Lcy08g018440 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g018440
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description6-phosphogluconate dehydrogenase family protein
LocationChr08: 49178650 .. 49180261 (+)
RNA-Seq ExpressionLcy08g018440
SyntenyLcy08g018440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGTATGATACCTTTATGAATATTTTCACCTCTTCAATGTCAACCTCTTTAATTTGTCCCAAAAATGGAAGAAAATGGAGAAAATAATCCTCAAATAATTTAGGAAAAAAAACGAGATGCCACTGCAAGTCTCTCTTCGATCCCTACACACTCACTCCCACAAATTAATCCCCACCCTATCCCTTCCCTTCCTCTCTCTCCTCCGCCGCACCATGGCCACTTCCGGCGACATCGTCAGCCCTTCCACCACCCGCGTCGGCTGGATCGGCACCGGCGTCATGGGCTGCTCCATGTGTTCTCACATCATCAGAGCCGGCTTCAAACTCACCGTCTTCAATCGCACAATCTCCAAAGCCCAACCCCTTCTCGATTTGGGCGCCAATTTGGCCTCTTCCCCTCTCGCCGTCGCCGCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTCCTCCTCGACCCCACCTCCGGCGCCCTCGCCGGCCTCCGCCCAGGCGGCGTCCTCGTCGACATGACCACTTCCGAGCCCTCACTCGCCTCTGAAATCGCCGCCGCCGCTGCCGCCAAGGGCTGTGGGGCAATCGACGCTCCAGTCTCCGGCGGCGATCGCGGCGCCAAGAACGCCTCTCTGGCGATCTTCGCCGGCGGCGATGAAGCCCAAGTCCAGCGATTGAGCCCATTATTCTCGCTGTTGGGGAAGGTGAATTACATGGGGGAGAGTGGGAAAGGGCAGTTCGCGAAGCTGGCGAATCAGATCACCATAGCTTCGACGATGGTGGGGCTGGTGGAAGGGATGATTTACGCTCACAAAGCGGGGCTCGATGTGGGTTTGTTCTTGAACGCCATTTCAACAGGGGCTGCAGGTTCGAAGTCGCTGGATTTGTATGGGAGTAGGATTTTGAATAGGGATTTTGAACCTGGGTTTTTTGTGAATCATTTTGTGAAGGATTTGGGAATCTGTTTGAAGGAGTGTCAGAATATGGGGCTTGCTCTTCCTGGATTGGCATTGGCACAGCAGCTCTATGTGTCGCTTAAGGCTCATGGGGAGGGGAATTTGGGCACTCAGGCTCTGATTTTGTCCTTGGAACGGCTGAATAATGTTTCGCTCGAATCTGCCGCTGCCGGTGGCGGTGGTTCTTCTTGATGTGCCACCGTCTCTGCCGCCGTCTAGGTAGAAAGGCCGCTGATCCTTGATTCTAAGTGAGTATTTGATTCTGTTGTTGGGATTTAGGATAAGTTTGAAGGGTGAGTACATTGTGGTTGTTTTATGGCCATTTTGTTGTTACTACAAATTACAAATAAGAAACTTGTAAAGGTTTCTGTTCAAGCTTGGTTCAGTTCTTTGGGGAATTTTGTTTATAACTATTGCTTGTGGGCAGATGATTTATGAGGCGAATAAAGATTGGCTATGGCTTCCTACTAACTGGAAATTCTCAAGAAAATGACCAGAACTTTTCGTTTAATTCCTTGCTATTTCTTTCGAATTTTCAGTCACTTTGATCTCCACTCTCCTACATCGTTGGGTTCATAATGAAGAAAGAAAAATACTTAACTATATATTGATATGTGAGGATTTCTCTACACATCACTATTAA

mRNA sequence

ACAGTATGATACCTTTATGAATATTTTCACCTCTTCAATGTCAACCTCTTTAATTTGTCCCAAAAATGGAAGAAAATGGAGAAAATAATCCTCAAATAATTTAGGAAAAAAAACGAGATGCCACTGCAAGTCTCTCTTCGATCCCTACACACTCACTCCCACAAATTAATCCCCACCCTATCCCTTCCCTTCCTCTCTCTCCTCCGCCGCACCATGGCCACTTCCGGCGACATCGTCAGCCCTTCCACCACCCGCGTCGGCTGGATCGGCACCGGCGTCATGGGCTGCTCCATGTGTTCTCACATCATCAGAGCCGGCTTCAAACTCACCGTCTTCAATCGCACAATCTCCAAAGCCCAACCCCTTCTCGATTTGGGCGCCAATTTGGCCTCTTCCCCTCTCGCCGTCGCCGCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTCCTCCTCGACCCCACCTCCGGCGCCCTCGCCGGCCTCCGCCCAGGCGGCGTCCTCGTCGACATGACCACTTCCGAGCCCTCACTCGCCTCTGAAATCGCCGCCGCCGCTGCCGCCAAGGGCTGTGGGGCAATCGACGCTCCAGTCTCCGGCGGCGATCGCGGCGCCAAGAACGCCTCTCTGGCGATCTTCGCCGGCGGCGATGAAGCCCAAGTCCAGCGATTGAGCCCATTATTCTCGCTGTTGGGGAAGGTGAATTACATGGGGGAGAGTGGGAAAGGGCAGTTCGCGAAGCTGGCGAATCAGATCACCATAGCTTCGACGATGGTGGGGCTGGTGGAAGGGATGATTTACGCTCACAAAGCGGGGCTCGATGTGGGTTTGTTCTTGAACGCCATTTCAACAGGGGCTGCAGGTTCGAAGTCGCTGGATTTGTATGGGAGTAGGATTTTGAATAGGGATTTTGAACCTGGGTTTTTTGTGAATCATTTTGTGAAGGATTTGGGAATCTGTTTGAAGGAGTGTCAGAATATGGGGCTTGCTCTTCCTGGATTGGCATTGGCACAGCAGCTCTATGTGTCGCTTAAGGCTCATGGGGAGGGGAATTTGGGCACTCAGGCTCTGATTTTGTCCTTGGAACGGCTGAATAATGTTTCGCTCGAATCTGCCGCTGCCGGTGGCGGTGGTTCTTCTTGATGTGCCACCGTCTCTGCCGCCGTCTAGGTAGAAAGGCCGCTGATCCTTGATTCTAAGTGAGTATTTGATTCTGTTGTTGGGATTTAGGATAAGTTTGAAGGGTGAGTACATTGTGGTTGTTTTATGGCCATTTTGTTGTTACTACAAATTACAAATAAGAAACTTGTAAAGGTTTCTGTTCAAGCTTGGTTCAGTTCTTTGGGGAATTTTGTTTATAACTATTGCTTGTGGGCAGATGATTTATGAGGCGAATAAAGATTGGCTATGGCTTCCTACTAACTGGAAATTCTCAAGAAAATGACCAGAACTTTTCGTTTAATTCCTTGCTATTTCTTTCGAATTTTCAGTCACTTTGATCTCCACTCTCCTACATCGTTGGGTTCATAATGAAGAAAGAAAAATACTTAACTATATATTGATATGTGAGGATTTCTCTACACATCACTATTAA

Coding sequence (CDS)

ATGCCACTGCAAGTCTCTCTTCGATCCCTACACACTCACTCCCACAAATTAATCCCCACCCTATCCCTTCCCTTCCTCTCTCTCCTCCGCCGCACCATGGCCACTTCCGGCGACATCGTCAGCCCTTCCACCACCCGCGTCGGCTGGATCGGCACCGGCGTCATGGGCTGCTCCATGTGTTCTCACATCATCAGAGCCGGCTTCAAACTCACCGTCTTCAATCGCACAATCTCCAAAGCCCAACCCCTTCTCGATTTGGGCGCCAATTTGGCCTCTTCCCCTCTCGCCGTCGCCGCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTCCTCCTCGACCCCACCTCCGGCGCCCTCGCCGGCCTCCGCCCAGGCGGCGTCCTCGTCGACATGACCACTTCCGAGCCCTCACTCGCCTCTGAAATCGCCGCCGCCGCTGCCGCCAAGGGCTGTGGGGCAATCGACGCTCCAGTCTCCGGCGGCGATCGCGGCGCCAAGAACGCCTCTCTGGCGATCTTCGCCGGCGGCGATGAAGCCCAAGTCCAGCGATTGAGCCCATTATTCTCGCTGTTGGGGAAGGTGAATTACATGGGGGAGAGTGGGAAAGGGCAGTTCGCGAAGCTGGCGAATCAGATCACCATAGCTTCGACGATGGTGGGGCTGGTGGAAGGGATGATTTACGCTCACAAAGCGGGGCTCGATGTGGGTTTGTTCTTGAACGCCATTTCAACAGGGGCTGCAGGTTCGAAGTCGCTGGATTTGTATGGGAGTAGGATTTTGAATAGGGATTTTGAACCTGGGTTTTTTGTGAATCATTTTGTGAAGGATTTGGGAATCTGTTTGAAGGAGTGTCAGAATATGGGGCTTGCTCTTCCTGGATTGGCATTGGCACAGCAGCTCTATGTGTCGCTTAAGGCTCATGGGGAGGGGAATTTGGGCACTCAGGCTCTGATTTTGTCCTTGGAACGGCTGAATAATGTTTCGCTCGAATCTGCCGCTGCCGGTGGCGGTGGTTCTTCTTGA

Protein sequence

MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGSS
Homology
BLAST of Lcy08g018440 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.7e-135
Identity = 248/324 (76.54%), Postives = 284/324 (87.65%), Query Frame = 0

Query: 19  PTLSLPFLSLLRRTMAT---SGDIVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNR 78
           P+ S+     LRR+MA+   S DI++PS T++GWIGTGVMG SMC H+I+AG+ +TVFNR
Sbjct: 9   PSPSVVSSFFLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNR 68

Query: 79  TISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVL 138
           TISKAQ L+D+GAN+A SP +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GGVL
Sbjct: 69  TISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVL 128

Query: 139 VDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLF 198
           VDMTTSEPSLA EIA AA+ K C +IDAPVSGGD GAKN  L+IFAGGDE  V+RL PLF
Sbjct: 129 VDMTTSEPSLAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLF 188

Query: 199 SLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAG 258
           SL+GKVN+MG SGKGQFAKLANQITIASTM+GLVEG+IYAHKAGLDV  FL AISTGAAG
Sbjct: 189 SLMGKVNFMGTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAG 248

Query: 259 SKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHG 318
           SKS+DLYG RIL RDF+PGF+VNHFVKDLGICL ECQ MGLALPGLALAQQLY+SLKAHG
Sbjct: 249 SKSIDLYGDRILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHG 308

Query: 319 EGNLGTQALILSLERLNNVSLESA 340
           EG+LGTQAL+L+LERLNNVS++S+
Sbjct: 309 EGDLGTQALLLALERLNNVSVQSS 332

BLAST of Lcy08g018440 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 5.2e-73
Identity = 147/297 (49.49%), Postives = 199/297 (67.00%), Query Frame = 0

Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHII AG+ +TV+ R + K + L   GA +A+SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL PGGV VDMT+S+P LA EI A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 GAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+  +L IFAGGD   V+ LSP+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVN 278
           I  AS +VGL EG+++A KAGLD   +L A+  GAAGS  + L+G  I+ RD+    F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +       A+PG AL++QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

BLAST of Lcy08g018440 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.5e-69
Identity = 139/297 (46.80%), Postives = 195/297 (65.66%), Query Frame = 0

Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHI+ AG+ +TV+ R + K + L   G   A+SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL+PGGV VDMT+S+P LA EI A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 GAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+   L IFAGGD   V+ L+P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVN 278
           I + S M+GL EG+++A KAGLD   +L A+  GAAGS  + L+G  +  RD++   F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +      +A+PG AL +QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

BLAST of Lcy08g018440 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.8e-65
Identity = 133/279 (47.67%), Postives = 186/279 (66.67%), Query Frame = 0

Query: 47  VGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFS 106
           +G+IG GVMG SM SHI+  G  + V+ RT  KA+ +L  GA    +   ++ ++DV+ +
Sbjct: 5   IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIIT 64

Query: 107 IVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVS 166
           +VGYPSDV  V    ++G +   + G  L+DMTTS+PSLA +IA AA  K   A+DAPVS
Sbjct: 65  MVGYPSDVEEVYFG-SNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVS 124

Query: 167 GGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGK-VNYMGESGKGQFAKLANQITIASTM 226
           GGD GA+N +LAI  GG++   +   P+FSL+G+ + Y G +G GQ  K+ NQI IA+ M
Sbjct: 125 GGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAAGM 184

Query: 227 VGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLG 286
           +G+ E M YA K+GL+    L +I+TGAAGS SL     R+L  +FEPGF+V HF+KD+G
Sbjct: 185 IGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKDMG 244

Query: 287 ICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQAL 325
           I L+E + MG  +PGL+LA+ LY  L A GE N GTQ++
Sbjct: 245 IALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSI 282

BLAST of Lcy08g018440 vs. ExPASy Swiss-Prot
Match: O33730 (Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_1503 PE=3 SV=2)

HSP 1 Score: 170.2 bits (430), Expect = 4.0e-41
Identity = 107/287 (37.28%), Postives = 153/287 (53.31%), Query Frame = 0

Query: 46  RVGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKA-QPLLDLGANLASSPLAVAAQSDVV 105
           +V +IG GVMG  M  H+++ G  +TV+NRT +KA Q +   G   A +P   A   D+V
Sbjct: 3   KVAFIGLGVMGFPMAGHLVKQGHDVTVYNRTGAKATQWVEQYGGKKADTPKDAAQGQDIV 62

Query: 106 FSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAP 165
           F+ VG   D+R V+L    G + G+  G +LVD TT+   +A EIAA         +DAP
Sbjct: 63  FTCVGNDDDLRQVVLG-EHGIVHGMHAGAILVDHTTASADVAREIAAYIEPLNIAFLDAP 122

Query: 166 VSGGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGK-VNYMGESGKGQFAKLANQITIAS 225
           VSGG  GA+N +L +  GGD+A    + P+ S   +    +G  G GQ  K+ NQI IA 
Sbjct: 123 VSGGQAGAENGALTVMMGGDQAHFDTVKPVISAYSRCAELLGPVGAGQLTKMVNQICIAG 182

Query: 226 TMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKD 285
            + GL EG+ +A  AGLD    +  IS GAA S  ++     +    ++ GF ++   KD
Sbjct: 183 VVQGLAEGLHFAKSAGLDGLKVIEVISKGAAQSWQMENRYKTMWQGQYDFGFAIDWMRKD 242

Query: 286 LGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLER 331
           LGI L E +  G  LP  AL  Q Y  ++A       T +L+  LE+
Sbjct: 243 LGIALDEARRNGSHLPVAALVDQFYSEVQAMKGNRWDTSSLLARLEK 288

BLAST of Lcy08g018440 vs. ExPASy TrEMBL
Match: A0A6J1G2N8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111450245 PE=4 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.3e-175
Identity = 325/346 (93.93%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSL+T SHKL PTLSLPFL  LRRTMATSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA AAKGC A+DAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E +V+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGS 347
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL+SAA GG GS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 346

BLAST of Lcy08g018440 vs. ExPASy TrEMBL
Match: A0A6J1KJW1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493847 PE=4 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.2e-173
Identity = 323/346 (93.35%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSL+T SHKL PTLSL FL  LRRTMATSGDIV+P+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLAPTLSLSFLPHLRRTMATSGDIVNPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AGFKLTVFNRTISKAQPL+DLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLVDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC A+DAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E +V+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGS 347
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL+SAA GG GS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 346

BLAST of Lcy08g018440 vs. ExPASy TrEMBL
Match: A0A0A0LIQ8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 321/347 (92.51%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSL+T SHKL PTLSLPFLS LRRTM+TSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           P+SGALAGLRPGGVL+DMTTSEPSLASEIAA+A AKGCGAIDAPVSGGDRGAKNA+LAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGDE +VQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGSS 348
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSLE     G GSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-----GSGSS 342

BLAST of Lcy08g018440 vs. ExPASy TrEMBL
Match: A0A1S3C8W8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498120 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 320/347 (92.22%), Postives = 332/347 (95.68%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ +LRSL+T S KL PTLSLPFLS LRRTM+TSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AG+KLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAA AKGCGAIDAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGDE +V+RLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGSS 348
           LALAQQLYVSLKA+GEGNLGTQALILS+ERLNNVSL+   +   GSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGSRANGSS 347

BLAST of Lcy08g018440 vs. ExPASy TrEMBL
Match: A0A6J1DMH9 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021913 PE=4 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.4e-168
Identity = 314/341 (92.08%), Postives = 330/341 (96.77%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSLH HS     TLS+  +SLLRRTMATSGDI+SPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLHAHS----LTLSVQLVSLLRRTMATSGDILSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SH+IRAGFKLTVFNRTISKAQPLLDLGA+LASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHLIRAGFKLTVFNRTISKAQPLLDLGADLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGG+LVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGILVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGDE QV+RL+PLF+LLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGDEGQVRRLNPLFALLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRIL RDFE GFFVNHFVKDLGICLKECQNMG+ALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEAGFFVNHFVKDLGICLKECQNMGIALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAA 342
           LALAQQLY+SLKAHGEGNLGTQALILSLERLN+VSL+S+AA
Sbjct: 301 LALAQQLYLSLKAHGEGNLGTQALILSLERLNDVSLQSSAA 337

BLAST of Lcy08g018440 vs. NCBI nr
Match: XP_023545872.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.9 bits (1613), Expect = 2.0e-175
Identity = 327/346 (94.51%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSL+T SHKL PTLSLPFL  LRRTM TSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMTTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC A+DAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E +VQRL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVQRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGS 347
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL+SAA GG GS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAAGGGSGS 346

BLAST of Lcy08g018440 vs. NCBI nr
Match: XP_022946035.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 622.9 bits (1605), Expect = 1.7e-174
Identity = 325/346 (93.93%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSL+T SHKL PTLSLPFL  LRRTMATSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAA AAKGC A+DAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E +V+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGS 347
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL+SAA GG GS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 346

BLAST of Lcy08g018440 vs. NCBI nr
Match: XP_022999508.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 619.0 bits (1595), Expect = 2.5e-173
Identity = 323/346 (93.35%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSL+T SHKL PTLSL FL  LRRTMATSGDIV+P+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLAPTLSLSFLPHLRRTMATSGDIVNPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AGFKLTVFNRTISKAQPL+DLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLVDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC A+DAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGG+E +V+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGS 347
           LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSL+SAA GG GS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 346

BLAST of Lcy08g018440 vs. NCBI nr
Match: XP_038889572.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 614.4 bits (1583), Expect = 6.1e-172
Identity = 322/347 (92.80%), Postives = 334/347 (96.25%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MPLQ+SLRSL+T S++L  TLSLPFLS LR TM+TS DIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSNQLTHTLSLPFLSFLRCTMSTSRDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSD+VFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDIVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAA AKGCGAIDAPVSGGDRGAKNASLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGDE++V+RL PLFSLLGKVNYMGE+GKGQFAKLANQITIASTMVGLVEGMIYAHKAGL
Sbjct: 181 AGGDESEVRRLKPLFSLLGKVNYMGETGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGSS 348
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSLESA  G GG S
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLESAPGGDGGRS 347

BLAST of Lcy08g018440 vs. NCBI nr
Match: XP_004141022.2 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis sativus] >KGN60577.1 hypothetical protein Csa_019202 [Cucumis sativus])

HSP 1 Score: 611.7 bits (1576), Expect = 4.0e-171
Identity = 321/347 (92.51%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MPLQVSLRSLHTHSHKLIPTLSLPFLSLLRRTMATSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSL+T SHKL PTLSLPFLS LRRTM+TSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHII+AG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIF 180
           P+SGALAGLRPGGVL+DMTTSEPSLASEIAA+A AKGCGAIDAPVSGGDRGAKNA+LAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240
           AGGDE +VQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRIL RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLESAAAGGGGSS 348
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSLE     G GSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-----GSGSS 342

BLAST of Lcy08g018440 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.4e-136
Identity = 248/324 (76.54%), Postives = 284/324 (87.65%), Query Frame = 0

Query: 19  PTLSLPFLSLLRRTMAT---SGDIVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNR 78
           P+ S+     LRR+MA+   S DI++PS T++GWIGTGVMG SMC H+I+AG+ +TVFNR
Sbjct: 9   PSPSVVSSFFLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNR 68

Query: 79  TISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVL 138
           TISKAQ L+D+GAN+A SP +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GGVL
Sbjct: 69  TISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVL 128

Query: 139 VDMTTSEPSLASEIAAAAAAKGCGAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLF 198
           VDMTTSEPSLA EIA AA+ K C +IDAPVSGGD GAKN  L+IFAGGDE  V+RL PLF
Sbjct: 129 VDMTTSEPSLAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLF 188

Query: 199 SLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAG 258
           SL+GKVN+MG SGKGQFAKLANQITIASTM+GLVEG+IYAHKAGLDV  FL AISTGAAG
Sbjct: 189 SLMGKVNFMGTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAG 248

Query: 259 SKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHG 318
           SKS+DLYG RIL RDF+PGF+VNHFVKDLGICL ECQ MGLALPGLALAQQLY+SLKAHG
Sbjct: 249 SKSIDLYGDRILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHG 308

Query: 319 EGNLGTQALILSLERLNNVSLESA 340
           EG+LGTQAL+L+LERLNNVS++S+
Sbjct: 309 EGDLGTQALLLALERLNNVSVQSS 332

BLAST of Lcy08g018440 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.7e-74
Identity = 147/297 (49.49%), Postives = 199/297 (67.00%), Query Frame = 0

Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHII AG+ +TV+ R + K + L   GA +A+SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL PGGV VDMT+S+P LA EI A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 GAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+  +L IFAGGD   V+ LSP+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVN 278
           I  AS +VGL EG+++A KAGLD   +L A+  GAAGS  + L+G  I+ RD+    F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +       A+PG AL++QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

BLAST of Lcy08g018440 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 263.1 bits (671), Expect = 3.2e-70
Identity = 139/297 (46.80%), Postives = 195/297 (65.66%), Query Frame = 0

Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHI+ AG+ +TV+ R + K + L   G   A+SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL+PGGV VDMT+S+P LA EI A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 GAIDAPVSGGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+   L IFAGGD   V+ L+P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 ITIASTMVGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVN 278
           I + S M+GL EG+++A KAGLD   +L A+  GAAGS  + L+G  +  RD++   F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +      +A+PG AL +QL+  + A+G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

BLAST of Lcy08g018440 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 143.7 bits (361), Expect = 2.8e-34
Identity = 100/320 (31.25%), Postives = 164/320 (51.25%), Query Frame = 0

Query: 19  PTLSLPFLSLLRRTMATSGDIVSPS------TTRVGWIGTGVMGCSMCSHIIRAGFKLTV 78
           P LS P + L  R + +S    +PS      T  +G++G G+MG  M  ++I+AG  +TV
Sbjct: 36  PFLSKPQICLAYR-VYSSLQSTTPSTRDELGTVSIGFLGMGIMGSPMAQNLIKAGCDVTV 95

Query: 79  FNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPG 138
           +NRT SK  PL+ LGA   SSP  V A  D+ F+++  P     V     +GA+ G+  G
Sbjct: 96  WNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFAMLADPESAIDVACG-KNGAIFGISSG 155

Query: 139 GVLVDMTTSEPSLASEIAAAAAAKGCGA--IDAPVSGGDRGAKNASLAIFAGGDEAQVQR 198
              VD++T +  +AS I  +   K  GA  ++APVSG  + A++  L     GD+   ++
Sbjct: 156 KGYVDVSTVD--VASSILISKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLYEK 215

Query: 199 LSPLFSLLGKVN-YMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGLDVGLFLNAI 258
            +P   ++GK   Y+GE G G   KL   + + S M    EG++ + K GLD  + +  +
Sbjct: 216 AAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVEVV 275

Query: 259 STGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYV 318
           S GA  +    L G  ++   +   F + H  KD+ + L   +++  + P  A A +LY 
Sbjct: 276 SQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANELYK 335

Query: 319 SLKAHGEGNLGTQALILSLE 330
             K++G  +    A+I +L+
Sbjct: 336 VAKSYGLSDEDFSAVIEALK 351

BLAST of Lcy08g018440 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 133.3 bits (334), Expect = 3.8e-31
Identity = 85/280 (30.36%), Postives = 144/280 (51.43%), Query Frame = 0

Query: 47  VGWIGTGVMGCSMCSHIIRAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFS 106
           VG++G G+MG +M  ++++ GFK+TV+NRT+SK   L++ GA++  SP  V  +     +
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 107 IVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCGAIDAPVS 166
           ++  P    SV+ D   G L  +  G   +DM+T +   + +I  A   KG   ++ PVS
Sbjct: 63  MLSDPCAALSVVFD-KGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVS 122

Query: 167 GGDRGAKNASLAIFAGGDEAQVQRLSPLFSLLGKVN-YMGESGKGQFAKLANQITIASTM 226
           G  + A++  L I A GD+A  +   P F +LGK + Y+G+ G G   KL   + + S M
Sbjct: 123 GSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMM 182

Query: 227 VGLVEGMIYAHKAGLDVGLFLNAISTGAAGSKSLDLYGSRILNRDFEPGFFVNHFVKDLG 286
               EG++ A K+GL     L+ +  GA  +      G  +    + P F + H  KD+ 
Sbjct: 183 NAFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMR 242

Query: 287 ICLKECQNMGLALPGLALAQQLYVSLKAHGEGNLGTQALI 326
           + L       +++P  A A + +   ++ G G+L   A+I
Sbjct: 243 LALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVI 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZE14.7e-13576.54Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis... [more]
Q949M85.2e-7349.49Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis... [more]
Q9C9914.5e-6946.80Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis... [more]
O349488.8e-6547.67Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ... [more]
O337304.0e-4137.28Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCI... [more]
Match NameE-valueIdentityDescription
A0A6J1G2N88.3e-17593.93probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m... [more]
A0A6J1KJW11.2e-17393.35probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m... [more]
A0A0A0LIQ81.9e-17192.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1[more]
A0A1S3C8W81.9e-17192.22probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis mel... [more]
A0A6J1DMH93.4e-16892.08probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Momordica c... [more]
Match NameE-valueIdentityDescription
XP_023545872.12.0e-17594.51probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pep... [more]
XP_022946035.11.7e-17493.93probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita mos... [more]
XP_022999508.12.5e-17393.35probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita max... [more]
XP_038889572.16.1e-17292.80probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa his... [more]
XP_004141022.24.0e-17192.51probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis sativ... [more]
Match NameE-valueIdentityDescription
AT4G29120.13.4e-13676.546-phosphogluconate dehydrogenase family protein [more]
AT1G71180.13.7e-7449.496-phosphogluconate dehydrogenase family protein [more]
AT1G71170.13.2e-7046.806-phosphogluconate dehydrogenase family protein [more]
AT1G17650.12.8e-3431.25glyoxylate reductase 2 [more]
AT3G25530.13.8e-3130.36glyoxylate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0158153-hydroxyisobutyrate dehydrogenase-relatedPIRSFPIRSF000103HIBADHcoord: 41..336
e-value: 5.3E-60
score: 200.9
NoneNo IPR availableGENE3D3.40.50.720coord: 40..205
e-value: 1.0E-46
score: 160.7
NoneNo IPR availablePANTHERPTHR430603-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATEDcoord: 39..334
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 46..202
e-value: 5.2E-42
score: 143.7
IPR0291543-hydroxyisobutyrate dehydrogenase, NAD-binding domainPFAMPF14833NAD_binding_11coord: 208..325
e-value: 1.8E-32
score: 112.1
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 206..335
e-value: 3.5E-37
score: 129.6
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 206..330
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 45..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g018440.1Lcy08g018440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0016491 oxidoreductase activity