Lcy09g004350 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g004350
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	metalloendoproteinase 2-MMP-like
Location	Chr09: 3928461 .. 3929321 (-)
RNA-Seq Expression	Lcy09g004350
Synteny	Lcy09g004350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCAAATCTCTCCAATTCTTCCTTCTTCTCTTCTCCATTGCACCTCATCTAATCTTCGGACACAATCTCGAATTCCCTCAACATCTTCAAGGACGTCGAAAGGGCGACATCATAGAAGGAATCCACGACGTCAAGACTTACCTTCGCCGATACGGTTACCTAAACAACAAGAAAACCAACTTCCACGCCGACGAAAACGACAACGTTTTCGACGATGGCCTGGAATCCGCCATCAAAACATACCAAAAAACCTTCAACCTCAGCATCACTGGGGTCTTGGACAAAGCGACATTGGCTCAATTTTCCAAACCTCGATGTGCGGTTCCGGATTTCTTCAACCACAAGGAAGCCAATGTCCACATGGGCTCTCGCTACGCTTTCTTCCCAGGAAATCTAAAATGGCCGAGTACAAAATTCCACTTGACCTACAAATTTATTCACGATTACCCATTGAACTTGGTAGCCCCAGTGATTTGGGCTCTGGCGACATGGGCTTCGAACAGCCCGTTCACATTCTCAGAGGCTTTGGAGGGCCAAGTGGCTGACATTAATATCAGCTTTGAGAGAGGGGAGCATGGAGATGGGTCTCCGTTCGATGGAAGAGGAGGAATTTTGGCTCATGCTTTTGCACCGACGGACGGGAGATTGCACTTTGATGCGGACGAGACATGGACGGCGGGGGTTGTTTTGAATGAGTTTGATGTGGAGACTGTGGCACTTCATGAGCTTGGACATGTTCTTGGGCTTGGCCATAGCACCGTCCAACGGGCCATCATGTGGCCGTTTATTGACCCTGGTGCCACCAAGGGCTTGAGTGAAGATGATGTTGCAGGGATCCGTGCTTTATATGGCTAG

mRNA sequence

ATGGCATTCAAATCTCTCCAATTCTTCCTTCTTCTCTTCTCCATTGCACCTCATCTAATCTTCGGACACAATCTCGAATTCCCTCAACATCTTCAAGGACGTCGAAAGGGCGACATCATAGAAGGAATCCACGACGTCAAGACTTACCTTCGCCGATACGGTTACCTAAACAACAAGAAAACCAACTTCCACGCCGACGAAAACGACAACGTTTTCGACGATGGCCTGGAATCCGCCATCAAAACATACCAAAAAACCTTCAACCTCAGCATCACTGGGGTCTTGGACAAAGCGACATTGGCTCAATTTTCCAAACCTCGATGTGCGGTTCCGGATTTCTTCAACCACAAGGAAGCCAATGTCCACATGGGCTCTCGCTACGCTTTCTTCCCAGGAAATCTAAAATGGCCGAGTACAAAATTCCACTTGACCTACAAATTTATTCACGATTACCCATTGAACTTGGTAGCCCCAGTGATTTGGGCTCTGGCGACATGGGCTTCGAACAGCCCGTTCACATTCTCAGAGGCTTTGGAGGGCCAAGTGGCTGACATTAATATCAGCTTTGAGAGAGGGGAGCATGGAGATGGGTCTCCGTTCGATGGAAGAGGAGGAATTTTGGCTCATGCTTTTGCACCGACGGACGGGAGATTGCACTTTGATGCGGACGAGACATGGACGGCGGGGGTTGTTTTGAATGAGTTTGATGTGGAGACTGTGGCACTTCATGAGCTTGGACATGTTCTTGGGCTTGGCCATAGCACCGTCCAACGGGCCATCATGTGGCCGTTTATTGACCCTGGTGCCACCAAGGGCTTGAGTGAAGATGATGTTGCAGGGATCCGTGCTTTATATGGCTAG

Coding sequence (CDS)

ATGGCATTCAAATCTCTCCAATTCTTCCTTCTTCTCTTCTCCATTGCACCTCATCTAATCTTCGGACACAATCTCGAATTCCCTCAACATCTTCAAGGACGTCGAAAGGGCGACATCATAGAAGGAATCCACGACGTCAAGACTTACCTTCGCCGATACGGTTACCTAAACAACAAGAAAACCAACTTCCACGCCGACGAAAACGACAACGTTTTCGACGATGGCCTGGAATCCGCCATCAAAACATACCAAAAAACCTTCAACCTCAGCATCACTGGGGTCTTGGACAAAGCGACATTGGCTCAATTTTCCAAACCTCGATGTGCGGTTCCGGATTTCTTCAACCACAAGGAAGCCAATGTCCACATGGGCTCTCGCTACGCTTTCTTCCCAGGAAATCTAAAATGGCCGAGTACAAAATTCCACTTGACCTACAAATTTATTCACGATTACCCATTGAACTTGGTAGCCCCAGTGATTTGGGCTCTGGCGACATGGGCTTCGAACAGCCCGTTCACATTCTCAGAGGCTTTGGAGGGCCAAGTGGCTGACATTAATATCAGCTTTGAGAGAGGGGAGCATGGAGATGGGTCTCCGTTCGATGGAAGAGGAGGAATTTTGGCTCATGCTTTTGCACCGACGGACGGGAGATTGCACTTTGATGCGGACGAGACATGGACGGCGGGGGTTGTTTTGAATGAGTTTGATGTGGAGACTGTGGCACTTCATGAGCTTGGACATGTTCTTGGGCTTGGCCATAGCACCGTCCAACGGGCCATCATGTGGCCGTTTATTGACCCTGGTGCCACCAAGGGCTTGAGTGAAGATGATGTTGCAGGGATCCGTGCTTTATATGGCTAG

Protein sequence

MAFKSLQFFLLLFSIAPHLIFGHNLEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFFNHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVVLNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALYG

Homology

[Close]

BLAST of Lcy09g004350 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 2.4e-52
Identity = 118/261 (45.21%), Postives = 148/261 (56.70%), Query Frame = 0


Query: 37  GDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLD 96
           G   +G+ +VK Y    GY+ N     H D+N   FDD L SAIKTYQK +NL++TG  D
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAP---HFDDN---FDDTLVSAIKTYQKNYNLNVTGKFD 107

Query: 97  KATLAQFSKPRCAVPDFF--NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLN 156
             TL Q   PRC VPD     +K  +  M S Y FF    +W +    LTY F  +  L+
Sbjct: 108 INTLKQIMTPRCGVPDIIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLD 167

Query: 157 --LVAPVIWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFA 216
               + +  A + W       F E    + A+I I F    HGD  PFDG GGIL HAFA
Sbjct: 168 DTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFA 227

Query: 217 PTDGRLHFDADETW------TAGVVLNEFDVETVALHELGHVLGLGHSTVQRAIMWPFID 276
           PTDGR HFDADE W      T   V + FD+E+VA+HE+GH+LGLGHS+  RAIM+P I 
Sbjct: 228 PTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIP 287

Query: 277 PGATK-GLSEDDVAGIRALYG 287
           P   K  L++DD+ GIR LYG
Sbjct: 288 PRTRKVNLAQDDIDGIRKLYG 302

[Close]

BLAST of Lcy09g004350 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 7.8e-51
Identity = 119/273 (43.59%), Postives = 151/273 (55.31%), Query Frame = 0


Query: 31  LQGRRKGDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLS 90
           L G   G+ I G+  +K Y RR+GY+    T    D+    FDD L+SAI TYQK FNL 
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITT--TGNCTDD----FDDVLQSAINTYQKNFNLK 107

Query: 91  ITGVLDKATLAQFSKPRCAVPDFFNH-KEAN----VHMGSRYAFFPGNLKWPSTKFHLTY 150
           +TG LD +TL Q  KPRC  PD  +   E N    +    +Y+FFPG  +WP  K  LTY
Sbjct: 108 VTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTY 167

Query: 151 KFIHDYPLNLVAPVIW--ALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGR 210
            F     L      ++  A   WA  +P  F+ +     ADI I F  GEHGDG PFDG 
Sbjct: 168 AFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGA 227

Query: 211 GGILAHAFAPTDGRLHFDADETWTAG---------VVLNEFDVETVALHELGHVLGLGHS 270
            G LAHA +P  G LH D DE W             V    D+E+VA+HE+GH+LGLGHS
Sbjct: 228 MGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHS 287

Query: 271 TVQRAIMWPFIDPGATK-GLSEDDVAGIRALYG 287
           +V+ AIM+P I  G  K  L++DD+ GI+ LYG
Sbjct: 288 SVEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

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BLAST of Lcy09g004350 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.3e-49
Identity = 106/280 (37.86%), Postives = 149/280 (53.21%), Query Frame = 0


Query: 33  GRRKGDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSIT 92
           G   G  ++G++ +K Y +R+GY+    +    D+    FDD L++A++ YQ  FNL++T
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDD----FDDILKAAVELYQTNFNLNVT 108

Query: 93  GVLDKATLAQFSKPRCAVPDF----------------FNHKEANVHMGSRYAFFPGNLKW 152
           G LD  T+     PRC  PD                  N    ++H   RY  FPG  +W
Sbjct: 109 GELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRW 168

Query: 153 PSTKFHLTYKFIHDYPLNLVAPVIW--ALATWASNSPFTFSEALEGQVADINISFERGEH 212
           P  +  LTY F    PL      ++  A   W+  +   F+ +     +DI I F  G+H
Sbjct: 169 PRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDH 228

Query: 213 GDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVVLNEF-------DVETVALHELGH 272
           GDG PFDG  G LAHAF+P  G+ H DADE W     L+ F       D+E+VA+HE+GH
Sbjct: 229 GDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGH 288

Query: 273 VLGLGHSTVQRAIMWPFIDPGATK-GLSEDDVAGIRALYG 287
           +LGLGHS+V+ +IM+P I  G  K  L+ DDV GI+ LYG
Sbjct: 289 LLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Lcy09g004350 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.4e-47
Identity = 108/256 (42.19%), Postives = 143/256 (55.86%), Query Frame = 0


Query: 43  IHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQ 102
           I ++K +L++YGYL   K      E+D+V     E A+  YQK   L ITG  D  TL+Q
Sbjct: 50  IPEIKRHLQQYGYLPQNK------ESDDV---SFEQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 103 FSKPRCAVPDFFNHKEANVHMGSRYAFFPGNLKWP-STKFHLTYKFIHDYPLNLVAPV-- 162
              PRC  PD    K A  H G +Y +FPG  +W       LTY F  +     +AP   
Sbjct: 110 ILLPRCGFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDI 169

Query: 163 ----IWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTD 222
                 A   WAS  P +F E  +  +ADI I F  G+HGDG PFDG  G+LAH F+P +
Sbjct: 170 RRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPEN 229

Query: 223 GRLHFDADETWTAGVVLNE----FDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATK 282
           GRLH D  ETW       +     D+E+VA+HE+GHVLGLGHS+V+ A M+P + P + K
Sbjct: 230 GRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKK 289

Query: 283 -GLSEDDVAGIRALYG 287
             L+ DDV G+++LYG
Sbjct: 290 VNLNMDDVVGVQSLYG 296

[Close]

BLAST of Lcy09g004350 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-46
Identity = 110/265 (41.51%), Postives = 146/265 (55.09%), Query Frame = 0


Query: 37  GDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLD 96
           G  + G+ ++K YL R+GY+N+    F      +VFD  LESAI  YQ+   L ITG LD
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIF-----SDVFDGPLESAISLYQENLGLPITGRLD 123

Query: 97  KATLAQFSKPRCAVPDFFNHKEAN---VHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPL 156
            +T+   S PRC V D   H   N   +H  + Y +F G  KW   +  LTY     + L
Sbjct: 124 TSTVTLMSLPRCGVSD--THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKL 183

Query: 157 NLVAP------VIWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGIL 216
           + +           A + W+S  P +F E  +   AD+ I F  G+HGDG PFDG  G L
Sbjct: 184 DYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTL 243

Query: 217 AHAFAPTDGRLHFDADETWTAGVVLN-----EFDVETVALHELGHVLGLGHSTVQRAIMW 276
           AHAFAP +GRLH DA ETW     L        D+E+VA HE+GH+LGLGHS+ + A+M+
Sbjct: 244 AHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMY 303

Query: 277 PFIDPGATK-GLSEDDVAGIRALYG 287
           P + P   K  L+ DDVAG+  LYG
Sbjct: 304 PSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Lcy09g004350 vs. ExPASy TrEMBL
Match: A0A6J1BZK9 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006143 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 5.7e-105
Identity = 187/290 (64.48%), Postives = 225/290 (77.59%), Query Frame = 0


Query: 1   MAFKSLQFFLLLF-SIAPHLIFG--HNLEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYLN 60
           MAFK L+ FLLL  S+AP +I    H+++FP+HLQG RKGD +EGI +VK +L+RYGY+ 
Sbjct: 1   MAFKCLKLFLLLLASVAPRVILSHDHHIQFPEHLQGCRKGDNVEGIQNVKMHLQRYGYMR 60

Query: 61  NKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF--N 120
               N    E+ N FD+ LESAIK YQK FNL+++G LD+ TL   S+PRC VPD    +
Sbjct: 61  KDNNNTVNKESTNAFDEALESAIKKYQKNFNLNLSGALDRETLDLMSRPRCGVPDIVASS 120

Query: 121 HKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSPFTFS 180
           + E  +   S YAFFPGNLKWP+TK+HLTY FI++YP N VA V  ALATWA  S FTF+
Sbjct: 121 NNETMMWNSSHYAFFPGNLKWPNTKYHLTYTFINNYPSNYVAAVARALATWAGASQFTFA 180

Query: 181 EALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVVLNEF 240
           EA EG+ ADI ISFERG HGDG+PFDGRGG+LAHAFAP+DGRLH DADE+WT GVV NEF
Sbjct: 181 EAFEGETADIKISFERGNHGDGNPFDGRGGVLAHAFAPSDGRLHLDADESWTEGVVFNEF 240

Query: 241 DVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
           DVETVALHELGH+LGLGHS ++ AIMWPF+DPGATKGL+ DD+ GI ALY
Sbjct: 241 DVETVALHELGHILGLGHSKIEAAIMWPFLDPGATKGLNADDIDGIHALY 290

[Close]

BLAST of Lcy09g004350 vs. ExPASy TrEMBL
Match: A0A5A7T3V3 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00350 PE=3 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 9.2e-87
Identity = 163/294 (55.44%), Postives = 206/294 (70.07%), Query Frame = 0


Query: 1   MAFKSLQ-FFLLLFSIAPHLIFGH-NLEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYL-N 60
           MAFKSLQ FFLLL S   H+I  H     PQHL G RKGD +EGIH +K YL RYGYL +
Sbjct: 1   MAFKSLQLFFLLLASFTSHVISRHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLSH 60

Query: 61  NKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF--- 120
           N  T+ H + N N FDD LESA+K YQK   L+++G+LD+ TL Q  KPRC +PD     
Sbjct: 61  NTNTSSHIELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMKS 120

Query: 121 ---NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSP 180
               + E ++ + S YA FPGN KWP  K HLTY F +++P++ V  V  A+  WA++S 
Sbjct: 121 NSNKNTEDDLEISSHYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKEWAAHSL 180

Query: 181 FTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVV 240
           FTFS+A + QV DINISF+ G+HGDG PFDG GG++ HAFAPTDGRLH D D+ W+AG+ 
Sbjct: 181 FTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDKWSAGLE 240

Query: 241 LNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
             + +V   ALHELGHVLGL HSTVQ+A+MWP+ID  A K L++DD+AG+ ALY
Sbjct: 241 TQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIAGLTALY 294

[Close]

BLAST of Lcy09g004350 vs. ExPASy TrEMBL
Match: A0A1S3C9J6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498179 PE=3 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 9.2e-87
Identity = 163/294 (55.44%), Postives = 206/294 (70.07%), Query Frame = 0


Query: 1   MAFKSLQ-FFLLLFSIAPHLIFGH-NLEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYL-N 60
           MAFKSLQ FFLLL S   H+I  H     PQHL G RKGD +EGIH +K YL RYGYL +
Sbjct: 1   MAFKSLQLFFLLLASFTSHVISRHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLSH 60

Query: 61  NKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF--- 120
           N  T+ H + N N FDD LESA+K YQK   L+++G+LD+ TL Q  KPRC +PD     
Sbjct: 61  NTNTSSHIELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMKS 120

Query: 121 ---NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSP 180
               + E ++ + S YA FPGN KWP  K HLTY F +++P++ V  V  A+  WA++S 
Sbjct: 121 NSNKNTEDDLEISSHYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKEWAAHSL 180

Query: 181 FTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVV 240
           FTFS+A + QV DINISF+ G+HGDG PFDG GG++ HAFAPTDGRLH D D+ W+AG+ 
Sbjct: 181 FTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDKWSAGLE 240

Query: 241 LNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
             + +V   ALHELGHVLGL HSTVQ+A+MWP+ID  A K L++DD+AG+ ALY
Sbjct: 241 TQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIAGLTALY 294

[Close]

BLAST of Lcy09g004350 vs. ExPASy TrEMBL
Match: A0A6J1BYZ9 (metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006521 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.7e-86
Identity = 166/314 (52.87%), Postives = 205/314 (65.29%), Query Frame = 0


Query: 1   MAFKSLQFF---LLLFSIAP------------HLIFGHNLEFPQHLQGRRKGDIIEGIHD 60
           MA K L FF   LLL +I P            H        FP HL G +KGD +EGIH 
Sbjct: 1   MALKGLSFFTFTLLLLTIFPLMSSSRPNPAHSHRKMQSQFVFPDHLHGSQKGDKLEGIHQ 60

Query: 61  VKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSK 120
           +K YL+++GYLNN     H++ N++ FD+ LE AIKTYQK +NL +TG LD  TLAQ SK
Sbjct: 61  LKKYLQQFGYLNN--VQIHSETNEDEFDEFLEFAIKTYQKNYNLKVTGTLDAMTLAQMSK 120

Query: 121 PRCAVPDFFNHK--------------EANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDY 180
           PRC V D  + K               ++ H  S +AFF GN KWP+TK HLTY FI   
Sbjct: 121 PRCGVADIIDGKTWMRSGKKRNEGDGSSHFHTVSHFAFFEGNPKWPTTKSHLTYGFISGT 180

Query: 181 PLNLVAPVIWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAF 240
           PL  + PV  A +TWA+NS FTFS+    Q ADINISF+RG+HGDG PFDG GG++AHAF
Sbjct: 181 PLEAITPVGRAFSTWAANSHFTFSQVSGNQTADINISFQRGDHGDGFPFDGVGGVVAHAF 240

Query: 241 APTDGRLHFDADETWTAGVVLNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATK 286
           +P+DGR H DADE+W AGV    FD+ETVALHE+GH+LGL HS+++ AIMWP +  GA K
Sbjct: 241 SPSDGRFHLDADESWAAGVASGSFDLETVALHEIGHLLGLQHSSIEGAIMWPSVPEGAAK 300

[Close]

BLAST of Lcy09g004350 vs. ExPASy TrEMBL
Match: A0A0A0KNY8 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 1.1e-84
Identity = 164/294 (55.78%), Postives = 208/294 (70.75%), Query Frame = 0


Query: 1   MAFKSLQ-FFLLLFSIAPHLIFGHN-LEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYL-N 60
           MAFKSLQ  FL+L +IA H+   HN +  PQ L G RKGD +EGIH +K YL+RYGYL +
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NKKTNFHADE-NDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF-- 120
           N   + H  E N N FDD LESAIK YQK  +L+++G+LD+ TL Q  KPRC V D F  
Sbjct: 61  NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKF 120

Query: 121 ---NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSP 180
               + E ++ M S Y  FPGNLKWP  K HLTY F +++P++ V  V  A+A WA+ S 
Sbjct: 121 NSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPVDFVPSVTEAMARWAAQSL 180

Query: 181 FTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVV 240
           FTFSEA + Q ADINISF+  +H DG PFDG GG++ HAFAPTDGRLH D D+TW+AG+ 
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGME 240

Query: 241 LNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
           + + +V  VALHELGHVLGL HST+ +A+MWP+ID  A K L++DD+AG+ ALY
Sbjct: 241 VQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALY 294

[Close]

BLAST of Lcy09g004350 vs. NCBI nr
Match: XP_022133603.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 390.6 bits (1002), Expect = 1.2e-104
Identity = 187/290 (64.48%), Postives = 225/290 (77.59%), Query Frame = 0


Query: 1   MAFKSLQFFLLLF-SIAPHLIFG--HNLEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYLN 60
           MAFK L+ FLLL  S+AP +I    H+++FP+HLQG RKGD +EGI +VK +L+RYGY+ 
Sbjct: 1   MAFKCLKLFLLLLASVAPRVILSHDHHIQFPEHLQGCRKGDNVEGIQNVKMHLQRYGYMR 60

Query: 61  NKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF--N 120
               N    E+ N FD+ LESAIK YQK FNL+++G LD+ TL   S+PRC VPD    +
Sbjct: 61  KDNNNTVNKESTNAFDEALESAIKKYQKNFNLNLSGALDRETLDLMSRPRCGVPDIVASS 120

Query: 121 HKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSPFTFS 180
           + E  +   S YAFFPGNLKWP+TK+HLTY FI++YP N VA V  ALATWA  S FTF+
Sbjct: 121 NNETMMWNSSHYAFFPGNLKWPNTKYHLTYTFINNYPSNYVAAVARALATWAGASQFTFA 180

Query: 181 EALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVVLNEF 240
           EA EG+ ADI ISFERG HGDG+PFDGRGG+LAHAFAP+DGRLH DADE+WT GVV NEF
Sbjct: 181 EAFEGETADIKISFERGNHGDGNPFDGRGGVLAHAFAPSDGRLHLDADESWTEGVVFNEF 240

Query: 241 DVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
           DVETVALHELGH+LGLGHS ++ AIMWPF+DPGATKGL+ DD+ GI ALY
Sbjct: 241 DVETVALHELGHILGLGHSKIEAAIMWPFLDPGATKGLNADDIDGIHALY 290

[Close]

BLAST of Lcy09g004350 vs. NCBI nr
Match: XP_038889211.1 (metalloendoproteinase 1-like [Benincasa hispida])

HSP 1 Score: 384.4 bits (986), Expect = 8.5e-103
Identity = 185/295 (62.71%), Postives = 234/295 (79.32%), Query Frame = 0


Query: 1   MAFKSLQ-FFLLLFSIAPHLIFGHN-LEFPQHLQGRRKGDIIEGIHDVKTYLRRYGY-LN 60
           MAFKSLQ  FL L + APH+I   N + FPQHL G RKGD ++GIH++KTYL+ YGY L+
Sbjct: 1   MAFKSLQLLFLFLTTTAPHVISKQNSISFPQHLHGCRKGDNVKGIHNIKTYLQHYGYLLH 60

Query: 61  NKKTNFHADE-NDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF-- 120
           N  T+FH ++  +++F+D LESAIK+YQK FNL+++GVLD+ TL Q S+PRC VPDF   
Sbjct: 61  NTSTDFHTNKLKNDIFNDALESAIKSYQKRFNLNMSGVLDEETLVQISRPRCGVPDFSMS 120

Query: 121 ---NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSP 180
               + + ++ M S Y FFP N KWP+TKFHLTY F ++YPLNLV  V  A+ATWA NS 
Sbjct: 121 NPNKNPKDDLKMSSHYTFFPNNPKWPNTKFHLTYTFTNNYPLNLVPSVTEAMATWALNSQ 180

Query: 181 FTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVV 240
           FTFSE ++GQ+ADINISF+RGEHGDG+PFDG GG+  HA APTDGRLHFD DE+W+AGVV
Sbjct: 181 FTFSEVVDGQIADINISFQRGEHGDGNPFDGPGGMFDHASAPTDGRLHFDGDESWSAGVV 240

Query: 241 LNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALYG 287
            NE +V TVALHELGHVLGLGHS++Q+AIMWP+++PG  KGL++DD+ G+RALYG
Sbjct: 241 DNELNVMTVALHELGHVLGLGHSSIQQAIMWPYMNPGTIKGLNDDDIVGLRALYG 295

[Close]

BLAST of Lcy09g004350 vs. NCBI nr
Match: XP_008458917.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo] >KAA0037468.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01944.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 330.1 bits (845), Expect = 1.9e-86
Identity = 163/294 (55.44%), Postives = 206/294 (70.07%), Query Frame = 0


Query: 1   MAFKSLQ-FFLLLFSIAPHLIFGH-NLEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYL-N 60
           MAFKSLQ FFLLL S   H+I  H     PQHL G RKGD +EGIH +K YL RYGYL +
Sbjct: 1   MAFKSLQLFFLLLASFTSHVISRHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLSH 60

Query: 61  NKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF--- 120
           N  T+ H + N N FDD LESA+K YQK   L+++G+LD+ TL Q  KPRC +PD     
Sbjct: 61  NTNTSSHIELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMKS 120

Query: 121 ---NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSP 180
               + E ++ + S YA FPGN KWP  K HLTY F +++P++ V  V  A+  WA++S 
Sbjct: 121 NSNKNTEDDLEISSHYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKEWAAHSL 180

Query: 181 FTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVV 240
           FTFS+A + QV DINISF+ G+HGDG PFDG GG++ HAFAPTDGRLH D D+ W+AG+ 
Sbjct: 181 FTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDKWSAGLE 240

Query: 241 LNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
             + +V   ALHELGHVLGL HSTVQ+A+MWP+ID  A K L++DD+AG+ ALY
Sbjct: 241 TQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIAGLTALY 294

[Close]

BLAST of Lcy09g004350 vs. NCBI nr
Match: XP_022134202.1 (metalloendoproteinase 2-MMP-like [Momordica charantia])

HSP 1 Score: 328.6 bits (841), Expect = 5.5e-86
Identity = 166/314 (52.87%), Postives = 205/314 (65.29%), Query Frame = 0


Query: 1   MAFKSLQFF---LLLFSIAP------------HLIFGHNLEFPQHLQGRRKGDIIEGIHD 60
           MA K L FF   LLL +I P            H        FP HL G +KGD +EGIH 
Sbjct: 1   MALKGLSFFTFTLLLLTIFPLMSSSRPNPAHSHRKMQSQFVFPDHLHGSQKGDKLEGIHQ 60

Query: 61  VKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSK 120
           +K YL+++GYLNN     H++ N++ FD+ LE AIKTYQK +NL +TG LD  TLAQ SK
Sbjct: 61  LKKYLQQFGYLNN--VQIHSETNEDEFDEFLEFAIKTYQKNYNLKVTGTLDAMTLAQMSK 120

Query: 121 PRCAVPDFFNHK--------------EANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDY 180
           PRC V D  + K               ++ H  S +AFF GN KWP+TK HLTY FI   
Sbjct: 121 PRCGVADIIDGKTWMRSGKKRNEGDGSSHFHTVSHFAFFEGNPKWPTTKSHLTYGFISGT 180

Query: 181 PLNLVAPVIWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAF 240
           PL  + PV  A +TWA+NS FTFS+    Q ADINISF+RG+HGDG PFDG GG++AHAF
Sbjct: 181 PLEAITPVGRAFSTWAANSHFTFSQVSGNQTADINISFQRGDHGDGFPFDGVGGVVAHAF 240

Query: 241 APTDGRLHFDADETWTAGVVLNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATK 286
           +P+DGR H DADE+W AGV    FD+ETVALHE+GH+LGL HS+++ AIMWP +  GA K
Sbjct: 241 SPSDGRFHLDADESWAAGVASGSFDLETVALHEIGHLLGLQHSSIEGAIMWPSVPEGAAK 300

[Close]

BLAST of Lcy09g004350 vs. NCBI nr
Match: XP_004142843.3 (metalloendoproteinase 1 [Cucumis sativus] >KAE8648550.1 hypothetical protein Csa_008112 [Cucumis sativus])

HSP 1 Score: 320.5 bits (820), Expect = 1.5e-83
Identity = 161/294 (54.76%), Postives = 207/294 (70.41%), Query Frame = 0


Query: 1   MAFKSLQ-FFLLLFSIAPHLIFGHN-LEFPQHLQGRRKGDIIEGIHDVKTYLRRYGYL-N 60
           MAFKSLQ  FL+L +IA H+   HN +  PQ L G RKGD +EGIH +K YL+RYGYL +
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NKKTNFHADE-NDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQFSKPRCAVPDFF-- 120
           N   + H  E N N FDD LESAIK YQK  +L+++G+LD+ TL Q  KPRC V D F  
Sbjct: 61  NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKF 120

Query: 121 ---NHKEANVHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPLNLVAPVIWALATWASNSP 180
               + E ++ M S Y  FPGNLKWP  K HLTY F +++P++ +  V  A+A WA+ S 
Sbjct: 121 NSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPIDFMPSVTEAMARWAAQSL 180

Query: 181 FTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVV 240
           FTFSEA + Q ADINISF+  +H DG PFDG GG++ HAFAPTDGRLH D D+TW+AG+ 
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGME 240

Query: 241 LNEFDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATKGLSEDDVAGIRALY 286
           + + +V   ALHELGH+LGL HST+ +A+MWP+ID  A K L++DD+AG+ ALY
Sbjct: 241 VQKVNVMNAALHELGHILGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALY 294

[Close]

BLAST of Lcy09g004350 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 202.2 bits (513), Expect = 5.5e-52
Identity = 119/273 (43.59%), Postives = 151/273 (55.31%), Query Frame = 0


Query: 31  LQGRRKGDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLS 90
           L G   G+ I G+  +K Y RR+GY+    T    D+    FDD L+SAI TYQK FNL 
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITT--TGNCTDD----FDDVLQSAINTYQKNFNLK 107

Query: 91  ITGVLDKATLAQFSKPRCAVPDFFNH-KEAN----VHMGSRYAFFPGNLKWPSTKFHLTY 150
           +TG LD +TL Q  KPRC  PD  +   E N    +    +Y+FFPG  +WP  K  LTY
Sbjct: 108 VTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTY 167

Query: 151 KFIHDYPLNLVAPVIW--ALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGR 210
            F     L      ++  A   WA  +P  F+ +     ADI I F  GEHGDG PFDG 
Sbjct: 168 AFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGA 227

Query: 211 GGILAHAFAPTDGRLHFDADETWTAG---------VVLNEFDVETVALHELGHVLGLGHS 270
            G LAHA +P  G LH D DE W             V    D+E+VA+HE+GH+LGLGHS
Sbjct: 228 MGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHS 287

Query: 271 TVQRAIMWPFIDPGATK-GLSEDDVAGIRALYG 287
           +V+ AIM+P I  G  K  L++DD+ GI+ LYG
Sbjct: 288 SVEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lcy09g004350 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 196.4 bits (498), Expect = 3.0e-50
Identity = 106/280 (37.86%), Postives = 149/280 (53.21%), Query Frame = 0


Query: 33  GRRKGDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSIT 92
           G   G  ++G++ +K Y +R+GY+    +    D+    FDD L++A++ YQ  FNL++T
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDD----FDDILKAAVELYQTNFNLNVT 108

Query: 93  GVLDKATLAQFSKPRCAVPDF----------------FNHKEANVHMGSRYAFFPGNLKW 152
           G LD  T+     PRC  PD                  N    ++H   RY  FPG  +W
Sbjct: 109 GELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRW 168

Query: 153 PSTKFHLTYKFIHDYPLNLVAPVIW--ALATWASNSPFTFSEALEGQVADINISFERGEH 212
           P  +  LTY F    PL      ++  A   W+  +   F+ +     +DI I F  G+H
Sbjct: 169 PRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDH 228

Query: 213 GDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAGVVLNEF-------DVETVALHELGH 272
           GDG PFDG  G LAHAF+P  G+ H DADE W     L+ F       D+E+VA+HE+GH
Sbjct: 229 GDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGH 288

Query: 273 VLGLGHSTVQRAIMWPFIDPGATK-GLSEDDVAGIRALYG 287
           +LGLGHS+V+ +IM+P I  G  K  L+ DDV GI+ LYG
Sbjct: 289 LLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Lcy09g004350 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 191.4 bits (485), Expect = 9.8e-49
Identity = 108/256 (42.19%), Postives = 143/256 (55.86%), Query Frame = 0


Query: 43  IHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLDKATLAQ 102
           I ++K +L++YGYL   K      E+D+V     E A+  YQK   L ITG  D  TL+Q
Sbjct: 50  IPEIKRHLQQYGYLPQNK------ESDDV---SFEQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 103 FSKPRCAVPDFFNHKEANVHMGSRYAFFPGNLKWP-STKFHLTYKFIHDYPLNLVAPV-- 162
              PRC  PD    K A  H G +Y +FPG  +W       LTY F  +     +AP   
Sbjct: 110 ILLPRCGFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDI 169

Query: 163 ----IWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGILAHAFAPTD 222
                 A   WAS  P +F E  +  +ADI I F  G+HGDG PFDG  G+LAH F+P +
Sbjct: 170 RRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPEN 229

Query: 223 GRLHFDADETWTAGVVLNE----FDVETVALHELGHVLGLGHSTVQRAIMWPFIDPGATK 282
           GRLH D  ETW       +     D+E+VA+HE+GHVLGLGHS+V+ A M+P + P + K
Sbjct: 230 GRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKK 289

Query: 283 -GLSEDDVAGIRALYG 287
             L+ DDV G+++LYG
Sbjct: 290 VNLNMDDVVGVQSLYG 296

[Close]

BLAST of Lcy09g004350 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-47
Identity = 110/265 (41.51%), Postives = 146/265 (55.09%), Query Frame = 0


Query: 37  GDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSITGVLD 96
           G  + G+ ++K YL R+GY+N+    F      +VFD  LESAI  YQ+   L ITG LD
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIF-----SDVFDGPLESAISLYQENLGLPITGRLD 123

Query: 97  KATLAQFSKPRCAVPDFFNHKEAN---VHMGSRYAFFPGNLKWPSTKFHLTYKFIHDYPL 156
            +T+   S PRC V D   H   N   +H  + Y +F G  KW   +  LTY     + L
Sbjct: 124 TSTVTLMSLPRCGVSD--THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKL 183

Query: 157 NLVAP------VIWALATWASNSPFTFSEALEGQVADINISFERGEHGDGSPFDGRGGIL 216
           + +           A + W+S  P +F E  +   AD+ I F  G+HGDG PFDG  G L
Sbjct: 184 DYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTL 243

Query: 217 AHAFAPTDGRLHFDADETWTAGVVLN-----EFDVETVALHELGHVLGLGHSTVQRAIMW 276
           AHAFAP +GRLH DA ETW     L        D+E+VA HE+GH+LGLGHS+ + A+M+
Sbjct: 244 AHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMY 303

Query: 277 PFIDPGATK-GLSEDDVAGIRALYG 287
           P + P   K  L+ DDVAG+  LYG
Sbjct: 304 PSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Lcy09g004350 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.4e-47
Identity = 105/282 (37.23%), Postives = 147/282 (52.13%), Query Frame = 0


Query: 33  GRRKGDIIEGIHDVKTYLRRYGYLNNKKTNFHADENDNVFDDGLESAIKTYQKTFNLSIT 92
           G   G   +G++ +K Y + +GY+   +TN   +  D+ FDD L++A++ YQ+ F L++T
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYIT--ETNLSGNFTDD-FDDILKNAVEMYQRNFQLNVT 110

Query: 93  GVLDKATLAQFSKPRCAVPDFFN-----------------HKEANVHMGSRYAFFPGNLK 152
           GVLD+ TL     PRC  PD  N                  +    H    Y+FFPG  +
Sbjct: 111 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 170

Query: 153 WPSTKFHLTYKFIHDYPLNLVAPVIW--ALATWASNSPFTFSEALEGQVADINISFERGE 212
           WP  +  LTY F     L      ++  A   W   +P TF+       +DI+I F  GE
Sbjct: 171 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 230

Query: 213 HGDGSPFDGRGGILAHAFAPTDGRLHFDADETWTAG--------VVLNEFDVETVALHEL 272
           HGDG PFDG    LAHAF+P  G  H D +E W            V    D+E+VA+HE+
Sbjct: 231 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 290

Query: 273 GHVLGLGHSTVQRAIMWPFIDPGATK-GLSEDDVAGIRALYG 287
           GH+LGLGHS+V+ +IM+P I  G  K  L+ DDV G++ LYG
Sbjct: 291 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

The following BLAST results are available for this feature:

BLAST of Lcy09g004350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29136	2.4e-52	45.21	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q9ZUJ5	7.8e-51	43.59	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O04529	4.3e-49	37.86	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q8GWW6	1.4e-47	42.19	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]
O23507	2.6e-46	41.51	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Lcy09g004350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZK9	5.7e-105	64.48	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006143 PE=3...	[more]
A0A5A7T3V3	9.2e-87	55.44	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C9J6	9.2e-87	55.44	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498179 PE=3 SV...	[more]
A0A6J1BYZ9	2.7e-86	52.87	metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006521 ...	[more]
A0A0A0KNY8	1.1e-84	55.78	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G522910 PE=3 S...	[more]

BLAST of Lcy09g004350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133603.1	1.2e-104	64.48	metalloendoproteinase 1-like [Momordica charantia]	[more]
XP_038889211.1	8.5e-103	62.71	metalloendoproteinase 1-like [Benincasa hispida]	[more]
XP_008458917.1	1.9e-86	55.44	PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo] >KAA0037468.1 metallo...	[more]
XP_022134202.1	5.5e-86	52.87	metalloendoproteinase 2-MMP-like [Momordica charantia]	[more]
XP_004142843.3	1.5e-83	54.76	metalloendoproteinase 1 [Cucumis sativus] >KAE8648550.1 hypothetical protein Csa...	[more]

BLAST of Lcy09g004350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59970.1	5.5e-52	43.59	Matrixin family protein	[more]
AT1G70170.1	3.0e-50	37.86	matrix metalloproteinase	[more]
AT2G45040.1	9.8e-49	42.19	Matrixin family protein	[more]
AT4G16640.1	1.8e-47	41.51	Matrixin family protein	[more]
AT1G24140.1	5.4e-47	37.23	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 103..116 score: 45.82 coord: 240..265 score: 57.97 coord: 273..286 score: 61.5 coord: 159..174 score: 32.09 coord: 183..211 score: 65.56
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 132..286 e-value: 2.2E-30 score: 117.0
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 36..286 e-value: 8.7E-76 score: 256.6
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 142..286 e-value: 1.4E-43 score: 148.5
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 42..102 e-value: 4.2E-13 score: 49.3
None	No IPR available	PIRSR	PIRSR001191-1	PIRSR001191-1	coord: 44..286 e-value: 1.3E-63 score: 213.1
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 27..286
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 27..286
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 129..286
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 135..286 e-value: 1.29807E-58 score: 182.789
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 41..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g004350.1	Lcy09g004350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity