Lcy09g008260 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g008260
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionProlyl-tRNA synthetase
LocationChr09: 7609791 .. 7615675 (+)
RNA-Seq ExpressionLcy09g008260
SyntenyLcy09g008260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCTGTCTTGTGCTGTGCTCTACCCTCAAACTTCTTCTCGTGTTTCATCACTGTCTGCGCCTCCTCGCACTTGCGCATCGCCGCCGCGGGGTAGTTCGGTGTTCATCGAAATAGCAGAGGTTGATCCATTGTAAAGTTCATTGTAGCTCTTTTGTTACTTCAATTGCACAAGTCCCTTTTCTCTCTCTTTCTCTTTCTCTCTGACAAGCGCACAACCTCCATTTAGTGGGTCTGAAGTATGTTGAGCATCTTGGTTTACTCTTCAATGCTCGCTGTGTTCTTTTCAAATTCCTTAATCTGAGCCTCGACAAAGTCATATCTTTTATGTTCTTTTTGTTTTAATTTTATTATTTTTCTCCGTTTTTGCTATTTTGTTGCTGGGAAGGATATCAAATCTGAAAACTATTGTTCTTTTTGATAGAAATCCTTAGCTATTCAGATCTAGTCCATGAGTTCTGCCCTGTTATTTAGCTACACTTTGTTTGTTTGTTTGTTTTTTTTTTTTTTTGTTGCTTTTCCTTGCCGGGCGGGTTTGTATGGCTTGATGATTTGCGTTTCCTTGTTTATTTTCTGGCATTTGTGGGAAAAATTGACAACCGGACTCAACCCAATTCAAATTTCGTAGGGTTGACTTTAGATTCCTTCTTGTGTTCTGCTCATTCTTACATGATAGATTGTTACAACTTCTCCCAATGCCTATTTGATTATTATTTTTATTGCATTTCTTTGTTTACAATTGAATTTCTTACGATGATTCTCCTGCAGAAGTTAAAAACTGAAAACATGGCTGGTCCTAAGCCTGGTAGCTCTGCTACTAATCCAAAAGCTGGTAAATATTGTTCCTTTAATACGGTAGTTGTTTGTGTAATTGTTTCCTATTCCCTTGTTTTGTTTGTGTTTACTAGTTCTCTGCTGTTTACTGGGCAGGCGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGTTTGGGTCTCACTAATAAGAAGGATGATAACTTTGGAGAGTGGTATTCCGAGGTGCTGTTTCTTGCTTGATTTTAGTGTTCTTCATTGCAAAATGACTTTTTGTGTTGGGTAAAATTATTCATATGAGTTTCTGTTCTCATCACACTTTTACGTGACAAGGCTTGAGCTGTTTTCCTGTAACCTTGGAGAAAAATGTTTATCTTTTGTTATTGATTTCTAGAATTTCTGCATCGCTTTTTGATATATATACGTACATATTTTGGAGGAAGCTCCCTAGTAATAATATGAACAGGTTCTTTTCAGTGACTTGAATAATTTCTTTTCTAGTTTGTCTAGGTGGTTGTCAGTGGAGAAATGATTGAGTATTATGATATCTCTGGCTGCTATATACTGAGGCCGTGGGCAATGTCTATCTGGGAGACTATGCAAGTAAGGATGCTACTCTTTCTATTGTCAATTTTTTTATATGGCTCCTGTTCAAATCACATTTTTTCTTGGTATTTTGTTTTATCCTGACAATGGCTTCTAAAAATACCTGTCATTTTCTTTACAAGGTTCAGAAGTTTCTCTGGTTCTAGTAATAATTATTTGGCACCATTGAATTTTAAAATCCGTTCTTGTTCTGAAGTTTGAAGATTTAATTTGTCATTGGTGCAGGTATTTTTTGATGCAGAAATTAAGAAAATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCCGGTGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTGATGTTTTATTGTTCATATATGCAACAATAGAGAAGCTTCTCCATCTGAATTTGAAATGCTTATTTTCTTTGTTTGTTTGTTTGATATTTTAAGTTTAATATTTTTATTTATTTATGAGAAATGGGTATTTATTGAAATGCTTACTATCTTTGTTGTGGAGAATGGACATAATACCAGTTACGTTGGTTGTCATGGTCATTGGCTGATGAACTTCATTTCAATTTTCATGATATTTTGATTATCCTTAGTGCCTCTCAGTGTATCGGTTCTATGTCCTCAGTTTGTTCTTCTAGCCTCTAAACTGTTTCTTATCAGCGTAGCTTTCGCTCACGTGATGAACTTTTATTGTCCCTTATTAAAAAGTATTCTCGACAAGATTTTAGACACTCACCCCCTTCTTAGACTTTTATTCTAAGAAGTCTTTCCATTCCTTCTGTTTTGTTCATTTATTTTTGATGTCTAGGTTGATAAAAGTGCAATTGGGGGTTAGTTCTGACAATTTGATATTTGGCATTGATTTGAAGGTTGCTTGGGTAACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCAATTCGACCTACAAGTGAGACTGTGATGTACCCATACTATTCTAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAATTCAGCCATCCCACACCATTTATTAGGTAATTACTCTTGCTTGTGCTTATATTTTTGTTCCTTTTCTTTTCACCATGTTGATGGACTATCTTGGACCTGTTAACAGAGAGATATTTTATTTATCAAAAAAAAAACAGAGATATTTTATAAGTCAGTATAATCTTCTTGAACCTTTTAAGAAATTTAAAGAACTTTATTGTTTTGAAATTATTTTAAACCATTTAGAGAAAAGGACTGATCTTGTTAACTACTGGCTGCAGGAGCCGTGAGTTCCTTTGGCAGGAAGGCCATACTGCTTTTGCTACCAAGGATGAAGCAGATACTGAGGTATCAAACTCGAATTTTAAATTATGATTTAGAAAAATGCTTTTGTCATGGTTGGCTTTAGTGTGTGAAACCAACTTGTCTTTTTCTTAGTTTCTAATGATGAAAATAAGTCAAATATTTGTGGGATTAGTGGTTTGGGAGGAAACTTAGAATCTTTAAATGGGAAGCGTACAGATCTGATGAATTGAATCTCTCGTTGTTATTTAGTTTCTCTTTTGGGTTGACTTTTTGTGTAAAAATAAAGAAGTTAGTAGATTTTTGCATCCCTTTCATCTGCAATTTCTACCTCAATAATAATTAAATTTTTAAGAACATAATAAAGTCGTTGATTTGTTTGATGTCACAAGAAAGTGTTGTGGAGTCTTAAGATAAGGTAACTGTGAACATGTGGACTTTTTTAGTACTTTTGTGATATAAGGTCACCGTAGCACCAACGGAATAATTATAAATTTTGTTTATCTTGAGCAAATTTGGAGATTCAACTATGCAAACTCACTTTGATTTGAAGCAAAAAGAATGAGTGGAGTCTAAATTGGCAACATCAAGTTAATCAAGTGATCATCCTCAAGCATTTTGAACTCAAGGATGAGTTTTCTTCAGTCGGAAAAGCATGATGGTGTGATTTAGATATTAATTGTCTCATAAATTTTAGATTTTGTATATTATTGGATAACTTTTAATAGTCAAAACTGGATTTTTCTTTGAAAGTTTGCAGATTATTTGTGAATTCAATTACATTTTATCAACTATTGGATAATAGATGTTATGCTTCTGATTTCTCTCTTCTTTTTCCTTTGGGTAGAAAACAAAGTTGTATAATTAATTGAAAGAATGTAAAGGGGAGAGGGAAGCTTAAATCTTCATCTTATTTTTAACCAATCCAAGAGAGATTTCAAAAACACGGTTCAATCAGATCCTGAAAATATGCAGTTTTTTTGTGTAACTGTGGTCATTATTTAAGGGTTACTTTTATTTCAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATATCTTGCAATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAGAAGTTCGCTGGTGGTCTTTACACCACAAGTGTTGAGGTATAAATTGACAAATGTTATGACCTGTTTTCAGCATGGTGAATCAATATCCGTTACTGGTTTAAGTTGTATAAAACCATTTCTTCATGTTTGATATGCAGGCGTTTATCCCAAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGTTTGGGTCAAAACTTTGCAAAAATGTTTGAAATAAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACTAGAACGGTAATACTTCGATATTTGCATTTGTCTTTGGTAACTTGTCACAGTGAAATTCTGGGTTCCTTAAATAAAATAGTGATGAGAGCACATATTACCTTTTATTAATGCTCAATTCTTTTAGTTGTTTATCCTGTCTTTCCAAATTGTTTGCAGATTGGTGTGATGGTTATGGTTCACGGGGACGACAAGGGATTGGTGCTGCCTCCTAAAGTTGCATCAGTCCAAGTTGTTATAGTTCCTGTTCCTTACAAAGATGCAGATACACAAGGAATTCTTGATGCATGTTCTGCCACTTTGGATACATTGTCTGAAGCAGGAATTCGTGCAGAGGTGGACTCCAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAGGGTGTTCCACTCAGGATTGAAATAGGACCTAAGGATTTGGCAAACAATCAGGTTACATATTCTGTCTATTAGTGTCTTTTTCATCACTACTGAAATACTGTTTCCCCTCGCTAACACTTTTACAACTCTTAATGACTTGGATTGTGTTGATACGTAGTGCTAATACATATTTATATACTTTATTTCTAGGTACGTGCTGTTCGCCGTGATAATTCGAGTAAAAAGGACATACCTAGGGATTCATTGGTTGAACAAGTGAAAGAATTGCTAGAAAGTATTCAACAAAGCCTGTTTGACAAGGCAAAAGAAAAAAGAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGTATAGTTTGTGTTCTTATTGCTATCTTGATTCTGCTTGTGAATGGTTGACTGCTTTTTGGTTCTGACTTTTCATGAGGCCACCATAATTTTATTTGCTTAGTTGAAAGCAGATATGGTTATTGATTTTCAATATACAATCCACTTTCATCTTCTGGAATCTATATGCATAATCAACTGTAAATTCCTGGTTTGTTGTTGAATTTGTTTTTGTGGAGCTTGTTTACGAGCTAGGAAGAAATTGTTGCTCGTTTTGGCCGCCATCAGGATAATTTCTGTGGAATTGAGGGGGTGATGTCACTAATATTTTTAACTCCATCGAGCACTGGCTTGAGTAAATGCATTTCAAGTGTTTAGTTTCTAATGAAGATAGTGTTAGTTTCTGAAATATGAAAATTGTGAAATATTTGTTCTATGCCATCCTTTATCATCCACTGGTGTACCTTTTTTCCTCATGATGGTTCCATTGTAATGATCCTCTAGGAGGTTGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCCAAAACCTTATGTTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACTAAACTAACTTCGTTCTAAATCCGTATTCTTTTTTTCGACAACAGGAATGGAATTTATATTATCTTGTGACATCTGGGGAAATTTATTAGCAACTCTTTTTGTTTCCTTGAATTCTTGTGATGTTTGTCATCAGGTACCAAATGTTTTGCATCTGGAAAGCCTGCAAAGAAATGGAGCTATTGGGGGCGAAGCTACTAAGTTACAAGCGCACCGAGAAATCGGCTTTAGAGTTCTTGAGTACGAGGGCATTCGATTCGGTTAATATCCCGAGTTCATCAGAATTTGCAACAGTCACTCTTATTCAATGAAGAAAAGCATTCACCAAGAAAAGTTAACTATAGAACAATCTTTTACTCTGTTTGCATACCTGGATTTAAATTGTTTTTTTTTTTTTTTTTTTTTTT

mRNA sequence

TCCCTGTCTTGTGCTGTGCTCTACCCTCAAACTTCTTCTCGTGTTTCATCACTGTCTGCGCCTCCTCGCACTTGCGCATCGCCGCCGCGGGGTAGTTCGGTGTTCATCGAAATAGCAGAGAAGTTAAAAACTGAAAACATGGCTGGTCCTAAGCCTGGTAGCTCTGCTACTAATCCAAAAGCTGGCGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGTTTGGGTCTCACTAATAAGAAGGATGATAACTTTGGAGAGTGGTATTCCGAGGTGGTTGTCAGTGGAGAAATGATTGAGTATTATGATATCTCTGGCTGCTATATACTGAGGCCGTGGGCAATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCAGAAATTAAGAAAATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCCGGTGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTTGCTTGGGTAACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCAATTCGACCTACAAGTGAGACTGTGATGTACCCATACTATTCTAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAATTCAGCCATCCCACACCATTTATTAGGAGCCGTGAGTTCCTTTGGCAGGAAGGCCATACTGCTTTTGCTACCAAGGATGAAGCAGATACTGAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATATCTTGCAATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAGAAGTTCGCTGGTGGTCTTTACACCACAAGTGTTGAGGCGTTTATCCCAAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGTTTGGGTCAAAACTTTGCAAAAATGTTTGAAATAAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCACGGGGACGACAAGGGATTGGTGCTGCCTCCTAAAGTTGCATCAGTCCAAGTTGTTATAGTTCCTGTTCCTTACAAAGATGCAGATACACAAGGAATTCTTGATGCATGTTCTGCCACTTTGGATACATTGTCTGAAGCAGGAATTCGTGCAGAGGTGGACTCCAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAGGGTGTTCCACTCAGGATTGAAATAGGACCTAAGGATTTGGCAAACAATCAGGTACGTGCTGTTCGCCGTGATAATTCGAGTAAAAAGGACATACCTAGGGATTCATTGGTTGAACAAGTGAAAGAATTGCTAGAAAGTATTCAACAAAGCCTGTTTGACAAGGCAAAAGAAAAAAGAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGAGGTTGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCCAAAACCTTATGTTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACCAAATGTTTTGCATCTGGAAAGCCTGCAAAGAAATGGAGCTATTGGGGGCGAAGCTACTAAGTTACAAGCGCACCGAGAAATCGGCTTTAGAGTTCTTGAGTACGAGGGCATTCGATTCGGTTAATATCCCGAGTTCATCAGAATTTGCAACAGTCACTCTTATTCAATGAAGAAAAGCATTCACCAAGAAAAGTTAACTATAGAACAATCTTTTACTCTGTTTGCATACCTGGATTTAAATTGTTTTTTTTTTTTTTTTTTTTTTT

Coding sequence (CDS)

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCTACTAATCCAAAAGCTGGCGGGAAAAAGAAGGAGGTGAAGAAAGAGACTGGTTTGGGTCTCACTAATAAGAAGGATGATAACTTTGGAGAGTGGTATTCCGAGGTGGTTGTCAGTGGAGAAATGATTGAGTATTATGATATCTCTGGCTGCTATATACTGAGGCCGTGGGCAATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCAGAAATTAAGAAAATGAAAATCAAGAACTGCTATTTTCCACTTTTTGTATCTCCCGGTGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTTGCTTGGGTAACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCAATTCGACCTACAAGTGAGACTGTGATGTACCCATACTATTCTAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAATTCAGCCATCCCACACCATTTATTAGGAGCCGTGAGTTCCTTTGGCAGGAAGGCCATACTGCTTTTGCTACCAAGGATGAAGCAGATACTGAGGTTCTTGAGATATTGGAACTGTATAGGCGCATATATGAAGAATATCTTGCAATTCCTGTTATCAAGGGCAAGAAGAGTGAGATGGAGAAGTTCGCTGGTGGTCTTTACACCACAAGTGTTGAGGCGTTTATCCCAAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGTTTGGGTCAAAACTTTGCAAAAATGTTTGAAATAAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCCTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCACGGGGACGACAAGGGATTGGTGCTGCCTCCTAAAGTTGCATCAGTCCAAGTTGTTATAGTTCCTGTTCCTTACAAAGATGCAGATACACAAGGAATTCTTGATGCATGTTCTGCCACTTTGGATACATTGTCTGAAGCAGGAATTCGTGCAGAGGTGGACTCCAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAGGGTGTTCCACTCAGGATTGAAATAGGACCTAAGGATTTGGCAAACAATCAGGTACGTGCTGTTCGCCGTGATAATTCGAGTAAAAAGGACATACCTAGGGATTCATTGGTTGAACAAGTGAAAGAATTGCTAGAAAGTATTCAACAAAGCCTGTTTGACAAGGCAAAAGAAAAAAGAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGAGGTTGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCCAAAACCTTATGTTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACCAAATGTTTTGCATCTGGAAAGCCTGCAAAGAAATGGAGCTATTGGGGGCGAAGCTACTAA

Protein sequence

MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSLFDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Homology
BLAST of Lcy09g008260 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 8.5e-258
Identity = 418/497 (84.10%), Postives = 459/497 (92.35%), Query Frame = 0

Query: 15  AGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETM 74
           +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE M
Sbjct: 35  SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIM 94

Query: 75  QVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRP 134
           Q+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRP
Sbjct: 95  QIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRP 154

Query: 135 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKD 194
           TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK 
Sbjct: 155 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKA 214

Query: 195 EADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGAT 254
           EAD EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGAT
Sbjct: 215 EADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGAT 274

Query: 255 SHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 314
           SHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASV
Sbjct: 275 SHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASV 334

Query: 315 QVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLR 374
           QVV++PVPYKDA+TQGI DAC+AT   L EAGIRAE D RDNYSPGWKYS WEMKGVPLR
Sbjct: 335 QVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLR 394

Query: 375 IEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSLFDKAKEKRDACIQV 434
           IEIGP+DL N+QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK+KR+AC+Q 
Sbjct: 395 IEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQE 454

Query: 435 INTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKC 494
           + TW+EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT C
Sbjct: 455 VKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLC 514

Query: 495 FASGKPAKKWSYWGRSY 512
           FASGKPAKKW+YWGRSY
Sbjct: 515 FASGKPAKKWTYWGRSY 530

BLAST of Lcy09g008260 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.5e-164
Identity = 297/546 (54.40%), Postives = 371/546 (67.95%), Query Frame = 0

Query: 2   AGPKPGSSATNPKAGGKKKEVKKETG-------LGLTNKKDDNFGEWYSEVVVSGEMIEY 61
           + P   ++ + P A  KK E  K          +G+T +KD +F  WY +V+   +MIEY
Sbjct: 172 SAPGSATAPSKPAAQKKKAEPSKNDAAIENAALIGITVRKDADFPNWYQQVLTKSDMIEY 231

Query: 62  YDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE 121
           YDISGCYIL+PW+ SIWE +Q +FD EIKK+ ++N YFPLFVS  VL++EKDH+EGFAPE
Sbjct: 232 YDISGCYILKPWSYSIWEAIQGWFDKEIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPE 291

Query: 122 VAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTP 181
           VAWVT++G S+L+ PIAIRPTSETVMYPYY+KWIR HRDLPLKLNQW +VVRWEF +P P
Sbjct: 292 VAWVTRAGTSELDEPIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKNPQP 351

Query: 182 FIRSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGL 241
           F+R+REFLWQEGHTA  T + A  EV +IL+LY RIY + LA+PVIKG KSE EKFAGG+
Sbjct: 352 FLRTREFLWQEGHTAHMTLEGATEEVHQILDLYARIYTDLLAVPVIKGVKSENEKFAGGM 411

Query: 242 YTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------KAMVWQNSWA 301
           +TT+VE +IP TGRGIQGATSHCLGQNF+KMF I  E+   E        K  VWQNSW 
Sbjct: 412 FTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSWG 471

Query: 302 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVVIVPV----PYKDADTQGILDACSATLDTL 361
            STRTIGV VMVHGDDKGL LPP +A VQ V+VP        D +   I   CS   D L
Sbjct: 472 LSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEGFCSKLADRL 531

Query: 362 SEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPR 421
           + A IR E D R  Y+PG+K+SHWEMKGVPLR+E GP D   NQV AVRRD   K  +P 
Sbjct: 532 NAADIRTEADLR-AYTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFEKIPVPL 591

Query: 422 DSLVEQVKELLESIQQSLFDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVE 481
           ++L + V +LL  IQ ++++ AK +RDA +  +  W +F  AL++K +++ PWC+  E E
Sbjct: 592 NNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMIPWCNTTECE 651

Query: 482 KDVKTRT------------KGEMGAAKTLCSPFEQP----ALPEG-TKCFASGKPAKKWS 512
           K++K  +            K     AK+LC P EQP    A+ EG TKC   G  AK W 
Sbjct: 652 KEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAGCGNLAKVWG 711

BLAST of Lcy09g008260 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 578.2 bits (1489), Expect = 9.4e-164
Identity = 291/527 (55.22%), Postives = 374/527 (70.97%), Query Frame = 0

Query: 2    AGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 61
            A PKP        A      VKK+T LGL   K+DN  +WYS+V+  GEMIEYYD+SGCY
Sbjct: 1189 AQPKPAKPVKKEPAADASGAVKKQTRLGLEATKEDNLPDWYSQVITKGEMIEYYDVSGCY 1248

Query: 62   ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 121
            ILR W+ +IW+ ++ +FDAEI +M +K CYFP+FVS  VL++EK HI  FAPEVAWVTKS
Sbjct: 1249 ILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVLEKEKTHIADFAPEVAWVTKS 1308

Query: 122  GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 181
            G+SDL  PIA+RPTSETVMYP Y+KW++ +RDLP++LNQW NVVRWEF  PTPF+R+REF
Sbjct: 1309 GDSDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVRWEFKQPTPFLRTREF 1368

Query: 182  LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 241
            LWQEGHTAFA K+EA  EVL+IL+LY  +Y   LAIPV+KG+K+E EKFAGG YTT+VEA
Sbjct: 1369 LWQEGHTAFADKEEAAKEVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGGDYTTTVEA 1428

Query: 242  FIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNSWAYSTRTIGVMVMVHG 301
            FI  +GR IQGATSH LGQNF+KMFEI +E+ E  +K  V+QNSW  +TRTIGVM+MVH 
Sbjct: 1429 FISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTIGVMIMVHA 1488

Query: 302  DDKGLVLPPKVASVQVVIVP----VPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDN 361
            D++GLVLPP VA +Q ++VP    V  KD +   +LDAC A    L   G+R E D RDN
Sbjct: 1489 DNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRDN 1548

Query: 362  YSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESI 421
            YSPGWK++HWE+KGVPLR+E+GPKDL   Q+ AVRRD   K  IP   + +++  LLE+I
Sbjct: 1549 YSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKKIPALLETI 1608

Query: 422  QQSLFDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRT----KGE 481
             +S+ +KA+E   +  + +  W +F   L QK ++LAP+C E   E  +K  +    + E
Sbjct: 1609 HESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKADSARGEEAE 1668

Query: 482  MGA----AKTLCSPFEQPA-LPEGTKCF---ASGKPAKKWSYWGRSY 512
             GA    AK+LC PF+QPA +    KC     + KP K ++ +GRSY
Sbjct: 1669 PGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714

BLAST of Lcy09g008260 vs. ExPASy Swiss-Prot
Match: P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)

HSP 1 Score: 577.8 bits (1488), Expect = 1.2e-163
Identity = 283/510 (55.49%), Postives = 366/510 (71.76%), Query Frame = 0

Query: 17   GKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQV 76
            G+ +  KK+T LGL  KK++N  +WYS+V+   EMIEY+DISGCYILRPWA +IWE ++ 
Sbjct: 1003 GEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKD 1062

Query: 77   FFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTS 136
            FFDAEIKK+ ++NCYFP+FVS   L++EK H+  FAPEVAWVT+SG+++L  PIAIRPTS
Sbjct: 1063 FFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTS 1122

Query: 137  ETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEA 196
            ETVMYP Y+KW++ HRDLP+KLNQWCNVVRWEF HP PF+R+REFLWQEGH+AFAT +EA
Sbjct: 1123 ETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEA 1182

Query: 197  DTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSH 256
              EVL+IL+LY ++YEE LAIPV+KG+K+E EKFAGG YTT++EAFI  +GR IQG TSH
Sbjct: 1183 AEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSH 1242

Query: 257  CLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 316
             LGQNF+KMFEI FE+ K  GEK   +QNSW  +TRTIGVM MVHGD+ GLVLPP+VA V
Sbjct: 1243 HLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACV 1302

Query: 317  QVVIVPVPYKDA----DTQGILDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKG 376
            QVVI+P    +A    D + ++  C+     L    IR   D RDNYSPGWK++HWE+KG
Sbjct: 1303 QVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKG 1362

Query: 377  VPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSLFDKAKEKRDA 436
            VP+R+E+GP+D+ + Q  AVRRD   K  +  +    +++ +LE IQ +LF +A E    
Sbjct: 1363 VPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKT 1422

Query: 437  CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMGA----AKTLCSP 496
             + V NT E+F + L   K++  P+C E + E  +K  T      E GA    AK+LC P
Sbjct: 1423 HMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIP 1482

Query: 497  FEQPA-LPEGTKCFASGKPAKKWSYWGRSY 512
            F+    L  G KC     PAK ++ +GRSY
Sbjct: 1483 FKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1512

BLAST of Lcy09g008260 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 577.0 bits (1486), Expect = 2.1e-163
Identity = 290/522 (55.56%), Postives = 372/522 (71.26%), Query Frame = 0

Query: 5    KPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILR 64
            K   S  +    G+ +  KK+T LGL  KK++N  EWYS+V+   EMIEYYD+SGCYILR
Sbjct: 991  KSQGSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILR 1050

Query: 65   PWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGES 124
            PW+ SIWE+++ FFDAEIKK+ ++NCYFP+FVS   L++EK+HIE FAPEVAWVT+SG++
Sbjct: 1051 PWSYSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKT 1110

Query: 125  DLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQ 184
            +L  PIAIRPTSETVMYP Y+KW++ HRDLP++LNQWCNVVRWEF HP PF+R+REFLWQ
Sbjct: 1111 ELAEPIAIRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLRTREFLWQ 1170

Query: 185  EGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIP 244
            EGH+AFAT +EA  EVL+ILELY R+YEE LAIPV++G+K+E EKFAGG YTT++EAFI 
Sbjct: 1171 EGHSAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFIS 1230

Query: 245  NTGRGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDD 304
             +GR IQGATSH LGQNF+KM EI FE+ K  GEK   +Q SW  +TRTIGVMVMVHGD+
Sbjct: 1231 ASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDN 1290

Query: 305  KGLVLPPKVASVQVVIVPVPYKDA----DTQGILDACSATLDTLSEAGIRAEVDSRDNYS 364
             GLVLPP+VASVQVV++P    +A    D + ++  C+     L  A IR  VD RDNYS
Sbjct: 1291 MGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYS 1350

Query: 365  PGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQ 424
            PGWK++HWE+KGVP+R+E+GP+D+ + Q  AVRRD   K  I       +++++LE IQ 
Sbjct: 1351 PGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQL 1410

Query: 425  SLFDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMG 484
            +LF +A E     + V NT E+F + L   K+   P+C E + E  +K  T      E G
Sbjct: 1411 NLFTRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPG 1470

Query: 485  A----AKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 512
            A    AK+LC PF     L  G  C     PAK ++ +GRSY
Sbjct: 1471 APSMGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512

BLAST of Lcy09g008260 vs. ExPASy TrEMBL
Match: A0A5D3D175 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001250 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.1e-297
Identity = 498/511 (97.46%), Postives = 506/511 (99.02%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACSATLDTL++AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS+KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. ExPASy TrEMBL
Match: A0A1S3BXN6 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494536 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.1e-297
Identity = 498/511 (97.46%), Postives = 506/511 (99.02%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACSATLDTL++AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS+KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. ExPASy TrEMBL
Match: A0A6J1GUJ5 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 2.5e-297
Identity = 501/511 (98.04%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSAT PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACS TLDTLSEAGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR +LVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of Lcy09g008260 vs. ExPASy TrEMBL
Match: A0A0A0KTZ1 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 4.3e-297
Identity = 498/511 (97.46%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNKKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIK+MKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKQMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACSATLDTL+ AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AK KRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. ExPASy TrEMBL
Match: A0A6J1GYJ1 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 5.7e-297
Identity = 499/511 (97.65%), Postives = 502/511 (98.24%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVVIVPVPYKDADTQGI DACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS+KKDIPR SLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. NCBI nr
Match: XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.5e-299
Identity = 503/511 (98.43%), Postives = 505/511 (98.83%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYR IYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRCIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACSATLDTLSEAGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AKEKRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. NCBI nr
Match: XP_038874652.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida])

HSP 1 Score: 1031.9 bits (2667), Expect = 1.8e-297
Identity = 498/511 (97.46%), Postives = 505/511 (98.83%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSS TNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACSATLDTLS+AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR SLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AKEKRDACI+VINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDACIKVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFQQPDLPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. NCBI nr
Match: XP_008453976.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >TYK16984.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.4e-297
Identity = 498/511 (97.46%), Postives = 506/511 (99.02%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSATN KAGGKKKEVKKETGLGLTNKKD+NFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNQKAGGKKKEVKKETGLGLTNKKDENFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFEN+KGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENDKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACSATLDTL++AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTDAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS+KKDIPRDSLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. NCBI nr
Match: XP_022955643.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata] >KAG6582053.1 Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.3e-297
Identity = 501/511 (98.04%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSAT PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGI DACS TLDTLSEAGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPR +LVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AKEKRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of Lcy09g008260 vs. NCBI nr
Match: XP_023543704.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.3e-297
Identity = 500/511 (97.85%), Postives = 502/511 (98.24%), Query Frame = 0

Query: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVVIVPVPYKDADTQGI DACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS+KKDIPR SLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLESIQQSL 420

Query: 421 FDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FD AK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of Lcy09g008260 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 890.6 bits (2300), Expect = 6.1e-259
Identity = 418/497 (84.10%), Postives = 459/497 (92.35%), Query Frame = 0

Query: 15  AGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETM 74
           +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE M
Sbjct: 35  SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIM 94

Query: 75  QVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRP 134
           Q+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRP
Sbjct: 95  QIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRP 154

Query: 135 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKD 194
           TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK 
Sbjct: 155 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKA 214

Query: 195 EADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGAT 254
           EAD EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGAT
Sbjct: 215 EADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGAT 274

Query: 255 SHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 314
           SHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASV
Sbjct: 275 SHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASV 334

Query: 315 QVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLR 374
           QVV++PVPYKDA+TQGI DAC+AT   L EAGIRAE D RDNYSPGWKYS WEMKGVPLR
Sbjct: 335 QVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLR 394

Query: 375 IEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSLFDKAKEKRDACIQV 434
           IEIGP+DL N+QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK+KR+AC+Q 
Sbjct: 395 IEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQE 454

Query: 435 INTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKC 494
           + TW+EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT C
Sbjct: 455 VKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLC 514

Query: 495 FASGKPAKKWSYWGRSY 512
           FASGKPAKKW+YWGRSY
Sbjct: 515 FASGKPAKKWTYWGRSY 530

BLAST of Lcy09g008260 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 890.6 bits (2300), Expect = 6.1e-259
Identity = 418/497 (84.10%), Postives = 459/497 (92.35%), Query Frame = 0

Query: 15  AGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETM 74
           +G KKK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE M
Sbjct: 35  SGQKKKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIM 94

Query: 75  QVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRP 134
           Q+FFDAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRP
Sbjct: 95  QIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRP 154

Query: 135 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKD 194
           TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK 
Sbjct: 155 TSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKA 214

Query: 195 EADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGAT 254
           EAD EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGAT
Sbjct: 215 EADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGAT 274

Query: 255 SHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASV 314
           SHCLGQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASV
Sbjct: 275 SHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASV 334

Query: 315 QVVIVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLR 374
           QVV++PVPYKDA+TQGI DAC+AT   L EAGIRAE D RDNYSPGWKYS WEMKGVPLR
Sbjct: 335 QVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLR 394

Query: 375 IEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLESIQQSLFDKAKEKRDACIQV 434
           IEIGP+DL N+QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK+KR+AC+Q 
Sbjct: 395 IEIGPRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQE 454

Query: 435 INTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKC 494
           + TW+EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT C
Sbjct: 455 VKTWDEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLC 514

Query: 495 FASGKPAKKWSYWGRSY 512
           FASGKPAKKW+YWGRSY
Sbjct: 515 FASGKPAKKWTYWGRSY 530

BLAST of Lcy09g008260 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 422.5 bits (1085), Expect = 4.7e-118
Identity = 214/501 (42.71%), Postives = 309/501 (61.68%), Query Frame = 0

Query: 18  KKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVF 77
           K  EV +         +  +F  WY +V+ S E+ +Y  + G  ++RP+  +IWE +Q +
Sbjct: 53  KSSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQDY 112

Query: 78  FDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSE 137
            + + K+    N YFP F+    +++E  H+EGF+PE+A VT  G  +LE  + +RPTSE
Sbjct: 113 LNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSE 172

Query: 138 TVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEAD 197
           T++   +++WI  +RDLPL +NQW NV RWE     PFIR+ EFLWQEGHTA AT +EA+
Sbjct: 173 TIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFIRTLEFLWQEGHTAHATPEEAE 232

Query: 198 TEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHC 257
            E  +++E+Y R   E  AIPVI G+KS++E FAG   T ++EA + +  + +Q  TSH 
Sbjct: 233 KEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDR-KALQAGTSHN 292

Query: 258 LGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVV 317
           LGQNF++ F   F +E GE+  VWQ SWA STR +G ++M HGDD GL+LPPK+A +QVV
Sbjct: 293 LGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVV 352

Query: 318 IVPVPYKDADTQGILDACSATLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEI 377
           IVP+  KD +  G+L A S+  + L  AG+R ++D  D  +PGWK++ WEMKG+PLRIEI
Sbjct: 353 IVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEI 412

Query: 378 GPKDLANNQVRAVRRDNSSKK------DIPRDSLVEQVKELLESIQQSLFDKAKEKRDAC 437
           GP+D+++N V   RRD   K        +   +LV  VKE L+ IQ SL +KA   RD+ 
Sbjct: 413 GPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDEIQTSLLEKALSFRDSN 472

Query: 438 IQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEG 497
           I  +N++ E  +A+S  K    PW   +  E+ VK  T      A   C PFEQ    +G
Sbjct: 473 IVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEET-----GATIRCFPFEQ---TQG 532

Query: 498 TK-CFASGKPAKKWSYWGRSY 512
           TK C  +G PA++ + + +SY
Sbjct: 533 TKTCLMTGNPAEEVAIFAKSY 543

BLAST of Lcy09g008260 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 290.0 bits (741), Expect = 3.6e-78
Identity = 146/221 (66.06%), Postives = 167/221 (75.57%), Query Frame = 0

Query: 297 MVHGDDKGLVLPPKVASVQVVIVPVPYKD-ADTQGILDACSATLDTLSEAGIRAEVDSRD 356
           M HGDDKGLV PPKVA VQVV++ VP K  AD Q + DAC A   TL  AGIRAE D RD
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 357 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSSKKDIPRDSLVEQVKELLES 416
           NYS GWKY+  E+ GVPLRIE GP+DLAN+QVR V RDN +K D+ R  L+EQVK+LLE 
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208

Query: 417 IQQSLFDKAKEKRDACIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEM-- 476
           IQ +L+D AK K + C Q + TW+EF EALSQKK+ILAPWCD+ EVEKDVK RT+G+   
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETG 268

Query: 477 ---GAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 512
              G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 GGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309


HSP 2 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 27/47 (57.45%), Postives = 37/47 (78.72%), Query Frame = 0

Query: 24 KETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD-ISGCYILRPWAMS 70
          KET LG+T KKD++FG+WYSE    GE++EYY+ + GCYIL+P  +S
Sbjct: 3  KETRLGITAKKDEDFGKWYSEACRFGELVEYYESVKGCYILKPSGIS 49

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1R28.5e-25884.10Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P... [more]
O601552.5e-16454.40Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P286689.4e-16455.22Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G... [more]
P078141.2e-16355.49Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=... [more]
Q8CGC72.1e-16355.56Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE... [more]
Match NameE-valueIdentityDescription
A0A5D3D1751.1e-29797.46Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BXN61.1e-29797.46Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494536 PE=3 SV=1[more]
A0A6J1GUJ52.5e-29798.04Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1[more]
A0A0A0KTZ14.3e-29797.46Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1[more]
A0A6J1GYJ15.7e-29797.65Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038907011.12.5e-29998.43proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907... [more]
XP_038874652.11.8e-29797.46proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida][more]
XP_008453976.12.4e-29797.46PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >TYK16984... [more]
XP_022955643.15.3e-29798.04proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata] >KAG6582053.1 ... [more]
XP_023543704.15.3e-29797.85proline--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G62120.16.1e-25984.10Class II aaRS and biotin synthetases superfamily protein [more]
AT3G62120.26.1e-25984.10Class II aaRS and biotin synthetases superfamily protein [more]
AT5G52520.14.7e-11842.71Class II aaRS and biotin synthetases superfamily protein [more]
AT5G10880.13.6e-7866.06tRNA synthetase-related / tRNA ligase-related [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002316Proline-tRNA ligase, class IIaPRINTSPR01046TRNASYNTHPROcoord: 170..181
score: 42.59
coord: 160..168
score: 48.97
coord: 89..107
score: 25.15
coord: 130..141
score: 49.38
IPR016061Proline-tRNA ligase, class II, C-terminalSMARTSM00946ProRS_C_1_2coord: 438..511
e-value: 2.2E-28
score: 110.3
IPR016061Proline-tRNA ligase, class II, C-terminalPFAMPF09180ProRS-C_1coord: 438..511
e-value: 3.2E-26
score: 91.4
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 309..429
e-value: 3.8E-41
score: 141.7
IPR017449Prolyl-tRNA synthetase, class IIGENE3D3.30.110.30coord: 432..511
e-value: 2.0E-28
score: 100.4
IPR017449Prolyl-tRNA synthetase, class IISUPERFAMILY64586C-terminal domain of ProRScoord: 433..511
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 315..411
e-value: 1.9E-20
score: 72.8
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 31..308
e-value: 3.8E-110
score: 369.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR43382:SF4PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKEcoord: 13..511
NoneNo IPR availableCDDcd00862ProRS_anticodon_zinccoord: 304..511
e-value: 3.1258E-95
score: 285.731
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 303..429
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 36..302
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 130..296
e-value: 7.1E-18
score: 65.2
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeTIGRFAMTIGR00408TIGR00408coord: 32..511
e-value: 1.3E-181
score: 602.6
IPR004499Proline-tRNA ligase, class IIa, archaeal-typePANTHERPTHR43382PROLYL-TRNA SYNTHETASEcoord: 13..511
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeHAMAPMF_01571Pro_tRNA_synth_type3coord: 31..511
score: 38.623589
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 70..308
score: 18.88479
IPR033721Prolyl-tRNA synthetase, catalytic domainCDDcd00778ProRS_core_arch_eukcoord: 37..298
e-value: 6.20576E-165
score: 465.532

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g008260.1Lcy09g008260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006433 prolyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004827 proline-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding