Lcy08g017430 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g017430
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description(1->3)-beta-glucan endohydrolase
LocationChr08: 48152654 .. 48156152 (-)
RNA-Seq ExpressionLcy08g017430
SyntenyLcy08g017430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAAATCAAAACCCCTATTACTCACTCAGAAAACGACCCTCCTAAAACCCTTTTCACATCTCTCACTCTCCCTTTCTCTCTCATGAAAATTCTTCTCTGCCAACAGAGTTTGCATAAGGTTTTGGACAAATTAGAGAAAAGGGTCTTTGTCTATTTGTTCTAATTTTGTTGGGTCGTCGTCTTCTTCCTCATGCCATTTCAGTGGAATGATTAGTAGAAACTGTGGGACGTTTTGAAAGAAATCTGCTGTTTCTGCTTGGTTTCTAAGCCATCTGATCGAATTCCCATTTCCATATCTCTTGGAAGTTCGATTTTCCTTGGCGATTTTCTTCTATCAATGGCTAGGAAATGCTTATGGTTCTGTGTTCAATCGGTTCCTGGTTTCTGGGTCGTCTTCTCTGCCATTGGTACTGTAATTTTCTTCTCTCTTTCTCTCTGTTTCCCATTTCTTAATGTTTTGTAATAAGATGGTTTTGGGTTTTGGGTTTTGGGTTTTGAGTTTTGGGTTCCTTTTAACACTTGAATGGTTTTCTTTTTCATTTCCTCTCCTCTAGATCGATCGGTAGTATTATCTTTTTGTTTTGTTTGTTCCATTTTCTCTAAAGATTGCTTCCAGTTGACTTACAGTCCGAAGTTTTTATGGATCTTCCTCCATCTCTTTGCTTGATTTAGTCAGCGAGGTCAATTTATCATATTGGAAAGTGACAACCAGAATTCTTTAGAATATGTTTCTGACTGGCAAGTTTTTAGATTGTTCTTGACTTTGTGCCTGCATCAATTTTTTATTTGATGGTCTATTTTGACCGATTGACATGTTCTGTTTCAAATTGGAAAGAGTTATTTTCCGGGTTAGATTTGATTCTGTGATGCGCTTCTCTCATTCTTGGAGATTTTGTTGAGTACTGAGAGTAATTTTGACGTGTGTTTTACAAATTTGAGGTTTCTTGTTAAGTAATCCACTTGAGAAAGTTGTAGTAAGATGTCTCTTTGTTTTTCTTCAGCCTCCTTGACAATGACTGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCTGATGAAGTTGTTTCGCTTCTCAAAGCGGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTAAAGGCGTTTAATGGGACGGGGCTTGAATTGGTGGTCGGTCTACCGAATGGCTTCTTGAAAGAAATGAGTGCGAGCGAGGAACACGCCATGAACTGGGTCAAAGAAAATGTCCAGGCATTCCTTCCTCAGACTCACATTTGTGGGATTGCCATTGGAAATGAAGTTCTGGGAGGAGCTGACTTTGAGTTGTGGGGAGCACTCTTGGGTGCTGCAGAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCGTTCAGATCACGACAGCACATTCTCAGGCTGTTTTTTCGAACTCATTTCCGCCTTCTTCGTGTATATTTAGAGAAAACGTTGTTCAGTACATGAAGCCTTTGTTGGAGTTTTTTTCAGAGATTGGCTCACCTTTCTGTTTAAACGCTTACCCCTTTCTAGCCTATATGAGTGACCCGGAGAATATTGATATTAACTATGCACTTTTTGAATCAACTGAGGGGATTTTTGATCCTAAAACTAGTCTACATTATGACAACATGCTCGATGCTCAGATCGACGCTGCATATGCAGCACTGGCAGATGCTGGATATCAGAAAATGGAAGTCATTGTTACAGAGACTGGTTGGGCTTCCCGTGGGGACGAAAACGAAGCTGCAGCAACGGCGAATAATGCTAGGATATTTAATTACAACCTCCGGAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACATTTAACGAAAATTTGAAACCAGGGCCGACTTCTGAGAGAAATTTTGGGCTTTTCAAAGCTGATGGGACCATATCATATGACATTGGATTTCATGGACTTAAATCTTCATCTTCAGATTCATCTTATTTATCTCTCAAGGTATGTAATTCATCACAGTATATGAAGAATGTTATGTTTGTTGAAACTATGCTTTTCCCTTCCCTTGTCCAAATTAATTGATTTAACATGATTAATGGCAAAACAAAAGCTGGTATTCTTGAGCATCATACTTTGGGAGATCGAAGCTTTTATTGTACTGGATTCCACTAGAACTTAGAAGCTTACTGGAGTTCCTTCTAACCCGTTTGAAAGGTTGTTATCAAGACAAATACCTATTTTTAAGAACAAAATTTCTGTGGAAACGTATTTGAATAGCTTTTTAGTTAAATTACAACTTCAGTCCCATACATCGAAGTTTGTGTCAAATATGTCTTTAAACTTTAAAAAGTAGCTAATAGGTCTCTAAACTCTCGATTTTGTGTTTAATAGGTCTTTGAATTTTAAAAATGTCTGCTAAGTCTTCAAACTTCCAGTCTTGTGTCTAACAAGTCCCTGAACTTTCAGTTTTGTGTCTATTCGACATTTTTTTAAATTCACAGACATATTGGACACAATAATGAAAGTTTATTGTCCTATTAGACACAAAATTCAATTTTATGTCCAATAAAAGTTCTTGAATTTTAACAAATGTTGAATATGCTAGAGACCTATTAGACGCAAACATGAAAGTTTATTGTCATATAAAAAAAAAACGATGAATATGCTAGGGATCTATTAGACAGAAAATTGAAACTTTAAGAACCTATTTGACATTTTTAAAGTCCATGAACTTATTAGTCATAAAATTAAAAGTTCAAGAACCTAATACTTTATAAAGTTTAGAGACCTATTAGACACAACATAAAAATTAAGGGACTGAAAATGTAAGTTAACCTAACGTTTTCTAATACAATGTTTATTTTCAATTACAGAAGTGTTTTCTATCAATAGAAAACAAAACAAATAAGACTGGGGTTATCAAGAAAAGAAACTACTTTATTTCATCGAAAAAAGAAAAGAAACTACTTTATCTTTTAGAAACGAAAAATCAAGTTGATATTGTTACTTAGCAACGTCAAAAGCCATTGTATTCCAATCCAGCATCTTTCTTAGGTTGTACTAAAGACCATCTTTATTGATATCCATACACATTCTGCTTGCTTATGTGAACAGAATCTCCGAGCTTTCTCGTTAACGATCTCCGCCGTGACGCTACTTCTATTGTTGAGATGAAAATGGAATGTAGAATGGTCTGAGAACTTATTGTGTTAAGTTAGGAAGGCAAACCTGCAGCAGCTGTTGGAAAGCATTGCATCTTCTGCTGGTACATGCCATACTTGGTACAGTTGCTGTTTATTCTTTTGCCCCTTGCATAATTGAACTCTCCATTTCCACCCACACCCCTTCTTTAAATTGTATCCCAATTTTCCACAAGGTTGAGTTTGAGATGGAACTATTGAATCATAAATGGAAGTGGCAAAATAAAAGCAAAATATGCATATGATTCCCAG

mRNA sequence

CCCAAATCAAAACCCCTATTACTCACTCAGAAAACGACCCTCCTAAAACCCTTTTCACATCTCTCACTCTCCCTTTCTCTCTCATGAAAATTCTTCTCTGCCAACAGAGTTTGCATAAGGTTTTGGACAAATTAGAGAAAAGGGTCTTTGTCTATTTGTTCTAATTTTGTTGGGTCGTCGTCTTCTTCCTCATGCCATTTCAGTGGAATGATTAGTAGAAACTGTGGGACGTTTTGAAAGAAATCTGCTGTTTCTGCTTGGTTTCTAAGCCATCTGATCGAATTCCCATTTCCATATCTCTTGGAAGTTCGATTTTCCTTGGCGATTTTCTTCTATCAATGGCTAGGAAATGCTTATGGTTCTGTGTTCAATCGGTTCCTGGTTTCTGGGTCGTCTTCTCTGCCATTGCCTCCTTGACAATGACTGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCTGATGAAGTTGTTTCGCTTCTCAAAGCGGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTAAAGGCGTTTAATGGGACGGGGCTTGAATTGGTGGTCGGTCTACCGAATGGCTTCTTGAAAGAAATGAGTGCGAGCGAGGAACACGCCATGAACTGGGTCAAAGAAAATGTCCAGGCATTCCTTCCTCAGACTCACATTTGTGGGATTGCCATTGGAAATGAAGTTCTGGGAGGAGCTGACTTTGAGTTGTGGGGAGCACTCTTGGGTGCTGCAGAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCGTTCAGATCACGACAGCACATTCTCAGGCTGTTTTTTCGAACTCATTTCCGCCTTCTTCGTGTATATTTAGAGAAAACGTTGTTCAGTACATGAAGCCTTTGTTGGAGTTTTTTTCAGAGATTGGCTCACCTTTCTGTTTAAACGCTTACCCCTTTCTAGCCTATATGAGTGACCCGGAGAATATTGATATTAACTATGCACTTTTTGAATCAACTGAGGGGATTTTTGATCCTAAAACTAGTCTACATTATGACAACATGCTCGATGCTCAGATCGACGCTGCATATGCAGCACTGGCAGATGCTGGATATCAGAAAATGGAAGTCATTGTTACAGAGACTGGTTGGGCTTCCCGTGGGGACGAAAACGAAGCTGCAGCAACGGCGAATAATGCTAGGATATTTAATTACAACCTCCGGAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACATTTAACGAAAATTTGAAACCAGGGCCGACTTCTGAGAGAAATTTTGGGCTTTTCAAAGCTGATGGGACCATATCATATGACATTGGATTTCATGGACTTAAATCTTCATCTTCAGATTCATCTTATTTATCTCTCAAGGTTGTACTAAAGACCATCTTTATTGATATCCATACACATTCTGCTTGCTTATGTGAACAGAATCTCCGAGCTTTCTCGTTAACGATCTCCGCCGTGACGCTACTTCTATTGTTGAGATGAAAATGGAATGTAGAATGGTCTGAGAACTTATTGTGTTAAGTTAGGAAGGCAAACCTGCAGCAGCTGTTGGAAAGCATTGCATCTTCTGCTGGTACATGCCATACTTGGTACAGTTGCTGTTTATTCTTTTGCCCCTTGCATAATTGAACTCTCCATTTCCACCCACACCCCTTCTTTAAATTGTATCCCAATTTTCCACAAGGTTGAGTTTGAGATGGAACTATTGAATCATAAATGGAAGTGGCAAAATAAAAGCAAAATATGCATATGATTCCCAG

Coding sequence (CDS)

ATGGCTAGGAAATGCTTATGGTTCTGTGTTCAATCGGTTCCTGGTTTCTGGGTCGTCTTCTCTGCCATTGCCTCCTTGACAATGACTGTACAAGCATTTACTGGAACTTATGGAATAAATTATGGAAGGATTGCTGATAACATTCCTTCACCTGATGAAGTTGTTTCGCTTCTCAAAGCGGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTAAAGGCGTTTAATGGGACGGGGCTTGAATTGGTGGTCGGTCTACCGAATGGCTTCTTGAAAGAAATGAGTGCGAGCGAGGAACACGCCATGAACTGGGTCAAAGAAAATGTCCAGGCATTCCTTCCTCAGACTCACATTTGTGGGATTGCCATTGGAAATGAAGTTCTGGGAGGAGCTGACTTTGAGTTGTGGGGAGCACTCTTGGGTGCTGCAGAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCGTTCAGATCACGACAGCACATTCTCAGGCTGTTTTTTCGAACTCATTTCCGCCTTCTTCGTGTATATTTAGAGAAAACGTTGTTCAGTACATGAAGCCTTTGTTGGAGTTTTTTTCAGAGATTGGCTCACCTTTCTGTTTAAACGCTTACCCCTTTCTAGCCTATATGAGTGACCCGGAGAATATTGATATTAACTATGCACTTTTTGAATCAACTGAGGGGATTTTTGATCCTAAAACTAGTCTACATTATGACAACATGCTCGATGCTCAGATCGACGCTGCATATGCAGCACTGGCAGATGCTGGATATCAGAAAATGGAAGTCATTGTTACAGAGACTGGTTGGGCTTCCCGTGGGGACGAAAACGAAGCTGCAGCAACGGCGAATAATGCTAGGATATTTAATTACAACCTCCGGAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACATTTAACGAAAATTTGAAACCAGGGCCGACTTCTGAGAGAAATTTTGGGCTTTTCAAAGCTGATGGGACCATATCATATGACATTGGATTTCATGGACTTAAATCTTCATCTTCAGATTCATCTTATTTATCTCTCAAGGTTGTACTAAAGACCATCTTTATTGATATCCATACACATTCTGCTTGCTTATGTGAACAGAATCTCCGAGCTTTCTCGTTAACGATCTCCGCCGTGACGCTACTTCTATTGTTGAGATGA

Protein sequence

MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLKSSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR
Homology
BLAST of Lcy08g017430 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.0e-88
Identity = 158/337 (46.88%), Postives = 227/337 (67.36%), Query Frame = 0

Query: 23  IASLTMTVQAFTGT-YGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFNG 82
           + SLT+ +   T T  G+NYG+I DN+PSP +V+ L+K+     V++YDA+ ++LKAF+ 
Sbjct: 14  LISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSN 73

Query: 83  TGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWG 142
           TG+E ++GL N +L +M      A+ W+K+NV  FLP T+I  I IGNE+L   D  L  
Sbjct: 74  TGIEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTT 133

Query: 143 ALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFS 202
            LL A + ++ A+    L   + +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  
Sbjct: 134 NLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHR 193

Query: 203 EIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAAL 262
           +  SPF +NAYPF AY  +P+ + +++ LF+  +GI DP T  HYDNML AQIDA Y+AL
Sbjct: 194 KTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSAL 253

Query: 263 ADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRKRL--AKKKGTPFRPKNVL 322
           A AG++ + V ++ETGW S+GD++E  AT  NA+ +N NL K +   KK  TP +P N L
Sbjct: 254 AAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDL 313

Query: 323 KAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGF 357
             Y+FA FNENLKPGPTSERN+GLFK DGT +Y +GF
Sbjct: 314 SIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

BLAST of Lcy08g017430 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 315.5 bits (807), Expect = 9.1e-85
Identity = 146/321 (45.48%), Postives = 216/321 (67.29%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD  VL +F+ + ++ ++GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  MSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKL 157
           MS     A +W+++ ++  + +T I  I +GNE+    D  L  +LL A +++Y A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
            L+  V +T+AHS  + S S+PPSS  F+E  +QY++PLL+F S+I SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETG 277
              P+ + + Y LF+  +G+ DP T+LHYDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           W S+GDENE  A+  NA ++N NL K + ++KGTP +    +  Y+FA FNENLKPGP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 338 ERNFGLFKADGTISYDIGFHG 359
           ERN+GLF  DG   Y++G  G
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

BLAST of Lcy08g017430 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.0e-83
Identity = 159/352 (45.17%), Postives = 226/352 (64.20%), Query Frame = 0

Query: 19  VFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKA 78
           +FS       ++     + GINYG++A+N+P P  V+ LLK+     V++YDAD + L+A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 79  FNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFE 138
           F G+G EL V L N +L +MS     A  WVKENVQA+LP T I  I +GNEVL      
Sbjct: 69  FAGSGFELTVALGNEYLAQMS-DPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 128

Query: 139 LWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLE 198
           L  AL  A ++I+ A+    L+  + +TTAHS A+   S+PPS+  FR +++  + P+L+
Sbjct: 129 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 188

Query: 199 FFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAY 258
           F  + GSP  +NAYPF AY  +P+++ +++ LF+  +G  DP ++ HYDNML AQ+DA Y
Sbjct: 189 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 248

Query: 259 AALADAG--YQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRK-RLAKKKGTPFRP 318
            AL   G  Y+K+ ++V+ETGW S GD  E  AT +NAR +N NL K  ++KK  TP RP
Sbjct: 249 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 308

Query: 319 KNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLKSSSSDSS 368
           +  L  ++FA FNEN+KPGPTSERN+GLF  DGT  Y +   G+K+SS+ SS
Sbjct: 309 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSL---GIKTSSTHSS 356

BLAST of Lcy08g017430 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 3.0e-80
Identity = 150/336 (44.64%), Postives = 210/336 (62.50%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKE 97
           G+NYG++ADN+P P E V LL++  I+ VR+Y AD  ++KA  GTG+ +V+G  NG +  
Sbjct: 27  GVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPS 86

Query: 98  MSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKL 157
           +++    A  W+  NV  F P + I  I +GNE+L   D  L   LL A +N+ KA++ +
Sbjct: 87  LASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAV 146

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
           +L G ++++T +S  V  +S PPSS  F       +K +L+F S+ GSPF +N YPF AY
Sbjct: 147 SLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAY 206

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETG 277
            SDP    + + LFE   G  D KT + Y NM DAQ+DA ++AL   G++K+E++V ETG
Sbjct: 207 QSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETG 266

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           WASRGD NE  A+ +NA+ +N NL   L    GTP  P   +  YIFA ++ENLKPGP+S
Sbjct: 267 WASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSS 326

Query: 338 ERNFGLFKADGTISYDIGFHGLKSSSSDSSYLSLKV 374
           ER FGLFK D ++ YD+   GL  SSS S   S KV
Sbjct: 327 ERAFGLFKTDLSMVYDV---GLAKSSSSSQTPSGKV 359

BLAST of Lcy08g017430 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.7e-75
Identity = 148/343 (43.15%), Postives = 206/343 (60.06%), Query Frame = 0

Query: 16  FWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKV 75
           FW+  S +A L           GI YGR ADN+PSP+ V  L++   IK VRIYDA+  V
Sbjct: 9   FWIFVSILAFLNF---GMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDV 68

Query: 76  LKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGA 135
           LKAF  TG+EL++G+PN  L   +  + +   W+  N+  + P T I  I++G EV    
Sbjct: 69  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 128

Query: 136 DFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKP 195
           D    G +L A  NI+ A+KK  LD  ++I+++HS A+ S SFPPSS  F +    ++KP
Sbjct: 129 D-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKP 188

Query: 196 LLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQID 255
           +LEF  E  SPF ++ YP+ AY    E + + YALFES+  + DP T L Y NM DAQ+D
Sbjct: 189 MLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLD 248

Query: 256 AAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRP 315
           A Y AL    ++ ++V+VTE+GW S+G   E AAT  NA  +N NL + +    GTP +P
Sbjct: 249 AIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKP 308

Query: 316 KNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHG 359
              +  Y+F+ FNEN KPG  SERN+G+F A+GT  Y + F G
Sbjct: 309 GEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTG 347

BLAST of Lcy08g017430 vs. ExPASy TrEMBL
Match: A0A1S3C5G6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.4e-207
Identity = 370/411 (90.02%), Postives = 378/411 (91.97%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKC WFC QS+PGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAF+GTGLELVVGLPNGFL+EMSASEEHAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWGALLGAA+NIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           SSSSDSSYLSLK        D                SLTISAVTLLLLLR
Sbjct: 361 SSSSDSSYLSLK--------DFRARGL----SGSHMISLTISAVTLLLLLR 399

BLAST of Lcy08g017430 vs. ExPASy TrEMBL
Match: A0A0A0LF78 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.5e-204
Identity = 365/411 (88.81%), Postives = 376/411 (91.48%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKC WFC QS+ GFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSLSGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAF+GTGLELVVGLPNGFL+EMSASEEHAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWG LLGAA+NIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGTLLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
            SSSDSSYLSLK       +  H              SLTI+A+TLLLLLR
Sbjct: 361 YSSSDSSYLSLKDFRARGLLGSH------------MISLTITAMTLLLLLR 399

BLAST of Lcy08g017430 vs. ExPASy TrEMBL
Match: A0A6J1FIR4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444294 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 1.0e-203
Identity = 367/411 (89.29%), Postives = 376/411 (91.48%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QSVPGFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AF+GTGLELV+GLPNGFLKEMS+SEE AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWGALLGA +NIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           SSSS SSYLSLK            H A     +L  FSLT+S VT LLLLR
Sbjct: 361 SSSSSSSYLSLK-----------NHRARGLAGSL-IFSLTVSVVTRLLLLR 399

BLAST of Lcy08g017430 vs. ExPASy TrEMBL
Match: A0A6J1CPQ3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111013168 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.8e-203
Identity = 363/409 (88.75%), Postives = 378/409 (92.42%), Query Frame = 0

Query: 3   RKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAK 62
           R+C  FCVQS+P FWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAK
Sbjct: 5   RQCFCFCVQSLPSFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAK 64

Query: 63  IKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHI 122
           IKNVRIYDADH+VLKAF+GTGLELVVGLPNGFLKEMSASE+ AMNWVKENVQAFLPQTHI
Sbjct: 65  IKNVRIYDADHRVLKAFSGTGLELVVGLPNGFLKEMSASEDRAMNWVKENVQAFLPQTHI 124

Query: 123 CGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSS 182
           CGIAIGNEVLGG D ELWGALLGAA+NIYKAVKKLNLDG+VQITTAHSQAVFSNSFPPSS
Sbjct: 125 CGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKKLNLDGIVQITTAHSQAVFSNSFPPSS 184

Query: 183 CIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKT 242
           CIFRENVVQYMKPLL+FFSEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GI DPKT
Sbjct: 185 CIFRENVVQYMKPLLDFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTQGISDPKT 244

Query: 243 SLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLR 302
            LHYDNMLDAQIDAAYAAL DAGYQKMEVIVTETGWAS GDENEAAATANNARIFNYNLR
Sbjct: 245 GLHYDNMLDAQIDAAYAALVDAGYQKMEVIVTETGWASHGDENEAAATANNARIFNYNLR 304

Query: 303 KRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLKSS 362
           KRLAKKKGTPFRPKNVLKAYIFATFNEN+KPGPTSERNFGLFKADGTISYDIGFHGLKSS
Sbjct: 305 KRLAKKKGTPFRPKNVLKAYIFATFNENMKPGPTSERNFGLFKADGTISYDIGFHGLKSS 364

Query: 363 SSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           S+DSSYLSL+ +    F+              R FSLTI A+TLLLLLR
Sbjct: 365 SADSSYLSLQDLQARGFL------------VNRIFSLTIPAMTLLLLLR 401

BLAST of Lcy08g017430 vs. ExPASy TrEMBL
Match: A0A6J1HML7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465570 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.5e-202
Identity = 365/411 (88.81%), Postives = 375/411 (91.24%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QSVPGFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AF+GTGLELVVGLPNGFLKEMS+SEE AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWGALLGA +NIYKAVKKLNLDGV+QITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEI SPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           SSSS SSYLSLK            H A     +L  FSLT+S +T LLLLR
Sbjct: 361 SSSSSSSYLSLK-----------NHRARGLAGSL-IFSLTVSVMTRLLLLR 399

BLAST of Lcy08g017430 vs. NCBI nr
Match: XP_008457422.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo])

HSP 1 Score: 730.3 bits (1884), Expect = 9.0e-207
Identity = 370/411 (90.02%), Postives = 378/411 (91.97%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKC WFC QS+PGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAF+GTGLELVVGLPNGFL+EMSASEEHAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWGALLGAA+NIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           SSSSDSSYLSLK        D                SLTISAVTLLLLLR
Sbjct: 361 SSSSDSSYLSLK--------DFRARGL----SGSHMISLTISAVTLLLLLR 399

BLAST of Lcy08g017430 vs. NCBI nr
Match: XP_038889136.1 (glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida])

HSP 1 Score: 723.4 bits (1866), Expect = 1.1e-204
Identity = 366/411 (89.05%), Postives = 377/411 (91.73%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKC WFC QS+PGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVV LLKA
Sbjct: 1   MARKCFWFCFQSLPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVLLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAF+GTGLELV+GLPNGFL+EMSASEEHAMNWVKENVQAFLP T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVIGLPNGFLREMSASEEHAMNWVKENVQAFLPDT 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG+D ELWGALLGAA+NIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGSDLELWGALLGAAKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLL+F SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLQFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWAS GDENEAAATANNARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASHGDENEAAATANNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLF+ADG+ISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFQADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           + SSDSSYLSLK          H             FS  ISAVTLLLLLR
Sbjct: 361 APSSDSSYLSLKNFRARGLWANH------------VFSFMISAVTLLLLLR 399

BLAST of Lcy08g017430 vs. NCBI nr
Match: XP_004139302.2 (glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN60690.1 hypothetical protein Csa_019301 [Cucumis sativus])

HSP 1 Score: 720.7 bits (1859), Expect = 7.2e-204
Identity = 365/411 (88.81%), Postives = 376/411 (91.48%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MARKC WFC QS+ GFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSLSGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLKAF+GTGLELVVGLPNGFL+EMSASEEHAMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWG LLGAA+NIYKAVK LNLDGVVQITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGTLLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEIGSPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT LHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATA+NARIFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGLK
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
            SSSDSSYLSLK       +  H              SLTI+A+TLLLLLR
Sbjct: 361 YSSSDSSYLSLKDFRARGLLGSH------------MISLTITAMTLLLLLR 399

BLAST of Lcy08g017430 vs. NCBI nr
Match: XP_022938105.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata])

HSP 1 Score: 719.2 bits (1855), Expect = 2.1e-203
Identity = 367/411 (89.29%), Postives = 376/411 (91.48%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QSVPGFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AF+GTGLELV+GLPNGFLKEMS+SEE AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWGALLGA +NIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           SSSS SSYLSLK            H A     +L  FSLT+S VT LLLLR
Sbjct: 361 SSSSSSSYLSLK-----------NHRARGLAGSL-IFSLTVSVVTRLLLLR 399

BLAST of Lcy08g017430 vs. NCBI nr
Match: KAG6586386.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 717.6 bits (1851), Expect = 6.1e-203
Identity = 366/411 (89.05%), Postives = 376/411 (91.48%), Query Frame = 0

Query: 1   MARKCLWFCVQSVPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QSVPGFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AF+GTGLELV+GLPNGFLKEMS+SEE AMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGG D ELWGALLGA +NIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYM+PLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMEPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYN 300
           KT+L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATANNA+IFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLK 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSDSSYLSLKVVLKTIFIDIHTHSACLCEQNLRAFSLTISAVTLLLLLR 412
           SSSS SSYLSLK            H A     +L  FSLT+S VT LLLLR
Sbjct: 361 SSSSSSSYLSLK-----------NHRARGLAGSL-IFSLTVSVVTRLLLLR 399

BLAST of Lcy08g017430 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 539.3 bits (1388), Expect = 2.8e-153
Identity = 265/358 (74.02%), Postives = 305/358 (85.20%), Query Frame = 0

Query: 16  FWVVFSAIASLTMTVQ-AFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHK 75
           F+  F  + SL   V  AF GTYGINYGRIADNIPSP++VV LLK AKI+NVRIYD DH 
Sbjct: 12  FFFFFFNLTSLEYQVDGAFVGTYGINYGRIADNIPSPEKVVLLLKQAKIRNVRIYDVDHT 71

Query: 76  VLKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGG 135
           VL+AF+GTGL+LVVGLPNGFLKEMS++ +HA +WVKEN+Q+FLP+T I GIAIGNEVLGG
Sbjct: 72  VLEAFSGTGLDLVVGLPNGFLKEMSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGG 131

Query: 136 ADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMK 195
            D EL GALLGAA+N+Y A+KK+NL+  VQITTAHSQAVFS+S+PPSSC+F+ENVVQ+MK
Sbjct: 132 GDSELAGALLGAAKNVYNALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMK 191

Query: 196 PLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQI 255
           PLLEFF +IGSPFCLNAYPFLAY  +P+ IDINYALF+ TEGI+DPKT LHYDNMLDAQI
Sbjct: 192 PLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQI 251

Query: 256 DAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFR 315
           DAAY AL DAG++KMEV++TETGWAS+GD +E AAT  NAR +NYNLRKRLAKKKGTP R
Sbjct: 252 DAAYMALQDAGFKKMEVMITETGWASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLR 311

Query: 316 PKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLKSSSSDSSYLSLK 373
           PK VLKAYIFA FNEN KPG +SE +FGLFK DGTISYDIGF+ LKS S  S   S K
Sbjct: 312 PKTVLKAYIFALFNENSKPGKSSETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSK 369

BLAST of Lcy08g017430 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 473.8 bits (1218), Expect = 1.4e-133
Identity = 228/350 (65.14%), Postives = 280/350 (80.00%), Query Frame = 0

Query: 16  FWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKV 75
           F +VFS ++S   T  AF GTYG+NYGRIADN+PSPD V +LLK+AKI+N RIYDADH V
Sbjct: 24  FLLVFSILSS--QTAVAFIGTYGVNYGRIADNLPSPDAVATLLKSAKIRNTRIYDADHSV 83

Query: 76  LKAFNGTGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFL-PQTHICGIAIGNEVLGG 135
           L AF GTG+E++VGL N FLK++S  E+ AMNW+KENV+ F+   T I GIA+GNE+LGG
Sbjct: 84  LTAFRGTGIEIIVGLGNEFLKDISVGEDRAMNWIKENVEPFIRGGTKISGIAVGNEILGG 143

Query: 136 ADFELWGALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMK 195
            D  LW ALL AA+N+Y A+++L L  VV++++ HS+AVF+NS+PPSSC FR++V  +MK
Sbjct: 144 TDIGLWEALLPAAKNVYSALRRLGLHNVVEVSSPHSEAVFANSYPPSSCTFRDDVAPFMK 203

Query: 196 PLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQI 255
           PLL FF +I SPF +NAYPFLAY SDP  IDINYALFE  +GI DPKT LHYDNM DA +
Sbjct: 204 PLLAFFWQIQSPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDNMFDAMV 263

Query: 256 DAAYAALADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFR 315
           DA+YAAL  AGY K+ VIV+ETGWAS+GD +E  A+  NAR +N NLRKRL K+KGTP+R
Sbjct: 264 DASYAALEKAGYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKRKGTPYR 323

Query: 316 PKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLKSSSS 365
           P  V++AY+FA FNEN KPGPTSERNFGLFK DGTI+YDIG  GLKSSS+
Sbjct: 324 PDMVVRAYVFALFNENSKPGPTSERNFGLFKPDGTIAYDIGLTGLKSSSA 371

BLAST of Lcy08g017430 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 5.8e-119
Identity = 205/275 (74.55%), Postives = 237/275 (86.18%), Query Frame = 0

Query: 98  MSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKL 157
           MS++ +HA +WVKEN+Q+FLP+T I GIAIGNEVLGG D EL GALLGAA+N+Y A+KK+
Sbjct: 1   MSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVYNALKKM 60

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
           NL+  VQITTAHSQAVFS+S+PPSSC+F+ENVVQ+MKPLLEFF +IGSPFCLNAYPFLAY
Sbjct: 61  NLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAY 120

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETG 277
             +P+ IDINYALF+ TEGI+DPKT LHYDNMLDAQIDAAY AL DAG++KMEV++TETG
Sbjct: 121 TYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEVMITETG 180

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           WAS+GD +E AAT  NAR +NYNLRKRLAKKKGTP RPK VLKAYIFA FNEN KPG +S
Sbjct: 181 WASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENSKPGKSS 240

Query: 338 ERNFGLFKADGTISYDIGFHGLKSSSSDSSYLSLK 373
           E +FGLFK DGTISYDIGF+ LKS S  S   S K
Sbjct: 241 ETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSK 275

BLAST of Lcy08g017430 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 327.0 bits (837), Expect = 2.1e-89
Identity = 158/337 (46.88%), Postives = 227/337 (67.36%), Query Frame = 0

Query: 23  IASLTMTVQAFTGT-YGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFNG 82
           + SLT+ +   T T  G+NYG+I DN+PSP +V+ L+K+     V++YDA+ ++LKAF+ 
Sbjct: 14  LISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSN 73

Query: 83  TGLELVVGLPNGFLKEMSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWG 142
           TG+E ++GL N +L +M      A+ W+K+NV  FLP T+I  I IGNE+L   D  L  
Sbjct: 74  TGIEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTT 133

Query: 143 ALLGAAENIYKAVKKLNLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFS 202
            LL A + ++ A+    L   + +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  
Sbjct: 134 NLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHR 193

Query: 203 EIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAAL 262
           +  SPF +NAYPF AY  +P+ + +++ LF+  +GI DP T  HYDNML AQIDA Y+AL
Sbjct: 194 KTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSAL 253

Query: 263 ADAGYQKMEVIVTETGWASRGDENEAAATANNARIFNYNLRKRL--AKKKGTPFRPKNVL 322
           A AG++ + V ++ETGW S+GD++E  AT  NA+ +N NL K +   KK  TP +P N L
Sbjct: 254 AAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDL 313

Query: 323 KAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGF 357
             Y+FA FNENLKPGPTSERN+GLFK DGT +Y +GF
Sbjct: 314 SIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

BLAST of Lcy08g017430 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 315.5 bits (807), Expect = 6.5e-86
Identity = 146/321 (45.48%), Postives = 216/321 (67.29%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLKAFNGTGLELVVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD  VL +F+ + ++ ++GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  MSASEEHAMNWVKENVQAFLPQTHICGIAIGNEVLGGADFELWGALLGAAENIYKAVKKL 157
           MS     A +W+++ ++  + +T I  I +GNE+    D  L  +LL A +++Y A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVVQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAY 217
            L+  V +T+AHS  + S S+PPSS  F+E  +QY++PLL+F S+I SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTEGIFDPKTSLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETG 277
              P+ + + Y LF+  +G+ DP T+LHYDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAATANNARIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           W S+GDENE  A+  NA ++N NL K + ++KGTP +    +  Y+FA FNENLKPGP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 338 ERNFGLFKADGTISYDIGFHG 359
           ERN+GLF  DG   Y++G  G
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8683.0e-8846.88Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Q9ZQG99.1e-8545.48Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500... [more]
Q9FHX55.0e-8345.17Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100... [more]
Q9M0693.0e-8044.64Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE51.7e-7543.15Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
A0A1S3C5G64.4e-20790.02(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV... [more]
A0A0A0LF783.5e-20488.81(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3... [more]
A0A6J1FIR41.0e-20389.29(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444294 P... [more]
A0A6J1CPQ33.8e-20388.75(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111013168 ... [more]
A0A6J1HML72.5e-20288.81(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465570 PE=... [more]
Match NameE-valueIdentityDescription
XP_008457422.19.0e-20790.02PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo][more]
XP_038889136.11.1e-20489.05glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida][more]
XP_004139302.27.2e-20488.81glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN60690.1 hypothetical p... [more]
XP_022938105.12.1e-20389.29glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata][more]
KAG6586386.16.1e-20389.05Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
AT2G26600.12.8e-15374.02Glycosyl hydrolase superfamily protein [more]
AT3G15800.11.4e-13365.14Glycosyl hydrolase superfamily protein [more]
AT2G26600.25.8e-11974.55Glycosyl hydrolase superfamily protein [more]
AT1G32860.12.1e-8946.88Glycosyl hydrolase superfamily protein [more]
AT2G27500.16.5e-8645.48Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 38..356
e-value: 8.4E-79
score: 265.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 36..362
e-value: 9.3E-115
score: 384.9
NoneNo IPR availablePANTHERPTHR32227:SF129GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 27..383
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 27..383
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 37..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g017430.1Lcy08g017430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds