Lcy09g003190 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g003190
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Cytochrome P450
Location	Chr09: 2719537 .. 2722562 (-)
RNA-Seq Expression	Lcy09g003190
Synteny	Lcy09g003190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAACAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGAGCTTCTTGGGACCTTCAATGGCGCTGGCCTTAGCCATTCTTGCTCTGTTCTTGCTCCTCTGTTTTTGCTCTGCTTTACTGCGATGGAATGAAGTTCGATACAGTAAAAAGGGGCTGCCTCCTGGTACAATGGGCTGGCCTGTTTTTGGTGAAACCACTGAGTTTCTCAAACAAGGTCCTAACTTTATGAAATCCCAGAGAGCAAGGTAACTATTTCTCTGTTTCTTTGCTCTGTTTTAGCCCTTTGGATTTGTGGGCTTTTAACCATTGCTCTGTTTTTCGTTCTTGAAAAGGTATGGAAGCATTTTCAAGTCCCATATTTTGGGCTGTCCTACCATTGTTTCTATGGATCCTGAGGTTAATAGATATGTTTTAATGAATGAATCAAAAGGGCTTGTCCCTGGCTACCCTCAGTCCATGCTTGATATCCTTGGCAAATGCAATATCGCCGCCGTCCACGGCGGCGCTCACAAGCTGATGAGAGCTGCTCTGCTCGCCGTTGTCAGCCCCACCATGATTAAGGACCGTCTTCTTCCTAAAGTTGATGAATTTATGCGATCCCATTTGAGGAATTGGGATCAACAAATCATTGACATCCAGGACAAGACTAAAGAGGTGAACTTTATTTTAAAAAATGCCGTTTTGTTTTGATGGTGTAGTTCTCCTTCCTCTGTTTGTACTCATGTTTTTCTCCTCTGTTTTTGTCTTCAGATGGCTCTCCGGTCGTCTTTGAAGCAGATTGCCGGGATCGAGTCCGGTCCGTTATCGGAATCTTTCATCCCGGAGTTCTTTAAGCTTGTTCTCGGCACCCTCTCGCTCCCCATCGATCTCCCCGGCACCAACTACCGCCGAGGAATTCAGGTAAACTTAAGTTTCTAAAATTAACATGGTATCAAAGCAGGATGTCTTGTGTTCGAATTTCGATAATGTCAATCCTATCACTTTTTGGGCTTTCTGTCCATTTTCGAGCCTAAAAAGATGAATGAAAAGGAGAGTTGTGATTCTCAGGCGAGGAAGAACATAATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAGAGACAAAGAAAGATGACATGCTTGGATATTTCATGAGTGAAGAGAACAAATACAAGCTAAACGATGAAGAGATTATCGATCAGATCATAACAGTTCTGTACTCTGGCTATGAAACTGTTTCGACAACTTCTATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAAATCAGAGTGAGTTTTTAAAACAAGAAACGAAAAAGATTTACAACTTTTCTGAGTTGGAACTTTAAAAAGGCTGTAGGGGGGTTTTTGGTTTCTTTTTTCTTTTTCTAAGTTAAGAGATTTGAAAACTGCAGGAAGAGCATTTGGCAATCAGAGAAAAGAAAAATCCTGAGGACCCCATTGATTGGGATGATCTTAAAGCCATGAAGTTCACTCGTGCGGTAGGATTTTTATGTTTTTACTGTTGTCTTTTTAACAGTTCTTTGATGGGTTTTTTTTTTTTAAGGAAAAAAAGACTCTAACTTTTACTCTGTTTTGAATTGTTTAGGTGATATTTGAAACATCAAGGCTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACAAAGGACATGGAACTAAATGGTGAGTTTCATAAAGAAAAAAAAAATCATTATAAAGAATAAAACTTGGTTGCTGACAAGCATTTGGGGCAGGGCAAGTTTTTTTTTTTTTTTTTTCCTTTTTATTTTTCCAACAATTATTTATAAAGTAATGGAATGGCTGCTGGTTTTTTTGGGAAAAAAAAAAGTTTTCATTTTTGGGGTTTTCTTATGAACTCTTTTCTAAAAGAGTTAGAAAATCATCTGTTCAAGAGGAGGATTATACACAAGACCATGTTTTCAAAAGACAATTTATGTTAATATTGAAAAAAAGTTTTGTTTTGTGTTATTTTAGGGGCTCAAGATCCTTTTTTGCAGTTACCAATTCATCTCCCTTTTCACTTTGGGCCACTTTGTTTCTCTCTCTTTCTCTCTCTCCTCTGTTTTGTGGGTCAGGCTTAGATAAGGATCATAAAGAATGACAGATCTTCTTCACCTCCTCATTACAACAATGTTCTGAGATCAAACCAAAAAGTTGGGCTAAAAAGTCTCAATCTTTAACCTTTTTTTTTTCCTTTGCTTTTACAGGTTTTCTCATTCCAAAAGGATGGAGGATTTATGTGTACACCAGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACCTTCAACCCTTGGAGATGGCTGGTGGGTCTTTCTCTCTCTCTCTCTCTCTCTATCTCTCTCTACCCTTCTTTCTCTCTCTATTGGAAAATGACAATTTTGCCCTTGTGTGACATTTTCAGGACAAAAACTTGGAGTCCCATAATTACTTCTTCATCTTTGGAGGTGGAACCAGACATTGTCCTGGCAAAGAACTGGGAATTGCAGAGATTTCTACTTTTTTACATTACTTTGTAACTAAATACAGGTAGCTTTCTTTTTCTTATGCATTGTTTTGTTTGGATGCTAAGAAACAGCAAGAAAATGAAACAGAAGCTGAGATCAAGATTCAAACTTTTTTTTGTTTGTTTTGCAGATGGGAAGAAGTTGGAGGAGATAAGCTGTTGAAATTTCCAAGAGTGGAAGCTCCAAGTGGGTTCCACATTAGGGTTTCATCTTACTGACTGAATTAATTAATAATATTTATCCAAAAAAAAAAAAAAAAAAAAGAGTTAGTGTGAAGAATTGATGATGTACAGTAGGAGATGATGATATATAACCTTCTAACACCTTAGGGACAAAAGGAATGAAATTGAATTTTCAAGTGTTTACATCTCTCCTTTTTCTTTCCAATATTGCAACTTGGGCTTAGCTCAACAAAGGCCCAACCCACAATGATAATACCTTTGTTTTTTTTTTTTTTTGTGGGGCCTAACCC

mRNA sequence

ATTAACAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGAGCTTCTTGGGACCTTCAATGGCGCTGGCCTTAGCCATTCTTGCTCTGTTCTTGCTCCTCTGTTTTTGCTCTGCTTTACTGCGATGGAATGAAGTTCGATACAGTAAAAAGGGGCTGCCTCCTGGTACAATGGGCTGGCCTGTTTTTGGTGAAACCACTGAGTTTCTCAAACAAGGTCCTAACTTTATGAAATCCCAGAGAGCAAGGTATGGAAGCATTTTCAAGTCCCATATTTTGGGCTGTCCTACCATTGTTTCTATGGATCCTGAGGTTAATAGATATGTTTTAATGAATGAATCAAAAGGGCTTGTCCCTGGCTACCCTCAGTCCATGCTTGATATCCTTGGCAAATGCAATATCGCCGCCGTCCACGGCGGCGCTCACAAGCTGATGAGAGCTGCTCTGCTCGCCGTTGTCAGCCCCACCATGATTAAGGACCGTCTTCTTCCTAAAGTTGATGAATTTATGCGATCCCATTTGAGGAATTGGGATCAACAAATCATTGACATCCAGGACAAGACTAAAGAGATGGCTCTCCGGTCGTCTTTGAAGCAGATTGCCGGGATCGAGTCCGGTCCGTTATCGGAATCTTTCATCCCGGAGTTCTTTAAGCTTGTTCTCGGCACCCTCTCGCTCCCCATCGATCTCCCCGGCACCAACTACCGCCGAGGAATTCAGGCGAGGAAGAACATAATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAGAGACAAAGAAAGATGACATGCTTGGATATTTCATGAGTGAAGAGAACAAATACAAGCTAAACGATGAAGAGATTATCGATCAGATCATAACAGTTCTGTACTCTGGCTATGAAACTGTTTCGACAACTTCTATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAAATCAGAGAAGAGCATTTGGCAATCAGAGAAAAGAAAAATCCTGAGGACCCCATTGATTGGGATGATCTTAAAGCCATGAAGTTCACTCGTGCGGTGATATTTGAAACATCAAGGCTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACAAAGGACATGGAACTAAATGGTTTTCTCATTCCAAAAGGATGGAGGATTTATGTGTACACCAGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACCTTCAACCCTTGGAGATGGCTGGACAAAAACTTGGAGTCCCATAATTACTTCTTCATCTTTGGAGGTGGAACCAGACATTGTCCTGGCAAAGAACTGGGAATTGCAGAGATTTCTACTTTTTTACATTACTTTGTAACTAAATACAGATGGGAAGAAGTTGGAGGAGATAAGCTGTTGAAATTTCCAAGAGTGGAAGCTCCAAGTGGGTTCCACATTAGGGTTTCATCTTACTGACTGAATTAATTAATAATATTTATCCAAAAAAAAAAAAAAAAAAAAGAGTTAGTGTGAAGAATTGATGATGTACAGTAGGAGATGATGATATATAACCTTCTAACACCTTAGGGACAAAAGGAATGAAATTGAATTTTCAAGTGTTTACATCTCTCCTTTTTCTTTCCAATATTGCAACTTGGGCTTAGCTCAACAAAGGCCCAACCCACAATGATAATACCTTTGTTTTTTTTTTTTTTTGTGGGGCCTAACCC

Coding sequence (CDS)

ATGGCGCTGGCCTTAGCCATTCTTGCTCTGTTCTTGCTCCTCTGTTTTTGCTCTGCTTTACTGCGATGGAATGAAGTTCGATACAGTAAAAAGGGGCTGCCTCCTGGTACAATGGGCTGGCCTGTTTTTGGTGAAACCACTGAGTTTCTCAAACAAGGTCCTAACTTTATGAAATCCCAGAGAGCAAGGTATGGAAGCATTTTCAAGTCCCATATTTTGGGCTGTCCTACCATTGTTTCTATGGATCCTGAGGTTAATAGATATGTTTTAATGAATGAATCAAAAGGGCTTGTCCCTGGCTACCCTCAGTCCATGCTTGATATCCTTGGCAAATGCAATATCGCCGCCGTCCACGGCGGCGCTCACAAGCTGATGAGAGCTGCTCTGCTCGCCGTTGTCAGCCCCACCATGATTAAGGACCGTCTTCTTCCTAAAGTTGATGAATTTATGCGATCCCATTTGAGGAATTGGGATCAACAAATCATTGACATCCAGGACAAGACTAAAGAGATGGCTCTCCGGTCGTCTTTGAAGCAGATTGCCGGGATCGAGTCCGGTCCGTTATCGGAATCTTTCATCCCGGAGTTCTTTAAGCTTGTTCTCGGCACCCTCTCGCTCCCCATCGATCTCCCCGGCACCAACTACCGCCGAGGAATTCAGGCGAGGAAGAACATAATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAGAGACAAAGAAAGATGACATGCTTGGATATTTCATGAGTGAAGAGAACAAATACAAGCTAAACGATGAAGAGATTATCGATCAGATCATAACAGTTCTGTACTCTGGCTATGAAACTGTTTCGACAACTTCTATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAAATCAGAGAAGAGCATTTGGCAATCAGAGAAAAGAAAAATCCTGAGGACCCCATTGATTGGGATGATCTTAAAGCCATGAAGTTCACTCGTGCGGTGATATTTGAAACATCAAGGCTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACAAAGGACATGGAACTAAATGGTTTTCTCATTCCAAAAGGATGGAGGATTTATGTGTACACCAGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACCTTCAACCCTTGGAGATGGCTGGACAAAAACTTGGAGTCCCATAATTACTTCTTCATCTTTGGAGGTGGAACCAGACATTGTCCTGGCAAAGAACTGGGAATTGCAGAGATTTCTACTTTTTTACATTACTTTGTAACTAAATACAGATGGGAAGAAGTTGGAGGAGATAAGCTGTTGAAATTTCCAAGAGTGGAAGCTCCAAGTGGGTTCCACATTAGGGTTTCATCTTACTGA

Protein sequence

MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASETKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY

Homology

[Close]

BLAST of Lcy09g003190 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 750.7 bits (1937), Expect = 9.5e-216
Identity = 358/464 (77.16%), Postives = 416/464 (89.66%), Query Frame = 0


Query: 1   MALALAIL-ALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKS 60
           MAL +AIL  L LLLCFCSALL+WNEVR+ +KGLPPG MGWPVFGETTEFLKQGPNFMK+
Sbjct: 1   MALFMAILGVLVLLLCFCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPNFMKN 60

Query: 61  QRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           +RARYGS FKSHILGCPTIVSMDPE+NR++LMNE+KGLVPGYPQSMLDILG  NIAAVHG
Sbjct: 61  KRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILGTRNIAAVHG 120

Query: 121 GAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQ 180
             HK MR ALL+++SPT+I+D+LLPK+DEFMR+HL +WD ++I+IQ+KTKEMA  SSLKQ
Sbjct: 121 STHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKVINIQEKTKEMAFLSSLKQ 180

Query: 181 IAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRAS 240
           IAG+ES  +++ F+ EFFKLVLGTLSLPI+LP TNYR G+QARK+II +L QL+ ER+AS
Sbjct: 181 IAGMESSSIAQPFMTEFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQLLEERKAS 240

Query: 241 ETKKDDMLGYFM-SEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +    DMLG  M  +EN+YKL+DEEIID +IT++YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 QDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 360
           +IR+EH AIRE+K PEDPID +DLK+M+FTRAVIFETSRLATIVNGVLRKTT DMELNG+
Sbjct: 301 EIRKEHFAIRERKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTHDMELNGY 360

Query: 361 LIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELG 420
           LIPKGWRIYVYTREINYDPFLY +P TFNPWRWL  +LES ++F IFGGGTR CPGKELG
Sbjct: 361 LIPKGWRIYVYTREINYDPFLYHDPLTFNPWRWLGNSLESQSHFLIFGGGTRQCPGKELG 420

Query: 421 IAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           IAEISTFLHYFVT+YRWEEVGGDKL+KFPRV AP+G HIRVSS+
Sbjct: 421 IAEISTFLHYFVTRYRWEEVGGDKLMKFPRVVAPNGLHIRVSSF 464

[Close]

BLAST of Lcy09g003190 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 1.6e-210
Identity = 345/464 (74.35%), Postives = 412/464 (88.79%), Query Frame = 0


Query: 1   MALALAILALFLLLC-FCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKS 60
           MA  L  L+ F  LC FC+ALLRWN+V+Y++K LPPGTMGWP+FGETTEFLK GP+FMK+
Sbjct: 1   MAFFLIFLSSFFGLCIFCTALLRWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPSFMKN 60

Query: 61  QRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QRARYGS FKSHILGCPTIVSMD E+NRY+L+NE+KGLVPGYPQSM+DILGKCNIAAV+G
Sbjct: 61  QRARYGSFFKSHILGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMIDILGKCNIAAVNG 120

Query: 121 GAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQ 180
            AHK MR ALL+++SPTMI+D+LLPK+DEFMRSHL NWD ++IDIQ+KT +MA  SSLKQ
Sbjct: 121 SAHKYMRGALLSLISPTMIRDQLLPKIDEFMRSHLTNWDNKVIDIQEKTNKMAFLSSLKQ 180

Query: 181 IAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRAS 240
           IAGIES  L++ F+ EFF LVLGTLSLPI+LP TNY RG QARK I+ LL+ LI ERRAS
Sbjct: 181 IAGIESTSLAQEFMSEFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRAS 240

Query: 241 ETKKDDMLGYFMSEE-NKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +  + DMLGY M+EE  ++KL D+E+ID IIT+LYSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 KEIQHDMLGYLMNEEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 360
           ++R+EH+AIREKK PEDPID++D ++M+FTRAVI ETSRLATIVNGVLRKTT+DME+NG+
Sbjct: 301 ELRKEHMAIREKKKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGY 360

Query: 361 LIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELG 420
           +IPKGWRIYVYTRE+NYDP LY +P++FNPWRW+DK+LE  N F +FGGGTR CPGKELG
Sbjct: 361 IIPKGWRIYVYTRELNYDPRLYPDPYSFNPWRWMDKSLEHQNSFLVFGGGTRQCPGKELG 420

Query: 421 IAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           +AEISTFLHYFVTKYRWEE+GGDKL+KFPRVEAP+G  IRVS++
Sbjct: 421 VAEISTFLHYFVTKYRWEEIGGDKLMKFPRVEAPNGLRIRVSAH 464

[Close]

BLAST of Lcy09g003190 vs. ExPASy Swiss-Prot
Match: Q940V4 (Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 9.9e-197
Identity = 324/465 (69.68%), Postives = 392/465 (84.30%), Query Frame = 0


Query: 1   MALALAILALF-LLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKS 60
           M + + IL L  +++C C+ALLRWN++RYSKKGLPPGTMGWP+FGETTEFLKQGP+FMK+
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  QRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSHILGCPTIVSMD E+NRY+LMNESKGLV GYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 GAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++SPTM+KD LLPK+D+FMR++L  WD  + +DIQ+KTK MA  SSL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIA     P  E +  EFFKLV+GTLS+PID+PGTNYR G+QAR NI +LL +L+ ER+ 
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SETKKDDMLGYFM-SEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGY M  E+N+Y L D+EI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           +++R EHLAIRE+K P++P+  DD+K+MKFTRAVIFETSRLATIVNGVLRKTT D+ELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKEL 420
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW++K+LES +YF +FGGG R CPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           GI+E+S+FLHYFVTKYRWEE G DKL+ FPRV AP G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

[Close]

BLAST of Lcy09g003190 vs. ExPASy Swiss-Prot
Match: Q9FMA5 (Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.4e-195
Identity = 321/463 (69.33%), Postives = 390/463 (84.23%), Query Frame = 0


Query: 2   ALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQR 61
           A+ + +  L +++  CSALLRWN++RY+K GLPPGTMGWP+FGETTEFLKQGPNFM++QR
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGA 121
            RYGS FKSH+LGCPT++SMD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLKQI 181
           H+LMR +LL+++S TM++D +LPKVD FMRS+L  W++ ++IDIQDKTK MA  SSL QI
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 241
           AG    P  E F   FFKLV+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 TKKDDMLGYFMSEE-NKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLGY M +E N+Y L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 IREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 361
           +R EHLA RE+K  ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+L
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

Query: 362 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGI 421
           IPKGWRIYVYTREINYD  LY +P  FNPWRW+ K+LES N  F+FGGGTR CPGKELGI
Sbjct: 363 IPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGI 422

Query: 422 AEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
            EIS+FLHYFVT+YRWEE+GGD+L+ FPRV AP GFH+R+S Y
Sbjct: 423 VEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

[Close]

BLAST of Lcy09g003190 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 3.1e-190
Identity = 316/467 (67.67%), Postives = 386/467 (82.66%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSA-LLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKS 60
           MA+ L I  +F   C  S  L RW ++ Y+KK LPPGTMGWP+FGET EFL QGPNFMK+
Sbjct: 1   MAIFLIIFVVFFGFCILSTPLFRWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPNFMKN 60

Query: 61  QRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QRARYG+ FKSHILGCPT+VSMD  +N Y+L NE+KGL+PGYPQSMLDILGKCNIAAVHG
Sbjct: 61  QRARYGNFFKSHILGCPTVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILGKCNIAAVHG 120

Query: 121 GAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLK 180
             HK +R ALL++++PTMIKD +LPK+D+FMRSHL  WD   +IDIQ  TKEMA  SSL 
Sbjct: 121 ATHKYIRGALLSLINPTMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLD 180

Query: 181 QIAGI-ESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERR 240
           QI G   S  +++ F   F  + LGT+SLPI+ P TNY RG+Q RK I+KLL+++I +RR
Sbjct: 181 QIGGFATSSSIAQEFRAGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRR 240

Query: 241 ASETKKDDMLGYFMSEE--NKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPK 300
            S+  + DMLG  M+EE  N+Y L+DEE+IDQIIT++YSG+ETVSTTSMMAVK+LHDHPK
Sbjct: 241 GSKKIQQDMLGLMMNEEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPK 300

Query: 301 VLQQIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMEL 360
            L++IR+EH AIREKK+ EDPID++D KAM+FTRAVI+ET RLATIVNGVLRKTT+DMEL
Sbjct: 301 ALEEIRKEHFAIREKKSLEDPIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMEL 360

Query: 361 NGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGK 420
           NG++IPKGWRIYVYTRE+NYDP +Y +P+TFNPWRWL+ NL+  + F +FGGGTR CPGK
Sbjct: 361 NGYMIPKGWRIYVYTRELNYDPLIYPDPYTFNPWRWLENNLDHQSSFLMFGGGTRLCPGK 420

Query: 421 ELGIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           ELG+AEISTFLHYFVT+YRWEEVGG+KL+KFPRVEA +G  I+VS+Y
Sbjct: 421 ELGVAEISTFLHYFVTRYRWEEVGGNKLMKFPRVEALNGLWIKVSAY 467

[Close]

BLAST of Lcy09g003190 vs. ExPASy TrEMBL
Match: A0A6J1C1V2 (cytochrome P450 85A-like OS=Momordica charantia OX=3673 GN=LOC111006661 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.7e-261
Identity = 441/462 (95.45%), Postives = 458/462 (99.13%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MA+A+AILAL LLLCFCSALLRWNEVR+SKKGLPPGTMGWPVFGETTEFLKQGPNFMK+Q
Sbjct: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+VSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALLA+VSP MIKDRLLPKVD+FMRSHLRNWD+QIIDIQDKTKEMALRSSLKQI
Sbjct: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKL DEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+KNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. ExPASy TrEMBL
Match: A0A6J1JRL1 (cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111487713 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 7.7e-261
Identity = 442/462 (95.67%), Postives = 456/462 (98.70%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+AILALFLLLCFCSALLRWNEVRY +KGLP GTMGWP FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAIAILALFLLLCFCSALLRWNEVRYRRKGLPHGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL+VVSPTMI+DRLLPKVD+FMRSHLRNWDQQIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARK+IIKLLQQLINERRA+E
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKSIIKLLQQLINERRATE 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKLNDEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHLAIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. ExPASy TrEMBL
Match: A0A6J1GNX1 (cytochrome P450 85A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455792 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.5e-259
Identity = 439/462 (95.02%), Postives = 453/462 (98.05%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+AILAL LLLCFCSALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARY  +FKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYPFLFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL+VVSPTMI+DRLLPKVD+FMRSHLRNWDQQIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNI+KLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKLNDEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH AIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. ExPASy TrEMBL
Match: A0A6J1FN01 (cytochrome P450 85A-like OS=Cucurbita moschata OX=3662 GN=LOC111445417 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.2e-258
Identity = 440/462 (95.24%), Postives = 453/462 (98.05%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+ ILALFLLLCFC+ALLRWNEVRYSKKGLPPGTMGWP+FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIV MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALL+VVSPTMIKDRLLPKVDEFMRSHLRNWD+QIIDIQDKTKEMAL SSLKQI
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPID PGTNYR+GIQARK+IIKLLQ+LI+ERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKSIIKLLQKLIDERRASK 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKL DEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYD FLYSEP TFNPWRWLDKNLESHNYFFIFGGGTR CPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDSFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRQCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. ExPASy TrEMBL
Match: A0A6J1JUV6 (cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111489110 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.6e-258
Identity = 440/462 (95.24%), Postives = 452/462 (97.84%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+ ILALFLLLCFC+ALLRWNEVRYSKKGLPPGTMGWP+FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIV MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALL+VVSPTMIKDRLLPKVDEFMRSHLRNWD+QIIDIQDKTKEMAL SSLKQI
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPID PGTNYR+GIQARK IIKLLQ+LI+ERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKIIIKLLQKLIDERRASK 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKL DEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYD FLYSEP TFNPWRWLDKNLESHNYFFIFGGGTR CPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDSFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRQCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. NCBI nr
Match: XP_038890036.1 (cytochrome P450 85A-like [Benincasa hispida])

HSP 1 Score: 919.1 bits (2374), Expect = 1.5e-263
Identity = 448/462 (96.97%), Postives = 456/462 (98.70%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+ ILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAMVILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHKLMR ALLA+VSPTMIKDRLLPKVD+FMRSHLRNWD QIIDIQ+KTKEMALRSSLKQI
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKVDDFMRSHLRNWDSQIIDIQEKTKEMALRSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERR S+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRDSK 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
            +KDDMLG+ MSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 IQKDDMLGFLMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. NCBI nr
Match: XP_023524460.1 (cytochrome P450 85A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911.8 bits (2355), Expect = 2.5e-261
Identity = 444/462 (96.10%), Postives = 455/462 (98.48%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALALAILALFLLLCFCSALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL+VVSPTMI+DRLLPKVD+FMRSHLRNWDQQIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKLNDEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH AIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYD FLYSEP TFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDAFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. NCBI nr
Match: KAG7033215.1 (Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 911.4 bits (2354), Expect = 3.2e-261
Identity = 442/462 (95.67%), Postives = 456/462 (98.70%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+AILAL LLLCFCSALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIVSMDPEVNRY+LMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL+VVSPTMI+DRLLPKVD+FMRSHLRNWDQQIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLL+QLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLINERRASE 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKLNDEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHLAIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. NCBI nr
Match: KAG6602538.1 (Cytochrome P450 85A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910.6 bits (2352), Expect = 5.5e-261
Identity = 441/462 (95.45%), Postives = 455/462 (98.48%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MALA+AILAL LLLCFCSALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQGPNFMKSQ
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTIVSMDPEVNRY+LMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL+VVSPTMI+DRLLPKVD+FMRSHLRNWDQQIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNI+KLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKLNDEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH AIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. NCBI nr
Match: XP_022134388.1 (cytochrome P450 85A-like [Momordica charantia])

HSP 1 Score: 910.6 bits (2352), Expect = 5.5e-261
Identity = 441/462 (95.45%), Postives = 458/462 (99.13%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60
           MA+A+AILAL LLLCFCSALLRWNEVR+SKKGLPPGTMGWPVFGETTEFLKQGPNFMK+Q
Sbjct: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60

Query: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+VSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALLA+VSP MIKDRLLPKVD+FMRSHLRNWD+QIIDIQDKTKEMALRSSLKQI
Sbjct: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240

Query: 241 TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQI 300
           T+KDDMLG+FMSEENKYKL DEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ+
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+KNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420
           PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Lcy09g003190 vs. TAIR 10
Match: AT3G30180.1 (brassinosteroid-6-oxidase 2 )

HSP 1 Score: 687.6 bits (1773), Expect = 7.0e-198
Identity = 324/465 (69.68%), Postives = 392/465 (84.30%), Query Frame = 0


Query: 1   MALALAILALF-LLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKS 60
           M + + IL L  +++C C+ALLRWN++RYSKKGLPPGTMGWP+FGETTEFLKQGP+FMK+
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  QRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSHILGCPTIVSMD E+NRY+LMNESKGLV GYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 GAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++SPTM+KD LLPK+D+FMR++L  WD  + +DIQ+KTK MA  SSL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIA     P  E +  EFFKLV+GTLS+PID+PGTNYR G+QAR NI +LL +L+ ER+ 
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SETKKDDMLGYFM-SEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGY M  E+N+Y L D+EI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           +++R EHLAIRE+K P++P+  DD+K+MKFTRAVIFETSRLATIVNGVLRKTT D+ELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKEL 420
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW++K+LES +YF +FGGG R CPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
           GI+E+S+FLHYFVTKYRWEE G DKL+ FPRV AP G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

[Close]

BLAST of Lcy09g003190 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 683.7 bits (1763), Expect = 1.0e-196
Identity = 321/463 (69.33%), Postives = 390/463 (84.23%), Query Frame = 0


Query: 2   ALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQR 61
           A+ + +  L +++  CSALLRWN++RY+K GLPPGTMGWP+FGETTEFLKQGPNFM++QR
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGA 121
            RYGS FKSH+LGCPT++SMD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLKQI 181
           H+LMR +LL+++S TM++D +LPKVD FMRS+L  W++ ++IDIQDKTK MA  SSL QI
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 241
           AG    P  E F   FFKLV+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 TKKDDMLGYFMSEE-NKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLGY M +E N+Y L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 IREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 361
           +R EHLA RE+K  ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+L
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

Query: 362 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGI 421
           IPKGWRIYVYTREINYD  LY +P  FNPWRW+ K+LES N  F+FGGGTR CPGKELGI
Sbjct: 363 IPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGI 422

Query: 422 AEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463
            EIS+FLHYFVT+YRWEE+GGD+L+ FPRV AP GFH+R+S Y
Sbjct: 423 VEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

[Close]

BLAST of Lcy09g003190 vs. TAIR 10
Match: AT5G38970.3 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 566.6 bits (1459), Expect = 1.8e-161
Identity = 270/393 (68.70%), Postives = 330/393 (83.97%), Query Frame = 0


Query: 2   ALALAILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQR 61
           A+ + +  L +++  CSALLRWN++RY+K GLPPGTMGWP+FGETTEFLKQGPNFM++QR
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGA 121
            RYGS FKSH+LGCPT++SMD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLKQI 181
           H+LMR +LL+++S TM++D +LPKVD FMRS+L  W++ ++IDIQDKTK MA  SSL QI
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 241
           AG    P  E F   FFKLV+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 TKKDDMLGYFMSEE-NKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLGY M +E N+Y L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 IREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 361
           +R EHLA RE+K  ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+L
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

Query: 362 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWL 393
           IPKGWRIYVYTREINYD  LY +P  FNPWRW+
Sbjct: 363 IPKGWRIYVYTREINYDANLYEDPLIFNPWRWM 395

[Close]

BLAST of Lcy09g003190 vs. TAIR 10
Match: AT5G38970.2 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 560.5 bits (1443), Expect = 1.3e-159
Identity = 267/384 (69.53%), Postives = 321/384 (83.59%), Query Frame = 0


Query: 81  MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGAHKLMRAALLAVVSPTMIKD 140
           MD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG +H+LMR +LL+++S TM++D
Sbjct: 1   MDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRD 60

Query: 141 RLLPKVDEFMRSHLRNWDQ-QIIDIQDKTKEMALRSSLKQIAGIESGPLSESFIPEFFKL 200
            +LPKVD FMRS+L  W++ ++IDIQDKTK MA  SSL QIAG    P  E F   FFKL
Sbjct: 61  HILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKL 120

Query: 201 VLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASETKKDDMLGYFMSEE-NKYK 260
           V+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR S     DMLGY M +E N+Y 
Sbjct: 121 VVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSGETFTDMLGYLMKKEGNRYP 180

Query: 261 LNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQIREEHLAIREKKNPEDPID 320
           L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ++R EHLA RE+K  ++P+ 
Sbjct: 181 LTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLG 240

Query: 321 WDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLIPKGWRIYVYTREINYDPF 380
            +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+LIPKGWRIYVYTREINYD  
Sbjct: 241 LEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDAN 300

Query: 381 LYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIAEISTFLHYFVTKYRWEEV 440
           LY +P  FNPWRW+ K+LES N  F+FGGGTR CPGKELGI EIS+FLHYFVT+YRWEE+
Sbjct: 301 LYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRWEEI 360

Query: 441 GGDKLLKFPRVEAPSGFHIRVSSY 463
           GGD+L+ FPRV AP GFH+R+S Y
Sbjct: 361 GGDELMVFPRVFAPKGFHLRISPY 384

[Close]

BLAST of Lcy09g003190 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 3.7e-90
Identity = 175/468 (37.39%), Postives = 272/468 (58.12%), Query Frame = 0


Query: 5   LAILALFLLLCFCSA--LLRWNEVRYSKKGLPPGTMGWPVFGETTEFL----KQGPN-FM 64
           +A  A  LLL   +A  LL     RY + GLPPG++G P+ GET + +     + P  F+
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFI 60

Query: 65  KSQRARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAV 124
             + ARYGS+F +H+ G PTI S DPE NR+VL NE K     YP S+ ++LGK ++  +
Sbjct: 61  DERVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLM 120

Query: 125 HGGAHKLMRAALLAVVSPTMIKDRLLPKVDEFMRSHLRNWDQQIIDIQDKTKEMALRSSL 184
            G  HK M +  ++  + ++IKD L+  +D  +R +L +W  +++ + ++ K++    ++
Sbjct: 121 KGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVL-LMEEAKKITFELTV 180

Query: 185 KQIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERR 244
           KQ+   + G  SES   E+  ++ G  SLP+ L  T YR+ IQAR+ + + L  ++ +RR
Sbjct: 181 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRR 240

Query: 245 ASE----TKKDDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDH 304
             E     +K DML   ++ ++ +  +DEEI+D ++ +L +GYET ST   +AVKFL + 
Sbjct: 241 EEEEEGAERKKDMLAALLAADDGF--SDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET 300

Query: 305 PKVLQQIREEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDM 364
           P  L Q++EEH  IR  K+    ++W D K+M FT+ V+ ET R+A I+ GV R+   D+
Sbjct: 301 PLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDV 360

Query: 365 ELNGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLES--HNYFFIFGGGTRH 424
           E+ G+ IPKGW+++   R ++ DP  + +  TFNPWRW   ++ +   N F  FGGG R 
Sbjct: 361 EIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRL 420

Query: 425 CPGKELGIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRV 460
           CPG EL    +S FLH  VT + W     DKL+ FP       + I V
Sbjct: 421 CPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFV 465

The following BLAST results are available for this feature:

BLAST of Lcy09g003190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69F95	9.5e-216	77.16	Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2	[more]
Q43147	1.6e-210	74.35	Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1	[more]
Q940V4	9.9e-197	69.68	Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1	[more]
Q9FMA5	1.4e-195	69.33	Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1	[more]
Q50LE0	3.1e-190	67.67	Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1	[more]

BLAST of Lcy09g003190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1V2	2.7e-261	95.45	cytochrome P450 85A-like OS=Momordica charantia OX=3673 GN=LOC111006661 PE=3 SV=...	[more]
A0A6J1JRL1	7.7e-261	95.67	cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111487713 PE=3 SV=1	[more]
A0A6J1GNX1	1.5e-259	95.02	cytochrome P450 85A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11145579...	[more]
A0A6J1FN01	1.2e-258	95.24	cytochrome P450 85A-like OS=Cucurbita moschata OX=3662 GN=LOC111445417 PE=3 SV=1	[more]
A0A6J1JUV6	3.6e-258	95.24	cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111489110 PE=3 SV=1	[more]

BLAST of Lcy09g003190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890036.1	1.5e-263	96.97	cytochrome P450 85A-like [Benincasa hispida]	[more]
XP_023524460.1	2.5e-261	96.10	cytochrome P450 85A-like [Cucurbita pepo subsp. pepo]	[more]
KAG7033215.1	3.2e-261	95.67	Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6602538.1	5.5e-261	95.45	Cytochrome P450 85A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022134388.1	5.5e-261	95.45	cytochrome P450 85A-like [Momordica charantia]	[more]

BLAST of Lcy09g003190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G30180.1	7.0e-198	69.68	brassinosteroid-6-oxidase 2	[more]
AT5G38970.1	1.0e-196	69.33	brassinosteroid-6-oxidase 1	[more]
AT5G38970.3	1.8e-161	68.70	brassinosteroid-6-oxidase 1	[more]
AT5G38970.2	1.3e-159	69.53	brassinosteroid-6-oxidase 1	[more]
AT5G05690.1	3.7e-90	37.39	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 369..393 score: 29.21 coord: 329..347 score: 30.9 coord: 402..412 score: 45.3 coord: 61..80 score: 25.25 coord: 412..435 score: 23.57
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 274..291 score: 29.56 coord: 412..423 score: 31.72 coord: 330..341 score: 41.33 coord: 403..412 score: 46.66
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 34..442 e-value: 2.4E-61 score: 207.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..459 e-value: 3.2E-104 score: 351.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 27..442
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 5..441 e-value: 6.6E-21 score: 72.6
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 7..460
None	No IPR available	PANTHER	PTHR24286:SF273	CYTOCHROME P450 FAMILY PROTEIN	coord: 7..460
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 405..414

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g003190.1	Lcy09g003190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen