Lcy09g002980 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g002980
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING/U-box superfamily protein
LocationChr09: 2555931 .. 2557738 (-)
RNA-Seq ExpressionLcy09g002980
SyntenyLcy09g002980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATGGACTTCGTCAAAATCCCCAAATGTCATTTCCCCCAAAATTTCTATGGCATTTTTCTCCGTTCCTAAGCTTCATCTTCAACCTAGCATGCCTCTTCCTGATCAAACCCACTCCGATTCTCCTTCTCCGGTGCCGTCGATCCCATCTCTCCGCCGCGACGATCTCCCCAACCCCATGGATTTCAACCCTTTTGCCACCGTCCCCTCCATCGTCACATCTGCCGACGATTCCCTCCCTTTCGAGACCGACTGGTTTTCCGATACCGATTCCGACTCCGAACGCGATGTCAGTTGCTTCGTGACTGATCTTTTCGAGAATCGTCGTTTCACCGATCATTTGCACGCTCTCAGCGATCACGATCGAGGTCTGGATTCTGGGCCGGACCCATTTACGCATTCTGCTGACGACTTGGGCATTTCTGGATTTGAAGATAGCGGTGAGATTGAATCTAATTATATTGAGGAGCTGTGGTCGACGTTTGCTGTGGAATCTGATAGCCGGGTTTCGGAGGCGGAGGTGAGCATGCACGTTGCGGATTCGATTAATAGTGGCCTTCGGATTGTTGATGTGGATTCGGACTCGGATTCTGATAATGAAATGATTGGTGCTCTTCAACTCGTGACAGGGGATGATGGCGAGAATGATGTTAATTGGGTTGAAAGTCCGAATCCTCTCTCGGATAACTTCAGCTTTTGTTTTCAAGGTCAGAGGATTTCGTACGAGGATTTTGAATGGGAGGAGGTGGAGGAACGGGTGGACGAAAGGGAAATTTCTAGTGTAGTGGTGGATGGAGCTGAGGAATTGTCTCTTGCATCTGGTTTTTCCAATGAAGAAGAATCAGGAGAAGAGGCGGGTCGATGGGAAATTCTATTGGTAATGAACGATATTGGGAGGAATTCGAATTGGGAACGTGATAATGATGCTGAGGCGTACATAGCCGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAATGTTTTAAAGGGTAGTCCTCCAGCAGCTAAGAGTGCTGTGGAGAATCTTCCGCTGGTTGAGTTAAAAACAGAGAATATGCTGACAGAGGAAGTGGTTGTTTGTGCTGTTTGTAAGGATAAGTTTTCAATGGAGGAGAAGGTAAGGAAGCTCCCCTGTGGTCACTATTACCATGATGATTGCATTCTGCCATGGCTGCATATTCGAAACACATGTCCTGTTTGTCGGTTTGAGTTGCCAACCGATGATCCAGATTATGAGCGTAGGAAGAGTCAAAGGGCGAGTGGTGGCCTGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAAGATTAGTGCAATCTGTGAATAGCGTCACTTTTAGATGAAGAAAGTTGCTTACGATTTTGCATTTGTAAAGGTTTTGTGGTAAGTATTTCTTTAAAAAATTCTCCGCGTGGATCTACTAGCATTGCTGTCGATACGCTCCAATTATCTCCACAGATACCCATTGTGATTCAATGCTTGGTAAGTTTTAGTCACTAATAGTAGTGGGAAATTATATTGTCTCTTACCTTGTTGAATTCAATTTAGGAATTATAATGTTTGAGACAAGACATACTGATAATCACTTTACTACTAGTAGTACTGGGCTTTGAAATTGTATACTTGTGAAGTACGTTAAATTAGTATGTTTATATGCTTGAGTGTCTGTTGATAAACTTGTAAAAATAGGAAACCTCCTGGTATGAAATTTTGTGTGGCTCAATTGCTTGATTCTGGATCAAAATTATTCTGATAAGTTAGCTGAAT

mRNA sequence

CATGGACTTCGTCAAAATCCCCAAATGTCATTTCCCCCAAAATTTCTATGGCATTTTTCTCCGTTCCTAAGCTTCATCTTCAACCTAGCATGCCTCTTCCTGATCAAACCCACTCCGATTCTCCTTCTCCGGTGCCGTCGATCCCATCTCTCCGCCGCGACGATCTCCCCAACCCCATGGATTTCAACCCTTTTGCCACCGTCCCCTCCATCGTCACATCTGCCGACGATTCCCTCCCTTTCGAGACCGACTGGTTTTCCGATACCGATTCCGACTCCGAACGCGATGTCAGTTGCTTCGTGACTGATCTTTTCGAGAATCGTCGTTTCACCGATCATTTGCACGCTCTCAGCGATCACGATCGAGGTCTGGATTCTGGGCCGGACCCATTTACGCATTCTGCTGACGACTTGGGCATTTCTGGATTTGAAGATAGCGGTGAGATTGAATCTAATTATATTGAGGAGCTGTGGTCGACGTTTGCTGTGGAATCTGATAGCCGGGTTTCGGAGGCGGAGGTGAGCATGCACGTTGCGGATTCGATTAATAGTGGCCTTCGGATTGTTGATGTGGATTCGGACTCGGATTCTGATAATGAAATGATTGGTGCTCTTCAACTCGTGACAGGGGATGATGGCGAGAATGATGTTAATTGGGTTGAAAGTCCGAATCCTCTCTCGGATAACTTCAGCTTTTGTTTTCAAGGTCAGAGGATTTCGTACGAGGATTTTGAATGGGAGGAGGTGGAGGAACGGGTGGACGAAAGGGAAATTTCTAGTGTAGTGGTGGATGGAGCTGAGGAATTGTCTCTTGCATCTGGTTTTTCCAATGAAGAAGAATCAGGAGAAGAGGCGGGTCGATGGGAAATTCTATTGGTAATGAACGATATTGGGAGGAATTCGAATTGGGAACGTGATAATGATGCTGAGGCGTACATAGCCGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAATGTTTTAAAGGGTAGTCCTCCAGCAGCTAAGAGTGCTGTGGAGAATCTTCCGCTGGTTGAGTTAAAAACAGAGAATATGCTGACAGAGGAAGTGGTTGTTTGTGCTGTTTGTAAGGATAAGTTTTCAATGGAGGAGAAGGTAAGGAAGCTCCCCTGTGGTCACTATTACCATGATGATTGCATTCTGCCATGGCTGCATATTCGAAACACATGTCCTGTTTGTCGGTTTGAGTTGCCAACCGATGATCCAGATTATGAGCGTAGGAAGAGTCAAAGGGCGAGTGGTGGCCTGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAAGATTAGTGCAATCTGTGAATAGCGTCACTTTTAGATGAAGAAAGTTGCTTACGATTTTGCATTTGTAAAGGTTTTGTGGTAAGTATTTCTTTAAAAAATTCTCCGCGTGGATCTACTAGCATTGCTGTCGATACGCTCCAATTATCTCCACAGATACCCATTGTGATTCAATGCTTGGTAAGTTTTAGTCACTAATAGTAGTGGGAAATTATATTGTCTCTTACCTTGTTGAATTCAATTTAGGAATTATAATGTTTGAGACAAGACATACTGATAATCACTTTACTACTAGTAGTACTGGGCTTTGAAATTGTATACTTGTGAAGTACGTTAAATTAGTATGTTTATATGCTTGAGTGTCTGTTGATAAACTTGTAAAAATAGGAAACCTCCTGGTATGAAATTTTGTGTGGCTCAATTGCTTGATTCTGGATCAAAATTATTCTGATAAGTTAGCTGAAT

Coding sequence (CDS)

ATGGCATTTTTCTCCGTTCCTAAGCTTCATCTTCAACCTAGCATGCCTCTTCCTGATCAAACCCACTCCGATTCTCCTTCTCCGGTGCCGTCGATCCCATCTCTCCGCCGCGACGATCTCCCCAACCCCATGGATTTCAACCCTTTTGCCACCGTCCCCTCCATCGTCACATCTGCCGACGATTCCCTCCCTTTCGAGACCGACTGGTTTTCCGATACCGATTCCGACTCCGAACGCGATGTCAGTTGCTTCGTGACTGATCTTTTCGAGAATCGTCGTTTCACCGATCATTTGCACGCTCTCAGCGATCACGATCGAGGTCTGGATTCTGGGCCGGACCCATTTACGCATTCTGCTGACGACTTGGGCATTTCTGGATTTGAAGATAGCGGTGAGATTGAATCTAATTATATTGAGGAGCTGTGGTCGACGTTTGCTGTGGAATCTGATAGCCGGGTTTCGGAGGCGGAGGTGAGCATGCACGTTGCGGATTCGATTAATAGTGGCCTTCGGATTGTTGATGTGGATTCGGACTCGGATTCTGATAATGAAATGATTGGTGCTCTTCAACTCGTGACAGGGGATGATGGCGAGAATGATGTTAATTGGGTTGAAAGTCCGAATCCTCTCTCGGATAACTTCAGCTTTTGTTTTCAAGGTCAGAGGATTTCGTACGAGGATTTTGAATGGGAGGAGGTGGAGGAACGGGTGGACGAAAGGGAAATTTCTAGTGTAGTGGTGGATGGAGCTGAGGAATTGTCTCTTGCATCTGGTTTTTCCAATGAAGAAGAATCAGGAGAAGAGGCGGGTCGATGGGAAATTCTATTGGTAATGAACGATATTGGGAGGAATTCGAATTGGGAACGTGATAATGATGCTGAGGCGTACATAGCCGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAATGTTTTAAAGGGTAGTCCTCCAGCAGCTAAGAGTGCTGTGGAGAATCTTCCGCTGGTTGAGTTAAAAACAGAGAATATGCTGACAGAGGAAGTGGTTGTTTGTGCTGTTTGTAAGGATAAGTTTTCAATGGAGGAGAAGGTAAGGAAGCTCCCCTGTGGTCACTATTACCATGATGATTGCATTCTGCCATGGCTGCATATTCGAAACACATGTCCTGTTTGTCGGTTTGAGTTGCCAACCGATGATCCAGATTATGAGCGTAGGAAGAGTCAAAGGGCGAGTGGTGGCCTGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAA

Protein sequence

MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSADDSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSADDLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSDSDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDEREISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQDDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQTDLQVRYNFELIA
Homology
BLAST of Lcy09g002980 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 8.2e-28
Identity = 58/115 (50.43%), Postives = 79/115 (68.70%), Query Frame = 0

Query: 302 DYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKDK 361
           DY +    + L  Q  EN+    G+PPA+KSA++ LP V++ T++ML  E+  CAVC D+
Sbjct: 164 DYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKV-TKDMLKSEMNQCAVCMDE 223

Query: 362 FSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASG 417
           F     V+++PC H +H DC+LPWL + N+CPVCRFELPTDDPDYE R SQ + G
Sbjct: 224 FEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENR-SQGSQG 276

BLAST of Lcy09g002980 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.8e-27
Identity = 62/126 (49.21%), Postives = 79/126 (62.70%), Query Frame = 0

Query: 298 ADQDDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAV 357
           A+  DY      + L  Q  EN+    G+PPA+KSA+E LPLV + T++ L  E   CAV
Sbjct: 168 ANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNI-TKSNLNSEFNQCAV 227

Query: 358 CKDKFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYER--RKSQRAS 417
           C D F    + +++PC H YH DC+LPWL + N+CPVCR ELPTDDPDYER  R +Q  S
Sbjct: 228 CMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTS 287

Query: 418 GGLQTD 422
           GG   D
Sbjct: 288 GGNDGD 292

BLAST of Lcy09g002980 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.2e-25
Identity = 82/220 (37.27%), Postives = 115/220 (52.27%), Query Frame = 0

Query: 223 ISYED--FEWE-----EVEERVDEREISSVVVDGAEELSL-ASGFSNEEESGEEAGRWEI 282
           +SYED  F  E     E+++  DE E      +  EE +L  +   +EE+      R+ +
Sbjct: 100 LSYEDDFFRLELNSRNEIDDDEDEDEDDGDEEEEDEEENLTVNDEEDEEDDLRRRNRFPL 159

Query: 283 LLVMNDIGRNS--NWE------RDN------DAEAYIADQDDYM-YAAEYDTLFGQFVEN 342
               +  GRN   +W        DN      +++ Y  +  DY+  AA Y+ L     E 
Sbjct: 160 TTTQSRTGRNRILDWAEILMGIEDNSIEFRMESDRYAGNPADYIDDAAGYEALLQNLAEG 219

Query: 343 E----NVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKDKFSMEEKVRKLPCGH 402
           +       +G+PPAAKSA+E L   E+ +     E V+VCAVCKD   M E  +KLPCGH
Sbjct: 220 DGGGGGGRRGAPPAAKSAIEALETFEVSSSE--GEMVMVCAVCKDGMVMGETGKKLPCGH 279

Query: 403 YYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRAS 416
            YH DCI+PWL  RN+CPVCRF+L TDD +YE  + +R S
Sbjct: 280 CYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTS 317

BLAST of Lcy09g002980 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.7e-22
Identity = 48/108 (44.44%), Postives = 70/108 (64.81%), Query Frame = 0

Query: 302 DYMYAAEYDTLFGQFVE-----NENVLK-GSPPAAKSAVENLPLVELKTENMLTEEVVVC 361
           +++  + +D L  Q  +     N N+     PPA+KSA+E LPL+E+   ++L++    C
Sbjct: 142 EFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKSAIEALPLIEIDPTHLLSDSQSHC 201

Query: 362 AVCKDKFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDD 404
           AVCK+ F ++   R++PC H YH DCILPWL IRN+CPVCR ELP +D
Sbjct: 202 AVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRNSCPVCRHELPAED 249

BLAST of Lcy09g002980 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 6.3e-20
Identity = 43/78 (55.13%), Postives = 60/78 (76.92%), Query Frame = 0

Query: 325 GSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCILP 384
           G+PPA+KSA+E+LP VE+   ++ +E    CAVC + F  E + R++PC H +HDDCI+P
Sbjct: 171 GNPPASKSAIESLPRVEISDCHIGSE--ANCAVCTEIFETETEAREMPCKHLFHDDCIVP 230

Query: 385 WLHIRNTCPVCRFELPTD 403
           WL IRN+CPVCRFELP++
Sbjct: 231 WLSIRNSCPVCRFELPSE 246

BLAST of Lcy09g002980 vs. ExPASy TrEMBL
Match: A0A6J1JWL4 (uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.3e-221
Identity = 381/432 (88.19%), Postives = 407/432 (94.21%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEESGEEAGRWEILLVMN+I RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVRYNFELIA 433
           DL+VRYNFELIA
Sbjct: 421 DLRVRYNFELIA 432

BLAST of Lcy09g002980 vs. ExPASy TrEMBL
Match: A0A6J1FE59 (uncharacterized protein LOC111444905 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444905 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 5.6e-221
Identity = 380/432 (87.96%), Postives = 407/432 (94.21%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEE+GEEAGRWEILLVMNDI RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKS+RA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAGGGLQT 420

Query: 421 DLQVRYNFELIA 433
           DL+VRYNFELIA
Sbjct: 421 DLRVRYNFELIA 432

BLAST of Lcy09g002980 vs. ExPASy TrEMBL
Match: A0A6J1E557 (uncharacterized protein LOC111430034 OS=Cucurbita moschata OX=3662 GN=LOC111430034 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.2e-219
Identity = 382/432 (88.43%), Postives = 404/432 (93.52%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           MAFFS+PKLHLQP+MPLPDQ  SDSPSPVPSI S RRD+ PNPMDFNPFATVPSIV   D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL ALS+HD  LDSGPDPFTHSA+
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVESDSRVSE +VSM VADSINS LR+VDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
            +N +IGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQ QR SYEDFEWEEVEERVDER
Sbjct: 181 YENGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRNSYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           EISSVVV+GAEEL++ASGFSN+EESGEEAGRWEILLVM+DIG N+NWERDNDAEAY+ADQ
Sbjct: 241 EISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYAAEYDTLFGQFVENEN LKG PPAAKSA+ENLPLVELKTEN+LTEEVV+CAVCKD
Sbjct: 301 DDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKSQRASGG QT
Sbjct: 361 KFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQT 420

Query: 421 DLQVRYNFELIA 433
           DLQVRYNFELIA
Sbjct: 421 DLQVRYNFELIA 431

BLAST of Lcy09g002980 vs. ExPASy TrEMBL
Match: A0A6J1JYD4 (uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 9.9e-218
Identity = 375/428 (87.62%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEESGEEAGRWEILLVMN+I RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVRYNF 429
           DL+VR+ F
Sbjct: 421 DLRVRFPF 428

BLAST of Lcy09g002980 vs. ExPASy TrEMBL
Match: A0A6J1FKT6 (uncharacterized protein LOC111444905 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444905 PE=4 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.7e-217
Identity = 374/428 (87.38%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEE+GEEAGRWEILLVMNDI RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKS+RA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAGGGLQT 420

Query: 421 DLQVRYNF 429
           DL+VR+ F
Sbjct: 421 DLRVRFPF 428

BLAST of Lcy09g002980 vs. NCBI nr
Match: XP_022992755.1 (uncharacterized protein LOC111488995 isoform X4 [Cucurbita maxima])

HSP 1 Score: 777.3 bits (2006), Expect = 6.8e-221
Identity = 381/432 (88.19%), Postives = 407/432 (94.21%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEESGEEAGRWEILLVMN+I RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVRYNFELIA 433
           DL+VRYNFELIA
Sbjct: 421 DLRVRYNFELIA 432

BLAST of Lcy09g002980 vs. NCBI nr
Match: XP_022938801.1 (uncharacterized protein LOC111444905 isoform X3 [Cucurbita moschata])

HSP 1 Score: 776.5 bits (2004), Expect = 1.2e-220
Identity = 380/432 (87.96%), Postives = 407/432 (94.21%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEE+GEEAGRWEILLVMNDI RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKS+RA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAGGGLQT 420

Query: 421 DLQVRYNFELIA 433
           DL+VRYNFELIA
Sbjct: 421 DLRVRYNFELIA 432

BLAST of Lcy09g002980 vs. NCBI nr
Match: XP_022921923.1 (uncharacterized protein LOC111430034 [Cucurbita moschata])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 382/432 (88.43%), Postives = 404/432 (93.52%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           MAFFS+PKLHLQP+MPLPDQ  SDSPSPVPSI S RRD+ PNPMDFNPFATVPSIV   D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL ALS+HD  LDSGPDPFTHSA+
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVESDSRVSE +VSM VADSINS LR+VDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
            +N +IGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQ QR SYEDFEWEEVEERVDER
Sbjct: 181 YENGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRNSYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           EISSVVV+GAEEL++ASGFSN+EESGEEAGRWEILLVM+DIG N+NWERDNDAEAY+ADQ
Sbjct: 241 EISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYAAEYDTLFGQFVENEN LKG PPAAKSA+ENLPLVELKTEN+LTEEVV+CAVCKD
Sbjct: 301 DDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKSQRASGG QT
Sbjct: 361 KFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQT 420

Query: 421 DLQVRYNFELIA 433
           DLQVRYNFELIA
Sbjct: 421 DLQVRYNFELIA 431

BLAST of Lcy09g002980 vs. NCBI nr
Match: XP_023549550.1 (E3 ubiquitin ligase BIG BROTHER-related-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 377/432 (87.27%), Postives = 404/432 (93.52%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPVPS+PSLRRDDLPN MDFNPFA + SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFAAISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +V+M VADS  SGLRIVDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVNMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDI RN+NWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+L EE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLAEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKS+RA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAGGGLQT 420

Query: 421 DLQVRYNFELIA 433
           DL+VRYNFE IA
Sbjct: 421 DLRVRYNFEPIA 432

BLAST of Lcy09g002980 vs. NCBI nr
Match: KAG7016086.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 378/432 (87.50%), Postives = 404/432 (93.52%), Query Frame = 0

Query: 1   MAFFSVPKLHLQPSMPLPDQTHSDSPSPVPSIPSLRRDDLPNPMDFNPFATVPSIVTSAD 60
           M F S+PKL+LQP++ LPDQTHSDSPSPV S+PSLR DDLPN MDFNPFAT+ SIVTSAD
Sbjct: 24  MDFLSIPKLNLQPNVALPDQTHSDSPSPVLSVPSLRHDDLPNTMDFNPFATISSIVTSAD 83

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSGPDPFTHSAD 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDSGPDPFTHSA+
Sbjct: 84  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 143

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVADSINSGLRIVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESDSRVSE +VSM VADS  SGLRIVDVDSDS+
Sbjct: 144 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 203

Query: 181 SDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQGQRISYEDFEWEEVEERVDER 240
           SDN MIGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQGQRI YEDFEWEEVEERVDER
Sbjct: 204 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 263

Query: 241 EISSVVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDI RN+NWERDNDAEAYIADQ
Sbjct: 264 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIVRNTNWERDNDAEAYIADQ 323

Query: 301 DDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKD 360
           DDYMYA EYDTLFGQFVENEN LKGSPPAAKS+VENLPLVELKTEN+L EE+V CAVCKD
Sbjct: 324 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLAEELVACAVCKD 383

Query: 361 KFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWL+IRNTCPVCR+ELPTDDPDYERRKS+RA GGLQT
Sbjct: 384 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAGGGLQT 443

Query: 421 DLQVRYNFELIA 433
           DL+VRYNFELIA
Sbjct: 444 DLRVRYNFELIA 455

BLAST of Lcy09g002980 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-42
Identity = 120/337 (35.61%), Postives = 180/337 (53.41%), Query Frame = 0

Query: 109 DSGPDP-------FTHSADDLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEV--- 168
           DS PDP       F   + D+ ++ + +SG + +     +W  +  + D    E  +   
Sbjct: 69  DSEPDPNSCPIDFFDRDSSDVDVAEYLESGGLTAGDY-NIWGFYDPKEDEEEEEIVLGTS 128

Query: 169 --SMHVADSINSGLRIVDVDSDSDSDNEMIGALQLVTGDDGENDVNWVESPNPLSDNFSF 228
              +   DS   GLR+  +DSDSD ++ +   +   +  +  ND   VE    L   +  
Sbjct: 129 GSDLQPGDSGEQGLRVTGIDSDSDCEDGVFDFISEDSSGNRGNDSGRVEVGTGLPPVWDH 188

Query: 229 CFQGQRISYEDFEWEEVEERVDEREISSVVVDGAEELSLASGFSNEEESGEEAGRWEILL 288
            F G+     D EWEEV+  ++    S    D  EE  L+S   ++EE   E   W++LL
Sbjct: 189 LF-GEGTVLADEEWEEVQNAINWTAFSG-PEDEDEEDELSSLSRDDEEEDHELD-WQVLL 248

Query: 289 VMNDI--------GRNSNWERDNDAEAYI----ADQDDYMYAAEY--DTLFGQFVENENV 348
            +N++        G   N + D D + Y+     D+ D  ++  Y  D + GQ  ++E  
Sbjct: 249 TVNNVVNYIEQAEGIMLNPD-DIDPDYYLYLSGLDEFDENHSGHYDADAILGQMFDDETG 308

Query: 349 LKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCI 408
           ++G+PPAAKS +++LP+VEL  E  L +   VCAVCKD+  +EEKVR+LPC H+YH +CI
Sbjct: 309 IRGNPPAAKSVIQDLPVVELAVEE-LDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGECI 368

Query: 409 LPWLHIRNTCPVCRFELPTDDPDYERRK-SQRASGGL 419
           +PWL IRNTCPVCR+ELPTDD +YER K S+R   GL
Sbjct: 369 IPWLGIRNTCPVCRYELPTDDLEYERHKSSERGDTGL 399

BLAST of Lcy09g002980 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 6.4e-36
Identity = 106/296 (35.81%), Postives = 158/296 (53.38%), Query Frame = 0

Query: 148 ESDSRVSEAEVSMHVADSINSG--LRIVDVDSDSDSDNEMIGALQLVTGDDGENDVNWVE 207
           + D  V   +  +   D  +SG  +R++ V+S SD D+ +    +L   D  E DV +V 
Sbjct: 82  DDDFFVGRTDFGLEFRDIASSGNDIRLITVESGSDDDDGVENERELWGIDLNEEDV-YVN 141

Query: 208 SPNPLSDN----------FSFCFQGQRISYEDFEWEEVEER-----VDEREISS------ 267
             +   D+           S   + + ++ ++F+WEEV         DEREI S      
Sbjct: 142 DDDEYEDDDVSVTIPLCWDSLQLEDREVTADEFDWEEVGGGGGGGVDDEREIRSGFAQID 201

Query: 268 ----VVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYI-AD 327
                ++  +  +SL    + E   G     WE+LL   +     N++ DN  E YI  D
Sbjct: 202 MNDESLISASPIISLEGLVTRERAEGSGNLGWEVLL---NHTLEINFDVDN-RELYIGGD 261

Query: 328 QDDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCK 387
            DDY+   +YD LF QF + E  + G PP +KS + NLP+V L+ EN   +  +VCAVCK
Sbjct: 262 HDDYV--QDYDMLFEQFADAEVSVIGLPPTSKSFLNNLPVVLLEGEND-DDGGLVCAVCK 321

Query: 388 DKFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRAS 416
           D+ ++  K  +LPC H YH +CI+PWL +RNTCPVCR+ELPTDD +YE+RK+QR +
Sbjct: 322 DEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDDAEYEQRKTQRTT 369

BLAST of Lcy09g002980 vs. TAIR 10
Match: AT5G15820.1 (RING/U-box superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 3.5e-34
Identity = 81/188 (43.09%), Postives = 112/188 (59.57%), Query Frame = 0

Query: 233 VEERVDEREISSVVVDGAEELSLASGFSNEEESGEE----------AGRWEILLVMNDIG 292
           V + V E E  +  +   +E + AS    EEE  +E          +  WEILL  N   
Sbjct: 164 VVDEVLEWENFNNAIHLVQEPAYASMEGEEEEEEDEVVMEFAASIYSDAWEILLYDN--- 223

Query: 293 RNSNWERDNDAEAYIADQDDYMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVEL 352
             ++   D D E ++   D Y    +Y+ + GQ  +NE  +KG+PPA+KS V+ LP VEL
Sbjct: 224 MTNSAPMDLDVEVWLDSVDGYA-PMDYNAIIGQMFDNETGIKGTPPASKSVVDGLPDVEL 283

Query: 353 KTENMLTEEVVVCAVCKDKFSMEEKVRKLPCGHYYHDDCILPWLHIRNTCPVCRFELPTD 411
             E  L+   +VCA+CKD+   +EKV++LPC HYYH +CI+PWL IRNTCPVCR ELPTD
Sbjct: 284 TIEE-LSSVSIVCAICKDEVVFKEKVKRLPCKHYYHGECIIPWLGIRNTCPVCRHELPTD 343

BLAST of Lcy09g002980 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 4.5e-29
Identity = 128/402 (31.84%), Postives = 192/402 (47.76%), Query Frame = 0

Query: 52  VPSIVTSADDSLPFETDWFSDTD-SDSERDVSCFV------TDLFENRRFTDHLHALSDH 111
           +P  + S D  L     + S+TD S  +  VS  V       DL + R F   L     H
Sbjct: 42  LPHWIQSDDGDL-----YISETDFSSGDVSVSDLVFTTDDGLDLLDRRSFVMDLF----H 101

Query: 112 DRGLDSGPDPFTHSADDLGISGFEDSGEIESNYIEELWSTFAVESDSRVSEAEVSMHVAD 171
            R   S   P     D+ GI  FE+    E N   EL     +ES +   ++++ +++ +
Sbjct: 102 QRVEQSQVTPL----DNDGIDDFENYEMREDNV--ELDFGLEMESGNGFVDSDLVVNLNE 161

Query: 172 --------SINSGLRIVDVDSDSDSDNE--------MIGALQL-----VTGDDGENDVNW 231
                    I SG  IV V+S  DSD+E         I  + L        DD +ND   
Sbjct: 162 HVDGMRLLGIESGDVIVTVESCFDSDDEDDQEKEESEIWGIDLNEEDEYVNDDDDND--- 221

Query: 232 VESPNPLSDNFSFCFQGQR-----ISYEDFEWEEVEERVDEREISSVVVDGAE----ELS 291
            +     S     C+   +     I+ ED EWEEV+   DERE+ SV+ +  +     +S
Sbjct: 222 -DEEEDASVTIPLCWDSLQLEDLGINNEDCEWEEVDSD-DEREVLSVLAEADDNNSVSVS 281

Query: 292 LASGFSNE-----EESGEEAGRWEILLVMNDIGRNSNWERDNDAEAYIADQD-------D 351
           +A+  S E     E  G     WE+LL    +  N + + +++ E YI D D       D
Sbjct: 282 VAATISLEDLAISERRGSSNLGWEVLLNSRSLEFNLD-DAESNLELYIGDIDHEEEDYED 341

Query: 352 YMYAAEYDTLFGQFVENENVLKGSPPAAKSAVENLPLVELKTENML--TEEVVVCAVCKD 403
           Y++  EY+ LF   + +     G PPA+KS ++NL +  L  E+++   ++ V CAVCK+
Sbjct: 342 YLHTTEYEMLFEAEISSG---IGKPPASKSFIKNLKVSPLSNEDVMENDDDAVCCAVCKE 401

BLAST of Lcy09g002980 vs. TAIR 10
Match: AT5G64920.1 (COP1-interacting protein 8 )

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-26
Identity = 82/220 (37.27%), Postives = 115/220 (52.27%), Query Frame = 0

Query: 223 ISYED--FEWE-----EVEERVDEREISSVVVDGAEELSL-ASGFSNEEESGEEAGRWEI 282
           +SYED  F  E     E+++  DE E      +  EE +L  +   +EE+      R+ +
Sbjct: 100 LSYEDDFFRLELNSRNEIDDDEDEDEDDGDEEEEDEEENLTVNDEEDEEDDLRRRNRFPL 159

Query: 283 LLVMNDIGRNS--NWE------RDN------DAEAYIADQDDYM-YAAEYDTLFGQFVEN 342
               +  GRN   +W        DN      +++ Y  +  DY+  AA Y+ L     E 
Sbjct: 160 TTTQSRTGRNRILDWAEILMGIEDNSIEFRMESDRYAGNPADYIDDAAGYEALLQNLAEG 219

Query: 343 E----NVLKGSPPAAKSAVENLPLVELKTENMLTEEVVVCAVCKDKFSMEEKVRKLPCGH 402
           +       +G+PPAAKSA+E L   E+ +     E V+VCAVCKD   M E  +KLPCGH
Sbjct: 220 DGGGGGGRRGAPPAAKSAIEALETFEVSSSE--GEMVMVCAVCKDGMVMGETGKKLPCGH 279

Query: 403 YYHDDCILPWLHIRNTCPVCRFELPTDDPDYERRKSQRAS 416
            YH DCI+PWL  RN+CPVCRF+L TDD +YE  + +R S
Sbjct: 280 CYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTS 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPN78.2e-2850.43E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH301.8e-2749.21E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q9SPL22.2e-2537.27E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
O222836.7e-2244.44Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q940T56.3e-2055.13E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JWL43.3e-22188.19uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FE595.6e-22187.96uncharacterized protein LOC111444905 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1E5575.2e-21988.43uncharacterized protein LOC111430034 OS=Cucurbita moschata OX=3662 GN=LOC1114300... [more]
A0A6J1JYD49.9e-21887.62uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FKT61.7e-21787.38uncharacterized protein LOC111444905 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_022992755.16.8e-22188.19uncharacterized protein LOC111488995 isoform X4 [Cucurbita maxima][more]
XP_022938801.11.2e-22087.96uncharacterized protein LOC111444905 isoform X3 [Cucurbita moschata][more]
XP_022921923.11.1e-21888.43uncharacterized protein LOC111430034 [Cucurbita moschata][more]
XP_023549550.11.1e-21887.27E3 ubiquitin ligase BIG BROTHER-related-like isoform X4 [Cucurbita pepo subsp. p... [more]
KAG7016086.11.1e-21887.50E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G02340.11.6e-4235.61RING/U-box superfamily protein [more]
AT5G08139.16.4e-3635.81RING/U-box superfamily protein [more]
AT5G15820.13.5e-3443.09RING/U-box superfamily protein [more]
AT5G60820.14.5e-2931.84RING/U-box superfamily protein [more]
AT5G64920.11.6e-2637.27COP1-interacting protein 8 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 355..395
e-value: 8.6E-8
score: 41.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 355..396
e-value: 1.2E-11
score: 44.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 355..396
score: 12.607302
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 315..402
e-value: 5.3E-22
score: 79.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..30
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 113..416
NoneNo IPR availablePANTHERPTHR15710:SF108E3 UBIQUITIN-PROTEIN LIGASE CIP8-LIKE ISOFORM X1coord: 113..416
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 355..396
e-value: 1.41269E-23
score: 90.84
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 339..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g002980.1Lcy09g002980.1mRNA