Lcy09g003180 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g003180
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
LocationChr09: 2707986 .. 2717647 (+)
RNA-Seq ExpressionLcy09g003180
SyntenyLcy09g003180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCAGAAACAGAAATCATGGTTCAGCCCCTAATCCATTCTTGTTGGTGTCTCACCACTCATTTTTCAGCCCCTAACTCGTGCTCCCAGGTTTTGATTTTTTCGTTTTATCTTGTTTAGTTGATTTGTTCTGTTCAAAACCAAAGAAGAAATTCAAAAGAAGCAACTTTTTTAGGTAAATAAAACAAAAAAGGAATCAATATATAAAGAGGAACAAAAAAAAAAAGAACAAACTATTCTTGTGGTAGGGAGGCGTTTGCTTGAATATAGAAAAAAATACTATACTAGGGAGTCGTAAATTAAAAAAAAAAAAAAGAAAATCTCTATAGGATAAATTAAAATAAAAACTATCGTCGGGTAAACCTTCTGTCATTCTATTTTTTCTAGCTATCTATTTTATTTTACCATTTATGTTTCTTTATCGTTTATTTGTCACGTTCAAATTAACTAATCATCATTTAAGAGACATGCCATAATTATATAATCATCAATGTAGTTAAGCATGCAATATCACGATTAACTGTTCAAACATGTATTGGTTGGTTACTGCTTAGTGGTTGATTTTGTGTATGTAACTGCTGCCCTGCTTGTTTCAAGCTCTGAAACTCTTCCACGAAGGTCTTAGGAGCAAAAGAAGCGGAGACATTGGAGCTTTTTCAGGGTTGTGGATTTTTCTTTGACCCTTGAAACAAGCCAAGGAAAGGAAGAAGAAAGAATCCCAGTATCTGTTTTTTTCATGTGAGACTGAGAGCCGCAATAATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTTTTTTTCAATAAGACCCAGGTGCCTTGTTTATTTAATAATGGTTTTTTGGTGTTTTGGAGTTTAGGCATTGTTCTTAGTTAAAATTTATCGGTTAGTCAAAGTTTCCAATCCATGGATTCTAGGGGTGGAGAAAGAATTTACATACTATTGTTTATAGCATTTGAAGTTTGGCGTGGTTATTGATTGGGGAAATATTTAAGATTTGACTTCTATTCTTATTTAAGTGGGAAAATTTCATGAGGGAGAAGGGGTAGCTTAGGGAAAACTTCTATTTTGGTGAAGTGAGGAGGCCTTGTAATTTTTGCTAGTATCTTGAAACTTCAGCATGTTAGCATATATAAGTGATTGATATTTGATGGAGAAACTAAGATATACAATTTAATCAAGTCAGCGATTATAATGTAGTATGGTAAGTTTAAAGATATTGTCCCGTGTAGTATGCACAACATGACAGCAATCTCCTTAATCTTATGAATAACTTATCGTTCTTTTTAAGATTCTTAGCGACAATATTAGCTAAATTATTTTCCTCAAGCTGCACTACTTTTACTACAAAATTTGTTAGTCCCAGCAGAAATCTTCCCCTCAGTAATTAAGAGTATCTGGAAATTTTTCTCCCTTTATGAAAACTATTACCTACTTTTTTCTATCTGTAATATCATCATAGAATTTAGTGGTTGTTGGATTTTGATTTCCCTTTTTTTCTCTATACTGCCCACATATACCTAGCTCAAGCTTTCTCTCATTGTCAAATCTTGACTGTTCACCTCTGTAATACTCAGGTTAACAACAGAGACATATCTATTGCTGTTCTTAGGACATTTATATCAAAACGCAGGCAGGAGCATGAAGCAAAACTATCAAGAAGAGCCAAATCAGGGCAAAAGATTTCTGAAAATAATGGTTCTGAAACTGTTATGGAACAATCATCTAATGAATCTGCAACAAACAATGGAAAGTGCAATACAGATTGTGACGCTCATGAAAAAACATCACGTGAATGTGGAAGTCCGATGTTGTCAGAAGAACAGAAAGAGATCACAGAGGGAAAAGAACAAAGAGATCCCAAGCCTCTTAGAGTTCTCGAGGTTCTTTTTAATTTATCATTTTAATATGTACATGTGCATAATTTAATTGAAGTTTATTGTCCAATGAAGTGGTAGACATTTCAATTTAGGATTATGTTCGTGCCTGGATTATTAGCTCCCTGTTTGAGTAATATAGAGTTTATAGATCCCTTTCCCCCCTACAAAGGCAACCAAAATTCTGAAATGATAAGTACCTGCTAGAGATGCTCAACAAGAAGTTTGGCGACAATTTATTAATTAACAGTGGATAAACTAAGAGGCCAGAAGCTTTCACTTCTCTTACAGTGGAACTTTTATGAACAAGATGACTGAGAGTTTCGTGAGCACTAAAATTGAAAATTCCTTTATGAAAAATTTTTAGAACAATGCAATGTAGCTGGGGGTTTTTCCAACTCTAAGTTCTTAAATGGTTGCAATATTTATTCTCCATCAAACGCCTCCTCCAATTGTTAGCTACCTTGAATTGAATTGGCCACTAGGACCTAATTTTTTTTTTTTGAACAAATGGAAGACCAAGGGAAAGGTTAAGGGCATAACAGAAAAAAATATAAAATGATGAATCATGAATGAAGATAGCACTATGTATCTGATATCTAAAATTTTGCTTCTTATATGATAGTTGGGTCATTAGTTTAGAGTTTTTCTTATTTTATTATAAATCTGTTGTTTTTCTATAAGAAACAGAGAAATGTTTTTTTTTGGGGATAAAAGTATCATATACTTTTATTTTTATTTTAAATCTGATCTTGGTTTTCCCATGTGTACTTTTGCTATAGATCACTGCATTGTTAAACATTATGTAATTGGCTTCTCAAATTCTTACAGATGCTGTATAGCTTATCATCTCATTCACATAGCAGTTGAAATTTCAAGTATCACAATATAAATTGTATCTTTTATGTGAAAATTATGTAGATGCATCAAAAGATACAAATATATTTATAAAAGTTGTTCTAAATAGTGTATAGATGCATATTCATGAATATGAATGCGTCCCTGTTCTAACATATTAGAATTTACTGTTCAACATGTTGTCATTGTGTTTCTTGATTCCAACACCGGCATTATATTTGTGCTTCTAAACTTTTGTTTGTGTGTTAGCTTATCCTTTTGGGATGCCTGGTATGAGTTGCTTATCTTTTGGGATGTCTGGTATGGGTTTTGGTGATACGTTGTCCATTCACTACTTTCTGAGTAATTCCTGTTTGAATTTGAAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGGATTGATCACGTTGTGGCGCTAGACAACGACAAAGGTAATTTTTAAAATTATTTGTAATATCAAAAATTTCTTTTTAGCAAATATGTTTACTTGGCCATTCTTCAGGAGGGGTGGGGTATGTAATAAGTGGAATCAGAAATGTCACCTTTGGTTGACACTACCCCTAATATCTAAGGCAAGTTTACCTTGTACAAGCAGAGGAAGTTATTGTTGATAATTCTTTCCCCTTCTTATCTCTGTTGACTTATGTATTCATCTTCTGGAGAGCATGTATTAATGTCTCAGATTGCCAGTGGAACCTTAGAAATGGAAGTTTTCCATTTTACTAATCTTACAATATGCTCTTAAGTCTTATCTATATTGATATGTTGCTCTGGGTGTTTCTTTTAGTTATCAGTTACTAACCTGTTAACGATAAGAATTGTTTTTCAACAGCATCGGTTGAAGCTTGTCAGAGAAACATCAAGTTTAATGGTTCAGTAGCGTGTGCAAAAGTGGAATCACATCTTACTGATGCTCGTGTATATATGCTCACCCACCCAAAAGAATTTGATATGGTATGCACACACTATTTCAGATATGTTGGTTAGCCCTTTAGACATTACATATAGGGAAGTAACTACTGAAATGGAATTTTTTTTCTTCCTTATTATAGTTCAGACTTTGGAATAGTAACCATTTGAATAATTATATTATATATGTAATGATATGAGCACCAACGTGAATATAAGATTGATGCTTTTATGCAACTTATTTGGACTGTTTCAGGTTGATCTGGATCCTTATGGTTCACCTTCTATTTTCTTGGACTCTGCCGTTCAATCAGTTGTCGATGGTGGCATGCTGATGTGCACAGCAACTGATATGGCAGTGCTCTGTGGGGGAAATGGAGAAGTTTGCTATTCGAAGTAAGGTTTTTAACAGTCGTCAAATATCCTTCTTTTTTTGAAGTTCTATTGTAATAGATTCTGTGTCCTAAAAATTGCTGATTGATTCAGATATGGTTCCTATCCTTTGAGAGGAAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGGTATACCTATCTCCTGTGTATTTCAAAAGTTTTCTTGATGAATTATTTTACCACCTTTTATTTTTTTCTTCTTTGGCTTAACATGTTGTACAGTGTACAAGATCATTCTTATCTTAGTAAAAACATGTAGTATAGGATCATAAATGATGCACATACACACACTATAGGAAACTGAAAATCTATTAAAAACGAGAGGATTACTAAAGGAGGGGAGGAGAAATCCCTCAAGGCAAAGGGAATTATGAACAAGGATCCCAATTTTGACAAATAAGCAAAATATTGTAATCACAAAAAGAATATAGGACGAGGTACACACCAAGAAGCATAGAAGTAGTAGGTCCAACATTCTTCCAAAGGAGTAAAAGAGTCTTTGAAAATCTTTTGAAGAGACAATTGACCACATTTTTCTACACTGCCCCTTTGCTGCAAATGGCTGGTTTTTTCTTTTGGATGCTTTTGGGTTGTCTTGCTGTTTTCCAAGCAAATTGATGGCTGGTTGGTGGAAGCCTAGCTAGTTGGAGGTTGACTAAACAAGCAATTATCCTTTGGAATTGTCTGGCTAGAGTGCTCCTTTGACATATTTGGTTGGAGTGGAATCTTCTTATTTTTAAAGATAAGGCTTCCTCCTTTGTTATTTTTTGTAGTAATGTACAACATACCTCTTCTTGGTGGTGTGTGGTGTTTTAATTGGAGGAAATTCCTTTTGTAATTATAGCCTTCTATGGTCATGAACAACTGGAAAGCTCTTCTTTAGTTCTTTTGTGGGTGTGGGTTCCCTTTCTTCTTTAGTTCTTTTGTGGGTGTAGGTCCTCTTTCTTCTTTAGTTCTTTTGTGGGTGTAGGTCCTCTTTCTTCTTTAGTTCTTTTGTGGGTGTAGGTCCTCTTTCTTCTTTAGTTCTTTTGTGGGTGTAGGTCCTCTCTTTCCCTAGCCCTTAGGCTGTTCTTCTTTAGTTCTTTTGTGGGTGTAGGTCCTCTCTTTCCCTAGCCCTTAGGCTGTTTTCTCTATTTTTTCAGTTAATATACTCAAGTTTCTTATAAAAAAAGAGAAGATCCTTCGATTCCTCTCCTCTCCTATCTAAGCCTCCATAAGTGAGGGTATTAGTCCGAAGGATTGTGGCCTTTCTGGTGAAAGAGTGTCAATGCAGAATCCGAGCTATCATCGTTCTGCAACATGAAGAGAAACACCAAGCAATTCCTGCTTATGAGCTTTTATCACTCAGAGAAACATAAGATGGTGATATCCAAGTTGATACCCACAGTATTATCAATAAAGTATTTAGCTCTGCCTGTTTATGAGCATTTTGCACTCAAGAAATAATTTACTTTCTTTATTTTTTAGTTAATTTGAGATCAGTCATATAGTGCTTCACCTGCCTCCTCATTCTAATCTCAGAACATGCAAGCCTCTTTAACTAAATACAACATTCTTCAAATTTTCCATCTCACTAACAAGCAGAAGTGGTCTCTTATGCATGATTATCATTGTTCTAAATTCTTTGTAGAGCCATGCGAATAGATACAAGCGGTATATTGTTCCGGTGCTATCTGTGCAGATGGACTTCTATGTTCGGGTTTTTGTTCGTATTTATACGTAAGTTGCCTACGCCTTTGAATTTACTGGGTTAGCTATAATGCACCCTTTTTTTTTTCCTGTGTGTGTGTGTGTTATAAGTATTTATATAAAGATGGTGTAGTTTCATTTGATTGTTTACACTTGGTGAAATAATTGGCTGCAGTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGGAGAAGCGTCTCCAAGGTATATTTCTCTACGACCTGGTATTTATGCTGGTTAATCAGATTATTGAGTTTATTTTTATTATCCCGTGTAATTTCTCAAACTGGTGCAAGTTTCTCCTGCTTATTTAAATATTTATACTTATACACTTACCCCCTGCCCCCTTTATTCTTTTGAACTACTAGTTTTCATTGAGTCAAATGAAAGTAAAACAAACAAGCGCACGAGAATAAAACCGCCGGCCAAAAGTGAACACATATAGCAAATTATGATAATGGGCATTAACTTTTGGTGATAAAAATTTATTCCAAAAATTGCTTTGAACTATGAGCGCAAAGGGAGCCATTAAAATGCACCAACCCTCACACATCTTCCCACTACCTGTCTAATTCCACAAACAGTCTCCCATTTCTAAAGCTAGTTTCCTGAAGTAAAATGATGTCCTCCATCATCCCTCAAATGCTTGGACTTCTAAACCAGATAAATATCAAAAACATGTCACTAGCAACTCAAGCAAGGGGGTATTACCAAAGGAGGTGATTAAGATCCTCTGCATCAAACTCGCATCAAGCACACCAATGGTTCTTGGCTGGAAGTCAACATTTACAAGCTAAGAAAATAACTGAATTTTCATGAAAATCTATCCTAATCTTTTGAATATGCTGCTCGGATTTACTTCTTTCCAAGCTTTGGACTAAGGGACCCAATTCTCAGGATTGGCTTACTTGTTTGTTTTGTTGTAACCTCATACTTGATTATGATTTATGAGAGGTTGGAAAGAGAGAAATAGGAGGCTTTTGAGAATAAGTAGATGGTAATTATTAAGGTTTGGGAACGTTCATCTTCAAATTTCTACGCACTTTCCCAAGTAGTATTCTTTTTTTCTTTCTCTAGGCAAGTTGAAGGCTTTCTGTGAGCTTCTTTGCCCTATGCTAGGATGGTATTCTTATCATCCTTTTTTTCTTTTTTCTTTTTCTTCCACTATATAACATTTTGTTTATCCTTGAAGGGGAAAAAAGAGTCTGTCTGATGTAAGCTATTGATCCTGTTTTAACACTTGTGAACCGATCAATATAATACTGATTCTTCTTTGCTCTTCAGATCGTATATTTACTTCTGATTGGAAATTAATCCTAGTTTGAAGTAGTAATTTCTTTATAATATGAATTTGTATAGTGTCTTGATGTGTCACCTATCCTATTGCTTTCAGAACAACAGCGTAAGGTATTTACCTGGTTTTGGTCCTTCTGTTCCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCACGCTAGCAGATGTGAAGTCTATGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACGACAATTTCAGAGGTGCGATTTATGTGTGGGTCATTATAACTTGATATTGGATTAGTAGTATGATATTAAGCCATTGGAATTGTGCTGTTAGTTATTCTTGTCAAGTCAATATTACTTTGTAACTTTAGCCTTTACCTAATTTCCCTCATCAAAAGAATGGACCAAAAAGTTTGAAAATGCACAGTTTGGCTAATAAAAAATTTAATTATGATAAATAAGTAACCAGAAGATCAACTCATATAAAAAGTGTAGCTTTTAGGATGATATCTTTTTCCTTTTCTTTTCTCATAAGAGCAGCTGAGGTTACAATAAAATAAGTATTTTGTATTGTAATATTATGGATAACCTTGCACAATTCGTTATGGAGTCATCTCCAAATCCTTGGTACTCCATTGGCCAGCCTCCATGAGGGATATGTCCTGAGCTCGTCACCTTGACCATAGCATCGTAATACCTAAAAGAAAAAAAAAAAAGTCCAATAACTTCGTTTTTAAAAAAATATGTATAGGTATTTTTCTGGGTCGTATGTGCAATTGGTTAATTTTCTAACTCGTGAAGGATGGAGTAAATACAGATTTTAAAGTGGACACTTCACTAGAAACACCATTGTTAAGAGTCATCCCAAACATACTCTAAAAAGTGTTTTTCTTCATGTGCTGCATTTATGAACTCAGATGTATTTTTACTGTGCAGGAACTAATGGATGTTCCTTTATTTTTGAGTCTACACAACCTCTGTTCAACATTAAAGTGCACATCTCCATCCGCTGTAAATTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAACCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTTATTTGGGATATAATGCGTTGCTGGGTATGGCATTCTGAATTGTTCATTTCATTATATTCTTGAATGTCATTAGTTTATGTACTTGAATATCAAAAATATTATATACTTGAATGTGATTAGTTTATATATATTATAGCCTCGTGAGCAGATGTGGAACTGTCGAGCTTCCTTAATCCTTACATGTATCGAGGTCTCATCAAATTTTTTTTCATGTAGGTCAAAAACCACCCAGTAAAAGCTCAACCAGAAGATCAGCCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGTAAGTTCCATTTTGTAGTTCGACTGTTGGTTCGTGGTAACTTTTAGTTGCAATGTTTTTGCCCCTTGTTTTCTTTTGTCAAAAATGCTATATTATGTCCATGAACTAAAATTTGGCTTAGCATCAGAGATGACCTTGCACATTCAAACACCTAAAATGAATTGCCTGTGGCTGATGTACTTTTGCCAATTCTTATCTTCTTGGCAGGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAGCACATCTCTCTACTGGGTGCAGAGGCAGTAAATGGAGCTCTCAGCCAAACGGATTCTGAAGAGCCTAATGCAAAACGTCTCAAGGTAGAAGAAGATCCAGCTCCTAATCAGTAAATAGTTCTCTAATCTCATATTTTCTACCATGTTTCTAAGTTATCCAATATTTTCTTCTTGACCGGTCACCACATGAAATGCTTGTTGGTGGACTCTTGTGTAGAAGTAGCAAATTTTTGTTTAATGTTGAGGGAGGCTTTCTTTCAAAATATATCATTTTTGTTCATCTCTAGGATGTTTATATTTCTGTTGAGAGACGAAGATCACCAAGGAATGCACAAGTCTTTTCCTCTCCATCCCTATGATCACACGTTGCTGTACCATGCTGGTTCTGTTCTTATTTCATCTCCAATGGCAAGTTGAATAGTCAAACTAAGTATATAAACATTATATACTTGAATGTCATTAGCTTATTTTTATAATTTTGAGCCTTCTTCCCTTGAAAGGAAAGTTGCATAGTCAAAACTAAGTTGATAGCTTACAATTTAACAAGTCTTAGTTTTTAGAGCTTGGGATTGGAAAATTCAGATTGGTGTAAATGTCTGGATTATATAAAGATTGAGTCCCATGAGCTTTCAGTTTATGTTATGTAAGTGGAAATAGTAAATACATGGTTCAG

mRNA sequence

CCTCCAGAAACAGAAATCATGGTTCAGCCCCTAATCCATTCTTGTTGGTGTCTCACCACTCATTTTTCAGCCCCTAACTCGTGCTCCCAGGTCTTAGGAGCAAAAGAAGCGGAGACATTGGAGCTTTTTCAGGGTTGTGGATTTTTCTTTGACCCTTGAAACAAGCCAAGGAAAGGAAGAAGAAAGAATCCCAGTATCTGTTTTTTTCATGTGAGACTGAGAGCCGCAATAATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTTTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCTATTGCTGTTCTTAGGACATTTATATCAAAACGCAGGCAGGAGCATGAAGCAAAACTATCAAGAAGAGCCAAATCAGGGCAAAAGATTTCTGAAAATAATGGTTCTGAAACTGTTATGGAACAATCATCTAATGAATCTGCAACAAACAATGGAAAGTGCAATACAGATTGTGACGCTCATGAAAAAACATCACGTGAATGTGGAAGTCCGATGTTGTCAGAAGAACAGAAAGAGATCACAGAGGGAAAAGAACAAAGAGATCCCAAGCCTCTTAGAGTTCTCGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGGATTGATCACGTTGTGGCGCTAGACAACGACAAAGCATCGGTTGAAGCTTGTCAGAGAAACATCAAGTTTAATGGTTCAGTAGCGTGTGCAAAAGTGGAATCACATCTTACTGATGCTCGTGTATATATGCTCACCCACCCAAAAGAATTTGATATGGTTGATCTGGATCCTTATGGTTCACCTTCTATTTTCTTGGACTCTGCCGTTCAATCAGTTGTCGATGGTGGCATGCTGATGTGCACAGCAACTGATATGGCAGTGCTCTGTGGGGGAAATGGAGAAGTTTGCTATTCGAAATATGGTTCCTATCCTTTGAGAGGAAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCGAATAGATACAAGCGGTATATTGTTCCGGTGCTATCTGTGCAGATGGACTTCTATGTTCGGGTTTTTGTTCGTATTTATACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGGAGAAGCGTCTCCAAGAACAACAGCGTAAGGTATTTACCTGGTTTTGGTCCTTCTGTTCCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCACGCTAGCAGATGTGAAGTCTATGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACGACAATTTCAGAGGAACTAATGGATGTTCCTTTATTTTTGAGTCTACACAACCTCTGTTCAACATTAAAGTGCACATCTCCATCCGCTGTAAATTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAACCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTTATTTGGGATATAATGCGTTGCTGGGTCAAAAACCACCCAGTAAAAGCTCAACCAGAAGATCAGCCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAGCACATCTCTCTACTGGGTGCAGAGGCAGTAAATGGAGCTCTCAGCCAAACGGATTCTGAAGAGCCTAATGCAAAACGTCTCAAGGATGTTTATATTTCTGTTGAGAGACGAAGATCACCAAGGAATGCACAAGTCTTTTCCTCTCCATCCCTATGATCACACGTTGCTGTACCATGCTGGTTCTGTTCTTATTTCATCTCCAATGGCAAGTTGAATAGTCAAACTAAGTATATAAACATTATATACTTGAATGTCATTAGCTTATTTTTATAATTTTGAGCCTTCTTCCCTTGAAAGGAAAGTTGCATAGTCAAAACTAAGTTGATAGCTTACAATTTAACAAGTCTTAGTTTTTAGAGCTTGGGATTGGAAAATTCAGATTGGTGTAAATGTCTGGATTATATAAAGATTGAGTCCCATGAGCTTTCAGTTTATGTTATGTAAGTGGAAATAGTAAATACATGGTTCAG

Coding sequence (CDS)

ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTTTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCTATTGCTGTTCTTAGGACATTTATATCAAAACGCAGGCAGGAGCATGAAGCAAAACTATCAAGAAGAGCCAAATCAGGGCAAAAGATTTCTGAAAATAATGGTTCTGAAACTGTTATGGAACAATCATCTAATGAATCTGCAACAAACAATGGAAAGTGCAATACAGATTGTGACGCTCATGAAAAAACATCACGTGAATGTGGAAGTCCGATGTTGTCAGAAGAACAGAAAGAGATCACAGAGGGAAAAGAACAAAGAGATCCCAAGCCTCTTAGAGTTCTCGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGGATTGATCACGTTGTGGCGCTAGACAACGACAAAGCATCGGTTGAAGCTTGTCAGAGAAACATCAAGTTTAATGGTTCAGTAGCGTGTGCAAAAGTGGAATCACATCTTACTGATGCTCGTGTATATATGCTCACCCACCCAAAAGAATTTGATATGGTTGATCTGGATCCTTATGGTTCACCTTCTATTTTCTTGGACTCTGCCGTTCAATCAGTTGTCGATGGTGGCATGCTGATGTGCACAGCAACTGATATGGCAGTGCTCTGTGGGGGAAATGGAGAAGTTTGCTATTCGAAATATGGTTCCTATCCTTTGAGAGGAAAATATTGCCACGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCGAATAGATACAAGCGGTATATTGTTCCGGTGCTATCTGTGCAGATGGACTTCTATGTTCGGGTTTTTGTTCGTATTTATACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGGAGAAGCGTCTCCAAGAACAACAGCGTAAGGTATTTACCTGGTTTTGGTCCTTCTGTTCCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCACGCTAGCAGATGTGAAGTCTATGAAGGACTCTTATCCTGCATATGATCGTATATCTGCTGTATTGACGACAATTTCAGAGGAACTAATGGATGTTCCTTTATTTTTGAGTCTACACAACCTCTGTTCAACATTAAAGTGCACATCTCCATCCGCTGTAAATTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAACCCTTTAGGACTGAAATCAGATGCTCCCATGGATGTTATTTGGGATATAATGCGTTGCTGGGTCAAAAACCACCCAGTAAAAGCTCAACCAGAAGATCAGCCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAGCACATCTCTCTACTGGGTGCAGAGGCAGTAAATGGAGCTCTCAGCCAAACGGATTCTGAAGAGCCTAATGCAAAACGTCTCAAGGATGTTTATATTTCTGTTGAGAGACGAAGATCACCAAGGAATGCACAAGTCTTTTCCTCTCCATCCCTATGA

Protein sequence

MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSRRAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITEGKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVACAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSDCGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQITSKHISLLGAEAVNGALSQTDSEEPNAKRLKDVYISVERRRSPRNAQVFSSPSL
Homology
BLAST of Lcy09g003180 vs. ExPASy Swiss-Prot
Match: Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)

HSP 1 Score: 872.8 bits (2254), Expect = 2.1e-252
Identity = 434/564 (76.95%), Postives = 486/564 (86.17%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKR+QEHEAK S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R +   K+ E + SE   E++ +E+  NNG        HE  S E G   +S++  + TE
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMNNGD-------HEVAS-EDGPSSVSKDPAKTTE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EGI  VVALDND ASVEACQRNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPSIFLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS L  VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+KAQ  +QPGS+IL+KEP  E +F+R + SLSKAQAKK+ARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSE 565
           ITSKH+SL+G EAVNG LSQ   E
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE 556

BLAST of Lcy09g003180 vs. ExPASy Swiss-Prot
Match: Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)

HSP 1 Score: 850.5 bits (2196), Expect = 1.1e-245
Identity = 424/564 (75.18%), Postives = 484/564 (85.82%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLR FI KR+QEHEA LS+
Sbjct: 1   METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           RA+S  K+ E + SET  E++  E+  +NGK N +   HE T+++       +E  +   
Sbjct: 61  RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
              +R+ KP RVLEALSASGLRALRYAREVEGI  VVALDND ASVEACQRNIKFNG ++
Sbjct: 121 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PSIFLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLPG GP VPQ+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S L  VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L +TLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  + PGS+IL+KEP  +A+F+R V SLSKAQAKK+ARFLPNPE+HWGPK+RAGR 
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSE 565
           ITSKH+SLLG EAVNG L+    E
Sbjct: 541 ITSKHVSLLGHEAVNGHLNNNHKE 556

BLAST of Lcy09g003180 vs. ExPASy Swiss-Prot
Match: Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)

HSP 1 Score: 389.0 bits (998), Expect = 9.4e-107
Identity = 229/571 (40.11%), Postives = 331/571 (57.97%), Query Frame = 0

Query: 11  IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSRRAKSGQ-KIS 70
           + EG A+I+  + NEVF+N  Q  NRD++ AV+  F         A++   AK  Q K+ 
Sbjct: 58  VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIHLGAKGIQIKVP 117

Query: 71  ENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITEGKEQRDPKP 130
               SE +    S++     GK           +   G         EI E       + 
Sbjct: 118 GEKDSEKIAVDLSDQEEETAGKNENLAPGDWPRTAAVG---------EICE-------EG 177

Query: 131 LRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVACAKVESHLT 190
           LRVLE L+ASGLR++R+A EV G+  VVA D    +VE   RN++ NG      V+ +  
Sbjct: 178 LRVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQA 237

Query: 191 DARVYMLTH---PKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGE 250
           DAR+ M  H   P+ FD++DLDPYGSP+ FLD+AVQ+V DGG+L  T TDMAVL G +GE
Sbjct: 238 DARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGE 297

Query: 251 VCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTS 310
            CYSKYG+  L+ + CHEMALRI+L  ++ HAN Y+RYIVP+LS+  DFY+RVFVR++T 
Sbjct: 298 TCYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVFTG 357

Query: 311 ASAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSVRYLPGFGPSVPQECSDCGKKF 370
            + +K++  K + V+QC GC +F+LQ++G+ S      +++    GP V  EC  CG++ 
Sbjct: 358 QAKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQRH 417

Query: 371 NMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNL 430
            +GGP+W+ PIHD ++V   L  V +    +    RI  VL+ ++EEL DVPL+ +L  L
Sbjct: 418 QLGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLDQL 477

Query: 431 CSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQ 490
            ST+ C +P  +  RSA+++AG+R+S +H     +K+DAP + +WDIMRCW K  PVK +
Sbjct: 478 SSTIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVKRE 537

Query: 491 --PEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQITS 550
              E  P   ILA EP L+ANF     +   ++ + + RF  NPE +WGP+ RA     +
Sbjct: 538 RLSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKA 597

Query: 551 KHISLLGAEAVNGALSQTDSEEP--NAKRLK 573
               L G   +     +  +E+P   A RLK
Sbjct: 598 ASEDLAGRRRLLQNKRKEPAEDPAQRAARLK 601

BLAST of Lcy09g003180 vs. ExPASy Swiss-Prot
Match: Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.1e-102
Identity = 216/537 (40.22%), Postives = 317/537 (59.03%), Query Frame = 0

Query: 9   TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSRRAKSGQ-K 68
           T + EG A+I   + NEVF+N  Q  NRD++ AV+  F         A++   AK  Q K
Sbjct: 55  TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIQLGAKGIQIK 114

Query: 69  I-SENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITEGKEQRD 128
           +  E +  + V++ S  E               EK   +    + S +Q   T    +  
Sbjct: 115 VPGEKDTQKVVVDLSEQE--------------EEKVELKESENLASGDQPR-TAAVGEIC 174

Query: 129 PKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVACAKVES 188
            + L VLE L+ASGLR++R+A EV G+  VVA D    +V+  +RN++ N       V+ 
Sbjct: 175 EEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQP 234

Query: 189 HLTDARVYMLTHPK---EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGG 248
              DAR+ M  H +    FD++DLDPYGSP+ FLD+AVQ+V +GG+L  T TDMAVL G 
Sbjct: 235 SQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGN 294

Query: 249 NGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRI 308
           +GE CYSKYG+  L+ + CHEMALRI+L  ++  AN Y+R++VP+LS+  DFYVRVFVR+
Sbjct: 295 SGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRV 354

Query: 309 YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR-SVSKNNSVRYLPGFGPSVPQECSDCG 368
           +T  + +K +  K + V+QC GC +FHLQ++G+ S   +   ++    GP V  EC  CG
Sbjct: 355 FTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCG 414

Query: 369 KKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSL 428
           ++  +GGP+W+ PIHD ++V   L  V +    +   +RI  VL+ I+EEL DVPL+ +L
Sbjct: 415 QRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTL 474

Query: 429 HNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPV 488
             L ST+ C +PS +  RSA+++A +R+S +H     +K+DAP   +WDIMRCW K  PV
Sbjct: 475 DQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPV 534

Query: 489 KAQ--PEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRA 538
           K +   E  P   IL+ EP L+ANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 535 KRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRA 565

BLAST of Lcy09g003180 vs. ExPASy Swiss-Prot
Match: Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.2e-99
Identity = 216/540 (40.00%), Postives = 303/540 (56.11%), Query Frame = 0

Query: 10  IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSRRAKSGQKIS 69
           +I+E  AEI+  +   VF+N  Q  NRD+SIA L  +  +  +E   K  ++ +   K  
Sbjct: 19  VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVKEQ 78

Query: 70  ENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITEGKEQRDPKP 129
           E+                            EKT+     P + E      +G        
Sbjct: 79  ED----------------------------EKTTPVPEDPPVYEAGTRYEDG-------- 138

Query: 130 LRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVACAKVESHLT 189
           LR+LEAL+A+GLR++RYA+E+ G+  +VA D  + +V +   NI+ N      +      
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198

Query: 190 DARVYMLTHP-KEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVC 249
              +Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G   E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 258

Query: 250 YSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSAS 309
           Y KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+  DFY+R+FVR+Y   +
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVGQA 318

Query: 310 AMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNN-------SVRYLPGFGPSVPQECSDC 369
             K +  K S++YQCTGC++F LQ +G  ++K N        +++    GP+V  +C  C
Sbjct: 319 QCKLSMSKQSWIYQCTGCETFTLQPLG--ITKPNPTAGNPQQLKFGIPTGPAVNSQCEHC 378

Query: 370 GKKFNMGGPIWSAPIHDQEWVTSTLADV-KSMKDSYPAYDRISAVLTTISEELMDVPLFL 429
           G + ++GGPIWSAPIH+ E+V   L  V ++   S     RI  VL+ + EEL DVPL+ 
Sbjct: 379 GHRHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPLYY 438

Query: 430 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 489
           +   LC  LK      + FRSA+++AGYR+S +H +   LK++AP  V+WDI+R W K H
Sbjct: 439 TPDKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSKRH 498

Query: 490 PVKAQP--EDQPGSIILAKEPVLEANFARAVASLS-KAQAKKIARFLPNPERHWGPKLRA 538
           PV  +      P + IL+KE      F       + K++   ++RF  NP  HWGP  RA
Sbjct: 499 PVNPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGTRA 518

BLAST of Lcy09g003180 vs. ExPASy TrEMBL
Match: A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 540/572 (94.41%), Postives = 556/572 (97.20%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KR+QEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R+KSGQK+SENNGSET  E++ NESATN+ KC+TDCDAHEK S ECGSPMLSEEQKEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQR PKP+RVLEALSASGLRALRYAREVEG+  VVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSK 572

BLAST of Lcy09g003180 vs. ExPASy TrEMBL
Match: A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 554/572 (96.85%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KR+QEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R+KSGQK+SENNGSET  E++ N SATN  KC+TDCDAHEK S ECGSPMLSEEQKEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQR PKP+RVLEALSASGLRALRYAREVEG+  VVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPK 572

BLAST of Lcy09g003180 vs. ExPASy TrEMBL
Match: A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 540/572 (94.41%), Postives = 553/572 (96.68%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KR+QEHEAKLS+
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R+KSGQK+SENNGSET  E++ N SATN  KC+TDCDAHEK S ECGSPMLSEEQKEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQR PKP+RVLEALSASGLRALRYAREVEG+  VVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPK 572

BLAST of Lcy09g003180 vs. ExPASy TrEMBL
Match: A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 535/572 (93.53%), Postives = 553/572 (96.68%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR FISKR+QEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           RAKS QKISENNGSE VME++SNESATN+ KCNTDCDAHEK S ECGSP+LSEEQKE   
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+RD KP+RVLEALSASGLRALRYAREVEGI  VVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWV+S LADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGA+AVNGALS  DSEEPNAKR K
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSK 572

BLAST of Lcy09g003180 vs. ExPASy TrEMBL
Match: A0A6J1JNF2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487439 PE=3 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.2e-310
Identity = 536/572 (93.71%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR FISKR+QEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           RAKS QKISENNGSE VME++SNESATN+ KCNTDCDA EK S ECGSPML E QKE  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+RD KP+RVLEALSASGLRALRYAREVEGI  VVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS  DSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSK 572

BLAST of Lcy09g003180 vs. NCBI nr
Match: XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 548/572 (95.80%), Postives = 561/572 (98.08%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR FI+KR+QEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           RAKSGQK++ENNGSETVME++SNESATN+ KC+TDC AHEKTS ECGSPMLSEEQKE+ E
Sbjct: 61  RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQRDPKP+RVLEALSASGLRALRYAREVEGI  VVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPK 572

BLAST of Lcy09g003180 vs. NCBI nr
Match: XP_011656015.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN52527.1 hypothetical protein Csa_008279 [Cucumis sativus])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 540/572 (94.41%), Postives = 556/572 (97.20%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KR+QEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R+KSGQK+SENNGSET  E++ NESATN+ KC+TDCDAHEK S ECGSPMLSEEQKEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQR PKP+RVLEALSASGLRALRYAREVEG+  VVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSK 572

BLAST of Lcy09g003180 vs. NCBI nr
Match: XP_008459398.1 (PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 554/572 (96.85%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KR+QEHEAKLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R+KSGQK+SENNGSET  E++ N SATN  KC+TDCDAHEK S ECGSPMLSEEQKEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQR PKP+RVLEALSASGLRALRYAREVEG+  VVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPK 572

BLAST of Lcy09g003180 vs. NCBI nr
Match: KAA0039424.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa] >TYK00612.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 540/572 (94.41%), Postives = 553/572 (96.68%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KR+QEHEAKLS+
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R+KSGQK+SENNGSET  E++ N SATN  KC+TDCDAHEK S ECGSPMLSEEQKEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKEQR PKP+RVLEALSASGLRALRYAREVEG+  VVALDNDKASVEACQRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGAEAVNGALS TDSEEPNAKR K
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPK 572

BLAST of Lcy09g003180 vs. NCBI nr
Match: KAG6602539.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 536/572 (93.71%), Postives = 553/572 (96.68%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR FISKR+QEHE K+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           RAKS QKISENNGSE VME++SNESATN+ KCNTDCDAHEK S ECGSP+L EEQKE  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
           GKE+RD KP+RVLEALSASGLRALRYAREVEGI  VVALDNDKASVEAC+RNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTS LADVKSMKD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSEEPNAKRLK 573
           ITSKHISLLGA+AVNGALS  DSEEPNAKR K
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSK 572

BLAST of Lcy09g003180 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 872.8 bits (2254), Expect = 1.5e-253
Identity = 434/564 (76.95%), Postives = 486/564 (86.17%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKR+QEHEAK S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           R +   K+ E + SE   E++ +E+  NNG        HE  S E G   +S++  + TE
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMNNGD-------HEVAS-EDGPSSVSKDPAKTTE 120

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EGI  VVALDND ASVEACQRNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPSIFLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS L  VKSMKD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLC+TLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+KAQ  +QPGS+IL+KEP  E +F+R + SLSKAQAKK+ARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSQTDSE 565
           ITSKH+SL+G EAVNG LSQ   E
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE 556

BLAST of Lcy09g003180 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 850.5 bits (2196), Expect = 8.1e-247
Identity = 424/564 (75.18%), Postives = 484/564 (85.82%), Query Frame = 0

Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRTFISKRRQEHEAKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLR FI KR+QEHEA LS+
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158

Query: 61  RAKSGQKISENNGSETVMEQSSNESATNNGKCNTDCDAHEKTSRECGSPMLSEEQKEITE 120
           RA+S  K+ E + SET  E++  E+  +NGK N +   HE T+++       +E  +   
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 218

Query: 121 GKEQRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVA 180
              +R+ KP RVLEALSASGLRALRYAREVEGI  VVALDND ASVEACQRNIKFNG ++
Sbjct: 219 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PSIFLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398

Query: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360
           VR+YTSASAMKNTPLKLSYVYQC GCDSFHLQ +GRS+ KNNSVRYLPG GP VPQ+C+ 
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSTLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S L  VKSMKD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518

Query: 421 SLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L +TLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  + PGS+IL+KEP  +A+F+R V SLSKAQAKK+ARFLPNPE+HWGPK+RAGR 
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638

Query: 541 ITSKHISLLGAEAVNGALSQTDSE 565
           ITSKH+SLLG EAVNG L+    E
Sbjct: 639 ITSKHVSLLGHEAVNGHLNNNHKE 654

BLAST of Lcy09g003180 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 97.8 bits (242), Expect = 3.1e-20
Identity = 89/360 (24.72%), Postives = 150/360 (41.67%), Query Frame = 0

Query: 124 QRDPKPLRVLEALSASGLRALRYAREVEGIDHVVALDNDKASVEACQRNIKFNGSVACAK 183
           +R    LRVL+A+   G+R+LRY  E E  D V+A D +  +      N+        +K
Sbjct: 84  KRSNGSLRVLDAMCGCGIRSLRYLVEAEA-DFVMANDANDDNRRVITDNL--------SK 143

Query: 184 VESHLTDARVYMLTH-------------PKEFDMVDLDPYGSPSIFLDSAVQSVVDGGML 243
           VE    D R +++TH                FDM+D+D +GS S FL  A  ++  GG+L
Sbjct: 144 VERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLL 203

Query: 244 MCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLS 303
             T+TD     G       + YG++     + +E+ LR+L+      A     ++ P+ S
Sbjct: 204 YLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS 263

Query: 304 VQMDFYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGF 363
               ++  VF  +        +      +V  C  C   H            ++R+    
Sbjct: 264 -YYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSH------------TLRFDELG 323

Query: 364 GPSVPQECSDCGKKFNMGGPIWSAPIHDQEWVTSTLADVKS----MKDSYPAYDRISAVL 423
               P   +       + GP+W  P+HD  +VT  L   K      + +    D++ +++
Sbjct: 324 LMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIM 383

Query: 424 TTISEELMDVPLFLSLHNLCSTLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM 467
              S+  +  P +  +  + S  K  SP      SA++  GY  S +H+ P  LK+D PM
Sbjct: 384 IEESDPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRU72.1e-25276.95Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9LFU51.1e-24575.18Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... [more]
Q3TX089.4e-10740.11tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... [more]
Q9NXH91.1e-10240.22tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... [more]
Q9VK893.2e-9940.00Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... [more]
Match NameE-valueIdentityDescription
A0A0A0KVU40.0e+0094.41tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S3CA230.0e+0094.58tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A5A7T7H30.0e+0094.41tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1GSN60.0e+0093.53tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JNF22.2e-31093.71tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_038890035.10.0e+0095.80probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida][more]
XP_011656015.10.0e+0094.41probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN525... [more]
XP_008459398.10.0e+0094.58PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo][more]
KAA0039424.10.0e+0094.41putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa... [more]
KAG6602539.10.0e+0093.71putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
AT3G02320.11.5e-25376.95N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT5G15810.18.1e-24775.18N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G56330.13.1e-2024.72N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002905tRNA methyltransferase, Trm1TIGRFAMTIGR00308TIGR00308coord: 11..475
e-value: 4.0E-95
score: 317.3
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 12..474
e-value: 3.3E-119
score: 398.5
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 1..568
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 9..473
score: 139.073471
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 8..415
e-value: 4.0E-124
score: 415.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 94..126
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..126
NoneNo IPR availablePANTHERPTHR10631:SF12TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE 1-RELATEDcoord: 1..568
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 417..475
e-value: 6.3E-22
score: 79.0
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 8..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g003180.1Lcy09g003180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0003723 RNA binding