Lcy09g001580 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g001580
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionProtein-serine/threonine phosphatase
LocationChr09: 1336761 .. 1338198 (+)
RNA-Seq ExpressionLcy09g001580
SyntenyLcy09g001580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGTCGCCGATCTCACTGCTTCCTCGTCGTCGTCGGCGCCGCCGCACAACGGACGGAAACGCCGCAGAGTGCATCATCTTCCCCGGCGGTGTCAAAATGCAGAGGAATCATGATGTTCATAAGACGGTTAAGTATGGAGTGACCTCTGTTTGCGGAAGAAGAAGAGAAATGGAGGATGCCGTTTCGGTTCATCCCTTTTTCGCTACTCTCAAAAATCAACCCCAAAATCCTCTGCACTTTTTTGGGGTCTTCGACGGGCATGGTTGCTCTCATGTAAGAACAAAAGCTCTGTTCTTTATTGAGAAATAACAGAGCAAAGAAGCAAAGAGGAAGACGATTTATCCTAGATACAGACATTGGAATTTGAAAAAAAAAAACTTCTTATAACTTGTCTTGGGACTTAAAATTTTGCCCTGTTTTAAGAAAATCCAGGCTCCCTCTGCTTCAGAGCGTTGTGATATCGTCTTTCAACGTTGCTCTGTTTTCTGCTCTTTCTTTTAGATGTTGATTTTTGGGTTTTCTTTTTGGTGTTTTTTAGGTGTCGATGAGCTGCGAGAATCGGATGCATGAAATAGTGAAGGAAGAGATCGAGAGCGAATTAGGAGAAACAGAGGATTGGAAGAAATTGATGCAACGAAGCTTTGCGCGTATGGATGACGAAGTGGAGGAGTACAGCCATGACATCAAACAGAAAGAAAGCTCTAATATTACTTGCAGATGCGAACTCCAGACTCCTCATCAATACGACACCGTCGGATCCACCGCTCTCGTTGCCATCGTCGCGCCCGACAAATTCATCGTCTCCAACTGCGGCGACTCCCGCGCCGTTCTTTGCCGGAAAAGTGCCGTCGTTCCCCTTTCCTCCGACCATAAGGTAGTCCTATAAATATTTGCACTTTGTGTATTAGAACACATGCATCAATGCCATCCATTATTGAACTCAAAAGGGACAAAGGCATTGTCAATTTTGATCCATATTGATAATCACTTATTGGATCTTGTGCTAATTTTCAAGCCAGATCGGCCAGACGAACTGTCCCGTATAGAATCTACGGGCGGTCGCGTGATATACTGGGATGGGGCGAGAGTGCTGGGAGTTTTAGCGACGTCGAGAGCGATAGGAGACGGTTATCTAAAACCGTACGTGATATCGGAGCCGGAGGTGGTGGAAATGGATCGGACGGCGGAGGATGAGTTCATAGTATTGGCGAGCGATGGGTTGTGGGACGTGGTTTCAAATGAAAGTGCGTGCGACGCAGTCCGAATGTGCATGCGGGCACAGCGGTCGACGTCGTCGTCGAGATATAGGGGCAGTAGCTCAGATAAAAGTTGCTCGGACGCGTCCATTGTGCTGACGAAGTTGGCAATGGCAAAGCATAGTTCAGATAACATTAGCGTGGTGGTGATAGATTTGAGAAGGGATCATCATCACTAG

mRNA sequence

ATGCTGTCGCCGATCTCACTGCTTCCTCGTCGTCGTCGGCGCCGCCGCACAACGGACGGAAACGCCGCAGAGTGCATCATCTTCCCCGGCGGTGTCAAAATGCAGAGGAATCATGATGTTCATAAGACGGTTAAGTATGGAGTGACCTCTGTTTGCGGAAGAAGAAGAGAAATGGAGGATGCCGTTTCGGTTCATCCCTTTTTCGCTACTCTCAAAAATCAACCCCAAAATCCTCTGCACTTTTTTGGGGTCTTCGACGGGCATGGTTGCTCTCATGTGTCGATGAGCTGCGAGAATCGGATGCATGAAATAGTGAAGGAAGAGATCGAGAGCGAATTAGGAGAAACAGAGGATTGGAAGAAATTGATGCAACGAAGCTTTGCGCGTATGGATGACGAAGTGGAGGAGTACAGCCATGACATCAAACAGAAAGAAAGCTCTAATATTACTTGCAGATGCGAACTCCAGACTCCTCATCAATACGACACCGTCGGATCCACCGCTCTCGTTGCCATCGTCGCGCCCGACAAATTCATCGTCTCCAACTGCGGCGACTCCCGCGCCGTTCTTTGCCGGAAAAGTGCCGTCGTTCCCCTTTCCTCCGACCATAAGCCAGATCGGCCAGACGAACTGTCCCGTATAGAATCTACGGGCGGTCGCGTGATATACTGGGATGGGGCGAGAGTGCTGGGAGTTTTAGCGACGTCGAGAGCGATAGGAGACGGTTATCTAAAACCGTACGTGATATCGGAGCCGGAGGTGGTGGAAATGGATCGGACGGCGGAGGATGAGTTCATAGTATTGGCGAGCGATGGGTTGTGGGACGTGGTTTCAAATGAAAGTGCGTGCGACGCAGTCCGAATGTGCATGCGGGCACAGCGGTCGACGTCGTCGTCGAGATATAGGGGCAGTAGCTCAGATAAAAGTTGCTCGGACGCGTCCATTGTGCTGACGAAGTTGGCAATGGCAAAGCATAGTTCAGATAACATTAGCGTGGTGGTGATAGATTTGAGAAGGGATCATCATCACTAG

Coding sequence (CDS)

ATGCTGTCGCCGATCTCACTGCTTCCTCGTCGTCGTCGGCGCCGCCGCACAACGGACGGAAACGCCGCAGAGTGCATCATCTTCCCCGGCGGTGTCAAAATGCAGAGGAATCATGATGTTCATAAGACGGTTAAGTATGGAGTGACCTCTGTTTGCGGAAGAAGAAGAGAAATGGAGGATGCCGTTTCGGTTCATCCCTTTTTCGCTACTCTCAAAAATCAACCCCAAAATCCTCTGCACTTTTTTGGGGTCTTCGACGGGCATGGTTGCTCTCATGTGTCGATGAGCTGCGAGAATCGGATGCATGAAATAGTGAAGGAAGAGATCGAGAGCGAATTAGGAGAAACAGAGGATTGGAAGAAATTGATGCAACGAAGCTTTGCGCGTATGGATGACGAAGTGGAGGAGTACAGCCATGACATCAAACAGAAAGAAAGCTCTAATATTACTTGCAGATGCGAACTCCAGACTCCTCATCAATACGACACCGTCGGATCCACCGCTCTCGTTGCCATCGTCGCGCCCGACAAATTCATCGTCTCCAACTGCGGCGACTCCCGCGCCGTTCTTTGCCGGAAAAGTGCCGTCGTTCCCCTTTCCTCCGACCATAAGCCAGATCGGCCAGACGAACTGTCCCGTATAGAATCTACGGGCGGTCGCGTGATATACTGGGATGGGGCGAGAGTGCTGGGAGTTTTAGCGACGTCGAGAGCGATAGGAGACGGTTATCTAAAACCGTACGTGATATCGGAGCCGGAGGTGGTGGAAATGGATCGGACGGCGGAGGATGAGTTCATAGTATTGGCGAGCGATGGGTTGTGGGACGTGGTTTCAAATGAAAGTGCGTGCGACGCAGTCCGAATGTGCATGCGGGCACAGCGGTCGACGTCGTCGTCGAGATATAGGGGCAGTAGCTCAGATAAAAGTTGCTCGGACGCGTCCATTGTGCTGACGAAGTTGGCAATGGCAAAGCATAGTTCAGATAACATTAGCGTGGTGGTGATAGATTTGAGAAGGGATCATCATCACTAG

Protein sequence

MLSPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRDHHH
Homology
BLAST of Lcy09g001580 vs. ExPASy Swiss-Prot
Match: Q9LNW3 (Protein phosphatase 2C 3 OS=Arabidopsis thaliana OX=3702 GN=AIP1 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.2e-99
Identity = 186/324 (57.41%), Postives = 231/324 (71.30%), Query Frame = 0

Query: 45  KYGVTSVCGRRREMEDAVSVHPFFATLKNQ-PQNPLHFFGVFDGHGCSHVSMSCENRMHE 104
           +YGV SVCGRRR+MEDAV++HP F   + +  +   H+FGV+DGHGCSHV+  C+ R+HE
Sbjct: 120 RYGVASVCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAARCKERLHE 179

Query: 105 IVKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCELQTPHQYDT 164
           +V+EE  S+  + E+WKK+M+RSF RMD EV  +   +      +  CRCELQTP   D 
Sbjct: 180 LVQEEALSD--KKEEWKKMMERSFTRMDKEVVRWGETV-----MSANCRCELQTP-DCDA 239

Query: 165 VGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIY 224
           VGSTA+V+++ P+K IV+NCGDSRAVLCR    VPLS+DHKPDRPDEL RI+  GGRVIY
Sbjct: 240 VGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPDELDRIQEAGGRVIY 299

Query: 225 WDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESAC 284
           WDGARVLGVLA SRAIGD YLKPYV SEPEV   DRT EDEF++LA+DGLWDVV+NE+AC
Sbjct: 300 WDGARVLGVLAMSRAIGDNYLKPYVTSEPEVTVTDRTEEDEFLILATDGLWDVVTNEAAC 359

Query: 285 DAVRMCM---------RAQRSTSSSR-------------------YRGSSSDKSCSDASI 340
             VRMC+         R +  T   R                    RG  +DK+C++AS+
Sbjct: 360 TMVRMCLNRKSGRGRRRGETQTPGRRSEEEGKEEEEKVVGSRKNGKRGEITDKACTEASV 419

BLAST of Lcy09g001580 vs. ExPASy Swiss-Prot
Match: Q9ZW21 (Probable protein phosphatase 2C 24 OS=Arabidopsis thaliana OX=3702 GN=At2g29380 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.6e-97
Identity = 179/304 (58.88%), Postives = 230/304 (75.66%), Query Frame = 0

Query: 36  RNHDVHKTVKYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSM 95
           +N DV  + +YGV+SVCGRRREMEDAV++HP F++ KN  + P H+FGV+DGHGCSHV+ 
Sbjct: 69  QNVDV-SSPRYGVSSVCGRRREMEDAVAIHPSFSSPKNS-EFPQHYFGVYDGHGCSHVAA 128

Query: 96  SCENRMHEIVKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCEL 155
            C  R+H++V+EE+ S++ + E+WK  M+RSF RMD EV  +   +         C+C+L
Sbjct: 129 RCRERLHKLVQEELSSDMEDEEEWKTTMERSFTRMDKEVVSWGDSV-----VTANCKCDL 188

Query: 156 QTPHQYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIE 215
           QTP   D+VGSTA+V+++ PDK +V+NCGDSRAVLCR    VPLS+DHKPDRPDEL RIE
Sbjct: 189 QTP-ACDSVGSTAVVSVITPDKIVVANCGDSRAVLCRNGKPVPLSTDHKPDRPDELDRIE 248

Query: 216 STGGRVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWD 275
             GGRVIYWD  RVLGVLA SRAIGD YLKPYV  EPEV   DR  +D+ ++LASDGLWD
Sbjct: 249 GAGGRVIYWDCPRVLGVLAMSRAIGDNYLKPYVSCEPEVTITDR-RDDDCLILASDGLWD 308

Query: 276 VVSNESACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVI 335
           VVSNE+AC   RMC+R       +    + SDK+C++AS++LTKLA+A++SSDN+SVVVI
Sbjct: 309 VVSNETACSVARMCLRGGGRRQDNE-DPAISDKACTEASVLLTKLALARNSSDNVSVVVI 362

Query: 336 DLRR 340
           DLRR
Sbjct: 369 DLRR 362

BLAST of Lcy09g001580 vs. ExPASy Swiss-Prot
Match: Q9FIF5 (Probable protein phosphatase 2C 78 OS=Arabidopsis thaliana OX=3702 GN=SAG113 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.5e-96
Identity = 194/360 (53.89%), Postives = 236/360 (65.56%), Query Frame = 0

Query: 8   LPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTV----------KYGVTSVCGRRRE 67
           L RRRRR  T   + +          +     +  TV          KYGV SVCGRRRE
Sbjct: 64  LQRRRRRESTVAASTSTVFYETAKEVVVLCESLSSTVVALPDPEAYPKYGVASVCGRRRE 123

Query: 68  MEDAVSVHPFFATLKNQ-PQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGET 127
           MEDAV+VHPFF+  + +      H+ GV+DGHGCSHV+M C  R+HE+V+EE E++    
Sbjct: 124 MEDAVAVHPFFSRHQTEYSSTGFHYCGVYDGHGCSHVAMKCRERLHELVREEFEAD---- 183

Query: 128 EDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAPD 187
            DW+K M RSF RMD EV   + D   K      CRCELQ P   D VGSTA+V+++ P+
Sbjct: 184 ADWEKSMARSFTRMDMEVVALNADGAAK------CRCELQRP-DCDAVGSTAVVSVLTPE 243

Query: 188 KFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATS 247
           K IV+NCGDSRAVLCR    + LSSDHKPDRPDEL RI++ GGRVIYWDG RVLGVLA S
Sbjct: 244 KIIVANCGDSRAVLCRNGKAIALSSDHKPDRPDELDRIQAAGGRVIYWDGPRVLGVLAMS 303

Query: 248 RAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMR----A 307
           RAIGD YLKPYVIS PEV   DR   D+F++LASDGLWDVVSNE+AC  VRMC+R     
Sbjct: 304 RAIGDNYLKPYVISRPEVTVTDRANGDDFLILASDGLWDVVSNETACSVVRMCLRGKVNG 363

Query: 308 QRSTSSSRYR------------GSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           Q S+S  R              G   DK+C +AS++LT+LA+A+ SSDN+SVVV+DLRRD
Sbjct: 364 QVSSSPEREMTGVGAGNVVVGGGDLPDKACEEASLLLTRLALARQSSDNVSVVVVDLRRD 412

BLAST of Lcy09g001580 vs. ExPASy Swiss-Prot
Match: P49598 (Protein phosphatase 2C 37 OS=Arabidopsis thaliana OX=3702 GN=PP2CA PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.2e-94
Identity = 177/298 (59.40%), Postives = 222/298 (74.50%), Query Frame = 0

Query: 45  KYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEI 104
           K G TSVCGRRR+MEDAVS+HP F  L+   +N  HF+GVFDGHGCSHV+  C  R+H+I
Sbjct: 104 KIGTTSVCGRRRDMEDAVSIHPSF--LQRNSENH-HFYGVFDGHGCSHVAEKCRERLHDI 163

Query: 105 VKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDI---KQKESSNITCRCELQTPHQY 164
           VK+E+  E+  +++W + M +SF +MD EV +   ++       S   +CRCELQ+P Q 
Sbjct: 164 VKKEV--EVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSP-QC 223

Query: 165 DTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRV 224
           D VGSTA+V++V P+K IVSNCGDSRAVLCR    +PLS DHKPDRPDEL RI+  GGRV
Sbjct: 224 DAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRV 283

Query: 225 IYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNES 284
           IYWDGARVLGVLA SRAIGD YLKPYVI +PEV   DRT EDE ++LASDGLWDVV NE+
Sbjct: 284 IYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNET 343

Query: 285 ACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRR 340
           AC   RMC+R   +   S     ++  +CSDA+++LTKLA+A+ SSDN+SVVV+DLR+
Sbjct: 344 ACGVARMCLRGAGAGDDS----DAAHNACSDAALLLTKLALARQSSDNVSVVVVDLRK 391

BLAST of Lcy09g001580 vs. ExPASy Swiss-Prot
Match: Q5N9N2 (Probable protein phosphatase 2C 9 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0846300 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.0e-90
Identity = 177/321 (55.14%), Postives = 219/321 (68.22%), Query Frame = 0

Query: 45  KYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEI 104
           +YGVT+VCGRRREMEDAVS+ P F     +     HF+GVFDGHGCSHV+ +C++RMHEI
Sbjct: 99  RYGVTAVCGRRREMEDAVSIRPDFLPASGK----FHFYGVFDGHGCSHVATTCQDRMHEI 158

Query: 105 VKEE-IESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCELQTPHQYDT 164
           V EE  +   GE   W+ +M++SFARMD EV    +    +      C CE QTP + D 
Sbjct: 159 VAEEHNKGASGEVAPWRDVMEKSFARMDGEV---GNRASTRSDDEPACPCEQQTPSRRDH 218

Query: 165 VGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIY 224
            GSTA+VA+V+P + +V+N GDSRAV+ R    V LS DHKPDRPDEL RIE+ GGRVIY
Sbjct: 219 AGSTAVVAVVSPTQVVVANAGDSRAVISRAGVPVALSVDHKPDRPDELERIEAAGGRVIY 278

Query: 225 WDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESAC 284
           WDGARVLGVLA SRAIGDGYLKPYV SEPEV   +RT +DE ++LASDGLWDVV+NE AC
Sbjct: 279 WDGARVLGVLAMSRAIGDGYLKPYVTSEPEVTVTERTDDDECLILASDGLWDVVTNEMAC 338

Query: 285 DAVRMCM----------RAQRSTSSSR---------------YRGSSSDKSCSDASIVLT 340
           + VR C           R     SS+                 +  SSDK+CSDA+++LT
Sbjct: 339 EVVRACFHNNGPPAPAARPSGVPSSAEAAETENGGAASVKGISKAESSDKACSDAAMLLT 398

BLAST of Lcy09g001580 vs. ExPASy TrEMBL
Match: A0A6J1JRY9 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111489250 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.5e-133
Identity = 249/345 (72.17%), Postives = 281/345 (81.45%), Query Frame = 0

Query: 3   SPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAV 62
           SP +    + R+RR    +   C       K+  + D  +T KYGVTSVCGRRREMED V
Sbjct: 131 SPSTPPSHQGRKRRGVHHSLRRC------KKVDNSVDGVETAKYGVTSVCGRRREMEDTV 190

Query: 63  SVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKL 122
           SVHP FAT +NQPQ PLHFFGVFDGHGCSHVS+ CENRMHEI+KEEIESELGE+ +WK L
Sbjct: 191 SVHPCFATHRNQPQIPLHFFGVFDGHGCSHVSVRCENRMHEILKEEIESELGESMEWKTL 250

Query: 123 MQRSFARMDDEVEEYSH-------DIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAP 182
           MQRSF RMD+EV+EY H       D   K  + ++CRC+LQTP QYDTVGSTALVAIV P
Sbjct: 251 MQRSFGRMDEEVKEYCHHDDDDDDDDDDKNKNTVSCRCKLQTPRQYDTVGSTALVAIVTP 310

Query: 183 DKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLAT 242
            + I+SNCGDSRAVLCRK++V+PLSSDHKPDRPDELSRIES GGRVIYWDGARVLGVLA 
Sbjct: 311 HQLIISNCGDSRAVLCRKTSVLPLSSDHKPDRPDELSRIESRGGRVIYWDGARVLGVLAM 370

Query: 243 SRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQRS 302
           SRAIGDGYLKPYVISEPEVVE+DRT EDEF++LA+DGLWDVVSNE+ACD VR CMRA  +
Sbjct: 371 SRAIGDGYLKPYVISEPEVVEVDRTGEDEFLILATDGLWDVVSNETACDVVRTCMRAPPA 430

Query: 303 TSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           ++SS    S SDK+CSDASIVLTKLAMAKHSSDNISVVVIDLRRD
Sbjct: 431 STSSL---SGSDKTCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 466

BLAST of Lcy09g001580 vs. ExPASy TrEMBL
Match: A0A6J1FFR0 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111445318 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.6e-133
Identity = 249/343 (72.59%), Postives = 280/343 (81.63%), Query Frame = 0

Query: 3   SPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAV 62
           SP +    + R+RR    +   C       K+  N D  +T KYGVTSVCGRRREMED V
Sbjct: 126 SPSTPPSHQGRKRRGVHHSLRRC------KKVDNNVDGVETAKYGVTSVCGRRREMEDTV 185

Query: 63  SVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKL 122
           SVHP FAT +NQPQ PLHFFGVFDGHGCSHVS+ CENRMHEI+KEEIESELGE+ +WK L
Sbjct: 186 SVHPCFATHRNQPQIPLHFFGVFDGHGCSHVSVRCENRMHEILKEEIESELGESMEWKTL 245

Query: 123 MQRSFARMDDEVEEYSH-----DIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAPDK 182
           MQRSF RMD+EV+EY H     D   K  S ++CRC+LQTP QY+TVGSTALVAIV P +
Sbjct: 246 MQRSFGRMDEEVKEYCHHDDGGDDDDKNKSTVSCRCKLQTPRQYETVGSTALVAIVTPHQ 305

Query: 183 FIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATSR 242
            I+SNCGDSRAVLCRK++V+PLSSDHKPDRPDELSRIES GGRVIYWDGARVLGVLA SR
Sbjct: 306 LIISNCGDSRAVLCRKTSVLPLSSDHKPDRPDELSRIESRGGRVIYWDGARVLGVLAMSR 365

Query: 243 AIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQRSTS 302
           AIGDGYLKPYVISEPEVV +DRT EDEF++LA+DGLWDVVSNE+ACD VR CMRA  +++
Sbjct: 366 AIGDGYLKPYVISEPEVVVVDRTGEDEFLILATDGLWDVVSNETACDVVRTCMRAPPAST 425

Query: 303 SSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           SS    S SDK+CSDASIVLTKLAMAKHSSDNISVVVIDLRRD
Sbjct: 426 SSL---SGSDKTCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 459

BLAST of Lcy09g001580 vs. ExPASy TrEMBL
Match: A0A6J1BVK4 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111006141 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.8e-125
Identity = 245/349 (70.20%), Postives = 273/349 (78.22%), Query Frame = 0

Query: 12  RRRRRTTDGNAAECIIFPG----GVKMQRNHDVHKTVKYGVTSVCGRRREMEDAVSVHPF 71
           R+RR   D       +FPG          N++    VKYGVTSVCG RR+MEDAVSVHPF
Sbjct: 9   RKRRGVHD-------VFPGRHCQNADDDENNNYVVKVKYGVTSVCGIRRDMEDAVSVHPF 68

Query: 72  FATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEE-IESELG-----ETEDWKK 131
           FA LK    +PLHFFGVFDGHGCSHVSMSCENRMHEIVKEE IE+ELG     + E+WK+
Sbjct: 69  FAALKT--DHPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEMIENELGGETVADQEEWKR 128

Query: 132 LMQRSFARMDDEVEEYSHDIKQKESSNIT-CRCELQTPHQYDTVGSTALVAIVAPDKFIV 191
           LMQRSFARMD+EV EY  D    +++  T CRC+L T HQYDTVGSTALVA++ P  FI+
Sbjct: 129 LMQRSFARMDEEVREYRRDTDSDDANQTTSCRCQLHTLHQYDTVGSTALVAVLTPHNFII 188

Query: 192 SNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATSRAIG 251
           SNCGDSRAVLCRK   +PLS+DHKPDRPDEL RIES+GGRVI WDG RVLGVLATSRAIG
Sbjct: 189 SNCGDSRAVLCRKKLAIPLSNDHKPDRPDELVRIESSGGRVINWDGPRVLGVLATSRAIG 248

Query: 252 DGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQR------ 311
           DG LKPYVISEPEVV MDRTAEDEF+VLA+DGLWDVVSN++AC+AVR CMRAQR      
Sbjct: 249 DGNLKPYVISEPEVVVMDRTAEDEFVVLATDGLWDVVSNDTACEAVRTCMRAQRDRPTSS 308

Query: 312 ---STSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
              S SS R  G S DK+CSDASIVLTKLAMA+HSSDNISVVVIDLRRD
Sbjct: 309 LAPSQSSPRGMGCSCDKTCSDASIVLTKLAMARHSSDNISVVVIDLRRD 348

BLAST of Lcy09g001580 vs. ExPASy TrEMBL
Match: A0A0A0KD79 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_6G008720 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 7.3e-123
Identity = 234/326 (71.78%), Postives = 267/326 (81.90%), Query Frame = 0

Query: 40  VHKTVKYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCEN 99
           V   VKYGVTSVCGRRREMED VSVH +F   KN PQ P+HFFGVFDGHGCSHVSMSC N
Sbjct: 67  VDDDVKYGVTSVCGRRREMEDMVSVHLYFTNEKNLPQIPIHFFGVFDGHGCSHVSMSCMN 126

Query: 100 RMHEIVKEEI-ESELGETEDWKKLMQRSFARMDDEV-EEYSHDIKQKE------------ 159
           RMHEIVKEEI E+EL ETE+WKK+M+RSF RMD+EV +EYS++IKQ++            
Sbjct: 127 RMHEIVKEEIDENELEETEEWKKIMKRSFRRMDEEVMKEYSNNIKQRDAAVAGSSSSSSS 186

Query: 160 SSNITCRCELQTPHQYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRK-SAVVPLSSDHK 219
           S NI+CRCELQT HQYDTVGSTAL+ ++ P K I++NCGDSRAVL RK + ++PLSSDHK
Sbjct: 187 SHNISCRCELQTSHQYDTVGSTALIVLLMPHKLIIANCGDSRAVLSRKTTGILPLSSDHK 246

Query: 220 PDRPDELSRIESTGGRVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDE 279
           PDRPDELSRIES GG VI+W+GARVLGVLA SRAIGD  LKPYVISEPEVV MDR  EDE
Sbjct: 247 PDRPDELSRIESGGGHVIHWEGARVLGVLAMSRAIGDSSLKPYVISEPEVVVMDRRMEDE 306

Query: 280 FIVLASDGLWDVVSNESACDAVRMCMRAQRSTS-------SSRYRGSSSDKSCSDASIVL 339
           FI+LA+DGLWDVV+NE+ACDAVR CMRAQR++S       SS   G  SDK+CSDASI+L
Sbjct: 307 FIILATDGLWDVVTNETACDAVRTCMRAQRASSSLSLLEESSGSMGGGSDKTCSDASILL 366

Query: 340 TKLAMAKHSSDNISVVVIDLRRDHHH 344
           TKLA+AKHSSDNIS+VVIDLR  HHH
Sbjct: 367 TKLAIAKHSSDNISIVVIDLRNTHHH 392

BLAST of Lcy09g001580 vs. ExPASy TrEMBL
Match: A0A5A7TJA0 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00840 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 3.2e-118
Identity = 225/316 (71.20%), Postives = 255/316 (80.70%), Query Frame = 0

Query: 40  VHKTVKYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCEN 99
           V   VKYGVTSVCGRRREMED VSVH +F   KN PQ P+HFFGVFDGHGCSHVSMSC N
Sbjct: 67  VDDDVKYGVTSVCGRRREMEDMVSVHLYFTNQKNLPQIPIHFFGVFDGHGCSHVSMSCMN 126

Query: 100 RMHEIVKEEI-ESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKE----------SSN 159
           RMHEIVKEEI E ELGETE+WKK+M+RSF RMD+EV EY++ IKQ++          S N
Sbjct: 127 RMHEIVKEEIDEKELGETEEWKKIMRRSFRRMDEEVMEYANKIKQRDAAVAGSSSSSSQN 186

Query: 160 ITCRCELQTPHQYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRK-SAVVPLSSDHKPDR 219
            +CRCELQTP +YDTVGSTALV ++ P K I++NCGDSRAVL RK + + PLSSDHKPDR
Sbjct: 187 NSCRCELQTPSRYDTVGSTALVLLLMPHKLIIANCGDSRAVLSRKTTGIFPLSSDHKPDR 246

Query: 220 PDELSRIESTGGRVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIV 279
           PDELSRIES GG VIYW+GARVLGVLA SRAIGD  LKPYVI EPEVV MDR  EDEFI+
Sbjct: 247 PDELSRIESGGGHVIYWEGARVLGVLAMSRAIGDSSLKPYVIPEPEVVIMDRRMEDEFII 306

Query: 280 LASDGLWDVVSNESACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSS 339
           LA+DGLWDVV+NE+ACD VR CM+AQ+ +  S   G  SDK+CSDASI+LTKLA+AK SS
Sbjct: 307 LATDGLWDVVTNETACDVVRACMQAQQPSVGS--MGGGSDKTCSDASILLTKLAIAKRSS 366

Query: 340 DNISVVVIDLRRDHHH 344
           DNIS+VVIDL   H H
Sbjct: 367 DNISIVVIDLGTHHQH 380

BLAST of Lcy09g001580 vs. NCBI nr
Match: XP_022993147.1 (probable protein phosphatase 2C 24 isoform X1 [Cucurbita maxima])

HSP 1 Score: 484.6 bits (1246), Expect = 7.2e-133
Identity = 249/345 (72.17%), Postives = 281/345 (81.45%), Query Frame = 0

Query: 3   SPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAV 62
           SP +    + R+RR    +   C       K+  + D  +T KYGVTSVCGRRREMED V
Sbjct: 131 SPSTPPSHQGRKRRGVHHSLRRC------KKVDNSVDGVETAKYGVTSVCGRRREMEDTV 190

Query: 63  SVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKL 122
           SVHP FAT +NQPQ PLHFFGVFDGHGCSHVS+ CENRMHEI+KEEIESELGE+ +WK L
Sbjct: 191 SVHPCFATHRNQPQIPLHFFGVFDGHGCSHVSVRCENRMHEILKEEIESELGESMEWKTL 250

Query: 123 MQRSFARMDDEVEEYSH-------DIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAP 182
           MQRSF RMD+EV+EY H       D   K  + ++CRC+LQTP QYDTVGSTALVAIV P
Sbjct: 251 MQRSFGRMDEEVKEYCHHDDDDDDDDDDKNKNTVSCRCKLQTPRQYDTVGSTALVAIVTP 310

Query: 183 DKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLAT 242
            + I+SNCGDSRAVLCRK++V+PLSSDHKPDRPDELSRIES GGRVIYWDGARVLGVLA 
Sbjct: 311 HQLIISNCGDSRAVLCRKTSVLPLSSDHKPDRPDELSRIESRGGRVIYWDGARVLGVLAM 370

Query: 243 SRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQRS 302
           SRAIGDGYLKPYVISEPEVVE+DRT EDEF++LA+DGLWDVVSNE+ACD VR CMRA  +
Sbjct: 371 SRAIGDGYLKPYVISEPEVVEVDRTGEDEFLILATDGLWDVVSNETACDVVRTCMRAPPA 430

Query: 303 TSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           ++SS    S SDK+CSDASIVLTKLAMAKHSSDNISVVVIDLRRD
Sbjct: 431 STSSL---SGSDKTCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 466

BLAST of Lcy09g001580 vs. NCBI nr
Match: XP_022939386.1 (probable protein phosphatase 2C 24 isoform X1 [Cucurbita moschata])

HSP 1 Score: 484.2 bits (1245), Expect = 9.4e-133
Identity = 249/343 (72.59%), Postives = 280/343 (81.63%), Query Frame = 0

Query: 3   SPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAV 62
           SP +    + R+RR    +   C       K+  N D  +T KYGVTSVCGRRREMED V
Sbjct: 126 SPSTPPSHQGRKRRGVHHSLRRC------KKVDNNVDGVETAKYGVTSVCGRRREMEDTV 185

Query: 63  SVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKL 122
           SVHP FAT +NQPQ PLHFFGVFDGHGCSHVS+ CENRMHEI+KEEIESELGE+ +WK L
Sbjct: 186 SVHPCFATHRNQPQIPLHFFGVFDGHGCSHVSVRCENRMHEILKEEIESELGESMEWKTL 245

Query: 123 MQRSFARMDDEVEEYSH-----DIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAPDK 182
           MQRSF RMD+EV+EY H     D   K  S ++CRC+LQTP QY+TVGSTALVAIV P +
Sbjct: 246 MQRSFGRMDEEVKEYCHHDDGGDDDDKNKSTVSCRCKLQTPRQYETVGSTALVAIVTPHQ 305

Query: 183 FIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATSR 242
            I+SNCGDSRAVLCRK++V+PLSSDHKPDRPDELSRIES GGRVIYWDGARVLGVLA SR
Sbjct: 306 LIISNCGDSRAVLCRKTSVLPLSSDHKPDRPDELSRIESRGGRVIYWDGARVLGVLAMSR 365

Query: 243 AIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQRSTS 302
           AIGDGYLKPYVISEPEVV +DRT EDEF++LA+DGLWDVVSNE+ACD VR CMRA  +++
Sbjct: 366 AIGDGYLKPYVISEPEVVVVDRTGEDEFLILATDGLWDVVSNETACDVVRTCMRAPPAST 425

Query: 303 SSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           SS    S SDK+CSDASIVLTKLAMAKHSSDNISVVVIDLRRD
Sbjct: 426 SSL---SGSDKTCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 459

BLAST of Lcy09g001580 vs. NCBI nr
Match: XP_023551136.1 (probable protein phosphatase 2C 24 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 480.7 bits (1236), Expect = 1.0e-131
Identity = 246/350 (70.29%), Postives = 281/350 (80.29%), Query Frame = 0

Query: 3   SPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAV 62
           SP +    + R+RR    +   C       K+  N D  +T KYGVTSVCGRRREMED V
Sbjct: 85  SPSTPPSHQGRKRRGVHHSLRRC------KKVDNNVDGVETAKYGVTSVCGRRREMEDTV 144

Query: 63  SVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKL 122
           S+HP FAT +NQPQ PLHFFGVFDGHGCSHVS+ CENRMHE++KEEIESELGE+ +WK L
Sbjct: 145 SLHPCFATYRNQPQIPLHFFGVFDGHGCSHVSVRCENRMHELLKEEIESELGESMEWKTL 204

Query: 123 MQRSFARMDDEVEEYSH------------DIKQKESSNITCRCELQTPHQYDTVGSTALV 182
           MQRSF RMD+EV+E+ H            D   +  + ++CRC+LQTP QYDTVGSTALV
Sbjct: 205 MQRSFGRMDEEVKEFCHHDEDDGDEDEDEDEDDRNKNTVSCRCKLQTPRQYDTVGSTALV 264

Query: 183 AIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVL 242
           AIV P + I+SNCGDSRAVLCRK++V+PLSSDHKPDRPDELSRIES GGRVIYWDGARVL
Sbjct: 265 AIVTPHQLIISNCGDSRAVLCRKTSVLPLSSDHKPDRPDELSRIESRGGRVIYWDGARVL 324

Query: 243 GVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCM 302
           GVLA SRAIGDGYLKPYVISEPEVVE+DRT EDEF++LA+DGLWDVVSNE+ACD VR CM
Sbjct: 325 GVLAMSRAIGDGYLKPYVISEPEVVEVDRTGEDEFLILATDGLWDVVSNETACDVVRTCM 384

Query: 303 RAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           RA  +++SS    S SDK+CSDASIVLTKLAMAKHSSDNISVVVIDLRRD
Sbjct: 385 RAPPASTSSL---SGSDKTCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 425

BLAST of Lcy09g001580 vs. NCBI nr
Match: KAG7015982.1 (putative protein phosphatase 2C 24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 477.6 bits (1228), Expect = 8.8e-131
Identity = 249/361 (68.98%), Postives = 280/361 (77.56%), Query Frame = 0

Query: 3   SPISLLPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTVKYGVTSVCGRRREMEDAV 62
           SP +    + R+RR    +   C       K+  N D  +T KYGVTSVCGRRREMED V
Sbjct: 46  SPSTPPSHQGRKRRGVHHSLRRC------KKVDNNVDGVETAKYGVTSVCGRRREMEDTV 105

Query: 63  SVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGETEDWKKL 122
           SVHP FAT +NQPQ PLHFFGVFDGHGCSHVS+ CENRMHEI+KEEIESELGE+ +WK L
Sbjct: 106 SVHPCFATHRNQPQIPLHFFGVFDGHGCSHVSVRCENRMHEILKEEIESELGESMEWKTL 165

Query: 123 MQRSFARMDDEVEEYSH-----------------------DIKQKESSNITCRCELQTPH 182
           MQRSF RMD+EV+EY H                       D   K  + ++CRC+LQTP 
Sbjct: 166 MQRSFGRMDEEVKEYCHHDDDEDDGDDEDDEDDEDDEDDEDDDDKNKNTVSCRCKLQTPR 225

Query: 183 QYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGG 242
           QYDTVGSTALVAIV P + I+SNCGDSRAVLCRK++V+PLSSDHKPDRPDELSRIES GG
Sbjct: 226 QYDTVGSTALVAIVTPHQLIISNCGDSRAVLCRKTSVLPLSSDHKPDRPDELSRIESRGG 285

Query: 243 RVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSN 302
           RVIYWDGARVLGVLA SRAIGDGYLKPYVISEPEVV +DRT EDEF++LA+DGLWDVVSN
Sbjct: 286 RVIYWDGARVLGVLAMSRAIGDGYLKPYVISEPEVVVVDRTGEDEFLILATDGLWDVVSN 345

Query: 303 ESACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRR 341
           E+ACD VR CMRA  +++SS    S SDK+CSDASIVLTKLAMAKHSSDNISVVVIDLRR
Sbjct: 346 ETACDVVRTCMRAPPASTSSL---SGSDKTCSDASIVLTKLAMAKHSSDNISVVVIDLRR 397

BLAST of Lcy09g001580 vs. NCBI nr
Match: XP_022133601.1 (protein phosphatase 2C 3-like [Momordica charantia])

HSP 1 Score: 456.8 bits (1174), Expect = 1.6e-124
Identity = 245/349 (70.20%), Postives = 273/349 (78.22%), Query Frame = 0

Query: 12  RRRRRTTDGNAAECIIFPG----GVKMQRNHDVHKTVKYGVTSVCGRRREMEDAVSVHPF 71
           R+RR   D       +FPG          N++    VKYGVTSVCG RR+MEDAVSVHPF
Sbjct: 9   RKRRGVHD-------VFPGRHCQNADDDENNNYVVKVKYGVTSVCGIRRDMEDAVSVHPF 68

Query: 72  FATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEE-IESELG-----ETEDWKK 131
           FA LK    +PLHFFGVFDGHGCSHVSMSCENRMHEIVKEE IE+ELG     + E+WK+
Sbjct: 69  FAALKT--DHPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEMIENELGGETVADQEEWKR 128

Query: 132 LMQRSFARMDDEVEEYSHDIKQKESSNIT-CRCELQTPHQYDTVGSTALVAIVAPDKFIV 191
           LMQRSFARMD+EV EY  D    +++  T CRC+L T HQYDTVGSTALVA++ P  FI+
Sbjct: 129 LMQRSFARMDEEVREYRRDTDSDDANQTTSCRCQLHTLHQYDTVGSTALVAVLTPHNFII 188

Query: 192 SNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATSRAIG 251
           SNCGDSRAVLCRK   +PLS+DHKPDRPDEL RIES+GGRVI WDG RVLGVLATSRAIG
Sbjct: 189 SNCGDSRAVLCRKKLAIPLSNDHKPDRPDELVRIESSGGRVINWDGPRVLGVLATSRAIG 248

Query: 252 DGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMRAQR------ 311
           DG LKPYVISEPEVV MDRTAEDEF+VLA+DGLWDVVSN++AC+AVR CMRAQR      
Sbjct: 249 DGNLKPYVISEPEVVVMDRTAEDEFVVLATDGLWDVVSNDTACEAVRTCMRAQRDRPTSS 308

Query: 312 ---STSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
              S SS R  G S DK+CSDASIVLTKLAMA+HSSDNISVVVIDLRRD
Sbjct: 309 LAPSQSSPRGMGCSCDKTCSDASIVLTKLAMARHSSDNISVVVIDLRRD 348

BLAST of Lcy09g001580 vs. TAIR 10
Match: AT1G07430.1 (highly ABA-induced PP2C gene 2 )

HSP 1 Score: 361.7 bits (927), Expect = 6.6e-100
Identity = 186/324 (57.41%), Postives = 231/324 (71.30%), Query Frame = 0

Query: 45  KYGVTSVCGRRREMEDAVSVHPFFATLKNQ-PQNPLHFFGVFDGHGCSHVSMSCENRMHE 104
           +YGV SVCGRRR+MEDAV++HP F   + +  +   H+FGV+DGHGCSHV+  C+ R+HE
Sbjct: 120 RYGVASVCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAARCKERLHE 179

Query: 105 IVKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCELQTPHQYDT 164
           +V+EE  S+  + E+WKK+M+RSF RMD EV  +   +      +  CRCELQTP   D 
Sbjct: 180 LVQEEALSD--KKEEWKKMMERSFTRMDKEVVRWGETV-----MSANCRCELQTP-DCDA 239

Query: 165 VGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIY 224
           VGSTA+V+++ P+K IV+NCGDSRAVLCR    VPLS+DHKPDRPDEL RI+  GGRVIY
Sbjct: 240 VGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPDELDRIQEAGGRVIY 299

Query: 225 WDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESAC 284
           WDGARVLGVLA SRAIGD YLKPYV SEPEV   DRT EDEF++LA+DGLWDVV+NE+AC
Sbjct: 300 WDGARVLGVLAMSRAIGDNYLKPYVTSEPEVTVTDRTEEDEFLILATDGLWDVVTNEAAC 359

Query: 285 DAVRMCM---------RAQRSTSSSR-------------------YRGSSSDKSCSDASI 340
             VRMC+         R +  T   R                    RG  +DK+C++AS+
Sbjct: 360 TMVRMCLNRKSGRGRRRGETQTPGRRSEEEGKEEEEKVVGSRKNGKRGEITDKACTEASV 419

BLAST of Lcy09g001580 vs. TAIR 10
Match: AT2G29380.1 (highly ABA-induced PP2C gene 3 )

HSP 1 Score: 355.5 bits (911), Expect = 4.7e-98
Identity = 179/304 (58.88%), Postives = 230/304 (75.66%), Query Frame = 0

Query: 36  RNHDVHKTVKYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSM 95
           +N DV  + +YGV+SVCGRRREMEDAV++HP F++ KN  + P H+FGV+DGHGCSHV+ 
Sbjct: 69  QNVDV-SSPRYGVSSVCGRRREMEDAVAIHPSFSSPKNS-EFPQHYFGVYDGHGCSHVAA 128

Query: 96  SCENRMHEIVKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCEL 155
            C  R+H++V+EE+ S++ + E+WK  M+RSF RMD EV  +   +         C+C+L
Sbjct: 129 RCRERLHKLVQEELSSDMEDEEEWKTTMERSFTRMDKEVVSWGDSV-----VTANCKCDL 188

Query: 156 QTPHQYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIE 215
           QTP   D+VGSTA+V+++ PDK +V+NCGDSRAVLCR    VPLS+DHKPDRPDEL RIE
Sbjct: 189 QTP-ACDSVGSTAVVSVITPDKIVVANCGDSRAVLCRNGKPVPLSTDHKPDRPDELDRIE 248

Query: 216 STGGRVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWD 275
             GGRVIYWD  RVLGVLA SRAIGD YLKPYV  EPEV   DR  +D+ ++LASDGLWD
Sbjct: 249 GAGGRVIYWDCPRVLGVLAMSRAIGDNYLKPYVSCEPEVTITDR-RDDDCLILASDGLWD 308

Query: 276 VVSNESACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVI 335
           VVSNE+AC   RMC+R       +    + SDK+C++AS++LTKLA+A++SSDN+SVVVI
Sbjct: 309 VVSNETACSVARMCLRGGGRRQDNE-DPAISDKACTEASVLLTKLALARNSSDNVSVVVI 362

Query: 336 DLRR 340
           DLRR
Sbjct: 369 DLRR 362

BLAST of Lcy09g001580 vs. TAIR 10
Match: AT5G59220.1 (highly ABA-induced PP2C gene 1 )

HSP 1 Score: 353.6 bits (906), Expect = 1.8e-97
Identity = 194/360 (53.89%), Postives = 236/360 (65.56%), Query Frame = 0

Query: 8   LPRRRRRRRTTDGNAAECIIFPGGVKMQRNHDVHKTV----------KYGVTSVCGRRRE 67
           L RRRRR  T   + +          +     +  TV          KYGV SVCGRRRE
Sbjct: 64  LQRRRRRESTVAASTSTVFYETAKEVVVLCESLSSTVVALPDPEAYPKYGVASVCGRRRE 123

Query: 68  MEDAVSVHPFFATLKNQ-PQNPLHFFGVFDGHGCSHVSMSCENRMHEIVKEEIESELGET 127
           MEDAV+VHPFF+  + +      H+ GV+DGHGCSHV+M C  R+HE+V+EE E++    
Sbjct: 124 MEDAVAVHPFFSRHQTEYSSTGFHYCGVYDGHGCSHVAMKCRERLHELVREEFEAD---- 183

Query: 128 EDWKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCELQTPHQYDTVGSTALVAIVAPD 187
            DW+K M RSF RMD EV   + D   K      CRCELQ P   D VGSTA+V+++ P+
Sbjct: 184 ADWEKSMARSFTRMDMEVVALNADGAAK------CRCELQRP-DCDAVGSTAVVSVLTPE 243

Query: 188 KFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRVIYWDGARVLGVLATS 247
           K IV+NCGDSRAVLCR    + LSSDHKPDRPDEL RI++ GGRVIYWDG RVLGVLA S
Sbjct: 244 KIIVANCGDSRAVLCRNGKAIALSSDHKPDRPDELDRIQAAGGRVIYWDGPRVLGVLAMS 303

Query: 248 RAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNESACDAVRMCMR----A 307
           RAIGD YLKPYVIS PEV   DR   D+F++LASDGLWDVVSNE+AC  VRMC+R     
Sbjct: 304 RAIGDNYLKPYVISRPEVTVTDRANGDDFLILASDGLWDVVSNETACSVVRMCLRGKVNG 363

Query: 308 QRSTSSSRYR------------GSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRRD 341
           Q S+S  R              G   DK+C +AS++LT+LA+A+ SSDN+SVVV+DLRRD
Sbjct: 364 QVSSSPEREMTGVGAGNVVVGGGDLPDKACEEASLLLTRLALARQSSDNVSVVVVDLRRD 412

BLAST of Lcy09g001580 vs. TAIR 10
Match: AT3G11410.1 (protein phosphatase 2CA )

HSP 1 Score: 345.9 bits (886), Expect = 3.7e-95
Identity = 177/298 (59.40%), Postives = 222/298 (74.50%), Query Frame = 0

Query: 45  KYGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEI 104
           K G TSVCGRRR+MEDAVS+HP F  L+   +N  HF+GVFDGHGCSHV+  C  R+H+I
Sbjct: 104 KIGTTSVCGRRRDMEDAVSIHPSF--LQRNSENH-HFYGVFDGHGCSHVAEKCRERLHDI 163

Query: 105 VKEEIESELGETEDWKKLMQRSFARMDDEVEEYSHDI---KQKESSNITCRCELQTPHQY 164
           VK+E+  E+  +++W + M +SF +MD EV +   ++       S   +CRCELQ+P Q 
Sbjct: 164 VKKEV--EVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSP-QC 223

Query: 165 DTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIESTGGRV 224
           D VGSTA+V++V P+K IVSNCGDSRAVLCR    +PLS DHKPDRPDEL RI+  GGRV
Sbjct: 224 DAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRV 283

Query: 225 IYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWDVVSNES 284
           IYWDGARVLGVLA SRAIGD YLKPYVI +PEV   DRT EDE ++LASDGLWDVV NE+
Sbjct: 284 IYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNET 343

Query: 285 ACDAVRMCMRAQRSTSSSRYRGSSSDKSCSDASIVLTKLAMAKHSSDNISVVVIDLRR 340
           AC   RMC+R   +   S     ++  +CSDA+++LTKLA+A+ SSDN+SVVV+DLR+
Sbjct: 344 ACGVARMCLRGAGAGDDS----DAAHNACSDAALLLTKLALARQSSDNVSVVVVDLRK 391

BLAST of Lcy09g001580 vs. TAIR 10
Match: AT5G51760.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 152/311 (48.87%), Postives = 195/311 (62.70%), Query Frame = 0

Query: 46  YGVTSVCGRRREMEDAVSVHPFFATLKNQPQNPLHFFGVFDGHGCSHVSMSCENRMHEIV 105
           YG+ SV GR R+MED+V+V P     +   Q P+HFF V+DGHG S VS  C   MH  V
Sbjct: 109 YGIVSVMGRSRKMEDSVTVKPNLCKPEVNRQRPVHFFAVYDGHGGSQVSTLCSTTMHTFV 168

Query: 106 KEEIESELGETED----------WKKLMQRSFARMDDEVEEYSHDIKQKESSNITCRCEL 165
           KEE+E  L E E+          W+ +M+RSF RMD    E +       +S   C C+ 
Sbjct: 169 KEELEQNLEEEEEGSENDVVERKWRGVMKRSFKRMD----EMATSTCVCGTSVPLCNCD- 228

Query: 166 QTPHQYDTVGSTALVAIVAPDKFIVSNCGDSRAVLCRKSAVVPLSSDHKPDRPDELSRIE 225
             P +    GSTA+ A++  D  IV+N GDSRAVLCR    +PLS+DHKPDRPDE +RIE
Sbjct: 229 --PREAAISGSTAVTAVLTHDHIIVANTGDSRAVLCRNGMAIPLSNDHKPDRPDERARIE 288

Query: 226 STGGRVIYWDGARVLGVLATSRAIGDGYLKPYVISEPEVVEMDRTAEDEFIVLASDGLWD 285
           + GGRV+  DGARV G+LATSRAIGD YLKP V  EPEV  M R + DE +VLASDGLWD
Sbjct: 289 AAGGRVLVVDGARVEGILATSRAIGDRYLKPMVAWEPEVTFMRRESGDECLVLASDGLWD 348

Query: 286 VVSNESACDAVRMCMRAQRSTSSSRYRGSSSDKSCSD--------ASIVLTKLAMAKHSS 339
           V+S++ ACD  R C+R +  +S    R +  D +  +        A+ +LT+LA+ + SS
Sbjct: 349 VLSSQLACDIARFCLREETPSSLDLNRMAQEDDNDGEQNPSRSVLAATLLTRLALGRQSS 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LNW39.2e-9957.41Protein phosphatase 2C 3 OS=Arabidopsis thaliana OX=3702 GN=AIP1 PE=1 SV=1[more]
Q9ZW216.6e-9758.88Probable protein phosphatase 2C 24 OS=Arabidopsis thaliana OX=3702 GN=At2g29380 ... [more]
Q9FIF52.5e-9653.89Probable protein phosphatase 2C 78 OS=Arabidopsis thaliana OX=3702 GN=SAG113 PE=... [more]
P495985.2e-9459.40Protein phosphatase 2C 37 OS=Arabidopsis thaliana OX=3702 GN=PP2CA PE=1 SV=1[more]
Q5N9N26.0e-9055.14Probable protein phosphatase 2C 9 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Match NameE-valueIdentityDescription
A0A6J1JRY93.5e-13372.17Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111489250... [more]
A0A6J1FFR04.6e-13372.59Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114453... [more]
A0A6J1BVK47.8e-12570.20Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A0A0KD797.3e-12371.78Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_6G008720 ... [more]
A0A5A7TJA03.2e-11871.20Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
XP_022993147.17.2e-13372.17probable protein phosphatase 2C 24 isoform X1 [Cucurbita maxima][more]
XP_022939386.19.4e-13372.59probable protein phosphatase 2C 24 isoform X1 [Cucurbita moschata][more]
XP_023551136.11.0e-13170.29probable protein phosphatase 2C 24 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7015982.18.8e-13168.98putative protein phosphatase 2C 24, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022133601.11.6e-12470.20protein phosphatase 2C 3-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G07430.16.6e-10057.41highly ABA-induced PP2C gene 2 [more]
AT2G29380.14.7e-9858.88highly ABA-induced PP2C gene 3 [more]
AT5G59220.11.8e-9753.89highly ABA-induced PP2C gene 1 [more]
AT3G11410.13.7e-9559.40protein phosphatase 2CA [more]
AT5G51760.11.3e-7148.87Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 35..335
e-value: 3.0E-92
score: 322.4
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 45..330
e-value: 7.5E-65
score: 219.1
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 45..337
score: 51.039162
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 44..337
e-value: 1.09073E-88
score: 264.959
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 33..342
e-value: 5.8E-102
score: 343.3
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 40..338
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 37..297
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 303..340
NoneNo IPR availablePANTHERPTHR47992:SF96PROTEIN PHOSPHATASE 2C 3coord: 37..297
coord: 303..340
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 81..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g001580.1Lcy09g001580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity