Lcy09g009560 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g009560
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr09: 9745949 .. 9746530 (+)
RNA-Seq Expression	Lcy09g009560
Synteny	Lcy09g009560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAGCTTTTTACCTCAAGACTCAAGCCTCAAAAAGGCCAGGGAACCACACGAGTCAGCCTCGTGGAACGAAACCTTGGGGCGCGGAGGTCTAGGTTGGATCGTGCGTGACTCGGATGGATCTTTGGTCTATTTCGGAATGCAAAAAATCACAAGAAAATCACCCATCCAAATCCTAGAGGCGAAAGCAATCCTAGCAGGTCTGAAACATATTGTGGGTACCTGTAATCAACGCTCAATCCCATTGGAAGTAGAATCCGATGCGCTGGAAGTCGTCAATGTGATCGTTGGAGCCGTTGAAGACTTGTCAGATCTAAAACCCATCACAAACCAAATCTCTGACATAGCAACAAACTCGCACGAAATCACCTTCCGCCATTGGAACAGACTTTTGAACACAGTGGCGCACTGTGTTGCGAAGAATGCCGCGATCAATCCCTCCATTGTCGATGGCCTTGAGGAGTCCCAGGCGTCTGGTTTTTCACGAGAATGGGGTTTTGTCTTTTGGGCTCCTTTTTGCCCCCATTGGATTTTCCCTCTATTAAATGAGGGCCGATGTAATTCGGTTGATTTTCATTAA

mRNA sequence

ATGCAAAGCTTTTTACCTCAAGACTCAAGCCTCAAAAAGGCCAGGGAACCACACGAGTCAGCCTCGTGGAACGAAACCTTGGGGCGCGGAGGTCTAGGTTGGATCGTGCGTGACTCGGATGGATCTTTGGTCTATTTCGGAATGCAAAAAATCACAAGAAAATCACCCATCCAAATCCTAGAGGCGAAAGCAATCCTAGCAGGTCTGAAACATATTGTGGGTACCTGTAATCAACGCTCAATCCCATTGGAAGTAGAATCCGATGCGCTGGAAGTCGTCAATGTGATCGTTGGAGCCGTTGAAGACTTGTCAGATCTAAAACCCATCACAAACCAAATCTCTGACATAGCAACAAACTCGCACGAAATCACCTTCCGCCATTGGAACAGACTTTTGAACACAGTGGCGCACTGTGTTGCGAAGAATGCCGCGATCAATCCCTCCATTGTCGATGGCCTTGAGGAGTCCCAGGCGTCTGGTTTTTCACGAGAATGGGGTTTTGTCTTTTGGGCTCCTTTTTGCCCCCATTGGATTTTCCCTCTATTAAATGAGGGCCGATGTAATTCGGTTGATTTTCATTAA

Coding sequence (CDS)

ATGCAAAGCTTTTTACCTCAAGACTCAAGCCTCAAAAAGGCCAGGGAACCACACGAGTCAGCCTCGTGGAACGAAACCTTGGGGCGCGGAGGTCTAGGTTGGATCGTGCGTGACTCGGATGGATCTTTGGTCTATTTCGGAATGCAAAAAATCACAAGAAAATCACCCATCCAAATCCTAGAGGCGAAAGCAATCCTAGCAGGTCTGAAACATATTGTGGGTACCTGTAATCAACGCTCAATCCCATTGGAAGTAGAATCCGATGCGCTGGAAGTCGTCAATGTGATCGTTGGAGCCGTTGAAGACTTGTCAGATCTAAAACCCATCACAAACCAAATCTCTGACATAGCAACAAACTCGCACGAAATCACCTTCCGCCATTGGAACAGACTTTTGAACACAGTGGCGCACTGTGTTGCGAAGAATGCCGCGATCAATCCCTCCATTGTCGATGGCCTTGAGGAGTCCCAGGCGTCTGGTTTTTCACGAGAATGGGGTTTTGTCTTTTGGGCTCCTTTTTGCCCCCATTGGATTTTCCCTCTATTAAATGAGGGCCGATGTAATTCGGTTGATTTTCATTAA

Protein sequence

MQSFLPQDSSLKKAREPHESASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRSIPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVAKNAAINPSIVDGLEESQASGFSREWGFVFWAPFCPHWIFPLLNEGRCNSVDFH

Homology

[Close]

BLAST of Lcy09g009560 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.7e-10
Identity = 40/125 (32.00%), Postives = 72/125 (57.60%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRS 80
           ASW+++  RGG+GWI+R  DG +V  G + +   + +++LEA AIL GL+++      R 
Sbjct: 85  ASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLR- 144

Query: 81  IPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVA 140
            PL +E+D+ EV +++    EDL+    +  +I ++  +   + F    R  N  AH +A
Sbjct: 145 -PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLA 204

Query: 141 KNAAI 146
           + A++
Sbjct: 205 QRASV 207

[Close]

BLAST of Lcy09g009560 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.7e-08
Identity = 43/135 (31.85%), Postives = 71/135 (52.59%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRS 80
           A+W+   G GGLGWI+R+    +   G Q IT    I  LE  AI  G++ +V   +  S
Sbjct: 19  AAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAVV---SSSS 78

Query: 81  IPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVA 140
           + L +ES++LE +++I G  ++++++  +   I +    +    F+H  R  N+VA  +A
Sbjct: 79  VSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQEIA 138

Query: 141 KNAAINPSIVDGLEE 156
             AA      DGL E
Sbjct: 139 NRAA------DGLVE 144

[Close]

BLAST of Lcy09g009560 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.7e-07
Identity = 37/127 (29.13%), Postives = 67/127 (52.76%), Query Frame = 0


Query: 20  SASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQR 79
           +A+W      GG+GWI+RD  G ++    + I  +  I  LE  AI  GL+ I     + 
Sbjct: 92  NAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAI---RQEH 151

Query: 80  SIPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCV 139
             P+ +ESD+LE ++++    +D +++  +  +I  +  +   ++ RH +R  N VAH +
Sbjct: 152 CRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGL 211

Query: 140 AKNAAIN 147
           A+ A  N
Sbjct: 212 ARRAMEN 215

[Close]

BLAST of Lcy09g009560 vs. ExPASy TrEMBL
Match: A0A6J0LJZ5 (uncharacterized protein LOC108831539 OS=Raphanus sativus OX=3726 GN=LOC108831539 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.8e-07
Identity = 40/129 (31.01%), Postives = 67/129 (51.94%), Query Frame = 0


Query: 22  SWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRSI 81
           SW++    GG GW++RD DG L++ G +KI         EA+A+   +  ++G   +   
Sbjct: 16  SWSQQKEEGGAGWVLRDHDGRLLWAGAKKILDIGSAIETEAEALRWSMSSLLGLGYK--- 75

Query: 82  PLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVAK 141
            +  E+D+  +V ++ GA E    LKPI  ++S + +  +    R++NR  N VA  +AK
Sbjct: 76  DVMFETDSQVIVKMLQGAEEVWPRLKPIMQELSILLSGRNGFEVRYYNRSGNKVADRIAK 135

Query: 142 NAAINPSIV 151
                 SIV
Sbjct: 136 ETDTFTSIV 141

[Close]

BLAST of Lcy09g009560 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 6.3e-07
Identity = 37/131 (28.24%), Postives = 66/131 (50.38%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLK-----HIVGT 80
           A+W      GG+GWI+RD  G ++    + I  +  I  LE  AI  GL+     H    
Sbjct: 93  AAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPI 152

Query: 81  CNQRSIPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTV 140
             +   P+ +ESD+LE ++++    +D +++  +  +I  +  +   ++ RH +R  N V
Sbjct: 153 QQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKV 212

Query: 141 AHCVAKNAAIN 147
           AH +A+ A  N
Sbjct: 213 AHDLARRAMEN 223

[Close]

BLAST of Lcy09g009560 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 74.7 bits (182), Expect = 9.7e-10
Identity = 40/125 (32.00%), Postives = 72/125 (57.60%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRS 80
           ASW+++  RGG+GWI+R  DG +V  G + +   + +++LEA AIL GL+++      R 
Sbjct: 85  ASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLR- 144

Query: 81  IPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVA 140
            PL +E+D+ EV +++    EDL+    +  +I ++  +   + F    R  N  AH +A
Sbjct: 145 -PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLA 204

Query: 141 KNAAI 146
           + A++
Sbjct: 205 QRASV 207

[Close]

BLAST of Lcy09g009560 vs. NCBI nr
Match: XP_022148737.1 (uncharacterized protein LOC111017329 [Momordica charantia])

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-07
Identity = 43/135 (31.85%), Postives = 71/135 (52.59%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRS 80
           A+W+   G GGLGWI+R+    +   G Q IT    I  LE  AI  G++ +V   +  S
Sbjct: 19  AAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAVV---SSSS 78

Query: 81  IPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVA 140
           + L +ES++LE +++I G  ++++++  +   I +    +    F+H  R  N+VA  +A
Sbjct: 79  VSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQEIA 138

Query: 141 KNAAINPSIVDGLEE 156
             AA      DGL E
Sbjct: 139 NRAA------DGLVE 144

[Close]

BLAST of Lcy09g009560 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 66.2 bits (160), Expect = 3.4e-07
Identity = 37/127 (29.13%), Postives = 67/127 (52.76%), Query Frame = 0


Query: 20  SASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQR 79
           +A+W      GG+GWI+RD  G ++    + I  +  I  LE  AI  GL+ I     + 
Sbjct: 92  NAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAI---RQEH 151

Query: 80  SIPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCV 139
             P+ +ESD+LE ++++    +D +++  +  +I  +  +   ++ RH +R  N VAH +
Sbjct: 152 CRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGL 211

Query: 140 AKNAAIN 147
           A+ A  N
Sbjct: 212 ARRAMEN 215

[Close]

BLAST of Lcy09g009560 vs. NCBI nr
Match: XP_018460570.1 (PREDICTED: uncharacterized protein LOC108831539 [Raphanus sativus])

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-06
Identity = 40/129 (31.01%), Postives = 67/129 (51.94%), Query Frame = 0


Query: 22  SWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRSI 81
           SW++    GG GW++RD DG L++ G +KI         EA+A+   +  ++G   +   
Sbjct: 16  SWSQQKEEGGAGWVLRDHDGRLLWAGAKKILDIGSAIETEAEALRWSMSSLLGLGYK--- 75

Query: 82  PLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCVAK 141
            +  E+D+  +V ++ GA E    LKPI  ++S + +  +    R++NR  N VA  +AK
Sbjct: 76  DVMFETDSQVIVKMLQGAEEVWPRLKPIMQELSILLSGRNGFEVRYYNRSGNKVADRIAK 135

Query: 142 NAAINPSIV 151
                 SIV
Sbjct: 136 ETDTFTSIV 141

[Close]

BLAST of Lcy09g009560 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-06
Identity = 37/131 (28.24%), Postives = 66/131 (50.38%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLK-----HIVGT 80
           A+W      GG+GWI+RD  G ++    + I  +  I  LE  AI  GL+     H    
Sbjct: 93  AAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRPI 152

Query: 81  CNQRSIPLEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTV 140
             +   P+ +ESD+LE ++++    +D +++  +  +I  +  +   ++ RH +R  N V
Sbjct: 153 QQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISREANKV 212

Query: 141 AHCVAKNAAIN 147
           AH +A+ A  N
Sbjct: 213 AHDLARRAMEN 223

[Close]

BLAST of Lcy09g009560 vs. TAIR 10
Match: AT5G44470.1 (BEST Arabidopsis thaliana protein match is: Polynucleotidyl transferase, ribonuclease H-like superfamily protein (TAIR:AT3G23320.1); Has 74 Blast hits to 74 proteins in 2 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 45.4 bits (106), Expect = 5.9e-05
Identity = 22/68 (32.35%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 31 GLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRSIPLEVESDAL 90
          GLGWI+R+S G+ +  GM K   ++P++  E  A++  L+    + N   I +E + D L
Sbjct: 33 GLGWIIRNSHGTFMECGMSKFQGRAPVEESECTALIWALQ---SSWNLGYIKIEFDGDNL 92

Query: 91 EVVNVIVG 99
           ++ +I G
Sbjct: 93 SIIRLING 97

[Close]

BLAST of Lcy09g009560 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 3.8e-04
Identity = 34/124 (27.42%), Postives = 62/124 (50.00%), Query Frame = 0


Query: 21  ASWNETLGRGGLGWIVRDSDGSLVYFGMQKITRKSPIQILEAKAILAGLKHIVGTCNQRS 80
           A+W     R G+GWI+R+  G +++ G + + R     +LEA+  L  L+  V T ++ +
Sbjct: 151 ATWQLENPRCGIGWILRNESGGVLWMGARALPRTK--NVLEAE--LEALRWAVLTMSRFN 210

Query: 81  IP-LEVESDALEVVNVIVGAVEDLSDLKPITNQISDIATNSHEITFRHWNRLLNTVAHCV 140
              +  ESDA  +VN ++ + +    L+P    I  +  +  E+ F    R  N VA  +
Sbjct: 211 YKRIIFESDAQALVN-LLNSDDFWPTLQPALEDIQQLLHHFEEVKFEFTPRGGNKVADRI 269

Query: 141 AKNA 144
           A+ +
Sbjct: 271 ARES 269

The following BLAST results are available for this feature:

BLAST of Lcy09g009560 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy09g009560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	4.7e-10	32.00	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1D5W1	5.7e-08	31.85	uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1CP26	1.7e-07	29.13	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J0LJZ5	4.8e-07	31.01	uncharacterized protein LOC108831539 OS=Raphanus sativus OX=3726 GN=LOC108831539...	[more]
A0A6J1DSV1	6.3e-07	28.24	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Lcy09g009560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	9.7e-10	32.00	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022148737.1	1.2e-07	31.85	uncharacterized protein LOC111017329 [Momordica charantia]	[more]
XP_022143535.1	3.4e-07	29.13	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_018460570.1	1.0e-06	31.01	PREDICTED: uncharacterized protein LOC108831539 [Raphanus sativus]	[more]
XP_022156777.1	1.3e-06	28.24	uncharacterized protein LOC111023608 [Momordica charantia]	[more]

BLAST of Lcy09g009560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44470.1	5.9e-05	32.35	BEST Arabidopsis thaliana protein match is: Polynucleotidyl transferase, ribonuc...	[more]
AT2G34320.1	3.8e-04	27.42	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 20..148 e-value: 3.9E-12 score: 48.3
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 21..143 e-value: 7.0E-19 score: 67.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 21..144
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 21..144
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 21..141 e-value: 1.01228E-15 score: 68.1096
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 22..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g009560.1	Lcy09g009560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity