Lcy08g013080 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g013080
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr08: 42682471 .. 42683036 (-)
RNA-Seq ExpressionLcy08g013080
SyntenyLcy08g013080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAAAACATCTCAAGAATATACACAGATTTCCTTCTTCCTCTCTGTCTGCTTTTACTTGGTTGAAGAAAACCCCCTTTGTTCAAGATGTGGATGTGTTGGAGGCGATGGCTGTCCGAGATAGCCTTCTGTTGGCGAAAGATGGTGGTTTCTTGCAGCTGGAGGTGGAATCTGACTCGGCCAGTGTGGTATCGCTGCTCCGATCGAACGCTTGTGAAAAATCTGAGGTGGGCAAGATTGTGGACGAAATTCGCCAAATTGCCAAGGAATTCCAGTCGTGCTCCTTTTCATGGTGCTCCAGGTCGAGGAATGTGGTGGCCCATAAAGCCGCAAGAGCGGCGCTGTTGGGTGAGCTGAACGGTGTATGGATTGAAGATGTGTCGGCGCCGCTGCGTCATGCTCTTGAAGCTGAGCGTTTAGACAGGTCGAGGGTTGTTGAGTTGCGTTGTCTGTAGCCCTTTTCTGTCGGTTTGCTTTGTTCAAAAAAAAAAAAACTTTTTATTCATTTTTCTCTCTTTTTTTTTTTTTTATCTCTAACTATTTTTTTTCTCATTGATTGTTTGTTGT

mRNA sequence

GTGAAAACATCTCAAGAATATACACAGATTTCCTTCTTCCTCTCTGTCTGCTTTTACTTGGTTGAAGAAAACCCCCTTTGTTCAAGATGTGGATGTGTTGGAGGCGATGGCTGTCCGAGATAGCCTTCTGTTGGCGAAAGATGGTGGTTTCTTGCAGCTGGAGGTGGAATCTGACTCGGCCAGTGTGGTATCGCTGCTCCGATCGAACGCTTGTGAAAAATCTGAGGTGGGCAAGATTGTGGACGAAATTCGCCAAATTGCCAAGGAATTCCAGTCGTGCTCCTTTTCATGGTGCTCCAGGTCGAGGAATGTGGTGGCCCATAAAGCCGCAAGAGCGGCGCTGTTGGGTGAGCTGAACGGTGTATGGATTGAAGATGTGTCGGCGCCGCTGCGTCATGCTCTTGAAGCTGAGCGTTTAGACAGGTCGAGGGTTGTTGAGTTGCGTTGTCTGTAGCCCTTTTCTGTCGGTTTGCTTTGTTCAAAAAAAAAAAAACTTTTTATTCATTTTTCTCTCTTTTTTTTTTTTTTATCTCTAACTATTTTTTTTCTCATTGATTGTTTGTTGT

Coding sequence (CDS)

ATGGCTGTCCGAGATAGCCTTCTGTTGGCGAAAGATGGTGGTTTCTTGCAGCTGGAGGTGGAATCTGACTCGGCCAGTGTGGTATCGCTGCTCCGATCGAACGCTTGTGAAAAATCTGAGGTGGGCAAGATTGTGGACGAAATTCGCCAAATTGCCAAGGAATTCCAGTCGTGCTCCTTTTCATGGTGCTCCAGGTCGAGGAATGTGGTGGCCCATAAAGCCGCAAGAGCGGCGCTGTTGGGTGAGCTGAACGGTGTATGGATTGAAGATGTGTCGGCGCCGCTGCGTCATGCTCTTGAAGCTGAGCGTTTAGACAGGTCGAGGGTTGTTGAGTTGCGTTGTCTGTAG

Protein sequence

MAVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFSWCSRSRNVVAHKAARAALLGELNGVWIEDVSAPLRHALEAERLDRSRVVELRCL
Homology
BLAST of Lcy08g013080 vs. ExPASy TrEMBL
Match: B9T5J0 (RNase H domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_0157780 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.4e-08
Identity = 40/90 (44.44%), Postives = 57/90 (63.33%), Query Frame = 0

Query: 2   AVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFS 61
           AVR  LLLAKD GF  L+VESD+A  +S+L+ N    +E+G I+D+I+ I ++F S  +S
Sbjct: 38  AVRWGLLLAKDAGFRVLDVESDNAHSISMLKGNQENFTELGTIIDDIKFICRDFSSIVWS 97

Query: 62  WCSRSRNVVAHKAARAALLGELNGVWIEDV 92
             +R  N VAH  A +      + VWIE+V
Sbjct: 98  HVNRVCNKVAHMVA-SLTNSTTDRVWIENV 126

BLAST of Lcy08g013080 vs. ExPASy TrEMBL
Match: A0A2K2D3A0 (RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3g45155v3 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-07
Identity = 32/88 (36.36%), Postives = 56/88 (63.64%), Query Frame = 0

Query: 1   MAVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSF 60
           +A+RD+  LAK+  F ++ +E+D A +V L R+ + ++S VG I+DEI + ++ F SC  
Sbjct: 227 LALRDACCLAKEKAFQRVILETDCAELVRLWRARSSQRSVVGPILDEIIEFSQSFISCEI 286

Query: 61  SWCSRSRNVVAHKAARAALLGELNGVWI 89
            +  R  N VAH+ AR+A+   ++  W+
Sbjct: 287 VFVRREANFVAHECARSAVSNGVSSEWV 314

BLAST of Lcy08g013080 vs. ExPASy TrEMBL
Match: A0A2N9EYC3 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7737 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-07
Identity = 35/98 (35.71%), Postives = 53/98 (54.08%), Query Frame = 0

Query: 2    AVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFS 61
            A R ++  AKD GF+ +++E DS S+V  +       +  G I+++IRQIA+  QS  F 
Sbjct: 1525 AARSAIQFAKDSGFMNIDLEGDSTSIVEAILLTTPCSTIYGNIIEDIRQIARGLQSVQFL 1584

Query: 62   WCSRSRNVVAHKAARAALLGELNGVWIEDVSAPLRHAL 100
               R  NV+AH  A+ A + +    W+E V   L H L
Sbjct: 1585 HVKREGNVMAHLLAKRAGINKPFETWMETVPPELSHTL 1622

BLAST of Lcy08g013080 vs. ExPASy TrEMBL
Match: A0A2N9IYC8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57142 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.9e-07
Identity = 35/98 (35.71%), Postives = 53/98 (54.08%), Query Frame = 0

Query: 2    AVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFS 61
            A R ++  AKD GF+ +++E DS S+V  +       +  G I+++IRQIA+  QS  F 
Sbjct: 1568 AARSAIQFAKDLGFMNIDLEGDSTSIVEAILLTTPCSTIYGNIIEDIRQIARGLQSVQFL 1627

Query: 62   WCSRSRNVVAHKAARAALLGELNGVWIEDVSAPLRHAL 100
               R  NV+AH  A+ A + +    W+E V   L H L
Sbjct: 1628 HIKREGNVMAHLLAKRAGINKPFETWMESVPPELSHTL 1665

BLAST of Lcy08g013080 vs. ExPASy TrEMBL
Match: A0A2N9G0G1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24139 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 33/98 (33.67%), Postives = 52/98 (53.06%), Query Frame = 0

Query: 2    AVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFS 61
            A R ++  AKD GF  +++E DS S+V  ++      +  G ++++IR IA+  QS  F 
Sbjct: 1452 AARSAIQFAKDSGFTNIDLEGDSTSIVEAIQLTTPCSTIYGNLIEDIRHIARGLQSVQFL 1511

Query: 62   WCSRSRNVVAHKAARAALLGELNGVWIEDVSAPLRHAL 100
               R  NV+AH  A+ A + +    W+E V   L H L
Sbjct: 1512 HVKREGNVMAHLLAKRAGINKPFETWMESVPPELSHTL 1549

BLAST of Lcy08g013080 vs. NCBI nr
Match: XP_023881690.1 (uncharacterized protein LOC111994061 [Quercus suber])

HSP 1 Score: 77.8 bits (190), Expect = 6.8e-11
Identity = 46/113 (40.71%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 1   MAVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSF 60
           +A R ++  A D GF+ L +E D+A+V+  + S+  ++S +G I D+IR +A  F+SC+F
Sbjct: 51  LACRKAVEFAMDAGFMDLVIEGDNAAVMKAITSSRLDRSRLGHIYDDIRTLAARFRSCNF 110

Query: 61  SWCSRSRNVVAHKAARAA--LLGELNGVWIEDVSAPLRHALEAERLDRSRVVE 112
               RS N VAH  AR A  L+ EL  VW+E+   P   ALEA  LD S +++
Sbjct: 111 GCVKRSANAVAHSLARFASNLVDEL--VWLEEPPPP---ALEALYLDASFLID 158

BLAST of Lcy08g013080 vs. NCBI nr
Match: XP_023905314.1 (uncharacterized protein LOC112017083 [Quercus suber])

HSP 1 Score: 73.6 bits (179), Expect = 1.3e-09
Identity = 45/109 (41.28%), Postives = 66/109 (60.55%), Query Frame = 0

Query: 1   MAVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSF 60
           +A R ++  A D GF+ L +E D+A+V+  + S+  + S +G I D+IR +A  F+SC+F
Sbjct: 51  LACRKAVEFAMDAGFINLVIEGDNAAVMKAITSSRLDGSRLGHIYDDIRTLAAGFRSCNF 110

Query: 61  SWCSRSRNVVAHKAARAA--LLGELNGVWIEDVSAPLRHALEAERLDRS 108
               RS NVVAH  AR A  ++ EL  VW+++   P   ALEA  LD S
Sbjct: 111 GCVKRSANVVAHSLARFANHIVDEL--VWLKESPPP---ALEALYLDAS 154

BLAST of Lcy08g013080 vs. NCBI nr
Match: KAG2565207.1 (hypothetical protein PVAP13_7NG002334 [Panicum virgatum])

HSP 1 Score: 68.6 bits (166), Expect = 4.1e-08
Identity = 35/98 (35.71%), Postives = 59/98 (60.20%), Query Frame = 0

Query: 2   AVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFS 61
           A+RD LLLA+  G  ++++++D   VV  +       + +  I DE  ++ ++F + S S
Sbjct: 365 AMRDGLLLAQQIGSSRVQIQADCMEVVDTMLEGGYSATALAAIYDECSKLWEDFDAISIS 424

Query: 62  WCSRSRNVVAHKAARAALLGELNGVWIEDVSAPLRHAL 100
            C+R  NVVAH+ AR A+L + + VWI+D  A +R +L
Sbjct: 425 HCNRESNVVAHELARQAMLEKNSCVWIDDPPASIRQSL 462

BLAST of Lcy08g013080 vs. NCBI nr
Match: EEF28878.1 (conserved hypothetical protein [Ricinus communis])

HSP 1 Score: 67.8 bits (164), Expect = 7.1e-08
Identity = 40/90 (44.44%), Postives = 57/90 (63.33%), Query Frame = 0

Query: 2   AVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSFS 61
           AVR  LLLAKD GF  L+VESD+A  +S+L+ N    +E+G I+D+I+ I ++F S  +S
Sbjct: 38  AVRWGLLLAKDAGFRVLDVESDNAHSISMLKGNQENFTELGTIIDDIKFICRDFSSIVWS 97

Query: 62  WCSRSRNVVAHKAARAALLGELNGVWIEDV 92
             +R  N VAH  A +      + VWIE+V
Sbjct: 98  HVNRVCNKVAHMVA-SLTNSTTDRVWIENV 126

BLAST of Lcy08g013080 vs. NCBI nr
Match: XP_024317835.1 (uncharacterized protein LOC112271862 [Brachypodium distachyon] >XP_024317836.1 uncharacterized protein LOC112271862 [Brachypodium distachyon] >PNT68773.1 hypothetical protein BRADI_3g45155v3 [Brachypodium distachyon] >PNT68774.1 hypothetical protein BRADI_3g45155v3 [Brachypodium distachyon])

HSP 1 Score: 65.9 bits (159), Expect = 2.7e-07
Identity = 32/88 (36.36%), Postives = 56/88 (63.64%), Query Frame = 0

Query: 1   MAVRDSLLLAKDGGFLQLEVESDSASVVSLLRSNACEKSEVGKIVDEIRQIAKEFQSCSF 60
           +A+RD+  LAK+  F ++ +E+D A +V L R+ + ++S VG I+DEI + ++ F SC  
Sbjct: 227 LALRDACCLAKEKAFQRVILETDCAELVRLWRARSSQRSVVGPILDEIIEFSQSFISCEI 286

Query: 61  SWCSRSRNVVAHKAARAALLGELNGVWI 89
            +  R  N VAH+ AR+A+   ++  W+
Sbjct: 287 VFVRREANFVAHECARSAVSNGVSSEWV 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
B9T5J03.4e-0844.44RNase H domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_0157780 PE... [more]
A0A2K2D3A01.3e-0736.36RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3... [more]
A0A2N9EYC31.3e-0735.71Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9IYC82.9e-0735.71Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9G0G16.4e-0733.67Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24139 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_023881690.16.8e-1140.71uncharacterized protein LOC111994061 [Quercus suber][more]
XP_023905314.11.3e-0941.28uncharacterized protein LOC112017083 [Quercus suber][more]
KAG2565207.14.1e-0835.71hypothetical protein PVAP13_7NG002334 [Panicum virgatum][more]
EEF28878.17.1e-0844.44conserved hypothetical protein [Ricinus communis][more]
XP_024317835.12.7e-0736.36uncharacterized protein LOC112271862 [Brachypodium distachyon] >XP_024317836.1 u... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..82
e-value: 9.2E-11
score: 43.8
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 2..78
e-value: 1.4E-17
score: 63.6
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 1..76
e-value: 9.77757E-16
score: 65.7984
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 1..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g013080.1Lcy08g013080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity