Lcy08g013950 (gene) Sponge gourd (P93075) v1

BLAST of Lcy08g013950 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 4.2e-115
Identity = 235/550 (42.73%), Postives = 337/550 (61.27%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F  + FLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK------DCVRGRIAF 138
           GFL+D + +     L+   +E L L+++T+QA    L+P P C        + V G  A 
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 148

Query: 139 VFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTL 198
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K  +T+FNY +  +  GA V VT 
Sbjct: 149 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 208

Query: 199 IVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVI----VVAI 258
           +VW+  NK W WGF  ST++  +  +VF +G  FY+  +P  SP+  I +V+    +V+ 
Sbjct: 209 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSC 268

Query: 259 KNRRLALPETPSELY-EINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEAWKVCSV 318
            ++  +   T  E+  E  +K  + S+      TN L  L+K AI GK      W  C+V
Sbjct: 269 SSKTSSNHFTSREVQSEHEEKTPSQSL------TNSLTCLNK-AIEGKTH--HIWLECTV 328

Query: 319 TQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAPSIPVIP 378
            QVE+VKI+ +M PIF CTI++N CLAQL T+SV Q  T  M+R I +   P+ S+PV P
Sbjct: 329 QQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFP 388

Query: 379 LLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEVKRRHQA 438
           ++FM  L P Y+ + +PFARK+T    GIT LQR+GVGLVLS ++MAVA LVE+KR+  A
Sbjct: 389 VVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVA 448

Query: 439 ------TTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 498
                  +    P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W S
Sbjct: 449 REAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWAS 508

Query: 499 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 552
           L+LGYYLSS+ V +VN+VTK    S   W    + E LN N L+LFYW + +LS++NFLH
Sbjct: 509 LALGYYLSSVMVPIVNRVTKSAGQSP--W----LGEKLNRNRLDLFYWLMCVLSVVNFLH 560

BLAST of Lcy08g013950 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 9.7e-112
Identity = 226/566 (39.93%), Postives = 337/566 (59.54%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG  A  F+  +   E++ ++AN  +LV Y    +H     +AN +TNF G+ FLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK---DCVRGRIAFVFY 138
           GFLSD + +     L+   +E L L+++T+QA +  L+P P C     + V G  A + +
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMP-PSCDSPTCEEVSGSKAAMLF 146

Query: 139 TSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTLIVW 198
             LYL+A+G GG++G+L + GA+QFD+  PK  K  +T+FNY +  +  GA V VT +VW
Sbjct: 147 VGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVW 206

Query: 199 VAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVA-------- 258
           +  NK W WGF  ST+A  V  ++F  G  FYR  +P  SP+  I++V++ A        
Sbjct: 207 LEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG 266

Query: 259 -IKNRRLALPETPS------------ELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILG 318
              N   ++  +PS               E+      +++  +   TN L+ L+ AA   
Sbjct: 267 SSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA--- 326

Query: 319 KDREP-EAWKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSI 378
            D +P      C+V QVE+VKI+ +M PIF CTI++N CLAQL TFSV+Q  +  M+  I
Sbjct: 327 -DEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKI 386

Query: 379 GHLQFPAPSIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISM 438
           G L+ P  S+P+ P++F+  L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS ++M
Sbjct: 387 GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAM 446

Query: 439 AVAGLVEVKRRHQA------TTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP 498
           AVA LVE+KR+  A       +    P++  W++ QY   G AD+FTL GLLE+F+ EAP
Sbjct: 447 AVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAP 506

Query: 499 MGMRSLSTSFTWLSLSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLF 554
             MRSL+TS +W SL++GYYLSS+ V++VN +T   +     W+ G   + +N   L+ F
Sbjct: 507 SSMRSLATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYF 566

BLAST of Lcy08g013950 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 3.7e-111
Identity = 229/537 (42.64%), Postives = 327/537 (60.89%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG RA +F+  L  FE MG  A G +L+ Y +  +HF L  AAN +TNF G+ F+ +LLG
Sbjct: 43  GGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLG 102

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLP---KPDCPKDC--VRGRIAFV 138
           G+LSD ++    T ++FG +E+   ++++VQA+   L P    P   + C   +G  A +
Sbjct: 103 GYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMI 162

Query: 139 FYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTLI 198
           F+ +LYL+A+GSG V+  + A GADQF Q  PK++K L++YFN    +  +G  + +TL+
Sbjct: 163 FFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLL 222

Query: 199 VWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVAIKNRRL 258
           VWV  +     GF  S  A  +G I    G  ++R   P +S    I  VIV AI  R+L
Sbjct: 223 VWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKL 282

Query: 259 ALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKD--REPEAWKVCSVTQVE 318
           A P  P  L+  +         S + HT + RFLDKA I  +D   +   W++C+VTQVE
Sbjct: 283 ASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVE 342

Query: 319 EVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAPSIPVIPLLFM 378
           +VK +  + PIF  TI+ NT LAQLQTFSV+QG++ +  R       P  S+  IP + +
Sbjct: 343 QVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSS-MNTRLSNSFHIPPASLQAIPYIML 402

Query: 379 TFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEVKRRHQATTHP 438
            FL+PLY+   VPFARK+T H SGI  L R+G+GL LS  SM  A ++E KRR  ++   
Sbjct: 403 IFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRR-DSSVLD 462

Query: 439 EKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLSLSLGYYLSSI 498
            + +S+FW++ Q+ IFGI++MFT VGL+EFFYK++  GM S   + T+ S S G+Y SS+
Sbjct: 463 GRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSV 522

Query: 499 FVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLHYLYWASW 549
            V+VVNK+T     SK GW   L   DLN + L+LFYW LA+LS+LNFL YL+W+ W
Sbjct: 523 LVSVVNKITSTSVDSK-GW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Lcy08g013950 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.9e-107
Identity = 233/549 (42.44%), Postives = 328/549 (59.74%), Query Frame = 0

Query: 14  RDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFL 73
           R    GG RA +F+     FE M   A G +L+ Y    +HF L  +AN +TNF G+ FL
Sbjct: 35  RPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFL 94

Query: 74  LSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK-----DCV-- 133
           LSLLGGFLSD+Y+    T LVFGV+EI   ++++VQA+  +L P P+C        CV  
Sbjct: 95  LSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP-PECNMKSTTIHCVEA 154

Query: 134 RGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGA 193
            G  A   YT+L L+A+GSG ++  + + GA+QF +KD    + L+++FN    +  +G 
Sbjct: 155 NGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQ 214

Query: 194 AVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVV 253
            + +TL+VWV  +     GF  S    A G I    G  FYR   P  S    I QV V 
Sbjct: 215 LIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVA 274

Query: 254 AIKNRRLALPETPSELYEINDKCYTDSIHSK-IAHTNQLRFLDKAAILGKDREPEA-WKV 313
           AI  R+   P  P+ +++ +    TD +  K + H+N+ RFLDKA I  + +  E+ W++
Sbjct: 275 AITKRKQICPSNPNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRL 334

Query: 314 CSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQG---NTRIMDRSIGHLQFPAP 373
           C++ QV +VKI+  + PIF CTII NT LAQLQTFSV+QG   NT I        Q P  
Sbjct: 335 CTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPA 394

Query: 374 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 433
           S+  IP + + F +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  SM  A LVE 
Sbjct: 395 SLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEK 454

Query: 434 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 493
           KRR ++       +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S
Sbjct: 455 KRR-ESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCS 514

Query: 494 LSLGYYLSSIFVNVVNKVTKKI-TPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFL 550
            S G+YLSS+ V+ VN+VT    + +K GW   L   DLN + L+ FYW LA LS +NF 
Sbjct: 515 YSFGFYLSSVLVSTVNRVTSSNGSGTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFF 567

BLAST of Lcy08g013950 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.5e-99
Identity = 206/535 (38.50%), Postives = 324/535 (60.56%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG RA   +  ++  E++ F+ANG + V+YF+  +H+   TAAN +TNF G++FLL+L G
Sbjct: 23  GGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAANMVTNFMGTSFLLTLFG 82

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPKDCVRGRIAFVFYTSL 138
           GF++D+++   TT +VF  +E++ L+++T QA++  LLP+ D     ++  I F   T L
Sbjct: 83  GFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKLLPEKDKTPSTLQSAILF---TGL 142

Query: 139 YLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTLIVWVAV 198
           Y +AIG+GG++ +LP+ G DQ D+++P   + ++ +F+++  SI  G  + VT+++W+  
Sbjct: 143 YAMAIGTGGLKASLPSHGGDQIDRRNP---RLISRFFDWLYFSICSGCLLAVTVVLWIEE 202

Query: 199 NKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVAIKNRRLALPET 258
            K W W F  S    A    +F +G PFYR   P  SP+ +I  VI+ A +NR  +  + 
Sbjct: 203 KKGWIWSFNISVGILATALCIFTVGLPFYRFKRPNGSPLKKIAIVIISAARNRNKS--DL 262

Query: 259 PSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEAWKVCSVTQVEEVKIITR 318
             E+       Y ++ H      N+L+++DKA +          K  S T+VEE +    
Sbjct: 263 DEEMMRGLISIYKNNSH------NKLKWIDKATLN---------KNISETEVEETRTFLG 322

Query: 319 MFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGH-LQFPAPSIPVIPLLFMTFLIPL 378
           + PIF  TI+M+ C+AQL TFS +QG   +M++ + H  + P PS+  IPL+FM   IPL
Sbjct: 323 LLPIFGSTIVMSCCVAQLSTFSAQQG--MLMNKKLFHSFEIPVPSLTAIPLIFMLLSIPL 382

Query: 379 YEFVFVPFARKIT---HHPSGITQLQRVGVGLVLSAISMAVAGLVEVKRRHQATTHPEKP 438
           YEF    F +KI+   ++ S    L+R+G+GL LS++SMAV+ +VE KR+H+   H    
Sbjct: 383 YEF----FGKKISSGNNNRSSSFNLKRIGLGLALSSVSMAVSAIVEAKRKHE-VVHNNFR 442

Query: 439 MSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLSLSLGYYLSSIFVN 498
           +S+ WL FQY +  ++DM TL G+LEFFY+EAP  M+S+ST+  W S +LG++LS+  V 
Sbjct: 443 ISVLWLVFQYLMLSVSDMLTLGGMLEFFYREAPSNMKSISTALGWCSTALGFFLSTTLVE 502

Query: 499 VVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLHYLYWASWY 550
           V N VT ++      W+ G   EDLN   L LFY  L +L+ LN L+Y++WA  Y
Sbjct: 503 VTNAVTGRL---GHQWLGG---EDLNKTRLELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of Lcy08g013950 vs. ExPASy TrEMBL
Match: A0A6J1FIK0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111444459 PE=3 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 8.7e-297
Identity = 517/595 (86.89%), Postives = 558/595 (93.78%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E +EI VV P +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALAT+FN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +VIGAAVGVT+IVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVA+KNR LALP+TP+ELYE++DK Y DSI SKIAHTNQLR LDKA+IL KD EP+ 
Sbjct: 243 QVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQP 302

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT+IMD+S+GHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRHQAT HP++PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNV+NK+T+K++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 542 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDAKAHHSEEKE 596
           YLYWASWYKYKTEE I +L+  GGG  E EEGVP+LREEE SG D K HHSEEKE
Sbjct: 543 YLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKE 597

BLAST of Lcy08g013950 vs. ExPASy TrEMBL
Match: A0A6J1HY86 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111468570 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.4e-293
Identity = 515/595 (86.55%), Postives = 551/595 (92.61%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCV GRIAFVFYTSL LLAIGSGGVRGALPALGADQF+QKDPKEAKALAT+FN +LLS
Sbjct: 123 PKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +VIGAAVGVTLIVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVAIKNR LALP TPSELYE++DK Y DSI SKIA T QLRFLDKA+IL KD EP+ 
Sbjct: 243 QVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEPQP 302

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT+IMD+S+GHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFPAP 362

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVE+
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEM 422

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRHQAT HP++PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNV+NKVT+K++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 542 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDAKAHHSEEKE 596
           YLYWASWYKYKTEEPI +LN  GGG  E EE VP+L EE   G   K HHSEEKE
Sbjct: 543 YLYWASWYKYKTEEPIVKLNDGGGGEREREESVPILHEERSGGA-IKCHHSEEKE 596

BLAST of Lcy08g013950 vs. ExPASy TrEMBL
Match: A0A6J1DQ47 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like OS=Momordica charantia OX=3673 GN=LOC111023287 PE=3 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.6e-287
Identity = 516/625 (82.56%), Postives = 552/625 (88.32%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E REIK VDP ++GKGGFRAT+FIFALL+FE+MGFVAN VSLVQYFLLV+HFDLQTAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTT L+FGVLEILALVM+TVQAYS DLLPKPDC
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
            KDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGA QFDQKDP+EAKAL T+FN MLLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +V+GAAVGVT+IVWVAVNKAW+WGF  S VATAVGF+VFA+GKPFYRLHVPG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVAI+NRRL LP++PSELYEI+DK Y DSI SKIAHTNQLRFLDKAAIL  D E +A
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT IMDRS+GHLQFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRR+QAT HP +PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNVVNKVT+ I PSK+GWVEG IPEDLNHNNLNLFYWFLAILSILNF H
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 542 YLYWASWYKYKTEEPIAEL-----------------NGEGGG-------------GEGEE 597
           YLYWASWYKYKTEEPIAEL                 NG GGG             GEGEE
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQDNRAEGEGEE 670

BLAST of Lcy08g013950 vs. ExPASy TrEMBL
Match: A0A5A7ULM1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00540 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 6.4e-284
Identity = 506/664 (76.20%), Postives = 552/664 (83.13%), Query Frame = 0

Query: 1   MGTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTA 60
           MGTE ++IKVVDP+DEGKGGFRATMFIF LLTFESMGFVAN  SLVQYFL V+HFDL+TA
Sbjct: 1   MGTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETA 60

Query: 61  ANTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPD 120
           ANTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FG LE++AL+++TVQAYSHDLLP P 
Sbjct: 61  ANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPL 120

Query: 121 CPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLL 180
           CPKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKAL T+FNYMLL
Sbjct: 121 CPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLL 180

Query: 181 SIVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRI 240
           S+V+GAAVGVT+IVWVAVNKAWYWGFF S +A  VGFI+FAIGKPFYR+ VPGQSP+LR+
Sbjct: 181 SVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRV 240

Query: 241 IQVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPE 300
           IQVIVVAIKNRRL LP+TP+ELYEI+DK Y DSIH KI HTNQLRFLDKAAI+ KD EP+
Sbjct: 241 IQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQ 300

Query: 301 AWKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPA 360
            W VCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT IMD+S+GH QFPA
Sbjct: 301 PWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPA 360

Query: 361 PSIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVE 420
           PSIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAI+MAVAGLVE
Sbjct: 361 PSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVE 420

Query: 421 VKRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWL 480
           VKRRHQA  HP++P+SLFWL+FQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFT+L
Sbjct: 421 VKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFL 480

Query: 481 SLSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFL 540
           SL+LGYYLSSIFVN+VNKVT+ ITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILS+LNF 
Sbjct: 481 SLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFF 540

Query: 541 HYLYWASWYKYKTEEPIAELNGEG------------------------------------ 597
           HYLYWASWYKYKTEEPI ELN  G                                    
Sbjct: 541 HYLYWASWYKYKTEEPIEELNSRGGETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKE 600

BLAST of Lcy08g013950 vs. ExPASy TrEMBL
Match: A0A1S4E0E0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo OX=3656 GN=LOC103493841 PE=3 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.4e-283
Identity = 505/663 (76.17%), Postives = 552/663 (83.26%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           GTE ++IKVVDP+DEGKGGFRATMFIF LLTFESMGFVAN  SLVQYFL V+HFDL+TAA
Sbjct: 17  GTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAA 76

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FG LE++AL+++TVQAYSHDLLP P C
Sbjct: 77  NTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLC 136

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKAL T+FNYMLLS
Sbjct: 137 PKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLS 196

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +V+GAAVGVT+IVWVAVNKAWYWGFF S +A  VGFI+FAIGKPFYR+ VPGQSP+LR+I
Sbjct: 197 VVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVI 256

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVAIKNRRL LP+TP+ELYEI+DK Y DSIH KI HTNQLRFLDKAAI+ KD EP+ 
Sbjct: 257 QVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQP 316

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           W VCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT IMD+S+GH QFPAP
Sbjct: 317 WNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAP 376

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAI+MAVAGLVEV
Sbjct: 377 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEV 436

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRHQA  HP++P+SLFWL+FQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFT+LS
Sbjct: 437 KRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLS 496

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           L+LGYYLSSIFVN+VNKVT+ ITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILS+LNF H
Sbjct: 497 LALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFH 556

Query: 542 YLYWASWYKYKTEEPIAELNGEG------------------------------------- 597
           YLYWASWYKYKTEEPI ELN +G                                     
Sbjct: 557 YLYWASWYKYKTEEPIEELNSKGGETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEP 616

BLAST of Lcy08g013950 vs. NCBI nr
Match: KAG6586059.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.0e-297
Identity = 517/595 (86.89%), Postives = 558/595 (93.78%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALAT+FN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +VIGAAVGVT+IVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVA+KNR LALP+TP+ELYE++DK Y DSI SKI HTNQLR LDKA+IL KD EP+ 
Sbjct: 243 QVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKITHTNQLRCLDKASILPKDVEPQP 302

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT+IMD+S+GHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRHQAT HP++PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNV+NK+T+K++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 542 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDAKAHHSEEKE 596
           YLYWASWYKYKTEE I +L+  GGG  E EEGVP+LREEE SG D K HHSEEKE
Sbjct: 543 YLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKE 597

BLAST of Lcy08g013950 vs. NCBI nr
Match: XP_023537955.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.0e-296
Identity = 521/595 (87.56%), Postives = 557/595 (93.61%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFLLVLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLLVLHFDLQTAA 62

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALAT+FN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +VIGAAVGVTLIVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVAIKNR LALP+TPSELYE+++K Y DSI SKIAHTNQLR LDKA+IL KD EP+ 
Sbjct: 243 QVIVVAIKNRGLALPDTPSELYELSNKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQP 302

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT+IMD+S+GHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRH+AT HP +PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 423 KRRHRATKHPNEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNV+NKVT+K++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 542 YLYWASWYKYKTEEPIAEL-NGEGGGGEGEEGVPVLREEEGSGVDAKAHHSEEKE 596
           YLYWASWYKYKTEE I +L NG GG  E EEGVP+LREEE SG D K HHSE KE
Sbjct: 543 YLYWASWYKYKTEETIVKLDNGGGGEREREEGVPILREEERSGGDIKCHHSEGKE 597

BLAST of Lcy08g013950 vs. NCBI nr
Match: XP_022938323.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.8e-296
Identity = 517/595 (86.89%), Postives = 558/595 (93.78%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E +EI VV P +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALAT+FN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +VIGAAVGVT+IVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVA+KNR LALP+TP+ELYE++DK Y DSI SKIAHTNQLR LDKA+IL KD EP+ 
Sbjct: 243 QVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQP 302

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT+IMD+S+GHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRHQAT HP++PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNV+NK+T+K++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 542 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDAKAHHSEEKE 596
           YLYWASWYKYKTEE I +L+  GGG  E EEGVP+LREEE SG D K HHSEEKE
Sbjct: 543 YLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKE 597

BLAST of Lcy08g013950 vs. NCBI nr
Match: XP_022969591.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 1016.9 bits (2628), Expect = 7.0e-293
Identity = 515/595 (86.55%), Postives = 551/595 (92.61%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
           PKDCV GRIAFVFYTSL LLAIGSGGVRGALPALGADQF+QKDPKEAKALAT+FN +LLS
Sbjct: 123 PKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +VIGAAVGVTLIVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVAIKNR LALP TPSELYE++DK Y DSI SKIA T QLRFLDKA+IL KD EP+ 
Sbjct: 243 QVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEPQP 302

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT+IMD+S+GHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFPAP 362

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSA+SMAVAGLVE+
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEM 422

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRRHQAT HP++PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNV+NKVT+K++PSK+GWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 542 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDAKAHHSEEKE 596
           YLYWASWYKYKTEEPI +LN  GGG  E EE VP+L EE   G   K HHSEEKE
Sbjct: 543 YLYWASWYKYKTEEPIVKLNDGGGGEREREESVPILHEERSGGA-IKCHHSEEKE 596

BLAST of Lcy08g013950 vs. NCBI nr
Match: XP_022156380.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like [Momordica charantia])

HSP 1 Score: 998.0 bits (2579), Expect = 3.4e-287
Identity = 516/625 (82.56%), Postives = 552/625 (88.32%), Query Frame = 0

Query: 2   GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 61
           G+E REIK VDP ++GKGGFRAT+FIFALL+FE+MGFVAN VSLVQYFLLV+HFDLQTAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 62  NTLTNFFGSTFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 121
           NTLTNF GS FLLSLLGGFLSDTYINRLTT L+FGVLEILALVM+TVQAYS DLLPKPDC
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 122 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLS 181
            KDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGA QFDQKDP+EAKAL T+FN MLLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 182 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 241
           +V+GAAVGVT+IVWVAVNKAW+WGF  S VATAVGF+VFA+GKPFYRLHVPG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 242 QVIVVAIKNRRLALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEA 301
           QVIVVAI+NRRL LP++PSELYEI+DK Y DSI SKIAHTNQLRFLDKAAIL  D E +A
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 302 WKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAP 361
           WKVCSVTQVEEVKIITRM PIF  TIIMNTCLAQLQTFSVEQGNT IMDRS+GHLQFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 362 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 421
           SIPVIPL+FM FLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 422 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 481
           KRR+QAT HP +PMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP+GMRSLSTSFTWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 482 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 541
           LS GYYLSSIFVNVVNKVT+ I PSK+GWVEG IPEDLNHNNLNLFYWFLAILSILNF H
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 542 YLYWASWYKYKTEEPIAEL-----------------NGEGGG-------------GEGEE 597
           YLYWASWYKYKTEEPIAEL                 NG GGG             GEGEE
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQDNRAEGEGEE 670

BLAST of Lcy08g013950 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 3.0e-116
Identity = 235/550 (42.73%), Postives = 337/550 (61.27%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F  + FLL+LLG
Sbjct: 25  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 84

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK------DCVRGRIAF 138
           GFL+D + +     L+   +E L L+++T+QA    L+P P C        + V G  A 
Sbjct: 85  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 144

Query: 139 VFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTL 198
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K  +T+FNY +  +  GA V VT 
Sbjct: 145 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 204

Query: 199 IVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVI----VVAI 258
           +VW+  NK W WGF  ST++  +  +VF +G  FY+  +P  SP+  I +V+    +V+ 
Sbjct: 205 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSC 264

Query: 259 KNRRLALPETPSELY-EINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEAWKVCSV 318
            ++  +   T  E+  E  +K  + S+      TN L  L+K AI GK      W  C+V
Sbjct: 265 SSKTSSNHFTSREVQSEHEEKTPSQSL------TNSLTCLNK-AIEGKTH--HIWLECTV 324

Query: 319 TQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAPSIPVIP 378
            QVE+VKI+ +M PIF CTI++N CLAQL T+SV Q  T  M+R I +   P+ S+PV P
Sbjct: 325 QQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFP 384

Query: 379 LLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEVKRRHQA 438
           ++FM  L P Y+ + +PFARK+T    GIT LQR+GVGLVLS ++MAVA LVE+KR+  A
Sbjct: 385 VVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVA 444

Query: 439 ------TTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 498
                  +    P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W S
Sbjct: 445 REAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWAS 504

Query: 499 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 552
           L+LGYYLSS+ V +VN+VTK    S   W    + E LN N L+LFYW + +LS++NFLH
Sbjct: 505 LALGYYLSSVMVPIVNRVTKSAGQSP--W----LGEKLNRNRLDLFYWLMCVLSVVNFLH 556

BLAST of Lcy08g013950 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 3.0e-116
Identity = 235/550 (42.73%), Postives = 337/550 (61.27%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F  + FLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK------DCVRGRIAF 138
           GFL+D + +     L+   +E L L+++T+QA    L+P P C        + V G  A 
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 148

Query: 139 VFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTL 198
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K  +T+FNY +  +  GA V VT 
Sbjct: 149 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 208

Query: 199 IVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVI----VVAI 258
           +VW+  NK W WGF  ST++  +  +VF +G  FY+  +P  SP+  I +V+    +V+ 
Sbjct: 209 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSC 268

Query: 259 KNRRLALPETPSELY-EINDKCYTDSIHSKIAHTNQLRFLDKAAILGKDREPEAWKVCSV 318
            ++  +   T  E+  E  +K  + S+      TN L  L+K AI GK      W  C+V
Sbjct: 269 SSKTSSNHFTSREVQSEHEEKTPSQSL------TNSLTCLNK-AIEGKTH--HIWLECTV 328

Query: 319 TQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAPSIPVIP 378
            QVE+VKI+ +M PIF CTI++N CLAQL T+SV Q  T  M+R I +   P+ S+PV P
Sbjct: 329 QQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFP 388

Query: 379 LLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEVKRRHQA 438
           ++FM  L P Y+ + +PFARK+T    GIT LQR+GVGLVLS ++MAVA LVE+KR+  A
Sbjct: 389 VVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVA 448

Query: 439 ------TTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 498
                  +    P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TS +W S
Sbjct: 449 REAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWAS 508

Query: 499 LSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLH 552
           L+LGYYLSS+ V +VN+VTK    S   W    + E LN N L+LFYW + +LS++NFLH
Sbjct: 509 LALGYYLSSVMVPIVNRVTKSAGQSP--W----LGEKLNRNRLDLFYWLMCVLSVVNFLH 560

BLAST of Lcy08g013950 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 405.6 bits (1041), Expect = 6.9e-113
Identity = 226/566 (39.93%), Postives = 337/566 (59.54%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG  A  F+  +   E++ ++AN  +LV Y    +H     +AN +TNF G+ FLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK---DCVRGRIAFVFY 138
           GFLSD + +     L+   +E L L+++T+QA +  L+P P C     + V G  A + +
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMP-PSCDSPTCEEVSGSKAAMLF 146

Query: 139 TSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTLIVW 198
             LYL+A+G GG++G+L + GA+QFD+  PK  K  +T+FNY +  +  GA V VT +VW
Sbjct: 147 VGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVW 206

Query: 199 VAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVA-------- 258
           +  NK W WGF  ST+A  V  ++F  G  FYR  +P  SP+  I++V++ A        
Sbjct: 207 LEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG 266

Query: 259 -IKNRRLALPETPS------------ELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILG 318
              N   ++  +PS               E+      +++  +   TN L+ L+ AA   
Sbjct: 267 SSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA--- 326

Query: 319 KDREP-EAWKVCSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSI 378
            D +P      C+V QVE+VKI+ +M PIF CTI++N CLAQL TFSV+Q  +  M+  I
Sbjct: 327 -DEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKI 386

Query: 379 GHLQFPAPSIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISM 438
           G L+ P  S+P+ P++F+  L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS ++M
Sbjct: 387 GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAM 446

Query: 439 AVAGLVEVKRRHQA------TTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAP 498
           AVA LVE+KR+  A       +    P++  W++ QY   G AD+FTL GLLE+F+ EAP
Sbjct: 447 AVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAP 506

Query: 499 MGMRSLSTSFTWLSLSLGYYLSSIFVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLF 554
             MRSL+TS +W SL++GYYLSS+ V++VN +T   +     W+ G   + +N   L+ F
Sbjct: 507 SSMRSLATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYF 566

BLAST of Lcy08g013950 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 2.6e-112
Identity = 229/537 (42.64%), Postives = 327/537 (60.89%), Query Frame = 0

Query: 19  GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFLLSLLG 78
           GG RA +F+  L  FE MG  A G +L+ Y +  +HF L  AAN +TNF G+ F+ +LLG
Sbjct: 43  GGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLG 102

Query: 79  GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLP---KPDCPKDC--VRGRIAFV 138
           G+LSD ++    T ++FG +E+   ++++VQA+   L P    P   + C   +G  A +
Sbjct: 103 GYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMI 162

Query: 139 FYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGAAVGVTLI 198
           F+ +LYL+A+GSG V+  + A GADQF Q  PK++K L++YFN    +  +G  + +TL+
Sbjct: 163 FFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLL 222

Query: 199 VWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVAIKNRRL 258
           VWV  +     GF  S  A  +G I    G  ++R   P +S    I  VIV AI  R+L
Sbjct: 223 VWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKL 282

Query: 259 ALPETPSELYEINDKCYTDSIHSKIAHTNQLRFLDKAAILGKD--REPEAWKVCSVTQVE 318
           A P  P  L+  +         S + HT + RFLDKA I  +D   +   W++C+VTQVE
Sbjct: 283 ASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVE 342

Query: 319 EVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDRSIGHLQFPAPSIPVIPLLFM 378
           +VK +  + PIF  TI+ NT LAQLQTFSV+QG++ +  R       P  S+  IP + +
Sbjct: 343 QVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSS-MNTRLSNSFHIPPASLQAIPYIML 402

Query: 379 TFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEVKRRHQATTHP 438
            FL+PLY+   VPFARK+T H SGI  L R+G+GL LS  SM  A ++E KRR  ++   
Sbjct: 403 IFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRR-DSSVLD 462

Query: 439 EKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLSLSLGYYLSSI 498
            + +S+FW++ Q+ IFGI++MFT VGL+EFFYK++  GM S   + T+ S S G+Y SS+
Sbjct: 463 GRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSV 522

Query: 499 FVNVVNKVTKKITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFLHYLYWASW 549
            V+VVNK+T     SK GW   L   DLN + L+LFYW LA+LS+LNFL YL+W+ W
Sbjct: 523 LVSVVNKITSTSVDSK-GW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Lcy08g013950 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.3e-108
Identity = 233/549 (42.44%), Postives = 328/549 (59.74%), Query Frame = 0

Query: 14  RDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSTFL 73
           R    GG RA +F+     FE M   A G +L+ Y    +HF L  +AN +TNF G+ FL
Sbjct: 35  RPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFL 94

Query: 74  LSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK-----DCV-- 133
           LSLLGGFLSD+Y+    T LVFGV+EI   ++++VQA+  +L P P+C        CV  
Sbjct: 95  LSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP-PECNMKSTTIHCVEA 154

Query: 134 RGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATYFNYMLLSIVIGA 193
            G  A   YT+L L+A+GSG ++  + + GA+QF +KD    + L+++FN    +  +G 
Sbjct: 155 NGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQ 214

Query: 194 AVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVV 253
            + +TL+VWV  +     GF  S    A G I    G  FYR   P  S    I QV V 
Sbjct: 215 LIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVA 274

Query: 254 AIKNRRLALPETPSELYEINDKCYTDSIHSK-IAHTNQLRFLDKAAILGKDREPEA-WKV 313
           AI  R+   P  P+ +++ +    TD +  K + H+N+ RFLDKA I  + +  E+ W++
Sbjct: 275 AITKRKQICPSNPNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRL 334

Query: 314 CSVTQVEEVKIITRMFPIFFCTIIMNTCLAQLQTFSVEQG---NTRIMDRSIGHLQFPAP 373
           C++ QV +VKI+  + PIF CTII NT LAQLQTFSV+QG   NT I        Q P  
Sbjct: 335 CTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPA 394

Query: 374 SIPVIPLLFMTFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 433
           S+  IP + + F +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  SM  A LVE 
Sbjct: 395 SLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEK 454

Query: 434 KRRHQATTHPEKPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPMGMRSLSTSFTWLS 493
           KRR ++       +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S
Sbjct: 455 KRR-ESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCS 514

Query: 494 LSLGYYLSSIFVNVVNKVTKKI-TPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSILNFL 550
            S G+YLSS+ V+ VN+VT    + +K GW   L   DLN + L+ FYW LA LS +NF 
Sbjct: 515 YSFGFYLSSVLVSTVNRVTSSNGSGTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFF 567