Lcy09g008240 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g008240
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectinesterase
LocationChr09: 7601718 .. 7604151 (+)
RNA-Seq ExpressionLcy09g008240
SyntenyLcy09g008240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTGTGGTCTGTTTGAGAATGATCTTCCCAAATGAGTAGAAAAAGAACACTAAAATTAAACCACAAATCCCTCAAATTTATACCTGCATTCCTAAAAAACAGCTCCTATTTTTGGCAAACTTCACCACTATATAATCTTTCCCATGCAGACCATTTCTCATCACCTCCTCCATTCAATATCATTACAAAAATCCCAAAAAAAAATAAAAACAATACTCAAAACCCACTCTTTTTCCCCATCTTTCTGGTGGGAAAAGCTTCTCCCACCTGGGTTTTGATCATTCATTAACAGGAGGAAGGCCTTTTCCACATTACAAGAAACAGGAGGGAGCAAAAACACATAAAATAAAATGGTAGGGAAGGTGGTCGTGTCGGGAATATCCCTTATTCTCGTCGTCGGTGTAGCTCTCGCCGTCGTCGCCGTCGTTAACAAGAACAATAGTTCCGAAGCTGCGACGGAGGATCTGTCTCCAAAGATGAAGGCAGTCTCAACCATTTGTTCTGGTGCTGATTACCGTAAAGAATGCCAAACTACTCTTTCCAGCGTTGCTCAAAATACCTCTTCAGATGACCCCAAGGAGTATCTTAAAGCGGCAGTTCTTGCCACTATAGATGAGATTAAGAAAGGCTACAACTTGTCCGATAGCTTTCTAGTCGAGCCTATTAACAATGCCACCATCAAAATGTCCATTGAGGATTGCAGGGACTTACTGCAGTTCGCCATCGACGAACTGCAGGCGTCTGCCTCCGCTGTCGGGGACCCCACAATGCACTCTGAAGCAGACCGCGCTGCCGATATCAGGAATTGGCTCAGCGCCGTCGTTTCTTATCAGCAATCTTGCCTCGACGGACTCGAAGAAACCGACCCCCAGCTCAAACAGAAGATGCAAGACGGCCTAGATGGTGCTGGCAAGCTTACTAGCAATGCTCTTGCCGTTCTCGACGCAGTTACCGAAATTCTCGAAAGCCTGGGCTTGCAGTTGAAGGTTCTGCTAAATTACTTACGAACCTCCAAAAGTTTAAGTTGATAGGTTATGATATAATATATTTTCTTTGTTAGCAGGTCCAACCAACTGGCCGTAGGTTGCTTGGAACAACAGAAGTAGGGAGCGACGGGTTCCCGACGTGGTTATCGGCGGCAGATAGAAAGCTGCTGGCAGCCAGAGGAGGAGGAGCCCCCCGAGTGAGGCCGAACGCAGTGGTGGCGAAGGACGGTAGCGGGCAGTTCAAAACCATTGCTGCTGCATTGGCTGCATATCCAAAGGTTCCAAGAGGAAGATATATAATCTACGTGAAAGCAGGGATCTATGATGAGTACATCACTGTGGATCAAAACATGAAGAATGTTTTCATGTATGGTGATGGCCCAAGAAAGACCATTGTCACTGGCCGCAAGTGCAATCGTGATGGCATCACCACCCAAAACACTGCCTCTTTCTGTAAGTAACTCCGACTCTGTCTCCGAAATTTTCCATACATTTCCGAAAGCATACCTTCTAACTTTTTATATATATAATCTTTCGAGTTCTTCAAATGTATCTAACTTCAATTCAGCAAAATTCCATCTAAATACCTAAATTGTTGAAAAGAAACTAACACATTACATTGCAAAATTACCTTGGCAGCTGCCATAGGAGACGATTTCCTGTGCAAATCGATGGGATTCCAAAACACAGCCGGCCCCCAAGGGCACCAAGCCGTAGCCCTAAGAGTCCAATCCGACAGGTCGGCGTTCTTCAACTGCAGAATGGACGGCTACCAAGACACCCTGTACGTGCAAACCCATCGTCAATTCTACCGCAACTGCGTGATCTCCGGCACCGTGGACTTCATCTTCGGCGATTCAGCGACGATCATCCAGAACTCGTTGATCATCGTCCGACGCCCAATGGACAACCAACAGAACACCGTGACGGCCCAAGGCCGTGCCGAGAAGCGCGAGGGCTCGGGACTTGTGATCCACAATTGCAGAATCGTGCCGGAACAGAAGCTGTTCGCGGAGAGGTTCAAGATCCCGACATTCTTGGGCCGGCCATGGAAGCAATACGCATTGACGGTGTTCATGGAGTCGACATTGGGGGATCTGGTTCAGCCAGCAGGGTACATGCCGTGGTCTGGGAACTTCGCTCTGGACACTTGCCAGTACTTGGAGTATGCGAACAAAGGACCTGGAGCGAACACGAACAGGAGAGTGAGGTGGAAGGGAGTGAGAGTGATGGGGAGAAACGAGGCGGTGAAGTACACAGCTGGGCCATTCCTTCTGGGAAACCAATGGCTGAAGGGCACTGGTGGACAGTACTTCTTGGGATTGAAGAACTGAAGAAATGGCGTTCTTGGGTTGGTGAAGAAGAAGAAAGAGATTTCATTTCAATTCATTTCATTTCAGGGCATTGGTTTGAGGCTATCCGTTCTTTGTAATGTGGATTGGGAT

mRNA sequence

AAAATTGTGGTCTGTTTGAGAATGATCTTCCCAAATGAGTAGAAAAAGAACACTAAAATTAAACCACAAATCCCTCAAATTTATACCTGCATTCCTAAAAAACAGCTCCTATTTTTGGCAAACTTCACCACTATATAATCTTTCCCATGCAGACCATTTCTCATCACCTCCTCCATTCAATATCATTACAAAAATCCCAAAAAAAAATAAAAACAATACTCAAAACCCACTCTTTTTCCCCATCTTTCTGGTGGGAAAAGCTTCTCCCACCTGGGTTTTGATCATTCATTAACAGGAGGAAGGCCTTTTCCACATTACAAGAAACAGGAGGGAGCAAAAACACATAAAATAAAATGGTAGGGAAGGTGGTCGTGTCGGGAATATCCCTTATTCTCGTCGTCGGTGTAGCTCTCGCCGTCGTCGCCGTCGTTAACAAGAACAATAGTTCCGAAGCTGCGACGGAGGATCTGTCTCCAAAGATGAAGGCAGTCTCAACCATTTGTTCTGGTGCTGATTACCGTAAAGAATGCCAAACTACTCTTTCCAGCGTTGCTCAAAATACCTCTTCAGATGACCCCAAGGAGTATCTTAAAGCGGCAGTTCTTGCCACTATAGATGAGATTAAGAAAGGCTACAACTTGTCCGATAGCTTTCTAGTCGAGCCTATTAACAATGCCACCATCAAAATGTCCATTGAGGATTGCAGGGACTTACTGCAGTTCGCCATCGACGAACTGCAGGCGTCTGCCTCCGCTGTCGGGGACCCCACAATGCACTCTGAAGCAGACCGCGCTGCCGATATCAGGAATTGGCTCAGCGCCGTCGTTTCTTATCAGCAATCTTGCCTCGACGGACTCGAAGAAACCGACCCCCAGCTCAAACAGAAGATGCAAGACGGCCTAGATGGTGCTGGCAAGCTTACTAGCAATGCTCTTGCCGTTCTCGACGCAGTTACCGAAATTCTCGAAAGCCTGGGCTTGCAGTTGAAGGTCCAACCAACTGGCCGTAGGTTGCTTGGAACAACAGAAGTAGGGAGCGACGGGTTCCCGACGTGGTTATCGGCGGCAGATAGAAAGCTGCTGGCAGCCAGAGGAGGAGGAGCCCCCCGAGTGAGGCCGAACGCAGTGGTGGCGAAGGACGGTAGCGGGCAGTTCAAAACCATTGCTGCTGCATTGGCTGCATATCCAAAGGTTCCAAGAGGAAGATATATAATCTACGTGAAAGCAGGGATCTATGATGAGTACATCACTGTGGATCAAAACATGAAGAATGTTTTCATGTATGGTGATGGCCCAAGAAAGACCATTGTCACTGGCCGCAAGTGCAATCGTGATGGCATCACCACCCAAAACACTGCCTCTTTCTCTGCCATAGGAGACGATTTCCTGTGCAAATCGATGGGATTCCAAAACACAGCCGGCCCCCAAGGGCACCAAGCCGTAGCCCTAAGAGTCCAATCCGACAGGTCGGCGTTCTTCAACTGCAGAATGGACGGCTACCAAGACACCCTGTACGTGCAAACCCATCGTCAATTCTACCGCAACTGCGTGATCTCCGGCACCGTGGACTTCATCTTCGGCGATTCAGCGACGATCATCCAGAACTCGTTGATCATCGTCCGACGCCCAATGGACAACCAACAGAACACCGTGACGGCCCAAGGCCGTGCCGAGAAGCGCGAGGGCTCGGGACTTGTGATCCACAATTGCAGAATCGTGCCGGAACAGAAGCTGTTCGCGGAGAGGTTCAAGATCCCGACATTCTTGGGCCGGCCATGGAAGCAATACGCATTGACGGTGTTCATGGAGTCGACATTGGGGGATCTGGTTCAGCCAGCAGGGTACATGCCGTGGTCTGGGAACTTCGCTCTGGACACTTGCCAGTACTTGGAGTATGCGAACAAAGGACCTGGAGCGAACACGAACAGGAGAGTGAGGTGGAAGGGAGTGAGAGTGATGGGGAGAAACGAGGCGGTGAAGTACACAGCTGGGCCATTCCTTCTGGGAAACCAATGGCTGAAGGGCACTGGTGGACAGTACTTCTTGGGATTGAAGAACTGAAGAAATGGCGTTCTTGGGTTGGTGAAGAAGAAGAAAGAGATTTCATTTCAATTCATTTCATTTCAGGGCATTGGTTTGAGGCTATCCGTTCTTTGTAATGTGGATTGGGAT

Coding sequence (CDS)

ATGGTAGGGAAGGTGGTCGTGTCGGGAATATCCCTTATTCTCGTCGTCGGTGTAGCTCTCGCCGTCGTCGCCGTCGTTAACAAGAACAATAGTTCCGAAGCTGCGACGGAGGATCTGTCTCCAAAGATGAAGGCAGTCTCAACCATTTGTTCTGGTGCTGATTACCGTAAAGAATGCCAAACTACTCTTTCCAGCGTTGCTCAAAATACCTCTTCAGATGACCCCAAGGAGTATCTTAAAGCGGCAGTTCTTGCCACTATAGATGAGATTAAGAAAGGCTACAACTTGTCCGATAGCTTTCTAGTCGAGCCTATTAACAATGCCACCATCAAAATGTCCATTGAGGATTGCAGGGACTTACTGCAGTTCGCCATCGACGAACTGCAGGCGTCTGCCTCCGCTGTCGGGGACCCCACAATGCACTCTGAAGCAGACCGCGCTGCCGATATCAGGAATTGGCTCAGCGCCGTCGTTTCTTATCAGCAATCTTGCCTCGACGGACTCGAAGAAACCGACCCCCAGCTCAAACAGAAGATGCAAGACGGCCTAGATGGTGCTGGCAAGCTTACTAGCAATGCTCTTGCCGTTCTCGACGCAGTTACCGAAATTCTCGAAAGCCTGGGCTTGCAGTTGAAGGTCCAACCAACTGGCCGTAGGTTGCTTGGAACAACAGAAGTAGGGAGCGACGGGTTCCCGACGTGGTTATCGGCGGCAGATAGAAAGCTGCTGGCAGCCAGAGGAGGAGGAGCCCCCCGAGTGAGGCCGAACGCAGTGGTGGCGAAGGACGGTAGCGGGCAGTTCAAAACCATTGCTGCTGCATTGGCTGCATATCCAAAGGTTCCAAGAGGAAGATATATAATCTACGTGAAAGCAGGGATCTATGATGAGTACATCACTGTGGATCAAAACATGAAGAATGTTTTCATGTATGGTGATGGCCCAAGAAAGACCATTGTCACTGGCCGCAAGTGCAATCGTGATGGCATCACCACCCAAAACACTGCCTCTTTCTCTGCCATAGGAGACGATTTCCTGTGCAAATCGATGGGATTCCAAAACACAGCCGGCCCCCAAGGGCACCAAGCCGTAGCCCTAAGAGTCCAATCCGACAGGTCGGCGTTCTTCAACTGCAGAATGGACGGCTACCAAGACACCCTGTACGTGCAAACCCATCGTCAATTCTACCGCAACTGCGTGATCTCCGGCACCGTGGACTTCATCTTCGGCGATTCAGCGACGATCATCCAGAACTCGTTGATCATCGTCCGACGCCCAATGGACAACCAACAGAACACCGTGACGGCCCAAGGCCGTGCCGAGAAGCGCGAGGGCTCGGGACTTGTGATCCACAATTGCAGAATCGTGCCGGAACAGAAGCTGTTCGCGGAGAGGTTCAAGATCCCGACATTCTTGGGCCGGCCATGGAAGCAATACGCATTGACGGTGTTCATGGAGTCGACATTGGGGGATCTGGTTCAGCCAGCAGGGTACATGCCGTGGTCTGGGAACTTCGCTCTGGACACTTGCCAGTACTTGGAGTATGCGAACAAAGGACCTGGAGCGAACACGAACAGGAGAGTGAGGTGGAAGGGAGTGAGAGTGATGGGGAGAAACGAGGCGGTGAAGTACACAGCTGGGCCATTCCTTCTGGGAAACCAATGGCTGAAGGGCACTGGTGGACAGTACTTCTTGGGATTGAAGAACTGA

Protein sequence

MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQTTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDLLQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQDGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADRKLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQYFLGLKN
Homology
BLAST of Lcy09g008240 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 6.8e-123
Identity = 228/563 (40.50%), Postives = 344/563 (61.10%), Query Frame = 0

Query: 4   KVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQTTL 63
           + V+  IS +L++ + +AV   V+ N S  A  E+++  +KA+  +C+  DY++ C+ TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDLLQF 123
              A++TS  DP E +K A  AT+ +I      S + ++E   +   KM+++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQDGL 183
           AI EL  S   +G    H   +    +R WLSA +S++Q+CLDG + T     + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADRKLL 243
             A +LT N LA+   VTE+   LG     +   RRLL      S  FP+W+ A  R+LL
Sbjct: 195 KTAVQLTHNGLAM---VTEMSNYLGQMQIPEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 AARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQN 303
            A       V+P+ VVA+DGSGQ+KTI  AL   PK     +++++K GIY EY+ V+++
Sbjct: 255 NA---PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAV 363
           M ++   GDGP KT+++G K  +DGITT  TA+ + +GD F+ K++ F+NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVR 423
           A+RV +D S F+NC+ DGYQDTLY  +HRQFYR+C ISGT+DF+FGD+A + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVF 483
           +P+ NQ   +TA GR + RE +G V+  C IV E    A + +  T+LGRPWK+Y+ T+ 
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKY 543
           M + + D V P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E +K+
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGNQWLKGTGGQYFLGL 567
           T   ++ G+ W+ G G  Y LGL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Lcy09g008240 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 3.7e-121
Identity = 234/525 (44.57%), Postives = 334/525 (63.62%), Query Frame = 0

Query: 44  KAVSTICSGADYRKECQTTLSS-VAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLV 103
           K + T+CS   Y + C+ TL +   +  + D+P  +LK+A+ A  +++     + +  L 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 104 EPINNATIKMSIEDCRDLLQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQ 163
               N   K +IE C+ L++ A +E  AS + +    ++S      D+ +WLSAV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLEETDPQLKQKMQDGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLG 223
           +CLDG EE +  LK +++  ++ +  LTSN+LA++   TE L  +     ++   R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV-----MKVVERHLL- 286

Query: 224 TTEVGSDGFPTWLSAADRKLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPR 283
                 D  P+W+S  DR++L  R      ++PNA VAKDGSG F TI  AL A P+   
Sbjct: 287 ------DDIPSWVSNDDRRML--RAVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYE 346

Query: 284 GRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGD 343
           GRYIIYVK GIYDEY+TVD+   N+ M GDG +KTIVTG K +   I T  TA+F A G+
Sbjct: 347 GRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGE 406

Query: 344 DFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISG 403
            F+ +SMGF+NTAGP+GHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+YR+CVI G
Sbjct: 407 GFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVG 466

Query: 404 TVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFA 463
           T+DFIFGD+A I QN  I +R+ +  Q+NTVTAQGR +K + +G V+HNC+I   + L  
Sbjct: 467 TIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKP 526

Query: 464 ERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPW-SGNFALDTCQYLEYANKGPGA 523
            + +  ++LGRPWK Y+ T+ MES + +++ P G++ W   +FA+DT  Y EY NKG   
Sbjct: 527 VKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSG 586

Query: 524 NTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQYFLGL 567
           +T  RV+W G +V+ + EA+ YT GPFL G+ W+  +G    LGL
Sbjct: 587 DTTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of Lcy09g008240 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 4.9e-121
Identity = 252/595 (42.35%), Postives = 358/595 (60.17%), Query Frame = 0

Query: 4   KVVVSGISLILVV-----GVALAVVAVVNK---------NNSSEAATEDLSPKM------ 63
           K ++ G+  +LVV     G A A V   NK         N S ++ T+  SP        
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSTICSGADYRKECQTTL-SSVAQNTSSDDPKEYLKAAVLATIDEIKK 123
                     K + T+C+   Y+  CQ TL +   ++T   DP+  LK+A++A  D++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLSDSFLVEPINNATIKMSIEDCRDLLQFAIDELQASASAVGDPTMHSEADRAADIRN 183
            +    S   E   N   K +I  C+ L+  A +EL  S   + D  +++ A    D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVVSYQQSCLDGLEETDPQLKQKMQDGLDGAGKLTSNALAVLDAVTEILESLGLQLK 243
           WLSAV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L S+    K
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---K 262

Query: 244 VQPTGRRLLGTTEVGSDGFPTWLSAADRKLLAARGGGAPRVRPNAVVAKDGSGQFKTIAA 303
           V+        ++   +D   +WLS  +R++L A    A  ++PNA VAKDGSG F TI A
Sbjct: 263 VKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKA--LKPNATVAKDGSGNFTTINA 322

Query: 304 ALAAYPKVPRGRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQ 363
           AL A P   +GRY IY+K GIYDE + +D+   NV M GDG +KTIVTG K +   I T 
Sbjct: 323 ALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTF 382

Query: 364 NTASFSAIGDDFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHR 423
            TA+F A G+ F+ +SMGF+NTAGP+GHQAVA+RVQSDRS F NCR +GYQDTLY  THR
Sbjct: 383 LTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHR 442

Query: 424 QFYRNCVISGTVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREGSGLVIHNC 483
           Q+YR+CVI GTVDFIFGD+A I QN  I +R+ +  Q+NTVTAQGR +K + +G VIHNC
Sbjct: 443 QYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNC 502

Query: 484 RIVPEQKLFAERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPW-SGNFALDTCQY 543
            + P + L   + +  ++LGRPWK ++ TV MEST+ D++ P G++ W   +FA+DT  Y
Sbjct: 503 TVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSY 562

Query: 544 LEYANKGPGANTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQYFLGL 567
            EY N GP   T  RV+W G RV+ + EA+K+T GPFL G +W++  G    LGL
Sbjct: 563 AEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG-EWIQAIGSPVKLGL 606

BLAST of Lcy09g008240 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 429.9 bits (1104), Expect = 4.6e-119
Identity = 222/562 (39.50%), Postives = 341/562 (60.68%), Query Frame = 0

Query: 5   VVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQTTLS 64
           + +S + LI +V VA+ V   +NK++       +++  +KAV  +C+  DYRK C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  SVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDLLQFA 124
              +NT+  DP E +K A   T+ +I      S + ++E   ++  +M+++ C++L+ +A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQDGLD 184
           +DEL  S   +G    H   +   ++R WLSA +S++++CL+G + T     + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 GAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADRKLLA 244
            A +LT N LA+   ++E+   +G         RRLL      ++GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAI---ISEMSNFVGQMQIPGLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 ARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQNM 304
           A    +  V+P+ VVA+DGSGQ+KTI  AL   PK     +++++KAG+Y EY+ V++ M
Sbjct: 258 AAAAYSD-VKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTM 317

Query: 305 KNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAVA 364
            ++   GDGP KTI++G K  +DGITT  TA+ + +G+ F+ K++GF+NTAG   HQAVA
Sbjct: 318 SHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVA 377

Query: 365 LRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVRR 424
           +RVQSD S FFNCR DGYQDTLY  +HRQF+R+C ISGT+DF+FGD+A + QN  ++VR+
Sbjct: 378 VRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRK 437

Query: 425 PMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVFM 484
           P+ NQ   +TA GR + RE +G V   C I  E    A +     +LGRPWK+Y+ T+ M
Sbjct: 438 PLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIM 497

Query: 485 ESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKYT 544
            + + D VQP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + +K+T
Sbjct: 498 NTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFT 557

Query: 545 AGPFLLGNQWLKGTGGQYFLGL 567
              ++ G+ W+ G G  Y  GL
Sbjct: 558 PAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Lcy09g008240 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.3e-118
Identity = 250/604 (41.39%), Postives = 351/604 (58.11%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           M+GKVVVS  S++L+VGVA+ VVA +NKN  +     +LSP+MKAV  IC     +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNAT--IKMSIEDCR 120
            TL  V     S+DP + +KA +LAT DE+ K  N +    V   ++ +   K  ++ C+
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQK 180
            +  +A+++L      +G+  +     +   ++ WL  V +YQ  CLD +EE D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDGAGKLTSNALAVLDAVTEILESLGLQLK-----------------------VQP 240
           + +G+  +  LT+NA+ +   V   +  +  ++                          P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 TG--RRLLGTTEVGSDGFPTWLSAADRKLLAARG-------GGAPRVRPNAVVAKDGSGQ 300
            G  RRLL   ++   G PTW+S ADRKL+A  G       GG  RVR N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRK--C 360
           FKT+  A+ A P+  RGR IIY+KAG+Y E + + +   N+FM+GDG RKT+++  +   
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQD 420
              G TT  +A+     + F+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DGYQD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREG 480
           TLYV   RQFYRNCV+SGTVDFIFG SAT+IQN+LI+VR+    Q NTVTA G  E   G
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGN-ELGLG 480

Query: 481 S--GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPWSG 540
              G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TV M + +GDL++P G+  W G
Sbjct: 481 MKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDG 540

Query: 541 NFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQY 567
                +C+Y+EY N+GPGA  NRRV W  V      E   +TA  +L    W++      
Sbjct: 541 ESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPV 588

BLAST of Lcy09g008240 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.0e-266
Identity = 461/568 (81.16%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MVGKV+VS +SLILVVG+ALAVVA +NK N S    E++SPKMKA+S ICS  DY++EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TL+ VAQNTSS+DPK+Y KAA+LATI EI KGYNLSDS +VE  NNA+IKMS+EDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAIDELQAS S VG+  +H++ADR ADI+NWLSAVVSYQQSCLDGL E DPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGLD AGKLTSNALA+L AV+ IL++ GLQLKVQP+GRRLLGTTEV SDGFPTWL+ ADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA++ GG  R  PNAVVAKDGSGQ+KTIAAALAAYPK  RGRY+IYVKAGIYDEYITV
Sbjct: 241 KLLASKQGGV-RPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKNVFMYGDGPRKTIVTGRK NRDG TTQNTASF+AIG+ FLCKSMGF NTAGPQGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTAQGRAE++E +GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QPAGYMPWSGNFAL+TC YLEY N+GPGANTNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           ++YTAG FLLG  WL  TGG Y+LGLKN
Sbjct: 541 LQYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lcy09g008240 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 9.8e-266
Identity = 459/568 (80.81%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MVGKV+VS +SLILVVG+ALAVVA +NK N S    E++SPKMKA+S ICS  DY++EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TL+ VA NTSS+DPK+Y KAA+LATI EI KGYNLSDS +VE  NNA+IKMS+EDC+DL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAIDELQAS S VG+  +H++ADR ADI+NWLSAVVSYQQSCLDGL E DPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGLD AGKLTSNALA++ AV+ IL++ GLQLKVQP+GRRLLGTTEV SDGFPTWL+ ADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA++ GG  R  PNAVVAKDGSGQ+KTIAAALAAYPK  RGRY+IYVKAGIYDEYITV
Sbjct: 241 KLLASKQGGV-RPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKNVFMYGDGPRKTIVTGRK NRDG TTQNTASF+AIG+ FLCKSMGF NTAGPQGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTAQGRAE++E +GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QPAGYMPWSGNFAL+TC YLEY N+GPGANTNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           ++YTAG FLLG  WL  TGG Y+LGLKN
Sbjct: 541 LQYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lcy09g008240 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.4e-264
Identity = 448/568 (78.87%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MV KV++ GISLILVVGVALAVVA+V+ N SS+AATEDLSPKMKAVSTIC+ A+Y++ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TLS+ A N SSDDPKEY++AA+L+TI+E+KKGYNL+D F++E  NN +IKM +EDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAID+LQAS S VG+P +H+ ADR ADI+NWL++V+SYQQSCLDGLEE DPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGL  AGKLTSNALA++DAV++IL S GLQLK QP+GRRLLGTTEVG DG+PTWL+ ADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA+RGGGA +V+PNAVVAKDGSGQFKTI+AALAAYPK  RGRY+IYVKAGIY+EY+ +
Sbjct: 241 KLLASRGGGA-KVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKN+FMYGDGP+KTIVTG K NR G TTQ+TA+F AIG+ FLCKSMGFQNTAGP+GH
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTA GRA+ +E SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QP GYMPWSGNFAL+TC YLEY N+GPGA TNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           +++TAGPFL G  WL  TGG Y LGLK+
Sbjct: 541 MQFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lcy09g008240 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 5.4e-264
Identity = 447/568 (78.70%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MV KV++ GI+LILVVGVALAVVA+V+ N SS+AATEDLSPKMKAVSTIC+ A+Y++ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TLS+ A N SSDDPKEY++AA+L+TI+E+KKGYNL+D F++E  NN +IKM +EDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAID+LQAS S VG+P +H+ ADR ADI+NWL++V+SYQQSCLDGLEE DPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGL  AGKLTSNALA++DAV++IL S GLQLK QP+GRRLLGTTEVG DG+PTWL+ ADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA+RGGGA +V+PNAVVAKDGSGQFKTI+AALAAYPK  RGRY+IYVKAGIY+EY+ +
Sbjct: 241 KLLASRGGGA-KVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKN+FMYGDGP+KTIVTG K NR G TTQ+TA+F AIG+ FLCKSMGFQNTAGP+GH
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTA GRA+ +E SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QP GYMPWSGNFAL+TC YLEY N+GPGA TNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           +++TAGPFL G  WL  TGG Y LGLK+
Sbjct: 541 MQFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lcy09g008240 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.2e-263
Identity = 454/568 (79.93%), Postives = 512/568 (90.14%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MVGKVV+SGISLILVVGVALAVVA+VNK++SS+  T +LSPKMKAV++ICS ADY+ ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
           TTL SV  N+S +DPKE++KAA+ +TI+E+KKGYNL+D+ +VE  NNATIKMS++DC+DL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQ AIDEL AS S VGDP +H+  DR ADI+NWL+AV+SYQQSCLDGLEE DPQLKQKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGLD AGKLTSNALA++ AV++IL+ LGLQLKVQP+GRRLLGTTEV SD FPTWL+ +DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLAA+ GG  RV+PN VVAKDGSGQ+KTI AALAAYPK  +GRY+IYVKAG+Y+E I V
Sbjct: 241 KLLAAKRGGV-RVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIV 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKN+FMYGDGPRKTIVTGRK NRDGITTQNTASF+AIG+ FLCKSMGF NTAGP+GH
Sbjct: 301 TKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDS TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTA GRA+K+E SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YA 
Sbjct: 421 IVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QPAGYMPWSGNFAL TC Y EY N+GPGANTNRRVRWKGVRV+GRNEA
Sbjct: 481 TVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           +++TAGPFLLG  WL GTGG Y LGLKN
Sbjct: 541 MQFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Lcy09g008240 vs. NCBI nr
Match: XP_008466403.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 927.9 bits (2397), Expect = 4.1e-266
Identity = 461/568 (81.16%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MVGKV+VS +SLILVVG+ALAVVA +NK N S    E++SPKMKA+S ICS  DY++EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TL+ VAQNTSS+DPK+Y KAA+LATI EI KGYNLSDS +VE  NNA+IKMS+EDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAIDELQAS S VG+  +H++ADR ADI+NWLSAVVSYQQSCLDGL E DPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGLD AGKLTSNALA+L AV+ IL++ GLQLKVQP+GRRLLGTTEV SDGFPTWL+ ADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA++ GG  R  PNAVVAKDGSGQ+KTIAAALAAYPK  RGRY+IYVKAGIYDEYITV
Sbjct: 241 KLLASKQGGV-RPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKNVFMYGDGPRKTIVTGRK NRDG TTQNTASF+AIG+ FLCKSMGF NTAGPQGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTAQGRAE++E +GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QPAGYMPWSGNFAL+TC YLEY N+GPGANTNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           ++YTAG FLLG  WL  TGG Y+LGLKN
Sbjct: 541 LQYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lcy09g008240 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 927.5 bits (2396), Expect = 5.3e-266
Identity = 463/569 (81.37%), Postives = 514/569 (90.33%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDL-SPKMKAVSTICSGADYRKEC 60
           MVGKVVVSGISLILVVGVALAVVA+V KNN+S+    DL SPKMKAVSTICS  DY++EC
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDV---DLSSPKMKAVSTICSTTDYQQEC 60

Query: 61  QTTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRD 120
           Q TL++VAQN S DDPKEY KAAVLATI EI KGYNL+   +VE  NNA+IKMS++DC+D
Sbjct: 61  QNTLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKD 120

Query: 121 LLQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKM 180
           LLQ AIDEL AS S VGDP +H+  DR ADI+NWL+AV+SYQQSCLDGLEE DPQLKQKM
Sbjct: 121 LLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180

Query: 181 QDGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAAD 240
           QDGLD AGKLTSNALA++ AV++IL++ GLQLKVQP+GRRLL TTE+GSDGFPTW+S AD
Sbjct: 181 QDGLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGAD 240

Query: 241 RKLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYIT 300
           RKLLA+RGGGA RV+PNAVVAKDGSGQ+K+I AALAAYPK  RGRY+IYVKAGIYDEYIT
Sbjct: 241 RKLLASRGGGA-RVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYIT 300

Query: 301 VDQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQG 360
           V ++MKNVFMYGDGPRKTIVTGRK NRDGITTQNTASFSAIG+ FLCKSMGF+NTAGP+G
Sbjct: 301 VTKDMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEG 360

Query: 361 HQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSL 420
           HQAVALRVQSDRSAF+NCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSL
Sbjct: 361 HQAVALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSL 420

Query: 421 IIVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 480
           IIVRRPMDNQ+NTVTA GRA+K+E SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA
Sbjct: 421 IIVRRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 480

Query: 481 LTVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNE 540
            TV ME+TLGD +QP GYMPWSGNFAL+TC Y EY N+GPGANTNRRVRWKGVRV+GRNE
Sbjct: 481 RTVIMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNE 540

Query: 541 AVKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           A++YTAGPFL+G  WL  TGG Y LGLK+
Sbjct: 541 ALQYTAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of Lcy09g008240 vs. NCBI nr
Match: KAA0050274.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 925.6 bits (2391), Expect = 2.0e-265
Identity = 459/568 (80.81%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MVGKV+VS +SLILVVG+ALAVVA +NK N S    E++SPKMKA+S ICS  DY++EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TL+ VA NTSS+DPK+Y KAA+LATI EI KGYNLSDS +VE  NNA+IKMS+EDC+DL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAIDELQAS S VG+  +H++ADR ADI+NWLSAVVSYQQSCLDGL E DPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGLD AGKLTSNALA++ AV+ IL++ GLQLKVQP+GRRLLGTTEV SDGFPTWL+ ADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA++ GG  R  PNAVVAKDGSGQ+KTIAAALAAYPK  RGRY+IYVKAGIYDEYITV
Sbjct: 241 KLLASKQGGV-RPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKNVFMYGDGPRKTIVTGRK NRDG TTQNTASF+AIG+ FLCKSMGF NTAGPQGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTAQGRAE++E +GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QPAGYMPWSGNFAL+TC YLEY N+GPGANTNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           ++YTAG FLLG  WL  TGG Y+LGLKN
Sbjct: 541 LQYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lcy09g008240 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 921.0 bits (2379), Expect = 5.0e-264
Identity = 448/568 (78.87%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MV KV++ GISLILVVGVALAVVA+V+ N SS+AATEDLSPKMKAVSTIC+ A+Y++ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TLS+ A N SSDDPKEY++AA+L+TI+E+KKGYNL+D F++E  NN +IKM +EDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAID+LQAS S VG+P +H+ ADR ADI+NWL++V+SYQQSCLDGLEE DPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGL  AGKLTSNALA++DAV++IL S GLQLK QP+GRRLLGTTEVG DG+PTWL+ ADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA+RGGGA +V+PNAVVAKDGSGQFKTI+AALAAYPK  RGRY+IYVKAGIY+EY+ +
Sbjct: 241 KLLASRGGGA-KVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKN+FMYGDGP+KTIVTG K NR G TTQ+TA+F AIG+ FLCKSMGFQNTAGP+GH
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTA GRA+ +E SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QP GYMPWSGNFAL+TC YLEY N+GPGA TNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           +++TAGPFL G  WL  TGG Y LGLK+
Sbjct: 541 MQFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lcy09g008240 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 919.8 bits (2376), Expect = 1.1e-263
Identity = 447/568 (78.70%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           MV KV++ GI+LILVVGVALAVVA+V+ N SS+AATEDLSPKMKAVSTIC+ A+Y++ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDL 120
            TLS+ A N SSDDPKEY++AA+L+TI+E+KKGYNL+D F++E  NN +IKM +EDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQ 180
           LQFAID+LQAS S VG+P +H+ ADR ADI+NWL++V+SYQQSCLDGLEE DPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADR 240
           DGL  AGKLTSNALA++DAV++IL S GLQLK QP+GRRLLGTTEVG DG+PTWL+ ADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITV 300
           KLLA+RGGGA +V+PNAVVAKDGSGQFKTI+AALAAYPK  RGRY+IYVKAGIY+EY+ +
Sbjct: 241 KLLASRGGGA-KVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 DQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGH 360
            ++MKN+FMYGDGP+KTIVTG K NR G TTQ+TA+F AIG+ FLCKSMGFQNTAGP+GH
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAL 480
           IVRRPMDNQQNTVTA GRA+ +E SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 
Sbjct: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVFMESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEA 540
           TV MESTLGD +QP GYMPWSGNFAL+TC YLEY N+GPGA TNRRVRWKGV+V+GRNEA
Sbjct: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540

Query: 541 VKYTAGPFLLGNQWLKGTGGQYFLGLKN 569
           +++TAGPFL G  WL  TGG Y LGLK+
Sbjct: 541 MQFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lcy09g008240 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 442.6 bits (1137), Expect = 4.8e-124
Identity = 228/563 (40.50%), Postives = 344/563 (61.10%), Query Frame = 0

Query: 4   KVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQTTL 63
           + V+  IS +L++ + +AV   V+ N S  A  E+++  +KA+  +C+  DY++ C+ TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDLLQF 123
              A++TS  DP E +K A  AT+ +I      S + ++E   +   KM+++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQDGL 183
           AI EL  S   +G    H   +    +R WLSA +S++Q+CLDG + T     + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADRKLL 243
             A +LT N LA+   VTE+   LG     +   RRLL      S  FP+W+ A  R+LL
Sbjct: 195 KTAVQLTHNGLAM---VTEMSNYLGQMQIPEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 AARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQN 303
            A       V+P+ VVA+DGSGQ+KTI  AL   PK     +++++K GIY EY+ V+++
Sbjct: 255 NA---PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAV 363
           M ++   GDGP KT+++G K  +DGITT  TA+ + +GD F+ K++ F+NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVR 423
           A+RV +D S F+NC+ DGYQDTLY  +HRQFYR+C ISGT+DF+FGD+A + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVF 483
           +P+ NQ   +TA GR + RE +G V+  C IV E    A + +  T+LGRPWK+Y+ T+ 
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKY 543
           M + + D V P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E +K+
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGNQWLKGTGGQYFLGL 567
           T   ++ G+ W+ G G  Y LGL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Lcy09g008240 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 436.8 bits (1122), Expect = 2.7e-122
Identity = 234/525 (44.57%), Postives = 334/525 (63.62%), Query Frame = 0

Query: 44  KAVSTICSGADYRKECQTTLSS-VAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLV 103
           K + T+CS   Y + C+ TL +   +  + D+P  +LK+A+ A  +++     + +  L 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 104 EPINNATIKMSIEDCRDLLQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQ 163
               N   K +IE C+ L++ A +E  AS + +    ++S      D+ +WLSAV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLEETDPQLKQKMQDGLDGAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLG 223
           +CLDG EE +  LK +++  ++ +  LTSN+LA++   TE L  +     ++   R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV-----MKVVERHLL- 286

Query: 224 TTEVGSDGFPTWLSAADRKLLAARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPR 283
                 D  P+W+S  DR++L  R      ++PNA VAKDGSG F TI  AL A P+   
Sbjct: 287 ------DDIPSWVSNDDRRML--RAVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYE 346

Query: 284 GRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGD 343
           GRYIIYVK GIYDEY+TVD+   N+ M GDG +KTIVTG K +   I T  TA+F A G+
Sbjct: 347 GRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGE 406

Query: 344 DFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISG 403
            F+ +SMGF+NTAGP+GHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+YR+CVI G
Sbjct: 407 GFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVG 466

Query: 404 TVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFA 463
           T+DFIFGD+A I QN  I +R+ +  Q+NTVTAQGR +K + +G V+HNC+I   + L  
Sbjct: 467 TIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKP 526

Query: 464 ERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPW-SGNFALDTCQYLEYANKGPGA 523
            + +  ++LGRPWK Y+ T+ MES + +++ P G++ W   +FA+DT  Y EY NKG   
Sbjct: 527 VKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSG 586

Query: 524 NTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQYFLGL 567
           +T  RV+W G +V+ + EA+ YT GPFL G+ W+  +G    LGL
Sbjct: 587 DTTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of Lcy09g008240 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 436.4 bits (1121), Expect = 3.5e-122
Identity = 252/595 (42.35%), Postives = 358/595 (60.17%), Query Frame = 0

Query: 4   KVVVSGISLILVV-----GVALAVVAVVNK---------NNSSEAATEDLSPKM------ 63
           K ++ G+  +LVV     G A A V   NK         N S ++ T+  SP        
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSTICSGADYRKECQTTL-SSVAQNTSSDDPKEYLKAAVLATIDEIKK 123
                     K + T+C+   Y+  CQ TL +   ++T   DP+  LK+A++A  D++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLSDSFLVEPINNATIKMSIEDCRDLLQFAIDELQASASAVGDPTMHSEADRAADIRN 183
            +    S   E   N   K +I  C+ L+  A +EL  S   + D  +++ A    D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVVSYQQSCLDGLEETDPQLKQKMQDGLDGAGKLTSNALAVLDAVTEILESLGLQLK 243
           WLSAV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L S+    K
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---K 262

Query: 244 VQPTGRRLLGTTEVGSDGFPTWLSAADRKLLAARGGGAPRVRPNAVVAKDGSGQFKTIAA 303
           V+        ++   +D   +WLS  +R++L A    A  ++PNA VAKDGSG F TI A
Sbjct: 263 VKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKA--LKPNATVAKDGSGNFTTINA 322

Query: 304 ALAAYPKVPRGRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRKCNRDGITTQ 363
           AL A P   +GRY IY+K GIYDE + +D+   NV M GDG +KTIVTG K +   I T 
Sbjct: 323 ALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTF 382

Query: 364 NTASFSAIGDDFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHR 423
            TA+F A G+ F+ +SMGF+NTAGP+GHQAVA+RVQSDRS F NCR +GYQDTLY  THR
Sbjct: 383 LTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHR 442

Query: 424 QFYRNCVISGTVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREGSGLVIHNC 483
           Q+YR+CVI GTVDFIFGD+A I QN  I +R+ +  Q+NTVTAQGR +K + +G VIHNC
Sbjct: 443 QYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNC 502

Query: 484 RIVPEQKLFAERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPW-SGNFALDTCQY 543
            + P + L   + +  ++LGRPWK ++ TV MEST+ D++ P G++ W   +FA+DT  Y
Sbjct: 503 TVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSY 562

Query: 544 LEYANKGPGANTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQYFLGL 567
            EY N GP   T  RV+W G RV+ + EA+K+T GPFL G +W++  G    LGL
Sbjct: 563 AEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG-EWIQAIGSPVKLGL 606

BLAST of Lcy09g008240 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 429.9 bits (1104), Expect = 3.2e-120
Identity = 222/562 (39.50%), Postives = 341/562 (60.68%), Query Frame = 0

Query: 5   VVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQTTLS 64
           + +S + LI +V VA+ V   +NK++       +++  +KAV  +C+  DYRK C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  SVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNATIKMSIEDCRDLLQFA 124
              +NT+  DP E +K A   T+ +I      S + ++E   ++  +M+++ C++L+ +A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQKMQDGLD 184
           +DEL  S   +G    H   +   ++R WLSA +S++++CL+G + T     + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 GAGKLTSNALAVLDAVTEILESLGLQLKVQPTGRRLLGTTEVGSDGFPTWLSAADRKLLA 244
            A +LT N LA+   ++E+   +G         RRLL      ++GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAI---ISEMSNFVGQMQIPGLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 ARGGGAPRVRPNAVVAKDGSGQFKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQNM 304
           A    +  V+P+ VVA+DGSGQ+KTI  AL   PK     +++++KAG+Y EY+ V++ M
Sbjct: 258 AAAAYSD-VKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTM 317

Query: 305 KNVFMYGDGPRKTIVTGRKCNRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAVA 364
            ++   GDGP KTI++G K  +DGITT  TA+ + +G+ F+ K++GF+NTAG   HQAVA
Sbjct: 318 SHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVA 377

Query: 365 LRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVRR 424
           +RVQSD S FFNCR DGYQDTLY  +HRQF+R+C ISGT+DF+FGD+A + QN  ++VR+
Sbjct: 378 VRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRK 437

Query: 425 PMDNQQNTVTAQGRAEKREGSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVFM 484
           P+ NQ   +TA GR + RE +G V   C I  E    A +     +LGRPWK+Y+ T+ M
Sbjct: 438 PLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIM 497

Query: 485 ESTLGDLVQPAGYMPWSGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKYT 544
            + + D VQP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + +K+T
Sbjct: 498 NTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFT 557

Query: 545 AGPFLLGNQWLKGTGGQYFLGL 567
              ++ G+ W+ G G  Y  GL
Sbjct: 558 PAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Lcy09g008240 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 428.3 bits (1100), Expect = 9.5e-120
Identity = 250/604 (41.39%), Postives = 351/604 (58.11%), Query Frame = 0

Query: 1   MVGKVVVSGISLILVVGVALAVVAVVNKNNSSEAATEDLSPKMKAVSTICSGADYRKECQ 60
           M+GKVVVS  S++L+VGVA+ VVA +NKN  +     +LSP+MKAV  IC     +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  TTLSSVAQNTSSDDPKEYLKAAVLATIDEIKKGYNLSDSFLVEPINNAT--IKMSIEDCR 120
            TL  V     S+DP + +KA +LAT DE+ K  N +    V   ++ +   K  ++ C+
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASASAVGDPTMHSEADRAADIRNWLSAVVSYQQSCLDGLEETDPQLKQK 180
            +  +A+++L      +G+  +     +   ++ WL  V +YQ  CLD +EE D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDGAGKLTSNALAVLDAVTEILESLGLQLK-----------------------VQP 240
           + +G+  +  LT+NA+ +   V   +  +  ++                          P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 TG--RRLLGTTEVGSDGFPTWLSAADRKLLAARG-------GGAPRVRPNAVVAKDGSGQ 300
            G  RRLL   ++   G PTW+S ADRKL+A  G       GG  RVR N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTIAAALAAYPKVPRGRYIIYVKAGIYDEYITVDQNMKNVFMYGDGPRKTIVTGRK--C 360
           FKT+  A+ A P+  RGR IIY+KAG+Y E + + +   N+FM+GDG RKT+++  +   
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGITTQNTASFSAIGDDFLCKSMGFQNTAGPQGHQAVALRVQSDRSAFFNCRMDGYQD 420
              G TT  +A+     + F+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DGYQD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVISGTVDFIFGDSATIIQNSLIIVRRPMDNQQNTVTAQGRAEKREG 480
           TLYV   RQFYRNCV+SGTVDFIFG SAT+IQN+LI+VR+    Q NTVTA G  E   G
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGN-ELGLG 480

Query: 481 S--GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYALTVFMESTLGDLVQPAGYMPWSG 540
              G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TV M + +GDL++P G+  W G
Sbjct: 481 MKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDG 540

Query: 541 NFALDTCQYLEYANKGPGANTNRRVRWKGVRVMGRNEAVKYTAGPFLLGNQWLKGTGGQY 567
                +C+Y+EY N+GPGA  NRRV W  V      E   +TA  +L    W++      
Sbjct: 541 ESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPV 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E8Z86.8e-12340.50Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q7Y2013.7e-12144.57Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY64.9e-12142.35Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q8GX864.6e-11939.50Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
O807221.3e-11841.39Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CSH22.0e-26681.16Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1[more]
A0A5A7U4V79.8e-26680.81Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110... [more]
A0A5A7U9J82.4e-26478.87Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR275.4e-26478.70Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A0A0KLN21.2e-26379.93Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008466403.14.1e-26681.16PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875879.15.3e-26681.37LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
KAA0050274.12.0e-26580.81pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [... [more]
KAA0050275.15.0e-26478.87pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
XP_008466374.11.1e-26378.70PREDICTED: pectinesterase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G27870.14.8e-12440.50Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.12.7e-12244.57Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.13.5e-12242.35Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.13.2e-12039.50Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.19.5e-12041.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..196
e-value: 7.0E-33
score: 125.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 22..198
e-value: 1.1E-19
score: 68.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..195
e-value: 3.8E-24
score: 85.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 250..566
e-value: 8.6E-108
score: 362.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 43..200
e-value: 3.0E-29
score: 103.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 44..199
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 256..551
e-value: 4.0E-128
score: 426.3
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 5..558
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 5..558
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..199
e-value: 1.51805E-47
score: 161.067
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 400..409
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 281..300
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 253..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g008240.1Lcy09g008240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity