Lcy09g001540 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g001540
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
LocationChr09: 1307940 .. 1311073 (-)
RNA-Seq ExpressionLcy09g001540
SyntenyLcy09g001540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAATACCCTAACCTTAATTTTAGTGGATAACCAAAAACCACATCCCCAAAACGACACAGTTCCAGTAAACCTCCACATGCCACGTGTCACCCCAGCAACCTTGATTGTGTGGTCGTTAACAATTTGAAAAGCCTATCCACTGCCACTGCCCACATCCAACCTTGAACACTCCAAAGTTCCTTCATTCTCACTGCAAAAATATCATCTTCCCTTCAATTTCCTTCAATTTCAAAGTGTTCAACACTCCAAAGTTCTCTCCTTGGACTCAGTTGTTCTCCTTCCTCTCTCTGAAAACCCATGGCCACCGCCGTCTCCACCGCCGGAGCCGTCAACAGAATTCCGGTAATTGAAATCTTTCCCGCCATTTCAACAACTCACTCAAATGTTGATCACAATTTGCCCTTTCTTCTCTGGTTTTGGTTAGATCTGTTGGGTCTTGTGGATTTCTTAGCGATCATGTGAAAGATTTCAACTTTTGTTAATGCAACAGGAGTAGTAGAGCGAGAAAGGAAGGAGGAGAACAATTGCTCCTTATGTTGAAATGTTAACATCTTAAAGTTTTAGGATTGAAAATTTCTAGCTGTACCCTTGAACGAAATGATTAAGGTTAAAGGGGTTTGTTTATTGCAGGTGAGTTTAAATGGCGCCATCTCTGGAGCTTCAGTTCAGAGCTCTGCATTCTTGGGCAGCAGCTTGAAGAAAGCGAGCTCGAGATTACCCAATTCAAAAATCTCTTCAGGAAGCAGCTTGAAGGTGGTTGCTGTTGTAGAGGATGTTGATGAGAAGAAGCAAACTAATAAGGATAGATGGAAGGGCCTTGCTTCTGATATCTCTGATGATCAACAAGACATCACCAGAGGAAAGGGAATGGTCGACCCAGTCTTCCAAGCTCCTGCTGGACAAGGAACTCATGACCCTGTTCTCAGTTCTTATGAATATCTAAGTGCTGGACTTCGTCAGTAAGTTTTAGAGAACGGCTAGATTTCTTTTTTGTGATTATCAATGATTGGTAATGAAGGAACTGAAAGTCGTGAATGTTGTGGTTTTGATGATGATTTAGGTACAATTTAGACAACAATGTGGATGGCTTTTACATTGCTCCTGCCTTCATGGACAAATTAGTTGTGCACATCTCTAAGAACTTCATGAAACTTCCCAACATCAAGGTTCAACAGAGAACTGAACTGAACTGAACTGATTTATTGTTTGTTAGTTGAGTTTCATTGCTGGGTTTGGCTTTTTCCTTTTACTTATTGATTGATCTGATCATTCTGCCTCAGGTTCCCCTGATTTTGGGGGTTTGGGGAGGCAAAGGTCAAGGAAAATCCTTCCAATGTGAACTTGTCTTTGCCAAGATGGGAATCAAGTGAGTTTCATCTTTCTCCATCCTGTTTGTTCTAGCCATTTCAGATCATTTTCCTGGTTGTGATTCAAACAGTTTGTATTTCTTCAATTGATGCATGCTCTAATGCACTGTTTCATTATTTCAGTCCCATTATGATGAGTGCAGGAGAACTGGAGAGTGGAAATGCAGGAGAACCTGCAAAATTGATTCGACAGAGATACCGTGAAGCAGCGGACATTATCAAGAAGGGAAAAATGTGTGTCCTCTTCATCAACGATCTCGATGCAGGAGCTGGTCGTCTCGGTGGCACCACTCAATACACTGTGAATAACCAGATGGTTAATGCTACCCTCATGAACATAGCTGATAACCCAACCAATGTCCAGCTCCCTGGTATGTACAACAAAGAGGAAAATCCTCGTGTTCCCATCGTTGTCACGGGTAACGACTTCTCAACGTTGTATGCTCCTCTCATCCGTGACGGTCGTATGGAAAAATTCTATTGGGCACCTACTCGAGAAGATAGAATTGGTGTCTGCACAGGAATCTTTAGGAGTGACAATGTTGCTAAAGAAGACATTATCAAACTTGTTGATACATTCCCTGGCCAATCTATCGGTGAGCTTCCAAGTCTAAACTATGAATCATAAATACATCAATATTACGATTCGTTTCCCTTCTATATTCCAACCTAATAGATTAAACCATGATCATACAGATTTCTTTGGTGCCCTGAGGGCAAGAGTATACGATGACGAAGTGAGGAGGTGGGCTGCTTCAGTCGGAGTTGAGAATATCGCTAAGAAGCTCGTCAACTCAAAGGAAGGACCACCGAAGTTCGAGCAGCCAAAGATGAACTTGGAGAAGCTCCTCGAGTATGGATACATGCTCGTTCAGGAGCAAGAGAACGTGAAGAGAGTCCAATTGGCTGACAAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAATGAAGACGCTATAAAAAGTGGAGCATTCTACGGTTAGTTCTCTCTTTGATCTTTTATAGATCGTAAAGAATTGTATAAAACATAAGCAGCTTCTCCTGAGCTGTTGCTTCTGTTTGTTGCAGGCAAGGCCGCACAGCAGGTGAGTTTTCCTGTCCCTGAAGGTTGCACAGACCCAAATGCAGAAAACTTCGACCCAACTGCTAGGAGTGATGATGGAAGCTGCAATTATGAATTATAAGCCTTCCCTTTAGCTGTTTGGTGGAAGATCATTTACCACTTATGTTTGATAAGGAGAACACTGTCACCTGTTTGTATGAAAATATCCACTGGTCTGCTCTACTCTATGCTTCAAACTTGGTGGGGGGTTTCAGTTCTCATTCTCATTTCTTTTTCTTTTTTCCCTTTGTTGCCACGACATTGACATCTTTTGTTGGTGTTGATAAAGAACATAATCTGGTGTCTCAAAGTTTTTTGTGATATTGAGATCTTTTCTCAGTCTTGGTTATACCATACATCCAGAAAATGAATGTGAAATCATTCAGGGTCGAAAACCTTTAAGGCAAACAGATTCCTAAGAACAGCATCGTATAATATCTATAGAGATGATGAAACTGTTCTATCAGAACAACACAAACACAGCAGTGCCTCTTAGATTTATTTTATCAATAGAATAGACCTGGGATATGACAGAAAGCAAGAAAAAACCACAATTCTAACGAGGATCTTGCAACTAAAAGCAATGGGGAAGAATTGCACAATGAGTGTTTTATCTAACAGTTCCATTGATAAAGTTCATGCGATGGCAATCTCAAAGAAATG

mRNA sequence

GTGAATACCCTAACCTTAATTTTAGTGGATAACCAAAAACCACATCCCCAAAACGACACAGTTCCAGTAAACCTCCACATGCCACGTGTCACCCCAGCAACCTTGATTGTGTGGTCGTTAACAATTTGAAAAGCCTATCCACTGCCACTGCCCACATCCAACCTTGAACACTCCAAAGTTCCTTCATTCTCACTGCAAAAATATCATCTTCCCTTCAATTTCCTTCAATTTCAAAGTGTTCAACACTCCAAAGTTCTCTCCTTGGACTCAGTTGTTCTCCTTCCTCTCTCTGAAAACCCATGGCCACCGCCGTCTCCACCGCCGGAGCCGTCAACAGAATTCCGGTGAGTTTAAATGGCGCCATCTCTGGAGCTTCAGTTCAGAGCTCTGCATTCTTGGGCAGCAGCTTGAAGAAAGCGAGCTCGAGATTACCCAATTCAAAAATCTCTTCAGGAAGCAGCTTGAAGGTGGTTGCTGTTGTAGAGGATGTTGATGAGAAGAAGCAAACTAATAAGGATAGATGGAAGGGCCTTGCTTCTGATATCTCTGATGATCAACAAGACATCACCAGAGGAAAGGGAATGGTCGACCCAGTCTTCCAAGCTCCTGCTGGACAAGGAACTCATGACCCTGTTCTCAGTTCTTATGAATATCTAAGTGCTGGACTTCGTCAGTACAATTTAGACAACAATGTGGATGGCTTTTACATTGCTCCTGCCTTCATGGACAAATTAGTTGTGCACATCTCTAAGAACTTCATGAAACTTCCCAACATCAAGGTTCCCCTGATTTTGGGGGTTTGGGGAGGCAAAGGTCAAGGAAAATCCTTCCAATGTGAACTTGTCTTTGCCAAGATGGGAATCAATCCCATTATGATGAGTGCAGGAGAACTGGAGAGTGGAAATGCAGGAGAACCTGCAAAATTGATTCGACAGAGATACCGTGAAGCAGCGGACATTATCAAGAAGGGAAAAATGTGTGTCCTCTTCATCAACGATCTCGATGCAGGAGCTGGTCGTCTCGGTGGCACCACTCAATACACTGTGAATAACCAGATGGTTAATGCTACCCTCATGAACATAGCTGATAACCCAACCAATGTCCAGCTCCCTGGTATGTACAACAAAGAGGAAAATCCTCGTGTTCCCATCGTTGTCACGGGTAACGACTTCTCAACGTTGTATGCTCCTCTCATCCGTGACGGTCGTATGGAAAAATTCTATTGGGCACCTACTCGAGAAGATAGAATTGGTGTCTGCACAGGAATCTTTAGGAGTGACAATGTTGCTAAAGAAGACATTATCAAACTTGTTGATACATTCCCTGGCCAATCTATCGATTTCTTTGGTGCCCTGAGGGCAAGAGTATACGATGACGAAGTGAGGAGGTGGGCTGCTTCAGTCGGAGTTGAGAATATCGCTAAGAAGCTCGTCAACTCAAAGGAAGGACCACCGAAGTTCGAGCAGCCAAAGATGAACTTGGAGAAGCTCCTCGAGTATGGATACATGCTCGTTCAGGAGCAAGAGAACGTGAAGAGAGTCCAATTGGCTGACAAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAATGAAGACGCTATAAAAAGTGGAGCATTCTACGGCAAGGCCGCACAGCAGGTGAGTTTTCCTGTCCCTGAAGGTTGCACAGACCCAAATGCAGAAAACTTCGACCCAACTGCTAGGAGTGATGATGGAAGCTGCAATTATGAATTATAAGCCTTCCCTTTAGCTGTTTGGTGGAAGATCATTTACCACTTATGTTTGATAAGGAGAACACTGTCACCTGTTTGTATGAAAATATCCACTGGTCTGCTCTACTCTATGCTTCAAACTTGGTGGGGGGTTTCAGTTCTCATTCTCATTTCTTTTTCTTTTTTCCCTTTGTTGCCACGACATTGACATCTTTTGTTGGTGTTGATAAAGAACATAATCTGGTGTCTCAAAGTTTTTTGTGATATTGAGATCTTTTCTCAGTCTTGGTTATACCATACATCCAGAAAATGAATGTGAAATCATTCAGGGTCGAAAACCTTTAAGGCAAACAGATTCCTAAGAACAGCATCGTATAATATCTATAGAGATGATGAAACTGTTCTATCAGAACAACACAAACACAGCAGTGCCTCTTAGATTTATTTTATCAATAGAATAGACCTGGGATATGACAGAAAGCAAGAAAAAACCACAATTCTAACGAGGATCTTGCAACTAAAAGCAATGGGGAAGAATTGCACAATGAGTGTTTTATCTAACAGTTCCATTGATAAAGTTCATGCGATGGCAATCTCAAAGAAATG

Coding sequence (CDS)

ATGGCCACCGCCGTCTCCACCGCCGGAGCCGTCAACAGAATTCCGGTGAGTTTAAATGGCGCCATCTCTGGAGCTTCAGTTCAGAGCTCTGCATTCTTGGGCAGCAGCTTGAAGAAAGCGAGCTCGAGATTACCCAATTCAAAAATCTCTTCAGGAAGCAGCTTGAAGGTGGTTGCTGTTGTAGAGGATGTTGATGAGAAGAAGCAAACTAATAAGGATAGATGGAAGGGCCTTGCTTCTGATATCTCTGATGATCAACAAGACATCACCAGAGGAAAGGGAATGGTCGACCCAGTCTTCCAAGCTCCTGCTGGACAAGGAACTCATGACCCTGTTCTCAGTTCTTATGAATATCTAAGTGCTGGACTTCGTCAGTACAATTTAGACAACAATGTGGATGGCTTTTACATTGCTCCTGCCTTCATGGACAAATTAGTTGTGCACATCTCTAAGAACTTCATGAAACTTCCCAACATCAAGGTTCCCCTGATTTTGGGGGTTTGGGGAGGCAAAGGTCAAGGAAAATCCTTCCAATGTGAACTTGTCTTTGCCAAGATGGGAATCAATCCCATTATGATGAGTGCAGGAGAACTGGAGAGTGGAAATGCAGGAGAACCTGCAAAATTGATTCGACAGAGATACCGTGAAGCAGCGGACATTATCAAGAAGGGAAAAATGTGTGTCCTCTTCATCAACGATCTCGATGCAGGAGCTGGTCGTCTCGGTGGCACCACTCAATACACTGTGAATAACCAGATGGTTAATGCTACCCTCATGAACATAGCTGATAACCCAACCAATGTCCAGCTCCCTGGTATGTACAACAAAGAGGAAAATCCTCGTGTTCCCATCGTTGTCACGGGTAACGACTTCTCAACGTTGTATGCTCCTCTCATCCGTGACGGTCGTATGGAAAAATTCTATTGGGCACCTACTCGAGAAGATAGAATTGGTGTCTGCACAGGAATCTTTAGGAGTGACAATGTTGCTAAAGAAGACATTATCAAACTTGTTGATACATTCCCTGGCCAATCTATCGATTTCTTTGGTGCCCTGAGGGCAAGAGTATACGATGACGAAGTGAGGAGGTGGGCTGCTTCAGTCGGAGTTGAGAATATCGCTAAGAAGCTCGTCAACTCAAAGGAAGGACCACCGAAGTTCGAGCAGCCAAAGATGAACTTGGAGAAGCTCCTCGAGTATGGATACATGCTCGTTCAGGAGCAAGAGAACGTGAAGAGAGTCCAATTGGCTGACAAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAATGAAGACGCTATAAAAAGTGGAGCATTCTACGGCAAGGCCGCACAGCAGGTGAGTTTTCCTGTCCCTGAAGGTTGCACAGACCCAAATGCAGAAAACTTCGACCCAACTGCTAGGAGTGATGATGGAAGCTGCAATTATGAATTATAA

Protein sequence

MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAVVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLSAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDEVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKYLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL
Homology
BLAST of Lcy09g001540 vs. ExPASy Swiss-Prot
Match: Q7X9A0 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA1 PE=1 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 3.2e-222
Identity = 391/480 (81.46%), Postives = 431/480 (89.79%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGAS-VQSSAFLGSSLKKASSRLPNSKISSGSSLKVVA 60
           MA A ST GAVNR P+SLNG+ + AS V S+AF GSSLKK++++ P  K SSG + K+VA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKKSAAKFP--KASSG-NFKIVA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
             +++ E +QT+KD+WKGLA DISDDQQDITRGKGMVD +FQAP   GTH  V+SSY+Y+
Sbjct: 61  --QEISEDQQTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYDYI 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLRQYNLDNN+DGFYIAPAFMDKLVVHI+KNF+ LPNIK+PLILG+WGGKGQGKSFQC
Sbjct: 121 SQGLRQYNLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMC LFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC GIFR+DNVA +DI+KLVDTFPGQSIDFFGALRARVY D
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARVYHD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR+W + VGV+ I KKLVNSKEGPP FEQPKM ++KLL YG MLVQEQENVKRVQLADK
Sbjct: 361 EVRKWVSEVGVDTIGKKLVNSKEGPPSFEQPKMTIDKLLGYGGMLVQEQENVKRVQLADK 420

Query: 421 YLSEAALGDANEDAIKSGAFY-GKAAQQV-SFPVPEGCTDPNAENFDPTARSDDGSCNYE 478
           Y+SEAALGDAN DAIK G FY G+AAQQV + PVPEGCTDP A N+DPTARSDDGSC Y+
Sbjct: 421 YMSEAALGDANNDAIKRGTFYGGQAAQQVGNVPVPEGCTDPQATNYDPTARSDDGSCVYK 475

BLAST of Lcy09g001540 vs. ExPASy Swiss-Prot
Match: P10896 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RCA PE=1 SV=2)

HSP 1 Score: 749.2 bits (1933), Expect = 2.9e-215
Identity = 380/477 (79.66%), Postives = 410/477 (85.95%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGA-SVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVA 60
           MA AVST GA+NR P+SLNG+ SGA S  +S FLG  +    SR   S   S  S KV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED    KQT+ DRW+GLA D SDDQQDITRGKGMVD VFQAP G GTH  VLSSYEY+
Sbjct: 61  VKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYV 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLRQYNLDN +DGFYIAPAFMDKLVVHI+KNF+ LPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGAG
Sbjct: 181 ELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPI+ TGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC GIFR+D +  EDI+ LVD FPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR++  S+GVE I K+LVNS+EGPP FEQP+M  EKL+EYG MLV EQENVKRVQLA+ 
Sbjct: 361 EVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAET 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNY 477
           YLS+AALGDAN DAI  G FYGK AQQV+ PVPEGCTDP AENFDPTARSDDG+C Y
Sbjct: 421 YLSQAALGDANADAIGRGTFYGKGAQQVNLPVPEGCTDPVAENFDPTARSDDGTCVY 472

BLAST of Lcy09g001540 vs. ExPASy Swiss-Prot
Match: Q40281 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica OX=3750 GN=RCA PE=2 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 1.9e-214
Identity = 372/441 (84.35%), Postives = 399/441 (90.48%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAV 60
           MATAVST G+VNR P +LNG+ S ASV SS FLGSSLKK +SR  NSK+SSGS    + +
Sbjct: 1   MATAVSTIGSVNRAPPNLNGSSSSASVPSSTFLGSSLKKVNSRFTNSKVSSGS----LRI 60

Query: 61  VEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLS 120
           V  VDE KQT+KDRWKGLA D SDDQQDITRGKG VD +FQAP G GTH  ++SSYEY+S
Sbjct: 61  VASVDEDKQTDKDRWKGLAFDTSDDQQDITRGKGKVDSLFQAPQGSGTHFAIMSSYEYIS 120

Query: 121 AGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180
            GLRQYN DNN+DG+YIAPAFMDKLVVHI+KNFM LPN+KVPLILG+WGGKGQGKSFQCE
Sbjct: 121 TGLRQYNFDNNMDGYYIAPAFMDKLVVHITKNFMTLPNMKVPLILGIWGGKGQGKSFQCE 180

Query: 181 LVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240
           LVFAKM I+PIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKMC LFINDLDAGAGR
Sbjct: 181 LVFAKMRISPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCALFINDLDAGAGR 240

Query: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIR 300
           LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLIR
Sbjct: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIR 300

Query: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDE 360
           DGRMEKFYWAPTREDRIGVC GIFRSDNVAKEDI+KLVDTFPGQSIDFFGALRARVYDDE
Sbjct: 301 DGRMEKFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVYDDE 360

Query: 361 VRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKY 420
           VR+W   VGV++I KKLVNSKEGPP FEQPKM +EKLLEYG MLVQEQENVKRVQLADKY
Sbjct: 361 VRKWITGVGVDSIGKKLVNSKEGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLADKY 420

Query: 421 LSEAALGDANEDAIKSGAFYG 442
           LSEAALGDAN DA+ +G FYG
Sbjct: 421 LSEAALGDANSDAMNTGTFYG 437

BLAST of Lcy09g001540 vs. ExPASy Swiss-Prot
Match: P93431 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RCA PE=1 SV=2)

HSP 1 Score: 741.5 bits (1913), Expect = 6.0e-213
Identity = 360/446 (80.72%), Postives = 399/446 (89.46%), Query Frame = 0

Query: 32  FLGSSLKKASSRLPNSKISSGSSLKVVAVVEDVDEKKQTNKDRWKGLASDISDDQQDITR 91
           FLG  LKK  +   N    S +  +   + +++DE KQT++DRWKGLA DISDDQQDITR
Sbjct: 19  FLGKKLKKQVTSAVNYHGKSSNINRFKVMAKELDEGKQTDQDRWKGLAYDISDDQQDITR 78

Query: 92  GKGMVDPVFQAPAGQGTHDPVLSSYEYLSAGLRQYNLDNNVDGFYIAPAFMDKLVVHISK 151
           GKG VD +FQAP G GTH+ VLSSYEYLS GLR Y+ DN + GFYIAPAFMDKLVVHISK
Sbjct: 79  GKGFVDSLFQAPTGDGTHEAVLSSYEYLSQGLRTYDFDNTMGGFYIAPAFMDKLVVHISK 138

Query: 152 NFMKLPNIKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIR 211
           NFM LPNIKVPLILG+WGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIR
Sbjct: 139 NFMTLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIR 198

Query: 212 QRYREAADIIKKGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLP 271
           QRYREAADIIKKGKMC LFINDLDAGAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLP
Sbjct: 199 QRYREAADIIKKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLP 258

Query: 272 GMYNKEENPRVPIVVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCTGIFRSDNVAK 331
           GMYNKE+NPRVPI+VTGNDFSTLYAPLIRDGRMEKFYWAPTR+DR+GVC GIFR+DNV  
Sbjct: 259 GMYNKEDNPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCKGIFRTDNVPD 318

Query: 332 EDIIKLVDTFPGQSIDFFGALRARVYDDEVRRWAASVGVENIAKKLVNSKEGPPKFEQPK 391
           EDI+K+VD+FPGQSIDFFGALRARVYDDEVR+W +  GVENI K+LVNS+EGPP+FEQPK
Sbjct: 319 EDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDTGVENIGKRLVNSREGPPEFEQPK 378

Query: 392 MNLEKLLEYGYMLVQEQENVKRVQLADKYLSEAALGDANEDAIKSGAFYGKAAQQV-SFP 451
           M +EKL+EYGYMLV+EQENVKRVQLA++YLSEAALGDAN DA+K+G+FYG+ AQQ  + P
Sbjct: 379 MTIEKLMEYGYMLVKEQENVKRVQLAEQYLSEAALGDANSDAMKTGSFYGQGAQQAGNLP 438

Query: 452 VPEGCTDPNAENFDPTARSDDGSCNY 477
           VPEGCTDP A+NFDPTARSDDGSC Y
Sbjct: 439 VPEGCTDPVAKNFDPTARSDDGSCLY 464

BLAST of Lcy09g001540 vs. ExPASy Swiss-Prot
Match: P10871 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2)

HSP 1 Score: 731.1 bits (1886), Expect = 8.1e-210
Identity = 361/479 (75.37%), Postives = 411/479 (85.80%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASS-RLPNSKISSGSSLKVVA 60
           MATAVST GA  R P++LNG+ +GASV +S FLGSSLKK ++ R P+S  ++  ++K   
Sbjct: 1   MATAVSTVGAATRAPLNLNGSSAGASVPTSGFLGSSLKKHTNVRFPSSSRTTSMTVKAA- 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
                 E ++ N D+W  LA D SDDQ DI RGKGMVD +FQAPA  GTH P+ SS+EY 
Sbjct: 61  ------ENEEKNTDKWAHLAKDFSDDQLDIRRGKGMVDSLFQAPADAGTHVPIQSSFEYE 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLR+Y++DN +  FYIAPAFMDKLVVHI+KNF+ LPNIK+PLILGVWGGKGQGKSFQC
Sbjct: 121 SQGLRKYDIDNMLGDFYIAPAFMDKLVVHITKNFLNLPNIKIPLILGVWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAK+GINPIMMSAGELESGNAGEPAKLIRQRYREAAD+I KGKMC LFINDL+ GAG
Sbjct: 181 ELVFAKLGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIAKGKMCALFINDLEPGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNK++N RVPI+VTGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQDNARVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVCTGIF++D V  E ++KLVD FPGQSIDFFGALRARVY D
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCTGIFKTDKVPAEHVVKLVDAFPGQSIDFFGALRARVYHD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR+W  SVGV+N+ KKLVNSK+GPP FEQP+M L+KL+EYG MLVQEQENVKRVQLAD+
Sbjct: 361 EVRKWVNSVGVDNVGKKLVNSKDGPPVFEQPEMTLQKLMEYGNMLVQEQENVKRVQLADQ 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           Y+S AALGDAN+DAI  G F+GKAAQQVS PV +GCTDP A+N+DPTARSDDGSC Y L
Sbjct: 421 YMSSAALGDANKDAIDRGTFFGKAAQQVSLPVAQGCTDPEAKNYDPTARSDDGSCTYNL 472

BLAST of Lcy09g001540 vs. ExPASy TrEMBL
Match: A0A6J1JKS4 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487787 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.1e-257
Identity = 447/478 (93.51%), Postives = 465/478 (97.28%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAV 60
           MATA+STAGAVNRIPVSLNGAISGASVQSSAFLGSSLKK +SR PNSK+S GSSLKVVAV
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSRYPNSKVSWGSSLKVVAV 60

Query: 61  VEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLS 120
            E++DEKKQT+KDRWKGLA DISDDQQDITRGKG+VD +FQAP G GTHDPVL+SYEYLS
Sbjct: 61  AEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLNSYEYLS 120

Query: 121 AGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180
           AGLRQYNLDNNVDGFYIAP+FMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE
Sbjct: 121 AGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180

Query: 181 LVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240
           LV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR
Sbjct: 181 LVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240

Query: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIR 300
           LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPLIR
Sbjct: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLIR 300

Query: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDE 360
           DGRMEKFYWAPTREDRIGVCTGIFRSDNV KEDI+KLVDTFPGQSIDFFGALRARVYDDE
Sbjct: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDDE 360

Query: 361 VRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKY 420
           VR+WAASVGVENI+K+LVNSKEGPP FEQPKM LEKLLEYG MLVQEQENVKRVQLADKY
Sbjct: 361 VRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADKY 420

Query: 421 LSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           LSEAALGDANEDAIKSGAFYGKAAQQV+FPVPEGCTDPNAENFDPTARSD+GSCNYEL
Sbjct: 421 LSEAALGDANEDAIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of Lcy09g001540 vs. ExPASy TrEMBL
Match: A0A6J1GTI7 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111457396 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.1e-257
Identity = 447/478 (93.51%), Postives = 465/478 (97.28%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAV 60
           MATA+STAGAVNRIPVSLNGAISGASVQSSAFLGSSLKK +SR PNSK+S GSSLKVVAV
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSRYPNSKVSWGSSLKVVAV 60

Query: 61  VEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLS 120
            E++DEKKQT+KDRWKGLA DISDDQQDITRGKG+VD +FQAP G GTHDPVLSSYEYLS
Sbjct: 61  AEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLSSYEYLS 120

Query: 121 AGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180
           AGLRQYNLDNNVDGFYIAP+FMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE
Sbjct: 121 AGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180

Query: 181 LVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240
           LV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR
Sbjct: 181 LVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240

Query: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIR 300
           LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPLIR
Sbjct: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLIR 300

Query: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDE 360
           DGRMEKFYWAPTREDRIGVCTGIFRSDNV KEDI+KLVDTFPGQSIDFFGALRARVYDDE
Sbjct: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDDE 360

Query: 361 VRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKY 420
           VR+WAASVGVENI+K+LVNSKEGPP FEQPKM LEKLLEYG MLVQEQENVKRVQLADKY
Sbjct: 361 VRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADKY 420

Query: 421 LSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           LSEAALGDANED+IKSGAFYGKAAQQV+FPVPEGCTDPNAENFDPTARSD+GSCNYEL
Sbjct: 421 LSEAALGDANEDSIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of Lcy09g001540 vs. ExPASy TrEMBL
Match: A0A6J1FKE0 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111444819 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.0e-252
Identity = 441/479 (92.07%), Postives = 459/479 (95.82%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSL-KVVA 60
           MA AVSTAGA N + +SLNGAI+GASVQ+SAFLGSSLKKA+ R PNSK+SSG SL KVVA
Sbjct: 1   MAAAVSTAGATNGVSLSLNGAIAGASVQNSAFLGSSLKKANFRYPNSKVSSGRSLMKVVA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED+DEKKQT+KDRWKGLA DISDDQQDITRGKGMVD +FQAP G GTHDPVLSSYEYL
Sbjct: 61  VAEDIDEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN VDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC+GIFR+DNV KEDIIKLVDTFPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR+WAASVGVENI+K+LVNSKEGPP FEQPKM L+KLLEYG MLVQEQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           YLSEAALGDANEDAIKSGAFYGKAAQQV  PVPEGCTDPNAEN+DPTARSDDGSCNYEL
Sbjct: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 479

BLAST of Lcy09g001540 vs. ExPASy TrEMBL
Match: A0A0A0KBG2 (ATPase_AAA_core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008680 PE=4 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 2.0e-251
Identity = 433/474 (91.35%), Postives = 454/474 (95.78%), Query Frame = 0

Query: 5   VSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAVVEDV 64
           ++TAGAVNRIP+SLNGAI+G SVQSSAFLGSSLKKA+ R P+SK+SSGSS KVVAV E++
Sbjct: 1   MATAGAVNRIPLSLNGAIAGPSVQSSAFLGSSLKKANFRFPSSKVSSGSSFKVVAVAEEI 60

Query: 65  DEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLSAGLR 124
           DEKKQT+KD+WKGLA D+SDDQQDITRGKGMVD +FQAP+G GTHDPVLSSYEYLSAGLR
Sbjct: 61  DEKKQTSKDKWKGLAFDVSDDQQDITRGKGMVDTLFQAPSGAGTHDPVLSSYEYLSAGLR 120

Query: 125 QYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCELVFA 184
           QYN DNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILG+WGGKGQGKSFQCELVFA
Sbjct: 121 QYNFDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQCELVFA 180

Query: 185 KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT 244
           KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMC LFINDLDAGAGRLGGT
Sbjct: 181 KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCALFINDLDAGAGRLGGT 240

Query: 245 TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIRDGRM 304
           TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLIRDGRM
Sbjct: 241 TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM 300

Query: 305 EKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDEVRRW 364
           EKFYWAPTREDRIGVC+GIFRSDN+ KEDI+KLVDTFPGQSIDFFGALRARVYDDEVR W
Sbjct: 301 EKFYWAPTREDRIGVCSGIFRSDNIPKEDIVKLVDTFPGQSIDFFGALRARVYDDEVRNW 360

Query: 365 AASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKYLSEA 424
             SVGVENIAKKLVNSKEGPP FEQPKM LEKLLEYG MLVQEQENVKRVQLADKYLSEA
Sbjct: 361 VCSVGVENIAKKLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADKYLSEA 420

Query: 425 ALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           ALGDANEDAIKSG FYGKAAQQVS P+PEGCTDPNA+NFDPTARSDDGSCNY L
Sbjct: 421 ALGDANEDAIKSGTFYGKAAQQVSLPIPEGCTDPNADNFDPTARSDDGSCNYVL 474

BLAST of Lcy09g001540 vs. ExPASy TrEMBL
Match: A0A6J1JQC9 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488905 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 436/474 (91.98%), Postives = 456/474 (96.20%), Query Frame = 0

Query: 6   STAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSL-KVVAVVEDV 65
           +TAGAVN + +SLNGAI+GASVQ+SAFLGSSLKKA+ R PNS++SSG SL KVVAV ED+
Sbjct: 3   ATAGAVNGVSLSLNGAIAGASVQNSAFLGSSLKKANFRYPNSRVSSGRSLMKVVAVAEDI 62

Query: 66  DEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLSAGLR 125
           DEKKQT+KDRWKGLA DISDDQQDITRGKGMVD +FQAP G GTHDPVLSSYEYLSAGLR
Sbjct: 63  DEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYLSAGLR 122

Query: 126 QYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCELVFA 185
           QYNLDN VDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILG+WGGKGQGKSFQCELVFA
Sbjct: 123 QYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQCELVFA 182

Query: 186 KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT 245
           KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT
Sbjct: 183 KMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGRLGGT 242

Query: 246 TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIRDGRM 305
           TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLIRDGRM
Sbjct: 243 TQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM 302

Query: 306 EKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDEVRRW 365
           EKFYWAPTREDRIGVC+GIFR+DNV KEDIIKLVDTFPGQSIDFFGALRARVYDDEVR+W
Sbjct: 303 EKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYDDEVRKW 362

Query: 366 AASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKYLSEA 425
           AASVGVENI+K+LVNSKEGPP FEQPKM L+KLLEYG MLVQEQENVKRVQLADKYLSEA
Sbjct: 363 AASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLADKYLSEA 422

Query: 426 ALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           ALGDANEDAIKSGAFYGKAAQQV  PVPEGCTDPNAEN+DPTARSDDGSCNYEL
Sbjct: 423 ALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 476

BLAST of Lcy09g001540 vs. NCBI nr
Match: XP_022955346.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita moschata] >XP_023552446.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6602653.1 Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033339.1 Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 896.7 bits (2316), Expect = 8.5e-257
Identity = 447/478 (93.51%), Postives = 465/478 (97.28%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAV 60
           MATA+STAGAVNRIPVSLNGAISGASVQSSAFLGSSLKK +SR PNSK+S GSSLKVVAV
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSRYPNSKVSWGSSLKVVAV 60

Query: 61  VEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLS 120
            E++DEKKQT+KDRWKGLA DISDDQQDITRGKG+VD +FQAP G GTHDPVLSSYEYLS
Sbjct: 61  AEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLSSYEYLS 120

Query: 121 AGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180
           AGLRQYNLDNNVDGFYIAP+FMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE
Sbjct: 121 AGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180

Query: 181 LVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240
           LV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR
Sbjct: 181 LVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240

Query: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIR 300
           LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPLIR
Sbjct: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLIR 300

Query: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDE 360
           DGRMEKFYWAPTREDRIGVCTGIFRSDNV KEDI+KLVDTFPGQSIDFFGALRARVYDDE
Sbjct: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDDE 360

Query: 361 VRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKY 420
           VR+WAASVGVENI+K+LVNSKEGPP FEQPKM LEKLLEYG MLVQEQENVKRVQLADKY
Sbjct: 361 VRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADKY 420

Query: 421 LSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           LSEAALGDANED+IKSGAFYGKAAQQV+FPVPEGCTDPNAENFDPTARSD+GSCNYEL
Sbjct: 421 LSEAALGDANEDSIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of Lcy09g001540 vs. NCBI nr
Match: XP_022991087.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 896.7 bits (2316), Expect = 8.5e-257
Identity = 447/478 (93.51%), Postives = 465/478 (97.28%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVAV 60
           MATA+STAGAVNRIPVSLNGAISGASVQSSAFLGSSLKK +SR PNSK+S GSSLKVVAV
Sbjct: 1   MATALSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKVNSRYPNSKVSWGSSLKVVAV 60

Query: 61  VEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYLS 120
            E++DEKKQT+KDRWKGLA DISDDQQDITRGKG+VD +FQAP G GTHDPVL+SYEYLS
Sbjct: 61  AEEIDEKKQTDKDRWKGLAYDISDDQQDITRGKGLVDSLFQAPTGAGTHDPVLNSYEYLS 120

Query: 121 AGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180
           AGLRQYNLDNNVDGFYIAP+FMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE
Sbjct: 121 AGLRQYNLDNNVDGFYIAPSFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQCE 180

Query: 181 LVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240
           LV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR
Sbjct: 181 LVLAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAGR 240

Query: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLIR 300
           LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPI+VTGNDFSTLYAPLIR
Sbjct: 241 LGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLIR 300

Query: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDDE 360
           DGRMEKFYWAPTREDRIGVCTGIFRSDNV KEDI+KLVDTFPGQSIDFFGALRARVYDDE
Sbjct: 301 DGRMEKFYWAPTREDRIGVCTGIFRSDNVPKEDIVKLVDTFPGQSIDFFGALRARVYDDE 360

Query: 361 VRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADKY 420
           VR+WAASVGVENI+K+LVNSKEGPP FEQPKM LEKLLEYG MLVQEQENVKRVQLADKY
Sbjct: 361 VRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADKY 420

Query: 421 LSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           LSEAALGDANEDAIKSGAFYGKAAQQV+FPVPEGCTDPNAENFDPTARSD+GSCNYEL
Sbjct: 421 LSEAALGDANEDAIKSGAFYGKAAQQVNFPVPEGCTDPNAENFDPTARSDNGSCNYEL 478

BLAST of Lcy09g001540 vs. NCBI nr
Match: XP_038886619.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Benincasa hispida])

HSP 1 Score: 889.0 bits (2296), Expect = 1.8e-254
Identity = 446/479 (93.11%), Postives = 461/479 (96.24%), Query Frame = 0

Query: 1   MAT-AVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVA 60
           MAT AVSTAGAVNRIP+SLNGAI+G SVQSSAFLG+SLKKA+ R PNSK+SSGSSLKVVA
Sbjct: 1   MATAAVSTAGAVNRIPLSLNGAIAGPSVQSSAFLGNSLKKANLRYPNSKVSSGSSLKVVA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V E++DEKKQTNKD+WKGLA DISDDQQDITRGKGMVD +FQAP+G GTHDPVLSSYEYL
Sbjct: 61  VAEEIDEKKQTNKDKWKGLAFDISDDQQDITRGKGMVDTLFQAPSGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC+GIFRSDNV KED++KLVDTFPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCSGIFRSDNVPKEDVVKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR WA SVGVENIAKKLVNSKEGPP FEQPKM LEKLLEYG MLVQEQENVKRVQLADK
Sbjct: 361 EVRNWAISVGVENIAKKLVNSKEGPPTFEQPKMTLEKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           YLSEAALGDANEDAIKSG FYGKAAQQV  PVPEGCTDPNA+NFDPTARSDDGSCNY L
Sbjct: 421 YLSEAALGDANEDAIKSGTFYGKAAQQVHLPVPEGCTDPNADNFDPTARSDDGSCNYVL 479

BLAST of Lcy09g001540 vs. NCBI nr
Match: XP_023550769.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.7 bits (2277), Expect = 2.8e-252
Identity = 442/479 (92.28%), Postives = 460/479 (96.03%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSL-KVVA 60
           MA AVSTAGAVN + +SLNGAI+GASVQ+SAFLGSSLKKA+ R PNSK+SSG SL KVVA
Sbjct: 1   MAAAVSTAGAVNGVSLSLNGAIAGASVQNSAFLGSSLKKANFRYPNSKVSSGRSLMKVVA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED+DEKKQT+KDRWKGLA DISDDQQDITRGKGMVD +FQAP G GTHDPVLSSYEYL
Sbjct: 61  VAEDIDEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN VDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC+GIFR+DNV KEDIIKLVDTFPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR+WAASVGVENI+K+LVNSKEGPP FEQPKM L+KLLEYG MLVQEQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           YLSEAALGDANEDAIKSGAFYGKAAQQV  PVPEGCTDPNAEN+DPTARSDDGSCNYEL
Sbjct: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 479

BLAST of Lcy09g001540 vs. NCBI nr
Match: XP_022938660.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita moschata] >KAG6578415.1 Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880.2 bits (2273), Expect = 8.2e-252
Identity = 441/479 (92.07%), Postives = 459/479 (95.82%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGASVQSSAFLGSSLKKASSRLPNSKISSGSSL-KVVA 60
           MA AVSTAGA N + +SLNGAI+GASVQ+SAFLGSSLKKA+ R PNSK+SSG SL KVVA
Sbjct: 1   MAAAVSTAGATNGVSLSLNGAIAGASVQNSAFLGSSLKKANFRYPNSKVSSGRSLMKVVA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED+DEKKQT+KDRWKGLA DISDDQQDITRGKGMVD +FQAP G GTHDPVLSSYEYL
Sbjct: 61  VAEDIDEKKQTDKDRWKGLAYDISDDQQDITRGKGMVDSLFQAPTGAGTHDPVLSSYEYL 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           SAGLRQYNLDN VDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SAGLRQYNLDNTVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG
Sbjct: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPI+VTGNDFSTLYAPLI
Sbjct: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC+GIFR+DNV KEDIIKLVDTFPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCSGIFRTDNVPKEDIIKLVDTFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR+WAASVGVENI+K+LVNSKEGPP FEQPKM L+KLLEYG MLVQEQENVKRVQLADK
Sbjct: 361 EVRKWAASVGVENISKRLVNSKEGPPTFEQPKMTLQKLLEYGNMLVQEQENVKRVQLADK 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNYEL 479
           YLSEAALGDANEDAIKSGAFYGKAAQQV  PVPEGCTDPNAEN+DPTARSDDGSCNYEL
Sbjct: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVHLPVPEGCTDPNAENYDPTARSDDGSCNYEL 479

BLAST of Lcy09g001540 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 749.2 bits (1933), Expect = 2.0e-216
Identity = 380/477 (79.66%), Postives = 410/477 (85.95%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGA-SVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVA 60
           MA AVST GA+NR P+SLNG+ SGA S  +S FLG  +    SR   S   S  S KV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED    KQT+ DRW+GLA D SDDQQDITRGKGMVD VFQAP G GTH  VLSSYEY+
Sbjct: 61  VKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYV 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLRQYNLDN +DGFYIAPAFMDKLVVHI+KNF+ LPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGAG
Sbjct: 181 ELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPI+ TGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC GIFR+D +  EDI+ LVD FPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR++  S+GVE I K+LVNS+EGPP FEQP+M  EKL+EYG MLV EQENVKRVQLA+ 
Sbjct: 361 EVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAET 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQVSFPVPEGCTDPNAENFDPTARSDDGSCNY 477
           YLS+AALGDAN DAI  G FYGK AQQV+ PVPEGCTDP AENFDPTARSDDG+C Y
Sbjct: 421 YLSQAALGDANADAIGRGTFYGKGAQQVNLPVPEGCTDPVAENFDPTARSDDGTCVY 472

BLAST of Lcy09g001540 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 691.4 bits (1783), Expect = 5.0e-199
Identity = 352/447 (78.75%), Postives = 382/447 (85.46%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGA-SVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVA 60
           MA AVST GA+NR P+SLNG+ SGA S  +S FLG  +    SR   S   S  S KV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED    KQT+ DRW+GLA D SDDQQDITRGKGMVD VFQAP G GTH  VLSSYEY+
Sbjct: 61  VKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYV 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLRQYNLDN +DGFYIAPAFMDKLVVHI+KNF+ LPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGAG
Sbjct: 181 ELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPI+ TGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC GIFR+D +  EDI+ LVD FPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR++  S+GVE I K+LVNS+EGPP FEQP+M  EKL+EYG MLV EQENVKRVQLA+ 
Sbjct: 361 EVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAET 420

Query: 421 YLSEAALGDANEDAIKSGAFYGKAAQQ 447
           YLS+AALGDAN DAI  G FYGK  ++
Sbjct: 421 YLSQAALGDANADAIGRGTFYGKTEEK 442

BLAST of Lcy09g001540 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 690.6 bits (1781), Expect = 8.6e-199
Identity = 352/443 (79.46%), Postives = 380/443 (85.78%), Query Frame = 0

Query: 1   MATAVSTAGAVNRIPVSLNGAISGA-SVQSSAFLGSSLKKASSRLPNSKISSGSSLKVVA 60
           MA AVST GA+NR P+SLNG+ SGA S  +S FLG  +    SR   S   S  S KV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  VVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPAGQGTHDPVLSSYEYL 120
           V ED    KQT+ DRW+GLA D SDDQQDITRGKGMVD VFQAP G GTH  VLSSYEY+
Sbjct: 61  VKED----KQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYV 120

Query: 121 SAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLPNIKVPLILGVWGGKGQGKSFQC 180
           S GLRQYNLDN +DGFYIAPAFMDKLVVHI+KNF+ LPNIKVPLILG+WGGKGQGKSFQC
Sbjct: 121 SQGLRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQC 180

Query: 181 ELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCVLFINDLDAGAG 240
           ELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMC LFINDLDAGAG
Sbjct: 181 ELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAG 240

Query: 241 RLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIVVTGNDFSTLYAPLI 300
           R+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPI+ TGNDFSTLYAPLI
Sbjct: 241 RMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLI 300

Query: 301 RDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTFPGQSIDFFGALRARVYDD 360
           RDGRMEKFYWAPTREDRIGVC GIFR+D +  EDI+ LVD FPGQSIDFFGALRARVYDD
Sbjct: 301 RDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDD 360

Query: 361 EVRRWAASVGVENIAKKLVNSKEGPPKFEQPKMNLEKLLEYGYMLVQEQENVKRVQLADK 420
           EVR++  S+GVE I K+LVNS+EGPP FEQP+M  EKL+EYG MLV EQENVKRVQLA+ 
Sbjct: 361 EVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAET 420

Query: 421 YLSEAALGDANEDAIKSGAFYGK 443
           YLS+AALGDAN DAI  G FYGK
Sbjct: 421 YLSQAALGDANADAIGRGTFYGK 438

BLAST of Lcy09g001540 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 2.1e-88
Identity = 175/383 (45.69%), Postives = 246/383 (64.23%), Query Frame = 0

Query: 45  PNSKISSGSSLKVVAVVEDVDEKKQTNKDRWKGLASDISDDQQDITRGKGMVDPVFQAPA 104
           P  K+S  SS +V         K    KD    L ++  +    +    GM+D VF    
Sbjct: 60  PRKKLSEQSSWEV---------KDSEGKDYLYRLGAESDNVNIAVGARSGMIDDVFIGDF 119

Query: 105 GQGTHDPVLSSYEYLSAGLRQYNLDNNVDGFYIAPAFMDKLVVHISKNFMKLP-NIKVPL 164
                D V   ++Y     R +  ++    +YIAP+F+DK+ VHI KN++    NIK+PL
Sbjct: 120 LGKDSDIV---FDYRQKATRSF--EHLQGDYYIAPSFLDKVAVHIVKNYLAPSLNIKIPL 179

Query: 165 ILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIK- 224
           ILG+WGGKGQGK+FQ EL+F  MG+ P++MSAGELES  AGEP +LIR RYR A+ +I+ 
Sbjct: 180 ILGIWGGKGQGKTFQTELIFKTMGVEPVIMSAGELESDRAGEPGRLIRDRYRTASQVIQN 239

Query: 225 KGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN-PR 284
           +GKM VL IND+DAG GR  G TQ TVNNQ+V  TLMN+ADNPT V +   + + +   R
Sbjct: 240 QGKMSVLMINDIDAGLGRF-GETQMTVNNQIVVGTLMNLADNPTRVSVGQEWREADMVNR 299

Query: 285 VPIVVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCTGIFRSDNVAKEDIIKLVDTF 344
           VP++VTGNDFSTLYAPLIR+GRMEKFYW PTRED + + + ++  D ++++D+I +VD F
Sbjct: 300 VPLIVTGNDFSTLYAPLIREGRMEKFYWQPTREDIVNIVSRMYEKDGISRKDVISIVDKF 359

Query: 345 PGQSIDFFGALRARVYDDEVRRWAASV-GVENIAKKLVNSK--EGPPKFEQPKMNLEKLL 404
           P Q++DF+GALR+R YD  + +W     G+E + K L+  K  +  P+F  P+  +E LL
Sbjct: 360 PNQALDFYGALRSRTYDRSILKWVDEAGGMETLGKVLLRRKKTQEVPQFTAPEQTVEALL 419

Query: 405 EYGYMLVQEQENVKRVQLADKYL 422
           E GY L+ EQ+ +   +L+ +Y+
Sbjct: 420 ESGYSLINEQKLIMETKLSKEYM 427

BLAST of Lcy09g001540 vs. TAIR 10
Match: AT2G03670.1 (cell division cycle 48B )

HSP 1 Score: 50.1 bits (118), Expect = 5.9e-06
Identity = 41/163 (25.15%), Postives = 72/163 (44.17%), Query Frame = 0

Query: 159 IKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAA 218
           +K P  L ++G  G GK+     V  +   + I++S   +   +AGE  K++R+ + EA+
Sbjct: 53  LKWPRGLLLYGPPGTGKTSLVRAVVQECDAHLIVLSPHSVHRAHAGESEKVLREAFAEAS 112

Query: 219 DIIKKGKMCVLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE 278
                 K  V+FI+++D    R     +  V       TLM+ ++ P++           
Sbjct: 113 SHAVSDKPSVIFIDEIDVLCPRRDARREQDVRIASQLFTLMD-SNKPSS----------S 172

Query: 279 NPRVPIVVTGNDFSTLYAPLIRDGRMEKF--YWAPTREDRIGV 320
            PRV +V + N    +   L R GR +       P  EDR+ +
Sbjct: 173 APRVVVVASTNRVDAIDPALRRAGRFDALVEVSTPNEEDRLKI 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7X9A03.2e-22281.46Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea ... [more]
P108962.9e-21579.66Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Arabidops... [more]
Q402811.9e-21484.35Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus dom... [more]
P934316.0e-21380.72Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sat... [more]
P108718.1e-21075.37Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Spinacia ... [more]
Match NameE-valueIdentityDescription
A0A6J1JKS44.1e-25793.51ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita... [more]
A0A6J1GTI74.1e-25793.51ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita... [more]
A0A6J1FKE04.0e-25292.07ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucu... [more]
A0A0A0KBG22.0e-25191.35ATPase_AAA_core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G00... [more]
A0A6J1JQC92.8e-25091.98ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucu... [more]
Match NameE-valueIdentityDescription
XP_022955346.18.5e-25793.51ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita m... [more]
XP_022991087.18.5e-25793.51ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita m... [more]
XP_038886619.11.8e-25493.11ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Benincasa h... [more]
XP_023550769.12.8e-25292.28ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurb... [more]
XP_022938660.18.2e-25292.07ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurb... [more]
Match NameE-valueIdentityDescription
AT2G39730.12.0e-21679.66rubisco activase [more]
AT2G39730.25.0e-19978.75rubisco activase [more]
AT2G39730.38.6e-19979.46rubisco activase [more]
AT1G73110.12.1e-8845.69P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G03670.15.9e-0625.15cell division cycle 48B [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 127..311
e-value: 2.4E-43
score: 149.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 135..378
NoneNo IPR availableGENE3D1.10.8.1070coord: 312..424
e-value: 1.7E-50
score: 171.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..478
NoneNo IPR availablePANTHERPTHR32429:SF25RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 1..477
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 167..308
e-value: 1.1E-14
score: 55.0
IPR044960Ribulose bisphosphate carboxylase/oxygenase activase-likePANTHERPTHR32429FAMILY NOT NAMEDcoord: 1..477

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g001540.1Lcy09g001540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046863 ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity