Lcy09g004560 (gene) Sponge gourd (P93075) v1

BLAST of Lcy09g004560 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 4.2e-183
Identity = 329/526 (62.55%), Postives = 397/526 (75.48%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGKGST 74
           YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLE+ + +M +IHVAGTKGKGST
Sbjct: 67  YEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGST 126

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E+
Sbjct: 127 CTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNEE 186

Query: 135 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           +PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME+
Sbjct: 187 IPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHMEI 246

Query: 195 LGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELIHGR 254
           LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   L+ G+
Sbjct: 247 LGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQ 306

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEGLTTASFEGRAQI 314
           KLGL+GEHQY+NAGLA++L+S WL + G  ++        LPE+FI+GL TAS +GRAQ+
Sbjct: 307 KLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQV 366

Query: 315 VPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQQLENNGTSSNKL 374
           VPD+YT+  +  DLVFYLDGAHSPESME C  WFS A+K + Q   +  L N    S+  
Sbjct: 367 VPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSHD 426

Query: 375 LQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKALFVPNTSVFDKV 434
               ++ E      Q+LLFNCMSVRDP LLLP L   CA  GV+FKKALFVPN SV+ KV
Sbjct: 427 KWSNETCE------QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKV 486

Query: 435 GTHG-LPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENEKPEVGGKSCEN 494
           GT   LPE + QVDLSWQF +++VW+S++Q ++                  E  G+S  N
Sbjct: 487 GTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESDGN 546

Query: 495 STVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           S VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 547 SEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of Lcy09g004560 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.7e-161
Identity = 302/526 (57.41%), Postives = 374/526 (71.10%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LED++SQMKIIHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELIHGR 254
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLA+AL S +L   G    + ++ +  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQQLENNGTSSNKL 374
           VPD     E PE++V+YLDGAHSPESME C  WFS  IK NQ+ N               
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN--------------- 372

Query: 375 LQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKALFVPNTSVFDKV 434
                    +K+S Q+LLFNCMSVRDP LLLP L   C + GV FK+A+FVPN SV+++V
Sbjct: 373 ---------QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 435 GTHGLPEANGQVD-LSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENEKPEVGGKSCEN 494
           G+        +V+ +SWQF ++R+W+S+ + +  S+SK  S             GK  E 
Sbjct: 433 GSS--TNVGTRVESMSWQFGLQRIWESLARGEAKSNSKSDSK------------GKEEEK 491

Query: 495 STVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           S VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of Lcy09g004560 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-150
Identity = 286/551 (51.91%), Postives = 380/551 (68.97%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGK 74
           Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLED + ++K+IHVAGTKGK
Sbjct: 83  YDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTKGK 142

Query: 75  GSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKT 134
           GSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKEK 
Sbjct: 143 GSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKEKA 202

Query: 135 SEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDH 194
            + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV++ P+VCGIASLG DH
Sbjct: 203 VDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGMDH 262

Query: 195 MELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELI 254
           M++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+P+ +
Sbjct: 263 MDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPKKL 322

Query: 255 HGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSR--SLPEQFIEGLTTASFE 314
            G  LGL G+HQ +NAGLA++LS  WL RTG+ K  +   S+   +P  F  GL TA   
Sbjct: 323 DGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARLH 382

Query: 315 GRAQIVPDRYTD-------IESP-EDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLN 374
           GRAQ+V D  +D       +E+P  DL+FYLDGAHSPESME CG WFS+A++    G+ +
Sbjct: 383 GRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVR----GDKS 442

Query: 375 QQLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKK 434
                NG     +  G    +  + S Q+LLFNCM VRDPQ+LLP L+ +CA++G HF +
Sbjct: 443 LSTAVNG----YMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFSR 502

Query: 435 ALFVPNTSVFDKV--GTHGLPEANGQVDLSWQFHVRRVWD-SIMQCDKGSD----SKGTS 494
           ALFVP+ S ++KV  G   +P    + DL+WQF ++R+W+ SI   D G D      G +
Sbjct: 503 ALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGIT 562

Query: 495 AVLEEE---NEKPEVGGKS---CENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSL 538
           A+   +    + P+ GG +     +S V  SLP+ I WLRD V ++N S++L+VLVTGSL
Sbjct: 563 ALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVTGSL 622

BLAST of Lcy09g004560 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-82
Identity = 212/583 (36.36%), Postives = 296/583 (50.77%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCD----KSGD---RFELMFEYVKMLDLE-DSMSQMKIIHVAGT 74
           Y++A+  L++L  N    +    K GD   + E M  Y+    L+ + + Q+ IIH+ GT
Sbjct: 42  YQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGT 101

Query: 75  KGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLK 134
           KGKGSTC FTE ILR+ G  TG F+SPHL+ +RER R++G  IS E F  +FW  Y+RL+
Sbjct: 102 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLE 161

Query: 135 EKTSED--VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIAS 194
           E   +   V MP  FRFL L+AF +F  E+VD+A+LEVG+GG +D TN++R P+VCGI S
Sbjct: 162 ETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITS 221

Query: 195 LGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSL 254
           LG DH  LLG+T+  IA +K GIFK G PAFTV QPDE + VLRD+A Q+   L + P L
Sbjct: 222 LGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPL 281

Query: 255 DPELIHG--RKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSR-SLPEQ----- 314
                 G    LGLEGEHQ  NA LA+ ++  WL + G+     ++ SR SLP Q     
Sbjct: 282 QALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAP 341

Query: 315 -------FIEGLTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAA 374
                    +GL    + GR Q++            L +YLDGAH+  SM+ C  WF  A
Sbjct: 342 VFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTWYLDGAHTTSSMQACVRWFRQA 401

Query: 375 IKHNQQGNLNQQLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKS 434
           +   ++ +   +                        V+VLLFN    RD   LL  L++ 
Sbjct: 402 LHRCERPDSGSE------------------------VRVLLFNSTGDRDSAALL-KLLQP 461

Query: 435 CANNGVHFKKALFVPNTSVFDKVGTHGLPEANGQVD--------LSWQFHVRRVWDSIMQ 494
           C      F  A+F PN +  +   T    + N  V         L+ Q H  R+ +  + 
Sbjct: 462 C-----QFDYAIFCPNLA--EMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVS 521

Query: 495 CD----KGSDSKGTSAVLEEENEKPEVGGKSCENSTVFSSLPMAIKWL---RDSVLQQ-- 538
            D     G +  G +++L      P        +S VFS +  A++W+   RD + Q   
Sbjct: 522 PDPWSTPGQEQDGPASLL----LAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPS 580

BLAST of Lcy09g004560 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 303.9 bits (777), Expect = 3.6e-81
Identity = 206/574 (35.89%), Postives = 289/574 (50.35%), Query Frame = 0

Query: 15  YEEALDALSSLIVN--------RRRCDKSGDRFELMFEYVKMLDLE-DSMSQMKIIHVAG 74
           Y++A+  L++L  N        R+R D    + E M  Y+    L+ + ++++ IIHV G
Sbjct: 45  YQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHVTG 104

Query: 75  TKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRL 134
           TKGKGSTC FTE ILRN G  TG F+SPH++ +RER R++G  IS E F  +FW  YN+L
Sbjct: 105 TKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQL 164

Query: 135 KE-KTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIAS 194
           +E K    V MP  FRFL L+AF +F  E+VD+A++EVG+GG FD TN++R P+VCG++S
Sbjct: 165 EEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSS 224

Query: 195 LGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSL 254
           LG DH  LLG+T+  IA +K GIFK G PAFTV QP+  + VLRD+A Q+   L + P L
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPL 284

Query: 255 D--PELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSR-----SLPEQF 314
           +   E+     LGLEG HQ  NA LA+ L+  WL R  H  +  ++ SR      LP   
Sbjct: 285 EALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAP 344

Query: 315 IEGLTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGN 374
           +   T     G    V    T I     L +YLDGAH+  S++ C  W+  +++ +++  
Sbjct: 345 VFRPTPHMRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWYRQSLERSKR-- 404

Query: 375 LNQQLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHF 434
                  +G S                 V +LLFN    RD   LL  L++ C      F
Sbjct: 405 ------TDGGS----------------EVHILLFNSTGDRDSAALL-KLLQPC-----QF 464

Query: 435 KKALFVPNTSVFDKVGTHGLPEANGQVDLS------------WQFHVRRVWDSIMQCDKG 494
             A+F PN +    +G     + N  V L             W     +   S +    G
Sbjct: 465 DYAVFCPNVTEVSSIG--NADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSNLWSSCG 524

Query: 495 SDSKGTSAVLEEENEKPEVGGKSCENSTVFSSLPMAIKWL---RDSVLQQ---------- 537
            D  G  ++L      P     +  +S VFS +  A+ W+   RD + Q           
Sbjct: 525 PDPAGPGSLL----LAPHPPQPTRTSSLVFSCISHALLWISQGRDPIFQPQSLPRNLLNH 581

BLAST of Lcy09g004560 vs. ExPASy TrEMBL
Match: A0A0A0KTX7 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 6.6e-280
Identity = 480/539 (89.05%), Postives = 511/539 (94.81%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           MVEG  T K+S+  YEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLE+SMSQMK+
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW 
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKE+TSED+PMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLG IASEKAGIFKRGAPAFTVFQPDEAM VL+DKASQLDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+HGRKLGLEGEHQYLNAGLA+ALSS WLHR+GHS+LSYVE SRSLPEQFIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPDRYTD+E P++LVFYLDGAHSPESME CG WFSAA++HNQ GNLN 
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QLENN--GTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFK 420
           Q E+N  GTSSNK LQGYD+DEARKKSVQVLLFNCM+VRDPQLLLPPLIK+CANNGV+FK
Sbjct: 376 QSEDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFK 435

Query: 421 KALFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEE 480
           KALFVPNTSVF+KVGTHGLPE + QVDLSWQFHVRR+W+   QCDKGSDSKGT AVLEEE
Sbjct: 436 KALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEE 495

Query: 481 NEKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           +EK EVGGK+C NSTVFSSLP+AIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 SEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of Lcy09g004560 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.3e-279
Identity = 479/537 (89.20%), Postives = 502/537 (93.48%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           M EG+G  K SRT YEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLE+SMS+MK+
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDH ELLGNTLG IASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+ G KLGLEGEHQYLNA LAIALSS WLHRTG S LSY E S SLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTA FEGRAQIVPDRY DIE+PEDLVFYLDGAHSPESM VC SWF AAIKHNQ+G LN 
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
           QL++NGTSSNKLLQGYD+DEARKKSVQVLLFNCMSVRDPQLLL PLIK+CANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENE 480
           LFVPNTS+F+KVGTHGL EANG+VDLSWQFHVRRVW++I+Q +KG DS+G  A++EEE+E
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. ExPASy TrEMBL
Match: A0A6J1BXX6 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.1e-278
Identity = 476/537 (88.64%), Postives = 503/537 (93.67%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           M  G GT K S+T YEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLE+ M QMK+
Sbjct: 1   MENGNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTS+D+PMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC
Sbjct: 121 CYNRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLG IASEKAGIFKRG PAFTV QPDEAM+VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+HG KLGLEGEHQYLNAGLAIALSSAWL RTGHSKLSY+E SRSLPEQFIEG
Sbjct: 241 VPSLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPDRY DIESP DLVFYLDGAHSPESME CG WFSA +K NQ+G LN 
Sbjct: 301 LTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNY 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
           QLE+NG SSNKLLQ   +DEARK SV VLLFNCMSVRDPQLLLPPLIK+CANNGVHFKKA
Sbjct: 361 QLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENE 480
           LFVPNTSVFDKVGTH L EANGQ+DLSWQF++RRVW++++QC+KG+DSKGT AVLEEE +
Sbjct: 421 LFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKGTDSKGTGAVLEEETD 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVGGKSCENSTVF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. ExPASy TrEMBL
Match: A0A1S3C839 (Folylpolyglutamate synthase OS=Cucumis melo OX=3656 GN=LOC103498061 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.8e-277
Identity = 478/539 (88.68%), Postives = 508/539 (94.25%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           MVEG  T K+S+  YEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLE+SMSQMK+
Sbjct: 1   MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW 
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTSED+PMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 121 CYNRLKEKTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLG IASEKAGIFKR APAFTVFQPDEAM VL+DKASQLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKREAPAFTVFQPDEAMRVLKDKASQLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDP+L+HGRKLGLEGEHQYLNAGLA+ALSS WL RTGHS+LSYVE SR LPEQFIEG
Sbjct: 241 VPSLDPKLLHGRKLGLEGEHQYLNAGLAVALSSTWLRRTGHSQLSYVEQSRCLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPD+YTD+ES +DLVFYLDGAHSPESME CG WFSAA++HNQ GN   
Sbjct: 301 LTTASFEGRAQIVPDKYTDVESSDDLVFYLDGAHSPESMEACGRWFSAAVRHNQLGNFKN 360

Query: 361 QLENN--GTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFK 420
           Q E+N  GTSSNK LQGYD+DEARKKSVQVLLFNCM+VRDPQLLLPPLIK+CANNGV+FK
Sbjct: 361 QSEDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFK 420

Query: 421 KALFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEE 480
           KALFVPNTSVF+KVGTHGL E +GQVDLSWQFHVRR+W++   CDKGSDSKGTSAVLEEE
Sbjct: 421 KALFVPNTSVFNKVGTHGLRETDGQVDLSWQFHVRRIWENTKPCDKGSDSKGTSAVLEEE 480

Query: 481 NEKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           +EK EVGGK+CENSTVFSSLP+AIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 SEKAEVGGKNCENSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 538

BLAST of Lcy09g004560 vs. ExPASy TrEMBL
Match: A0A6J1HBB1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.4e-277
Identity = 474/537 (88.27%), Postives = 502/537 (93.48%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           M EG+G  K SRT YEEALD LSSLIV+RRRCDKSGDRFEL+FEYVKMLDLE+SMS+MK+
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLID+RERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDH ELLGNTLG IASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+ G KLGLEGEHQYLNA LAIALSS WLHRTGHS LSY E S SLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPDRY DIESPEDLVFYLDGAHSPESM VC SWF AAIKHNQ+G L+ 
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
           QL++ GTSSNKLLQGYD+DEARKKSVQVLLFNCMSVRDPQLLL PLIK+C NNGVHFKKA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENE 480
           +FVPNTS+F+KVGTHGL EANG+VDLSWQFHVRRVW++I+Q +KG DS+G  A++EEE+E
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. NCBI nr
Match: XP_004142354.1 (folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein Csa_008061 [Cucumis sativus])

HSP 1 Score: 972.6 bits (2513), Expect = 1.4e-279
Identity = 480/539 (89.05%), Postives = 511/539 (94.81%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           MVEG  T K+S+  YEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLE+SMSQMK+
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW 
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKE+TSED+PMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLG IASEKAGIFKRGAPAFTVFQPDEAM VL+DKASQLDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+HGRKLGLEGEHQYLNAGLA+ALSS WLHR+GHS+LSYVE SRSLPEQFIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPDRYTD+E P++LVFYLDGAHSPESME CG WFSAA++HNQ GNLN 
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QLENN--GTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFK 420
           Q E+N  GTSSNK LQGYD+DEARKKSVQVLLFNCM+VRDPQLLLPPLIK+CANNGV+FK
Sbjct: 376 QSEDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFK 435

Query: 421 KALFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEE 480
           KALFVPNTSVF+KVGTHGLPE + QVDLSWQFHVRR+W+   QCDKGSDSKGT AVLEEE
Sbjct: 436 KALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEE 495

Query: 481 NEKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           +EK EVGGK+C NSTVFSSLP+AIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 SEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of Lcy09g004560 vs. NCBI nr
Match: XP_022990580.1 (folylpolyglutamate synthase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 970.3 bits (2507), Expect = 6.8e-279
Identity = 479/537 (89.20%), Postives = 502/537 (93.48%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           M EG+G  K SRT YEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLE+SMS+MK+
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDH ELLGNTLG IASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+ G KLGLEGEHQYLNA LAIALSS WLHRTG S LSY E S SLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTA FEGRAQIVPDRY DIE+PEDLVFYLDGAHSPESM VC SWF AAIKHNQ+G LN 
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
           QL++NGTSSNKLLQGYD+DEARKKSVQVLLFNCMSVRDPQLLL PLIK+CANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENE 480
           LFVPNTS+F+KVGTHGL EANG+VDLSWQFHVRRVW++I+Q +KG DS+G  A++EEE+E
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. NCBI nr
Match: XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.4 bits (2502), Expect = 2.6e-278
Identity = 478/537 (89.01%), Postives = 502/537 (93.48%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           M EG+G  K SRT YEEALD LSSLIV+RRRCDKSGDRFELMFEYVKMLDLE+SMS+MK+
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDH ELLGNTLG IASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+ G KLGLEGEHQYLNA LAIALSS WLHRTGHS LSY E S SLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPDRY DIESPEDLVFYLDGAHSPESM VC SWF AAIKHNQ+G L+ 
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
           QL++NGTSSNKLLQGYD+DEARKKSVQVLLFNCMSVRDPQLLL PLIK+CANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENE 480
           +FVPNTSVF+KVGTHGL EANG+VDLSWQFHVRRVW++I+Q +KG DS+G   ++EEE+E
Sbjct: 421 VFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGTMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEV GK CENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. NCBI nr
Match: XP_038889970.1 (folylpolyglutamate synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 967.6 bits (2500), Expect = 4.4e-278
Identity = 481/539 (89.24%), Postives = 506/539 (93.88%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           MVEG  T K+SRT YEEALDALS LIVNRRRCDKSGDRFELMFEYVKMLDLE+SM QMK+
Sbjct: 1   MVEGNETLKDSRTAYEEALDALSLLIVNRRRCDKSGDRFELMFEYVKMLDLEESMLQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRN GFHTGLFTSPHLIDIRERFRLDG+DISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNYGFHTGLFTSPHLIDIRERFRLDGVDISEEKFLTYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+AP+VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPVVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLG IASEKAGIFK GAPAFTVFQPDEAM VLRDKASQLDVQLT 
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKHGAPAFTVFQPDEAMRVLRDKASQLDVQLTT 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           V  LDPEL+HGRKLGLEGEHQYLNAGLAIALSS WLHR GHS+LSYVE +RSLPEQFIEG
Sbjct: 241 VSPLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSELSYVEQNRSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPD+Y D+ESP+DLVFYLDGAHSPESME CG WFSAAI+HNQ GNLNQ
Sbjct: 301 LTTASFEGRAQIVPDKYIDVESPDDLVFYLDGAHSPESMEACGRWFSAAIRHNQPGNLNQ 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
             E+N TSSNK LQGYD+DEARKKSVQVLLFNCMSVRDPQLLLPPLIK CANNGV+FKKA
Sbjct: 361 S-EDNATSSNK-LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLPPLIKMCANNGVNFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVW--DSIMQCDKGSDSKGTSAVLEEE 480
           LFVPNTSVF+KVGTHGLPE NGQVDLSWQFH+RRVW  ++ MQC KG+DSKGT  VL+EE
Sbjct: 421 LFVPNTSVFNKVGTHGLPETNGQVDLSWQFHLRRVWEKENTMQCVKGNDSKGTGVVLQEE 480

Query: 481 NEKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           +EK EVGGK+CENSTVFSSLPMAIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 SEKSEVGGKNCENSTVFSSLPMAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. NCBI nr
Match: XP_022134300.1 (folylpolyglutamate synthase isoform X2 [Momordica charantia])

HSP 1 Score: 967.6 bits (2500), Expect = 4.4e-278
Identity = 476/537 (88.64%), Postives = 503/537 (93.67%), Query Frame = 0

Query: 1   MVEGIGTFKNSRTPYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEDSMSQMKI 60
           M  G GT K S+T YEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLE+ M QMK+
Sbjct: 1   MENGNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120

Query: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180
           CYNRLKEKTS+D+PMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC
Sbjct: 121 CYNRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180

Query: 181 GIASLGYDHMELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMELLGNTLG IASEKAGIFKRG PAFTV QPDEAM+VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTV 240

Query: 241 VPSLDPELIHGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEG 300
           VPSLDPEL+HG KLGLEGEHQYLNAGLAIALSSAWL RTGHSKLSY+E SRSLPEQFIEG
Sbjct: 241 VPSLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQ 360
           LTTASFEGRAQIVPDRY DIESP DLVFYLDGAHSPESME CG WFSA +K NQ+G LN 
Sbjct: 301 LTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNY 360

Query: 361 QLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKA 420
           QLE+NG SSNKLLQ   +DEARK SV VLLFNCMSVRDPQLLLPPLIK+CANNGVHFKKA
Sbjct: 361 QLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFDKVGTHGLPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENE 480
           LFVPNTSVFDKVGTH L EANGQ+DLSWQF++RRVW++++QC+KG+DSKGT AVLEEE +
Sbjct: 421 LFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKGTDSKGTGAVLEEETD 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           KPEVGGKSCENSTVF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Lcy09g004560 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 642.5 bits (1656), Expect = 3.0e-184
Identity = 329/526 (62.55%), Postives = 397/526 (75.48%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGKGST 74
           YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLE+ + +M +IHVAGTKGKGST
Sbjct: 67  YEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGST 126

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E+
Sbjct: 127 CTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNEE 186

Query: 135 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           +PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME+
Sbjct: 187 IPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHMEI 246

Query: 195 LGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELIHGR 254
           LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   L+ G+
Sbjct: 247 LGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQ 306

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEGLTTASFEGRAQI 314
           KLGL+GEHQY+NAGLA++L+S WL + G  ++        LPE+FI+GL TAS +GRAQ+
Sbjct: 307 KLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQV 366

Query: 315 VPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQQLENNGTSSNKL 374
           VPD+YT+  +  DLVFYLDGAHSPESME C  WFS A+K + Q   +  L N    S+  
Sbjct: 367 VPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSHD 426

Query: 375 LQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKALFVPNTSVFDKV 434
               ++ E      Q+LLFNCMSVRDP LLLP L   CA  GV+FKKALFVPN SV+ KV
Sbjct: 427 KWSNETCE------QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKV 486

Query: 435 GTHG-LPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENEKPEVGGKSCEN 494
           GT   LPE + QVDLSWQF +++VW+S++Q ++                  E  G+S  N
Sbjct: 487 GTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESDGN 546

Query: 495 STVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           S VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 547 SEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of Lcy09g004560 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 642.5 bits (1656), Expect = 3.0e-184
Identity = 329/526 (62.55%), Postives = 397/526 (75.48%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGKGST 74
           YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLE+ + +M +IHVAGTKGKGST
Sbjct: 9   YEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGST 68

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E+
Sbjct: 69  CTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNEE 128

Query: 135 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           +PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME+
Sbjct: 129 IPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHMEI 188

Query: 195 LGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELIHGR 254
           LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   L+ G+
Sbjct: 189 LGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSGQ 248

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEGLTTASFEGRAQI 314
           KLGL+GEHQY+NAGLA++L+S WL + G  ++        LPE+FI+GL TAS +GRAQ+
Sbjct: 249 KLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQV 308

Query: 315 VPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQQLENNGTSSNKL 374
           VPD+YT+  +  DLVFYLDGAHSPESME C  WFS A+K + Q   +  L N    S+  
Sbjct: 309 VPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSHD 368

Query: 375 LQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKALFVPNTSVFDKV 434
               ++ E      Q+LLFNCMSVRDP LLLP L   CA  GV+FKKALFVPN SV+ KV
Sbjct: 369 KWSNETCE------QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKV 428

Query: 435 GTHG-LPEANGQVDLSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENEKPEVGGKSCEN 494
           GT   LPE + QVDLSWQF +++VW+S++Q ++                  E  G+S  N
Sbjct: 429 GTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESDGN 488

Query: 495 STVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           S VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 489 SEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512

BLAST of Lcy09g004560 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 570.1 bits (1468), Expect = 1.9e-162
Identity = 302/526 (57.41%), Postives = 374/526 (71.10%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LED++SQMKIIHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELIHGR 254
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLA+AL S +L   G    + ++ +  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQQLENNGTSSNKL 374
           VPD     E PE++V+YLDGAHSPESME C  WFS  IK NQ+ N               
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN--------------- 372

Query: 375 LQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKALFVPNTSVFDKV 434
                    +K+S Q+LLFNCMSVRDP LLLP L   C + GV FK+A+FVPN SV+++V
Sbjct: 373 ---------QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 435 GTHGLPEANGQVD-LSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENEKPEVGGKSCEN 494
           G+        +V+ +SWQF ++R+W+S+ + +  S+SK  S             GK  E 
Sbjct: 433 GSS--TNVGTRVESMSWQFGLQRIWESLARGEAKSNSKSDSK------------GKEEEK 491

Query: 495 STVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           S VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of Lcy09g004560 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 563.9 bits (1452), Expect = 1.4e-160
Identity = 301/526 (57.22%), Postives = 373/526 (70.91%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LED++SQMKIIHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATN ++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELIHGR 254
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSRSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLA+AL S +L   G    + ++ +  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYTDIESPEDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLNQQLENNGTSSNKL 374
           VPD     E PE++V+YLDGAHSPESME C  WFS  IK NQ+ N               
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN--------------- 372

Query: 375 LQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKKALFVPNTSVFDKV 434
                    +K+S Q+LLFNCMSVRDP LLLP L   C + GV FK+A+FVPN SV+++V
Sbjct: 373 ---------QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 432

Query: 435 GTHGLPEANGQVD-LSWQFHVRRVWDSIMQCDKGSDSKGTSAVLEEENEKPEVGGKSCEN 494
           G+        +V+ +SWQF ++R+W+S+ + +  S+SK  S             GK  E 
Sbjct: 433 GSS--TNVGTRVESMSWQFGLQRIWESLARGEAKSNSKSDSK------------GKEEEK 490

Query: 495 STVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 538
           S VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 490

BLAST of Lcy09g004560 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 534.6 bits (1376), Expect = 8.9e-152
Identity = 286/551 (51.91%), Postives = 380/551 (68.97%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEDSMSQMKIIHVAGTKGK 74
           Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLED + ++K+IHVAGTKGK
Sbjct: 83  YDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTKGK 142

Query: 75  GSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKT 134
           GSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKEK 
Sbjct: 143 GSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKEKA 202

Query: 135 SEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDH 194
            + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV++ P+VCGIASLG DH
Sbjct: 203 VDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGMDH 262

Query: 195 MELLGNTLGLIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELI 254
           M++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+P+ +
Sbjct: 263 MDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPKKL 322

Query: 255 HGRKLGLEGEHQYLNAGLAIALSSAWLHRTGHSKLSYVEHSR--SLPEQFIEGLTTASFE 314
            G  LGL G+HQ +NAGLA++LS  WL RTG+ K  +   S+   +P  F  GL TA   
Sbjct: 323 DGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATARLH 382

Query: 315 GRAQIVPDRYTD-------IESP-EDLVFYLDGAHSPESMEVCGSWFSAAIKHNQQGNLN 374
           GRAQ+V D  +D       +E+P  DL+FYLDGAHSPESME CG WFS+A++    G+ +
Sbjct: 383 GRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVR----GDKS 442

Query: 375 QQLENNGTSSNKLLQGYDSDEARKKSVQVLLFNCMSVRDPQLLLPPLIKSCANNGVHFKK 434
                NG     +  G    +  + S Q+LLFNCM VRDPQ+LLP L+ +CA++G HF +
Sbjct: 443 LSTAVNG----YMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFSR 502

Query: 435 ALFVPNTSVFDKV--GTHGLPEANGQVDLSWQFHVRRVWD-SIMQCDKGSD----SKGTS 494
           ALFVP+ S ++KV  G   +P    + DL+WQF ++R+W+ SI   D G D      G +
Sbjct: 503 ALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGIT 562

Query: 495 AVLEEE---NEKPEVGGKS---CENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSL 538
           A+   +    + P+ GG +     +S V  SLP+ I WLRD V ++N S++L+VLVTGSL
Sbjct: 563 ALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVTGSL 622