G   TGRRSIATVQETGRRL
Sbjct: 251 HVIDYDIMEGVQWASLIQSLASNNNG---PHLRITALSRTG---TGRRSIATVQETGRRL 310

Query: 319 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFL 378
            +FAAS+GQPFSFH C+LDSDE+FRPS LKLVRGEALV NCML+LPH SYR PES+ASFL
Sbjct: 311 TSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFL 370

Query: 379 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 438
           +GAKTLNP+LVTLVEEE+G   ++ G +  +F+DSL  YSA++DSL AGFP +NRAR LV
Sbjct: 371 NGAKTLNPKLVTLVEEEVG---SVIGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARTLV 430

Query: 439 ERVFLGPRISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 498
           ERVF GPRI+ +L RI   +    EE  SWGE L ++GF+   +SFANHCQA+LLL LFN
Sbjct: 431 ERVFFGPRIAGSLGRI--YRTGGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFN 490

Query: 499 DGYRVEE--LGNNKLVLGWKSKRLLSTSIWVSSSSSSSL 524
           DGYRVEE  +G+NKLVL WKS+RLLS S+W  SSS S L
Sbjct: 491 DGYRVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSDSDL 508

BLAST of Lcy09g011770 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-145
Identity = 290/515 (56.31%), Postives = 354/515 (68.74%), Query Frame = 0

Query: 20  LALSDYSTSTNN-SDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSMMAVDAPPYPP 79
           L  S +ST TN  S D  +   +WN+ SPVV W++F     DFH L DSM          
Sbjct: 11  LDFSGHSTLTNTPSSDNDNYGCSWNHWSPVVNWDAFTGNQDDFHHLIDSM---------- 70

Query: 80  RVAEVDSGSNYNSGSSPTEDQEEEEEEA-------------KVEDREEEEYKGLRLYHLL 139
            + + ++G  ++  ++ T  +EEEEEEA                +  ++++KGLRL HLL
Sbjct: 71  -IDDNNTGPAFSDHTASTTSEEEEEEEATTTTMTTTTTTTTTTPEAADDDFKGLRLVHLL 130

Query: 140 MAAADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGA 199
           MA A+AL G +K+R+LA VILVRL ELVS + GTN+ERL AY+ +A QGLL+      GA
Sbjct: 131 MAGAEALTGANKNRELARVILVRLKELVSHTDGTNMERLAAYFTEALQGLLE------GA 190

Query: 200 GGCSNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRR 259
           GG  N    HH     H +  +  D LAAFQLLQ+MSPYVKF HFTANQAI+EAV  +RR
Sbjct: 191 GGAYNSSSKHHVIGGPHHEPQN--DALAAFQLLQDMSPYVKFGHFTANQAIVEAVAHERR 250

Query: 260 VHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRR 319
           VHIVDYDIMEG QWASLMQA  S  +G   PHLRITA+SR G    GRRS+ATVQETGRR
Sbjct: 251 VHIVDYDIMEGVQWASLMQALASNPNG---PHLRITALSRSG---VGRRSMATVQETGRR 310

Query: 320 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASF 379
           L AFA S+GQPFSFH  +L+SDE+FRP+GLKLVRGEALV NCML+LPH +YR P S+ASF
Sbjct: 311 LTAFATSLGQPFSFHHSRLESDETFRPAGLKLVRGEALVFNCMLNLPHLTYRSPNSVASF 370

Query: 380 LSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARAL 439
           L+ AK L PRLVT+VEEE+G   +  G +  +F+DSL  +SA++DSL AGFP + RARAL
Sbjct: 371 LTAAKALRPRLVTVVEEEVG---SALGGFVERFMDSLHHFSAVFDSLEAGFPMQGRARAL 430

Query: 440 VERVFLGPRISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLF 499
           VERVFLGPRI  +L RI +      EE  SW E L   GF   A+S ANHCQ+ LLL LF
Sbjct: 431 VERVFLGPRIVGSLARIYR-TGGGGEERGSWREWLRAAGFSGVAVSSANHCQSNLLLGLF 490

Query: 500 NDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSS 521
           NDGYRVEELG+NKLVL WK++RLLS S+W  SS S
Sbjct: 491 NDGYRVEELGSNKLVLHWKTRRLLSASLWTCSSES 496

BLAST of Lcy09g011770 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.2e-115
Identity = 266/556 (47.84%), Postives = 329/556 (59.17%), Query Frame = 0

Query: 11  LAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTH----VDFHDLFD 70
           +A D  F+    +  ++S ++ DD   +   W  LSPV  W +F  +      D H L +
Sbjct: 8   VAGDFEFSGCGSTTTTSSASSLDDGTGMCYAWGELSPVADWANFCCSDDDGGHDLHGLIE 67

Query: 71  SMMAVDAPPYPPRVAEVDSGSN--------------YNSGSSPTEDQEEEEEEAKVEDRE 130
           SM+  D       V   D G                Y +GS+P+            +D  
Sbjct: 68  SMLCDDT-----LVGVDDDGQAGLHHADMFRDDLYCYGNGSNPSSTTTTNPGSPVFDDPT 127

Query: 131 EE-EYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNELVS-----PSHGTNLERLTA 190
           +    KGLRL HLLMAAA+AL G HKSR+LA VILVRL E+VS      +  +N+ERL A
Sbjct: 128 QGCPEKGLRLLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERLAA 187

Query: 191 YYAQAFQGLLDCAAVSAGAGGCSNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVK 250
           ++  A QGLLD    S   GG   +     +HHH         DVL AFQ+LQ+MSPY+K
Sbjct: 188 HFTDALQGLLD---GSHPVGGSGRQAAAAASHHH-------AGDVLTAFQMLQDMSPYMK 247

Query: 251 FSHFTANQAILEAVVDDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRG 310
           F HFTANQAILEAV  DRRVHIVDYDI EG QWASLMQA  SR DG PAPHLRITA+SR 
Sbjct: 248 FGHFTANQAILEAVSGDRRVHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITAVSRS 307

Query: 311 GGGATGRRSIATVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVN 370
           GGG  G R+   VQE GRRL AFAASIGQPFSF QC+LDSDE FRP+ +++V+GEALV N
Sbjct: 308 GGG--GARA---VQEAGRRLSAFAASIGQPFSFGQCRLDSDERFRPATVRMVKGEALVAN 367

Query: 371 CMLH--LPHFSYRMPE-SIASFLSGAKTLNPRLVTLVEEEIGHGPTMDGD---------Y 430
           C+LH      + R P  S+ASFLSG   L  +LVT+VEEE       DGD         +
Sbjct: 368 CVLHQAAATTTIRRPTGSVASFLSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAAAGGF 427

Query: 431 KVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLTRIGQPQATAAEENF 490
             QF++ L RYSA++DSL AGFP ++R R LVERV L P I+  ++R    +    E   
Sbjct: 428 VRQFMEELHRYSAVWDSLEAGFPTQSRVRGLVERVILAPNIAGAVSR--AYRGVDGEGRC 487

Query: 491 SWGERLEKLGFKAAAISFANHCQARLLLDLFNDGYRVEELGNNKLVLGWKSKRLLSTSIW 525
            WG+ +   GF A  +S  NH QARLLL LFNDGY VEE G NK+VLGWK++RL+S S+W
Sbjct: 488 GWGQWMRGSGFTAVPLSCFNHSQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSASVW 541

BLAST of Lcy09g011770 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.2e-113
Identity = 247/507 (48.72%), Postives = 312/507 (61.54%), Query Frame = 0

Query: 16  PFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPV-VGWESFPSTHVDFHDLFDSMMAVDAP 75
           P+ D     +ST T+    A   + N   L+ + + W+       DF D+ +S+M  +  
Sbjct: 4   PYEDFLDLFFSTHTDPLATAASTSSNGYSLNDLDIDWD------CDFRDVIESIMGDEGA 63

Query: 76  PYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAAADALFG 135
              P    V    +     +         +     + + +E KGLRL HLL+AAADA  G
Sbjct: 64  MMEPESEAVPMLHDQEGLCNSASTGLSVADGVSFGEPKTDESKGLRLVHLLVAAADASTG 123

Query: 136 DHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCSNKPHH 195
            +KSR+L  VIL RL +LVSP   TN+ERL A++      LL+  +V      C  +   
Sbjct: 124 ANKSRELTRVILARLKDLVSPGDRTNMERLAAHFTNGLSKLLERDSVL-----CPQQ--- 183

Query: 196 HHNHHHLHRDD-NSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHIVDYDI 255
                  HRDD     DV++AF+LLQ MSPYV F + TA QAILEAV  +RR+HIVDYDI
Sbjct: 184 -------HRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDYDI 243

Query: 256 MEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVAFAASI 315
            EG QWASLMQA VSR  GP A HLRITA+SR      G++S+A VQETGRRL AFA SI
Sbjct: 244 NEGVQWASLMQALVSRNTGPSAQHLRITALSR---ATNGKKSVAAVQETGRRLTAFADSI 303

Query: 316 GQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSGAKTLN 375
           GQPFS+  CKLD++ +F  S LKLVRGEA+V+NCMLHLP FS++ P S+ SFLS AKTLN
Sbjct: 304 GQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSSVISFLSEAKTLN 363

Query: 376 PRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGP 435
           P+LVTLV EE+G        Y+  F+D L ++SAI+DSL AG    N AR  VERVF+GP
Sbjct: 364 PKLVTLVHEEVGLMGNQGFLYR--FMDLLHQFSAIFDSLEAGLSIANPARGFVERVFIGP 423

Query: 436 RISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFNDGYRVEE 495
            ++  LTRI    A   E   SW + LE  GFK   +SF N CQA+LLL LFNDG+RVEE
Sbjct: 424 WVANWLTRITANDA-EVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDGFRVEE 482

Query: 496 LGNNKLVLGWKSKRLLSTSIWVSSSSS 521
           LG N LVLGWKS+RL+S S W S  ++
Sbjct: 484 LGQNGLVLGWKSRRLVSASFWASCQTN 482

BLAST of Lcy09g011770 vs. ExPASy Swiss-Prot
Match: Q9FUZ7 (Protein SCARECROW OS=Zea mays OX=4577 GN=SCR PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.0e-51
Identity = 151/417 (36.21%), Postives = 227/417 (54.44%), Query Frame = 0

Query: 100 EEEEEEAKVEDREEEEYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNELVSPSHGT 159
           +E +EE + + R+EE   GL L  LL+  A+A+  D  + D AH  L+ + EL +P  GT
Sbjct: 281 KERKEEQRRKQRDEE---GLHLLTLLLQCAEAVNAD--NLDDAHQTLLEIAELATP-FGT 340

Query: 160 NLERLTAYYAQAFQGLLDCAAVSAGAGGCSNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQ 219
           + +R+ AY+A+A    L    VS+  G  +  P        LH        V AAFQ+  
Sbjct: 341 STQRVAAYFAEAMSARL----VSSCLGLYAPLPPGSPAAARLH------GRVAAAFQVFN 400

Query: 220 EMSPYVKFSHFTANQAILEAVVDDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLR 279
            +SP+VKFSHFTANQAI EA   + RVHI+D DIM+G QW  L     SR  GP  P +R
Sbjct: 401 GISPFVKFSHFTANQAIQEAFEREERVHIIDLDIMQGLQWPGLFHILASRPGGP--PRVR 460

Query: 280 ITAISRGGGGATGRRSIATVQETGRRLVAFAASIGQPFSFHQCKL-DSDESFRPSGLKLV 339
           +T +           S+  ++ TG+RL  FA ++G PF F  C + +   +  P  L + 
Sbjct: 461 LTGLG---------ASMEALEATGKRLSDFADTLGLPFEF--CAVAEKAGNVDPEKLGVT 520

Query: 340 RGEALVVNCMLHLPHFSYRMPESIASFLSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQF 399
           R EA+ V+    L H  Y +  S ++ L   + L P++VT+VE+++ H     G +  +F
Sbjct: 521 RREAVAVH---WLHHSLYDVTGSDSNTLWLIQRLAPKVVTMVEQDLSH----SGSFLARF 580

Query: 400 LDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLTRIGQPQATAAEENFSWGE 459
           ++++  YSA++DSL A + + +  R +VE+  L   I   L  +G P  T   +  SW E
Sbjct: 581 VEAIHYYSALFDSLDASYGEDSPERHVVEQQLLSREIRNVLA-VGGPARTGDVKFGSWRE 640

Query: 460 RLEKLGFKAAAISFANHCQARLLLDLF-NDGYRVEELGNNKLVLGWKSKRLLSTSIW 515
           +L + GF+AA+++ +   QA LLL +F +DGY + E  N  L LGWK   LL+ S W
Sbjct: 641 KLAQSGFRAASLAGSAAAQASLLLGMFPSDGYTLVE-ENGALKLGWKDLCLLTASAW 659

BLAST of Lcy09g011770 vs. ExPASy TrEMBL
Match: A0A6J1JUC4 (nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC111489805 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 6.9e-213
Identity = 404/524 (77.10%), Postives = 432/524 (82.44%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MA+A+DN F  +A+S+YSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+V EVD G+N NS S+P ED+EE          EEEE+K LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEVDGGTNCNSPSNPAEDREE----------EEEEFKSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGD+KSR+LA VIL+RLNELVS SHGTNLERLTAYYAQAFQ LLDCAAVS   GG 
Sbjct: 121 ADAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVS---GGV 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
            NKP      HHLHRDD+S TDVLAAFQLLQ+MSPYVKF HFTANQAILEAV DDRRVHI
Sbjct: 181 PNKP------HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDY+IMEG QWASLMQAFVSRKD PPAPHLRIT ISR   G + RR I TVQETGRRLVA
Sbjct: 241 VDYNIMEGIQWASLMQAFVSRKDSPPAPHLRITVISR---GPSSRRWIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHFS   PESIASFL+G
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LVE+
Sbjct: 361 AKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEK 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQPQ  ATAAEEN  WGERLEK+G KAAAISFANHCQARLL+DLFN
Sbjct: 421 VFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFN 476

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELG+NKLVLGWKSKRLLS SIW SSSSSSS  SSSDSE
Sbjct: 481 DGYRVEELGSNKLVLGWKSKRLLSVSIWASSSSSSS-SSSSDSE 476

BLAST of Lcy09g011770 vs. ExPASy TrEMBL
Match: A0A5A7V767 (Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00580 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.6e-212
Identity = 400/524 (76.34%), Postives = 429/524 (81.87%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MALAI+NPF D+ALS+YSTSTNNSDD  HLAGNWNY SP+V WE+F  TH DF D+FDS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           + ++ PP+                   TED++           EE+E+KGLRLYHLL+AA
Sbjct: 61  ICIENPPF------------------LTEDRD-----------EEDEFKGLRLYHLLIAA 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGDH+S DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V       
Sbjct: 121 ADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------- 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
           SNK  HHHNHH  HRDD++ TDVLAAFQLLQEMSPYVKF HFTANQAILEAV +DRRVHI
Sbjct: 181 SNKT-HHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDYDIMEG QWASLMQAFVS    P APHLRITAISR   GA GRRSI TVQETGRRLVA
Sbjct: 241 VDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR---GANGRRSIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHFSYR PESIASFLSG
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARALVER
Sbjct: 361 AKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVER 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQ +    A EEN  WGE+LEK+G K   ISFANHCQARLLL LFN
Sbjct: 421 VFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFN 480

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELGNNKLVLGWKSKRLLS SIW SS+SSSS  S SDSE
Sbjct: 481 DGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

BLAST of Lcy09g011770 vs. ExPASy TrEMBL
Match: A0A1S3BE54 (nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.6e-212
Identity = 400/524 (76.34%), Postives = 429/524 (81.87%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MALAI+NPF D+ALS+YSTSTNNSDD  HLAGNWNY SP+V WE+F  TH DF D+FDS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           + ++ PP+                   TED++           EE+E+KGLRLYHLL+AA
Sbjct: 61  ICIENPPF------------------LTEDRD-----------EEDEFKGLRLYHLLIAA 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGDH+S DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V       
Sbjct: 121 ADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------- 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
           SNK  HHHNHH  HRDD++ TDVLAAFQLLQEMSPYVKF HFTANQAILEAV +DRRVHI
Sbjct: 181 SNKT-HHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDYDIMEG QWASLMQAFVS    P APHLRITAISR   GA GRRSI TVQETGRRLVA
Sbjct: 241 VDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR---GANGRRSIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHFSYR PESIASFLSG
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARALVER
Sbjct: 361 AKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVER 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQ +    A EEN  WGE+LEK+G K   ISFANHCQARLLL LFN
Sbjct: 421 VFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFN 480

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELGNNKLVLGWKSKRLLS SIW SS+SSSS  S SDSE
Sbjct: 481 DGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

BLAST of Lcy09g011770 vs. ExPASy TrEMBL
Match: A0A6J1FK48 (nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC111444761 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.9e-210
Identity = 400/524 (76.34%), Postives = 429/524 (81.87%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNS+D                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSND-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+V E+D G+N NS S+P E +EE          EEEE++ LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREE----------EEEEFRSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGDHKSR+LA VIL+RLNELVS SHGTNLER+TAYYAQAFQ LLDCAAVS   GG 
Sbjct: 121 ADAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVS---GGG 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
            NKP      HHLHRDD+S TDVLAAFQLLQEMSPYVKF HFTANQAILEAV DDRRVHI
Sbjct: 181 PNKP------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRLVA
Sbjct: 241 VDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHFS   PESIASFL+G
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LVE+
Sbjct: 361 AKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEK 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RI QPQ  ATAAEEN  WGERLEK+G KAAAISFANHCQARLL+DLFN
Sbjct: 421 VFLGPRISATLRRIEQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFN 476

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 DGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lcy09g011770 vs. ExPASy TrEMBL
Match: A0A0A0KDX4 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 8.7e-208
Identity = 397/524 (75.76%), Postives = 426/524 (81.30%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MALAI+NP  D+ALS+YSTSTNNSDD  HLAGNWNY SP+V WE+F  TH DF D+FDS 
Sbjct: 1   MALAINNPPKDIALSNYSTSTNNSDDHCHLAGNWNYGSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           + ++ PP+                   TED++           EE+E+KGLRLYHLL AA
Sbjct: 61  ICIENPPF------------------LTEDRD-----------EEDEFKGLRLYHLLTAA 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+ GDHKS DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V       
Sbjct: 121 ADAVLGDHKSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------- 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
           +NK  HHHNHH   RDD++ TDVLAAFQLLQEMSPYVKF HFTANQAILEAV +DRRVHI
Sbjct: 181 ANKT-HHHNHHIHQRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDYDIMEG QWASLMQAFVS    P APHLRITAISR   GA GRRSI TVQETGRRLVA
Sbjct: 241 VDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR---GANGRRSIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHFSYR PESIASFLSG
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARALVER
Sbjct: 361 AKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVER 420

Query: 429 VFLGPRISATLTRIGQPQATAA--EENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQ + + A  E+N  WGE+LEK+G K   ISFANHCQARLLL LFN
Sbjct: 421 VFLGPRISATLRRIGQRRWSTAEMEDNCLWGEKLEKMGLKTVKISFANHCQARLLLGLFN 478

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELGNNKLVLGWKSKRLLS SIW SSSSSSSL   SDSE
Sbjct: 481 DGYRVEELGNNKLVLGWKSKRLLSVSIWGSSSSSSSL---SDSE 478

BLAST of Lcy09g011770 vs. NCBI nr
Match: XP_038884439.1 (protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida])

HSP 1 Score: 767.3 bits (1980), Expect = 8.6e-218
Identity = 411/531 (77.40%), Postives = 439/531 (82.67%), Query Frame = 0

Query: 2   IQTVNSPMALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDF 61
           I ++NSPMALA+DN F ++ LS+YSTSTNNSDD  HLAGNWNY SP+V WE+FP TH DF
Sbjct: 16  INSLNSPMALAMDNLFNEITLSNYSTSTNNSDDHRHLAGNWNYGSPIVDWETFPDTHNDF 75

Query: 62  HDLFDSMMAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRL 121
           HDL DSM+ +D PP                  SPTED+E           EEE++KGLRL
Sbjct: 76  HDLVDSMICIDNPP------------------SPTEDRE-----------EEEDFKGLRL 135

Query: 122 YHLLMAAADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAV 181
           YHLL+AAADA+FGDHKSRDLAHVILVRLNELVSPSHGTNL+RLTAYYAQAFQ LLD   V
Sbjct: 136 YHLLVAAADAVFGDHKSRDLAHVILVRLNELVSPSHGTNLQRLTAYYAQAFQDLLDSTPV 195

Query: 182 SAGAGGCSNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVV 241
                  SNK HHH  +HHLHRDD++ TDVLAAFQLLQEMSPYVKFSHFTANQAILEAV 
Sbjct: 196 -------SNKTHHH--NHHLHRDDHTPTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVA 255

Query: 242 DDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQE 301
           +DRRVHIVDYDIMEG QWASLMQAFVS    P APHLRITAISR   GA GRRSI TVQE
Sbjct: 256 EDRRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR---GANGRRSIGTVQE 315

Query: 302 TGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPES 361
           TGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNC+LHLPHFSYR PES
Sbjct: 316 TGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCILHLPHFSYRSPES 375

Query: 362 IASFLSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNR 421
           I SFLSG K+LNPR+VTLVEEEIGHGPT+D DYKVQFLDSLERYSAIYDSL A  P KNR
Sbjct: 376 IGSFLSGTKSLNPRIVTLVEEEIGHGPTIDVDYKVQFLDSLERYSAIYDSLEAVIPMKNR 435

Query: 422 ARALVERVFLGPRISATLTRIGQPQ-ATA-AEENFSWGERLEKLGFKAAAISFANHCQAR 481
           ARALVERVFLGPRISATL RIGQ + ATA AEEN  WGE+LEK+G K A ISFANHCQAR
Sbjct: 436 ARALVERVFLGPRISATLRRIGQRRWATAGAEENCLWGEKLEKMGLKPATISFANHCQAR 495

Query: 482 LLLDLFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           LLL LFNDGYRVEELGNNKLVLGWKSKRLLS SIWVSSS SSS  S SDSE
Sbjct: 496 LLLGLFNDGYRVEELGNNKLVLGWKSKRLLSASIWVSSSLSSSSSSLSDSE 502

BLAST of Lcy09g011770 vs. NCBI nr
Match: XP_022993952.1 (nodulation-signaling pathway 2 protein [Cucurbita maxima])

HSP 1 Score: 750.0 bits (1935), Expect = 1.4e-212
Identity = 404/524 (77.10%), Postives = 432/524 (82.44%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MA+A+DN F  +A+S+YSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+V EVD G+N NS S+P ED+EE          EEEE+K LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEVDGGTNCNSPSNPAEDREE----------EEEEFKSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGD+KSR+LA VIL+RLNELVS SHGTNLERLTAYYAQAFQ LLDCAAVS   GG 
Sbjct: 121 ADAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVS---GGV 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
            NKP      HHLHRDD+S TDVLAAFQLLQ+MSPYVKF HFTANQAILEAV DDRRVHI
Sbjct: 181 PNKP------HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDY+IMEG QWASLMQAFVSRKD PPAPHLRIT ISR   G + RR I TVQETGRRLVA
Sbjct: 241 VDYNIMEGIQWASLMQAFVSRKDSPPAPHLRITVISR---GPSSRRWIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHFS   PESIASFL+G
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LVE+
Sbjct: 361 AKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEK 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQPQ  ATAAEEN  WGERLEK+G KAAAISFANHCQARLL+DLFN
Sbjct: 421 VFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFN 476

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELG+NKLVLGWKSKRLLS SIW SSSSSSS  SSSDSE
Sbjct: 481 DGYRVEELGSNKLVLGWKSKRLLSVSIWASSSSSSS-SSSSDSE 476

BLAST of Lcy09g011770 vs. NCBI nr
Match: XP_023551167.1 (nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 750.0 bits (1935), Expect = 1.4e-212
Identity = 406/524 (77.48%), Postives = 431/524 (82.25%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+V EVD G+N NS S+P ED+EE          EEEE+K LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEVDGGTNCNSPSNPAEDREE----------EEEEFKSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGDHKSRDLA VIL+RLNELVS SHGTNLERLTAYYAQ+FQ LLD AAVS   GG 
Sbjct: 121 ADAMFGDHKSRDLAQVILIRLNELVSTSHGTNLERLTAYYAQSFQDLLDYAAVS---GGG 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
           SNKP      HHLHRDD+S TDVLAAFQLLQEMSPYVKF HFTANQAILEAV DDRRVHI
Sbjct: 181 SNKP------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRLVA
Sbjct: 241 VDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHFS   PESI SFL+G
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIDSFLAG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LVE+
Sbjct: 361 AKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEK 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQPQ  ATAAEEN SWGERLEK+G KAAAISFANHCQARLL+DLFN
Sbjct: 421 VFLGPRISATLKRIGQPQRAATAAEENCSWGERLEKMGLKAAAISFANHCQARLLVDLFN 476

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 DGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lcy09g011770 vs. NCBI nr
Match: XP_008445682.1 (PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa] >TYK05186.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 748.0 bits (1930), Expect = 5.4e-212
Identity = 400/524 (76.34%), Postives = 429/524 (81.87%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MALAI+NPF D+ALS+YSTSTNNSDD  HLAGNWNY SP+V WE+F  TH DF D+FDS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           + ++ PP+                   TED++           EE+E+KGLRLYHLL+AA
Sbjct: 61  ICIENPPF------------------LTEDRD-----------EEDEFKGLRLYHLLIAA 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGDH+S DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V       
Sbjct: 121 ADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------- 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
           SNK  HHHNHH  HRDD++ TDVLAAFQLLQEMSPYVKF HFTANQAILEAV +DRRVHI
Sbjct: 181 SNKT-HHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDYDIMEG QWASLMQAFVS    P APHLRITAISR   GA GRRSI TVQETGRRLVA
Sbjct: 241 VDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR---GANGRRSIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHFSYR PESIASFLSG
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARALVER
Sbjct: 361 AKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVER 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQ +    A EEN  WGE+LEK+G K   ISFANHCQARLLL LFN
Sbjct: 421 VFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFN 480

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELGNNKLVLGWKSKRLLS SIW SS+SSSS  S SDSE
Sbjct: 481 DGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

BLAST of Lcy09g011770 vs. NCBI nr
Match: KAG6578977.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 747.7 bits (1929), Expect = 7.1e-212
Identity = 403/524 (76.91%), Postives = 430/524 (82.06%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVGWESFPSTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+V E+D G+N NS S+P E +EE          EEEE++ LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREE----------EEEEFRSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGC 188
           ADA+FGDHKSR+LA VIL+RLNELVS SHGTNLERLTAYYAQAFQ LLDCAAVS   GG 
Sbjct: 121 ADAMFGDHKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVS---GGG 180

Query: 189 SNKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHI 248
            NKP      HHLHRDD+S TDVLAAFQLLQEMSPYVKF HFTANQAILEAV DDRRVHI
Sbjct: 181 PNKP------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHI 240

Query: 249 VDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVA 308
           VDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRLVA
Sbjct: 241 VDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRLVA 300

Query: 309 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 368
           FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHFS   PESIASFL+G
Sbjct: 301 FAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAG 360

Query: 369 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 428
           AKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LVE+
Sbjct: 361 AKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEK 420

Query: 429 VFLGPRISATLTRIGQPQ--ATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFN 488
           VFLGPRISATL RIGQPQ  ATAAEEN  WGERLEK+G KAAAISFANHCQARLL+DLFN
Sbjct: 421 VFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFN 476

Query: 489 DGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSSSSSLCSSSDSE 531
           DGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 DGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lcy09g011770 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 411.8 bits (1057), Expect = 8.5e-115
Identity = 247/507 (48.72%), Postives = 312/507 (61.54%), Query Frame = 0

Query: 16  PFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPV-VGWESFPSTHVDFHDLFDSMMAVDAP 75
           P+ D     +ST T+    A   + N   L+ + + W+       DF D+ +S+M  +  
Sbjct: 4   PYEDFLDLFFSTHTDPLATAASTSSNGYSLNDLDIDWD------CDFRDVIESIMGDEGA 63

Query: 76  PYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAAADALFG 135
              P    V    +     +         +     + + +E KGLRL HLL+AAADA  G
Sbjct: 64  MMEPESEAVPMLHDQEGLCNSASTGLSVADGVSFGEPKTDESKGLRLVHLLVAAADASTG 123

Query: 136 DHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCSNKPHH 195
            +KSR+L  VIL RL +LVSP   TN+ERL A++      LL+  +V      C  +   
Sbjct: 124 ANKSRELTRVILARLKDLVSPGDRTNMERLAAHFTNGLSKLLERDSVL-----CPQQ--- 183

Query: 196 HHNHHHLHRDD-NSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHIVDYDI 255
                  HRDD     DV++AF+LLQ MSPYV F + TA QAILEAV  +RR+HIVDYDI
Sbjct: 184 -------HRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDYDI 243

Query: 256 MEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVAFAASI 315
            EG QWASLMQA VSR  GP A HLRITA+SR      G++S+A VQETGRRL AFA SI
Sbjct: 244 NEGVQWASLMQALVSRNTGPSAQHLRITALSR---ATNGKKSVAAVQETGRRLTAFADSI 303

Query: 316 GQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSGAKTLN 375
           GQPFS+  CKLD++ +F  S LKLVRGEA+V+NCMLHLP FS++ P S+ SFLS AKTLN
Sbjct: 304 GQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSSVISFLSEAKTLN 363

Query: 376 PRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGP 435
           P+LVTLV EE+G        Y+  F+D L ++SAI+DSL AG    N AR  VERVF+GP
Sbjct: 364 PKLVTLVHEEVGLMGNQGFLYR--FMDLLHQFSAIFDSLEAGLSIANPARGFVERVFIGP 423

Query: 436 RISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLFNDGYRVEE 495
            ++  LTRI    A   E   SW + LE  GFK   +SF N CQA+LLL LFNDG+RVEE
Sbjct: 424 WVANWLTRITANDA-EVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDGFRVEE 482

Query: 496 LGNNKLVLGWKSKRLLSTSIWVSSSSS 521
           LG N LVLGWKS+RL+S S W S  ++
Sbjct: 484 LGQNGLVLGWKSRRLVSASFWASCQTN 482

BLAST of Lcy09g011770 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 201.1 bits (510), Expect = 2.3e-51
Identity = 148/451 (32.82%), Postives = 235/451 (52.11%), Query Frame = 0

Query: 70  AVDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAAA 129
           + DAPP P  V         N+     E   E +EE K   R++++ +GL L  LL+  A
Sbjct: 246 STDAPPQPETVTATVPAVQTNT----AEALRERKEEIK---RQKQDEEGLHLLTLLLQCA 305

Query: 130 DALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCS 189
           +A+  D+     A+ +L+ +++L +P +GT+ +R+ AY+++A    L  + +   A   S
Sbjct: 306 EAVSADNLEE--ANKLLLEISQLSTP-YGTSAQRVAAYFSEAMSARLLNSCLGIYAALPS 365

Query: 190 NKPHHHHNHHHLHRDDNSLTDVLAAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHIV 249
                 H+             +++AFQ+   +SP VKFSHFTANQAI EA   +  VHI+
Sbjct: 366 RWMPQTHS-----------LKMVSAFQVFNGISPLVKFSHFTANQAIQEAFEKEDSVHII 425

Query: 250 DYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQETGRRLVAF 309
           D DIM+G QW  L     SR  GP  PH+R+T +           S+  +Q TG+RL  F
Sbjct: 426 DLDIMQGLQWPGLFHILASRPGGP--PHVRLTGLG---------TSMEALQATGKRLSDF 485

Query: 310 AASIGQPFSFHQCKL-DSDESFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSG 369
           A  +G PF F  C L +   +     L + + EA+ V+    L H  Y +  S A  L  
Sbjct: 486 ADKLGLPFEF--CPLAEKVGNLDTERLNVRKREAVAVH---WLQHSLYDVTGSDAHTLWL 545

Query: 370 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 429
            + L P++VT+VE+++ H     G +  +F++++  YSA++DSLGA + +++  R +VE+
Sbjct: 546 LQRLAPKVVTVVEQDLSHA----GSFLGRFVEAIHYYSALFDSLGASYGEESEERHVVEQ 605

Query: 430 VFLGPRISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLF-ND 489
             L   I   L  +G P  +   +  SW E++++ GFK  +++     QA LLL +F +D
Sbjct: 606 QLLSKEIRNVLA-VGGPSRSGEVKFESWREKMQQCGFKGISLAGNAATQATLLLGMFPSD 653

Query: 490 GYRVEELGNNKLVLGWKSKRLLSTSIWVSSS 519
           GY + +  N  L LGWK   LL+ S W   S
Sbjct: 666 GYTLVD-DNGTLKLGWKDLSLLTASAWTPRS 653

BLAST of Lcy09g011770 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-43
Identity = 133/425 (31.29%), Postives = 205/425 (48.24%), Query Frame = 0

Query: 93  SSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNEL 152
           SS      +   E   E  E +    ++L  LL+  A+ +  DH     A  +L  ++E+
Sbjct: 12  SSDDPSSAKRRIEFPEETLENDGAAAIKLLSLLLQCAEYVATDHLRE--ASTLLSEISEI 71

Query: 153 VSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCSNKPHHHHNHHHLHRDDNSLTDVL 212
            SP  G++ ER+ AY+AQA Q  +  + +S      S KP                  + 
Sbjct: 72  CSP-FGSSPERVVAYFAQALQTRVISSYLSGACSPLSEKP----------LTVVQSQKIF 131

Query: 213 AAFQLLQEMSPYVKFSHFTANQAILEAVVDDRRVHIVDYDIMEGSQWASLMQAFVSRKDG 272
           +A Q    +SP +KFSHFTANQAI +A+  +  VHI+D D+M+G QW +L     SR   
Sbjct: 132 SALQTYNSVSPLIKFSHFTANQAIFQALDGEDSVHIIDLDVMQGLQWPALFHILASRP-- 191

Query: 273 PPAPHLRITAISRGGGGATGRRSIATVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRP 332
                 ++ +I   G G+    S   +  TGRRL  FA+S+  PF FH  +        P
Sbjct: 192 -----RKLRSIRITGFGS----SSDLLASTGRRLADFASSLNLPFEFHPIEGIIGNLIDP 251

Query: 333 SGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSGAKTLNPRLVTLVEEEIGHGPTMDG 392
           S L   +GEA+VV+ M    H  Y +  +    L   + L P L+T+VE+E+ +     G
Sbjct: 252 SQLATRQGEAVVVHWM---QHRLYDVTGNNLETLEILRRLKPNLITVVEQELSYDD--GG 311

Query: 393 DYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLTRIGQPQATAAEE 452
            +  +F+++L  YSA++D+LG G  +++  R  VE++ LG  I   +   G        +
Sbjct: 312 SFLGRFVEALHYYSALFDALGDGLGEESGERFTVEQIVLGTEIRNIVAHGG-----GRRK 371

Query: 453 NFSWGERLEKLGFKAAAISFANHCQARLLLDLFN-DGYRVEELGNNKLVLGWKSKRLLST 512
              W E L ++GF+  ++      QA LLL +   +GY + E  N  L LGWK   LL+ 
Sbjct: 372 RMKWKEELSRVGFRPVSLRGNPATQAGLLLGMLPWNGYTLVE-ENGTLRLGWKDLSLLTA 401

Query: 513 SIWVS 517
           S W S
Sbjct: 432 SAWKS 401

BLAST of Lcy09g011770 vs. TAIR 10
Match: AT1G55580.1 (GRAS family transcription factor )

HSP 1 Score: 174.9 bits (442), Expect = 1.8e-43
Identity = 112/327 (34.25%), Postives = 164/327 (50.15%), Query Frame = 0

Query: 201 LHRDDNSLTDVLAAFQL-LQEMSPYVKFSHFTANQAILEAVV--DDRRVHIVDYDIMEGS 260
           L R  N+ +D  + + L L +++P+++F H TANQAIL+A    D+  +HI+D DI +G 
Sbjct: 131 LFRTKNNNSDFESCYYLWLNQLTPFIRFGHLTANQAILDATETNDNGALHILDLDISQGL 190

Query: 261 QWASLMQAFVSRKDGP--PAPHLRITAISRGGGGATGRRSIATVQETGRRLVAFAASIGQ 320
           QW  LMQA   R   P  P P LRIT            R +  +  TG RL  FA S+G 
Sbjct: 191 QWPPLMQALAERSSNPSSPPPSLRITGCG---------RDVTGLNRTGDRLTRFADSLGL 250

Query: 321 PFSFHQCKLDSDE------SFRPSGLKLVRGEALVVNCMLHLPHFSYRMPESIASFLSGA 380
            F FH   +  ++        R   L  V+GE + VNC+  L        + I  FLS  
Sbjct: 251 QFQFHTLVIVEEDLAGLLLQIRLLALSAVQGETIAVNCVHFLHKIFNDDGDMIGHFLSAI 310

Query: 381 KTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERV 440
           K+LN R+VT+ E E  HG   D  +  +F ++++ Y AI+DSL A  P  +R R  +E+ 
Sbjct: 311 KSLNSRIVTMAEREANHG---DHSFLNRFSEAVDHYMAIFDSLEATLPPNSRERLTLEQR 370

Query: 441 FLGPRISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARLLLDLF--ND 500
           + G  I   +      +         W E +++ GF    I      QA+LLL L   ++
Sbjct: 371 WFGKEILDVVAAEETERKQRHRRFEIWEEMMKRFGFVNVPIGSFALSQAKLLLRLHYPSE 430

Query: 501 GYRVEELGNNKLVLGWKSKRLLSTSIW 515
           GY ++ L NN L LGW+++ L S S W
Sbjct: 431 GYNLQFL-NNSLFLGWQNRPLFSVSSW 444

BLAST of Lcy09g011770 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 173.3 bits (438), Expect = 5.1e-43
Identity = 139/456 (30.48%), Postives = 218/456 (47.81%), Query Frame = 0

Query: 71  VDAPPYPPRVAEVDSGSNYNSGSSPTEDQEEEEEEAKVEDREEEEYKGLRLYHLLMAAAD 130
           + A P    V + ++ S      S  E  +E      + D +E    G+RL H L+A A+
Sbjct: 133 LSAFPKEEEVFDEEASSKRIRLGSWCESSDESTRSVVLVDSQE---TGVRLVHALVACAE 192

Query: 131 ALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSAGAGGCSN 190
           A+    ++ +LA  ++ R+  L     G  + ++  Y+AQA                   
Sbjct: 193 AI--HQENLNLADALVKRVGTLAGSQAGA-MGKVATYFAQALA----------------- 252

Query: 191 KPHHHHNHHHLHRDDNSLTDVLAA----FQLLQEM-----SPYVKFSHFTANQAILEAVV 250
                     ++RD  + TDV AA    F+ + EM      PY+KF+HFTANQAILEAV 
Sbjct: 253 --------RRIYRDYTAETDVCAAVNPSFEEVLEMHFYESCPYLKFAHFTANQAILEAVT 312

Query: 251 DDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATGRRSIATVQE 310
             RRVH++D  + +G QW +LMQA   R  GPP+   R+T I     G     +  ++Q+
Sbjct: 313 TARRVHVIDLGLNQGMQWPALMQALALRPGGPPS--FRLTGI-----GPPQTENSDSLQQ 372

Query: 311 TGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKL-VRGEALVVNCMLHLPHFSYRMPE 370
            G +L  FA ++G  F F     +S     P   +     E LVVN +  L H       
Sbjct: 373 LGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESETLVVNSVFEL-HRLLARSG 432

Query: 371 SIASFLSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKN 430
           SI   L+  K + P +VT+VE+E  H   +  D   +F ++L  YS+++DSL   +   +
Sbjct: 433 SIEKLLNTVKAIKPSIVTVVEQEANHNGIVFLD---RFNEALHYYSSLFDSLEDSYSLPS 492

Query: 431 RARALVERVFLGPRISATLTRIGQPQATAAEENFSWGERLEKLGFKAAAISFANHCQARL 490
           + R + E V+LG +I   +   G  +    E    W  R++  GF    +  +   QA +
Sbjct: 493 QDRVMSE-VYLGRQILNVVAAEGSDRVERHETAAQWRIRMKSAGFDPIHLGSSAFKQASM 544

Query: 491 LLDLF--NDGYRVEELGNNKLVLGWKSKRLLSTSIW 515
           LL L+   DGYRVEE  +  L++GW+++ L++TS W
Sbjct: 553 LLSLYATGDGYRVEE-NDGCLMIGWQTRPLITTSAW 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5NE247.5e-14856.07Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE... [more]
Q2PEG71.6e-14556.31Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 ... [more]
Q84Q922.2e-11547.84Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SUF51.2e-11348.72Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1[more]
Q9FUZ71.0e-5136.21Protein SCARECROW OS=Zea mays OX=4577 GN=SCR PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JUC46.9e-21377.10nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC1114898... [more]
A0A5A7V7672.6e-21276.34Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BE542.6e-21276.34nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 P... [more]
A0A6J1FK481.9e-21076.34nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A0A0KDX48.7e-20875.76GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_038884439.18.6e-21877.40protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida][more]
XP_022993952.11.4e-21277.10nodulation-signaling pathway 2 protein [Cucurbita maxima][more]
XP_023551167.11.4e-21277.48nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_008445682.15.4e-21276.34PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 n... [more]
KAG6578977.17.1e-21276.91Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
AT4G08250.18.5e-11548.72GRAS family transcription factor [more]
AT3G54220.12.3e-5132.82GRAS family transcription factor [more]
AT5G41920.11.3e-4331.29GRAS family transcription factor [more]
AT1G55580.11.8e-4334.25GRAS family transcription factor [more]
AT3G03450.15.1e-4330.48RGA-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005202Transcription factor GRASPFAMPF03514GRAScoord: 122..514
e-value: 9.7E-94
score: 314.4
IPR005202Transcription factor GRASPANTHERPTHR31636OSJNBA0084A10.13 PROTEIN-RELATEDcoord: 24..516
IPR005202Transcription factor GRASPROSITEPS50985GRAScoord: 112..515
score: 69.164062
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..113
IPR030028Scarecrow-like protein 26/nodulation signalling pathway 2PANTHERPTHR31636:SF25SCARECROW-LIKE PROTEIN 26coord: 24..516

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g011770.1Lcy09g011770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding