Lcy09g003330 (gene) Sponge gourd (P93075) v1

BLAST of Lcy09g003330 vs. ExPASy Swiss-Prot
Match: Q9SGA8 (UDP-glycosyltransferase 83A1 OS=Arabidopsis thaliana OX=3702 GN=UGT83A1 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.3e-89
Identity = 169/454 (37.22%), Postives = 259/454 (57.05%), Query Frame = 0

Query: 9   VIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQ-ILGPNIEL 68
           V+VIP+P QGHV PL+  S++LAKQGI+IT INT+F HN+++ ++ ++ H+  +G  I L
Sbjct: 14  VVVIPYPAQGHVLPLISFSRYLAKQGIQITFINTEFNHNRIISSLPNSPHEDYVGDQINL 73

Query: 69  TSLPDGFPPHEKRDGFRQLFVELPINLKP-QFETLLAKMNADEDA---ISCAIVDMSMAW 128
            S+PDG     +         E  +   P + E L+ +M A+      ISC + D S+ W
Sbjct: 74  VSIPDGLEDSPEERNIPGKLSESVLRFMPKKVEELIERMMAETSGGTIISCVVADQSLGW 133

Query: 129 VLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKSLLGCPPL 188
            +E+A + GIR + + P  AA  V+ FSI +LI++G+ID +G  ++ N  ++   G P +
Sbjct: 134 AIEVAAKFGIRRTAFCPAAAASMVLGFSIQKLIDDGLIDSDGT-VRVNKTIQLSPGMPKM 193

Query: 189 YTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKLLTLGP 248
            T  F W C+  K +QK +F  +++         + +    H LE     L P ++ +GP
Sbjct: 194 ETDKFVWVCLKNKESQKNIFQLMLQNNNSIESTDWLLCNSVHELETAAFGLGPNIVPIGP 253

Query: 249 L--------ISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGME 308
           +         ST  G     D  CL+WLDRQ PGSV+Y+AFGS G     Q EELA+G+E
Sbjct: 254 IGWAHSLEEGSTSLGSFLPHDRDCLDWLDRQIPGSVIYVAFGSFGVMGNPQLEELAIGLE 313

Query: 309 MTRCPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGV 368
           +T+ P LWV    T  Q P +   +R K+V W PQ++VL   A+G F+SHCGWNS +EG 
Sbjct: 314 LTKRPVLWV----TGDQQPIKLGSDRVKVVRWAPQREVLSSGAIGCFVSHCGWNSTLEGA 373

Query: 369 TSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKA 428
            +G+ FLC+P   D  +NK Y+ ++W++G+GLE+D  G+V R E+ +K+ ++M D     
Sbjct: 374 QNGIPFLCIPYFADQFINKAYICDVWKIGLGLERDARGVVPRLEVKKKIDEIMRDGGEYE 433

Query: 429 RSRAFSEEIGKCWEVPHGRSVQNFDRLVRWIKQE 450
                 +EI        G S +N ++ V WIK +
Sbjct: 434 ERAMKVKEIVMKSVAKDGISCENLNKFVNWIKSQ 462

BLAST of Lcy09g003330 vs. ExPASy Swiss-Prot
Match: G3FIN8 (Linamarin synthase 1 OS=Manihot esculenta OX=3983 GN=UGT85K4 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.5e-60
Identity = 157/487 (32.24%), Postives = 234/487 (48.05%), Query Frame = 0

Query: 4   KKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGP 63
           +KP   I++P+P QGHV+PLM + K L  +G  IT +NT+  H +L+++ G      L P
Sbjct: 7   QKPPHAILVPYPAQGHVNPLMQLGKLLHARGFYITFVNTEHNHRRLIRSRGQEFIDGL-P 66

Query: 64  NIELTSLPDGFPPHEKRDGFR---QLFVELPINLKPQFETLLAKMNADEDA--ISCAIVD 123
           + +  ++PDG  P+  RD  +    L      +    F  L+AK+ A  D   I+C I D
Sbjct: 67  DFKFEAIPDGL-PYTDRDATQHVPSLSDSTRKHCLAPFIDLIAKLKASPDVPPITCIISD 126

Query: 124 MSMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKS-- 183
             MA+ ++ A   GI    +   +A   +      +L+  G+     VP K+   L    
Sbjct: 127 GVMAFAIDAARHFGILEIQFWTTSACGFMAYLHHIELVRRGI-----VPFKDESFLHDGT 186

Query: 184 -------LLGCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEP 243
                  + G P +  RD P   +   +   +MFD L         A   IL     LE 
Sbjct: 187 LDQPVDFIPGMPNMKLRDMP-SFIRVTDVNDIMFDFLGSEAHKSLKADAIILNTFDELEQ 246

Query: 244 QVVS-----LNPKLLTLGPLISTEAG-----------QLWKEDASCLEWLDRQSPGSVVY 303
           +V+       +  + T+GP I  E G            LWKED SCLEWLD++ P SVVY
Sbjct: 247 EVLDAIAARYSKNIYTVGPFILLEKGIPEIKSKAFRSSLWKEDLSCLEWLDKREPDSVVY 306

Query: 304 MAFGSTGDQTQQQFEELAMGMEMTRCPFLWVVR----AGTEKQFPKEFKGE---RGKIVS 363
           + +G     T +Q  E A G+  ++ PFLW+VR     G     P+EF  E   RG +VS
Sbjct: 307 VNYGCVTTITNEQLNEFAWGLANSKHPFLWIVRPDVVMGESAVLPEEFYEEIKDRGLLVS 366

Query: 364 WVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVG 423
           WVPQ +VL+HPAVG+FLSHCGWNS IE ++ G   +C P   +   N  Y  ++W+ GV 
Sbjct: 367 WVPQDRVLQHPAVGVFLSHCGWNSTIECISGGKPMICWPFFAEQQTNCKYACDVWKTGVE 426

Query: 424 LEKDHDGLVTRWEINRKVHQLMGDEAIKARSRAFSEEIGKCWEV--PHGRSVQNFDRLVR 452
           L  +    + R E+   + ++M  E  + R R   E   K  E     G S  NFD  ++
Sbjct: 427 LSTN----LKREELVSIIKEMMETEIGRERRRRAVEWRKKAEEAISVGGVSYNNFDTFIK 481

BLAST of Lcy09g003330 vs. ExPASy Swiss-Prot
Match: G3FIN9 (Linamarin synthase 2 OS=Manihot esculenta OX=3983 GN=UGT85K5 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.5e-59
Identity = 153/478 (32.01%), Postives = 229/478 (47.91%), Query Frame = 0

Query: 6   PARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGPNI 65
           P   +++P+P QGHV+PLM + K L  +G  IT +NT+  H +L+++ G      L P+ 
Sbjct: 9   PPHAVLVPYPAQGHVNPLMQLGKLLHSRGFYITFVNTEHNHRRLIRSRGQEFIDGL-PDF 68

Query: 66  ELTSLPDGFPPHEKRDGFR---QLFVELPINLKPQFETLLAKMNADEDA--ISCAIVDMS 125
           +  ++PDG  P+  RD  +    L      +    F  L+AK+ A  D   I+C I D  
Sbjct: 69  KFEAIPDGL-PYTDRDATQHVPSLSDSTRKHCLAPFIDLIAKLKASPDVPPITCIISDGV 128

Query: 126 MAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKS---- 185
           MA+ ++ A   GI    +   +A   +      +L+  G+     VP K+   L      
Sbjct: 129 MAFAIDAARHFGIPEIQFWTTSACGFMAYLHHIELVRRGI-----VPFKDESFLHDGTLD 188

Query: 186 -----LLGCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQV 245
                + G P +  RD P   +   +   +MFD +         A   IL     LE +V
Sbjct: 189 QPVDFIPGMPNMKLRDMP-SFIRVTDVNDIMFDFMGSEAHKSLKADAIILNTYDELEQEV 248

Query: 246 VS-----LNPKLLTLGPLISTEAG-----------QLWKEDASCLEWLDRQSPGSVVYMA 305
           +       +  + T+GP I  E G            LWKED SC+EWLD++ P SVVY+ 
Sbjct: 249 LDAIAARYSKNIYTVGPFILLEKGIPEIKSKAFRSSLWKEDLSCIEWLDKREPDSVVYVN 308

Query: 306 FGSTGDQTQQQFEELAMGMEMTRCPFLWVVR----AGTEKQFPKEFK---GERGKIVSWV 365
           +G     T +Q  E A G+  ++ PFLW+VR     G     P+EF     +RG +VSWV
Sbjct: 309 YGCVTTITNEQLNEFAWGLANSKHPFLWIVRPDVVMGESAVLPEEFYEAIKDRGLLVSWV 368

Query: 366 PQQKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLE 425
           PQ +VL+HPAVG+FLSHCGWNS IE ++ G   +C P   +   N  Y  ++W+ GV L 
Sbjct: 369 PQDRVLQHPAVGVFLSHCGWNSTIECISGGKPMICWPFFAEQQTNCKYACDVWKTGVELS 428

Query: 426 KDHDGLVTRWEINRKVHQLMGDEAIKARSRAFSEEIGKCWEVPH--GRSVQNFDRLVR 445
            +    + R E+   + ++M  E  + R R   E   K  E     G S  NFDR ++
Sbjct: 429 TN----LKREELVSIIKEMMETEIGRERRRRAVEWRKKAEEATSVGGVSYNNFDRFIK 474

BLAST of Lcy09g003330 vs. ExPASy Swiss-Prot
Match: Q9LME8 (UDP-glycosyltransferase 85A7 OS=Arabidopsis thaliana OX=3702 GN=UGT85A7 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.1e-58
Identity = 149/479 (31.11%), Postives = 240/479 (50.10%), Query Frame = 0

Query: 4   KKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGP 63
           +KP  V+ +P+P QGH++P++ ++K L  +G  +T +NT + HN+L+++ G N      P
Sbjct: 10  QKP-HVVCVPYPAQGHINPMLKVAKLLYAKGFHVTFVNTLYNHNRLLRSRGPNALDGF-P 69

Query: 64  NIELTSLPDGFPPHEKRDGFR-----QLFVELPINLKPQFETLLAKMNADEDA--ISCAI 123
           +    S+PDG P   + DG R      + + +  N    F+ +L ++N  +D   +SC +
Sbjct: 70  SFRFESIPDGLP---ETDGDRTQHTPTVCMSIEKNCLAPFKEILRRINDKDDVPPVSCIV 129

Query: 124 VDMSMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGV--IDPEGVPLKENVE- 183
            D  M++ L+ AEELG+   I+   +A   +        IE G+     E    KE+++ 
Sbjct: 130 SDGVMSFTLDAAEELGVPEVIFWTNSACGFMTILHFYLFIEKGLSPFKDESYMSKEHLDT 189

Query: 184 -LKSLLGCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVV 243
            +  +     L  +D P   +   N   +M + L+R       A   IL     LE  V+
Sbjct: 190 VIDWIPSMKNLRLKDIP-SYIRTTNPDNIMLNFLIREVERSKRASAIILNTFDELEHDVI 249

Query: 244 ----SLNPKLLTLGPL---------ISTEAGQ----LWKEDASCLEWLDRQSPGSVVYMA 303
               S+ P + ++GPL          ++E GQ    LW+E+  CL+WLD ++P SV+++ 
Sbjct: 250 QSMQSILPPVYSIGPLHLLVKEEINEASEIGQMGLNLWREEMECLDWLDTKTPNSVLFVN 309

Query: 304 FGSTGDQTQQQFEELAMGMEMTRCPFLWVVRAG-----TEKQFPKEFKGE---RGKIVSW 363
           FG     + +Q EE A G+  +R  FLWV+R            P+EF  E   R  + SW
Sbjct: 310 FGCITVMSAKQLEEFAWGLAASRKEFLWVIRPNLVVGEAMVVLPQEFLAETIDRRMLASW 369

Query: 364 VPQQKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGL 423
            PQ+KVL HPA+G FL+HCGWNS +E +  GV  +C PC  +   N  +  + W VG+ +
Sbjct: 370 CPQEKVLSHPAIGGFLTHCGWNSTLESLAGGVPMICWPCFSEQPTNCKFCCDEWGVGIEI 429

Query: 424 EKDHDGLVTRWEINRKVHQLM-GDEAIKARSRA--FSEEIGKCWEVPHGRSVQNFDRLV 444
            KD    V R E+   V +LM G++  K R +A  +     +     HG SV N + L+
Sbjct: 430 GKD----VKREEVETVVRELMDGEKGKKLREKAEEWRRLAEEATRYKHGSSVMNLETLI 478

BLAST of Lcy09g003330 vs. ExPASy Swiss-Prot
Match: Q9LMF0 (UDP-glycosyltransferase 85A5 OS=Arabidopsis thaliana OX=3702 GN=UGT85A5 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 8.0e-58
Identity = 155/478 (32.43%), Postives = 235/478 (49.16%), Query Frame = 0

Query: 4   KKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGP 63
           +KP  V+ IP P QGH++P++ ++K L  +G  +T +NT++ HN+L+++ G N    L P
Sbjct: 10  QKP-HVVCIPFPAQGHINPMLKVAKLLYARGFHVTFVNTNYNHNRLIRSRGPNSLDGL-P 69

Query: 64  NIELTSLPDGFPPHEK--RDGFRQLFVELPINLKPQFETLLAKMNADEDA--ISCAIVDM 123
           +    S+PDG P   K        L      N    F+ LL ++N  +D   +SC + D 
Sbjct: 70  SFRFESIPDGLPEENKDVMQDVPTLCESTMKNCLAPFKELLRRINTTKDVPPVSCIVSDG 129

Query: 124 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKSLLG 183
            M++ L+ AEELG+   ++   +A   +      + IE G+      P+K+   L + + 
Sbjct: 130 VMSFTLDAAEELGVPDVLFWTPSACGFLAYLHFYRFIEKGL-----SPIKDESSLDTKIN 189

Query: 184 CPP----LYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVV--- 243
             P    L  +D P   +   N + +M +  V        A   IL     LE  VV   
Sbjct: 190 WIPSMKNLGLKDIP-SFIRATNTEDIMLNFFVHEADRAKRASAIILNTFDSLEHDVVRSI 249

Query: 244 -SLNPKLLTLGPL---------ISTEAGQ----LWKEDASCLEWLDRQSPGSVVYMAFGS 303
            S+ P++ T+GPL           ++ GQ    +W+E+  CL+WLD +SP SVVY+ FGS
Sbjct: 250 QSIIPQVYTIGPLHLFVNRDIDEESDIGQIGTNMWREEMECLDWLDTKSPNSVVYVNFGS 309

Query: 304 TGDQTQQQFEELAMGMEMTRCPFLWVVR----AGTEKQFPKEF---KGERGKIVSWVPQQ 363
               + +Q  E A G+  T+  FLWV+R    AG     P +F      R  + SW PQ+
Sbjct: 310 ITVMSAKQLVEFAWGLAATKKDFLWVIRPDLVAGDVPMLPPDFLIETANRRMLASWCPQE 369

Query: 364 KVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDH 423
           KVL HPAVG FL+H GWNS +E ++ GV  +C P   +   N  Y  + W VG+ +  D 
Sbjct: 370 KVLSHPAVGGFLTHSGWNSTLESLSGGVPMVCWPFFAEQQTNCKYCCDEWEVGMEIGGD- 429

Query: 424 DGLVTRWEINRKVHQLM-GDEAIKARSRA-----FSEEIGKCWEVPHGRSVQNFDRLV 444
              V R E+   V +LM GD+  K R +A      +EE  K     +G S  NF  +V
Sbjct: 430 ---VRREEVEELVRELMDGDKGKKMRQKAEEWQRLAEEATK---PIYGSSELNFQMVV 472

BLAST of Lcy09g003330 vs. ExPASy TrEMBL
Match: A0A6J1FFY3 (UDP-glycosyltransferase 83A1-like OS=Cucurbita moschata OX=3662 GN=LOC111445126 PE=4 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 5.2e-185
Identity = 306/453 (67.55%), Postives = 372/453 (82.12%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           M +KK  RV+VIP P QGHV+PLMHISK LAKQGI+ITMINTDFIH+KL++AMG NM QI
Sbjct: 1   MAEKKGGRVVVIPFPAQGHVNPLMHISKGLAKQGIKITMINTDFIHSKLLEAMGQNMDQI 60

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV++  NLKPQFE LLAKMN D D  ++CAIVDM
Sbjct: 61  LGPNVELLGLPDGFPLHENRDDIHKLFVDIMDNLKPQFEKLLAKMNTDHDGGLACAIVDM 120

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G+I PEG P++E  +EL+SL 
Sbjct: 121 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIITPEGTPVEEKTIELESLG 180

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWRCVP KN+QK++FD+LVR+QRGCHYAK+ IL++PHVLEPQVVSLNPKL
Sbjct: 181 TPSMIHTSDLPWRCVPLKNSQKILFDHLVRVQRGCHYAKWYILILPHVLEPQVVSLNPKL 240

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYMAFGS+ D  Q+QFEE+AMGME+T 
Sbjct: 241 LTIGPLIATEAGQLWKEDDSCIRWLDQQPAGSVVYMAFGSSTDFNQEQFEEIAMGMELTE 300

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PF+WVVR GTE Q P  FKG +GKIVSWV QQKVL HP+VGIF+SHCGWNSVIEGV+SG
Sbjct: 301 RPFMWVVRQGTEHQLPAGFKGGKGKIVSWVSQQKVLNHPSVGIFMSHCGWNSVIEGVSSG 360

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + FLC P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + GDE +K RSR
Sbjct: 361 LPFLCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVGGDEEMKDRSR 420

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
             +EEI +CWE     S  NFDRLV+W KQE++
Sbjct: 421 VLAEEIRRCWEGADAPSALNFDRLVKWAKQEDD 453

BLAST of Lcy09g003330 vs. ExPASy TrEMBL
Match: A0A6J1K0L2 (UDP-glycosyltransferase 83A1-like OS=Cucurbita maxima OX=3661 GN=LOC111489540 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.9e-180
Identity = 298/453 (65.78%), Postives = 370/453 (81.68%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           MV+KK  RV+VIP P QGHV+PLMHISK LAKQGI+ITMINT FIH+KL+ AMG NM QI
Sbjct: 37  MVEKKGGRVVVIPFPAQGHVNPLMHISKGLAKQGIKITMINTHFIHSKLLAAMGQNMDQI 96

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV+   NLKPQFE LLAKMN ++D  ++CAIVDM
Sbjct: 97  LGPNVELLGLPDGFPLHENRDDIHKLFVDTMNNLKPQFEKLLAKMNTEQDGGLACAIVDM 156

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G++ PEG+P++E  +EL+SL 
Sbjct: 157 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIMTPEGIPVEEKTIELESLG 216

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWR VP+KN+QK++FD+LVR+QRGCHYAK+ IL+ PHVLEPQVVSLNPKL
Sbjct: 217 TPSMIHTSDLPWRRVPFKNSQKILFDHLVRVQRGCHYAKWYILIAPHVLEPQVVSLNPKL 276

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYM FGS+   +++QFEE+AMGME+T 
Sbjct: 277 LTIGPLIATEAGQLWKEDDSCIHWLDQQPAGSVVYMGFGSSTAFSREQFEEIAMGMELTE 336

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PFLWVVR GTE Q P  FKG++GKIVSWV QQKVL HP+VG+F+SHCGWNSVIEGV+SG
Sbjct: 337 RPFLWVVRQGTEHQLPAGFKGKKGKIVSWVSQQKVLNHPSVGVFMSHCGWNSVIEGVSSG 396

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + F+C P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + G+E  K RSR
Sbjct: 397 LPFVCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVAGNEETKGRSR 456

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
           A +E+IG+CWE     S  NF RLVRW KQ ++
Sbjct: 457 ALAEDIGRCWEGADAPSALNFARLVRWAKQGDD 489

BLAST of Lcy09g003330 vs. ExPASy TrEMBL
Match: A0A6J1K2Q0 (UDP-glycosyltransferase 83A1-like OS=Cucurbita maxima OX=3661 GN=LOC111489539 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 8.5e-180
Identity = 297/453 (65.56%), Postives = 369/453 (81.46%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           M +KK  RV+VIP P QGHV+PLMHISK LAKQGI+ITMINT FIH+KL+ AMG NM QI
Sbjct: 37  MAEKKGGRVVVIPFPAQGHVNPLMHISKGLAKQGIKITMINTHFIHSKLLTAMGQNMDQI 96

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV+   NLKPQFE LLAKMN ++D  ++CAIVDM
Sbjct: 97  LGPNVELLGLPDGFPLHENRDDIHKLFVDTMNNLKPQFEKLLAKMNTEQDGGLACAIVDM 156

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G++ PEG+P++E  +EL+SL 
Sbjct: 157 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIMTPEGIPVEEKTIELESLG 216

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWR VP+KN+QK++FD+LVR+QRGCHYAK+ IL+ PHVLEPQVVSLNPKL
Sbjct: 217 TPSMIHTSDLPWRRVPFKNSQKILFDHLVRVQRGCHYAKWYILIAPHVLEPQVVSLNPKL 276

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYM FGS+   +++QFEE+AMGME+T 
Sbjct: 277 LTIGPLIATEAGQLWKEDDSCIHWLDQQPAGSVVYMGFGSSTAFSREQFEEIAMGMELTE 336

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PFLWVVR GTE Q P  FKG++GKIVSWV QQKVL HP+VG+F+SHCGWNSVIEGV+SG
Sbjct: 337 RPFLWVVRQGTEHQLPAGFKGKKGKIVSWVSQQKVLNHPSVGVFMSHCGWNSVIEGVSSG 396

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + F+C P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + G+E  K RSR
Sbjct: 397 LPFVCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVAGNEETKGRSR 456

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
           A +E+IG+CWE     S  NF RLVRW KQ ++
Sbjct: 457 ALAEDIGRCWEGADAPSALNFARLVRWAKQGDD 489

BLAST of Lcy09g003330 vs. ExPASy TrEMBL
Match: A0A6J1BVD1 (UDP-glycosyltransferase 83A1-like OS=Momordica charantia OX=3673 GN=LOC111006050 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.6e-149
Identity = 246/443 (55.53%), Postives = 337/443 (76.07%), Query Frame = 0

Query: 9   VIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGPNIELT 68
           V+VIP+P QGHV+PLMH+ +  AKQGI+ T++NT+F+H K+V+AMGD +  +LG  IEL 
Sbjct: 6   VVVIPYPSQGHVNPLMHLCQSFAKQGIKSTVVNTEFVHAKMVKAMGDKIESVLGSRIELV 65

Query: 69  SLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNAD-EDAISCAIVDMSMAWVLEI 128
           ++PDGF P E RD   +LFV++  NL+ +FE LL KMN++    I+C IVD+SMAW+LEI
Sbjct: 66  AVPDGFGPDESRDNIERLFVDIMNNLQFEFEKLLPKMNSEIAGGIACVIVDLSMAWILEI 125

Query: 129 AEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLK-ENVELKSLLGCPPLYTR 188
            E+LGIRG+I++PFTAAHSV+N SIN+LIE+G+ID EGVP++   ++LK   G P + TR
Sbjct: 126 TEKLGIRGAIFSPFTAAHSVLNISINKLIEDGIIDAEGVPMQGARIQLKP--GMPYMRTR 185

Query: 189 DFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKLLTLGPLIS 248
           D PW CVP K +Q+++F++LVRIQRG   +K+C+    + LEP+ VS +PK+L +GPL++
Sbjct: 186 DLPWMCVPIKKSQRILFEHLVRIQRGYELSKWCLFNSAYELEPESVSFHPKILPVGPLMA 245

Query: 249 TEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTRCPFLWVVR 308
           +EAGQ WKEDASCLEWLD+Q P SV+Y+AFGS  D +  QFEELAMG+E+T  PFLWVVR
Sbjct: 246 SEAGQFWKEDASCLEWLDQQPPSSVIYLAFGSFTDFSGAQFEELAMGLELTGRPFLWVVR 305

Query: 309 AGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPC 368
            GTE++ P  F+G + KI+ W PQQKVL+HP+VG FLSHCGWNS++EGV++G+ FLC P 
Sbjct: 306 PGTEQKLPAGFRGRKAKIIGWAPQQKVLRHPSVGCFLSHCGWNSILEGVSNGLPFLCWPY 365

Query: 369 VGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSRAFSEEIGK 428
           VGD H N  Y+ +IW+VG+G ++D  G V+RWEI +KV QL+GD + +ARS    + +  
Sbjct: 366 VGDQHFNMSYVDDIWKVGLGFDRDEKGTVSRWEIKKKVEQLLGDPSFRARSAQLQQLVRN 425

Query: 429 CWEVPHGRSVQNFDRLVRWIKQE 450
             + P G S  N D+ VRW+K+E
Sbjct: 426 SVK-PEGLSSINVDKFVRWVKEE 445

BLAST of Lcy09g003330 vs. ExPASy TrEMBL
Match: A0A067K4T2 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_19934 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 188/445 (42.25%), Postives = 283/445 (63.60%), Query Frame = 0

Query: 9   VIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGPNIELT 68
           V+VIP+P QGHV+PLMH S+ +A  GI+IT +NTDF+H +++ A+ DN      P + L 
Sbjct: 6   VLVIPYPAQGHVNPLMHFSQRIANLGIKITFVNTDFVHKRVLSAIVDNDSVGSSPFVNLV 65

Query: 69  SLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADEDAISCAIVDMSMAWVLEIA 128
           S+PDG    + R+ F +L   +   +  + E L+  +N D+  ISC + D  M WV E+A
Sbjct: 66  SIPDGLGSEDDRNDFGKLCDSMLTTMLKKLEELIQDLNGDDGPISCIVADGHMGWVREVA 125

Query: 129 EELGIRGSIYTPFTAAHSVINFSINQLIENGVID-PEGVPLKENVELKSLLGCPPLYTRD 188
           +++GIR +I  P +AA   +   I +LI +G ID  +G   K+++ ++   G P   T++
Sbjct: 126 KKIGIRVAIVWPASAALFSLEAIIPKLIRDGTIDSSDGFSTKKHI-IQLSPGIPNFDTQN 185

Query: 189 FPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKLLTLGPLIS- 248
            PW      N+QK +F+ + R+  G   A + +    + LEP+  SL P LL +GPLI+ 
Sbjct: 186 MPWNLGGDSNSQKAIFEYIQRMTEGSKMADWKLCNSTYDLEPEAFSLTPNLLPIGPLIAN 245

Query: 249 ----TEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTRCPFL 308
               +   QLW+ED+SCL+WLD+Q P SV+Y+AFGST    Q QF+ELA+G+++T  PFL
Sbjct: 246 YNTGSSGSQLWQEDSSCLKWLDQQKPQSVIYVAFGSTTIFNQTQFQELALGLQLTEKPFL 305

Query: 309 WVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFL 368
           WVVR G  +++P EF+G  GKIVSWVPQQKVL HP++  F+SHCGWNS IEGV++GV FL
Sbjct: 306 WVVRPGITEEYPDEFQGRNGKIVSWVPQQKVLSHPSIACFVSHCGWNSTIEGVSNGVPFL 365

Query: 369 CMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSRAFSE 428
           C P   D  LN+ Y+ EIW+VG+G +KD +G++ + EI  K+ +L  D++I+ RS    +
Sbjct: 366 CWPYFADQFLNESYICEIWKVGLGFDKDENGIIRKGEIKEKMDRLFSDKSIRERSLNLKQ 425

Query: 429 EIGKCWEVPHGRSVQNFDRLVRWIK 448
            + +      G+S  NF   ++W++
Sbjct: 426 RV-RSSVGEGGQSSANFTNFIKWLE 448

BLAST of Lcy09g003330 vs. NCBI nr
Match: KAG6578540.1 (UDP-glycosyltransferase 83A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 657.9 bits (1696), Expect = 6.3e-185
Identity = 305/453 (67.33%), Postives = 373/453 (82.34%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           M +KK  RV+VIP P QGHV+PLMHISK LAK+GI+ITMINTDFIH+KL++AMG N+ QI
Sbjct: 1   MAEKKGGRVVVIPFPAQGHVNPLMHISKGLAKEGIKITMINTDFIHSKLLEAMGQNIDQI 60

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV++  NLKPQFE LLAKMN D D  ++CAIVDM
Sbjct: 61  LGPNVELLGLPDGFPLHENRDDIHKLFVDIMNNLKPQFEKLLAKMNTDHDGGLACAIVDM 120

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G+I PEG P++E  +EL+SL 
Sbjct: 121 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIITPEGTPVEEKTIELESLG 180

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWRCVP KN+QK++FD+LVR+QRGCHYAK+ IL++PHVLEPQVVSLNPKL
Sbjct: 181 TPSMIHTSDLPWRCVPLKNSQKILFDHLVRVQRGCHYAKWYILILPHVLEPQVVSLNPKL 240

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYMAFGS+ D  Q+QFEE+AMGME+T 
Sbjct: 241 LTIGPLIATEAGQLWKEDDSCIRWLDQQPAGSVVYMAFGSSTDFNQEQFEEIAMGMELTE 300

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PF+WVVR GTE Q P  FKGE+GKIVSWV QQKVL HP+VGIF+SHCGWNSVIEGV+SG
Sbjct: 301 RPFMWVVRQGTEHQLPAGFKGEKGKIVSWVSQQKVLNHPSVGIFMSHCGWNSVIEGVSSG 360

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + FLC P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + GDE +K RSR
Sbjct: 361 LPFLCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVGGDEEMKGRSR 420

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
             +EEI +CWE     S  NFDRLV+W KQE++
Sbjct: 421 VLAEEIRRCWEGADAPSALNFDRLVKWAKQEDD 453

BLAST of Lcy09g003330 vs. NCBI nr
Match: XP_022939127.1 (UDP-glycosyltransferase 83A1-like [Cucurbita moschata])

HSP 1 Score: 657.1 bits (1694), Expect = 1.1e-184
Identity = 306/453 (67.55%), Postives = 372/453 (82.12%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           M +KK  RV+VIP P QGHV+PLMHISK LAKQGI+ITMINTDFIH+KL++AMG NM QI
Sbjct: 1   MAEKKGGRVVVIPFPAQGHVNPLMHISKGLAKQGIKITMINTDFIHSKLLEAMGQNMDQI 60

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV++  NLKPQFE LLAKMN D D  ++CAIVDM
Sbjct: 61  LGPNVELLGLPDGFPLHENRDDIHKLFVDIMDNLKPQFEKLLAKMNTDHDGGLACAIVDM 120

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G+I PEG P++E  +EL+SL 
Sbjct: 121 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIITPEGTPVEEKTIELESLG 180

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWRCVP KN+QK++FD+LVR+QRGCHYAK+ IL++PHVLEPQVVSLNPKL
Sbjct: 181 TPSMIHTSDLPWRCVPLKNSQKILFDHLVRVQRGCHYAKWYILILPHVLEPQVVSLNPKL 240

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYMAFGS+ D  Q+QFEE+AMGME+T 
Sbjct: 241 LTIGPLIATEAGQLWKEDDSCIRWLDQQPAGSVVYMAFGSSTDFNQEQFEEIAMGMELTE 300

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PF+WVVR GTE Q P  FKG +GKIVSWV QQKVL HP+VGIF+SHCGWNSVIEGV+SG
Sbjct: 301 RPFMWVVRQGTEHQLPAGFKGGKGKIVSWVSQQKVLNHPSVGIFMSHCGWNSVIEGVSSG 360

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + FLC P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + GDE +K RSR
Sbjct: 361 LPFLCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVGGDEEMKDRSR 420

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
             +EEI +CWE     S  NFDRLV+W KQE++
Sbjct: 421 VLAEEIRRCWEGADAPSALNFDRLVKWAKQEDD 453

BLAST of Lcy09g003330 vs. NCBI nr
Match: XP_023549730.1 (UDP-glycosyltransferase 83A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656.4 bits (1692), Expect = 1.8e-184
Identity = 305/453 (67.33%), Postives = 373/453 (82.34%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           M ++K  RV+VIP P QGHV+PLMHISK LAKQGI+ITMINTDFIH+KL++AMG NM QI
Sbjct: 1   MAERKGERVVVIPFPAQGHVNPLMHISKGLAKQGIKITMINTDFIHSKLLEAMGQNMDQI 60

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  L DGFP HE RD   +LFV++  NLKPQFE LLAKMN D D  ++CAIVDM
Sbjct: 61  LGPNVELLGLSDGFPLHENRDDIHKLFVDIMNNLKPQFEKLLAKMNTDHDGGLACAIVDM 120

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G+I PEG+P++E  +EL+SL 
Sbjct: 121 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIITPEGIPVEEKTIELESLG 180

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWRCVP+KN+QK++FD+LVR+QRGCHYAK+ IL+VPHVLEPQVVSLNPKL
Sbjct: 181 TPSMIHTSDLPWRCVPFKNSQKILFDHLVRVQRGCHYAKWYILIVPHVLEPQVVSLNPKL 240

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYMAFGS+ D  Q+QFEE+AMGME+T 
Sbjct: 241 LTIGPLIATEAGQLWKEDESCIHWLDQQPAGSVVYMAFGSSTDFNQEQFEEIAMGMELTE 300

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PF+WVVR GTE Q P  FKG +GKIVSWV QQKVL HP+VGIF+SHCGW+SVIEGV+SG
Sbjct: 301 RPFMWVVRQGTEHQLPAGFKGGKGKIVSWVSQQKVLNHPSVGIFMSHCGWDSVIEGVSSG 360

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + FLC P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + GDE +K RSR
Sbjct: 361 LPFLCWPYVGDHFLNMKYVTEIWKVGIGFERELNGVVSRWEIKKKVELVGGDEEMKGRSR 420

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
             +EEI +CWE     S  NFDRLVRW KQE++
Sbjct: 421 VLAEEIRRCWEGADAPSALNFDRLVRWAKQEDD 453

BLAST of Lcy09g003330 vs. NCBI nr
Match: KAG7016100.1 (UDP-glycosyltransferase 83A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 654.4 bits (1687), Expect = 6.9e-184
Identity = 304/453 (67.11%), Postives = 372/453 (82.12%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           M +KK  RV+VIP P QGHV+PLMHISK LAK+GI+ITMINTDFIH+KL++AMG N+ QI
Sbjct: 1   MAEKKGGRVVVIPFPAQGHVNPLMHISKGLAKEGIKITMINTDFIHSKLLEAMGQNIDQI 60

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV++  NLKPQFE LLAKMN D D  ++CAIVDM
Sbjct: 61  LGPNVELLGLPDGFPLHENRDDIHKLFVDIMNNLKPQFEKLLAKMNTDHDGGLACAIVDM 120

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G+I PEG P++E  +EL+SL 
Sbjct: 121 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIITPEGTPVEEKTIELESLG 180

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWRCVP KN+QK++FD+LVR+QRGCHYAK+ IL++PHVLEPQVVSLNPKL
Sbjct: 181 TPSMIHTSDLPWRCVPLKNSQKILFDHLVRVQRGCHYAKWYILILPHVLEPQVVSLNPKL 240

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYMAFGS+ D  Q+QFEE+AMGME+T 
Sbjct: 241 LTIGPLIATEAGQLWKEDDSCIRWLDQQPAGSVVYMAFGSSTDFNQEQFEEIAMGMELTE 300

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PF+WVVR GTE Q P  FKG +GKIVSWV QQKVL HP+VGIF+SHCGWNSVIEGV+SG
Sbjct: 301 RPFMWVVRQGTEHQLPAGFKGGKGKIVSWVSQQKVLNHPSVGIFMSHCGWNSVIEGVSSG 360

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + FLC P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + GDE +K RSR
Sbjct: 361 LPFLCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVGGDEEMKDRSR 420

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
             +EEI +CWE     S  NFDRLV+W KQE++
Sbjct: 421 VLAEEIRRCWEGADAPSALNFDRLVKWAKQEDD 453

BLAST of Lcy09g003330 vs. NCBI nr
Match: XP_022993589.1 (UDP-glycosyltransferase 83A1-like [Cucurbita maxima])

HSP 1 Score: 641.3 bits (1653), Expect = 6.1e-180
Identity = 298/453 (65.78%), Postives = 370/453 (81.68%), Query Frame = 0

Query: 1   MVDKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQI 60
           MV+KK  RV+VIP P QGHV+PLMHISK LAKQGI+ITMINT FIH+KL+ AMG NM QI
Sbjct: 37  MVEKKGGRVVVIPFPAQGHVNPLMHISKGLAKQGIKITMINTHFIHSKLLAAMGQNMDQI 96

Query: 61  LGPNIELTSLPDGFPPHEKRDGFRQLFVELPINLKPQFETLLAKMNADED-AISCAIVDM 120
           LGPN+EL  LPDGFP HE RD   +LFV+   NLKPQFE LLAKMN ++D  ++CAIVDM
Sbjct: 97  LGPNVELLGLPDGFPLHENRDDIHKLFVDTMNNLKPQFEKLLAKMNTEQDGGLACAIVDM 156

Query: 121 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKE-NVELKSLL 180
           SM WVLEIA  LG++G+I+TPFTAAHSV+N S+N+LIE+G++ PEG+P++E  +EL+SL 
Sbjct: 157 SMGWVLEIAASLGVKGAIFTPFTAAHSVLNLSLNELIESGIMTPEGIPVEEKTIELESLG 216

Query: 181 GCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKL 240
               ++T D PWR VP+KN+QK++FD+LVR+QRGCHYAK+ IL+ PHVLEPQVVSLNPKL
Sbjct: 217 TPSMIHTSDLPWRRVPFKNSQKILFDHLVRVQRGCHYAKWYILIAPHVLEPQVVSLNPKL 276

Query: 241 LTLGPLISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGMEMTR 300
           LT+GPLI+TEAGQLWKED SC+ WLD+Q  GSVVYM FGS+   +++QFEE+AMGME+T 
Sbjct: 277 LTIGPLIATEAGQLWKEDDSCIHWLDQQPAGSVVYMGFGSSTAFSREQFEEIAMGMELTE 336

Query: 301 CPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGVTSG 360
            PFLWVVR GTE Q P  FKG++GKIVSWV QQKVL HP+VG+F+SHCGWNSVIEGV+SG
Sbjct: 337 RPFLWVVRQGTEHQLPAGFKGKKGKIVSWVSQQKVLNHPSVGVFMSHCGWNSVIEGVSSG 396

Query: 361 VSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKARSR 420
           + F+C P VGDH LN  Y+TEIW+VG+G E++ +G+V+RWEI +KV  + G+E  K RSR
Sbjct: 397 LPFVCWPYVGDHFLNMKYVTEIWKVGIGFEREVNGVVSRWEIKKKVELVAGNEETKGRSR 456

Query: 421 AFSEEIGKCWEVPHGRSVQNFDRLVRWIKQEEE 452
           A +E+IG+CWE     S  NF RLVRW KQ ++
Sbjct: 457 ALAEDIGRCWEGADAPSALNFARLVRWAKQGDD 489

BLAST of Lcy09g003330 vs. TAIR 10
Match: AT3G02100.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 9.6e-91
Identity = 169/454 (37.22%), Postives = 259/454 (57.05%), Query Frame = 0

Query: 9   VIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQ-ILGPNIEL 68
           V+VIP+P QGHV PL+  S++LAKQGI+IT INT+F HN+++ ++ ++ H+  +G  I L
Sbjct: 14  VVVIPYPAQGHVLPLISFSRYLAKQGIQITFINTEFNHNRIISSLPNSPHEDYVGDQINL 73

Query: 69  TSLPDGFPPHEKRDGFRQLFVELPINLKP-QFETLLAKMNADEDA---ISCAIVDMSMAW 128
            S+PDG     +         E  +   P + E L+ +M A+      ISC + D S+ W
Sbjct: 74  VSIPDGLEDSPEERNIPGKLSESVLRFMPKKVEELIERMMAETSGGTIISCVVADQSLGW 133

Query: 129 VLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKSLLGCPPL 188
            +E+A + GIR + + P  AA  V+ FSI +LI++G+ID +G  ++ N  ++   G P +
Sbjct: 134 AIEVAAKFGIRRTAFCPAAAASMVLGFSIQKLIDDGLIDSDGT-VRVNKTIQLSPGMPKM 193

Query: 189 YTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVVSLNPKLLTLGP 248
            T  F W C+  K +QK +F  +++         + +    H LE     L P ++ +GP
Sbjct: 194 ETDKFVWVCLKNKESQKNIFQLMLQNNNSIESTDWLLCNSVHELETAAFGLGPNIVPIGP 253

Query: 249 L--------ISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQTQQQFEELAMGME 308
           +         ST  G     D  CL+WLDRQ PGSV+Y+AFGS G     Q EELA+G+E
Sbjct: 254 IGWAHSLEEGSTSLGSFLPHDRDCLDWLDRQIPGSVIYVAFGSFGVMGNPQLEELAIGLE 313

Query: 309 MTRCPFLWVVRAGTEKQFPKEFKGERGKIVSWVPQQKVLKHPAVGIFLSHCGWNSVIEGV 368
           +T+ P LWV    T  Q P +   +R K+V W PQ++VL   A+G F+SHCGWNS +EG 
Sbjct: 314 LTKRPVLWV----TGDQQPIKLGSDRVKVVRWAPQREVLSSGAIGCFVSHCGWNSTLEGA 373

Query: 369 TSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVTRWEINRKVHQLMGDEAIKA 428
            +G+ FLC+P   D  +NK Y+ ++W++G+GLE+D  G+V R E+ +K+ ++M D     
Sbjct: 374 QNGIPFLCIPYFADQFINKAYICDVWKIGLGLERDARGVVPRLEVKKKIDEIMRDGGEYE 433

Query: 429 RSRAFSEEIGKCWEVPHGRSVQNFDRLVRWIKQE 450
                 +EI        G S +N ++ V WIK +
Sbjct: 434 ERAMKVKEIVMKSVAKDGISCENLNKFVNWIKSQ 462

BLAST of Lcy09g003330 vs. TAIR 10
Match: AT1G22340.1 (UDP-glucosyl transferase 85A7 )

HSP 1 Score: 226.5 bits (576), Expect = 4.3e-59
Identity = 149/479 (31.11%), Postives = 240/479 (50.10%), Query Frame = 0

Query: 4   KKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGP 63
           +KP  V+ +P+P QGH++P++ ++K L  +G  +T +NT + HN+L+++ G N      P
Sbjct: 10  QKP-HVVCVPYPAQGHINPMLKVAKLLYAKGFHVTFVNTLYNHNRLLRSRGPNALDGF-P 69

Query: 64  NIELTSLPDGFPPHEKRDGFR-----QLFVELPINLKPQFETLLAKMNADEDA--ISCAI 123
           +    S+PDG P   + DG R      + + +  N    F+ +L ++N  +D   +SC +
Sbjct: 70  SFRFESIPDGLP---ETDGDRTQHTPTVCMSIEKNCLAPFKEILRRINDKDDVPPVSCIV 129

Query: 124 VDMSMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGV--IDPEGVPLKENVE- 183
            D  M++ L+ AEELG+   I+   +A   +        IE G+     E    KE+++ 
Sbjct: 130 SDGVMSFTLDAAEELGVPEVIFWTNSACGFMTILHFYLFIEKGLSPFKDESYMSKEHLDT 189

Query: 184 -LKSLLGCPPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVV 243
            +  +     L  +D P   +   N   +M + L+R       A   IL     LE  V+
Sbjct: 190 VIDWIPSMKNLRLKDIP-SYIRTTNPDNIMLNFLIREVERSKRASAIILNTFDELEHDVI 249

Query: 244 ----SLNPKLLTLGPL---------ISTEAGQ----LWKEDASCLEWLDRQSPGSVVYMA 303
               S+ P + ++GPL          ++E GQ    LW+E+  CL+WLD ++P SV+++ 
Sbjct: 250 QSMQSILPPVYSIGPLHLLVKEEINEASEIGQMGLNLWREEMECLDWLDTKTPNSVLFVN 309

Query: 304 FGSTGDQTQQQFEELAMGMEMTRCPFLWVVRAG-----TEKQFPKEFKGE---RGKIVSW 363
           FG     + +Q EE A G+  +R  FLWV+R            P+EF  E   R  + SW
Sbjct: 310 FGCITVMSAKQLEEFAWGLAASRKEFLWVIRPNLVVGEAMVVLPQEFLAETIDRRMLASW 369

Query: 364 VPQQKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGL 423
            PQ+KVL HPA+G FL+HCGWNS +E +  GV  +C PC  +   N  +  + W VG+ +
Sbjct: 370 CPQEKVLSHPAIGGFLTHCGWNSTLESLAGGVPMICWPCFSEQPTNCKFCCDEWGVGIEI 429

Query: 424 EKDHDGLVTRWEINRKVHQLM-GDEAIKARSRA--FSEEIGKCWEVPHGRSVQNFDRLV 444
            KD    V R E+   V +LM G++  K R +A  +     +     HG SV N + L+
Sbjct: 430 GKD----VKREEVETVVRELMDGEKGKKLREKAEEWRRLAEEATRYKHGSSVMNLETLI 478

BLAST of Lcy09g003330 vs. TAIR 10
Match: AT1G22370.2 (UDP-glucosyl transferase 85A5 )

HSP 1 Score: 226.1 bits (575), Expect = 5.7e-59
Identity = 155/478 (32.43%), Postives = 235/478 (49.16%), Query Frame = 0

Query: 4   KKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGP 63
           +KP  V+ IP P QGH++P++ ++K L  +G  +T +NT++ HN+L+++ G N    L P
Sbjct: 10  QKP-HVVCIPFPAQGHINPMLKVAKLLYARGFHVTFVNTNYNHNRLIRSRGPNSLDGL-P 69

Query: 64  NIELTSLPDGFPPHEK--RDGFRQLFVELPINLKPQFETLLAKMNADEDA--ISCAIVDM 123
           +    S+PDG P   K        L      N    F+ LL ++N  +D   +SC + D 
Sbjct: 70  SFRFESIPDGLPEENKDVMQDVPTLCESTMKNCLAPFKELLRRINTTKDVPPVSCIVSDG 129

Query: 124 SMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKSLLG 183
            M++ L+ AEELG+   ++   +A   +      + IE G+      P+K+   L + + 
Sbjct: 130 VMSFTLDAAEELGVPDVLFWTPSACGFLAYLHFYRFIEKGL-----SPIKDESSLDTKIN 189

Query: 184 CPP----LYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVV--- 243
             P    L  +D P   +   N + +M +  V        A   IL     LE  VV   
Sbjct: 190 WIPSMKNLGLKDIP-SFIRATNTEDIMLNFFVHEADRAKRASAIILNTFDSLEHDVVRSI 249

Query: 244 -SLNPKLLTLGPL---------ISTEAGQ----LWKEDASCLEWLDRQSPGSVVYMAFGS 303
            S+ P++ T+GPL           ++ GQ    +W+E+  CL+WLD +SP SVVY+ FGS
Sbjct: 250 QSIIPQVYTIGPLHLFVNRDIDEESDIGQIGTNMWREEMECLDWLDTKSPNSVVYVNFGS 309

Query: 304 TGDQTQQQFEELAMGMEMTRCPFLWVVR----AGTEKQFPKEF---KGERGKIVSWVPQQ 363
               + +Q  E A G+  T+  FLWV+R    AG     P +F      R  + SW PQ+
Sbjct: 310 ITVMSAKQLVEFAWGLAATKKDFLWVIRPDLVAGDVPMLPPDFLIETANRRMLASWCPQE 369

Query: 364 KVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDH 423
           KVL HPAVG FL+H GWNS +E ++ GV  +C P   +   N  Y  + W VG+ +  D 
Sbjct: 370 KVLSHPAVGGFLTHSGWNSTLESLSGGVPMVCWPFFAEQQTNCKYCCDEWEVGMEIGGD- 429

Query: 424 DGLVTRWEINRKVHQLM-GDEAIKARSRA-----FSEEIGKCWEVPHGRSVQNFDRLV 444
              V R E+   V +LM GD+  K R +A      +EE  K     +G S  NF  +V
Sbjct: 430 ---VRREEVEELVRELMDGDKGKKMRQKAEEWQRLAEEATK---PIYGSSELNFQMVV 472

BLAST of Lcy09g003330 vs. TAIR 10
Match: AT1G22380.1 (UDP-glucosyl transferase 85A3 )

HSP 1 Score: 225.7 bits (574), Expect = 7.4e-59
Identity = 153/476 (32.14%), Postives = 232/476 (48.74%), Query Frame = 0

Query: 3   DKKPARVIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILG 62
           ++KP  V+ +P+P QGH++P+M ++K L  +G  +T +NT + HN+L+++ G N    L 
Sbjct: 9   EQKP-HVVCVPYPAQGHINPMMKVAKLLHVKGFHVTFVNTVYNHNRLLRSRGANALDGL- 68

Query: 63  PNIELTSLPDGFPPH--EKRDGFRQLFVELPINLKPQFETLLAKMNADEDA--ISCAIVD 122
           P+ +  S+PDG P    +       L      N    F+ LL ++   ED   +SC + D
Sbjct: 69  PSFQFESIPDGLPETGVDATQDIPALSESTTKNCLVPFKKLLQRIVTREDVPPVSCIVSD 128

Query: 123 MSMAWVLEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGVIDPEGVPLKENVELKSLL 182
            SM++ L++AEELG+    +   +A   +        IE G+   +         L +++
Sbjct: 129 GSMSFTLDVAEELGVPEIHFWTTSACGFMAYLHFYLFIEKGLCPVKDASCLTKEYLDTVI 188

Query: 183 GCPP----LYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVV-- 242
              P    +  +D P   +   N   +M + +VR       A   IL     LE  ++  
Sbjct: 189 DWIPSMNNVKLKDIP-SFIRTTNPNDIMLNFVVREACRTKRASAIILNTFDDLEHDIIQS 248

Query: 243 --SLNPKLLTLGPL-------------ISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFG 302
             S+ P +  +GPL             I      LWKE+  CL WL+ +S  SVVY+ FG
Sbjct: 249 MQSILPPVYPIGPLHLLVNREIEEDSEIGRMGSNLWKEETECLGWLNTKSRNSVVYVNFG 308

Query: 303 STGDQTQQQFEELAMGMEMTRCPFLWVVR----AGTEKQFPKEFKGE---RGKIVSWVPQ 362
           S    T  Q  E A G+  T   FLWV+R    AG E   PKEF  E   R  + SW PQ
Sbjct: 309 SITIMTTAQLLEFAWGLAATGKEFLWVMRPDSVAGEEAVIPKEFLAETADRRMLTSWCPQ 368

Query: 363 QKVLKHPAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKD 422
           +KVL HPAVG FL+HCGWNS +E ++ GV  +C P   +   N  +  + W VG+ +  D
Sbjct: 369 EKVLSHPAVGGFLTHCGWNSTLESLSCGVPMVCWPFFAEQQTNCKFSCDEWEVGIEIGGD 428

Query: 423 HDGLVTRWEINRKVHQLM-GDEAIKARSRA--FSEEIGKCWEVPHGRSVQNFDRLV 444
               V R E+   V +LM G++  K R +A  +     K  ++P G SV NF+ +V
Sbjct: 429 ----VKRGEVEAVVRELMDGEKGKKMREKAVEWRRLAEKATKLPCGSSVINFETIV 477

BLAST of Lcy09g003330 vs. TAIR 10
Match: AT1G22360.1 (UDP-glucosyl transferase 85A2 )

HSP 1 Score: 224.6 bits (571), Expect = 1.6e-58
Identity = 146/470 (31.06%), Postives = 230/470 (48.94%), Query Frame = 0

Query: 9   VIVIPHPLQGHVHPLMHISKHLAKQGIRITMINTDFIHNKLVQAMGDNMHQILGPNIELT 68
           V+ +P+P QGH++P+M ++K L  +G  IT +NT + HN+L+++ G N    L P+    
Sbjct: 11  VVCVPYPAQGHINPMMKVAKLLYAKGFHITFVNTVYNHNRLLRSRGPNAVDGL-PSFRFE 70

Query: 69  SLPDGFPPH--EKRDGFRQLFVELPINLKPQFETLLAKMNADEDA--ISCAIVDMSMAWV 128
           S+PDG P    +       L      +    F+ LL ++NA +D   +SC + D  M++ 
Sbjct: 71  SIPDGLPETDVDVTQDIPTLCESTMKHCLAPFKELLRQINARDDVPPVSCIVSDGCMSFT 130

Query: 129 LEIAEELGIRGSIYTPFTAAHSVINFSINQLIENGV--IDPEGVPLKENVELKS--LLGC 188
           L+ AEELG+   ++   +A   +      + IE G+  I  E    KE+++ K   +   
Sbjct: 131 LDAAEELGVPEVLFWTTSACGFLAYLYYYRFIEKGLSPIKDESYLTKEHLDTKIDWIPSM 190

Query: 189 PPLYTRDFPWRCVPWKNAQKMMFDNLVRIQRGCHYAKFCILVVPHVLEPQVV----SLNP 248
             L  +D P   +   N   +M + ++R       A   IL     LE  V+    S+ P
Sbjct: 191 KNLRLKDIP-SFIRTTNPDDIMLNFIIREADRAKRASAIILNTFDDLEHDVIQSMKSIVP 250

Query: 249 KLLTLGPL-------------ISTEAGQLWKEDASCLEWLDRQSPGSVVYMAFGSTGDQT 308
            + ++GPL             I      LW+E+  CL+WL+ ++  SVVY+ FGS    +
Sbjct: 251 PVYSIGPLHLLEKQESGEYSEIGRTGSNLWREETECLDWLNTKARNSVVYVNFGSITVLS 310

Query: 309 QQQFEELAMGMEMTRCPFLWVVR----AGTEKQFPKEF---KGERGKIVSWVPQQKVLKH 368
            +Q  E A G+  T   FLWV+R    AG E   P EF     +R  + SW PQ+KVL H
Sbjct: 311 AKQLVEFAWGLAATGKEFLWVIRPDLVAGDEAMVPPEFLTATADRRMLASWCPQEKVLSH 370

Query: 369 PAVGIFLSHCGWNSVIEGVTSGVSFLCMPCVGDHHLNKHYLTEIWRVGVGLEKDHDGLVT 428
           PA+G FL+HCGWNS +E +  GV  +C P   +   N  +  + W VG+ +  D    V 
Sbjct: 371 PAIGGFLTHCGWNSTLESLCGGVPMVCWPFFAEQQTNCKFSRDEWEVGIEIGGD----VK 430

Query: 429 RWEINRKVHQLMGDEA---IKARSRAFSEEIGKCWEVPHGRSVQNFDRLV 444
           R E+   V +LM +E    ++ ++  +     +  E  HG S  NF+ LV
Sbjct: 431 REEVEAVVRELMDEEKGKNMREKAEEWRRLANEATEHKHGSSKLNFEMLV 474