Lcy09g009700 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g009700
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr09: 9980871 .. 9981511 (-)
RNA-Seq Expression	Lcy09g009700
Synteny	Lcy09g009700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCCATAATAAACCTATTCCTCCGATTGATGTTCGATGTGCCTGGATTTCTGATTATTTGATGGAATACATATCGACTAATTCGTGCTCCGTTAAGCAGGGCCAACAAGTGGATGAGGTTAGATCACTCGTCCAAGGGAGGGATGAAATGATCATTCATGTGGATGCGGGATGTGATACGAATGCGCGACGTGCTAGTGTCGGTGTGGTTATCCGGGATTCGAGAGAGGTGGCTTGATTGCAGCTTTACATAGTCTTTTAGCATCCTATGTTAATCCCCTATGTGCGGAGGCATCGGCGATTTTAGAAGAACTTCGTCTGGCTGAGCGCATGAATCTCAGCAGAATTAAGATCTTCTCGGATTCGCTTTCCTTGGTGGGGATGTTGAAGAAGGAAATATTAGTCTGCATCGATGTATCCTCGATCGTTTGGGATATTGAAGATATGCGGAAGCGTTTTCAGCTTGTAGAAATCGATTATATAAGTCGAAATTATAATGTCTTGGCTCATTCACTAGCTCGTCAGAGTTTTTGTGATGGTTCGAAGTTGTGGCTATCGAATTTCCCTACTTGGTTATCCAAGATAGCTTGTACTGATTTACATTTGTTTGTATCCCCTCGGGGACTTGGTTGTTTCTAA

mRNA sequence

ATGATCCATAATAAACCTATTCCTCCGATTGATGTTCGATGTGCCTGGATTTCTGATTATTTGATGGAATACATATCGACTAATTCGTGCTCCGTTAAGCAGGGCCAACAAGTGGATGAGGTTAGATCACTCGTCCAAGGGAGGGATGAAATGATCATTCATGTGGATGCGGGATCTTTACATAGTCTTTTAGCATCCTATGTTAATCCCCTATGTGCGGAGGCATCGGCGATTTTAGAAGAACTTCGTCTGGCTGAGCGCATGAATCTCAGCAGAATTAAGATCTTCTCGGATTCGCTTTCCTTGGTGGGGATGTTGAAGAAGGAAATATTAGTCTGCATCGATGTATCCTCGATCGTTTGGGATATTGAAGATATGCGGAAGCGTTTTCAGCTTGTAGAAATCGATTATATAAGTCGAAATTATAATGTCTTGGCTCATTCACTAGCTCGTCAGAGTTTTTGTGATGGTTCGAAGTTGTGGCTATCGAATTTCCCTACTTGGTTATCCAAGATAGCTTGTACTGATTTACATTTGTTTGTATCCCCTCGGGGACTTGGTTGTTTCTAA

Coding sequence (CDS)

ATGATCCATAATAAACCTATTCCTCCGATTGATGTTCGATGTGCCTGGATTTCTGATTATTTGATGGAATACATATCGACTAATTCGTGCTCCGTTAAGCAGGGCCAACAAGTGGATGAGGTTAGATCACTCGTCCAAGGGAGGGATGAAATGATCATTCATGTGGATGCGGGATCTTTACATAGTCTTTTAGCATCCTATGTTAATCCCCTATGTGCGGAGGCATCGGCGATTTTAGAAGAACTTCGTCTGGCTGAGCGCATGAATCTCAGCAGAATTAAGATCTTCTCGGATTCGCTTTCCTTGGTGGGGATGTTGAAGAAGGAAATATTAGTCTGCATCGATGTATCCTCGATCGTTTGGGATATTGAAGATATGCGGAAGCGTTTTCAGCTTGTAGAAATCGATTATATAAGTCGAAATTATAATGTCTTGGCTCATTCACTAGCTCGTCAGAGTTTTTGTGATGGTTCGAAGTTGTGGCTATCGAATTTCCCTACTTGGTTATCCAAGATAGCTTGTACTGATTTACATTTGTTTGTATCCCCTCGGGGACTTGGTTGTTTCTAA

Protein sequence

MIHNKPIPPIDVRCAWISDYLMEYISTNSCSVKQGQQVDEVRSLVQGRDEMIIHVDAGSLHSLLASYVNPLCAEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQLVEIDYISRNYNVLAHSLARQSFCDGSKLWLSNFPTWLSKIACTDLHLFVSPRGLGCF

Homology

[Close]

BLAST of Lcy09g009700 vs. ExPASy TrEMBL
Match: A0A5C7IFK3 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_009054 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-07
Identity = 39/109 (35.78%), Postives = 63/109 (57.80%), Query Frame = 0


Query: 73  AEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQL 132
           AEA AI E + LA    L  + I SDSL++V +   EI    DV +++ DI+ +  R + 
Sbjct: 187 AEAKAIYEGILLATTSGLQSLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 246

Query: 133 VEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFPTWLSKIACTDLHLF 181
           + I YI R+ N++AH +AR++   + S +    +P WL K+A +D+  F
Sbjct: 247 ISISYIPRSCNMVAHEVARRAIGLENSVIMYDPYPIWLQKVALSDVLSF 295

[Close]

BLAST of Lcy09g009700 vs. ExPASy TrEMBL
Match: A0A5C7IST2 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-06
Identity = 40/111 (36.04%), Postives = 61/111 (54.95%), Query Frame = 0


Query: 73  AEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQL 132
           AEA  I E L LA    L  + I SDSL++V +   EI    DV +++ DI+ +  R + 
Sbjct: 290 AEAKVIYEGLLLATSSGLQPLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 349

Query: 133 VEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFPTWLSKIACTDLHLFVS 183
             I YI R+ N +AH +AR++   + S +    +P WL K+A +D+ L  S
Sbjct: 350 TSISYIPRSCNRVAHEVARRAIGLENSVIMYDPYPIWLQKLALSDVLLSFS 400

[Close]

BLAST of Lcy09g009700 vs. ExPASy TrEMBL
Match: M1CY15 (RNase H domain-containing protein OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.3e-06
Identity = 36/98 (36.73%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 71  LCAEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRF 130
           + AEA AI E L  A +   S++ I SD+ +++ ML+K ++   D+ +I  D+  + K +
Sbjct: 44  ITAEALAIREALERALQKGWSKVHILSDAKNVMQMLQKNLITSWDIETICKDVWRLMKSY 103

Query: 131 QLVEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFPT 168
           + ++I YI R +NVLAH+LA+ S  C     W S FP+
Sbjct: 104 EEIKIIYIPRTWNVLAHNLAKISISCINRISWDSAFPS 141

[Close]

BLAST of Lcy09g009700 vs. ExPASy TrEMBL
Match: A0A5C7IVJ7 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001899 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.2e-05
Identity = 42/146 (28.77%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 34  QGQQVDEVRSLVQGRD--------EMIIHVDAGSLHSL----LASYVNPLCAEASAILEE 93
           +G  +D +R++++  D         +II   +GS+ +     L   + P  AEA  IL  
Sbjct: 121 RGYSLDFLRNVLKALDVRSNMVGLGLIIRDPSGSVMASGAHGLELTLKPDVAEAIGILHG 180

Query: 94  LRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQLVEIDYISRN 153
           + LA    L  + + SD+  +V ++  +I V  D+  I++DI D  +R  +  I ++   
Sbjct: 181 INLAMETCLLPLVVESDAQHVVNLINSDISVSSDIGLIIYDIRDCMRRVGIESISFVPMT 240

Query: 154 YNVLAHSLARQSFCD-GSKLWLSNFP 167
            NV+AH+LA+ +  + GS+LWL ++P
Sbjct: 241 TNVVAHNLAKYALLNIGSQLWLESYP 266

[Close]

BLAST of Lcy09g009700 vs. ExPASy TrEMBL
Match: A0A4Y1R838 (Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-05
Identity = 40/132 (30.30%), Postives = 64/132 (48.48%), Query Frame = 0


Query: 48  RDEMIIHVDAGSLHSLLASYVNPLCAEASAILEELRLAERMNLSRIKIFSDSLSLVGMLK 107
           RD +   + AG    +  +  + L AEA A+ E L     +   ++ + SDSLSL+ ML 
Sbjct: 472 RDGIGCFISAGGCGDMRCT--SALMAEAEAVREALTWCLELGFDKVAVESDSLSLIKMLN 531

Query: 108 KEILVCIDVSSIVWDIEDMRKRFQLVEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFP 167
           KE +V +++  I++D+  +  R   VE  Y  R  N  AH++A  +F   G   W    P
Sbjct: 532 KEDVVDLEIEGILFDVHCLSHRAHHVEFLYAPRKCNQAAHNVAAYAFRIGGRHSWDIVCP 591

Query: 168 TWLSKIACTDLH 179
            WL      D++
Sbjct: 592 EWLFNCLAHDIN 601

[Close]

BLAST of Lcy09g009700 vs. NCBI nr
Match: TXG67779.1 (hypothetical protein EZV62_009054 [Acer yangbiense])

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-07
Identity = 39/109 (35.78%), Postives = 63/109 (57.80%), Query Frame = 0


Query: 73  AEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQL 132
           AEA AI E + LA    L  + I SDSL++V +   EI    DV +++ DI+ +  R + 
Sbjct: 187 AEAKAIYEGILLATTSGLQSLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 246

Query: 133 VEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFPTWLSKIACTDLHLF 181
           + I YI R+ N++AH +AR++   + S +    +P WL K+A +D+  F
Sbjct: 247 ISISYIPRSCNMVAHEVARRAIGLENSVIMYDPYPIWLQKVALSDVLSF 295

[Close]

BLAST of Lcy09g009700 vs. NCBI nr
Match: TXG71894.1 (hypothetical protein EZV62_000473 [Acer yangbiense])

HSP 1 Score: 63.2 bits (152), Expect = 2.9e-06
Identity = 40/111 (36.04%), Postives = 61/111 (54.95%), Query Frame = 0


Query: 73  AEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQL 132
           AEA  I E L LA    L  + I SDSL++V +   EI    DV +++ DI+ +  R + 
Sbjct: 290 AEAKVIYEGLLLATSSGLQPLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 349

Query: 133 VEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFPTWLSKIACTDLHLFVS 183
             I YI R+ N +AH +AR++   + S +    +P WL K+A +D+ L  S
Sbjct: 350 TSISYIPRSCNRVAHEVARRAIGLENSVIMYDPYPIWLQKLALSDVLLSFS 400

[Close]

BLAST of Lcy09g009700 vs. NCBI nr
Match: TXG73320.1 (hypothetical protein EZV62_001899 [Acer yangbiense])

HSP 1 Score: 60.1 bits (144), Expect = 2.4e-05
Identity = 42/146 (28.77%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 34  QGQQVDEVRSLVQGRD--------EMIIHVDAGSLHSL----LASYVNPLCAEASAILEE 93
           +G  +D +R++++  D         +II   +GS+ +     L   + P  AEA  IL  
Sbjct: 121 RGYSLDFLRNVLKALDVRSNMVGLGLIIRDPSGSVMASGAHGLELTLKPDVAEAIGILHG 180

Query: 94  LRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRFQLVEIDYISRN 153
           + LA    L  + + SD+  +V ++  +I V  D+  I++DI D  +R  +  I ++   
Sbjct: 181 INLAMETCLLPLVVESDAQHVVNLINSDISVSSDIGLIIYDIRDCMRRVGIESISFVPMT 240

Query: 154 YNVLAHSLARQSFCD-GSKLWLSNFP 167
            NV+AH+LA+ +  + GS+LWL ++P
Sbjct: 241 TNVVAHNLAKYALLNIGSQLWLESYP 266

[Close]

BLAST of Lcy09g009700 vs. NCBI nr
Match: XP_021800524.1 (uncharacterized protein LOC110744812 [Prunus avium])

HSP 1 Score: 59.3 bits (142), Expect = 4.1e-05
Identity = 34/108 (31.48%), Postives = 58/108 (53.70%), Query Frame = 0


Query: 71  LCAEASAILEELRLAERMNLSRIKIFSDSLSLVGMLKKEILVCIDVSSIVWDIEDMRKRF 130
           L AEA AI + L +        +++ SDS  ++ M++ + L   ++  I++DI+ + +RF
Sbjct: 209 LVAEAEAIRDMLGICLHNGFLSVEVESDSQEIIRMIRGDSLSATEIEGILFDIQVLVRRF 268

Query: 131 QLVEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFPTWLSKIACTDL 178
           Q VE  +I R  N  AH +A   F   GS++W  + P WL  +   D+
Sbjct: 269 QWVEFRHIPRLCNNAAHEVAAYFFRAGGSEVWDQSGPAWLHSVLALDV 316

[Close]

BLAST of Lcy09g009700 vs. NCBI nr
Match: BBH00303.1 (Ribonuclease H-like superfamily protein [Prunus dulcis])

HSP 1 Score: 59.3 bits (142), Expect = 4.1e-05
Identity = 40/132 (30.30%), Postives = 64/132 (48.48%), Query Frame = 0


Query: 48  RDEMIIHVDAGSLHSLLASYVNPLCAEASAILEELRLAERMNLSRIKIFSDSLSLVGMLK 107
           RD +   + AG    +  +  + L AEA A+ E L     +   ++ + SDSLSL+ ML 
Sbjct: 472 RDGIGCFISAGGCGDMRCT--SALMAEAEAVREALTWCLELGFDKVAVESDSLSLIKMLN 531

Query: 108 KEILVCIDVSSIVWDIEDMRKRFQLVEIDYISRNYNVLAHSLARQSF-CDGSKLWLSNFP 167
           KE +V +++  I++D+  +  R   VE  Y  R  N  AH++A  +F   G   W    P
Sbjct: 532 KEDVVDLEIEGILFDVHCLSHRAHHVEFLYAPRKCNQAAHNVAAYAFRIGGRHSWDIVCP 591

Query: 168 TWLSKIACTDLH 179
            WL      D++
Sbjct: 592 EWLFNCLAHDIN 601

The following BLAST results are available for this feature:

BLAST of Lcy09g009700 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy09g009700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5C7IFK3	1.2e-07	35.78	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_009054 ...	[more]
A0A5C7IST2	1.4e-06	36.04	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 ...	[more]
M1CY15	5.3e-06	36.73	RNase H domain-containing protein OS=Solanum tuberosum OX=4113 PE=4 SV=1	[more]
A0A5C7IVJ7	1.2e-05	28.77	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001899 ...	[more]
A0A4Y1R838	2.0e-05	30.30	Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255...	[more]

BLAST of Lcy09g009700 vs. NCBI nr


Match Name	E-value	Identity	Description
TXG67779.1	2.6e-07	35.78	hypothetical protein EZV62_009054 [Acer yangbiense]	[more]
TXG71894.1	2.9e-06	36.04	hypothetical protein EZV62_000473 [Acer yangbiense]	[more]
TXG73320.1	2.4e-05	28.77	hypothetical protein EZV62_001899 [Acer yangbiense]	[more]
XP_021800524.1	4.1e-05	31.48	uncharacterized protein LOC110744812 [Prunus avium]	[more]
BBH00303.1	4.1e-05	30.30	Ribonuclease H-like superfamily protein [Prunus dulcis]	[more]

BLAST of Lcy09g009700 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 66..152 e-value: 2.0E-11 score: 43.8
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 30..157 e-value: 1.1E-10 score: 43.6
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 59..177
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 59..177
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 62..151 e-value: 4.13528E-16 score: 68.88
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 51..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g009700.1	Lcy09g009700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity