Lcy09g001280 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g001280
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calcium-binding EF-hand family protein
Location	Chr09: 1117890 .. 1119292 (-)
RNA-Seq Expression	Lcy09g001280
Synteny	Lcy09g001280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGCCGTGTAAATAATAAAAGGCTAAGAGGGAAGAGTCCAAGACATGGTGACCACCTACTTATTTAATATCATTTGAGTTATCTTAGCAACCAAATGTAGTAAGGTCAAACAGTTATTTTTTGACTGTTTTACCTTATTTTTTCAGCGTCAAATTCAATACAATGTTATTTTTTCATAAAATTGAAAGTTTCATCCTCCATGCCCACATCTTCGCTTAGCTATGAATAATTTTTTTAAAACTTTTGAAATGTATCAATCAAAGAGAAGTTTTTCCACAAACAAAAAAAAAACAAGAAAGTTTTCCCTTTTATTATTTGATTTTTTCAAAGAGAGGGAGGAAAGTATCGGACCTGTTGCTAGTTGCCATTTGCCAACCGTTTTAGACAGAAAGAAATGGAAACGCAGACGAAAAGCGGAGAGTTTACGAAGACTCTTTCTTCTTCAGGTTTTACATGATTTTCTTCTTCGCAGAATTAGGGCTTTTTCCACCTTTTCTAGACTCTTGACTGATTAATCAATCCAACCAGCATTCCTTCCTTCCTTCTTCTGTCAATCTTTGACGCATCATTGTTTAATCAACAATCGCTGAGAAAGCCCTCGTTCAACTTCAAGAAATGGTCGACCACCTGCTCCACGAGTTGACGGCGACCGCCATTTTCATTTACCAACTTCGTCGCCAAATCCGGCGGTCAAACCACGGTACGCGCATTCCCTTTCATATAATTGCAGAACCCATTTCGCCCTAATCCCCAATTCTTCATTCTCATTTCTCTTCCTTCACTCTCGCTCTCCCCGCATTCAGAATTATGGACAAAACCGCATTGTACGAGAAGGTGTTCGAGCAGGTCGACGGCGACGGCGACGGGAAGCTGTCTCCGCCGGAGCTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGCAGGAGGCGGAGATGGTGGTGAAGAATTTGGATTCCGATGGCGATGGGTTGGTGGGATGGGCTGAATTTGTTGCGTTTGTGGAAGGAGTGGAAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGCTGTGGCTTCATAACGACCAAGAGTTTGAAGAGAATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAAGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCTTAACTTTGATGAGTTCAGTTGTTATATGATTTACTCTTTTTCTTTTGTTCTTCTTGAATGGTTTGCATTCTCTCTCTCTCTCTTGACATGTATCTTTTTGGTCTCCCTCTCTTCCCATTCAATTGGAAGTGAAAAATAGTGGTTTGAAATCCTTGCTATATTGATTAACTTTGTAA

mRNA sequence

GGGCCGTGTAAATAATAAAAGGCTAAGAGGGAAGAGTCCAAGACATGGTGACCACCTACTTATTTAATATCATTTGAGTTATCTTAGCAACCAAATGTAGTAAGGTCAAACAGTTATTTTTTGACTGTTTTACCTTATTTTTTCAGCGTCAAATTCAATACAATGTTATTTTTTCATAAAATTGAAAGTTTCATCCTCCATGCCCACATCTTCGCTTAGCTATGAATAATTTTTTTAAAACTTTTGAAATGTATCAATCAAAGAGAAGTTTTTCCACAAACAAAAAAAAAACAAGAAAGTTTTCCCTTTTATTATTTGATTTTTTCAAAGAGAGGGAGGAAAGTATCGGACCTGTTGCTAGTTGCCATTTGCCAACCGTTTTAGACAGAAAGAAATGGAAACGCAGACGAAAAGCGGAGAGTTTACGAAGACTCTTTCTTCTTCAGCATTCCTTCCTTCCTTCTTCTGTCAATCTTTGACGCATCATTGTTTAATCAACAATCGCTGAGAAAGCCCTCGTTCAACTTCAAGAAATGGTCGACCACCTGCTCCACGAGTTGACGGCGACCGCCATTTTCATTTACCAACTTCGTCGCCAAATCCGGCGGTCAAACCACGAATTATGGACAAAACCGCATTGTACGAGAAGGTGTTCGAGCAGGTCGACGGCGACGGCGACGGGAAGCTGTCTCCGCCGGAGCTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGCAGGAGGCGGAGATGGTGGTGAAGAATTTGGATTCCGATGGCGATGGGTTGGTGGGATGGGCTGAATTTGTTGCGTTTGTGGAAGGAGTGGAAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGCTGTGGCTTCATAACGACCAAGAGTTTGAAGAGAATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAAGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCTTAACTTTGATGAGTTCAGTTGTTATATGATTTACTCTTTTTCTTTTGTTCTTCTTGAATGGTTTGCATTCTCTCTCTCTCTCTTGACATGTATCTTTTTGGTCTCCCTCTCTTCCCATTCAATTGGAAGTGAAAAATAGTGGTTTGAAATCCTTGCTATATTGATTAACTTTGTAA

Coding sequence (CDS)

ATGGACAAAACCGCATTGTACGAGAAGGTGTTCGAGCAGGTCGACGGCGACGGCGACGGGAAGCTGTCTCCGCCGGAGCTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGCAGGAGGCGGAGATGGTGGTGAAGAATTTGGATTCCGATGGCGATGGGTTGGTGGGATGGGCTGAATTTGTTGCGTTTGTGGAAGGAGTGGAAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGCTGTGGCTTCATAACGACCAAGAGTTTGAAGAGAATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAAGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCTTAA

Protein sequence

MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS

Homology

[Close]

BLAST of Lcy09g001280 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.7e-30
Identity = 72/140 (51.43%), Postives = 96/140 (68.57%), Query Frame = 0


Query: 4   TALYEKVFEQVDGDGDGKLSPPELQRCM-AAVGGSLTLQEAEMVVKNLDSDGDGLVGWAE 63
           TA + +VF   D D DGK+S  EL+ CM AA+G  ++ +EAE +V + D+D DGL+   E
Sbjct: 5   TAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEE 64

Query: 64  FVAF---VEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI 123
           F      +E  +EEE+   L EAFRMYEM+G G IT  SLKRMLS+LG  + IEEC+ MI
Sbjct: 65  FTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMI 124

Query: 124 AKFDLNSDGVLNFDEFKFMM 140
            +FDL+ DGV++F+EFK MM
Sbjct: 125 CRFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of Lcy09g001280 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.7e-30
Identity = 69/132 (52.27%), Postives = 93/132 (70.45%), Query Frame = 0


Query: 10  VFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEG 69
           VF+ +D + DGK+S  ELQ C++ +GG+L+ +E E VVK  D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  VE--EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
            +  +EE+  +LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of Lcy09g001280 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.2e-29
Identity = 65/138 (47.10%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 2   DKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ +EA   V+  D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++  +EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Lcy09g001280 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-28
Identity = 68/136 (50.00%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 10  VFEQVDGDGDGKLSPPELQRCM-AAVGGSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVE 69
           +F   D DGDG++S  EL+ CM   +G  ++ +EA  +V ++D+DGDGL+  AEFV  V+
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  GVEEEE----KVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 129
             E EE    + + L+EAF MYEM+G G IT  SL+RML RLG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NSDGVLNFDEFKFMMS 141
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

[Close]

BLAST of Lcy09g001280 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.3e-26
Identity = 66/141 (46.81%), Postives = 89/141 (63.12%), Query Frame = 0


Query: 2   DKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWA 61
           +K    E VF  +D + DG++S  EL++    +G  ++ +EAE  VK  D DGDG++   
Sbjct: 18  EKNRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDIN 77

Query: 62  EFVAFVEGVE---EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKM 121
           EF   ++G +   EEEK   + EAFRMY  DG   IT  SLK ML +LGESR+ ++CK M
Sbjct: 78  EFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVM 137

Query: 122 IAKFDLNSDGVLNFDEFKFMM 140
           I  FDLN+DGVL+FDEF  MM
Sbjct: 138 IQAFDLNADGVLSFDEFALMM 158

[Close]

BLAST of Lcy09g001280 vs. ExPASy TrEMBL
Match: A0A6J1BWC8 (putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC111006309 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 8.0e-68
Identity = 132/140 (94.29%), Postives = 136/140 (97.14%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF QVDGDGDGKLSPPELQ+CMAAVGGSLTL+EAE+VV NLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAFVEGV+EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lcy09g001280 vs. ExPASy TrEMBL
Match: A0A6J1GWQ9 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111457555 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.8e-65
Identity = 129/140 (92.14%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLS PELQRCMA VGGSLTL+EAE VV+NLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAF+EGV EEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lcy09g001280 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.8e-65
Identity = 128/140 (91.43%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLSP ELQRCMA VGGSLTL+EAEMVV+NLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVEGV EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of Lcy09g001280 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.7e-65
Identity = 128/140 (91.43%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLSP ELQRCMA VGGSLTL+EAEM V+NLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVE V EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

[Close]

BLAST of Lcy09g001280 vs. ExPASy TrEMBL
Match: A0A6J1JS06 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111487817 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.4e-64
Identity = 128/140 (91.43%), Postives = 132/140 (94.29%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLS PELQRCMA VGGSLTL+EAE VV+NLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAF+E V EEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lcy09g001280 vs. NCBI nr
Match: XP_022133861.1 (putative calcium-binding protein CML19 [Momordica charantia])

HSP 1 Score: 266.2 bits (679), Expect = 1.6e-67
Identity = 132/140 (94.29%), Postives = 136/140 (97.14%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF QVDGDGDGKLSPPELQ+CMAAVGGSLTL+EAE+VV NLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAFVEGV+EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lcy09g001280 vs. NCBI nr
Match: XP_022955599.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 putative calcium-binding protein CML19 [Cucurbita moschata] >KAG6602674.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033361.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 257.7 bits (657), Expect = 5.8e-65
Identity = 129/140 (92.14%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLS PELQRCMA VGGSLTL+EAE VV+NLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAF+EGV EEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lcy09g001280 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 257.7 bits (657), Expect = 5.8e-65
Identity = 128/140 (91.43%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLSP ELQRCMA VGGSLTL+EAEMVV+NLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVEGV EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of Lcy09g001280 vs. NCBI nr
Match: KAG6578374.1 (Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 257.7 bits (657), Expect = 5.8e-65
Identity = 128/140 (91.43%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1    MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
            MDK ALYEKVF+QVDGDGDGKLSP ELQRCMA VGGSLTL+EAEMVV+NLD+DGDGL+GW
Sbjct: 1309 MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 1368

Query: 61   AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
             EFVAFVEGV EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 1369 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 1428

Query: 121  KFDLNSDGVLNFDEFKFMMS 141
            KFDLNSDGVLNFDEF FMMS
Sbjct: 1429 KFDLNSDGVLNFDEFMFMMS 1448

[Close]

BLAST of Lcy09g001280 vs. NCBI nr
Match: XP_023550775.1 (putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 257.7 bits (657), Expect = 5.8e-65
Identity = 128/140 (91.43%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGW 60
           MDK ALYEKVF+QVDGDGDGKLSP ELQRCMA VGGSLTL+EAEMVV+NLD+DGDGL+GW
Sbjct: 58  MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 117

Query: 61  AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVEGV EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 118 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 177

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 178 KFDLNSDGVLNFDEFMFMMS 197

[Close]

BLAST of Lcy09g001280 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-31
Identity = 69/132 (52.27%), Postives = 93/132 (70.45%), Query Frame = 0


Query: 10  VFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEG 69
           VF+ +D + DGK+S  ELQ C++ +GG+L+ +E E VVK  D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  VE--EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
            +  +EE+  +LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of Lcy09g001280 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 127.9 bits (320), Expect = 6.5e-30
Identity = 65/138 (47.10%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 2   DKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ +EA   V+  D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++  +EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Lcy09g001280 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 127.9 bits (320), Expect = 6.5e-30
Identity = 65/138 (47.10%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 2   DKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ +EA   V+  D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++  +EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Lcy09g001280 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.3e-27
Identity = 66/141 (46.81%), Postives = 89/141 (63.12%), Query Frame = 0


Query: 2   DKTALYEKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWA 61
           +K    E VF  +D + DG++S  EL++    +G  ++ +EAE  VK  D DGDG++   
Sbjct: 18  EKNRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDIN 77

Query: 62  EFVAFVEGVE---EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKM 121
           EF   ++G +   EEEK   + EAFRMY  DG   IT  SLK ML +LGESR+ ++CK M
Sbjct: 78  EFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVM 137

Query: 122 IAKFDLNSDGVLNFDEFKFMM 140
           I  FDLN+DGVL+FDEF  MM
Sbjct: 138 IQAFDLNADGVLSFDEFALMM 158

[Close]

BLAST of Lcy09g001280 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 117.1 bits (292), Expect = 1.1e-26
Identity = 60/138 (43.48%), Postives = 89/138 (64.49%), Query Frame = 0


Query: 8   EKVFEQVDGDGDGKLSPPELQRCMAAVGGSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFV 67
           +KVF++ D +GDGK+S  EL+  + A+  + + +E   ++K  D DG+G +   EFVA  
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  E-----GVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKF 127
           +     G      VSDLKEAF +Y++DG G I+ K L  ++  LGE  S+++CKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLNSDGVLNFDEFKFMMS 141
           D++ DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

The following BLAST results are available for this feature:

BLAST of Lcy09g001280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYJ8	1.7e-30	51.43	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FIH9	1.7e-30	52.27	Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1	[more]
Q9SRE6	9.2e-29	47.10	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYK0	1.2e-28	50.00	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE7	3.3e-26	46.81	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]

BLAST of Lcy09g001280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWC8	8.0e-68	94.29	putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1GWQ9	2.8e-65	92.14	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1FET5	2.8e-65	91.43	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1JZS3	3.7e-65	91.43	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889...	[more]
A0A6J1JS06	2.4e-64	91.43	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114878...	[more]

BLAST of Lcy09g001280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133861.1	1.6e-67	94.29	putative calcium-binding protein CML19 [Momordica charantia]	[more]
XP_022955599.1	5.8e-65	92.14	putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 puta...	[more]
XP_022938684.1	5.8e-65	91.43	putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 puta...	[more]
KAG6578374.1	5.8e-65	91.43	Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023550775.1	5.8e-65	91.43	putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy09g001280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42380.1	1.2e-31	52.27	calmodulin like 37	[more]
AT1G76650.1	6.5e-30	47.10	calmodulin-like 38	[more]
AT1G76650.3	6.5e-30	47.10	calmodulin-like 38	[more]
AT1G76640.1	2.3e-27	46.81	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	1.1e-26	43.48	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 78..106 e-value: 0.28 score: 20.2 coord: 6..34 e-value: 0.0057 score: 25.9 coord: 114..140 e-value: 0.26 score: 20.3 coord: 42..70 e-value: 12.0 score: 9.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 4..67 e-value: 1.1E-12 score: 48.2 coord: 78..139 e-value: 3.1E-11 score: 43.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 11.444912
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 38..73 score: 10.524363
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 2..37 score: 12.672309
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..140 score: 14.318138
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 78..140 e-value: 1.7256E-15 score: 64.4913
None	No IPR available	GENE3D	1.10.238.10		coord: 1..72 e-value: 1.1E-15 score: 59.6
None	No IPR available	GENE3D	1.10.238.10		coord: 73..140 e-value: 1.1E-19 score: 72.4
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..140
None	No IPR available	PANTHER	PTHR10891:SF858	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 2..140
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 51..63
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 15..27
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 7..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g001280.1	Lcy09g001280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0016301	kinase activity