Lcy08g018600 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g018600
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionglutathione hydrolase 3-like
LocationChr08: 49374698 .. 49378543 (-)
RNA-Seq ExpressionLcy08g018600
SyntenyLcy08g018600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTTGCGTCTTGGATAAGTATCTTATATAGAGTCTGCGATTCTTCGATAATTTTTCTCTGTTCATCGAGCGGATCTGTGGAATGAACCATGGAATCTCCATTGTTGCGCAGCCATGAATTGGTGGGGAAGTGGAGTTGCAGACGAGTGATTCTTATTCCGCTCGGATTACTAGCCATCACAGGTGCTTTATCTGATCTTCACCTTTGTTTATCTTGTCTTATTGTATACTTTCGAGTTTCTCCCGAATTCAGGAACAGTGATTATTCATCTGAGGGTAATTGTTCTAAGAAACCAATGATTATGGGTTTTATTCAGGATGTTCCACAGTTCTTTTGTTTTATGATGATTCTGACTTTGTTTTAGTTCTTTGATGGTCTTGAATGATTCCGGCAGTTGAATTGAGACCTTCCTTTTTGATTTCGAGGCTTTTGTTTATTGATGTTGATTTTGACTGGAAGATGAGCTTACCTGCCCGCCCCTTTTACTTGAGTTGAATTGGTTCTTATTACCTGAAAATCTGCCGACTATGTTGACTTTGTTTGATAACTTTTGGTTTTTGGTTGTATGTATTTGAAATTTGAGCTTATGAACTCTACTTCGATTTGTGTATTTCTTTATTTTGGTATTTACTTTTTAGAAACATTTTCAAAATCTAACCCGAAATTTGGAAGTTAAAAGAAATATGGCTTTTGAAAATTTGGTTAAGAATTCAAAAATGTTTTTACAAAAGGTAGAAATATATATACCAAAGTAATGGTGTGAAAGCAATGAAAATAGAAAACTAAAAACGAAATCGTTATCCATGGGGGCCTTCAACGTTAAGCTATAGTTATAGACAACTTACAAGAGAAAAACAAGTTGCAACCAAGTACAGTTATTATCTTGCTTCTTTGCTGCTAGGCACAACAGATTAAGGTTTAAAAGATTGTAAAATGGTATCACTAACTGATGAAGTCTGACCCTTAAAGCTGGGGAATGGTTTTCATTTTGTAGTTGTTGGCTACATAATTGGAGGTTCCATAAATGGTTCAGTATTTCATGAAGAAAACAAGCCCTTTGATGGAGTACATACCGATAATCCCGAAATTGTTGAGTCAGAAAAGGGAGTTGTTGCTGCTGATGATGGCCGCTGCTCCGAAATTGGAGCGTCGATGCTTAGGCGAGGAGGGCATGCTGTTGATGCTGCAGTTGCAACTGCATTGTGCCTCGGTGTGGTGTGTTCGATGTCAAGCGGGATAGGTGGTGGAGGTTTTATGCTTGTGCGATCCGGGTTGACTTTGCAAACCATAGCTCTTGATTTCCGGGAGACTGCACCCTTGGCTGCCTCTCAGGTTCAAAATCTCTCTCTTTTTGTTCAAGAAGTCAAAAGTACTGTGTAGAAGATGAAGGTGGAGGGTTGAGTCCAATGGAACAAGCAGAGTGAAAAGTTGTAAGATATTTCTCTTTGGCAATGTGCAGGATATGTATGAAACCAATGTGACAGCCAAACATGTTGGGCCATTATCAGTTGCAGTCCCTGGAGAGATAGCTGGCCTTCATGAAGCTTGGTTGAGATATGGCCGTTTGGCTTGGAAGTCCTTATTCGAACCTGCTATAAAGATTGCCAAAGATGGATTTGTGATTTCACCTTTTCTAGGAAAGTCCTTAGTTTCCTCTGCTAAGCTGATACTAAATGACCCTGGGTTGAGACAAGTTTTCGCACCAAATGGTGACCTATTACAAACAGGCGATACTTGCTATAATGTCGAACTAGGAAAGAGTTTAGAGGCAGTGGCAGATCAAGGACCACAAGTCTTCTATAACGGAATCGTCGGAGAGAAGTTGGTGAAGGATGTGAGAGCAGTTGGTGGCATTTTATCCATGGATGATTTGAAGAACTACACAGTGGAAGTAGCCGAAGCAATGAATACGGAGGCAATGGGCTACACAGTGCATGGCATGCCACCCCCATCAAGTGGAACACTTGGATTTGCTATGGTCTTGTCCTCTCTCTACTTCCTAATGGAACTTTTGTTTTTGGATTGAGAGTCCTTTATGTGAGAACGATAATGATTTGAGAATCTATTCTTGAATGTTCAATCTTGGGAAACAGGTAATGAATATCTTTAAAAGCTACAACGATCCTGATGCTGCTAAAGGAAATATTGGCCTACATCGCCTAATTGAAGCATTGAAGCACATGTATGCTGAGCGAATGAACTTGGGTGATCCTCGTTTTTCCAACATTAGCAACTCTGTCTCCAACATGCTCAGTCCATCATTTGCAAAGAAAATTCAGGAAAAGATAATTGACAATACTACTTTTCCTCCAGATTACTATCTATACAGGTATCTACATAAGCAAAGTATGGCTAGCTATGCCTACAATTGAGAGTGGGAATTTGAACCTTCAACTTATAAAGGAAGCTCATGCTTGCTAATTTTACTTGTTATTGCAAATAAATTCATGACACAAACTTGTTCTTCACAATTACTAGGTGGAGTCAGCTAAGAGATCATGGAACCAGTCATTTTTGCGTTGTGGATGCAGATAGAAATGCCGTATCATTGACAACAACTGTAAATGAGCACTTTGGTGCTGGCATTCTCTCACCTTCTACTGGTATCGTTCTCAATAATGAGATGGGGGACTTCTCTATTCCCACAGATATATCTCCAGATAAACTTCCTCCAGCTCCAGCAAATTTTATCCAGCCAAACAAGAGGCCTCTCTCTTCTATGACTCCTATTATCGTCACAAAGGTTTCGTTTTCATTTATATATTTGACTGGTTTTGATCTTTTTGTTACTCTATTAATCCACTTTAGATGTGACTACGTTGCAGATAGATTATGATATATTTAATCTTTAATATATATTCTTAACAATGACCTTGCTCTGATATTACATTAAACTACCACCAAACTCAAAAACTTAAATGGATAAGTTTTGATATATTTAATCTTTTATACATATTCTTAACATTTTGAACGTGCATCGAACTATACTCTTCAATCAATCATCAATAATTTACCTTGGAAATGTTGTTTGAACCTTCATAGGATGATCGGTTGATTGCGGTAATTGGAGGTAGCGGTGGTATGAAGATAATTCCTGCAGTAATTGAGGTTTTCCTTAATTATTTTTCCCTGGGATTGCAACCTTTAGCAGCAGTTCAAAGGTCAAGGGTGTATCATCAGGTTTGCTACTCAGTTTCCATACATAACTTATTATCACAAACAGTTGACATTGTAATGTAGCCTTGTTTTCTCTCTGCAGTTAATTCCAAATGTAGTTAAATACGAAAACTTGACGTTCATCGATGGCGATCACGTCGAACTTCCAGACGAAAAGAAGCAATTCTTGGAAGATAGGGGTCATCAAGTGGTAGCTATTGATACAGCAGGAGCCATTGTCCAGTTCATAGTTCAAAACTTTGAAGAAGCCATTGACACAGGTCGAAAGGGCGGAAAGATATCCAATAACCAATCACTTTTCGGTCTCCTTACGGCTGTCTGCGACCCGAGAAAGAACGGATTTCCTGCAGTTGTTTAGCTTTCTAGCTTTGGAGAACAATGGGAAAGGATGCAAGCTCTGTTTGTGATTGTTTCCCATCTCCATCTGTTAGATAATATTGCCTCCTCTTGTCCATGTATTGATTTATTTGACCATGGAAATGTTTATTGTTTACTTGGACTGTTCTTAAATGAGGCATAGCTATCTCTCAGTTGGTTGAATGATCAGTTTCCTTGAGTTTCAAGATGGACATCAATAGGGAAATGGGGATCAAAAGAAGAGAACAAGAAGGAAAATAAGGTCTTTGAGAATTGCTTTTAGAAATTTCTTCAATGCCTGGTAATTCAAG

mRNA sequence

GCCTTGCGTCTTGGATAAGTATCTTATATAGAGTCTGCGATTCTTCGATAATTTTTCTCTGTTCATCGAGCGGATCTGTGGAATGAACCATGGAATCTCCATTGTTGCGCAGCCATGAATTGGTGGGGAAGTGGAGTTGCAGACGAGTGATTCTTATTCCGCTCGGATTACTAGCCATCACAGTTGTTGGCTACATAATTGGAGGTTCCATAAATGGTTCAGTATTTCATGAAGAAAACAAGCCCTTTGATGGAGTACATACCGATAATCCCGAAATTGTTGAGTCAGAAAAGGGAGTTGTTGCTGCTGATGATGGCCGCTGCTCCGAAATTGGAGCGTCGATGCTTAGGCGAGGAGGGCATGCTGTTGATGCTGCAGTTGCAACTGCATTGTGCCTCGGTGTGGTGTGTTCGATGTCAAGCGGGATAGGTGGTGGAGGTTTTATGCTTGTGCGATCCGGGTTGACTTTGCAAACCATAGCTCTTGATTTCCGGGAGACTGCACCCTTGGCTGCCTCTCAGGATATGTATGAAACCAATGTGACAGCCAAACATGTTGGGCCATTATCAGTTGCAGTCCCTGGAGAGATAGCTGGCCTTCATGAAGCTTGGTTGAGATATGGCCGTTTGGCTTGGAAGTCCTTATTCGAACCTGCTATAAAGATTGCCAAAGATGGATTTGTGATTTCACCTTTTCTAGGAAAGTCCTTAGTTTCCTCTGCTAAGCTGATACTAAATGACCCTGGGTTGAGACAAGTTTTCGCACCAAATGGTGACCTATTACAAACAGGCGATACTTGCTATAATGTCGAACTAGGAAAGAGTTTAGAGGCAGTGGCAGATCAAGGACCACAAGTCTTCTATAACGGAATCGTCGGAGAGAAGTTGGTGAAGGATGTGAGAGCAGTTGGTGGCATTTTATCCATGGATGATTTGAAGAACTACACAGTGGAAGTAGCCGAAGCAATGAATACGGAGGCAATGGGCTACACAGTGCATGGCATGCCACCCCCATCAAGTGGAACACTTGGATTTGCTATGGTAATGAATATCTTTAAAAGCTACAACGATCCTGATGCTGCTAAAGGAAATATTGGCCTACATCGCCTAATTGAAGCATTGAAGCACATGTATGCTGAGCGAATGAACTTGGGTGATCCTCGTTTTTCCAACATTAGCAACTCTGTCTCCAACATGCTCAGTCCATCATTTGCAAAGAAAATTCAGGAAAAGATAATTGACAATACTACTTTTCCTCCAGATTACTATCTATACAGGTGGAGTCAGCTAAGAGATCATGGAACCAGTCATTTTTGCGTTGTGGATGCAGATAGAAATGCCGTATCATTGACAACAACTGTAAATGAGCACTTTGGTGCTGGCATTCTCTCACCTTCTACTGGTATCGTTCTCAATAATGAGATGGGGGACTTCTCTATTCCCACAGATATATCTCCAGATAAACTTCCTCCAGCTCCAGCAAATTTTATCCAGCCAAACAAGAGGCCTCTCTCTTCTATGACTCCTATTATCGTCACAAAGGATGATCGGTTGATTGCGGTAATTGGAGGTAGCGGTGGTATGAAGATAATTCCTGCAGTAATTGAGGTTTTCCTTAATTATTTTTCCCTGGGATTGCAACCTTTAGCAGCAGTTCAAAGGTCAAGGGTGTATCATCAGTTAATTCCAAATGTAGTTAAATACGAAAACTTGACGTTCATCGATGGCGATCACGTCGAACTTCCAGACGAAAAGAAGCAATTCTTGGAAGATAGGGGTCATCAAGTGGTAGCTATTGATACAGCAGGAGCCATTGTCCAGTTCATAGTTCAAAACTTTGAAGAAGCCATTGACACAGGTCGAAAGGGCGGAAAGATATCCAATAACCAATCACTTTTCGGTCTCCTTACGGCTGTCTGCGACCCGAGAAAGAACGGATTTCCTGCAGTTGTTTAGCTTTCTAGCTTTGGAGAACAATGGGAAAGGATGCAAGCTCTGTTTGTGATTGTTTCCCATCTCCATCTGTTAGATAATATTGCCTCCTCTTGTCCATGTATTGATTTATTTGACCATGGAAATGTTTATTGTTTACTTGGACTGTTCTTAAATGAGGCATAGCTATCTCTCAGTTGGTTGAATGATCAGTTTCCTTGAGTTTCAAGATGGACATCAATAGGGAAATGGGGATCAAAAGAAGAGAACAAGAAGGAAAATAAGGTCTTTGAGAATTGCTTTTAGAAATTTCTTCAATGCCTGGTAATTCAAG

Coding sequence (CDS)

ATGGAATCTCCATTGTTGCGCAGCCATGAATTGGTGGGGAAGTGGAGTTGCAGACGAGTGATTCTTATTCCGCTCGGATTACTAGCCATCACAGTTGTTGGCTACATAATTGGAGGTTCCATAAATGGTTCAGTATTTCATGAAGAAAACAAGCCCTTTGATGGAGTACATACCGATAATCCCGAAATTGTTGAGTCAGAAAAGGGAGTTGTTGCTGCTGATGATGGCCGCTGCTCCGAAATTGGAGCGTCGATGCTTAGGCGAGGAGGGCATGCTGTTGATGCTGCAGTTGCAACTGCATTGTGCCTCGGTGTGGTGTGTTCGATGTCAAGCGGGATAGGTGGTGGAGGTTTTATGCTTGTGCGATCCGGGTTGACTTTGCAAACCATAGCTCTTGATTTCCGGGAGACTGCACCCTTGGCTGCCTCTCAGGATATGTATGAAACCAATGTGACAGCCAAACATGTTGGGCCATTATCAGTTGCAGTCCCTGGAGAGATAGCTGGCCTTCATGAAGCTTGGTTGAGATATGGCCGTTTGGCTTGGAAGTCCTTATTCGAACCTGCTATAAAGATTGCCAAAGATGGATTTGTGATTTCACCTTTTCTAGGAAAGTCCTTAGTTTCCTCTGCTAAGCTGATACTAAATGACCCTGGGTTGAGACAAGTTTTCGCACCAAATGGTGACCTATTACAAACAGGCGATACTTGCTATAATGTCGAACTAGGAAAGAGTTTAGAGGCAGTGGCAGATCAAGGACCACAAGTCTTCTATAACGGAATCGTCGGAGAGAAGTTGGTGAAGGATGTGAGAGCAGTTGGTGGCATTTTATCCATGGATGATTTGAAGAACTACACAGTGGAAGTAGCCGAAGCAATGAATACGGAGGCAATGGGCTACACAGTGCATGGCATGCCACCCCCATCAAGTGGAACACTTGGATTTGCTATGGTAATGAATATCTTTAAAAGCTACAACGATCCTGATGCTGCTAAAGGAAATATTGGCCTACATCGCCTAATTGAAGCATTGAAGCACATGTATGCTGAGCGAATGAACTTGGGTGATCCTCGTTTTTCCAACATTAGCAACTCTGTCTCCAACATGCTCAGTCCATCATTTGCAAAGAAAATTCAGGAAAAGATAATTGACAATACTACTTTTCCTCCAGATTACTATCTATACAGGTGGAGTCAGCTAAGAGATCATGGAACCAGTCATTTTTGCGTTGTGGATGCAGATAGAAATGCCGTATCATTGACAACAACTGTAAATGAGCACTTTGGTGCTGGCATTCTCTCACCTTCTACTGGTATCGTTCTCAATAATGAGATGGGGGACTTCTCTATTCCCACAGATATATCTCCAGATAAACTTCCTCCAGCTCCAGCAAATTTTATCCAGCCAAACAAGAGGCCTCTCTCTTCTATGACTCCTATTATCGTCACAAAGGATGATCGGTTGATTGCGGTAATTGGAGGTAGCGGTGGTATGAAGATAATTCCTGCAGTAATTGAGGTTTTCCTTAATTATTTTTCCCTGGGATTGCAACCTTTAGCAGCAGTTCAAAGGTCAAGGGTGTATCATCAGTTAATTCCAAATGTAGTTAAATACGAAAACTTGACGTTCATCGATGGCGATCACGTCGAACTTCCAGACGAAAAGAAGCAATTCTTGGAAGATAGGGGTCATCAAGTGGTAGCTATTGATACAGCAGGAGCCATTGTCCAGTTCATAGTTCAAAACTTTGAAGAAGCCATTGACACAGGTCGAAAGGGCGGAAAGATATCCAATAACCAATCACTTTTCGGTCTCCTTACGGCTGTCTGCGACCCGAGAAAGAACGGATTTCCTGCAGTTGTTTAG

Protein sequence

MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDNPEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLVRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLAWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYTVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFSNISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSLTTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPIIVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYENLTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQSLFGLLTAVCDPRKNGFPAVV
Homology
BLAST of Lcy08g018600 vs. ExPASy Swiss-Prot
Match: Q9M0G0 (Glutathione hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=GGT3 PE=2 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 7.8e-213
Identity = 360/567 (63.49%), Postives = 455/567 (80.25%), Query Frame = 0

Query: 62  EIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLV 121
           ++VESE GVVAADD RCSEIGAS+LR GGHAVDAAVA  LC+GVV  MSSGIGGG F++V
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRSGGHAVDAAVAITLCVGVVNPMSSGIGGGSFLIV 133

Query: 122 RSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLA 181
            S    +  A D RETAPLAAS+DMY+ + +AK +G LS+ VPGEIAGL+EAW RYGRL 
Sbjct: 134 SSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRLP 193

Query: 182 WKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVE 241
           WK LFEPAIK+A+DGFV+ P+LGK++ +   +IL DPG+R VF+ NG +L+TG+TCYN E
Sbjct: 194 WKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSRNGQVLKTGETCYNPE 253

Query: 242 LGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYT 301
           L +SLE +++QGP  FYNG VGEKLVKDV+  GGI++MDDL++Y V V +AM+ + MGYT
Sbjct: 254 LAQSLETISEQGPGAFYNGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAMSVDVMGYT 313

Query: 302 VHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKG-NIGLHRLIEALKHMYAERMNLGDPRFS 361
           VHGMPPPS GT+GF+MVMNI  SY++   A G  +GLHRLIEA+KHM+A RM+LGDP F 
Sbjct: 314 VHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMDLGDPEFV 373

Query: 362 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 421
           N++NS++ MLS + A++IQ++I DNTTFPP+YY+ RWSQLRD GTSHFCVVDADRN+VS+
Sbjct: 374 NVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDADRNSVSM 433

Query: 422 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 481
           T+TVN  FGAG+LSPSTGIVLNNEM DFS PT+I+PD LPPAP NFI+PNKRPLSSMTP+
Sbjct: 434 TSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRPLSSMTPL 493

Query: 482 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 541
           ++TKD   +A +GG+GGM IIPAV++VFLN F L ++P  AV+ +R+YH+LIPNVV YEN
Sbjct: 494 VITKDGEFVAALGGAGGMHIIPAVLQVFLNCFVLNMKPKEAVESARIYHRLIPNVVSYEN 553

Query: 542 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEE------AIDTGRKGG 601
            T I+GDH+ + ++ K FL +RGH++  + + GAIVQ IVQ+F+E       I+ GRK G
Sbjct: 554 FTTINGDHIGVSEDTKMFLAERGHELKEL-SGGAIVQLIVQSFKEEKEEEMIIEIGRKIG 613

Query: 602 KISNNQSLFGLLTAVCDPRKNGFPAVV 622
           K   ++ L GLLTAV DPRK+G PA V
Sbjct: 614 K--KSKPLKGLLTAVSDPRKDGKPAAV 637

BLAST of Lcy08g018600 vs. ExPASy Swiss-Prot
Match: Q680I5 (Glutathione hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=GGT2 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 2.3e-156
Identity = 284/563 (50.44%), Postives = 379/563 (67.32%), Query Frame = 0

Query: 60  NPEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFM 119
           N + + +  G VA DDGRCS IG ++LR+GG+A+DA+VA ALCLGVV   SSGIGGG F 
Sbjct: 29  NLQSIVAYHGAVATDDGRCSAIGTNVLRQGGNAIDASVAAALCLGVVSPASSGIGGGAFT 88

Query: 120 LVRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGR 179
           +++     + +A D RETAPL+A++DMY  N   K  G LSV VPGE+AGL+ AW ++G+
Sbjct: 89  MIKLANGTE-VAYDSRETAPLSATEDMYGDNPERKKKGSLSVGVPGEVAGLYTAWTQHGK 148

Query: 180 LAWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYN 239
           L WK L EPA K+A +GF IS +L   + ++   IL D GL ++F  NG+L + G  C N
Sbjct: 149 LPWKQLVEPAEKLAAEGFKISKYLYMQMNATRSDILADKGLSELFVSNGELKKPGAICRN 208

Query: 240 VELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMG 299
            +L  +L  +A+ GP+ FYNG VG  LV D++  GGI+++ DL+NY V+V E ++TE +G
Sbjct: 209 PKLADTLSQIAEYGPKAFYNGTVGFNLVSDIQKAGGIITLKDLQNYNVKVKEPLSTEILG 268

Query: 300 YTVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRF 359
           Y + GMPPPSSG     +++NI   Y  P    G +G+HRL+EALKH +A RMNLGDP F
Sbjct: 269 YRLLGMPPPSSGGPAMMLILNILAQYGIPSGVSGPLGVHRLVEALKHAFAVRMNLGDPDF 328

Query: 360 -SNISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAV 419
              ++N V++MLSP FA+ ++ KI D  TF P YY  +W Q++DHGTSH  ++D++RNAV
Sbjct: 329 VPEVTNVVADMLSPKFAQDLKSKINDEKTFDPKYYGGKWGQIKDHGTSHLSIIDSERNAV 388

Query: 420 SLTTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDI--SPDKLPPAPANFIQPNKRPLSS 479
           S+T+T+N +FGA +LSPSTGIVLNNEM DFSIPT     PD  PPAPANFI+P KRPLSS
Sbjct: 389 SMTSTINGYFGAIMLSPSTGIVLNNEMDDFSIPTKSGGDPDVPPPAPANFIRPGKRPLSS 448

Query: 480 MTPIIVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVV 539
           MTP IV KD ++ A +G SGGM II    +V+LN+F L + PL++V   R+YHQLIPN  
Sbjct: 449 MTPTIVLKDGKVKAALGASGGMYIIAGTTQVYLNHFFLNMDPLSSVVAPRIYHQLIPNKA 508

Query: 540 KYENLTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKI 599
            YEN T +  DH E+P+E +  LE +G QV+     G I Q IV+  +     G+ GG  
Sbjct: 509 SYENWTTVYSDHFEIPEEIRLVLEKKG-QVLTPIAGGTISQLIVEQSD-----GKSGG-- 568

Query: 600 SNNQSLFGLLTAVCDPRKNGFPA 620
                    L AV DPRK GFP+
Sbjct: 569 ------ISKLVAVSDPRKGGFPS 576

BLAST of Lcy08g018600 vs. ExPASy Swiss-Prot
Match: Q8VYW6 (Glutathione hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=GGT1 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 6.5e-151
Identity = 280/558 (50.18%), Postives = 371/558 (66.49%), Query Frame = 0

Query: 64  VESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLVRS 123
           +   +G VA DDGRCS IG  +LR GG+A+DA+VA ALCLGVV   SSGIGGG F +V+ 
Sbjct: 29  IVKSRGAVATDDGRCSVIGMRVLREGGNAIDASVAAALCLGVVSPASSGIGGGAFTVVKI 88

Query: 124 GLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLAWK 183
               + IA D RETAPL A+++MY  NV  K  G LSV VPGE+AGL  AW ++G+L WK
Sbjct: 89  A-GGKEIAYDSRETAPLRATENMYGGNVDLKKKGALSVGVPGEVAGLFTAWKQHGKLPWK 148

Query: 184 SLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVELG 243
            L  PA K+A +GF IS +L   + ++   IL D GL  +F  NG+L + G  C+N +L 
Sbjct: 149 RLVTPAEKLA-EGFKISKYLYMQMNATRSDILADKGLSDLFVSNGELKKPGTICHNPKLA 208

Query: 244 KSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYTVH 303
            +L+ + + GP+ FYNG VG  L +D++  GGI+++ DL++Y V++ E ++ + +GY V 
Sbjct: 209 LTLKLIGEYGPKAFYNGTVGVNLARDIKKSGGIITLKDLQSYRVKIKEPLSADILGYRVL 268

Query: 304 GMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFSNIS 363
           GMPPPSSG     +V+NI   Y  P    G +G+HRLIEALKH +A RMNLGDP F++++
Sbjct: 269 GMPPPSSGGAAMMLVLNILSQYGIPSGVSGPLGVHRLIEALKHAFAVRMNLGDPDFTDVT 328

Query: 364 NSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSLTTT 423
             VS+MLSP FAK ++ KI D  TF P YY   W+Q+ DHGTSH  ++D +RNAVS+T+T
Sbjct: 329 KVVSDMLSPKFAKDLKSKINDQKTFDPKYYGGMWNQIDDHGTSHLSIIDRERNAVSMTST 388

Query: 424 VNEHFGAGILSPSTGIVLNNEMGDFSIP--TDISPDKLPPAPANFIQPNKRPLSSMTPII 483
           +N +FGA +LSPSTGIVLNNEM DFSIP  ++ + D  PPAPANFI+P KRPLSSM+P I
Sbjct: 389 INGYFGALMLSPSTGIVLNNEMDDFSIPMKSNGNLDVPPPAPANFIRPGKRPLSSMSPTI 448

Query: 484 VTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYENL 543
           V KD ++ A +G SGG  II    EV+LN+F L + PL++V   R+YHQLIPN   YEN 
Sbjct: 449 VLKDGKVKAAVGASGGANIIAGTTEVYLNHFFLKMDPLSSVLAPRIYHQLIPNRASYENW 508

Query: 544 TFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQS 603
           T +  DH E+P   +  LE +GH V++    G I QFIVQ      ++G   G  S    
Sbjct: 509 TTVFNDHFEIPKATRVVLEKKGH-VLSPIAGGTIAQFIVQ------ESGENSGGRSE--- 568

Query: 604 LFGLLTAVCDPRKNGFPA 620
               L AV DPRK GFP+
Sbjct: 569 ----LVAVSDPRKGGFPS 570

BLAST of Lcy08g018600 vs. ExPASy Swiss-Prot
Match: Q9US04 (Glutathione hydrolase proenzyme 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ggt1 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 7.8e-96
Identity = 207/510 (40.59%), Postives = 291/510 (57.06%), Query Frame = 0

Query: 63  IVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLVR 122
           +VE + GVVA ++  CS+IG  +L+ GG+AVDAA+A+ +C+G V S SSGIGGGGFML+R
Sbjct: 94  VVEGKNGVVATEEETCSQIGVGILKAGGNAVDAAIASGICIGAVNSFSSGIGGGGFMLIR 153

Query: 123 SGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLAW 182
                   +L+FRETAP  AS++M+  N T   VG LSVAVPGEIAG   AW  YG L W
Sbjct: 154 HP-NGTAHSLNFRETAPAGASKNMFHGNSTLSQVGGLSVAVPGEIAGYERAWKMYGSLPW 213

Query: 183 KSLFEPAIKIAKDGFVISPFLGKSL-VSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVE 242
             LFEP I++ +DG  +   L   +           P   ++FAP G  L  G+  Y   
Sbjct: 214 HKLFEPTIRLMRDGMPMPKELASRIRRPEFSYFKTHPDWSKIFAPEGVFLHVGEKFYRPA 273

Query: 243 LGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYT 302
           L  +LE +A  GP+VFY G + E+LVK V+  GGIL+M+D+ N++V V E +        
Sbjct: 274 LASTLEEIAKFGPEVFYTGKIAERLVKFVQQQGGILTMEDMANFSVVVEEPIYGNFYDRE 333

Query: 303 VHGMPPPSSG---TLGFAMVMNIFKSYNDP--DAAKGNIGLHRLIEALKHMYAERMNLGD 362
           V     P SG    LG  ++  +  S           +IG+H LIE +K M A R  L D
Sbjct: 334 VITCGSPCSGEALILGLNVLSKVDLSEGTSILGCEMTDIGVHHLIETMKWMSAGRTVLAD 393

Query: 363 PRFSNISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRN 422
           P F N ++ V  +LS  +A +I+  I +  TF   +Y   +    DHGT+H  V+D D  
Sbjct: 394 PTFYNNTDHVEQLLSLEYADEIRNNISNERTFDFTHYKAEYDFPNDHGTTHLSVIDKDNM 453

Query: 423 AVSLTTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSS 482
           AV LT ++N  FG+ +L P TGI+LN+ M DF+ P  ++   L P+P NFI P KRP SS
Sbjct: 454 AVGLTASINLMFGSQLLEPETGIILNDHMDDFASPGIVNAFGLSPSPYNFIAPGKRPQSS 513

Query: 483 MTPIIVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVV 542
             P I+  +  +  V+GGSGG +I+ AV++  +  +  G   L +V+  R +HQL+PN+V
Sbjct: 514 AVPTILVYNGEVEMVLGGSGGSRIVTAVLDTIIKKYKWGKSLLESVESPRFHHQLMPNIV 573

Query: 543 KYENLTFIDGDHVELPDEKKQFLEDRGHQV 567
                 +ID + VE+  E  + LE  GH V
Sbjct: 574 ------YID-ETVEI--EVLRALEKFGHIV 593

BLAST of Lcy08g018600 vs. ExPASy Swiss-Prot
Match: P0DPU3 (Scoloptoxin SSD14 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.5e-94
Identity = 207/567 (36.51%), Postives = 319/567 (56.26%), Query Frame = 0

Query: 20  VILIPLGLLAITVVGYIIGGSINGSVFHEENKP-FDGVHTDNPEIVESEKGVVAADDGRC 79
           V+ + LG++    + Y+        V  +++ P F G++         ++  VA+D   C
Sbjct: 11  VLFLMLGMIVEEGIAYV-------RVKEKQSSPSFMGIY---------DESAVASDAVPC 70

Query: 80  SEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLVRSGLTLQTIALDFRETA 139
           +E G SMLR+ G AVDAA+AT LC+GV    SSGIGGG FM++    T     +D RE A
Sbjct: 71  AEAGQSMLRQKGSAVDAAIATLLCIGVYNPQSSGIGGGSFMVIYDRSTRTPEVIDAREEA 130

Query: 140 PLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLAWKSLFEPAIKIAKDGFV 199
           P AA+Q M++ +      G LS+AVPGE+ G+  A  R+G+L W++LF+PAI++AK+GF 
Sbjct: 131 PAAATQKMFKGDKKLSSEGGLSIAVPGELRGMALAHERHGKLDWETLFQPAIRLAKEGFP 190

Query: 200 ISPFLGKSLVSSAKLILNDPGLRQVFAP--NGDLLQTGDTCYNVELGKSLEAVADQGPQV 259
           +   L  +L      ILN P L+ VF    +GD+L+  D     +LG++LE +A  G   
Sbjct: 191 VGSELAMALREQRSHILNSPTLKAVFTDPVSGDILKENDIVIRTKLGETLEKIAKNGADE 250

Query: 260 FYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMG-YTVHGMPPPSSGTLGF 319
           FY G V + L+ D+++ GGI++ +DLKNY   +  A      G  T++ +PPPSSG +  
Sbjct: 251 FYEGQVAKDLINDIKSFGGIITAEDLKNYQPVLKNATVAHLSGCLTLYSVPPPSSGYI-L 310

Query: 320 AMVMNI-----FKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFSNISNSVSNML 379
           + ++ +     F   +  D     +  HR +E LK+ YA R  LGD R  N+S+ ++ + 
Sbjct: 311 SFILRVLDKFHFSKASVSDIENATLTYHRFLETLKYAYAYRTKLGDERIENVSSVIAELT 370

Query: 380 SPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSLTTTVNEHFGA 439
           S    +K ++KI D  T+ P +Y  + +   DHGT+H  VV  + +AV +TTT+N  FG+
Sbjct: 371 SEDVIEKTKQKIEDGKTYEPLHYGAQLA-TNDHGTAHISVVSREGDAVVVTTTINYWFGS 430

Query: 440 GILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTP-IIVTKDDRLI 499
           G+ SPSTG++LN+EM DFS P   +   +PP+ ANFI P KRP SS  P I V K   ++
Sbjct: 431 GLRSPSTGVILNDEMDDFSAPDIQNIYGVPPSKANFIVPGKRPQSSTCPSIFVNKGGDVV 490

Query: 500 AVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYENLTFIDGDHV 559
             IG SGG +I  +V    +    LG     A+   R++HQL+P+ ++YE+         
Sbjct: 491 MAIGASGGTRITSSVSLTSMRVLWLGRNIKEAIDEPRLHHQLLPDEIEYES--------- 550

Query: 560 ELPDEKKQFLEDRGHQVVAIDTAGAIV 577
           + P+E  + L+  GH+       G++V
Sbjct: 551 KFPNEILEKLKAIGHKTKPAGAFGSLV 550

BLAST of Lcy08g018600 vs. ExPASy TrEMBL
Match: A0A6J1G2U8 (glutathione hydrolase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450289 PE=4 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 533/620 (85.97%), Postives = 572/620 (92.26%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLLR  ELV K  CR VIL+PLGLLAI VVGYI+GGSING VF+EE+K   G+   N
Sbjct: 33  MESPLLRRRELVNKRRCRGVILVPLGLLAIAVVGYILGGSINGLVFNEESKHSGGIDISN 92

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEIVESEKGVVAADDGRCSEIGASMLR+GGHAVDAAVATALCLGVV SM SGIGGGGFML
Sbjct: 93  PEIVESEKGVVAADDGRCSEIGASMLRQGGHAVDAAVATALCLGVVGSMWSGIGGGGFML 152

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQT A+DFRETAPLAASQ+MYETNVTAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 153 VRSASTLQTTAIDFRETAPLAASQEMYETNVTAKSLGPLSIAVPGEIAGLHEAWLRYGRL 212

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGFVISP++G+SLVSS  +ILNDPGLR+V+APNG ++Q GDTCYNV
Sbjct: 213 AWRSLFEPAIKLAKDGFVISPYVGQSLVSSTDMILNDPGLRRVYAPNGKIVQAGDTCYNV 272

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGPQ FYNG VGEKLVKDVR  GGILSM+DL+NYTVEV EAM TEAMGY
Sbjct: 273 ELGKSLEAVADQGPQAFYNGAVGEKLVKDVREAGGILSMEDLRNYTVEVTEAMTTEAMGY 332

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDPDA KGN+GLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 333 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDATKGNLGLHRLIEALKHMYAERMNLGDPRFS 392

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISNSVSNMLSPSFAKKIQEKIIDN TFPPDYY YRWSQLRD GTSHFC+VDA+RNAVSL
Sbjct: 393 NISNSVSNMLSPSFAKKIQEKIIDNATFPPDYYQYRWSQLRDSGTSHFCIVDAERNAVSL 452

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVN HFGAG+LSPSTGIVLNNEMGDFS+PTDISPDKLPPAPANFIQPNKRPLSSM P+
Sbjct: 453 TTTVNAHFGAGLLSPSTGIVLNNEMGDFSVPTDISPDKLPPAPANFIQPNKRPLSSMIPL 512

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKIIPAVI+VFLNYFSLG QP  AV+RSRVYHQLIPN+VKYEN
Sbjct: 513 IVTKDDRLIAVLGGSGGMKIIPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNIVKYEN 572

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTFIDGDHVEL DEK  FLE+RGH+VVAID  GAIVQFIVQNFE+AID GRKG KISN+Q
Sbjct: 573 LTFIDGDHVELSDEKTAFLEERGHKVVAIDAPGAIVQFIVQNFEDAIDKGRKGRKISNDQ 632

Query: 601 SLFGLLTAVCDPRKNGFPAV 621
           + FG+LTAVCDPRKNG PA+
Sbjct: 633 THFGVLTAVCDPRKNGNPAI 652

BLAST of Lcy08g018600 vs. ExPASy TrEMBL
Match: A0A6J1DN40 (gamma-glutamyltranspeptidase 3 OS=Momordica charantia OX=3673 GN=LOC111021933 PE=4 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.3e-310
Identity = 522/621 (84.06%), Postives = 572/621 (92.11%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLL SH    KW CRR I +PLGLLA  VVG+I+GGSIN SVF EENK FDG+H +N
Sbjct: 1   MESPLLGSHGSEDKWRCRRAIFVPLGLLATVVVGHILGGSINCSVFREENKHFDGIHLEN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEI++SE+GVVAADDGRCSEIGAS+LR+GGHAVDAAVATALCLGVVCS+SSGIGGGGFML
Sbjct: 61  PEIIQSEEGVVAADDGRCSEIGASILRQGGHAVDAAVATALCLGVVCSLSSGIGGGGFML 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  T QTIA DFRETAPLAASQ MYE NVTAK  GPLS+ VPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSSPTWQTIAFDFRETAPLAASQGMYEINVTAKRFGPLSIGVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
            W+SL EPAIK+AKDGF IS FLG+ LVS A +ILNDPGLRQV+APNGDLLQ GDTCYN+
Sbjct: 181 PWRSLIEPAIKLAKDGFAISHFLGEGLVSCANIILNDPGLRQVYAPNGDLLQVGDTCYNI 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGP+ FYNGI+GEKLVKDVRA+GGILSM+DL+NYTV++ EA+ TEA+GY
Sbjct: 241 ELGKSLEAVADQGPEAFYNGIIGEKLVKDVRAIGGILSMEDLRNYTVDITEAITTEAIGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDP A KG++GLHRLIEALKHM+AERMNLGDPRFS
Sbjct: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPAATKGSLGLHRLIEALKHMFAERMNLGDPRFS 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NI++SVS ML+PSFAKKIQEKI+DNTTFPPDYYLYRWSQLRDHGTSHFC+VDA+RNAVSL
Sbjct: 361 NINDSVSYMLNPSFAKKIQEKIVDNTTFPPDYYLYRWSQLRDHGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVNEHFGAGILSPSTGIVLNNEMGDFSI TD+SPDKLPPAPANFIQPNKRPLSSMTP+
Sbjct: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIATDVSPDKLPPAPANFIQPNKRPLSSMTPL 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDD+LIAVIGGSGGMKIIPAVI+VFLNYFSLGLQPLAAVQ SRVYHQLIPNVV+YEN
Sbjct: 481 IVTKDDQLIAVIGGSGGMKIIPAVIQVFLNYFSLGLQPLAAVQSSRVYHQLIPNVVRYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTF+DGDHVEL +EKKQFLE+RGH+VV  + AGA+VQ IVQNF++ ID GRKGGKI NNQ
Sbjct: 541 LTFMDGDHVELTEEKKQFLEERGHEVVVSNIAGAMVQLIVQNFKDTIDAGRKGGKIPNNQ 600

Query: 601 SLFGLLTAVCDPRKNGFPAVV 622
           +LFGLLTAV DPRKNGFPA V
Sbjct: 601 TLFGLLTAVSDPRKNGFPAAV 621

BLAST of Lcy08g018600 vs. ExPASy TrEMBL
Match: A0A6J1KAW6 (glutathione hydrolase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493812 PE=4 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.2e-309
Identity = 531/620 (85.65%), Postives = 569/620 (91.77%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLL   ELV    CR VIL+PLGLLAITVVGYI+GGSING VF+EE+K   G+ T N
Sbjct: 1   MESPLLLCRELVNMRRCRGVILVPLGLLAITVVGYILGGSINGLVFNEESKNSGGIDTSN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEIVESEKGVVAADDGRCSEIGASMLR GGHAVDAAVATALCLGVV SM SGIGGGGFML
Sbjct: 61  PEIVESEKGVVAADDGRCSEIGASMLRPGGHAVDAAVATALCLGVVGSMWSGIGGGGFML 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQT A+DFRETAPLAASQDMYETNVT K +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQTTAIDFRETAPLAASQDMYETNVTTKSLGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGFVISP++G+SLVSS  +ILNDPGLR+V+APNG ++Q GDTCYNV
Sbjct: 181 AWRSLFEPAIKLAKDGFVISPYVGQSLVSSTNMILNDPGLRRVYAPNGKIVQAGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGPQ FY G VGEKLVKDVR  GGILSM+DL+NYTVEV EAM TEAMGY
Sbjct: 241 ELGKSLEAVADQGPQAFYYGAVGEKLVKDVREAGGILSMEDLRNYTVEVTEAMTTEAMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDPDA KGN+GLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDATKGNLGLHRLIEALKHMYAERMNLGDPRFS 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISNSVSNMLSPSFAKKIQEKIIDN TFPPDYY YRWSQLRD GTSHFC+VDA+RNAVSL
Sbjct: 361 NISNSVSNMLSPSFAKKIQEKIIDNATFPPDYYQYRWSQLRDSGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVNEHFGAG+LSPSTGIVLNNEMGDFS+PTDISPDKLPPAPANFIQPNKRPLSSM P+
Sbjct: 421 TTTVNEHFGAGLLSPSTGIVLNNEMGDFSVPTDISPDKLPPAPANFIQPNKRPLSSMIPL 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKIIPAVI+VFLNYFSLG QP  AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVLGGSGGMKIIPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTFIDGDHVEL +EK  FLE+RGH+VVAID  GAIVQFIVQNFE++ID GRKG KISN++
Sbjct: 541 LTFIDGDHVELSNEKTAFLEERGHKVVAIDAPGAIVQFIVQNFEDSIDKGRKGRKISNDR 600

Query: 601 SLFGLLTAVCDPRKNGFPAV 621
           +  G+LTAVCDPRKNG PAV
Sbjct: 601 THLGVLTAVCDPRKNGNPAV 620

BLAST of Lcy08g018600 vs. ExPASy TrEMBL
Match: A0A5D3CHL7 (Gamma-glutamyltranspeptidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00750 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 3.0e-308
Identity = 524/621 (84.38%), Postives = 573/621 (92.27%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           M+SPLLR  E   K  CR +I +PLG+LAITVVG+I+GGSIN SVF+ ENK FDG+ T N
Sbjct: 1   MDSPLLRRGESADKRCCRGLIFLPLGILAITVVGHILGGSINDSVFNGENKYFDGIGTYN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
            EIVESEKGVVAADDGRCSEIGAS+LR+GGHAVDAAVATALCLGVVCSM SGIGGGGFM+
Sbjct: 61  SEIVESEKGVVAADDGRCSEIGASVLRKGGHAVDAAVATALCLGVVCSMWSGIGGGGFMV 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQ IA+DFRETAPLAASQDMYE N+TAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQAIAIDFRETAPLAASQDMYEANLTAKDIGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AWKSLFEPAIK+AKDGFVISP+LG+SLVSS ++IL+DPGLRQVFAPNG++LQ GDTCYNV
Sbjct: 181 AWKSLFEPAIKLAKDGFVISPYLGQSLVSSTEMILHDPGLRQVFAPNGNILQVGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVA+QGPQ FYNG+VGEKLVKDVRAVGGIL+M+DL+NYTVE+ EAM  EAMGY
Sbjct: 241 ELGKSLEAVANQGPQAFYNGVVGEKLVKDVRAVGGILTMEDLRNYTVEITEAMTIEAMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLG AMVMNIFKSYNDPDA KGN+G+HRLIEALKHMYAERMNLGDPRF+
Sbjct: 301 TVHGMPPPSSGTLGLAMVMNIFKSYNDPDATKGNLGVHRLIEALKHMYAERMNLGDPRFA 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISN+VSNMLS SFAKKIQEKI+DNTTFP DYY YRWSQLRDHGTSHFC+VDA+RNAVSL
Sbjct: 361 NISNAVSNMLSLSFAKKIQEKIVDNTTFPSDYYQYRWSQLRDHGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVN H GAGILSPSTGIVLNNEMGDFS PT+ISPDKLPPAP NF+QPNKRPLSSMTPI
Sbjct: 421 TTTVNGHLGAGILSPSTGIVLNNEMGDFSTPTNISPDKLPPAPTNFVQPNKRPLSSMTPI 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKI+PAVI+VFLNYFSLG QP +AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVMGGSGGMKILPAVIQVFLNYFSLGFQPFSAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LT I+GDHVE   ++KQFLEDRGHQVVA+D AGAIVQ +VQNFE+AID GRKGGKISNNQ
Sbjct: 541 LTCINGDHVEFSAKEKQFLEDRGHQVVAMDAAGAIVQLVVQNFEDAIDIGRKGGKISNNQ 600

Query: 601 SLFGLLTAVCDPRKNGFPAVV 622
             FGLLTAVCDPRKNG PAVV
Sbjct: 601 QDFGLLTAVCDPRKNGNPAVV 621

BLAST of Lcy08g018600 vs. ExPASy TrEMBL
Match: A0A1S3C9J3 (gamma-glutamyltranspeptidase 3 OS=Cucumis melo OX=3656 GN=LOC103498319 PE=4 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 2.5e-307
Identity = 523/621 (84.22%), Postives = 572/621 (92.11%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           M+SPLLR  E   K  CR +I +PLG+LAITVVG+I+GGSIN SVF+ ENK  DG+ T N
Sbjct: 1   MDSPLLRRGESADKRCCRGLIFLPLGILAITVVGHILGGSINDSVFNGENKYSDGIGTYN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
            EIVESEKGVVAADDGRCSEIGAS+LR+GGHAVDAAVATALCLGVVCSM SGIGGGGFM+
Sbjct: 61  SEIVESEKGVVAADDGRCSEIGASVLRKGGHAVDAAVATALCLGVVCSMWSGIGGGGFMV 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQ IA+DFRETAPLAASQDMYE N+TAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQAIAIDFRETAPLAASQDMYEANLTAKDIGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AWKSLFEPAIK+AKDGFVISP+LG+SLVSS ++IL+DPGLRQVFAPNG++LQ GDTCYNV
Sbjct: 181 AWKSLFEPAIKLAKDGFVISPYLGQSLVSSTEMILHDPGLRQVFAPNGNILQVGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVA+QGPQ FYNG+VGEKLVKDVRAVGGIL+M+DL+NYTVE+ EAM  EAMGY
Sbjct: 241 ELGKSLEAVANQGPQAFYNGVVGEKLVKDVRAVGGILTMEDLRNYTVEITEAMTIEAMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLG AMVMNIFKSYNDPDA KGN+G+HRLIEALKHMYAERMNLGDPRF+
Sbjct: 301 TVHGMPPPSSGTLGLAMVMNIFKSYNDPDATKGNLGVHRLIEALKHMYAERMNLGDPRFA 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISN+VSNMLS SFAKKIQEKI+DNTTFP DYY YRWSQLRDHGTSHFC+VDA+RNAVSL
Sbjct: 361 NISNAVSNMLSLSFAKKIQEKIVDNTTFPSDYYQYRWSQLRDHGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVN H GAGILSPSTGIVLNNEMGDFS PT+ISPDKLPPAP NF+QPNKRPLSSMTPI
Sbjct: 421 TTTVNGHLGAGILSPSTGIVLNNEMGDFSTPTNISPDKLPPAPTNFVQPNKRPLSSMTPI 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKI+PAVI+VFLNYFSLG QP +AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVMGGSGGMKILPAVIQVFLNYFSLGFQPFSAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LT I+GDHVE   ++KQFLEDRGHQVVA+D AGAIVQ +VQNFE+AID GRKGGKISNNQ
Sbjct: 541 LTCINGDHVEFSAKEKQFLEDRGHQVVAMDAAGAIVQLVVQNFEDAIDIGRKGGKISNNQ 600

Query: 601 SLFGLLTAVCDPRKNGFPAVV 622
             FGLLTAVCDPRKNG PAVV
Sbjct: 601 QDFGLLTAVCDPRKNGNPAVV 621

BLAST of Lcy08g018600 vs. NCBI nr
Match: XP_038890419.1 (glutathione hydrolase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 538/621 (86.63%), Postives = 582/621 (93.72%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLLR  E V +W CR VI IPLG+LA+TVVG+I+GGSINGSVF+EENK  DG+H +N
Sbjct: 1   MESPLLRHRESVDRWCCRGVIFIPLGILALTVVGHILGGSINGSVFNEENKHSDGIHINN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
            E VESEKGVVAADDGRCSEIGASMLR+GGHAVDAAVATALCLGVVCSM SGIGGGGFM+
Sbjct: 61  SETVESEKGVVAADDGRCSEIGASMLRQGGHAVDAAVATALCLGVVCSMWSGIGGGGFMV 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQTIA+DFRETAPLAASQDMYE+NVTAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQTIAIDFRETAPLAASQDMYESNVTAKDIGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGF+ISP+LGKSLVSSAK+IL+DPGLRQVFAPNGD+LQ GDTCYNV
Sbjct: 181 AWRSLFEPAIKLAKDGFMISPYLGKSLVSSAKMILHDPGLRQVFAPNGDILQVGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVA+QGPQ FYNG+VGEKLVKDVRAVGGILSM+DL+NYTVE+ EAM TE MGY
Sbjct: 241 ELGKSLEAVANQGPQAFYNGVVGEKLVKDVRAVGGILSMEDLRNYTVEITEAMTTETMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGT+G AMVMNIFKSYNDPDA KGNIGLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 301 TVHGMPPPSSGTVGLAMVMNIFKSYNDPDATKGNIGLHRLIEALKHMYAERMNLGDPRFS 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NI+N VSNMLSPSFAKK+QEKI+DNTTFPPDYYLYRWSQLRDHGTSHFC+VDA+RNAVSL
Sbjct: 361 NINNVVSNMLSPSFAKKVQEKIVDNTTFPPDYYLYRWSQLRDHGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVN   GAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI
Sbjct: 421 TTTVNGRLGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAVIGGSGGMKI+PAVI+VFLNYFSLG QP  AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVIGGSGGMKILPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LT I+GDHV    ++KQFLEDRGHQVVA+DTAGAIVQF+VQNF++ +DTGRKGGKISNN+
Sbjct: 541 LTCINGDHVVFSAKEKQFLEDRGHQVVAMDTAGAIVQFVVQNFKDVVDTGRKGGKISNNR 600

Query: 601 SLFGLLTAVCDPRKNGFPAVV 622
             FGLLTAVCDPRKNG PAVV
Sbjct: 601 KHFGLLTAVCDPRKNGNPAVV 621

BLAST of Lcy08g018600 vs. NCBI nr
Match: XP_023521514.1 (glutathione hydrolase 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 537/620 (86.61%), Postives = 574/620 (92.58%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLLR  ELV K  CR VIL+PLGLLAI VVGYI+GGSING VF+EE+K   G+   N
Sbjct: 1   MESPLLRRRELVNKRRCRGVILVPLGLLAIAVVGYILGGSINGLVFNEESKHSGGIDISN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEIVESEKGVVAADDGRCSEIGASMLR+GGHAVDAAVATALCLGVV SM SGIGGGGFML
Sbjct: 61  PEIVESEKGVVAADDGRCSEIGASMLRQGGHAVDAAVATALCLGVVGSMWSGIGGGGFML 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQT A+DFRETAPLAASQDMYETNVTAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQTTAIDFRETAPLAASQDMYETNVTAKSLGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGFVISP++G+SLVSS  +ILNDPGLR+V+APNG ++Q GDTCYNV
Sbjct: 181 AWRSLFEPAIKLAKDGFVISPYVGQSLVSSTNMILNDPGLRRVYAPNGKIVQAGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGPQ FYNG VGEKLVKDVR  GGILSM+DL+NYTVEV EAM TEAMGY
Sbjct: 241 ELGKSLEAVADQGPQAFYNGAVGEKLVKDVREAGGILSMEDLRNYTVEVTEAMTTEAMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDPDA KGN+GLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDATKGNLGLHRLIEALKHMYAERMNLGDPRFS 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYY YRWSQLRD GTSHFC+VDA+RNAVSL
Sbjct: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYQYRWSQLRDSGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVNEHFGAG+LSPSTGIVLNNEMGDFS+PTDISPDKLPPAPANFIQPNKRPLSSM P+
Sbjct: 421 TTTVNEHFGAGLLSPSTGIVLNNEMGDFSVPTDISPDKLPPAPANFIQPNKRPLSSMIPL 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKIIPAVI+VFLNYFSLG QP  AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVLGGSGGMKIIPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTFIDGDHVEL DEK  FLE+RGH+VVAID  GAIVQFIVQNFE+AID GRKG KISN++
Sbjct: 541 LTFIDGDHVELSDEKTAFLEERGHKVVAIDAPGAIVQFIVQNFEDAIDKGRKGRKISNDR 600

Query: 601 SLFGLLTAVCDPRKNGFPAV 621
           + FG+LTAVCDPRKNG PAV
Sbjct: 601 THFGVLTAVCDPRKNGNPAV 620

BLAST of Lcy08g018600 vs. NCBI nr
Match: XP_023547246.1 (glutathione hydrolase 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 536/620 (86.45%), Postives = 574/620 (92.58%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLLR  ELV K  CR VIL+PLGLLAI VVGYI+GGSING VF+EE+K   G+   N
Sbjct: 1   MESPLLRRRELVNKRRCRGVILVPLGLLAIAVVGYILGGSINGLVFNEESKHSGGIDISN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEIVESEKGVVAADDGRCSEIGASMLR+GGHAVDAAVATALCLGVV SM SGIGGGGFML
Sbjct: 61  PEIVESEKGVVAADDGRCSEIGASMLRQGGHAVDAAVATALCLGVVGSMWSGIGGGGFML 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQT A+DFRETAPLAASQDMYETNVTAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQTTAIDFRETAPLAASQDMYETNVTAKSLGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGFVISP++G+SLVSS  +ILNDPGLR+V+APNG ++Q GDTCYNV
Sbjct: 181 AWRSLFEPAIKLAKDGFVISPYVGQSLVSSTNMILNDPGLRRVYAPNGKIVQAGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGPQ FYNG VGEKLVKDVR  GGILSM+DL+NYTVEV EAM TEAMGY
Sbjct: 241 ELGKSLEAVADQGPQAFYNGAVGEKLVKDVREAGGILSMEDLRNYTVEVTEAMTTEAMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDPDA KGN+GLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDATKGNLGLHRLIEALKHMYAERMNLGDPRFS 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYY YRWSQLRD GTSHFC+VDA+RNAVSL
Sbjct: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYQYRWSQLRDSGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVNEHFGAG+LSPSTGIVLNNEMGDFS+PTDISPDKLPPAPANFIQPNKRPLSSM P+
Sbjct: 421 TTTVNEHFGAGLLSPSTGIVLNNEMGDFSVPTDISPDKLPPAPANFIQPNKRPLSSMIPL 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKIIPAVI+VFLNYFSLG QP  AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVLGGSGGMKIIPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTFIDGDHVEL DEK  FLE+RGH+VVAID  GAIVQFIVQNFE+AID GRKG KISN++
Sbjct: 541 LTFIDGDHVELSDEKTAFLEERGHKVVAIDAPGAIVQFIVQNFEDAIDKGRKGRKISNDR 600

Query: 601 SLFGLLTAVCDPRKNGFPAV 621
           + FG+LTAVCDPRKNG PA+
Sbjct: 601 THFGVLTAVCDPRKNGNPAI 620

BLAST of Lcy08g018600 vs. NCBI nr
Match: KAG6599495.1 (Glutathione hydrolase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 535/620 (86.29%), Postives = 572/620 (92.26%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLLR  ELV K  CR VIL+PLGLLAI VVGYI+GGSING VF+EE+K   G+   N
Sbjct: 1   MESPLLRRRELVNKRRCRGVILVPLGLLAIAVVGYILGGSINGLVFNEESKHSGGIDISN 60

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEIVESEKGVVAADDGRCSEIGASMLR+GGHAVDAAVATALCLGVV SM SGIGGGGFML
Sbjct: 61  PEIVESEKGVVAADDGRCSEIGASMLRQGGHAVDAAVATALCLGVVGSMWSGIGGGGFML 120

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQT A+DFRETAPLAASQDMYETNVTAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 121 VRSASTLQTTAIDFRETAPLAASQDMYETNVTAKSLGPLSIAVPGEIAGLHEAWLRYGRL 180

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGFVISP++G+SLVSS  +ILNDPGLR+V+APNG ++Q GDTCYNV
Sbjct: 181 AWRSLFEPAIKLAKDGFVISPYVGQSLVSSTDMILNDPGLRRVYAPNGKIVQAGDTCYNV 240

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGPQ FYNG VGEKLVKDVR  GGILSM+DL+NYTVEV EAM TEAMGY
Sbjct: 241 ELGKSLEAVADQGPQAFYNGAVGEKLVKDVREAGGILSMEDLRNYTVEVTEAMTTEAMGY 300

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDPDA KGN+GLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDATKGNLGLHRLIEALKHMYAERMNLGDPRFS 360

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISNSVSNMLSPSFAKKIQEKIIDN TFPPDYY YRWSQLRD GTSHFC+VDA+RNAVSL
Sbjct: 361 NISNSVSNMLSPSFAKKIQEKIIDNATFPPDYYQYRWSQLRDSGTSHFCIVDAERNAVSL 420

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVN HFGAG+LSPSTGIVLNNEMGDFS+PTDISPDKLPPAPANFIQPNKRPLSSM P+
Sbjct: 421 TTTVNAHFGAGLLSPSTGIVLNNEMGDFSVPTDISPDKLPPAPANFIQPNKRPLSSMIPL 480

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKIIPAVI+VFLNYFSLG QP  AV+RSRVYHQLIPNVVKYEN
Sbjct: 481 IVTKDDRLIAVLGGSGGMKIIPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNVVKYEN 540

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTFIDGDHVEL DEK  FLE+RGH+VVAID  GAIVQFIVQNFE+AID GRKG KISN+Q
Sbjct: 541 LTFIDGDHVELSDEKTAFLEERGHKVVAIDAPGAIVQFIVQNFEDAIDKGRKGRKISNDQ 600

Query: 601 SLFGLLTAVCDPRKNGFPAV 621
           + FG+LTAVCDPRKNG PA+
Sbjct: 601 THFGVLTAVCDPRKNGNPAI 620

BLAST of Lcy08g018600 vs. NCBI nr
Match: XP_022946100.1 (glutathione hydrolase 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 533/620 (85.97%), Postives = 572/620 (92.26%), Query Frame = 0

Query: 1   MESPLLRSHELVGKWSCRRVILIPLGLLAITVVGYIIGGSINGSVFHEENKPFDGVHTDN 60
           MESPLLR  ELV K  CR VIL+PLGLLAI VVGYI+GGSING VF+EE+K   G+   N
Sbjct: 33  MESPLLRRRELVNKRRCRGVILVPLGLLAIAVVGYILGGSINGLVFNEESKHSGGIDISN 92

Query: 61  PEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFML 120
           PEIVESEKGVVAADDGRCSEIGASMLR+GGHAVDAAVATALCLGVV SM SGIGGGGFML
Sbjct: 93  PEIVESEKGVVAADDGRCSEIGASMLRQGGHAVDAAVATALCLGVVGSMWSGIGGGGFML 152

Query: 121 VRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRL 180
           VRS  TLQT A+DFRETAPLAASQ+MYETNVTAK +GPLS+AVPGEIAGLHEAWLRYGRL
Sbjct: 153 VRSASTLQTTAIDFRETAPLAASQEMYETNVTAKSLGPLSIAVPGEIAGLHEAWLRYGRL 212

Query: 181 AWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNV 240
           AW+SLFEPAIK+AKDGFVISP++G+SLVSS  +ILNDPGLR+V+APNG ++Q GDTCYNV
Sbjct: 213 AWRSLFEPAIKLAKDGFVISPYVGQSLVSSTDMILNDPGLRRVYAPNGKIVQAGDTCYNV 272

Query: 241 ELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGY 300
           ELGKSLEAVADQGPQ FYNG VGEKLVKDVR  GGILSM+DL+NYTVEV EAM TEAMGY
Sbjct: 273 ELGKSLEAVADQGPQAFYNGAVGEKLVKDVREAGGILSMEDLRNYTVEVTEAMTTEAMGY 332

Query: 301 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFS 360
           TVHGMPPPSSGTLGFAMVMNIFKSYNDPDA KGN+GLHRLIEALKHMYAERMNLGDPRFS
Sbjct: 333 TVHGMPPPSSGTLGFAMVMNIFKSYNDPDATKGNLGLHRLIEALKHMYAERMNLGDPRFS 392

Query: 361 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 420
           NISNSVSNMLSPSFAKKIQEKIIDN TFPPDYY YRWSQLRD GTSHFC+VDA+RNAVSL
Sbjct: 393 NISNSVSNMLSPSFAKKIQEKIIDNATFPPDYYQYRWSQLRDSGTSHFCIVDAERNAVSL 452

Query: 421 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 480
           TTTVN HFGAG+LSPSTGIVLNNEMGDFS+PTDISPDKLPPAPANFIQPNKRPLSSM P+
Sbjct: 453 TTTVNAHFGAGLLSPSTGIVLNNEMGDFSVPTDISPDKLPPAPANFIQPNKRPLSSMIPL 512

Query: 481 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 540
           IVTKDDRLIAV+GGSGGMKIIPAVI+VFLNYFSLG QP  AV+RSRVYHQLIPN+VKYEN
Sbjct: 513 IVTKDDRLIAVLGGSGGMKIIPAVIQVFLNYFSLGFQPFPAVERSRVYHQLIPNIVKYEN 572

Query: 541 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQ 600
           LTFIDGDHVEL DEK  FLE+RGH+VVAID  GAIVQFIVQNFE+AID GRKG KISN+Q
Sbjct: 573 LTFIDGDHVELSDEKTAFLEERGHKVVAIDAPGAIVQFIVQNFEDAIDKGRKGRKISNDQ 632

Query: 601 SLFGLLTAVCDPRKNGFPAV 621
           + FG+LTAVCDPRKNG PA+
Sbjct: 633 THFGVLTAVCDPRKNGNPAI 652

BLAST of Lcy08g018600 vs. TAIR 10
Match: AT4G29210.1 (gamma-glutamyl transpeptidase 4 )

HSP 1 Score: 741.5 bits (1913), Expect = 5.5e-214
Identity = 360/567 (63.49%), Postives = 455/567 (80.25%), Query Frame = 0

Query: 62  EIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLV 121
           ++VESE GVVAADD RCSEIGAS+LR GGHAVDAAVA  LC+GVV  MSSGIGGG F++V
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRSGGHAVDAAVAITLCVGVVNPMSSGIGGGSFLIV 133

Query: 122 RSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLA 181
            S    +  A D RETAPLAAS+DMY+ + +AK +G LS+ VPGEIAGL+EAW RYGRL 
Sbjct: 134 SSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRLP 193

Query: 182 WKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVE 241
           WK LFEPAIK+A+DGFV+ P+LGK++ +   +IL DPG+R VF+ NG +L+TG+TCYN E
Sbjct: 194 WKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSRNGQVLKTGETCYNPE 253

Query: 242 LGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYT 301
           L +SLE +++QGP  FYNG VGEKLVKDV+  GGI++MDDL++Y V V +AM+ + MGYT
Sbjct: 254 LAQSLETISEQGPGAFYNGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAMSVDVMGYT 313

Query: 302 VHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKG-NIGLHRLIEALKHMYAERMNLGDPRFS 361
           VHGMPPPS GT+GF+MVMNI  SY++   A G  +GLHRLIEA+KHM+A RM+LGDP F 
Sbjct: 314 VHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMDLGDPEFV 373

Query: 362 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 421
           N++NS++ MLS + A++IQ++I DNTTFPP+YY+ RWSQLRD GTSHFCVVDADRN+VS+
Sbjct: 374 NVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDADRNSVSM 433

Query: 422 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 481
           T+TVN  FGAG+LSPSTGIVLNNEM DFS PT+I+PD LPPAP NFI+PNKRPLSSMTP+
Sbjct: 434 TSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRPLSSMTPL 493

Query: 482 IVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYEN 541
           ++TKD   +A +GG+GGM IIPAV++VFLN F L ++P  AV+ +R+YH+LIPNVV YEN
Sbjct: 494 VITKDGEFVAALGGAGGMHIIPAVLQVFLNCFVLNMKPKEAVESARIYHRLIPNVVSYEN 553

Query: 542 LTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEE------AIDTGRKGG 601
            T I+GDH+ + ++ K FL +RGH++  + + GAIVQ IVQ+F+E       I+ GRK G
Sbjct: 554 FTTINGDHIGVSEDTKMFLAERGHELKEL-SGGAIVQLIVQSFKEEKEEEMIIEIGRKIG 613

Query: 602 KISNNQSLFGLLTAVCDPRKNGFPAVV 622
           K   ++ L GLLTAV DPRK+G PA V
Sbjct: 614 K--KSKPLKGLLTAVSDPRKDGKPAAV 637

BLAST of Lcy08g018600 vs. TAIR 10
Match: AT4G29210.2 (gamma-glutamyl transpeptidase 4 )

HSP 1 Score: 601.3 bits (1549), Expect = 8.9e-172
Identity = 285/424 (67.22%), Postives = 353/424 (83.25%), Query Frame = 0

Query: 62  EIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLV 121
           ++VESE GVVAADD RCSEIGAS+LR GGHAVDAAVA  LC+GVV  MSSGIGGG F++V
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRSGGHAVDAAVAITLCVGVVNPMSSGIGGGSFLIV 133

Query: 122 RSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLA 181
            S    +  A D RETAPLAAS+DMY+ + +AK +G LS+ VPGEIAGL+EAW RYGRL 
Sbjct: 134 SSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRLP 193

Query: 182 WKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVE 241
           WK LFEPAIK+A+DGFV+ P+LGK++ +   +IL DPG+R VF+ NG +L+TG+TCYN E
Sbjct: 194 WKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSRNGQVLKTGETCYNPE 253

Query: 242 LGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYT 301
           L +SLE +++QGP  FYNG VGEKLVKDV+  GGI++MDDL++Y V V +AM+ + MGYT
Sbjct: 254 LAQSLETISEQGPGAFYNGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAMSVDVMGYT 313

Query: 302 VHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKG-NIGLHRLIEALKHMYAERMNLGDPRFS 361
           VHGMPPPS GT+GF+MVMNI  SY++   A G  +GLHRLIEA+KHM+A RM+LGDP F 
Sbjct: 314 VHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMDLGDPEFV 373

Query: 362 NISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSL 421
           N++NS++ MLS + A++IQ++I DNTTFPP+YY+ RWSQLRD GTSHFCVVDADRN+VS+
Sbjct: 374 NVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDADRNSVSM 433

Query: 422 TTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDISPDKLPPAPANFIQPNKRPLSSMTPI 481
           T+TVN  FGAG+LSPSTGIVLNNEM DFS PT+I+PD LPPAP NFI+PNKRPLSSMTP+
Sbjct: 434 TSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRPLSSMTPL 493

Query: 482 IVTK 485
           ++TK
Sbjct: 494 VITK 497

BLAST of Lcy08g018600 vs. TAIR 10
Match: AT4G39650.1 (gamma-glutamyl transpeptidase 2 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.6e-157
Identity = 284/563 (50.44%), Postives = 379/563 (67.32%), Query Frame = 0

Query: 60  NPEIVESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFM 119
           N + + +  G VA DDGRCS IG ++LR+GG+A+DA+VA ALCLGVV   SSGIGGG F 
Sbjct: 29  NLQSIVAYHGAVATDDGRCSAIGTNVLRQGGNAIDASVAAALCLGVVSPASSGIGGGAFT 88

Query: 120 LVRSGLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGR 179
           +++     + +A D RETAPL+A++DMY  N   K  G LSV VPGE+AGL+ AW ++G+
Sbjct: 89  MIKLANGTE-VAYDSRETAPLSATEDMYGDNPERKKKGSLSVGVPGEVAGLYTAWTQHGK 148

Query: 180 LAWKSLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYN 239
           L WK L EPA K+A +GF IS +L   + ++   IL D GL ++F  NG+L + G  C N
Sbjct: 149 LPWKQLVEPAEKLAAEGFKISKYLYMQMNATRSDILADKGLSELFVSNGELKKPGAICRN 208

Query: 240 VELGKSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMG 299
            +L  +L  +A+ GP+ FYNG VG  LV D++  GGI+++ DL+NY V+V E ++TE +G
Sbjct: 209 PKLADTLSQIAEYGPKAFYNGTVGFNLVSDIQKAGGIITLKDLQNYNVKVKEPLSTEILG 268

Query: 300 YTVHGMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRF 359
           Y + GMPPPSSG     +++NI   Y  P    G +G+HRL+EALKH +A RMNLGDP F
Sbjct: 269 YRLLGMPPPSSGGPAMMLILNILAQYGIPSGVSGPLGVHRLVEALKHAFAVRMNLGDPDF 328

Query: 360 -SNISNSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAV 419
              ++N V++MLSP FA+ ++ KI D  TF P YY  +W Q++DHGTSH  ++D++RNAV
Sbjct: 329 VPEVTNVVADMLSPKFAQDLKSKINDEKTFDPKYYGGKWGQIKDHGTSHLSIIDSERNAV 388

Query: 420 SLTTTVNEHFGAGILSPSTGIVLNNEMGDFSIPTDI--SPDKLPPAPANFIQPNKRPLSS 479
           S+T+T+N +FGA +LSPSTGIVLNNEM DFSIPT     PD  PPAPANFI+P KRPLSS
Sbjct: 389 SMTSTINGYFGAIMLSPSTGIVLNNEMDDFSIPTKSGGDPDVPPPAPANFIRPGKRPLSS 448

Query: 480 MTPIIVTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVV 539
           MTP IV KD ++ A +G SGGM II    +V+LN+F L + PL++V   R+YHQLIPN  
Sbjct: 449 MTPTIVLKDGKVKAALGASGGMYIIAGTTQVYLNHFFLNMDPLSSVVAPRIYHQLIPNKA 508

Query: 540 KYENLTFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKI 599
            YEN T +  DH E+P+E +  LE +G QV+     G I Q IV+  +     G+ GG  
Sbjct: 509 SYENWTTVYSDHFEIPEEIRLVLEKKG-QVLTPIAGGTISQLIVEQSD-----GKSGG-- 568

Query: 600 SNNQSLFGLLTAVCDPRKNGFPA 620
                    L AV DPRK GFP+
Sbjct: 569 ------ISKLVAVSDPRKGGFPS 576

BLAST of Lcy08g018600 vs. TAIR 10
Match: AT4G39640.1 (gamma-glutamyl transpeptidase 1 )

HSP 1 Score: 535.8 bits (1379), Expect = 4.6e-152
Identity = 280/558 (50.18%), Postives = 371/558 (66.49%), Query Frame = 0

Query: 64  VESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLVRS 123
           +   +G VA DDGRCS IG  +LR GG+A+DA+VA ALCLGVV   SSGIGGG F +V+ 
Sbjct: 29  IVKSRGAVATDDGRCSVIGMRVLREGGNAIDASVAAALCLGVVSPASSGIGGGAFTVVKI 88

Query: 124 GLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLAWK 183
               + IA D RETAPL A+++MY  NV  K  G LSV VPGE+AGL  AW ++G+L WK
Sbjct: 89  A-GGKEIAYDSRETAPLRATENMYGGNVDLKKKGALSVGVPGEVAGLFTAWKQHGKLPWK 148

Query: 184 SLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVELG 243
            L  PA K+A +GF IS +L   + ++   IL D GL  +F  NG+L + G  C+N +L 
Sbjct: 149 RLVTPAEKLA-EGFKISKYLYMQMNATRSDILADKGLSDLFVSNGELKKPGTICHNPKLA 208

Query: 244 KSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYTVH 303
            +L+ + + GP+ FYNG VG  L +D++  GGI+++ DL++Y V++ E ++ + +GY V 
Sbjct: 209 LTLKLIGEYGPKAFYNGTVGVNLARDIKKSGGIITLKDLQSYRVKIKEPLSADILGYRVL 268

Query: 304 GMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFSNIS 363
           GMPPPSSG     +V+NI   Y  P    G +G+HRLIEALKH +A RMNLGDP F++++
Sbjct: 269 GMPPPSSGGAAMMLVLNILSQYGIPSGVSGPLGVHRLIEALKHAFAVRMNLGDPDFTDVT 328

Query: 364 NSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSLTTT 423
             VS+MLSP FAK ++ KI D  TF P YY   W+Q+ DHGTSH  ++D +RNAVS+T+T
Sbjct: 329 KVVSDMLSPKFAKDLKSKINDQKTFDPKYYGGMWNQIDDHGTSHLSIIDRERNAVSMTST 388

Query: 424 VNEHFGAGILSPSTGIVLNNEMGDFSIP--TDISPDKLPPAPANFIQPNKRPLSSMTPII 483
           +N +FGA +LSPSTGIVLNNEM DFSIP  ++ + D  PPAPANFI+P KRPLSSM+P I
Sbjct: 389 INGYFGALMLSPSTGIVLNNEMDDFSIPMKSNGNLDVPPPAPANFIRPGKRPLSSMSPTI 448

Query: 484 VTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYENL 543
           V KD ++ A +G SGG  II    EV+LN+F L + PL++V   R+YHQLIPN   YEN 
Sbjct: 449 VLKDGKVKAAVGASGGANIIAGTTEVYLNHFFLKMDPLSSVLAPRIYHQLIPNRASYENW 508

Query: 544 TFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQS 603
           T +  DH E+P   +  LE +GH V++    G I QFIVQ      ++G   G  S    
Sbjct: 509 TTVFNDHFEIPKATRVVLEKKGH-VLSPIAGGTIAQFIVQ------ESGENSGGRSE--- 568

Query: 604 LFGLLTAVCDPRKNGFPA 620
               L AV DPRK GFP+
Sbjct: 569 ----LVAVSDPRKGGFPS 570

BLAST of Lcy08g018600 vs. TAIR 10
Match: AT4G39640.2 (gamma-glutamyl transpeptidase 1 )

HSP 1 Score: 535.8 bits (1379), Expect = 4.6e-152
Identity = 280/558 (50.18%), Postives = 371/558 (66.49%), Query Frame = 0

Query: 64  VESEKGVVAADDGRCSEIGASMLRRGGHAVDAAVATALCLGVVCSMSSGIGGGGFMLVRS 123
           +   +G VA DDGRCS IG  +LR GG+A+DA+VA ALCLGVV   SSGIGGG F +V+ 
Sbjct: 29  IVKSRGAVATDDGRCSVIGMRVLREGGNAIDASVAAALCLGVVSPASSGIGGGAFTVVKI 88

Query: 124 GLTLQTIALDFRETAPLAASQDMYETNVTAKHVGPLSVAVPGEIAGLHEAWLRYGRLAWK 183
               + IA D RETAPL A+++MY  NV  K  G LSV VPGE+AGL  AW ++G+L WK
Sbjct: 89  A-GGKEIAYDSRETAPLRATENMYGGNVDLKKKGALSVGVPGEVAGLFTAWKQHGKLPWK 148

Query: 184 SLFEPAIKIAKDGFVISPFLGKSLVSSAKLILNDPGLRQVFAPNGDLLQTGDTCYNVELG 243
            L  PA K+A +GF IS +L   + ++   IL D GL  +F  NG+L + G  C+N +L 
Sbjct: 149 RLVTPAEKLA-EGFKISKYLYMQMNATRSDILADKGLSDLFVSNGELKKPGTICHNPKLA 208

Query: 244 KSLEAVADQGPQVFYNGIVGEKLVKDVRAVGGILSMDDLKNYTVEVAEAMNTEAMGYTVH 303
            +L+ + + GP+ FYNG VG  L +D++  GGI+++ DL++Y V++ E ++ + +GY V 
Sbjct: 209 LTLKLIGEYGPKAFYNGTVGVNLARDIKKSGGIITLKDLQSYRVKIKEPLSADILGYRVL 268

Query: 304 GMPPPSSGTLGFAMVMNIFKSYNDPDAAKGNIGLHRLIEALKHMYAERMNLGDPRFSNIS 363
           GMPPPSSG     +V+NI   Y  P    G +G+HRLIEALKH +A RMNLGDP F++++
Sbjct: 269 GMPPPSSGGAAMMLVLNILSQYGIPSGVSGPLGVHRLIEALKHAFAVRMNLGDPDFTDVT 328

Query: 364 NSVSNMLSPSFAKKIQEKIIDNTTFPPDYYLYRWSQLRDHGTSHFCVVDADRNAVSLTTT 423
             VS+MLSP FAK ++ KI D  TF P YY   W+Q+ DHGTSH  ++D +RNAVS+T+T
Sbjct: 329 KVVSDMLSPKFAKDLKSKINDQKTFDPKYYGGMWNQIDDHGTSHLSIIDRERNAVSMTST 388

Query: 424 VNEHFGAGILSPSTGIVLNNEMGDFSIP--TDISPDKLPPAPANFIQPNKRPLSSMTPII 483
           +N +FGA +LSPSTGIVLNNEM DFSIP  ++ + D  PPAPANFI+P KRPLSSM+P I
Sbjct: 389 INGYFGALMLSPSTGIVLNNEMDDFSIPMKSNGNLDVPPPAPANFIRPGKRPLSSMSPTI 448

Query: 484 VTKDDRLIAVIGGSGGMKIIPAVIEVFLNYFSLGLQPLAAVQRSRVYHQLIPNVVKYENL 543
           V KD ++ A +G SGG  II    EV+LN+F L + PL++V   R+YHQLIPN   YEN 
Sbjct: 449 VLKDGKVKAAVGASGGANIIAGTTEVYLNHFFLKMDPLSSVLAPRIYHQLIPNRASYENW 508

Query: 544 TFIDGDHVELPDEKKQFLEDRGHQVVAIDTAGAIVQFIVQNFEEAIDTGRKGGKISNNQS 603
           T +  DH E+P   +  LE +GH V++    G I QFIVQ      ++G   G  S    
Sbjct: 509 TTVFNDHFEIPKATRVVLEKKGH-VLSPIAGGTIAQFIVQ------ESGENSGGRSE--- 568

Query: 604 LFGLLTAVCDPRKNGFPA 620
               L AV DPRK GFP+
Sbjct: 569 ----LVAVSDPRKGGFPS 570

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0G07.8e-21363.49Glutathione hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=GGT3 PE=2 SV=1[more]
Q680I52.3e-15650.44Glutathione hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=GGT2 PE=2 SV=1[more]
Q8VYW66.5e-15150.18Glutathione hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=GGT1 PE=2 SV=1[more]
Q9US047.8e-9640.59Glutathione hydrolase proenzyme 1 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
P0DPU32.5e-9436.51Scoloptoxin SSD14 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G2U80.0e+0085.97glutathione hydrolase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450289... [more]
A0A6J1DN402.3e-31084.06gamma-glutamyltranspeptidase 3 OS=Momordica charantia OX=3673 GN=LOC111021933 PE... [more]
A0A6J1KAW61.2e-30985.65glutathione hydrolase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493812 P... [more]
A0A5D3CHL73.0e-30884.38Gamma-glutamyltranspeptidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C9J32.5e-30784.22gamma-glutamyltranspeptidase 3 OS=Cucumis melo OX=3656 GN=LOC103498319 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038890419.10.0e+0086.63glutathione hydrolase 3 isoform X1 [Benincasa hispida][more]
XP_023521514.10.0e+0086.61glutathione hydrolase 3-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023547246.10.0e+0086.45glutathione hydrolase 3-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6599495.10.0e+0086.29Glutathione hydrolase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022946100.10.0e+0085.97glutathione hydrolase 3 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G29210.15.5e-21463.49gamma-glutamyl transpeptidase 4 [more]
AT4G29210.28.9e-17267.22gamma-glutamyl transpeptidase 4 [more]
AT4G39650.11.6e-15750.44gamma-glutamyl transpeptidase 2 [more]
AT4G39640.14.6e-15250.18gamma-glutamyl transpeptidase 1 [more]
AT4G39640.24.6e-15250.18gamma-glutamyl transpeptidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01210GGTRANSPTASEcoord: 91..116
score: 56.82
coord: 160..178
score: 50.24
coord: 405..423
score: 53.83
coord: 429..447
score: 50.48
coord: 178..197
score: 45.45
coord: 306..325
score: 32.05
coord: 276..292
score: 34.22
coord: 469..484
score: 59.94
coord: 493..510
score: 38.38
NoneNo IPR availablePFAMPF01019G_glu_transpeptcoord: 84..615
e-value: 4.7E-173
score: 576.6
NoneNo IPR availablePANTHERPTHR11686:SF61GAMMA-GLUTAMYLTRANSPEPTIDASE 1-RELATEDcoord: 22..620
IPR043138Gamma-glutamyltranspeptidase, large subunit, C-terminal domainGENE3D1.10.246.130coord: 292..397
e-value: 1.0E-28
score: 101.7
IPR000101Gamma-glutamyltranspeptidaseTIGRFAMTIGR00066TIGR00066coord: 69..571
e-value: 1.9E-121
score: 404.0
IPR000101Gamma-glutamyltranspeptidasePANTHERPTHR11686GAMMA GLUTAMYL TRANSPEPTIDASEcoord: 22..620
IPR043137Gamma-glutamyltranspeptidase, small subunitGENE3D3.60.20.40coord: 400..620
e-value: 8.3E-62
score: 210.4
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 63..618

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g018600.1Lcy08g018600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006751 glutathione catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0036374 glutathione hydrolase activity
molecular_function GO:0000048 peptidyltransferase activity