Lcy08g013350 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g013350
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRibonuclease H-like superfamily protein
LocationChr08: 43176363 .. 43178827 (+)
RNA-Seq ExpressionLcy08g013350
SyntenyLcy08g013350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGCTAAGCAGGTCTGGAGGGTGTTGTGCAACCCTCAGCTAAAAATTTCTCGAGTCCTTTGTGGGAGATATTTCCCGAATTCAATGGTTTTAAATGCAACTTCTCGTTCGTCTTTCTCTTTCTTTTGGAAGGGTTTTGTTTGGGGAATGGAACTTCTGAAAAAAGGTATTCGAAAGAACATGGGGAATGGGAGATCAATATATATGTACAGGGACCCCTGGATTCCGAGACCAACGACCTTTAAGGTAATCTCATATCCTAGCCCGACTATGGAGAATGTGGTAGTGGTGGAGTTTATAAAGCATAACGGCCAATGGGACTTGGAGAAACTGGACCAACACCTGGATAGGGTGGATGTTGATGTGATCAAAAGTCTCCCGATAAGTAGTCATGCTCCAGACAGATGGATCTGGCATTATGATAGGAGAGGAGAGTACTCGGTGAAAAGTGGTTATAAGCTCTGTATAAGGGATGGTCGAGAGGCGTCGTCATCAGATAGTGGGGTGGAGTATAGTTGGTGGATTAACCTATGGAAATTGAGAGTTCCTAGTAAGGTGAAACATTTTGTATGGAGATCTTTCAATAATTCAATTCCAACCATGGTTAATCTGTTGAATCATCATGTTCCGGTCAATGGGGTTTGTCCAATGTGTAAGGAGGAGAATGAAACTACGGATCATGCCCTTTTCTTGTGTAATCGAGCCCAAGAGGTTTGGAAGACTCGTTTTCCGACAGGGTTTGGGATTGGTTTTGATCCTATGGAGATTAAAGACAAATGGATGTGGCTGAGTAGGGCGAATGGACCGCTCTTAGAGCGTATTTGTGTTGGTGCCTGGGCAATCTGGAATGATAGAAATAATCTGGTGCACAATCGTCCAATCCCTTCTGTCAATTTGCGATGTGAATGGATAAATTCCTATTTGGAAGAATATTGGCAGGCAAACCCAAAGGGGAATCCATGTGTGCAAACAGAGGAGGACATCTCCAACATCATTTATAATGGGGAAGATTTCATTCTTCATACGGATGCGGCATTCATGGGTGGCTCTTGTGGTAGTGGAGTTGGTCTAGTTCTTCGGAATAAATTAGGACAACTACAGGCAGCGCAATCATTAGCTACAGCGGTTTGTACATCTCCTTTGGGGGTGGAAGCTGTTGCTATCCTTGAAGGTCTTCGCTTGGCCTAATTGATGGGGGTGAGTAAAGGTTACGGTGTTGTTAGACTCCATGAATTTAATCAAGACATTAAAAGGTGAGATGCAGGGTGACGCAAGCATTTCTACGACGCTTTGGGATATCCAAGCGCTTGCAGCCTCTTTTGAAAAGGTGGTTTTTAATTTTGTAAGTCGTAGTTTCAATGTATTTGCCCATAAAATGGCCAAACTAGGCCTCTCGTCTACGCCAAATTTGTGGCTGCGGAACTATTCTCAATGGATGGTCAGTTTATCAATTAGTGAGCTCTATATGTCCAAATCCCTTAGGGGATCTTTTGTTTCTTAATGGACGTTCCGGTTTCTCAAAAAAAAAAAAAAAAAAAGAATGATCATTTATGAATATGATCTTTTATTTTTAGTTTTTGGAAAATTATTTATTTATATTTTACCTGGACATTAAATTTGGATGAGTAAGAACATTAATGCTTTTGATTGGTCCAAGTTTTCTTCTTTTGTGAATGATGATTGAATGAGATTACACTTTGGATAAAACTTCTCTGCACAACGGTGAGCAGCAATTTCCTACTTACCATCACTATTCTTTTAACATATGTCACTTTCTTATTTTTTATTTACTTTTATTAAATGTCAAAATCTCAACAAAATCTCATCAAAATCTCATCTAACAGATTTTACTTTTTATTCCATTTTTTTAATTTAAAACACGTTCTTCTCTCCCTTCATTTTTAATGCTTCAGATCCCCTTCTCTCTCTTTTTCTTTTGAATTTATTGTATCCTTCCAACATACACCTCATCATCTCTCATCTTCTCACATTTCTCATTCTTTCCTCTATCATTCCACAAAAAAATTCAGTATGATTTTGCACAAGCTAAAGGTAACCTAAGTTAAACTCTTTTGTTTTTTTTTTTTAATATATTGTGTATTTTTTATATTTTTTTATAGTATCTTTTAAATGAAATAATATCATCTATTTTCAATAGTTCAACTAAATTGGGATGACTAAAGGAATCATGTGAGAGTGACAATCTCATAATCCAGAATAAACAACTCGCTTGAAACAAGTGAGTTTGTTATTTTATTTCATTAATTTATATTTTTATCAATATTCATTTTTTTTAATTTTCAGATATGTTCATGTAACCTTGATTTTTTTTTTTTTTTTTTTAACGAAGTGACCCAATATTTGATTTTGAATTATTGGATTCATAATTAGAAAAACAAGTTTTATTTTTTGTTTTTACCATATCTGAAAAATGGGTTTGGTTGTAGATTGAAGAGATATAA

mRNA sequence

ATGATTGCTAAGCAGGTCTGGAGGGTGTTGTGCAACCCTCAGCTAAAAATTTCTCGAGTCCTTTGTGGGAGATATTTCCCGAATTCAATGGTTTTAAATGCAACTTCTCGTTCGTCTTTCTCTTTCTTTTGGAAGGGTTTTGTTTGGGGAATGGAACTTCTGAAAAAAGGTATTCGAAAGAACATGGGGAATGGGAGATCAATATATATGTACAGGGACCCCTGGATTCCGAGACCAACGACCTTTAAGGTAATCTCATATCCTAGCCCGACTATGGAGAATGTGGTAGTGGTGGAGTTTATAAAGCATAACGGCCAATGGGACTTGGAGAAACTGGACCAACACCTGGATAGGGTGGATGTTGATGTGATCAAAAGTCTCCCGATAAGTAGTCATGCTCCAGACAGATGGATCTGGCATTATGATAGGAGAGGAGAGTACTCGGTGAAAAGTGGTTATAAGCTCTGTATAAGGGATGGTCGAGAGGCGTCGTCATCAGATAGTGGGGTGGAGTATAGTTGGTGGATTAACCTATGGAAATTGAGAGTTCCTAGTAAGGTGAAACATTTTGTATGGAGATCTTTCAATAATTCAATTCCAACCATGGTTAATCTGTTGAATCATCATGTTCCGGTCAATGGGGTTTGTCCAATGTGTAAGGAGGAGAATGAAACTACGGATCATGCCCTTTTCTTGTGTAATCGAGCCCAAGAGGTTTGGAAGACTCGTTTTCCGACAGGGTTTGGGATTGGTTTTGATCCTATGGAGATTAAAGACAAATGGATGTGGCTGAGTAGGGCGAATGGACCGCTCTTAGAGCGTATTTGTGTTGGTGCCTGGGCAATCTGGAATGATAGAAATAATCTGGTGCACAATCGTCCAATCCCTTCTGTCAATTTGCGATGTGAATGGATAAATTCCTATTTGGAAGAATATTGGCAGGCAAACCCAAAGGGGAATCCATGTGTGCAAACAGAGGAGGACATCTCCAACATCATTTATAATGGGGAAGATTTCATTCTTCATACGGATGCGGCATTCATGGGTGGCTCTTGTGGTAGTGGAGTTGGTCTAGTTCTTCGGAATAAATTAGGACAACTACAGGCAGCGCAATCATTAGCTACAGCGATTGAAGAGATATAA

Coding sequence (CDS)

ATGATTGCTAAGCAGGTCTGGAGGGTGTTGTGCAACCCTCAGCTAAAAATTTCTCGAGTCCTTTGTGGGAGATATTTCCCGAATTCAATGGTTTTAAATGCAACTTCTCGTTCGTCTTTCTCTTTCTTTTGGAAGGGTTTTGTTTGGGGAATGGAACTTCTGAAAAAAGGTATTCGAAAGAACATGGGGAATGGGAGATCAATATATATGTACAGGGACCCCTGGATTCCGAGACCAACGACCTTTAAGGTAATCTCATATCCTAGCCCGACTATGGAGAATGTGGTAGTGGTGGAGTTTATAAAGCATAACGGCCAATGGGACTTGGAGAAACTGGACCAACACCTGGATAGGGTGGATGTTGATGTGATCAAAAGTCTCCCGATAAGTAGTCATGCTCCAGACAGATGGATCTGGCATTATGATAGGAGAGGAGAGTACTCGGTGAAAAGTGGTTATAAGCTCTGTATAAGGGATGGTCGAGAGGCGTCGTCATCAGATAGTGGGGTGGAGTATAGTTGGTGGATTAACCTATGGAAATTGAGAGTTCCTAGTAAGGTGAAACATTTTGTATGGAGATCTTTCAATAATTCAATTCCAACCATGGTTAATCTGTTGAATCATCATGTTCCGGTCAATGGGGTTTGTCCAATGTGTAAGGAGGAGAATGAAACTACGGATCATGCCCTTTTCTTGTGTAATCGAGCCCAAGAGGTTTGGAAGACTCGTTTTCCGACAGGGTTTGGGATTGGTTTTGATCCTATGGAGATTAAAGACAAATGGATGTGGCTGAGTAGGGCGAATGGACCGCTCTTAGAGCGTATTTGTGTTGGTGCCTGGGCAATCTGGAATGATAGAAATAATCTGGTGCACAATCGTCCAATCCCTTCTGTCAATTTGCGATGTGAATGGATAAATTCCTATTTGGAAGAATATTGGCAGGCAAACCCAAAGGGGAATCCATGTGTGCAAACAGAGGAGGACATCTCCAACATCATTTATAATGGGGAAGATTTCATTCTTCATACGGATGCGGCATTCATGGGTGGCTCTTGTGGTAGTGGAGTTGGTCTAGTTCTTCGGAATAAATTAGGACAACTACAGGCAGCGCAATCATTAGCTACAGCGATTGAAGAGATATAA

Protein sequence

MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRKNMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVDVDVIKSLPISSHAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLWKLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEVWKTRFPTGFGIGFDPMEIKDKWMWLSRANGPLLERICVGAWAIWNDRNNLVHNRPIPSVNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIYNGEDFILHTDAAFMGGSCGSGVGLVLRNKLGQLQAAQSLATAIEEI
Homology
BLAST of Lcy08g013350 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.7e-16
Identity = 66/259 (25.48%), Postives = 120/259 (46.33%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLN--ATSRSSFSFFWKGFVWGM-ELLKKG 60
           +I+K  WR+L       + VL  +Y    +  +     + S+S  W+    G+ +++  G
Sbjct: 113 LISKVGWRLLQEKNSLWTLVLQKKYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHG 172

Query: 61  IRKNMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQ-WDLEKLDQHL 120
           +    G+G+ I  + D W+      ++ +   PT  + VV + +   G+ WD  K+D + 
Sbjct: 173 VGWIPGDGQQIRFWTDRWVSGKPLLELDNGERPTDCDTVVAKDLWIPGRGWDFAKIDPYT 232

Query: 121 ---DRVDVDVIKSLPISSHAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYS 180
               R+++  +  L + + A DR  W + + G++SV+S Y++   D  E    +     S
Sbjct: 233 TNNTRLELRAV-VLDLVTGARDRLSWKFSQDGQFSVRSAYEMLTVD--EVPRPNMA---S 292

Query: 181 WWINLWKLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLC 240
           ++  LWK+RVP +VK F+W   N ++ T       H+  + VC +CK   E+  H L  C
Sbjct: 293 FFNCLWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDC 352

Query: 241 NRAQEVWKTRFPTGFGIGF 253
                +W    P     GF
Sbjct: 353 PAQLGIWVRVVPQRRQQGF 365

BLAST of Lcy08g013350 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 8.3e-08
Identity = 25/76 (32.89%), Postives = 47/76 (61.84%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++AKQ +R++  P   +SR+L  RYFP+S ++  +  +  S+ W+  + G ELL +G+ +
Sbjct: 69  LLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLR 128

Query: 61  NMGNGRSIYMYRDPWI 77
            +G+G    ++ D WI
Sbjct: 129 TIGDGIHTKVWLDRWI 144

BLAST of Lcy08g013350 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 4.5e-73
Identity = 142/381 (37.27%), Postives = 220/381 (57.74%), Query Frame = 0

Query: 1    MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
            ++AK VWR L +P L +S+VL  +YF ++ +L A++ S  S+FWKGF+WG +LL KG+R 
Sbjct: 958  LVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRL 1017

Query: 61   NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
             +GNG +I  + DPW+PRPTTFK + + +  ++   V  FI  +G WD+  +       D
Sbjct: 1018 RVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALD-TTVASFITADGNWDVTSISHSFCNED 1077

Query: 121  VDVIKSLPISSH-APDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
             D+I S+PISS+   D W+WHYD+RG YSV+SGYKL +     A+S+ +    + W ++W
Sbjct: 1078 RDLILSMPISSYNLQDSWLWHYDKRGNYSVRSGYKLYMHLKCNATSASTNYRGTQWNSIW 1137

Query: 181  KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
            KL VP+K+K F+WRS +  IPT  NLL   +     C +C +  E+  HA F C RA+++
Sbjct: 1138 KLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRESIIHAFFHCKRARQI 1197

Query: 241  WKTRFPTGFGIGF-DPMEIKDKWMWLSRANGPL-LERICVGAWAIWNDRNNLVHNRPIPS 300
            W+T FP    +   D +   + W  L+    P  L    +  W IWNDRN+L+H + +  
Sbjct: 1198 WRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHGKQVSP 1257

Query: 301  VNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIY-----NGEDFILHTDAAFMGGSC 360
            V  +CEW+  +L+ + QA    N   +T+ +   ++      +     L+TDAA  G S 
Sbjct: 1258 VEFKCEWLTPFLDSHSQAQ-MSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAACRGAS- 1317

Query: 361  GSGVGLVLRNKLGQLQAAQSL 374
             +  G ++R+    L AA S+
Sbjct: 1318 -TSFGCIIRDSSCSLVAATSI 1334

BLAST of Lcy08g013350 vs. ExPASy TrEMBL
Match: A0A803Q105 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 8.2e-59
Identity = 140/398 (35.18%), Postives = 204/398 (51.26%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++AKQ WR+   P   +SRVL GRY+P+S  + A++    S  W+G VWG ELL KG+R 
Sbjct: 45  LLAKQAWRIFQQPNSLLSRVLKGRYYPHSEFMTASANGLCSLTWQGIVWGRELLAKGLRL 104

Query: 61  NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
            +G+G SI    D WIP    FK   Y  P+  +  V ++I  + +W+++ L       D
Sbjct: 105 KVGSGLSIACGTDSWIPGHDNFKAFCYLGPSSNH--VSDYITTDREWNIDLLQADFSPPD 164

Query: 121 VDVIKSLPISSHA-PDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
           VD I ++P+S +A  DRWIWH+D  G+YSV +GY           S+ S  + +WW   W
Sbjct: 165 VDRILTIPLSYNAVQDRWIWHHDVSGDYSVSTGYHFASSLEEREISTGSNTQNTWWKTFW 224

Query: 181 KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
              +P+KVK F WR   +SIP   +L +  V  +  C +C+   ET  HALF C  A+EV
Sbjct: 225 SYNLPAKVKIFGWRVIQSSIPVAKSLFHKKVLTSATCSLCQTAWETIGHALFSCCHAKEV 284

Query: 241 WKTRFPTGFGIGFDP---MEIKDKWMWLSRANGPL-LERICVGAWAIWNDRNNLVHNRPI 300
           WK    +GF   F     M+  D  M+LS  +  L +ERI    W IW+DRNN +H +  
Sbjct: 285 WKL---SGFCFNFHSAHRMQDGDYLMYLSSLHTKLEMERILCVMWFIWSDRNNFIHGKKT 344

Query: 301 PS-VNLRCEWINSYLEEYWQANPKG--NPCVQTEEDIS----NIIYNGED---------- 360
              VNL  +  ++YL++Y   N     +  V T   IS    NI ++             
Sbjct: 345 KGPVNLLSQ-SDAYLQQYQLINQAALQHTGVHTSSAISASSPNIGFHTNSQAAVVKWHAP 404

Query: 361 ----FILHTDAAFMGGSCGSGVGLVLRNKLGQLQAAQS 373
                 ++ DAA        G+G+++RN  GQ+ AA S
Sbjct: 405 PENKIKMNVDAAIDSSRSKIGIGVIIRNSNGQVLAAMS 436

BLAST of Lcy08g013350 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.3e-58
Identity = 134/386 (34.72%), Postives = 197/386 (51.04%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++AKQ+WR L +PQL  SRVL   YFP   VL A   ++ SF W+  VWG +L+ KG R 
Sbjct: 212 LLAKQIWRCLRHPQLLCSRVLKASYFPRKGVLEAGCGANASFVWRSLVWGKKLILKGYRW 271

Query: 61  NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
            +GNG S+ +  DPW+PRP TFKV   PS    N+ V +    +GQWD   +    +  D
Sbjct: 272 RVGNGESVRVLEDPWLPRPVTFKVYDQPS-LPANLYVTDLKLADGQWDEGFIRSIFNPTD 331

Query: 121 VDVIKSLPISS-HAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
           VD+I  +P S     D+ +WHY + GEYSVKSGY++      E   S+      WW  LW
Sbjct: 332 VDLILGIPCSDWDFEDKILWHYSKYGEYSVKSGYRMAASFTTEQHQSNEHSIVQWWKKLW 391

Query: 181 KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEE-NETTDHALFLCNRAQE 240
           +L++P KVKHFVW+  +N +P  VNL    +  + VC  C    +E+  HAL+ C  ++ 
Sbjct: 392 RLKIPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRCSSHVDESVAHALWECKASKG 451

Query: 241 VWKTRFPTGFGIGFDPMEIKDKWMWLSRA----NGPLLERICVGAWAIWNDRNNLVHNRP 300
            W+    +G       M  +D    L R     +   LE   + +W IWN RN +VH   
Sbjct: 452 YWRV---SGLYDDLKQMLGEDNLTMLMRIAAEWDKEKLEFFLLVSWNIWNVRNTVVHGGY 511

Query: 301 IPSVNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIYNGEDFI-LHTDAAFMGGSCG 360
            P      EW  ++L ++      G    Q   + S  +    D + ++ DA    G   
Sbjct: 512 HPKPEEMIEWCGNFLADF--RGDTGRERSQRSSEDSRWVPPARDQVTINVDAGVKQGGLI 571

Query: 361 SGVGLVLRNKLGQLQAAQSLATAIEE 380
           SG+G V+R+  G + +A   AT +++
Sbjct: 572 SGLGYVVRDAAGVVLSA--AATVLQQ 589

BLAST of Lcy08g013350 vs. ExPASy TrEMBL
Match: A0A803QGT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 7.0e-58
Identity = 132/381 (34.65%), Postives = 189/381 (49.61%), Query Frame = 0

Query: 1    MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
            ++AKQ WR+  +P   +SRVL G YF  +  + A      S  W+G VWG ELL KG+R 
Sbjct: 830  LLAKQAWRIFQDPTSLLSRVLKGHYFSQNDFMTARGGGLSSLTWQGIVWGRELLVKGLRL 889

Query: 61   NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
             +G G +I    D WIP    FK   Y      +  V ++I    +W++E L       D
Sbjct: 890  KVGTGNNIRCAVDSWIPGHKGFKPYCYTGAHTNH--VADYITATREWNIECLQSDFSTPD 949

Query: 121  VDVIKSLPIS-SHAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
            VD I  +P+S     DRWIWHY+  G+YSV SGY L    G E  SS S  + +WW + W
Sbjct: 950  VDNILKIPLSFLPVNDRWIWHYEDSGDYSVSSGYTLASSLGEEDLSSCSHTQETWWKSFW 1009

Query: 181  KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
            KL +PSKVK F W+   +SIP   +L +  +  +  C +C+   E+  HALF C  A+EV
Sbjct: 1010 KLNLPSKVKIFGWKVIQSSIPVAKSLYHRKILTSATCSLCQSAWESIGHALFSCCHAKEV 1069

Query: 241  WKTRFPTGFGIGF---DPMEIKDKWMWLSRA-NGPLLERICVGAWAIWNDRNNLVHNRPI 300
            WK    +GF I F   D ++  D  M LS        E I    W IW+DRNN +H + +
Sbjct: 1070 WKF---SGFSIDFTNADRLQDGDYLMHLSSIYEKSAFENILCLMWFIWSDRNNFIHGKKV 1129

Query: 301  PSVNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIY----NGEDFILHTDAAFMGGS 360
             +         +Y+++Y        P        +++          F L+ DAA     
Sbjct: 1130 KTPLQMWTQSVAYMDQYRSITSAVTPAASNRTSQASVASWKPPPENTFKLNVDAALDSSR 1189

Query: 361  CGSGVGLVLRNKLGQLQAAQS 373
               G+G+++RN  GQ++AA S
Sbjct: 1190 SKIGIGVIVRNSAGQVKAALS 1205

BLAST of Lcy08g013350 vs. ExPASy TrEMBL
Match: A0A803Q185 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 3.5e-57
Identity = 125/380 (32.89%), Postives = 195/380 (51.32%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++AKQ+WR +  P    +RVL   YFP + VL A   +  SF W+  +WG +L+ KG R 
Sbjct: 90  LLAKQIWRCIRYPNALCNRVLKASYFPTNSVLEAKCGTHASFVWRSLMWGKKLILKGYRW 149

Query: 61  NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
            +G+G +I +  DPW+PRP TFK+   P P  E++ VV+    +G WD   +    ++ D
Sbjct: 150 RVGDGSNIRVLEDPWLPRPVTFKIYDKP-PLPEHLWVVDLKLGDGTWDKPFISAVFNKDD 209

Query: 121 VDVIKSLPISS-HAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
            ++I +LP S     D+ +WHY + GEYSVKSGY++      E   SD  +   WW  LW
Sbjct: 210 AEMILALPNSGWDLDDKILWHYCKNGEYSVKSGYRMACELKAERQQSDDHLAVQWWRKLW 269

Query: 181 KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEEN-ETTDHALFLCNRAQE 240
           +L++P K+K FVW+  +  +PT   L   HV     C  C   N ET  HAL+ C +++ 
Sbjct: 270 RLKIPPKIKVFVWKLAHGWLPTSTVLAKRHVSTTDGCSRCSLHNSETIFHALWECKKSKV 329

Query: 241 VWKTRFPTGFGIGFDPMEIKDKWMWLSRANGPL----LERICVGAWAIWNDRNNLVHNRP 300
            WK      F +       +D+  +L R +  +     E   V  W++WN RN   H+  
Sbjct: 330 CWKL---CDFQLEIKRHGQEDELAFLMRLSTVMSKDQFEMFLVITWSLWNTRNACTHDGF 389

Query: 301 IPSVNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIYNGEDFILHTDAAFMGGSCGS 360
           +P      EW    LE++     +    ++ EE +  +  +GE   ++ DA+   G+  S
Sbjct: 390 VPQPAEMVEWCYKLLEDFQGGRSRPQEVMRREEGVWKVPRHGE-VKINVDASVKTGAGYS 449

Query: 361 GVGLVLRNKLGQLQAAQSLA 375
           G+G V+R + G +  A S A
Sbjct: 450 GLGCVIRGEQGDVLFASSTA 464

BLAST of Lcy08g013350 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 285.0 bits (728), Expect = 9.3e-73
Identity = 142/381 (37.27%), Postives = 220/381 (57.74%), Query Frame = 0

Query: 1    MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
            ++AK VWR L +P L +S+VL  +YF ++ +L A++ S  S+FWKGF+WG +LL KG+R 
Sbjct: 958  LVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRL 1017

Query: 61   NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
             +GNG +I  + DPW+PRPTTFK + + +  ++   V  FI  +G WD+  +       D
Sbjct: 1018 RVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALD-TTVASFITADGNWDVTSISHSFCNED 1077

Query: 121  VDVIKSLPISSH-APDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
             D+I S+PISS+   D W+WHYD+RG YSV+SGYKL +     A+S+ +    + W ++W
Sbjct: 1078 RDLILSMPISSYNLQDSWLWHYDKRGNYSVRSGYKLYMHLKCNATSASTNYRGTQWNSIW 1137

Query: 181  KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
            KL VP+K+K F+WRS +  IPT  NLL   +     C +C +  E+  HA F C RA+++
Sbjct: 1138 KLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDRRESIIHAFFHCKRARQI 1197

Query: 241  WKTRFPTGFGIGF-DPMEIKDKWMWLSRANGPL-LERICVGAWAIWNDRNNLVHNRPIPS 300
            W+T FP    +   D +   + W  L+    P  L    +  W IWNDRN+L+H + +  
Sbjct: 1198 WRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHGKQVSP 1257

Query: 301  VNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIY-----NGEDFILHTDAAFMGGSC 360
            V  +CEW+  +L+ + QA    N   +T+ +   ++      +     L+TDAA  G S 
Sbjct: 1258 VEFKCEWLTPFLDSHSQAQ-MSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAACRGAS- 1317

Query: 361  GSGVGLVLRNKLGQLQAAQSL 374
             +  G ++R+    L AA S+
Sbjct: 1318 -TSFGCIIRDSSCSLVAATSI 1334

BLAST of Lcy08g013350 vs. NCBI nr
Match: XP_030479133.1 (uncharacterized protein LOC115696372 [Cannabis sativa])

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-57
Identity = 132/381 (34.65%), Postives = 189/381 (49.61%), Query Frame = 0

Query: 1    MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
            ++AKQ WR+  +P   +SRVL G YF  +  + A      S  W+G VWG ELL KG+R 
Sbjct: 829  LLAKQAWRIFQDPTSLLSRVLKGHYFSQNDFMTARGGGLSSLTWQGIVWGRELLVKGLRL 888

Query: 61   NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
             +G G +I    D WIP    FK   Y      +  V ++I    +W++E L       D
Sbjct: 889  KVGTGNNIRCAVDSWIPGHKGFKPYCYTGAHTNH--VADYITATREWNIECLQSDFSTPD 948

Query: 121  VDVIKSLPIS-SHAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
            VD I  +P+S     DRWIWHY+  G+YSV SGY L    G E  SS S  + +WW + W
Sbjct: 949  VDNILKIPLSFLPVNDRWIWHYEDSGDYSVSSGYTLASSLGEEDLSSCSHTQETWWKSFW 1008

Query: 181  KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
            KL +PSKVK F W+   +SIP   +L +  +  +  C +C+   E+  HALF C  A+EV
Sbjct: 1009 KLNLPSKVKIFGWKVIQSSIPVAKSLYHRKILTSATCSLCQSAWESIGHALFSCCHAKEV 1068

Query: 241  WKTRFPTGFGIGF---DPMEIKDKWMWLSRA-NGPLLERICVGAWAIWNDRNNLVHNRPI 300
            WK    +GF I F   D ++  D  M LS        E I    W IW+DRNN +H + +
Sbjct: 1069 WKF---SGFSIDFTNADRLQDGDYLMHLSSIYEKSAFENILCLMWFIWSDRNNFIHGKKV 1128

Query: 301  PSVNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIY----NGEDFILHTDAAFMGGS 360
             +         +Y+++Y        P        +++          F L+ DAA     
Sbjct: 1129 KTPLQMWTQSVAYMDQYRSITSAVTPAASNRTSQASVASWKPPPENTFKLNVDAALDSSR 1188

Query: 361  CGSGVGLVLRNKLGQLQAAQS 373
               G+G+++RN  GQ++AA S
Sbjct: 1189 SKIGIGVIVRNSAGQVKAALS 1204

BLAST of Lcy08g013350 vs. NCBI nr
Match: XP_030483481.1 (uncharacterized protein LOC115700065 [Cannabis sativa])

HSP 1 Score: 233.8 bits (595), Expect = 2.5e-57
Identity = 135/388 (34.79%), Postives = 199/388 (51.29%), Query Frame = 0

Query: 1    MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
            ++AKQ WR+L NP   +SR+L  RYFP++  L A    S S  W+G + G ELL+ G+R 
Sbjct: 825  LLAKQAWRLLDNPSSLLSRLLKSRYFPHNSFLEAPHGHSPSLTWQGILHGRELLQSGLRW 884

Query: 61   NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
             +G GR I    DPWIP  T F  I Y  P   N VV   I  + QW+ + LD+    +D
Sbjct: 885  KVGEGRDINCATDPWIPGHTKFLPIHYAGP--PNAVVANLITEDRQWNKQLLDEFFSSID 944

Query: 121  VDVIKSLPIS-SHAPDRWIWHYDRRGEYSVKSGYK--LCIRDGREASSSDSGVEYSWWIN 180
            VD I ++P+S   A D  IWHY   G Y V SGY     + D   +S+S+S    +WW  
Sbjct: 945  VDRILTIPLSYFSANDHLIWHYHSSGIYKVHSGYHHLASLEDSDLSSTSNSST--NWWKY 1004

Query: 181  LWKLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQ 240
             WKL++P K+K F WR F++++P   +L+  H+  +  C +C++  E+  HALF C  A+
Sbjct: 1005 FWKLQLPQKIKIFAWRVFHDALPVATSLVRRHIITDSTCSVCRQAWESIGHALFGCKYAK 1064

Query: 241  EVWKTRFPTGFGIGFD---PMEIKDKWMWLSRANGPL-LERICVGAWAIWNDRNNLVHNR 300
             VW+    TGF   ++    M   D  ++LS  +  L +E I    W+IW +RN ++H +
Sbjct: 1065 AVWRA---TGFHFDWNAAVQMNKGDYLVYLSTIHSKLEMESIICILWSIWTERNRIIHGK 1124

Query: 301  PIPSVNLRCEWINSYLEEYWQANPKGNPCVQTEED------ISNIIYNGED---FILHTD 360
               S  L   +  +YL  Y  A  K  P   T         + +  +   D     ++ D
Sbjct: 1125 KAKSTVLLASFATNYLSNYHHAQAKYRPPEATANQNPPPAPVPDSPWRPPDPGSLKMNVD 1184

Query: 361  AAFMGGSCGSGVGLVLRNKLGQLQAAQS 373
            AA    +   G+G V+RN  G + AA S
Sbjct: 1185 AAINASTNQMGIGAVIRNCQGAVIAAFS 1205

BLAST of Lcy08g013350 vs. NCBI nr
Match: XP_030509050.1 (uncharacterized protein LOC115723712 [Cannabis sativa])

HSP 1 Score: 229.2 bits (583), Expect = 6.0e-56
Identity = 119/322 (36.96%), Postives = 175/322 (54.35%), Query Frame = 0

Query: 1    MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
            ++AKQ WR+L +P   I+RVL  RYF   + L AT+ +  S  WK  VWG ELL KG+R 
Sbjct: 878  LLAKQAWRILEDPSSLIARVLKSRYFATGVFLEATAGAQPSLTWKSIVWGKELLLKGLRW 937

Query: 61   NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
             +G+G  +     PWIP P+ FK + + SP   +V V +FI H+ QWD+ KL Q     D
Sbjct: 938  RIGSGADVNCVSQPWIPGPSNFKPLLFLSPD-RDVKVADFITHSRQWDVPKLQQFFTPPD 997

Query: 121  VDVIKSLPISSH-APDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
            VD I S+P+S     D  +WHY   G Y+VKSGYKL   D  ++  + S    +WW   W
Sbjct: 998  VDRILSIPLSLFPCEDVLLWHYSSAGFYTVKSGYKLA-SDVPDSQPNSSHTTETWWQYFW 1057

Query: 181  KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
             L++PSK++ F WR+++ ++PT   L   H+  +  CP+C+   ET +HA F CNRA++V
Sbjct: 1058 GLKLPSKIRIFAWRAYHEALPTAAILQYRHISSSPQCPLCQVHLETINHAFFWCNRAKQV 1117

Query: 241  WKTRFPTGFGIGFDPMEIKDKWMWL-SRANGPLLERICVGAWAIWNDRNNLVHNRPIPSV 300
            WK    +            D  +++ S+     +E      W IW  RN   H++   S 
Sbjct: 1118 WKNWDESINWSLSMTCSFSDFLVYVSSQLQAEKVELFLTTLWCIWTARNAETHSKSPKSA 1177

Query: 301  NLRCEWINSYLEEYWQANPKGN 321
                ++ +SYL E+ +A  K N
Sbjct: 1178 LQIFQFASSYLAEFRKAQAKTN 1197

BLAST of Lcy08g013350 vs. NCBI nr
Match: XP_030479077.1 (uncharacterized protein LOC115696311 [Cannabis sativa])

HSP 1 Score: 228.8 bits (582), Expect = 7.9e-56
Identity = 117/332 (35.24%), Postives = 178/332 (53.61%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++AKQ W++L +P   ++RVL  RYF N   LNA+  S  S  W+    G +LL KG+R 
Sbjct: 670 LLAKQAWKILEDPHSLLTRVLKARYFKNWNFLNASKDSLPSLTWQSICDGRDLLIKGLRW 729

Query: 61  NMGNGRSIYMYRDPWIPRPTTFKVISYPSPTMENVVVVEFIKHNGQWDLEKLDQHLDRVD 120
            +GNG S+    DPW+P  T+F+  ++      +  +  +I H+ QWDL  L QH  ++D
Sbjct: 730 KIGNGNSVKCASDPWLPGNTSFRPYTFCGDV--DFSIEHYITHDRQWDLNLLRQHFGQID 789

Query: 121 VDVIKSLPISSHA-PDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVEYSWWINLW 180
           +D I SL IS ++  DR IWH+   G Y+VKSGY L ++  +   SS S + + WW  LW
Sbjct: 790 IDRILSLSISPNSREDRLIWHHSDFGSYTVKSGYHLAVQLDKINESSTSDLNHGWWNRLW 849

Query: 181 KLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEV 240
            L++P KVK F WR  N+++PT VNL++  + V   C +C    E++ HALF C RAQ+V
Sbjct: 850 ALKLPKKVKIFAWRVINDALPTQVNLMHRKISVVASCSLCNCPRESSGHALFFCTRAQQV 909

Query: 241 WKTRFPTGFGIGFDPMEIKDKWMWLSRANG-------------PLLERICVGAWAIWNDR 300
           W           F P  +      +SR NG              +L +I    W IW +R
Sbjct: 910 WSLAHV------FSPNPL------ISRMNGFEIFSSIATVKQNDVLAKILCLMWCIWTER 969

Query: 301 NNLVHNRPIPSVNLRCEWINSYLEEYWQANPK 319
           N  +H        + C +++ Y++++  A+ K
Sbjct: 970 NKEIHGSKPKPTGVICSFVDMYVQQFLLAHAK 987

BLAST of Lcy08g013350 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 3.4e-33
Identity = 103/393 (26.21%), Postives = 169/393 (43.00%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++ KQ+WR+L  P+  +++V   RYF  S  LNA   S  SF WK      E+L++G R 
Sbjct: 68  LLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGARA 127

Query: 61  NMGNGRSIYMYRDPWI-PRPTTFKVISYPSPTMENVVVVEFIK-------HNGQWDLEKL 120
            +GNG  I ++R  W+  +P +  +     P  E   V   +K          +W  + +
Sbjct: 128 VVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGREWRKDVI 187

Query: 121 DQHLDRVDVDVIKSL-PISSHAPDRWIWHYDRRGEYSVKSGYKLCIRDGREASSSDSGVE 180
           +     V+  +I  L P      D + W Y   G+Y+VKSGY +  +   + SS     E
Sbjct: 188 EMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSSPQEVSE 247

Query: 181 YSW---WINLWKLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDH 240
            S    +  +WK +   K++HF+W+  +NS+P    L   H+     C  C    ET +H
Sbjct: 248 PSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSCKETVNH 307

Query: 241 ALFLCNRAQEVW---KTRFPTGFGIGFDPMEIKDKWMWLSRANGPLLER----ICVGAWA 300
            LF C  A+  W       P G G   D + +   W++      P  E+    +    W 
Sbjct: 308 LLFKCTFARLTWAISSIPIPLG-GEWADSIYVNLYWVFNLGNGNPQWEKASQLVPWLLWR 367

Query: 301 IWNDRNNLVHNRPIPSVNLRCEWINSYLEEYWQANPKGNPCVQTEEDISNIIYN------ 360
           +W +RN LV      +           LEE W+   +   C  T+  ++           
Sbjct: 368 LWKNRNELVFRGREFNAQEVLRRAEDDLEE-WRIRTEAESC-GTKPQVNRSSCGRWRPPP 427

Query: 361 GEDFILHTDAAFMGGSCGSGVGLVLRNKLGQLQ 369
            +    +TDA +   +   G+G VLRN+ G+++
Sbjct: 428 HQWVKCNTDATWNRDNERCGIGWVLRNEKGEVK 457

BLAST of Lcy08g013350 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 6.3e-27
Identity = 71/229 (31.00%), Postives = 113/229 (49.34%), Query Frame = 0

Query: 24  RYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRKNMGNGRSIYMYRDPWI----PRP 83
           RYF +  +L+A  R   S+ W   + G+ LLKKG R  +G+G++I +  D  +    PRP
Sbjct: 4   RYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHPPRP 63

Query: 84  TTFKVISYPSPTMENVVVVEFIKHNGQ---WDLEKLDQHLDRVDVDVIKSLPIS-SHAPD 143
                      T + + +    +  G    WD  K+ Q +D+ D   I  + ++ S  PD
Sbjct: 64  LN------TEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPD 123

Query: 144 RWIWHYDRRGEYSVKSGYKLCIRD---GREASSSDSGVEYSWWINLWKLRVPSKVKHFVW 203
           + IW+Y+  GEY+V+SGY L   D      A +   G        +W L +  K+KHF+W
Sbjct: 124 KIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHG-SIDLKTRIWNLPIMPKLKHFLW 183

Query: 204 RSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQEVWK 242
           R+ + ++ T   L    + ++  CP C  ENE+ +HALF C  A   W+
Sbjct: 184 RALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWR 225

BLAST of Lcy08g013350 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.7e-11
Identity = 34/118 (28.81%), Postives = 57/118 (48.31%), Query Frame = 0

Query: 178 LWKLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQ 237
           +WKL+   K+KHF+W+  + ++ T  NL   H+  +  C  C +E+ET+ H  F C  AQ
Sbjct: 18  IWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYAQ 77

Query: 238 EVWKTRFPTGFGIGFDPMEIKDKWMWLSRA----NGPLLERICVG-AWAIWNDRNNLV 291
           +VW+        +    + ++ K   L  +      P L  + +   W +W  RN LV
Sbjct: 78  QVWRASGIPHQELRTTGITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSRNQLV 135

BLAST of Lcy08g013350 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 2.7e-09
Identity = 23/65 (35.38%), Postives = 36/65 (55.38%), Query Frame = 0

Query: 178 LWKLRVPSKVKHFVWRSFNNSIPTMVNLLNHHVPVNGVCPMCKEENETTDHALFLCNRAQ 237
           +WKL V  K+KHF+WR    ++ T   L + ++  + +C  C  E ET  H +F C   Q
Sbjct: 37  IWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQ 96

Query: 238 EVWKT 243
            VW++
Sbjct: 97  SVWRS 101

BLAST of Lcy08g013350 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.9e-09
Identity = 25/76 (32.89%), Postives = 47/76 (61.84%), Query Frame = 0

Query: 1   MIAKQVWRVLCNPQLKISRVLCGRYFPNSMVLNATSRSSFSFFWKGFVWGMELLKKGIRK 60
           ++AKQ +R++  P   +SR+L  RYFP+S ++  +  +  S+ W+  + G ELL +G+ +
Sbjct: 69  LLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLR 128

Query: 61  NMGNGRSIYMYRDPWI 77
            +G+G    ++ D WI
Sbjct: 129 TIGDGIHTKVWLDRWI 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C2F63.7e-1625.48Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P932958.3e-0832.89Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DX304.5e-7337.27uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803Q1058.2e-5935.18Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QQT25.3e-5834.72Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QGT27.0e-5834.65Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803Q1853.5e-5732.89Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022158377.19.3e-7337.27uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_030479133.11.4e-5734.65uncharacterized protein LOC115696372 [Cannabis sativa][more]
XP_030483481.12.5e-5734.79uncharacterized protein LOC115700065 [Cannabis sativa][more]
XP_030509050.16.0e-5636.96uncharacterized protein LOC115723712 [Cannabis sativa][more]
XP_030479077.17.9e-5635.24uncharacterized protein LOC115696311 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
AT4G29090.13.4e-3326.21Ribonuclease H-like superfamily protein [more]
AT3G09510.16.3e-2731.00Ribonuclease H-like superfamily protein [more]
AT3G25270.13.7e-1128.81Ribonuclease H-like superfamily protein [more]
AT2G02650.12.7e-0935.38Ribonuclease H-like superfamily protein [more]
ATMG00310.15.9e-0932.89RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 147..240
e-value: 1.9E-20
score: 73.3
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 45..372
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 45..372

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g013350.1Lcy08g013350.1mRNA