Lcy08g014250 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g014250
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptiontranscription factor bHLH149-like
LocationChr08: 44453777 .. 44454364 (-)
RNA-Seq ExpressionLcy08g014250
SyntenyLcy08g014250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCTCCGATTTCAGAAGCGGAGGAATCGAAGAACTCGAGAAGGTCGTTGCAGCAGCCGAATAAACGCCGGAAGATTGCTTCCGATGTTTGTGTGGATGGCAGAGAACGGAAGTGGAGGACAGAGGTCGAGCAACAGATCTACTCTTCAAAGCTCGCGGAAGCGCTCCGGAGAGACAGCCGTCGGCGGTCCTCGACGCGGCTAGTTCGCGAAACCGCATATGGCGTCCTCGCCGCCGCGGCCAAGGGGAGGACACGATGGAGTCGCGCGATTCTGGCGAGTCGATTCCGCCAGAGGCTCGCGAGGCGTCGGAGGACGAAGAAATTGACCGGCCGTAAACCGTCGCGCGCGGCGGAGGCGAAGGCGATGGCCGAGAAAAGTAGGAAGTTTCCGGCGGTGCAGAGGAAGGCGAAAATTCTCGGCCGGTTGATTCCAGGCTGCCGGAAAGTCTCTTTCTCGAACCTTCTGGAAGAAACGACCGATTACATACCGGCTTTGGAGATGCAGGTAAGAGCCATGGCGGCGTTGGTCGAACTCCTCGCCGGAGCTCAAAGTAATTTGCTCGGAATCTCGAACGATTCTTGA

mRNA sequence

ATGGACGCTCCGATTTCAGAAGCGGAGGAATCGAAGAACTCGAGAAGGTCGTTGCAGCAGCCGAATAAACGCCGGAAGATTGCTTCCGATGTTTGTGTGGATGGCAGAGAACGGAAGTGGAGGACAGAGGTCGAGCAACAGATCTACTCTTCAAAGCTCGCGGAAGCGCTCCGGAGAGACAGCCGTCGGCGGTCCTCGACGCGGCTAGTTCGCGAAACCGCATATGGCGTCCTCGCCGCCGCGGCCAAGGGGAGGACACGATGGAGTCGCGCGATTCTGGCGAGTCGATTCCGCCAGAGGCTCGCGAGGCGTCGGAGGACGAAGAAATTGACCGGCCGTAAACCGTCGCGCGCGGCGGAGGCGAAGGCGATGGCCGAGAAAAGTAGGAAGTTTCCGGCGGTGCAGAGGAAGGCGAAAATTCTCGGCCGGTTGATTCCAGGCTGCCGGAAAGTCTCTTTCTCGAACCTTCTGGAAGAAACGACCGATTACATACCGGCTTTGGAGATGCAGGTAAGAGCCATGGCGGCGTTGGTCGAACTCCTCGCCGGAGCTCAAAGTAATTTGCTCGGAATCTCGAACGATTCTTGA

Coding sequence (CDS)

ATGGACGCTCCGATTTCAGAAGCGGAGGAATCGAAGAACTCGAGAAGGTCGTTGCAGCAGCCGAATAAACGCCGGAAGATTGCTTCCGATGTTTGTGTGGATGGCAGAGAACGGAAGTGGAGGACAGAGGTCGAGCAACAGATCTACTCTTCAAAGCTCGCGGAAGCGCTCCGGAGAGACAGCCGTCGGCGGTCCTCGACGCGGCTAGTTCGCGAAACCGCATATGGCGTCCTCGCCGCCGCGGCCAAGGGGAGGACACGATGGAGTCGCGCGATTCTGGCGAGTCGATTCCGCCAGAGGCTCGCGAGGCGTCGGAGGACGAAGAAATTGACCGGCCGTAAACCGTCGCGCGCGGCGGAGGCGAAGGCGATGGCCGAGAAAAGTAGGAAGTTTCCGGCGGTGCAGAGGAAGGCGAAAATTCTCGGCCGGTTGATTCCAGGCTGCCGGAAAGTCTCTTTCTCGAACCTTCTGGAAGAAACGACCGATTACATACCGGCTTTGGAGATGCAGGTAAGAGCCATGGCGGCGTTGGTCGAACTCCTCGCCGGAGCTCAAAGTAATTTGCTCGGAATCTCGAACGATTCTTGA

Protein sequence

MDAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDSRRRSSTRLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALVELLAGAQSNLLGISNDS
Homology
BLAST of Lcy08g014250 vs. ExPASy Swiss-Prot
Match: O80482 (Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 91/161 (56.52%), Postives = 109/161 (67.70%), Query Frame = 0

Query: 38  RKWRTEVEQQIYSSKLAEALRRDSRRRSST-------------RLVRETAYGVLAAAAKG 97
           ++W+T   QQIY+ KL EALRR  +R S+T             R +R+TA  VLAA+A+G
Sbjct: 42  KRWKTNRVQQIYACKLVEALRRVRQRSSTTSNNETDKLVSGAAREIRDTADRVLAASARG 101

Query: 98  RTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAEAKAMAEKSR-KFPAVQRKAKILGR 157
            TRWSRAILASR R +L + R+ KK TG   SR    K + E +R K PAV+RK KILGR
Sbjct: 102 TTRWSRAILASRVRAKLKKHRKAKKSTGNCKSR----KGLTETNRIKLPAVERKLKILGR 161

Query: 158 LIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALVELLAGA 185
           L+PGCRKVS  NLL+E TDYI ALEMQVRAM AL ELL  A
Sbjct: 162 LVPGCRKVSVPNLLDEATDYIAALEMQVRAMEALAELLTAA 198

BLAST of Lcy08g014250 vs. ExPASy Swiss-Prot
Match: Q9M9L6 (Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.3e-24
Identity = 77/169 (45.56%), Postives = 105/169 (62.13%), Query Frame = 0

Query: 18  LQQPNKRRKIASDVC-VDGRERKWRTEVEQQIYSSKLAEALRRDSRRRSSTRLVRETAYG 77
           L +  +R +++S+   V    R+WR    Q++YS KL +AL+R   RRS+T  VR+TA  
Sbjct: 40  LMEALRRSRVSSEEAPVRHLSRRWRATTAQKVYSLKLYDALQRS--RRSAT--VRDTADK 99

Query: 78  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAEAKAMAEKSRKFPAVQR 137
           VLA  A+G TRWSRAIL SRF   L RRR TK  +    + A      + + RK  AV  
Sbjct: 100 VLATTARGATRWSRAILVSRFGTSLRRRRNTKPASAL--AAAIRGSGGSGRRRKLSAVGN 159

Query: 138 KAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALVELLAGAQ 186
           + ++LG L+PGCR+ +   LL+ET DYI ALEMQVRAM AL ++L+  Q
Sbjct: 160 RVRVLGGLVPGCRRTALPELLDETADYIAALEMQVRAMTALSKILSELQ 202

BLAST of Lcy08g014250 vs. ExPASy Swiss-Prot
Match: Q9LSN7 (Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-23
Identity = 84/217 (38.71%), Postives = 117/217 (53.92%), Query Frame = 0

Query: 4   PISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALR--RDS 63
           P + +  S  SRR  ++ +    +           KWR+E +QQIYS+KL  ALR  R S
Sbjct: 14  PTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHALRELRIS 73

Query: 64  RRRSST---------RLVRETAYGVLAAAAKGRTRWSRAILASRF--------RQRLARR 123
           ++ SS+         R VRE A   LA AA+G+T WSRAIL+           RQR++  
Sbjct: 74  QQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKRQRISNP 133

Query: 124 RRTKKLTGRKPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYI 183
             T   TG   S+   A  +  K++  PAVQRK K+L RL+PGCRK S   +LEETTDYI
Sbjct: 134 TTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLEETTDYI 193

Query: 184 PALEMQVRAMAALVELLA-----------GAQSNLLG 191
            A+EMQ+R M A++  ++           G Q+++LG
Sbjct: 194 AAMEMQIRTMTAILSAVSSSPPPPTPGHEGGQTHMLG 230

BLAST of Lcy08g014250 vs. ExPASy Swiss-Prot
Match: Q9C8Z9 (Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.2e-20
Identity = 79/194 (40.72%), Postives = 109/194 (56.19%), Query Frame = 0

Query: 12  KNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRR----DSRRRSST 71
           +  R S       R  AS V  +   R WR+E +Q+IYS+KL +AL++     S   SS+
Sbjct: 21  RKKRSSASSAASSRSSASSVSGEIHAR-WRSEKQQRIYSAKLFQALQQVRLNSSASTSSS 80

Query: 72  -------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAE 131
                  + VRE A   LA +A+GRT WSRAILA+R + +  R++R  + T   P+    
Sbjct: 81  PTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKF-RKQRRPRATMAIPAMTTV 140

Query: 132 AKAMAEKSRK------------FPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALE 183
             + + +SRK             P V RK ++LGRL+PGC K S   +LEE TDYI ALE
Sbjct: 141 VSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQALE 200

BLAST of Lcy08g014250 vs. ExPASy Swiss-Prot
Match: Q7XR02 (Transcription factor IBH1 OS=Oryza sativa subsp. japonica OX=39947 GN=IBH1 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 8.9e-06
Identity = 48/136 (35.29%), Postives = 67/136 (49.26%), Query Frame = 0

Query: 67  TRLVRETAYGVLAAAAKGRTRWSRAIL----ASRFRQRLAR-----RRRTKKLTGRKPSR 126
           TR +R  AY  +A AA  R  WSRA+L    A R R  + R     RRR        P+ 
Sbjct: 65  TRTIRRAAYSSMARAASPRRAWSRALLGQVRARRSRTLMRRAAVLVRRRVVAAPAPSPAS 124

Query: 127 AAEAKAMA-----EKSRKFPAVQR------KAKILGRLIPGCRKVSFSNLLEETTDYIPA 183
           A   + +A       +R  P   R      +A+ L RL+PG   + +S+LLEET DY+ +
Sbjct: 125 ARGVRIIAAGETSAAARAVPPPPRQQGEPPRAEALRRLVPGGAGMEYSSLLEETADYLRS 184

BLAST of Lcy08g014250 vs. ExPASy TrEMBL
Match: A0A6J1FBF1 (transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111444091 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 6.3e-71
Identity = 161/194 (82.99%), Postives = 168/194 (86.60%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+A ESKNSRR LQQPNKRRKIASDVC +GRERKWRTEVEQQIYSSKL EA+R  S
Sbjct: 3   DAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K PAVQRK KILGRL+PGCRKVSF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRMAETK--SEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. ExPASy TrEMBL
Match: A0A6J1HQH2 (transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111465681 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 7.7e-69
Identity = 159/194 (81.96%), Postives = 167/194 (86.08%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+AEESKNSRR LQ+PNKRRKIA DV   GRERKWRTEVEQQIYSSKL EA+R+ S
Sbjct: 3   DAPISDAEESKNSRRLLQKPNKRRKIAFDVGAKGRERKWRTEVEQQIYSSKLVEAIRQVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K PAVQRK KILGRL+PGCRKVSF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRIAETK--SEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. ExPASy TrEMBL
Match: A0A6J1KCR3 (transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111493732 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.6e-64
Identity = 150/197 (76.14%), Postives = 165/197 (83.76%), Query Frame = 0

Query: 7   EAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDSRRRSS 66
           +AEESKNSRRSLQ+PNKR +IAS+V  +GRER+WRTEVEQQIYSS+LAEAL+R SR+RSS
Sbjct: 2   DAEESKNSRRSLQKPNKRLQIASNVSAEGRERRWRTEVEQQIYSSRLAEALQRVSRQRSS 61

Query: 67  T--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRA 126
                    RLVRETA  VLAAAAKGRTRWSRAILA+RFRQRLARRRRTKK  GRKPSR 
Sbjct: 62  PHARSTINGRLVRETADCVLAAAAKGRTRWSRAILANRFRQRLARRRRTKKSIGRKPSRV 121

Query: 127 AEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALV 186
            E      K+RK PAVQRK KILGRL+PGCRK+SF NLLEE TDYI ALEMQV+AMAAL 
Sbjct: 122 EEV-----KNRKLPAVQRKVKILGRLVPGCRKLSFPNLLEEATDYISALEMQVKAMAALA 181

Query: 187 ELLAGAQSNLLGISNDS 196
           E+LAGAQSNLL ISNDS
Sbjct: 182 EILAGAQSNLLPISNDS 193

BLAST of Lcy08g014250 vs. ExPASy TrEMBL
Match: A0A6J1G430 (transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111450552 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.2e-63
Identity = 148/197 (75.13%), Postives = 163/197 (82.74%), Query Frame = 0

Query: 7   EAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDSRRRSS 66
           +AEESKNS+RSLQ+PNKRR+IASDV  +GRER+WRTEVEQQIYSS+LAEAL+R  R  SS
Sbjct: 2   DAEESKNSKRSLQKPNKRRQIASDVSAEGRERRWRTEVEQQIYSSRLAEALQRVGRLPSS 61

Query: 67  T--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRA 126
                    RLVRETA  VLAAAAKGRTRWSRAILA+RFRQRLARRRRTKK  GRKPSR 
Sbjct: 62  PHARSTINGRLVRETADCVLAAAAKGRTRWSRAILANRFRQRLARRRRTKKSIGRKPSRV 121

Query: 127 AEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALV 186
           AE      K+RK PAVQRK +ILGRL+PGCRK+SF NLLEE TDYI ALEMQV+AMAAL 
Sbjct: 122 AEV-----KNRKLPAVQRKVRILGRLVPGCRKLSFPNLLEEATDYISALEMQVKAMAALA 181

Query: 187 ELLAGAQSNLLGISNDS 196
           E+LAGAQ NLL ISNDS
Sbjct: 182 EILAGAQRNLLPISNDS 193

BLAST of Lcy08g014250 vs. ExPASy TrEMBL
Match: A0A5A7TYM9 (Transcription factor bHLH149-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001010 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.6e-45
Identity = 122/196 (62.24%), Postives = 144/196 (73.47%), Query Frame = 0

Query: 7   EAEESKNSRRSLQQPNKRR--KIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDSRRR 66
           +A++S NS   L  P  +R  KI +D   +  +  W+TE++QQIYSS+LAEALRR +  R
Sbjct: 2   DADQSNNSTTLLPHPANKRPPKIITD---EPTQHTWKTEIQQQIYSSRLAEALRRVTHPR 61

Query: 67  SST----RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLA-RRRRTKKLTGRKPSRAA 126
           SS+     LVR+TA  VLAA AKGR+RWSRAILA+RFRQ LA RRRRTKKL  RKP    
Sbjct: 62  SSSTINGNLVRQTADSVLAATAKGRSRWSRAILATRFRQSLARRRRRTKKLLARKP---- 121

Query: 127 EAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALVE 186
             +   EK+RK PAVQRK KILGRL+PGCRK+SF NLLEE TDYI ALEMQV+AM AL E
Sbjct: 122 --ELKNEKARKLPAVQRKVKILGRLVPGCRKLSFPNLLEEATDYISALEMQVKAMTALAE 181

Query: 187 LLAGAQSNLLGISNDS 196
           LLAG Q N+ GISNDS
Sbjct: 182 LLAGNQRNIAGISNDS 188

BLAST of Lcy08g014250 vs. NCBI nr
Match: KAG7021037.1 (Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 277.3 bits (708), Expect = 9.9e-71
Identity = 162/194 (83.51%), Postives = 168/194 (86.60%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+A ESKNSRR LQQPNKRRKIASDVC +GRERKWRTEVEQQIYSSKL EA+R  S
Sbjct: 3   DAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K PAVQRK KILGRLIPGCRKVSF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRMAETK--SEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. NCBI nr
Match: XP_022937811.1 (transcription factor bHLH149-like [Cucurbita moschata])

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-70
Identity = 161/194 (82.99%), Postives = 168/194 (86.60%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+A ESKNSRR LQQPNKRRKIASDVC +GRERKWRTEVEQQIYSSKL EA+R  S
Sbjct: 3   DAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K PAVQRK KILGRL+PGCRKVSF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRMAETK--SEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. NCBI nr
Match: XP_023537600.1 (transcription factor bHLH149-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-70
Identity = 160/194 (82.47%), Postives = 169/194 (87.11%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+AEESKNSRR LQQPNKRRKIASDVC +GRERKWRTEVEQQIYSSKL EA+R+ S
Sbjct: 3   DAPISDAEESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRQVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAA AKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAVAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K PAVQRK KILGRL+PGCRK+SF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRMAETK--SEKNKKLPAVQRKVKILGRLVPGCRKLSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. NCBI nr
Match: KAG6586210.1 (Transcription factor basic helix-loop-helix 149, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 275.8 bits (704), Expect = 2.9e-70
Identity = 160/194 (82.47%), Postives = 168/194 (86.60%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+A ESKNSRR LQQPNKRRKIASDVC +GRERKWRTEVEQQIYSSKL EA+R  S
Sbjct: 3   DAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K P+VQRK KILGRL+PGCRKVSF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRMAETK--SEKNKKLPSVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. NCBI nr
Match: XP_022965958.1 (transcription factor bHLH149-like [Cucurbita maxima])

HSP 1 Score: 270.0 bits (689), Expect = 1.6e-68
Identity = 159/194 (81.96%), Postives = 167/194 (86.08%), Query Frame = 0

Query: 2   DAPISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRRDS 61
           DAPIS+AEESKNSRR LQ+PNKRRKIA DV   GRERKWRTEVEQQIYSSKL EA+R+ S
Sbjct: 3   DAPISDAEESKNSRRLLQKPNKRRKIAFDVGAKGRERKWRTEVEQQIYSSKLVEAIRQVS 62

Query: 62  RRRSST--------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGR 121
           RRRSS         RLVRETA  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL GR
Sbjct: 63  RRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGR 122

Query: 122 KPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRA 181
           KPSR AE K  +EK++K PAVQRK KILGRL+PGCRKVSF NLLEETTDYI ALEMQVRA
Sbjct: 123 KPSRIAETK--SEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 182

Query: 182 MAALVELLAGAQSN 188
           MAALVELLAGAQSN
Sbjct: 183 MAALVELLAGAQSN 194

BLAST of Lcy08g014250 vs. TAIR 10
Match: AT1G09250.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 7.9e-34
Identity = 91/161 (56.52%), Postives = 109/161 (67.70%), Query Frame = 0

Query: 38  RKWRTEVEQQIYSSKLAEALRRDSRRRSST-------------RLVRETAYGVLAAAAKG 97
           ++W+T   QQIY+ KL EALRR  +R S+T             R +R+TA  VLAA+A+G
Sbjct: 42  KRWKTNRVQQIYACKLVEALRRVRQRSSTTSNNETDKLVSGAAREIRDTADRVLAASARG 101

Query: 98  RTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAEAKAMAEKSR-KFPAVQRKAKILGR 157
            TRWSRAILASR R +L + R+ KK TG   SR    K + E +R K PAV+RK KILGR
Sbjct: 102 TTRWSRAILASRVRAKLKKHRKAKKSTGNCKSR----KGLTETNRIKLPAVERKLKILGR 161

Query: 158 LIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALVELLAGA 185
           L+PGCRKVS  NLL+E TDYI ALEMQVRAM AL ELL  A
Sbjct: 162 LVPGCRKVSVPNLLDEATDYIAALEMQVRAMEALAELLTAA 198

BLAST of Lcy08g014250 vs. TAIR 10
Match: AT3G05800.1 (AtBS1(activation-tagged BRI1 suppressor 1)-interacting factor 1 )

HSP 1 Score: 113.2 bits (282), Expect = 2.3e-25
Identity = 77/169 (45.56%), Postives = 105/169 (62.13%), Query Frame = 0

Query: 18  LQQPNKRRKIASDVC-VDGRERKWRTEVEQQIYSSKLAEALRRDSRRRSSTRLVRETAYG 77
           L +  +R +++S+   V    R+WR    Q++YS KL +AL+R   RRS+T  VR+TA  
Sbjct: 40  LMEALRRSRVSSEEAPVRHLSRRWRATTAQKVYSLKLYDALQRS--RRSAT--VRDTADK 99

Query: 78  VLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAEAKAMAEKSRKFPAVQR 137
           VLA  A+G TRWSRAIL SRF   L RRR TK  +    + A      + + RK  AV  
Sbjct: 100 VLATTARGATRWSRAILVSRFGTSLRRRRNTKPASAL--AAAIRGSGGSGRRRKLSAVGN 159

Query: 138 KAKILGRLIPGCRKVSFSNLLEETTDYIPALEMQVRAMAALVELLAGAQ 186
           + ++LG L+PGCR+ +   LL+ET DYI ALEMQVRAM AL ++L+  Q
Sbjct: 160 RVRVLGGLVPGCRRTALPELLDETADYIAALEMQVRAMTALSKILSELQ 202

BLAST of Lcy08g014250 vs. TAIR 10
Match: AT3G17100.1 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-24
Identity = 84/217 (38.71%), Postives = 117/217 (53.92%), Query Frame = 0

Query: 4   PISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALR--RDS 63
           P + +  S  SRR  ++ +    +           KWR+E +QQIYS+KL  ALR  R S
Sbjct: 14  PTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHALRELRIS 73

Query: 64  RRRSST---------RLVRETAYGVLAAAAKGRTRWSRAILASRF--------RQRLARR 123
           ++ SS+         R VRE A   LA AA+G+T WSRAIL+           RQR++  
Sbjct: 74  QQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKRQRISNP 133

Query: 124 RRTKKLTGRKPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYI 183
             T   TG   S+   A  +  K++  PAVQRK K+L RL+PGCRK S   +LEETTDYI
Sbjct: 134 TTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLEETTDYI 193

Query: 184 PALEMQVRAMAALVELLA-----------GAQSNLLG 191
            A+EMQ+R M A++  ++           G Q+++LG
Sbjct: 194 AAMEMQIRTMTAILSAVSSSPPPPTPGHEGGQTHMLG 230

BLAST of Lcy08g014250 vs. TAIR 10
Match: AT3G17100.2 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-24
Identity = 84/217 (38.71%), Postives = 117/217 (53.92%), Query Frame = 0

Query: 4   PISEAEESKNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALR--RDS 63
           P + +  S  SRR  ++ +    +           KWR+E +QQIYS+KL  ALR  R S
Sbjct: 14  PTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHALRELRIS 73

Query: 64  RRRSST---------RLVRETAYGVLAAAAKGRTRWSRAILASRF--------RQRLARR 123
           ++ SS+         R VRE A   LA AA+G+T WSRAIL+           RQR++  
Sbjct: 74  QQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKRQRISNP 133

Query: 124 RRTKKLTGRKPSRAAEAKAMAEKSRKFPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYI 183
             T   TG   S+   A  +  K++  PAVQRK K+L RL+PGCRK S   +LEETTDYI
Sbjct: 134 TTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLEETTDYI 193

Query: 184 PALEMQVRAMAALVELLA-----------GAQSNLLG 191
            A+EMQ+R M A++  ++           G Q+++LG
Sbjct: 194 AAMEMQIRTMTAILSAVSSSPPPPTPGHEGGQTHMLG 230

BLAST of Lcy08g014250 vs. TAIR 10
Match: AT3G06590.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-21
Identity = 79/194 (40.72%), Postives = 109/194 (56.19%), Query Frame = 0

Query: 12  KNSRRSLQQPNKRRKIASDVCVDGRERKWRTEVEQQIYSSKLAEALRR----DSRRRSST 71
           +  R S       R  AS V  +   R WR+E +Q+IYS+KL +AL++     S   SS+
Sbjct: 21  RKKRSSASSAASSRSSASSVSGEIHAR-WRSEKQQRIYSAKLFQALQQVRLNSSASTSSS 80

Query: 72  -------RLVRETAYGVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLTGRKPSRAAE 131
                  + VRE A   LA +A+GRT WSRAILA+R + +  R++R  + T   P+    
Sbjct: 81  PTAQKRGKAVREAADRALAVSARGRTLWSRAILANRIKLKF-RKQRRPRATMAIPAMTTV 140

Query: 132 AKAMAEKSRK------------FPAVQRKAKILGRLIPGCRKVSFSNLLEETTDYIPALE 183
             + + +SRK             P V RK ++LGRL+PGC K S   +LEE TDYI ALE
Sbjct: 141 VSSSSNRSRKRRVSVLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQALE 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O804821.1e-3256.52Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=... [more]
Q9M9L63.3e-2445.56Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=... [more]
Q9LSN71.6e-2338.71Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=... [more]
Q9C8Z92.2e-2040.72Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=... [more]
Q7XR028.9e-0635.29Transcription factor IBH1 OS=Oryza sativa subsp. japonica OX=39947 GN=IBH1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FBF16.3e-7182.99transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111444091 ... [more]
A0A6J1HQH27.7e-6981.96transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111465681 PE... [more]
A0A6J1KCR32.6e-6476.14transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111493732 PE... [more]
A0A6J1G4302.2e-6375.13transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111450552 ... [more]
A0A5A7TYM91.6e-4562.24Transcription factor bHLH149-like protein OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
KAG7021037.19.9e-7183.51Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022937811.11.3e-7082.99transcription factor bHLH149-like [Cucurbita moschata][more]
XP_023537600.11.3e-7082.47transcription factor bHLH149-like [Cucurbita pepo subsp. pepo][more]
KAG6586210.12.9e-7082.47Transcription factor basic helix-loop-helix 149, partial [Cucurbita argyrosperma... [more]
XP_022965958.11.6e-6881.96transcription factor bHLH149-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G09250.17.9e-3456.52basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G05800.12.3e-2545.56AtBS1(activation-tagged BRI1 suppressor 1)-interacting factor 1 [more]
AT3G17100.11.1e-2438.71sequence-specific DNA binding transcription factors [more]
AT3G17100.21.1e-2438.71sequence-specific DNA binding transcription factors [more]
AT3G06590.11.6e-2140.72basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 107..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR33124:SF69TRANSCRIPTION FACTOR BHLH149coord: 15..189
IPR044660Transcription factor IBH1-likePANTHERPTHR33124TRANSCRIPTION FACTOR IBH1-LIKE 1coord: 15..189
IPR044549Transcription factor IBH1-like, bHLH domainCDDcd11444bHLH_AtIBH1_likecoord: 132..178
e-value: 5.44627E-15
score: 64.566
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 134..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g014250.1Lcy08g014250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000976 transcription cis-regulatory region binding