Lcy09g011030 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g011030
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionWall-associated receptor kinase 2-like
LocationChr09: 11791914 .. 11793615 (-)
RNA-Seq ExpressionLcy09g011030
SyntenyLcy09g011030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGACGTTCGACAAAGACGACGCTCGTACCACTCACGGTGATGAAGATAGCCATGTTGTCAACAGCCTTGGCCGTAGTAGCTTCTCAAGCCTTACCCGGCTGCGACGAATGGTGTGGCGACGTGCAAATTCCATATCCATTCGGCACGAGAAATGGGTGTTATCTCAATGAAACTTTCTCAATTACTTGCAACAAAACCGATGGCAATAAACCTCCAAAGGAGTTTCTAAAGAACACCAACATCAGCGTTACCAATATATCTATGGATGGTGAGCTCCACATCTTGCAGCCCATAGTGCGAGATTGTCACAAGCCAGTCGCTGGTCCTTTCGTTCCCAACCTAACCAATCTTTCGGTGCCGGCCATGTTTCCAATTGCTGATGGCAAAAACAAGTTCAGGACCATCGGCTGCGACGCTTTGGGTTTAGTCCTCGGGGTGCACAACGGGAGTGATTATGCAAGTGGGTGTATTTCGATGTGTTTCAACAATAGTATTATAAATAATGGGACGTGCTCTGGCAATGGGTGCTGTCAGTTGGAGATTCCAAATGGGTTGAGGGATTTGACTTTGCAGGTGATTACCGTCTTGAATTACACTCGTGCACCAAATTTCAGTTCCTGTGGGTATGCTTTTGTAACCGGAGATGAGGGGTTCGAGTTTTCTCGAACTGATATTGAGAATTTTGAAGATGAGGAAGTTGAGGTTGTGCTTGGTTGGGCCATTGGAAAAGAAACAAAAAATGTATGTGGATCAAACAACACATGGAATAGTAGCTTCTCTGATGATGGATCAGAATATTATCGTTGCCAATGTTTGGATGGTTTCGAGGGGAATCCATATCTCCCTCAAGGATGCCAAGGTAAAGGCTTATTCATCTCTCTACTTGGGAATCTATTATATTTTACACACCACTTTATTTATTTATATATATCAATCTGTACCTACCCTGAAGTAATTCAATTAATTTCATGATCTCTTTCAACTTTCAATTAAGTTAAACTAAATCTTTGACTTAGAGAAGGATGATTTTAAAACAAGTGTTATTACTTTGGTGAAAAGTGGTTGATAATAAATCATTTTAATGATTTTTGGCTTCTCAATTATAAATGTGGTTTTGATATACTTAAATATTATCAATGTTTTTGTTTTCATAACTGATTTCAAAACCTTTAATTTACTAAAAACAGTCACTATATTTGAGAGAAAATAAAAGTAAAATGAGTGAAAGACAAAAATTATATGAAGAGGAAAATTTGTTAGAAAAAAAAAATTAGAAAAAGAAGTAAGTCATTGAGACTTGAGAGGGACTAAAATGTTAAAAAGAGAAAAAAAAATGTTAAATTGAAATTAATACAGAGAAAAGTTGATTATTTTCCCTAAAAAAAAAAAAGAAAAAGAAACAGAATCTAGATTGGGGACTTGGGAGAAGTAAATAGGGAAATGTAAACAATTTGGGTTTGTAAGTTTAATTGTTGAATGTTTTGGACTTGTTTTAGATATGGATGAATGCAAGGATGGAAGGCGAAATGATTGTATATACAAGAACAAGTGTGTTAACACAATAGGAAACTATACCTGCAATTGTCCTAACAACTTTAAAGGAGATGGAAGACATTGGAGACATGGGGGACATGAGGGGGAAGGTTGCATCCGAGATTCCTCCTCTCTCATTCTTATCATCATCGGTGAGTTTTTATAA

mRNA sequence

ATGAGACGTTCGACAAAGACGACGCTCGTACCACTCACGGTGATGAAGATAGCCATGTTGTCAACAGCCTTGGCCGTAGTAGCTTCTCAAGCCTTACCCGGCTGCGACGAATGGTGTGGCGACGTGCAAATTCCATATCCATTCGGCACGAGAAATGGGTGTTATCTCAATGAAACTTTCTCAATTACTTGCAACAAAACCGATGGCAATAAACCTCCAAAGGAGTTTCTAAAGAACACCAACATCAGCGTTACCAATATATCTATGGATGGTGAGCTCCACATCTTGCAGCCCATAGTGCGAGATTGTCACAAGCCAGTCGCTGGTCCTTTCGTTCCCAACCTAACCAATCTTTCGGTGCCGGCCATGTTTCCAATTGCTGATGGCAAAAACAAGTTCAGGACCATCGGCTGCGACGCTTTGGGTTTAGTCCTCGGGGTGCACAACGGGAGTGATTATGCAAGTGGGTGTATTTCGATGTGTTTCAACAATAGTATTATAAATAATGGGACGTGCTCTGGCAATGGGTGCTGTCAGTTGGAGATTCCAAATGGGTTGAGGGATTTGACTTTGCAGGTGATTACCGTCTTGAATTACACTCGTGCACCAAATTTCAGTTCCTGTGGGTATGCTTTTGTAACCGGAGATGAGGGGTTCGAGTTTTCTCGAACTGATATTGAGAATTTTGAAGATGAGGAAGTTGAGGTTGTGCTTGGTTGGGCCATTGGAAAAGAAACAAAAAATGTATGTGGATCAAACAACACATGGAATAGTAGCTTCTCTGATGATGGATCAGAATATTATCGTTGCCAATGTTTGGATGGTTTCGAGGGGAATCCATATCTCCCTCAAGGATGCCAAGATATGGATGAATGCAAGGATGGAAGGCGAAATGATTGTATATACAAGAACAAGTGTGTTAACACAATAGGAAACTATACCTGCAATTGTCCTAACAACTTTAAAGGAGATGGAAGACATTGGAGACATGGGGGACATGAGGGGGAAGGTTGCATCCGAGATTCCTCCTCTCTCATTCTTATCATCATCGGTGAGTTTTTATAA

Coding sequence (CDS)

ATGAGACGTTCGACAAAGACGACGCTCGTACCACTCACGGTGATGAAGATAGCCATGTTGTCAACAGCCTTGGCCGTAGTAGCTTCTCAAGCCTTACCCGGCTGCGACGAATGGTGTGGCGACGTGCAAATTCCATATCCATTCGGCACGAGAAATGGGTGTTATCTCAATGAAACTTTCTCAATTACTTGCAACAAAACCGATGGCAATAAACCTCCAAAGGAGTTTCTAAAGAACACCAACATCAGCGTTACCAATATATCTATGGATGGTGAGCTCCACATCTTGCAGCCCATAGTGCGAGATTGTCACAAGCCAGTCGCTGGTCCTTTCGTTCCCAACCTAACCAATCTTTCGGTGCCGGCCATGTTTCCAATTGCTGATGGCAAAAACAAGTTCAGGACCATCGGCTGCGACGCTTTGGGTTTAGTCCTCGGGGTGCACAACGGGAGTGATTATGCAAGTGGGTGTATTTCGATGTGTTTCAACAATAGTATTATAAATAATGGGACGTGCTCTGGCAATGGGTGCTGTCAGTTGGAGATTCCAAATGGGTTGAGGGATTTGACTTTGCAGGTGATTACCGTCTTGAATTACACTCGTGCACCAAATTTCAGTTCCTGTGGGTATGCTTTTGTAACCGGAGATGAGGGGTTCGAGTTTTCTCGAACTGATATTGAGAATTTTGAAGATGAGGAAGTTGAGGTTGTGCTTGGTTGGGCCATTGGAAAAGAAACAAAAAATGTATGTGGATCAAACAACACATGGAATAGTAGCTTCTCTGATGATGGATCAGAATATTATCGTTGCCAATGTTTGGATGGTTTCGAGGGGAATCCATATCTCCCTCAAGGATGCCAAGATATGGATGAATGCAAGGATGGAAGGCGAAATGATTGTATATACAAGAACAAGTGTGTTAACACAATAGGAAACTATACCTGCAATTGTCCTAACAACTTTAAAGGAGATGGAAGACATTGGAGACATGGGGGACATGAGGGGGAAGGTTGCATCCGAGATTCCTCCTCTCTCATTCTTATCATCATCGGTGAGTTTTTATAA

Protein sequence

MRRSTKTTLVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTDGNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIADGKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVNTIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIGEFL
Homology
BLAST of Lcy09g011030 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.0e-47
Identity = 109/309 (35.28%), Postives = 167/309 (54.05%), Query Frame = 0

Query: 26  VVASQALPGCDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDGNKPPKEFLKNTNIS 85
           +V  Q    C   CG+V + YPFGT  GCY   +E+F++TCN+       +E L   N+ 
Sbjct: 20  LVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNE-------QEKLFFGNMP 79

Query: 86  VTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIADGKNKFRTIGCDALGL 145
           V N+S+ G+L +     R C+    G     +   +    F +++  N+F  +GC++   
Sbjct: 80  VINMSLSGQLRVRLVRSRVCYDS-QGKQTDYIAQRTTLGNFTLSE-LNRFTVVGCNSYAF 139

Query: 146 VLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQVITVLNYTRAP 205
            L       Y++GCIS+C +++   NG+CSG GCCQ+ +P G   + ++  +  N+    
Sbjct: 140 -LRTSGVEKYSTGCISIC-DSATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVH 199

Query: 206 NFSSCGYAFVTGDEGFEF-SRTDIENFED-EEVEVVLGWAIGKET------KNVCGSNNT 265
            F+ C YAF+  D  F+F +  D+ N  +     VVL W+IG +T      + VCG N+T
Sbjct: 200 LFNPCTYAFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNST 259

Query: 266 WNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVNTIGNYTC 325
               F   G   Y C+CL+GFEGNPYLP GCQD++EC   R N C   + C NT G++ C
Sbjct: 260 ---CFDSTGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHN-CSEHSTCENTKGSFNC 313

BLAST of Lcy09g011030 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.2e-38
Identity = 111/328 (33.84%), Postives = 159/328 (48.48%), Query Frame = 0

Query: 12  LTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDG 71
           L +M I        +V +Q    C   CGDV I YPFG   GCY   +++F+ITC +   
Sbjct: 6   LFLMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKP 65

Query: 72  NKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPN----LTNLSVPAMFP 131
           N         +NI V N +  G+L  L P    C+         +    L NLS      
Sbjct: 66  N-------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSF----- 125

Query: 132 IADGKNKFRTIGCDALGLV--LGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLE-- 191
                NKF  +GC+A  L+   G+ N   Y++GC+S+C +     N  C+G GCC+ E  
Sbjct: 126 --SPNNKFTLVGCNAWALLSTFGIQN---YSTGCMSLC-DTPPPPNSKCNGVGCCRTEVS 185

Query: 192 IPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFED----EEVEVV 251
           IP     +  Q     N T   +F+ C YAF   D  F FS   +E+ +D        V+
Sbjct: 186 IPLDSHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFS--SLEDLKDLRNVTRFPVL 245

Query: 252 LGWAIGKET------KNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDE 311
           L W+IG +T      +N+CG N+T    F     + Y C+CL GF+GNPYL  GCQD++E
Sbjct: 246 LDWSIGNQTCEQVVGRNICGGNST---CFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINE 305

Query: 312 CKDGRRNDCIYKNKCVNTIGNYTCNCPN 320
           C   R ++C   + C NT+G++ C CP+
Sbjct: 306 CTT-RIHNCSDTSTCENTLGSFHCQCPS 309

BLAST of Lcy09g011030 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 7.2e-38
Identity = 105/320 (32.81%), Postives = 164/320 (51.25%), Query Frame = 0

Query: 26  VVASQALPG--CDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDGNKPPKEFLKNTN 85
           +V  Q  PG  C   CG++ I YPFG  +GCY   NE+FSITC +   ++P       ++
Sbjct: 21  LVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPH----VLSD 80

Query: 86  ISVTNISMDGELHILQPIVRDCH----KPVAGPFVPNLTNLSVPAMFPIADGKNKFRTIG 145
           I V N +  G+L +L      C+    K         L NLS+ A        NK   +G
Sbjct: 81  IEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSA-------NNKLTAVG 140

Query: 146 CDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQVIT-- 205
           C+AL L L      +Y++ C+S+C ++    +G C+G GCC++++   L   T +  +  
Sbjct: 141 CNALSL-LDTFGMQNYSTACLSLC-DSPPEADGECNGRGCCRVDVSAPLDSYTFETTSGR 200

Query: 206 VLNYTRAPNFSSCGYAFVTGDEGFEFSRT-DIENFED-EEVEVVLGWAIGKET------K 265
           + + T   +FS C YAF+  D+ F FS T D+ N  +     V+L W++G +T       
Sbjct: 201 IKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGST 260

Query: 266 NVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDG---RRNDCIYKN 325
           ++CG N+T   S   +G   Y C+C +GF+GNPYL  GCQD++EC       R++C    
Sbjct: 261 SICGGNSTCLDSTPRNG---YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPK 320

BLAST of Lcy09g011030 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.4e-33
Identity = 103/320 (32.19%), Postives = 154/320 (48.12%), Query Frame = 0

Query: 24  LAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNE--TFSITCNKTDGNKPPKEFLKNTN 83
           + +V  Q LP C E CG+V + YPFG   GC+  E  +F+++C          E L    
Sbjct: 17  MQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC--------VNENLFYKG 76

Query: 84  ISVTNISMDGELHILQPIVRDCH----KPVAGPFV-PNLTNLSVPAMFPIADGKNKFRTI 143
           + V  IS   +L +L P    C+    K   G +   NL NL++        G N    +
Sbjct: 77  LEVVEISHSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTL-------SGNNTITAL 136

Query: 144 GCDALGLVLGVHNGSDYAS-GCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQVIT 203
           GC++   V    NG+   S GCIS C   S   NG C+G GCCQ  +P G   L ++   
Sbjct: 137 GCNSYAFVSS--NGTRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYR 196

Query: 204 VLNYTRAPNFS--SCGYAFVTGDEGFEFSRTDIENF---EDEEVEVVLGWAIGKET---- 263
             N T     S   C YAF+  +  F+++ +D  ++    +    VVL W+I  ET    
Sbjct: 197 FDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQV 256

Query: 264 -KNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDG---RRNDCIY 323
            +  CG N   ++S S  G   Y C+C  GF+GNPYL  GCQD++EC       +++C  
Sbjct: 257 GEKKCGVNGICSNSASGIG---YTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSG 316

BLAST of Lcy09g011030 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 3.1e-33
Identity = 104/328 (31.71%), Postives = 156/328 (47.56%), Query Frame = 0

Query: 22  TALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDGNKPPKEFLKN 81
           T L     Q    C   CG+V I YPFG   GCY   ++ F++TC         ++ L  
Sbjct: 18  TQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC------VVEEKLLLF 77

Query: 82  TNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTN-----LSVPAMFPIADGKNKFR 141
             I VTNIS  G + +L     +C++        N TN       + + F ++   NKF 
Sbjct: 78  GIIQVTNISHSGHVSVLFERFSECYEQ------KNETNGTALGYQLGSSFSLS-SNNKFT 137

Query: 142 TIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLE---IPNG------ 201
            +GC+AL L L      +Y++GC+S+C N+    NG C+G GCC  E   +P        
Sbjct: 138 LVGCNALSL-LSTFGKQNYSTGCLSLC-NSQPEANGRCNGVGCCTTEDFSVPFDSDTFQF 197

Query: 202 ----LRDLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEF-SRTDIENFED-EEVEVVLG 261
               LR+     + + N T    F+ C YAF+  D  F F S  D++N  +     V L 
Sbjct: 198 GSVRLRNQVNNSLDLFN-TSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPVALD 257

Query: 262 WAIGKET------KNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECK 321
           W+IG +T        +CG N++  +S + +G   Y C+C +G++GNPY  +GC+D+DEC 
Sbjct: 258 WSIGNQTCEQAGSTRICGKNSSCYNSTTRNG---YICKCNEGYDGNPYRSEGCKDIDECI 317

BLAST of Lcy09g011030 vs. ExPASy TrEMBL
Match: A0A6J1FG68 (wall-associated receptor kinase-like 8 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445414 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.7e-130
Identity = 230/345 (66.67%), Postives = 269/345 (77.97%), Query Frame = 0

Query: 9   LVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTD 68
           L+ L +MKIA ++TA AVVASQALP CDEWCGD+QIPYPFG R GCYLNETF +TCNKT 
Sbjct: 8   LIALILMKIA-ITTAAAVVASQALPDCDEWCGDMQIPYPFGVRKGCYLNETFLVTCNKT- 67

Query: 69  GNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIAD 128
              PPK FLK+TNISVTNIS+ GELH++QPIVRDC+K V GP +PN  NL+VPA FPIA 
Sbjct: 68  -TNPPKAFLKDTNISVTNISISGELHMMQPIVRDCYKHVDGPLLPNEANLTVPAAFPIAY 127

Query: 129 GKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRD 188
            +NKF  IGC+  GL+ GV +GS Y SGC+SMC N+S   +G C GNGCCQLEIP GL D
Sbjct: 128 ERNKFIAIGCNTFGLIGGVVDGSAYVSGCVSMCTNDSKKADGACVGNGCCQLEIPRGLSD 187

Query: 189 LTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKN 248
           L L V ++LN+T A +F+ CGYAFV GDEGF+FS   I +F+D EVEVV GWAIG +T  
Sbjct: 188 LILSVGSLLNHTDAMDFNPCGYAFVVGDEGFQFSTNYITSFDDVEVEVVSGWAIGNDTNF 247

Query: 249 VCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVN 308
           VCG N+  NSSFSDDGSE +RC+C DGFEGNPYLP+GCQD+DECKD R N+C YKNKC+N
Sbjct: 248 VCGLNSVRNSSFSDDGSE-FRCECFDGFEGNPYLPRGCQDIDECKDERLNNCKYKNKCIN 307

Query: 309 TIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIGEF 354
           TIGNYTC+CP NFKGDGR      H GEGCIRD  +   IIIG +
Sbjct: 308 TIGNYTCDCPKNFKGDGR------HGGEGCIRDVKAFTPIIIGGY 342

BLAST of Lcy09g011030 vs. ExPASy TrEMBL
Match: A0A6J1FMZ5 (wall-associated receptor kinase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445414 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 6.4e-130
Identity = 230/343 (67.06%), Postives = 268/343 (78.13%), Query Frame = 0

Query: 9   LVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTD 68
           L+ L +MKIA ++TA AVVASQALP CDEWCGD+QIPYPFG R GCYLNETF +TCNKT 
Sbjct: 8   LIALILMKIA-ITTAAAVVASQALPDCDEWCGDMQIPYPFGVRKGCYLNETFLVTCNKT- 67

Query: 69  GNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIAD 128
              PPK FLK+TNISVTNIS+ GELH++QPIVRDC+K V GP +PN  NL+VPA FPIA 
Sbjct: 68  -TNPPKAFLKDTNISVTNISISGELHMMQPIVRDCYKHVDGPLLPNEANLTVPAAFPIAY 127

Query: 129 GKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRD 188
            +NKF  IGC+  GL+ GV +GS Y SGC+SMC N+S   +G C GNGCCQLEIP GL D
Sbjct: 128 ERNKFIAIGCNTFGLIGGVVDGSAYVSGCVSMCTNDSKKADGACVGNGCCQLEIPRGLSD 187

Query: 189 LTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKN 248
           L L V ++LN+T A +F+ CGYAFV GDEGF+FS   I +F+D EVEVV GWAIG +T  
Sbjct: 188 LILSVGSLLNHTDAMDFNPCGYAFVVGDEGFQFSTNYITSFDDVEVEVVSGWAIGNDTNF 247

Query: 249 VCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVN 308
           VCG N+  NSSFSDDGSE +RC+C DGFEGNPYLP+GCQD+DECKD R N+C YKNKC+N
Sbjct: 248 VCGLNSVRNSSFSDDGSE-FRCECFDGFEGNPYLPRGCQDIDECKDERLNNCKYKNKCIN 307

Query: 309 TIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIG 352
           TIGNYTC+CP NFKGDGR      H GEGCIRD  +   IIIG
Sbjct: 308 TIGNYTCDCPKNFKGDGR------HGGEGCIRDVKAFTPIIIG 340

BLAST of Lcy09g011030 vs. ExPASy TrEMBL
Match: A0A0A0KDF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G351870 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 6.8e-124
Identity = 225/353 (63.74%), Postives = 263/353 (74.50%), Query Frame = 0

Query: 1   MRRSTKTTLVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETF 60
           M+R T T +  + +    +LSTA+ V +SQALP CDEWCGDVQIPYPFG + GCYLN+TF
Sbjct: 1   MKRWTNTVIRVVVI----ILSTAIVVASSQALPDCDEWCGDVQIPYPFGVKQGCYLNQTF 60

Query: 61  SITCNKTDGNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKP--VAGPFVPNLTNL 120
           SITCNKT    PPK FL NTNISVTNIS++GELHILQPIVRDC++   V G  VP +T+L
Sbjct: 61  SITCNKT--VSPPKAFLMNTNISVTNISLNGELHILQPIVRDCYEQGIVIGSSVPTVTDL 120

Query: 121 SVPAMFPIADGKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCC 180
            VPAMFPIADGKNKF  IGCD  GL+ G  NGS Y SGCISMC N S+I N TC GNGCC
Sbjct: 121 LVPAMFPIADGKNKFIAIGCDTFGLIGGELNGSGYVSGCISMCLNESMIGNDTCLGNGCC 180

Query: 181 QLEIPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVL 240
           +LEIPN L +L L V +  N++   +F+ CGYAFV G+EGFEF    I +F+D EVEVV+
Sbjct: 181 ELEIPNELMNLKLLVGSFFNHSSVKDFNPCGYAFVVGNEGFEFDSKYIRSFQDVEVEVVV 240

Query: 241 GWAIGKETKNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRN 300
           GWAIG  +  VCG N+  N SFS+DG E +RCQCL+GF+GNPYLPQGCQD+DECKD   N
Sbjct: 241 GWAIGNGSNYVCGLNSKRNYSFSNDGHE-FRCQCLEGFQGNPYLPQGCQDIDECKDETLN 300

Query: 301 DCIYKNKCVNTIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIG 352
            C Y +KCVNTIGNYTC CP NFKGDGR      +EG GC RDS + I IIIG
Sbjct: 301 QCKYNSKCVNTIGNYTCKCPKNFKGDGR------NEGVGCTRDSKTFIPIIIG 340

BLAST of Lcy09g011030 vs. ExPASy TrEMBL
Match: A0A5D3D516 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold390G00070 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 5.8e-123
Identity = 222/354 (62.71%), Postives = 265/354 (74.86%), Query Frame = 0

Query: 1   MRRSTKTTLVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETF 60
           M+R T T +  + +    +LST + V +SQALPGCDEWCGDVQIPYPFG + GCYLN+TF
Sbjct: 1   MKRWTNTLIRAVVI----ILSTTIVVASSQALPGCDEWCGDVQIPYPFGVKQGCYLNQTF 60

Query: 61  SITCNKTDGNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKP--VAGPFVPNLTNL 120
           SITC+KT    PPK FL NTNISVTNIS++GELHI QPIVRDC++   V G  VP  T+L
Sbjct: 61  SITCSKT--ISPPKAFLMNTNISVTNISLNGELHISQPIVRDCYRQGIVIGSSVPTATDL 120

Query: 121 SVPAMFPIADGKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCC 180
            VPAMFPIADGKNKF  IGCD  GL+ GV NGS Y SGCISMC N S+I N  C GNGCC
Sbjct: 121 LVPAMFPIADGKNKFIAIGCDTFGLIGGVLNGSGYVSGCISMCLNESMIGNEKCLGNGCC 180

Query: 181 QLEIPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVL 240
           +LEIPNGL +L L V +  N++   +F+ CGYAFV G+EGFEF    + +F+D EVEVV+
Sbjct: 181 ELEIPNGLMNLKLLVGSFFNHSLVKDFNPCGYAFVVGNEGFEFDSKYVRSFQDVEVEVVV 240

Query: 241 GWAIGKETKNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRN 300
           GWAIG E+  VCG N+  N +FS+DG E +RCQCL+GF+GNPYLPQGCQD+DECKD   N
Sbjct: 241 GWAIGNESNYVCGLNSKRNYNFSNDGRE-FRCQCLEGFQGNPYLPQGCQDIDECKDETLN 300

Query: 301 DCIYKNKCVNTIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLIL-IIIG 352
            C YK+KCVNTIGNYTC CP +FKGDGR      + GEGC RDS + ++ IIIG
Sbjct: 301 QCKYKSKCVNTIGNYTCKCPKHFKGDGR------NGGEGCTRDSKTFVIPIIIG 341

BLAST of Lcy09g011030 vs. ExPASy TrEMBL
Match: A0A6J1H6B0 (uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC111460594 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 6.6e-119
Identity = 216/339 (63.72%), Postives = 255/339 (75.22%), Query Frame = 0

Query: 14  VMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTDGNKPP 73
           +M+IA+LSTALA+VASQALP CD+ CGDV IPYPFG + GCYLN+ FSITCNKTD N PP
Sbjct: 11  IMEIAILSTALAMVASQALPQCDDRCGDVHIPYPFGIKEGCYLNQNFSITCNKTDRNGPP 70

Query: 74  KEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIADGKNKF 133
           K +L  TNISVTNIS +GELH+LQP+VR C+  V G FVPN +NLSVPAMF I+  KNKF
Sbjct: 71  KAYLMQTNISVTNISTNGELHVLQPVVRTCYDQVNGSFVPNESNLSVPAMFSISGTKNKF 130

Query: 134 RTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQV 193
            TIGC+ L ++ G H GS Y SGCIS+C N S + NG CSGNGCCQLE PNGL +L++ V
Sbjct: 131 VTIGCNTLSVITGNHQGSLYGSGCISVCLNKSSVVNGPCSGNGCCQLEFPNGLTNLSVAV 190

Query: 194 ITVLNYTRAPNFSSCGYAFVTGDE-GFEFSRTDIENFEDEEVEVVLGWAIGKETKNVCGS 253
             +LN+T    F+ CGYAFV  +E  FEF  + IENFEDEEVEVVL W I  ET+  CGS
Sbjct: 191 GGLLNFTDVRTFNPCGYAFVIEEEKRFEFLSSYIENFEDEEVEVVLDWGIRNETRFECGS 250

Query: 254 NNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVNTIGN 313
           N T +S    DG+E YRCQCLDG++GNPYLPQGCQD+DEC+    NDC YK+KC NT GN
Sbjct: 251 NTTRSSIL--DGTE-YRCQCLDGYDGNPYLPQGCQDVDECEYAWLNDCEYKDKCANTKGN 310

Query: 314 YTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIG 352
           YTC+CP NF GDGR        GEGC ++S+S I IIIG
Sbjct: 311 YTCHCPKNFHGDGR------EGGEGCTKNSTSSIPIIIG 340

BLAST of Lcy09g011030 vs. NCBI nr
Match: XP_038886468.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 476.5 bits (1225), Expect = 2.0e-130
Identity = 229/340 (67.35%), Postives = 261/340 (76.76%), Query Frame = 0

Query: 14  VMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTDGNKPP 73
           ++ ++  ST + + +SQAL GCDEWCGD+QIPYPFG + GCYLN+TF ITCNKT+G  PP
Sbjct: 2   MIMLSTTSTVIVIASSQALAGCDEWCGDLQIPYPFGVKEGCYLNKTFLITCNKTNG--PP 61

Query: 74  KEFLKNTNISVTNISMDGELHILQPIVRDCHKPVA--GPFVPNLTNLSVPAMFPIADGKN 133
           K FL NTNISVTNIS+ GELHILQPIVRDC+  V   GPFVPN TNL  PAMFPIADGKN
Sbjct: 62  KAFLMNTNISVTNISLHGELHILQPIVRDCYNQVQENGPFVPNTTNLLAPAMFPIADGKN 121

Query: 134 KFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTL 193
           KF  IGCD  GL+ GV NGS Y SGCISMC N S I N +C GNGCCQ+EIPNGLR+LTL
Sbjct: 122 KFIAIGCDTYGLIGGVLNGSGYVSGCISMCLNESTIGNDSCFGNGCCQMEIPNGLRNLTL 181

Query: 194 QVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKNVCG 253
            V +  N+T   NF+ CGYAFV GDEGFEF    I +FED +VEVV+GWAIG ++  VCG
Sbjct: 182 LVGSFFNHTLVKNFNPCGYAFVVGDEGFEFESRYIRSFEDVKVEVVVGWAIGNDSNYVCG 241

Query: 254 SNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVNTIG 313
            N+  NSSFS D +E +RCQCLDGFEGNPYLPQGCQD+DECKD   N C YKNKCVNTIG
Sbjct: 242 LNSERNSSFSHDRAE-FRCQCLDGFEGNPYLPQGCQDIDECKDETLNHCKYKNKCVNTIG 301

Query: 314 NYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIG 352
           NYTCNCP N+KGD R      + GEGC RDS + I IIIG
Sbjct: 302 NYTCNCPTNYKGDAR------YGGEGCTRDSKAFIPIIIG 332

BLAST of Lcy09g011030 vs. NCBI nr
Match: XP_022939536.1 (wall-associated receptor kinase-like 8 isoform X2 [Cucurbita moschata])

HSP 1 Score: 474.6 bits (1220), Expect = 7.7e-130
Identity = 230/345 (66.67%), Postives = 269/345 (77.97%), Query Frame = 0

Query: 9   LVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTD 68
           L+ L +MKIA ++TA AVVASQALP CDEWCGD+QIPYPFG R GCYLNETF +TCNKT 
Sbjct: 8   LIALILMKIA-ITTAAAVVASQALPDCDEWCGDMQIPYPFGVRKGCYLNETFLVTCNKT- 67

Query: 69  GNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIAD 128
              PPK FLK+TNISVTNIS+ GELH++QPIVRDC+K V GP +PN  NL+VPA FPIA 
Sbjct: 68  -TNPPKAFLKDTNISVTNISISGELHMMQPIVRDCYKHVDGPLLPNEANLTVPAAFPIAY 127

Query: 129 GKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRD 188
            +NKF  IGC+  GL+ GV +GS Y SGC+SMC N+S   +G C GNGCCQLEIP GL D
Sbjct: 128 ERNKFIAIGCNTFGLIGGVVDGSAYVSGCVSMCTNDSKKADGACVGNGCCQLEIPRGLSD 187

Query: 189 LTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKN 248
           L L V ++LN+T A +F+ CGYAFV GDEGF+FS   I +F+D EVEVV GWAIG +T  
Sbjct: 188 LILSVGSLLNHTDAMDFNPCGYAFVVGDEGFQFSTNYITSFDDVEVEVVSGWAIGNDTNF 247

Query: 249 VCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVN 308
           VCG N+  NSSFSDDGSE +RC+C DGFEGNPYLP+GCQD+DECKD R N+C YKNKC+N
Sbjct: 248 VCGLNSVRNSSFSDDGSE-FRCECFDGFEGNPYLPRGCQDIDECKDERLNNCKYKNKCIN 307

Query: 309 TIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIGEF 354
           TIGNYTC+CP NFKGDGR      H GEGCIRD  +   IIIG +
Sbjct: 308 TIGNYTCDCPKNFKGDGR------HGGEGCIRDVKAFTPIIIGGY 342

BLAST of Lcy09g011030 vs. NCBI nr
Match: XP_022939535.1 (wall-associated receptor kinase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 473.8 bits (1218), Expect = 1.3e-129
Identity = 230/343 (67.06%), Postives = 268/343 (78.13%), Query Frame = 0

Query: 9   LVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTD 68
           L+ L +MKIA ++TA AVVASQALP CDEWCGD+QIPYPFG R GCYLNETF +TCNKT 
Sbjct: 8   LIALILMKIA-ITTAAAVVASQALPDCDEWCGDMQIPYPFGVRKGCYLNETFLVTCNKT- 67

Query: 69  GNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIAD 128
              PPK FLK+TNISVTNIS+ GELH++QPIVRDC+K V GP +PN  NL+VPA FPIA 
Sbjct: 68  -TNPPKAFLKDTNISVTNISISGELHMMQPIVRDCYKHVDGPLLPNEANLTVPAAFPIAY 127

Query: 129 GKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRD 188
            +NKF  IGC+  GL+ GV +GS Y SGC+SMC N+S   +G C GNGCCQLEIP GL D
Sbjct: 128 ERNKFIAIGCNTFGLIGGVVDGSAYVSGCVSMCTNDSKKADGACVGNGCCQLEIPRGLSD 187

Query: 189 LTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKN 248
           L L V ++LN+T A +F+ CGYAFV GDEGF+FS   I +F+D EVEVV GWAIG +T  
Sbjct: 188 LILSVGSLLNHTDAMDFNPCGYAFVVGDEGFQFSTNYITSFDDVEVEVVSGWAIGNDTNF 247

Query: 249 VCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVN 308
           VCG N+  NSSFSDDGSE +RC+C DGFEGNPYLP+GCQD+DECKD R N+C YKNKC+N
Sbjct: 248 VCGLNSVRNSSFSDDGSE-FRCECFDGFEGNPYLPRGCQDIDECKDERLNNCKYKNKCIN 307

Query: 309 TIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIG 352
           TIGNYTC+CP NFKGDGR      H GEGCIRD  +   IIIG
Sbjct: 308 TIGNYTCDCPKNFKGDGR------HGGEGCIRDVKAFTPIIIG 340

BLAST of Lcy09g011030 vs. NCBI nr
Match: KAG6578932.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 470.7 bits (1210), Expect = 1.1e-128
Identity = 229/342 (66.96%), Postives = 267/342 (78.07%), Query Frame = 0

Query: 9   LVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKTD 68
           L+ L +MKIA ++TA AVVASQALP CDEWCGD+QIPYPFG R GCYLNETF +TCNKT 
Sbjct: 8   LIALILMKIA-ITTAAAVVASQALPDCDEWCGDMQIPYPFGVRKGCYLNETFLVTCNKT- 67

Query: 69  GNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIAD 128
              PPK FLK+TNISVTNIS+ GELH++QPIVRDC+K V GP +PN  NL+VPA FPIA 
Sbjct: 68  -TNPPKAFLKDTNISVTNISISGELHMMQPIVRDCYKHVDGPLLPNEANLTVPAAFPIAY 127

Query: 129 GKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRD 188
            +NKF  IGC+  GL+ GV +GS Y SGC+SMC N+S   +G C GNGCCQLEIP GL D
Sbjct: 128 ERNKFIAIGCNTFGLIGGVVDGSAYVSGCVSMCTNDSKKADGACVGNGCCQLEIPRGLSD 187

Query: 189 LTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETKN 248
           L L V ++LN+T A +F+ CGYAFV GDEGF+FS   I +F+D EVEVV GWAIG +T  
Sbjct: 188 LILSVGSLLNHTDAMDFNPCGYAFVVGDEGFQFSSNYITSFDDVEVEVVGGWAIGNDTNF 247

Query: 249 VCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVN 308
           VCG N+  NSSFSDDGSE +RC+C DGFEGNPYLP+GCQD+DECKD R N+C YKNKC+N
Sbjct: 248 VCGLNSVRNSSFSDDGSE-FRCECFDGFEGNPYLPRGCQDIDECKDERLNNCKYKNKCIN 307

Query: 309 TIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIII 351
           TIGNYTC+CP NFKGDGR      H GEGCIRD  +   III
Sbjct: 308 TIGNYTCDCPKNFKGDGR------HGGEGCIRDVKAFTPIII 339

BLAST of Lcy09g011030 vs. NCBI nr
Match: XP_023550660.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.5 bits (1186), Expect = 6.7e-126
Identity = 223/344 (64.83%), Postives = 265/344 (77.03%), Query Frame = 0

Query: 9   LVPLTVMKIAMLST-ALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCNKT 68
           L+ L ++ IA+LST A A++ASQALP C EWCGD+QIPYPFG R GCYLNETF ITC KT
Sbjct: 8   LMALILINIAILSTAAAAIIASQALPDCHEWCGDMQIPYPFGVRKGCYLNETFLITCTKT 67

Query: 69  DGNKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIA 128
               PPK FLK+TNISVTNIS+ GELH++QPIVRDC+K V GP +P+  NL++PA FPIA
Sbjct: 68  --TNPPKAFLKDTNISVTNISISGELHMMQPIVRDCYKHVDGPLLPDQANLTLPATFPIA 127

Query: 129 DGKNKFRTIGCDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLR 188
             +NKF  +GC+  GL+ GV +GS Y SGC+SMC N+S   +G C GNGCCQLEIP GL 
Sbjct: 128 YERNKFIAVGCNTFGLIGGVVDGSAYVSGCVSMCTNDSKKADGACVGNGCCQLEIPRGLS 187

Query: 189 DLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFEDEEVEVVLGWAIGKETK 248
           DL L V ++LN+T A +F+ CGYAFV  DEGF+FS   I +F+D EVEVV GWAIG +T 
Sbjct: 188 DLILSVGSLLNHTDAMDFNPCGYAFVVADEGFQFSSNYITSFDDVEVEVVAGWAIGNDTN 247

Query: 249 NVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCV 308
            VCG N+  NSSFSDDGSE +RC+C DGFEGNPYLP+GCQD+DECKD R N+C YKNKC+
Sbjct: 248 FVCGPNSVRNSSFSDDGSE-FRCECFDGFEGNPYLPRGCQDIDECKDERLNNCKYKNKCI 307

Query: 309 NTIGNYTCNCPNNFKGDGRHWRHGGHEGEGCIRDSSSLILIIIG 352
           NTIGNYTC+CP NFKGDGR      H GEGCIRD  +   IIIG
Sbjct: 308 NTIGNYTCDCPKNFKGDGR------HGGEGCIRDVKAFTPIIIG 342

BLAST of Lcy09g011030 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 189.9 bits (481), Expect = 3.5e-48
Identity = 109/309 (35.28%), Postives = 167/309 (54.05%), Query Frame = 0

Query: 26  VVASQALPGCDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDGNKPPKEFLKNTNIS 85
           +V  Q    C   CG+V + YPFGT  GCY   +E+F++TCN+       +E L   N+ 
Sbjct: 20  LVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNE-------QEKLFFGNMP 79

Query: 86  VTNISMDGELHILQPIVRDCHKPVAGPFVPNLTNLSVPAMFPIADGKNKFRTIGCDALGL 145
           V N+S+ G+L +     R C+    G     +   +    F +++  N+F  +GC++   
Sbjct: 80  VINMSLSGQLRVRLVRSRVCYDS-QGKQTDYIAQRTTLGNFTLSE-LNRFTVVGCNSYAF 139

Query: 146 VLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQVITVLNYTRAP 205
            L       Y++GCIS+C +++   NG+CSG GCCQ+ +P G   + ++  +  N+    
Sbjct: 140 -LRTSGVEKYSTGCISIC-DSATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVH 199

Query: 206 NFSSCGYAFVTGDEGFEF-SRTDIENFED-EEVEVVLGWAIGKET------KNVCGSNNT 265
            F+ C YAF+  D  F+F +  D+ N  +     VVL W+IG +T      + VCG N+T
Sbjct: 200 LFNPCTYAFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNST 259

Query: 266 WNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDGRRNDCIYKNKCVNTIGNYTC 325
               F   G   Y C+CL+GFEGNPYLP GCQD++EC   R N C   + C NT G++ C
Sbjct: 260 ---CFDSTGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHN-CSEHSTCENTKGSFNC 313

BLAST of Lcy09g011030 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 160.6 bits (405), Expect = 2.3e-39
Identity = 111/328 (33.84%), Postives = 159/328 (48.48%), Query Frame = 0

Query: 12  LTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDG 71
           L +M I        +V +Q    C   CGDV I YPFG   GCY   +++F+ITC +   
Sbjct: 6   LFLMAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEEDKP 65

Query: 72  NKPPKEFLKNTNISVTNISMDGELHILQPIVRDCHKPVAGPFVPN----LTNLSVPAMFP 131
           N         +NI V N +  G+L  L P    C+         +    L NLS      
Sbjct: 66  N-------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSF----- 125

Query: 132 IADGKNKFRTIGCDALGLV--LGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLE-- 191
                NKF  +GC+A  L+   G+ N   Y++GC+S+C +     N  C+G GCC+ E  
Sbjct: 126 --SPNNKFTLVGCNAWALLSTFGIQN---YSTGCMSLC-DTPPPPNSKCNGVGCCRTEVS 185

Query: 192 IPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGDEGFEFSRTDIENFED----EEVEVV 251
           IP     +  Q     N T   +F+ C YAF   D  F FS   +E+ +D        V+
Sbjct: 186 IPLDSHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFS--SLEDLKDLRNVTRFPVL 245

Query: 252 LGWAIGKET------KNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDE 311
           L W+IG +T      +N+CG N+T    F     + Y C+CL GF+GNPYL  GCQD++E
Sbjct: 246 LDWSIGNQTCEQVVGRNICGGNST---CFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINE 305

Query: 312 CKDGRRNDCIYKNKCVNTIGNYTCNCPN 320
           C   R ++C   + C NT+G++ C CP+
Sbjct: 306 CTT-RIHNCSDTSTCENTLGSFHCQCPS 309

BLAST of Lcy09g011030 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 159.5 bits (402), Expect = 5.1e-39
Identity = 105/320 (32.81%), Postives = 164/320 (51.25%), Query Frame = 0

Query: 26  VVASQALPG--CDEWCGDVQIPYPFGTRNGCYL--NETFSITCNKTDGNKPPKEFLKNTN 85
           +V  Q  PG  C   CG++ I YPFG  +GCY   NE+FSITC +   ++P       ++
Sbjct: 21  LVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPH----VLSD 80

Query: 86  ISVTNISMDGELHILQPIVRDCH----KPVAGPFVPNLTNLSVPAMFPIADGKNKFRTIG 145
           I V N +  G+L +L      C+    K         L NLS+ A        NK   +G
Sbjct: 81  IEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSA-------NNKLTAVG 140

Query: 146 CDALGLVLGVHNGSDYASGCISMCFNNSIINNGTCSGNGCCQLEIPNGLRDLTLQVIT-- 205
           C+AL L L      +Y++ C+S+C ++    +G C+G GCC++++   L   T +  +  
Sbjct: 141 CNALSL-LDTFGMQNYSTACLSLC-DSPPEADGECNGRGCCRVDVSAPLDSYTFETTSGR 200

Query: 206 VLNYTRAPNFSSCGYAFVTGDEGFEFSRT-DIENFED-EEVEVVLGWAIGKET------K 265
           + + T   +FS C YAF+  D+ F FS T D+ N  +     V+L W++G +T       
Sbjct: 201 IKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGST 260

Query: 266 NVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQDMDECKDG---RRNDCIYKN 325
           ++CG N+T   S   +G   Y C+C +GF+GNPYL  GCQD++EC       R++C    
Sbjct: 261 SICGGNSTCLDSTPRNG---YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPK 320

BLAST of Lcy09g011030 vs. TAIR 10
Match: AT1G16260.1 (Wall-associated kinase family protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-30
Identity = 104/327 (31.80%), Postives = 148/327 (45.26%), Query Frame = 0

Query: 6   KTTLVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCN 65
           K  LV + +++I     A A     AL  C + CG+V +PYPFG   GCY N+ F I C 
Sbjct: 6   KRFLVVMLLLRIC--EYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVC- 65

Query: 66  KTDGNKPPKEFLKNTNISVTNISMDGELHI-------LQPIVRDCHKPVAGPFVPNLTNL 125
           K+  ++ P   L     +VT+ ++     I       +Q  ++    P    +  +  NL
Sbjct: 66  KSSSDQQPILLLPRIRRAVTSFNLGDPFSISVYNKFYIQSPLKHSGCPNRDGYSSSSLNL 125

Query: 126 SVPAMFPIADGKNKFRTIGCD--ALGLVLGVHNGSDYASGCISMCFNNSIINNG---TCS 185
                F      NKF  +GC+  A   V G+        GC + C N      G   +C 
Sbjct: 126 KGSPFF--ISENNKFTAVGCNNKAFMNVTGLQ-----IVGCETTCGNEIRSYKGANTSCV 185

Query: 186 GNGCCQLEIPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGD--EGFEFSRTDIENFED 245
           G  CCQ+ IP  L+   LQV         PN   C  AF+T     G  F+  ++  +  
Sbjct: 186 GYKCCQMTIPPLLQ---LQVFDATVEKLEPNKQGCQVAFLTQFTLSGSLFTPPELMEY-S 245

Query: 246 EEVEVVLGWAIG---KETKNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQD 305
           E   + L W +      +K V    NT+   F D     Y+C C +G+EGNPY+P GCQD
Sbjct: 246 EYTTIELEWRLDLSYMTSKRVLCKGNTF---FEDS----YQCSCHNGYEGNPYIPGGCQD 305

Query: 306 MDECKDGRRNDCIYKNKCVNTIGNYTC 316
           +DEC+D   N C  K KCVN +G+Y C
Sbjct: 306 IDECRDPHLNKC-GKRKCVNVLGSYRC 310

BLAST of Lcy09g011030 vs. TAIR 10
Match: AT1G16260.2 (Wall-associated kinase family protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-30
Identity = 104/327 (31.80%), Postives = 148/327 (45.26%), Query Frame = 0

Query: 6   KTTLVPLTVMKIAMLSTALAVVASQALPGCDEWCGDVQIPYPFGTRNGCYLNETFSITCN 65
           K  LV + +++I     A A     AL  C + CG+V +PYPFG   GCY N+ F I C 
Sbjct: 6   KRFLVVMLLLRIC--EYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVC- 65

Query: 66  KTDGNKPPKEFLKNTNISVTNISMDGELHI-------LQPIVRDCHKPVAGPFVPNLTNL 125
           K+  ++ P   L     +VT+ ++     I       +Q  ++    P    +  +  NL
Sbjct: 66  KSSSDQQPILLLPRIRRAVTSFNLGDPFSISVYNKFYIQSPLKHSGCPNRDGYSSSSLNL 125

Query: 126 SVPAMFPIADGKNKFRTIGCD--ALGLVLGVHNGSDYASGCISMCFNNSIINNG---TCS 185
                F      NKF  +GC+  A   V G+        GC + C N      G   +C 
Sbjct: 126 KGSPFF--ISENNKFTAVGCNNKAFMNVTGLQ-----IVGCETTCGNEIRSYKGANTSCV 185

Query: 186 GNGCCQLEIPNGLRDLTLQVITVLNYTRAPNFSSCGYAFVTGD--EGFEFSRTDIENFED 245
           G  CCQ+ IP  L+   LQV         PN   C  AF+T     G  F+  ++  +  
Sbjct: 186 GYKCCQMTIPPLLQ---LQVFDATVEKLEPNKQGCQVAFLTQFTLSGSLFTPPELMEY-S 245

Query: 246 EEVEVVLGWAIG---KETKNVCGSNNTWNSSFSDDGSEYYRCQCLDGFEGNPYLPQGCQD 305
           E   + L W +      +K V    NT+   F D     Y+C C +G+EGNPY+P GCQD
Sbjct: 246 EYTTIELEWRLDLSYMTSKRVLCKGNTF---FEDS----YQCSCHNGYEGNPYIPGGCQD 305

Query: 306 MDECKDGRRNDCIYKNKCVNTIGNYTC 316
           +DEC+D   N C  K KCVN +G+Y C
Sbjct: 306 IDECRDPHLNKC-GKRKCVNVLGSYRC 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMP15.0e-4735.28Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q9LMN73.2e-3833.84Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q391917.2e-3832.81Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN62.4e-3332.19Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Q9LMN83.1e-3331.71Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FG683.7e-13066.67wall-associated receptor kinase-like 8 isoform X2 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1FMZ56.4e-13067.06wall-associated receptor kinase 2-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0KDF06.8e-12463.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G351870 PE=4 SV=1[more]
A0A5D3D5165.8e-12362.71Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1H6B06.6e-11963.72uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC1114605... [more]
Match NameE-valueIdentityDescription
XP_038886468.12.0e-13067.35wall-associated receptor kinase 2-like [Benincasa hispida][more]
XP_022939536.17.7e-13066.67wall-associated receptor kinase-like 8 isoform X2 [Cucurbita moschata][more]
XP_022939535.11.3e-12967.06wall-associated receptor kinase 2-like isoform X1 [Cucurbita moschata][more]
KAG6578932.11.1e-12866.96Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023550660.16.7e-12664.83wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G21270.13.5e-4835.28wall-associated kinase 2 [more]
AT1G21230.12.3e-3933.84wall associated kinase 5 [more]
AT1G21250.15.1e-3932.81cell wall-associated kinase [more]
AT1G16260.12.5e-3031.80Wall-associated kinase family protein [more]
AT1G16260.22.5e-3031.80Wall-associated kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 288..339
e-value: 8.9E-4
score: 26.4
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 288..323
e-value: 3.3E-7
score: 30.4
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 35..91
e-value: 1.7E-9
score: 38.0
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 244..328
e-value: 1.3E-11
score: 46.7
NoneNo IPR availablePANTHERPTHR33491OSJNBA0016N04.9 PROTEINcoord: 12..295
NoneNo IPR availablePANTHERPTHR33491:SF5OSJNBA0016N04.9 PROTEINcoord: 12..295
NoneNo IPR availableCDDcd00054EGF_CAcoord: 288..324
e-value: 4.0608E-4
score: 35.6902
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 287..327
IPR013695Wall-associated receptor kinasePFAMPF08488WAKcoord: 170..272
e-value: 5.5E-7
score: 30.1
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 306..317
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 288..315
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 288..327
score: 11.170095

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g011030.1Lcy09g011030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0016310 phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity