Lcy08g018390 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g018390
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationChr08: 49129742 .. 49134318 (-)
RNA-Seq ExpressionLcy08g018390
SyntenyLcy08g018390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCATCAGTGCCACTCTGTTCATAATGCACTGCAATTGCTTTTCAATTGCCAATATCCGCCGCTAGAAATTTCGTATGTTCCATTCCACTTTACTCATAATGAACTTGATTGATTTGTTTTTGAACTTTTTGCTCTGTTTTCTGTCGAATCGTTCTTTTCGCCGATCCTGATGGTGTGTTGTTCCATTTTTGTTGAGCAATAGGAGGGGGAGCTATCGACGTATTATGAAATTCCTCGGAAAACATGGAAAATCCGACCAAAATTGATCCGAATCCGAAATTCTTGGCTCTCAATTGTATCGACCTTTCCAGTTCAGATGTTCATCAATCGGTTTCGTTACTCAAACAGGTTATCTGTAATATGATGATCGTATTAGTTGATTTCCAGTTTCGGTTTGTGTTCTGTGTAGAAATCGAAGTGGAACTTCTGTTTGGTGATTGGAGAAGTTTTTTGTTCTTTCAGGCCTGTTTGGATACCGGATTCTTTTACGCTGTGAATCATGGAATAACGGAGGAATTTATGCATGAGGTTTTTGCTCAGAGCAAAAGGCTTTTCAGTCTGCCACTGAGTGATAAAATGAAGCTTCTGCGAAACGAGAAGCATAGAGGCTACACTCCGATTCTCGACGAACTGCTTGATCCTGATAATCAAATACGAGGTATATTTTGTGCAATTCGTCTATCTTCCTTGATTGCTTAGAATTTAATGTTCTTCATTACACATTGTTGTTTGTAGGGGATTACAAGGAGGGGTATTATATAGGAATGGAGGAAAAAATTCCTGGAGAACATCCAGATGGAGAAAGGCCATTTTATGGTCCAAATATTTGGCCTGCAGAAGGTATATCATGAAATTACTTATTGTTGCTTTACCAAATATAGTATGACTTCTTGCTCAATAAATTTTGCTGTTTAGATTTGTTACCTGGCTGGAAGGAGATCATGGAGAAGTTTCATAGACAAGCACTGTATGTTCTGGTTCTGGTGATTATACAAATTTATTATGATATTTCTAACAAATTATTATTTAATCTCTTTTGGTGGTCGTACAAAATTTGTTTGTTTGTTTGAGTGAATGGTGACAGAGTTGTCTTTTTGTAAGTCTGCAAAACGTCCAATCACTGGTGAAAAGAGTTCTGTCAATTCTTCTCCCATTTTTATATTCTAGTCTCAAATCCTGACTAAATACTCCTGATCTGGACTCTCGAATAAGAAATTGGCATAACCTATCACCCGATGGAAAGGCCAATCAGTTCTAGAAACATTCGTACTATTCAATTGGCTTCTTGCTATCAATTATTATGTAATATGCCTTTTAAAAATCTTGGTCTTAATTTTTTGATATTTGGCCTTTTCTGATAACTTGCATAACCCAATGGATTAGTTAAAAGAAAAATTGTGTTTGCCATCCTCTACTCACCCCTCACCACTCTATGAACATGACAAACTCATTTGTATCTTGACTTTTTTTTAAAATGTGAAAATCAGAGAGGTTGCTAGAGTAATTGCAAGGCTCATTGCTCTTGCACTTGGTCTTGAGAATGATTTTTTTGACAAGTCAGAAATGCTCGGTGAGCCTATTGCAGTCTTGCGCCTACTACACTATGAAGGTGGGATATTAACACTTTTAGGCCCCGTTTGGTAACCATTTGGTTTTTGGTTTTTGGTTTTTCAAAATTATACTTGTTTTCTCTCAATTTCCTTACTATAGTTTTCATCTTGGTTAAGGATACATTGGAGTTTTTAGCCAAATTCCAAAAACAAAAACAAGTTTTTGAAATCATGACAATTAGGTAGGTTCAAACCAAAGAAATGGATAGGTAGAATGAGTGTTTATAGGCTTAATTTTCAAAAACAAAAAACGAAATGGTTATCAAACGGGGCCTTAGTTACTTCACTTTTGGCCCTGTGGCGTATGTTGGCCATAGAAATAATAAAAGAGTCCTCTTTATGCATCAGGCCAAATTTCTGAACCTGCGAAAGGAATATTTGGAGCTGGAGCTCATACAGACTATGGTTTAATCACACTCTTGGCGACAGATGAGAACCTTGGTCTACAAGTAAGAACTTCCGGTTTGCGCACTCGTGCTGTTTAACCTTTATCAGCATGTCACATTATTTGATTAATCATTGAAAGTGTTTGTAATTAGTGCAGATTTGCAAGGATAAAGATGCTAAACCTCAAATATGGGAATATGTAGCACCACTCAAAGGGTGAGTTTTATGGAATCATTAGGAACTAGTTTAGATGCTTGATATCATTTTAATTCGGTATTCTCGCACATGTGAAGTTTGAAAAGATGTGACTATAGCACAGAATCCTTCAGTGTGCATCATTTTACTCATGTTCTAAAATTTCACTTCAGAGTTGAATAAATGATAATAACAGAGAAGGGAGTCAACAAAAGATTGTTTTCTGGCATCTTGATTTTAGTCTGTTTGGTTTTTAACAGAGCGTTTGTGGTGAACCTTGGAGACATGCTGGAGCGCTGGAGCAACTGCATATTCAAGTAAGATCCATTGTCCTGTTTTACACAACCATTTCATTTGGCCCTTTAATATTATCTATTCTAATTTACATCCATTAGTATGAGGTTACCTTTTTTTATTGGTTTTATATTTTGACTACCTCATGGAAAATGAAGCCCCATCCAAAAAAAAAAACTATGAATTTAGTTAGTAGGTGCATAATAAGATTTCTGACAGGTGAAGGTTTTTAACCCTTCTCCTCATTTATCTATTTATATAAAACCTCGCTAAAATTATCAATGGATATGTTGAAGCATGATGAACTTTCAAATGTGATATTCTCAGATATTGTATGAACTTGTATGTTCCAAATATGCTTCAATATGTTATAGTGTATTATTTATTTGCCTTCTCATCTGCCTTCAAGAATCTTGAATGGCTATGTCGTTTTCGGCTTTCATGAGGAATTTATTTGCATCTTATGTTAAAACCAAAGTAGTAAACAAGATCAACCATCTAATTATAGATACTTCAGTACCTCTTTTTTAAATAAAAAAACATTTTGGATGTGACATAATCCCAAGTTCCTAACACTTTTTCTTTTTTGATTTGATCTCAGGTCCACATTACATAGAGTTCTGGTTAATGGTCAAGATAGATATTCTGTAAGCCAATTGCAACCGTTACCCTTAGCTTGATCTGTTATAAACATCTCATGGCAATACTCTTCATAATCATCGGTCCCACTATTTACAAATATACAAACAAACAAGAACAATATTTGGCTGCATACTGGTATGTAGGGAGATCCCTATCCCTACATACCCCATTAAAAATTCGAAAACTAAGAAAGAGAAAGAGAGAAAATGAGAAAACTTTTTTATCTCTCGTAATTGTCAATATTTTAATGGTCCATGTTACTTGGAATCCCATCTGATTACTGATACTAAATTTTTTTTTTTCATGAGAGCAGATTGCATATTTTGTGGAACCTAGTCACGACTGTCTTGTCGAATGTTTGCCAACCTGCACGTCAGAAAAAAACCCTCCCAAGTAAGTAGATATTGATATAAGTTTTTCTATTGTCTACCAAATATATATATATATATATGCACATGAGATTTTCTAAAGTTTGCACATATTGAGGCAAGTGCCTCACAGGCGAGCTTTCGTTGCTTGCAGGGAGTAGTGTCAATTGGCAGCTCACTGTTTTCCTTTCTTTTTGCTTTTTTTTTTTTTTTTTTTTGTCAGGTTTCCTCCAATCTTATGCAGCACATATTTGAGCCAGCGTTACAAAGAAACTCATGTTGATCTAAATACATATAATAATGAAGATAAAAAATACAGTTGACATGGCCTAGGAAGTTGCATACTCTTGCAAGGAGCAATGGCATGCTCCAATGTTATGAAAATAGTAATGGTTGTAATTACAATAAAGTCACTTGTAATGCCAAAGTCTAACAGGGACAGGCAGACGGTGGATGGCTGAGGGCAGACCAAAATTTCAGGCCGGATCAAAAGTTGGGCAGTGTTATTCACTCCAGAGCTTTAATTATTATTTATAAAGTGAAAGTAATTTTCATGTCATCTCGACTTTATAGCGTTTAATATTTAATCTTTTCACTCGAAATTTAATCCCTGTGATCGAACATATTTTTTTTTTTACAATTTTTTTGTTTTGCTTAAAAATTGTTTTTCTCATTTTTTAGTGTGTATATTCTGGCAAGCAATTTATTTTTAGAGACAAAAAATATTGACCCTTTCAAAATCACAAATTTGTGTGTTGATTTTATTGCATATAACATTAACTTCTATGCAGGGTGTCAAATTTCGGTCGACATTTCGAGGGGGAAGTTTCGTTCTTCCCCCATATCGTCAACATCTCGAAATGTTGAAGATGTTCTACGAACCTTAAAATTTTTAAAGTAAACGGAATAGCTTTTGGAACCTCCTCCAAGATCGTCAAAGAGTTTTTTCTATAGTTACTATTCTTGGGGGTTTAAATTGTCACTCATGGATCCAGCTTTTTGGTCTAGAAACGTCTTAGAAATTTAATCCAATATTTTATCATGAGGGAGTTGACTCATGTATCATATATGCCAATTT

mRNA sequence

CTTTCATCAGTGCCACTCTGTTCATAATGCACTGCAATTGCTTTTCAATTGCCAATATCCGCCGCTAGAAATTTCGAGGGGGAGCTATCGACGTATTATGAAATTCCTCGGAAAACATGGAAAATCCGACCAAAATTGATCCGAATCCGAAATTCTTGGCTCTCAATTGTATCGACCTTTCCAGTTCAGATGTTCATCAATCGGTTTCGTTACTCAAACAGGCCTGTTTGGATACCGGATTCTTTTACGCTGTGAATCATGGAATAACGGAGGAATTTATGCATGAGGTTTTTGCTCAGAGCAAAAGGCTTTTCAGTCTGCCACTGAGTGATAAAATGAAGCTTCTGCGAAACGAGAAGCATAGAGGCTACACTCCGATTCTCGACGAACTGCTTGATCCTGATAATCAAATACGAGGGGATTACAAGGAGGGGTATTATATAGGAATGGAGGAAAAAATTCCTGGAGAACATCCAGATGGAGAAAGGCCATTTTATGGTCCAAATATTTGGCCTGCAGAAGATTTGTTACCTGGCTGGAAGGAGATCATGGAGAAGTTTCATAGACAAGCACTAGAGGTTGCTAGAGTAATTGCAAGGCTCATTGCTCTTGCACTTGGTCTTGAGAATGATTTTTTTGACAAGTCAGAAATGCTCGGTGAGCCTATTGCAGTCTTGCGCCTACTACACTATGAAGGCCAAATTTCTGAACCTGCGAAAGGAATATTTGGAGCTGGAGCTCATACAGACTATGGTTTAATCACACTCTTGGCGACAGATGAGAACCTTGGTCTACAAATTTGCAAGGATAAAGATGCTAAACCTCAAATATGGGAATATGTAGCACCACTCAAAGGAGCGTTTGTGGTGAACCTTGGAGACATGCTGGAGCGCTGGAGCAACTGCATATTCAAGTCCACATTACATAGAGTTCTGGTTAATGGTCAAGATAGATATTCTGTAAGCCAATTGCAACCGTTACCCTTAGCTTGATCTGTTATAAACATCTCATGGCAATACTCTTCATAATCATCGGTCCCACTATTTACAAATATACAAACAAACAAGAACAATATTTGGCTGCATACTGGTATGTAGGGAGATCCCTATCCCTACATACCCCATTAAAAATTCGAAAACTAAGAAAGAGAAAGAGAGAAAATGAGAAAACTTTTTTATCTCTCGTAATTGTCAATATTTTAATGGTCCATGTTACTTGGAATCCCATCTGATTACTGATACTAAATTTTTTTTTTTCATGAGAGCAGATTGCATATTTTGTGGAACCTAGTCACGACTGTCTTGTCGAATGTTTGCCAACCTGCACGTCAGAAAAAAACCCTCCCAAGTTTCCTCCAATCTTATGCAGCACATATTTGAGCCAGCGTTACAAAGAAACTCATGTTGATCTAAATACATATAATAATGAAGATAAAAAATACAGTTGACATGGCCTAGGAAGTTGCATACTCTTGCAAGGAGCAATGGCATGCTCCAATGTTATGAAAATAGTAATGGTTGTAATTACAATAAAGTCACTTGTAATGCCAAAGTCTAACAGGGACAGGCAGACGGTGGATGGCTGAGGGCAGACCAAAATTTCAGGCCGGATCAAAAGTTGGGCAGTGTTATTCACTCCAGAGCTTTAATTATTATTTATAAAGTGAAAGTAATTTTCATGTCATCTCGACTTTATAGCGTTTAATATTTAATCTTTTCACTCGAAATTTAATCCCTGTGATCGAACATATTTTTTTTTTTACAATTTTTTTGTTTTGCTTAAAAATTGTTTTTCTCATTTTTTAGTGTGTATATTCTGGCAAGCAATTTATTTTTAGAGACAAAAAATATTGACCCTTTCAAAATCACAAATTTGTGTGTTGATTTTATTGCATATAACATTAACTTCTATGCAGGGTGTCAAATTTCGGTCGACATTTCGAGGGGGAAGTTTCGTTCTTCCCCCATATCGTCAACATCTCGAAATGTTGAAGATGTTCTACGAACCTTAAAATTTTTAAAGTAAACGGAATAGCTTTTGGAACCTCCTCCAAGATCGTCAAAGAGTTTTTTCTATAGTTACTATTCTTGGGGGTTTAAATTGTCACTCATGGATCCAGCTTTTTGGTCTAGAAACGTCTTAGAAATTTAATCCAATATTTTATCATGAGGGAGTTGACTCATGTATCATATATGCCAATTT

Coding sequence (CDS)

ATGGAAAATCCGACCAAAATTGATCCGAATCCGAAATTCTTGGCTCTCAATTGTATCGACCTTTCCAGTTCAGATGTTCATCAATCGGTTTCGTTACTCAAACAGGCCTGTTTGGATACCGGATTCTTTTACGCTGTGAATCATGGAATAACGGAGGAATTTATGCATGAGGTTTTTGCTCAGAGCAAAAGGCTTTTCAGTCTGCCACTGAGTGATAAAATGAAGCTTCTGCGAAACGAGAAGCATAGAGGCTACACTCCGATTCTCGACGAACTGCTTGATCCTGATAATCAAATACGAGGGGATTACAAGGAGGGGTATTATATAGGAATGGAGGAAAAAATTCCTGGAGAACATCCAGATGGAGAAAGGCCATTTTATGGTCCAAATATTTGGCCTGCAGAAGATTTGTTACCTGGCTGGAAGGAGATCATGGAGAAGTTTCATAGACAAGCACTAGAGGTTGCTAGAGTAATTGCAAGGCTCATTGCTCTTGCACTTGGTCTTGAGAATGATTTTTTTGACAAGTCAGAAATGCTCGGTGAGCCTATTGCAGTCTTGCGCCTACTACACTATGAAGGCCAAATTTCTGAACCTGCGAAAGGAATATTTGGAGCTGGAGCTCATACAGACTATGGTTTAATCACACTCTTGGCGACAGATGAGAACCTTGGTCTACAAATTTGCAAGGATAAAGATGCTAAACCTCAAATATGGGAATATGTAGCACCACTCAAAGGAGCGTTTGTGGTGAACCTTGGAGACATGCTGGAGCGCTGGAGCAACTGCATATTCAAGTCCACATTACATAGAGTTCTGGTTAATGGTCAAGATAGATATTCTGTAAGCCAATTGCAACCGTTACCCTTAGCTTGA

Protein sequence

MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVSQLQPLPLA
Homology
BLAST of Lcy08g018390 vs. ExPASy Swiss-Prot
Match: Q1ERI0 (2-oxoglutarate-dependent dioxygenase mpl2 OS=Monascus purpureus OX=5098 GN=mpl2 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.5e-36
Identity = 95/268 (35.45%), Postives = 144/268 (53.73%), Query Frame = 0

Query: 20  DLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRN 79
           D +S    + +  ++ AC  TGFF  + HGI+      VFA + + F+LP SD     RN
Sbjct: 22  DPNSDAARKVIDDVRAACTSTGFFQLLGHGISPALQQSVFAAAAKFFALP-SDVKSRCRN 81

Query: 80  EKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLP 139
              RGY P+  +  +    +  D KEG+  G +  +       +R F G N WP  +LLP
Sbjct: 82  VGFRGYDPMASQSYELG--VLPDLKEGFIAGKDIPLDDPRVASQRFFMGQNAWPPSELLP 141

Query: 140 --GWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHY-EGQI 199
              ++  +E++++  L++  V+  L+A  L      FD+ +   +P   LRLLHY     
Sbjct: 142 EANFRRPIEEYYQAMLKLCWVVLDLVAATLPYGPHVFDEFKE-NDPACPLRLLHYPPAPA 201

Query: 200 SEPAKG-IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGD 259
            + AKG   G+ AHTD+G ITLL  D++ GL++   +D +   W  V P K A+VVNLGD
Sbjct: 202 PDVAKGRQLGSSAHTDFGAITLLLQDDHSGLEV---QDCETGEWIGVPPNKDAYVVNLGD 261

Query: 260 MLERWSNCIFKSTLHRVL-VNGQDRYSV 283
           M+ R +   +KS++HRV+  N  DRYSV
Sbjct: 262 MMSRITRGHYKSSIHRVINQNLTDRYSV 282

BLAST of Lcy08g018390 vs. ExPASy Swiss-Prot
Match: A0A159BP93 (2-oxoglutarate-dependent dioxygenase citB OS=Monascus ruber OX=89489 GN=citB PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.5e-36
Identity = 95/268 (35.45%), Postives = 144/268 (53.73%), Query Frame = 0

Query: 20  DLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRN 79
           D +S    + +  ++ AC  TGFF  + HGI+      VFA + + F+LP SD     RN
Sbjct: 22  DPNSDAARKVIDDVRAACTSTGFFQLLGHGISPALQQSVFAAAAKFFALP-SDVKSRCRN 81

Query: 80  EKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLP 139
              RGY P+  +  +    +  D KEG+  G +  +       +R F G N WP  +LLP
Sbjct: 82  VGFRGYDPMASQSYELG--VLPDLKEGFIAGKDIPLDDPRVASQRFFMGQNAWPPSELLP 141

Query: 140 --GWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHY-EGQI 199
              ++  +E++++  L++  V+  L+A  L      FD+ +   +P   LRLLHY     
Sbjct: 142 EANFRRPIEEYYQAMLKLCWVVLDLVAATLPYGPHVFDEFKE-NDPACPLRLLHYPPAPA 201

Query: 200 SEPAKG-IFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGD 259
            + AKG   G+ AHTD+G ITLL  D++ GL++   +D +   W  V P K A+VVNLGD
Sbjct: 202 PDVAKGRQLGSSAHTDFGAITLLLQDDHSGLEV---QDCETGEWIGVPPNKDAYVVNLGD 261

Query: 260 MLERWSNCIFKSTLHRVL-VNGQDRYSV 283
           M+ R +   +KS++HRV+  N  DRYSV
Sbjct: 262 MMSRITRGHYKSSIHRVINQNLTDRYSV 282

BLAST of Lcy08g018390 vs. ExPASy Swiss-Prot
Match: C8VK14 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=hxnY PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.5e-36
Identity = 100/262 (38.17%), Postives = 145/262 (55.34%), Query Frame = 0

Query: 30  VSLLKQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLR--NEKHRGYTP 89
           V  ++++C   GFF    H I  E    V   +KR F+LPL +KM + +  N  +RGY  
Sbjct: 26  VQQVRESCEYNGFFQITGHRIPRELQVRVMDAAKRFFALPLEEKMAIDKNLNSFNRGYEL 85

Query: 90  ILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP--DGERPFYGPNIWPAEDLLPGWKEIM 149
           +  ++L+       + KEG YIG  E+I  +HP     R   GPN WPA   +P  +E  
Sbjct: 86  LRSQMLEVGT--APELKEGLYIG--EEIGADHPYYINGRLNSGPNQWPA--TVPDAQEFR 145

Query: 150 E---KFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQ-ISEPAK 209
           E   +++    E+A+ +  ++AL L +E  FFD     G  +A +R+LHY  Q   E  K
Sbjct: 146 ETSMEYYHAVYELAKDVLAVLALTLDVEESFFDPLTEGG--VATMRMLHYPSQPKDEDEK 205

Query: 210 GIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWS 269
              G GAHTD+G ITLL  DE  GLQ+    DA    W  V P+ GA+VVNLGD++ R +
Sbjct: 206 LNRGIGAHTDFGCITLLLQDEVDGLQVL---DAPSGQWLDVQPVLGAYVVNLGDLMMRMA 265

Query: 270 NCIFKSTLHRVL-VNGQDRYSV 283
           N  +KS +HRV+  +G++RYS+
Sbjct: 266 NDRYKSNIHRVINKSGRERYSI 276

BLAST of Lcy08g018390 vs. ExPASy Swiss-Prot
Match: Q6K332 (2-oxoglutarate-dependent dioxygenase 33 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD33 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.7e-35
Identity = 94/269 (34.94%), Postives = 142/269 (52.79%), Query Frame = 0

Query: 21  LSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDKMKLLRNE 80
           ++  D+ Q V +L  AC + GFFY   HGI +  M +V   +++ F LP  +K+K+    
Sbjct: 26  VNDEDLLQVVQMLDDACREAGFFYVKGHGIADSLMKQVRDVTQKFFQLPYEEKLKIKMTP 85

Query: 81  K--HRGYTPILDELL--DPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAED 140
           +  +RGY  + + +    PD     D     Y  +E   PG++ D  +P  G N+WP   
Sbjct: 86  QSGYRGYQRVGENITKGKPDMHEAID----CYTPIE---PGKYGDLAKPMVGSNLWPKYP 145

Query: 141 LLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHY--EG 200
               +  ++E +     +++R I R IALALG   D F+     G+P  V RL+ Y    
Sbjct: 146 --SNFDVLLENYISLLRDLSRKIMRGIALALGAPVDAFE-GTTAGDPFWVCRLIGYPVST 205

Query: 201 QISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLG 260
            I E  +   G GAHTDYGL+TL+  D+++     +++  +   W Y  P+ G FV N+G
Sbjct: 206 DIPEEQRTDTGCGAHTDYGLLTLVNQDDDICALEVRNQSGE---WIYAKPIPGTFVCNIG 265

Query: 261 DMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           DML+ WSN I++ TLHRV VN   RY VS
Sbjct: 266 DMLKVWSNGIYQPTLHRV-VNNSPRYRVS 280

BLAST of Lcy08g018390 vs. ExPASy Swiss-Prot
Match: A0A5C1RDG7 (2-oxoglutarate-dependent dioxygenase OS=Didymella fabae OX=372025 GN=orf3 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.0e-34
Identity = 89/269 (33.09%), Postives = 141/269 (52.42%), Query Frame = 0

Query: 22  SSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFAQSKRLFSLPLSDK--MKLLRN 81
           +S    Q V  +++AC  TGFF    HG++++ + ++FA S + F+LP   K  +   +N
Sbjct: 24  TSPSGQQVVDQVRKACTSTGFFQLTGHGLSQDVVEDIFAASAKFFALPKDVKQGLNFTKN 83

Query: 82  EKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWPAEDLLP 141
              RGY  I  ++ + D  +  D KEG+  G++          +R F G N+WP  D+LP
Sbjct: 84  LGFRGYELIGAQVYESD--VLPDLKEGFLAGIDLPFEDMRVQNKRFFAGQNVWPPPDVLP 143

Query: 142 --GWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYEGQIS 201
              ++E +EK+++  +++   +  L+A  L    D FD+ +   EP   LRLLHY    +
Sbjct: 144 YAEFREPVEKYYKSIMQLCFTVMDLVAATLPYGPDVFDEWKS-HEPACPLRLLHYPPTPA 203

Query: 202 EPAKGI--FGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGD 261
             A      G+ AHTD+G +TLL  D + GL++          W  V P  GAFVVN+ D
Sbjct: 204 HVAGKTRQLGSSAHTDFGALTLLLQDSHEGLEVLNHDTGD---WVLVPPKPGAFVVNIAD 263

Query: 262 MLERWSNCIFKSTLHRVLVNG--QDRYSV 283
           M+   +   +KS+ HRV+     +DRYSV
Sbjct: 264 MMTMVTVGEYKSSKHRVINRNETEDRYSV 286

BLAST of Lcy08g018390 vs. ExPASy TrEMBL
Match: A0A6J1G3R7 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.7e-149
Identity = 255/283 (90.11%), Postives = 268/283 (94.70%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T  DP+P F ALNCIDLSSSD+H SVSLLKQACLDTGFFYAVNHGI EEFM EVFA
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIG+EEKIPG+  
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
            GE PFYGPNIWPAEDLLPGW++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQ+WE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYS++
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIA 283

BLAST of Lcy08g018390 vs. ExPASy TrEMBL
Match: A0A6J1KDA3 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.5e-148
Identity = 254/283 (89.75%), Postives = 266/283 (93.99%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLKQACLDTGFFYAVNHGI EEFM EVFA
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSKRLFSLPL DKMKLLRNEKHRGYTPILDELLDP+NQIRGDYKEGYYIG+EEK PG+  
Sbjct: 61  QSKRLFSLPLCDKMKLLRNEKHRGYTPILDELLDPENQIRGDYKEGYYIGIEEKNPGDDS 120

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
            GE PFYGPNIWPAEDLLPGW++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDEN GLQICKDKDAKP+IWE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKDAKPRIWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYS++
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIA 283

BLAST of Lcy08g018390 vs. ExPASy TrEMBL
Match: A0A6J1G3P3 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.3e-148
Identity = 255/284 (89.79%), Postives = 268/284 (94.37%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T  DP+P F ALNCIDLSSSD+H SVSLLKQACLDTGFFYAVNHGI EEFM EVFA
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEH 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR GDYKEGYYIG+EEKIPG+ 
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKIPGDD 120

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
             GE PFYGPNIWPAEDLLPGW++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEM
Sbjct: 121 SGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEM 180

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQ+W
Sbjct: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           EYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYS++
Sbjct: 241 EYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIA 284

BLAST of Lcy08g018390 vs. ExPASy TrEMBL
Match: A0A0A0LIR5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000940 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.8e-147
Identity = 249/283 (87.99%), Postives = 267/283 (94.35%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MENPT+ DPNP+FLALNCIDLSSSDV+ SVSLLKQACLDTGFFY VNHGITEEFM EVFA
Sbjct: 1   MENPTENDPNPEFLALNCIDLSSSDVNLSVSLLKQACLDTGFFYVVNHGITEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSKRLF LPLSDKMKLLRNE HRGYTPILDELLDP+NQIRGDYKEGYYIG+EE  PG+  
Sbjct: 61  QSKRLFDLPLSDKMKLLRNENHRGYTPILDELLDPENQIRGDYKEGYYIGIEETNPGDGS 120

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
           DG+RPFYGPN+WPAED+LP W +IMEKFH QALEVARV+ARLIALALGLE +FFDKSEML
Sbjct: 121 DGKRPFYGPNVWPAEDMLPDWSKIMEKFHAQALEVARVVARLIALALGLEKEFFDKSEML 180

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           G+P A LRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQ+W+
Sbjct: 181 GKPTATLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQVWQ 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLV+GQDRYS++
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVSGQDRYSIA 283

BLAST of Lcy08g018390 vs. ExPASy TrEMBL
Match: A0A6J1KFK8 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.1e-147
Identity = 254/284 (89.44%), Postives = 266/284 (93.66%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLKQACLDTGFFYAVNHGI EEFM EVFA
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEH 120
           QSKRLFSLPL DKMKLLRNEKHRGYTPILDELLDP+NQIR GDYKEGYYIG+EEK PG+ 
Sbjct: 61  QSKRLFSLPLCDKMKLLRNEKHRGYTPILDELLDPENQIRVGDYKEGYYIGIEEKNPGDD 120

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
             GE PFYGPNIWPAEDLLPGW++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEM
Sbjct: 121 SGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEM 180

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDEN GLQICKDKDAKP+IW
Sbjct: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKDAKPRIW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           EYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYS++
Sbjct: 241 EYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIA 284

BLAST of Lcy08g018390 vs. NCBI nr
Match: XP_038877416.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X2 [Benincasa hispida])

HSP 1 Score: 547.7 bits (1410), Expect = 5.9e-152
Identity = 259/283 (91.52%), Postives = 272/283 (96.11%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLKQACLDTGFFYAVNHGITEEFM EVFA
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 87

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIG+EEK PG+  
Sbjct: 88  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKNPGDDS 147

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
           DGERPFYGPNIWPAEDLLPGW++IMEKFH QALEVAR IARLIALAL LENDFFDK+E L
Sbjct: 148 DGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDLENDFFDKAETL 207

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           GEPIA+LRLLHYEGQ+SEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW+
Sbjct: 208 GEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWQ 267

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYS++
Sbjct: 268 YVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIA 310

BLAST of Lcy08g018390 vs. NCBI nr
Match: XP_038877418.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X4 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 259/284 (91.20%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLKQACLDTGFFYAVNHGITEEFM EVFA
Sbjct: 1   MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEH 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR GDYKEGYYIG+EEK PG+ 
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKNPGDD 120

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
            DGERPFYGPNIWPAEDLLPGW++IMEKFH QALEVAR IARLIALAL LENDFFDK+E 
Sbjct: 121 SDGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDLENDFFDKAET 180

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LGEPIA+LRLLHYEGQ+SEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW
Sbjct: 181 LGEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           +YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYS++
Sbjct: 241 QYVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIA 284

BLAST of Lcy08g018390 vs. NCBI nr
Match: XP_038877415.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X1 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 259/284 (91.20%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLKQACLDTGFFYAVNHGITEEFM EVFA
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 87

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEH 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR GDYKEGYYIG+EEK PG+ 
Sbjct: 88  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKNPGDD 147

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
            DGERPFYGPNIWPAEDLLPGW++IMEKFH QALEVAR IARLIALAL LENDFFDK+E 
Sbjct: 148 SDGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDLENDFFDKAET 207

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LGEPIA+LRLLHYEGQ+SEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW
Sbjct: 208 LGEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 267

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           +YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYS++
Sbjct: 268 QYVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIA 311

BLAST of Lcy08g018390 vs. NCBI nr
Match: XP_038877417.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X3 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 259/284 (91.20%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MENPT+ D NPKFLALNCIDLSSSDV++SVSLLKQACLDTGFFYAVNHGITEEFM EVFA
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 87

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR-GDYKEGYYIGMEEKIPGEH 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIR GDYKEGYYIG+EEK PG+ 
Sbjct: 88  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKNPGDD 147

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
            DGERPFYGPNIWPAEDLLPGW++IMEKFH QALEVAR IARLIALAL LENDFFDK+E 
Sbjct: 148 SDGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDLENDFFDKAET 207

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LGEPIA+LRLLHYEGQ+SEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW
Sbjct: 208 LGEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 267

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           +YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYS++
Sbjct: 268 QYVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIA 311

BLAST of Lcy08g018390 vs. NCBI nr
Match: XP_023545999.1 (2-oxoglutarate-dependent dioxygenase mpl2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.8 bits (1392), Expect = 7.2e-150
Identity = 257/283 (90.81%), Postives = 268/283 (94.70%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T  DPNP F ALNCIDLSSSD+H SVSLLKQACLDTGFFYAVNHGI EEFM EVFA
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIG+EEKIPG+  
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
            GE PFYGPNIWPAEDLLPGW++IMEKFHR+ALEVAR IARLIALALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDEN GLQICKDK+AKPQIWE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQIWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSVS 284
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYS++
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIA 283

BLAST of Lcy08g018390 vs. TAIR 10
Match: AT1G35190.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 9.8e-113
Identity = 187/282 (66.31%), Postives = 234/282 (82.98%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T +    K  +LNCIDL++ D++ SV  LKQACLD GFFY +NHGI+EEFM +VF 
Sbjct: 1   MENHTTM----KVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFE 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSK+LF+LPL +KMK+LRNEKHRGYTP+LDELLDP NQI GD+KEGYYIG+E  +P + P
Sbjct: 61  QSKKLFALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIE--VPKDDP 120

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
             ++PFYGPN WP  D+LPGW+E MEK+H++AL V+  IARL+ALAL L+  +FD++EML
Sbjct: 121 HWDKPFYGPNPWPDADVLPGWRETMEKYHQEALRVSMAIARLLALALDLDVGYFDRTEML 180

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           G+PIA +RLL Y+G IS+P+KGI+  GAH+D+G++TLLATD  +GLQICKDK+A PQ WE
Sbjct: 181 GKPIATMRLLRYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSV 283
           YV P+KGAF+VNLGDMLERWSN  FKSTLHRVL NGQ+RYS+
Sbjct: 241 YVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSI 275

BLAST of Lcy08g018390 vs. TAIR 10
Match: AT3G46490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 372.5 bits (955), Expect = 3.2e-103
Identity = 173/283 (61.13%), Postives = 221/283 (78.09%), Query Frame = 0

Query: 1   MENPTKIDPN-PKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVF 60
           MEN T+ + +  K  +L CIDL +SD+HQS  LLKQACLD+GFFY +NHGI+EE   E F
Sbjct: 1   MENKTEEEVSIIKVSSLTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAF 60

Query: 61  AQSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEH 120
             SK+ F+LPL +KMK+LRNEK+RGY P  D LLDP+NQ+RGDYKEG+ IG E    G H
Sbjct: 61  EHSKKFFALPLEEKMKVLRNEKYRGYAPFHDSLLDPENQVRGDYKEGFTIGFEGSKDGPH 120

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
            D  +PF+ PNIWP  D+LPGW+E MEK++++AL V + IA+++ALAL L+ D+F+  EM
Sbjct: 121 WD--KPFHSPNIWPNPDVLPGWRETMEKYYQEALRVCKSIAKIMALALDLDVDYFNTPEM 180

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LG PIA + L HYEG+ S+P+KGI+  GAH+D+G+++LLATD  +GLQICKDKD KPQ W
Sbjct: 181 LGNPIADMVLFHYEGK-SDPSKGIYACGAHSDFGMMSLLATDGVMGLQICKDKDVKPQKW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSV 283
           EY   +KGA++VNLGD+LERWSN  FKSTLHRVL NGQDRYS+
Sbjct: 241 EYTPSIKGAYIVNLGDLLERWSNGYFKSTLHRVLGNGQDRYSI 280

BLAST of Lcy08g018390 vs. TAIR 10
Match: AT1G35190.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 4.6e-102
Identity = 175/282 (62.06%), Postives = 217/282 (76.95%), Query Frame = 0

Query: 1   MENPTKIDPNPKFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVFA 60
           MEN T +    K  +LNCIDL++ D++ SV  LKQACLD GFFY +NHGI+EEFM +VF 
Sbjct: 1   MENHTTM----KVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFE 60

Query: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHP 120
           QSK+LF+LPL +KMK+LRNEKHRGYTP+LDELLDP NQI GD+KEGYYIG+E  +P + P
Sbjct: 61  QSKKLFALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIE--VPKDDP 120

Query: 121 DGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEML 180
             ++PFYGPN WP  D+LPGW+E MEK+H++AL                       +EML
Sbjct: 121 HWDKPFYGPNPWPDADVLPGWRETMEKYHQEAL-----------------------TEML 180

Query: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWE 240
           G+PIA +RLL Y+G IS+P+KGI+  GAH+D+G++TLLATD  +GLQICKDK+A PQ WE
Sbjct: 181 GKPIATMRLLRYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSV 283
           YV P+KGAF+VNLGDMLERWSN  FKSTLHRVL NGQ+RYS+
Sbjct: 241 YVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSI 252

BLAST of Lcy08g018390 vs. TAIR 10
Match: AT3G46480.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 8.9e-82
Identity = 151/283 (53.36%), Postives = 193/283 (68.20%), Query Frame = 0

Query: 1   MENPTKIDPNP-KFLALNCIDLSSSDVHQSVSLLKQACLDTGFFYAVNHGITEEFMHEVF 60
           MEN T+ + +  K  +L CIDL++ +  QS   LK                         
Sbjct: 1   MENKTQEEASTIKVSSLTCIDLANPNFQQSAVSLK------------------------- 60

Query: 61  AQSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEH 120
            QSK+ F+LPL +KMK+LRNEKHRGY+P+LD++LDP+NQ+ GDYKE ++IG+E       
Sbjct: 61  -QSKKFFALPLEEKMKVLRNEKHRGYSPVLDQILDPENQVDGDYKESFFIGIEV------ 120

Query: 121 PDGERPFYGPNIWPAEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEM 180
                            +LPGW+  MEK+H++AL V + IARL+ALAL L+ ++FDK EM
Sbjct: 121 -----------------VLPGWRATMEKYHQEALRVCKAIARLLALALDLDTNYFDKPEM 180

Query: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIW 240
           LG PIAV+RLL YEG +S+P KGIFG GAH+DYG++TLLATD   GLQICKDKD KP+ W
Sbjct: 181 LGNPIAVMRLLRYEG-MSDPLKGIFGCGAHSDYGMLTLLATDSVTGLQICKDKDVKPRKW 233

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSV 283
           EYV  +KGA++VNLGD+LERWSN IFKSTLHRVL NGQDRYS+
Sbjct: 241 EYVPSIKGAYIVNLGDLLERWSNGIFKSTLHRVLGNGQDRYSI 233

BLAST of Lcy08g018390 vs. TAIR 10
Match: AT3G46500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 271.9 bits (694), Expect = 5.8e-73
Identity = 123/215 (57.21%), Postives = 166/215 (77.21%), Query Frame = 0

Query: 74  MKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGMEEKIPGEHPDGERPFYGPNIWP 133
           MK+L+NEKH+GY+P+L ++   DNQI GDYKE ++IG+E          + PF   NIWP
Sbjct: 1   MKVLKNEKHQGYSPVLSQI--SDNQIHGDYKESFFIGIE-------GSNDTPFCRANIWP 60

Query: 134 AEDLLPGWKEIMEKFHRQALEVARVIARLIALALGLENDFFDKSEMLGEPIAVLRLLHYE 193
             D+L GW+  MEK+H++AL V + IAR++ALAL ++ D+FD  EMLG P+  +RLLHYE
Sbjct: 61  NPDVLSGWQATMEKYHQEALRVCKAIARVLALALNVDGDYFDTPEMLGNPLTFMRLLHYE 120

Query: 194 GQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNL 253
           G +S+P+KGI+G G H+D+G++TLL TD  +GLQICKD+D KP+ WEY+  +KGA++VN+
Sbjct: 121 G-MSDPSKGIYGCGPHSDFGMMTLLGTDSVMGLQICKDRDVKPRKWEYILSIKGAYIVNI 180

Query: 254 GDMLERWSNCIFKSTLHRVLVNGQDRYSVS-QLQP 288
           GD+LERWSN IFKSTLHRVL NGQDRYS++  LQP
Sbjct: 181 GDLLERWSNGIFKSTLHRVLGNGQDRYSIAFFLQP 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1ERI02.5e-3635.452-oxoglutarate-dependent dioxygenase mpl2 OS=Monascus purpureus OX=5098 GN=mpl2 ... [more]
A0A159BP932.5e-3635.452-oxoglutarate-dependent dioxygenase citB OS=Monascus ruber OX=89489 GN=citB PE=... [more]
C8VK145.5e-3638.172-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FG... [more]
Q6K3322.7e-3534.942-oxoglutarate-dependent dioxygenase 33 OS=Oryza sativa subsp. japonica OX=39947... [more]
A0A5C1RDG71.0e-3433.092-oxoglutarate-dependent dioxygenase OS=Didymella fabae OX=372025 GN=orf3 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A6J1G3R71.7e-14990.112-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1KDA32.5e-14889.752-oxoglutarate-Fe(II) type oxidoreductase-like isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1G3P34.3e-14889.792-oxoglutarate-dependent dioxygenase mpl2-like isoform X1 OS=Cucurbita moschata ... [more]
A0A0A0LIR52.8e-14787.99Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G... [more]
A0A6J1KFK86.1e-14789.442-oxoglutarate-Fe(II) type oxidoreductase-like isoform X1 OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
XP_038877416.15.9e-15291.522-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X2 [Benincasa hispid... [more]
XP_038877418.11.4e-15091.202-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X4 [Benincasa hispid... [more]
XP_038877415.11.4e-15091.202-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X1 [Benincasa hispid... [more]
XP_038877417.11.4e-15091.202-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X3 [Benincasa hispid... [more]
XP_023545999.17.2e-15090.812-oxoglutarate-dependent dioxygenase mpl2 isoform X2 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
AT1G35190.19.8e-11366.312-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46490.13.2e-10361.132-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G35190.24.6e-10262.062-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46480.18.9e-8253.362-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46500.15.8e-7357.212-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 158..174
score: 55.08
coord: 125..141
score: 39.57
coord: 33..50
score: 59.6
coord: 249..275
score: 29.97
NoneNo IPR availablePANTHERPTHR10209:SF7682OG-FE(II) OXYGENASE FAMILY OXIDOREDUCTASEcoord: 15..284
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 15..284
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 15..282
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 185..282
e-value: 9.5E-20
score: 71.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 17..135
e-value: 3.8E-30
score: 105.1
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 7..285
e-value: 2.0E-79
score: 269.5
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 183..289
score: 10.000404

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g018390.1Lcy08g018390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding