Lcy08g010220 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g010220
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNA-directed DNA polymerase (reverse transcriptase)-related family protein
LocationChr08: 30322248 .. 30323657 (-)
RNA-Seq ExpressionLcy08g010220
SyntenyLcy08g010220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTAGTCCAAATACTAATGAGGATCTAGTCCAGAAGGTTAAGGATGTTTTGCAGGTTCATTATACAGACAGCTTGGGGCAATATCTTGGACTACCATCCCAAAGCGGCACAAATAAGAGGGCGATTTTCAACAATATAAAGGACCGTGTATGGCGAGCGCTACAAGGATGGAAGGGGAAATTTTTCTCGGCAGCTGGCAGGGAGACGTTTATAAAAGCAGTGGCGCAGGCGATTCCTAACTACCCGATGGGGTGTTTCAAACTCCCTTTAACCTTAACTAATGAGCTATACTCTCTCTGTGCTAGGTTCTGGTGGGGGTCGGAGGAGGCAGGAAAAAAGATCCATTGGAGAAGCTGGGAAAAGATGTGCATTCATAAGGCTCAAGGAGGCATGGGTTTCAAAGATTTATCCATTTTCAATCAGGCGATGCTAGCAAAGCAAAGTTGGAGAATCATTCGGAATCCGGAGAGTTTGCTAGCAAGAGTGCTTAGAGGGCGATATTTCAAAACTGGCCATTTTCTAAAGGCGGGAGCTGGTTATAATCCCTCTTACACATGGAGGAGCATCCTTTGGGGCAGAGAGCTGTTTAAAAAAGGGTACCGTTGGCGTATTGGGAGAGGCGTTAATGTAGAGGCTGCTTCGGACCCATGGATACCAAGGGAAGGATCGTGCAAACCAATTCCAAGGAATATCGAGGCTTATAAGTACACAGTAGCGCAACTAAAGTACCCTTCTGGGTATTGGCGGGAGGGTTTGGTTAGAGATTTGTTTTTGGAAGAGGATGCAAAAGCTATCCTAAATATTCCTATCAGTCCTAGCCAAAATTTGGATGAAATCATATGGAATCTTGACCCAAAAGGCCTCTTCACGGTTAGAAGTGCATACAGATTGGGTCGCCAAGGAAAAAACGCACAAAACGCATCTACATCGAAAAATTCCTACAAAGAAAACATTTGGAAGCGGTTTTGGAAATCTAAAACTCCCCCAAAAGTCAAAGTTTGTGGTTGGAAGATATACAATGATATTATTCGGACATGTTCACACTTGATTAATAGGGGTTTAGATGTTAATCCTCTATGTTTACTGTGCAGGTCCAAAACAGAGACGACCAAGCATCTTTTTTGGGAGTGCAAAATAATGAGAGGTATGTGGACCAAATTTCTTTCGTATTCTAATAGTTTGTCTGGTGTTGATAGGTGCGGATGGACAATACCAAATTATTTTGAAGGACTATGGAAGGAGAGTAGCAGGGAAGATACAGAAGACAGAAGAATAGAGAAAAGCCTTATAATCTGTTGGCAAATCTGGAACTTTCGGAATGAAATTCTTCATAATAACCAGCAGCCAAGCCAAAATAAGCTTTCACAACAAATTCGAAATGCTTTTATCAAGTTCCAGAGAATATAA

mRNA sequence

ATGACTAGTCCAAATACTAATGAGGATCTAGTCCAGAAGGTTAAGGATGTTTTGCAGGTTCATTATACAGACAGCTTGGGGCAATATCTTGGACTACCATCCCAAAGCGGCACAAATAAGAGGGCGATTTTCAACAATATAAAGGACCGTGTATGGCGAGCGCTACAAGGATGGAAGGGGAAATTTTTCTCGGCAGCTGGCAGGGAGACGTTTATAAAAGCAGTGGCGCAGGCGATTCCTAACTACCCGATGGGGTGTTTCAAACTCCCTTTAACCTTAACTAATGAGCTATACTCTCTCTGTGCTAGGTTCTGGTGGGGGTCGGAGGAGGCAGGAAAAAAGATCCATTGGAGAAGCTGGGAAAAGATGTGCATTCATAAGGCTCAAGGAGGCATGGGTTTCAAAGATTTATCCATTTTCAATCAGGCGATGCTAGCAAAGCAAAGTTGGAGAATCATTCGGAATCCGGAGAGTTTGCTAGCAAGAGTGCTTAGAGGGCGATATTTCAAAACTGGCCATTTTCTAAAGGCGGGAGCTGGTTATAATCCCTCTTACACATGGAGGAGCATCCTTTGGGGCAGAGAGCTGTTTAAAAAAGGGTACCGTTGGCGTATTGGGAGAGGCGTTAATGTAGAGGCTGCTTCGGACCCATGGATACCAAGGGAAGGATCGTGCAAACCAATTCCAAGGAATATCGAGGCTTATAAGTACACAGTAGCGCAACTAAAGTACCCTTCTGGGTATTGGCGGGAGGGTTTGGTTAGAGATTTGTTTTTGGAAGAGGATGCAAAAGCTATCCTAAATATTCCTATCAGTCCTAGCCAAAATTTGGATGAAATCATATGGAATCTTGACCCAAAAGGCCTCTTCACGGTTAGAAGTGCATACAGATTGGGTCGCCAAGGAAAAAACGCACAAAACGCATCTACATCGAAAAATTCCTACAAAGAAAACATTTGGAAGCGGTTTTGGAAATCTAAAACTCCCCCAAAAGTCAAAGTTTGTGGTTGGAAGATATACAATGATATTATTCGGACATGTTCACACTTGATTAATAGGGGTTTAGATGTTAATCCTCTATGTTTACTGTGCAGGTCCAAAACAGAGACGACCAAGCATCTTTTTTGGGAGTGCAAAATAATGAGAGGTATGTGGACCAAATTTCTTTCGTATTCTAATAGTTTGTCTGGTGTTGATAGGTGCGGATGGACAATACCAAATTATTTTGAAGGACTATGGAAGGAGAGTAGCAGGGAAGATACAGAAGACAGAAGAATAGAGAAAAGCCTTATAATCTGTTGGCAAATCTGGAACTTTCGGAATGAAATTCTTCATAATAACCAGCAGCCAAGCCAAAATAAGCTTTCACAACAAATTCGAAATGCTTTTATCAAGTTCCAGAGAATATAA

Coding sequence (CDS)

ATGACTAGTCCAAATACTAATGAGGATCTAGTCCAGAAGGTTAAGGATGTTTTGCAGGTTCATTATACAGACAGCTTGGGGCAATATCTTGGACTACCATCCCAAAGCGGCACAAATAAGAGGGCGATTTTCAACAATATAAAGGACCGTGTATGGCGAGCGCTACAAGGATGGAAGGGGAAATTTTTCTCGGCAGCTGGCAGGGAGACGTTTATAAAAGCAGTGGCGCAGGCGATTCCTAACTACCCGATGGGGTGTTTCAAACTCCCTTTAACCTTAACTAATGAGCTATACTCTCTCTGTGCTAGGTTCTGGTGGGGGTCGGAGGAGGCAGGAAAAAAGATCCATTGGAGAAGCTGGGAAAAGATGTGCATTCATAAGGCTCAAGGAGGCATGGGTTTCAAAGATTTATCCATTTTCAATCAGGCGATGCTAGCAAAGCAAAGTTGGAGAATCATTCGGAATCCGGAGAGTTTGCTAGCAAGAGTGCTTAGAGGGCGATATTTCAAAACTGGCCATTTTCTAAAGGCGGGAGCTGGTTATAATCCCTCTTACACATGGAGGAGCATCCTTTGGGGCAGAGAGCTGTTTAAAAAAGGGTACCGTTGGCGTATTGGGAGAGGCGTTAATGTAGAGGCTGCTTCGGACCCATGGATACCAAGGGAAGGATCGTGCAAACCAATTCCAAGGAATATCGAGGCTTATAAGTACACAGTAGCGCAACTAAAGTACCCTTCTGGGTATTGGCGGGAGGGTTTGGTTAGAGATTTGTTTTTGGAAGAGGATGCAAAAGCTATCCTAAATATTCCTATCAGTCCTAGCCAAAATTTGGATGAAATCATATGGAATCTTGACCCAAAAGGCCTCTTCACGGTTAGAAGTGCATACAGATTGGGTCGCCAAGGAAAAAACGCACAAAACGCATCTACATCGAAAAATTCCTACAAAGAAAACATTTGGAAGCGGTTTTGGAAATCTAAAACTCCCCCAAAAGTCAAAGTTTGTGGTTGGAAGATATACAATGATATTATTCGGACATGTTCACACTTGATTAATAGGGGTTTAGATGTTAATCCTCTATGTTTACTGTGCAGGTCCAAAACAGAGACGACCAAGCATCTTTTTTGGGAGTGCAAAATAATGAGAGGTATGTGGACCAAATTTCTTTCGTATTCTAATAGTTTGTCTGGTGTTGATAGGTGCGGATGGACAATACCAAATTATTTTGAAGGACTATGGAAGGAGAGTAGCAGGGAAGATACAGAAGACAGAAGAATAGAGAAAAGCCTTATAATCTGTTGGCAAATCTGGAACTTTCGGAATGAAATTCTTCATAATAACCAGCAGCCAAGCCAAAATAAGCTTTCACAACAAATTCGAAATGCTTTTATCAAGTTCCAGAGAATATAA

Protein sequence

MTSPNTNEDLVQKVKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIKAVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGRELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREGLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSKNSYKENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTETTKHLFWECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIEKSLIICWQIWNFRNEILHNNQQPSQNKLSQQIRNAFIKFQRI
Homology
BLAST of Lcy08g010220 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.7e-34
Identity = 108/412 (26.21%), Postives = 167/412 (40.53%), Query Frame = 0

Query: 44  FNNIKDRVWRALQGWKGKFFSAAGRETFIKAVAQAIPNYPMGCFKLPLTLTNELYSLCAR 103
           F  I +RV   + GW+ K  S AGR T  KAV  ++P + M    LP ++ N L  L   
Sbjct: 13  FGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRT 72

Query: 104 FWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKDLSIFNQAMLAKQSWRIIRNPESLLARV 163
           F WGS    KK H   W K+C  K +GG+G +     N+A+++K  WR+++   SL   V
Sbjct: 73  FLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLV 132

Query: 164 LRGRY----FKTGHFLKAGAGYNPSYTWRSILWG-RELFKKGYRWRIGRGVNVEAASDPW 223
           L+ +Y     +   +L     +  S TWRSI  G R++   G  W  G G  +   +D W
Sbjct: 133 LQKKYHVGEIRDSRWLIPKGSW--SSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRW 192

Query: 224 IPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREGLVRDLFLEEDAKAILNIPIS------ 283
           +    S KP+       + T          W  G   D F + D     N  +       
Sbjct: 193 V----SGKPLLELDNGERPTDCDTVVAKDLWIPGRGWD-FAKIDPYTTNNTRLELRAVVL 252

Query: 284 --PSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSKNSYKENIWKRFWKSKTPP 343
              +   D + W     G F+VRSAY +                   + +   WK + P 
Sbjct: 253 DLVTGARDRLSWKFSQDGQFSVRSAYEM-------LTVDEVPRPNMASFFNCLWKVRVPE 312

Query: 344 KVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTETTKHLFWECKIMRGMWTKFLS 403
           +VK   W + N  + T      R L  + +C +C+   E+  H+  +C    G+W + + 
Sbjct: 313 RVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVP 372

Query: 404 YSNSLSGVDRCGWTIPNYFEGLWKE-SSREDTEDRRIEKSL-IICWQIWNFR 441
                    + G+   + FE L+     R   ED        +I W  W +R
Sbjct: 373 QRR------QQGFFSKSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWR 404

BLAST of Lcy08g010220 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.4e-34
Identity = 70/152 (46.05%), Postives = 92/152 (60.53%), Query Frame = 0

Query: 78  AIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKA-QGGMGFKD 137
           A+P Y M CF+L   L  +L S    FWW S E  +KI W +W+K+C  K   GG+GF+D
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 138 LSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGREL 197
           L  FNQA+LAKQS+RII  P +LL+R+LR RYF     ++   G  PSY WRSI+ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 198 FKKGYRWRIGRGVNVEAASDPWIPREGSCKPI 229
             +G    IG G++ +   D WI  E    P+
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPLPPL 153

BLAST of Lcy08g010220 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 2.7e-96
Identity = 181/449 (40.31%), Postives = 254/449 (56.57%), Query Frame = 0

Query: 14  VKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIK 73
           ++++L V+  +   QYLGLP+    N+R  FN IKDRVW+ LQGWK K FS  G+E  IK
Sbjct: 528 IQNILSVNMVECQLQYLGLPTFMPRNRRMHFNYIKDRVWKHLQGWKAKLFSIGGKEVLIK 587

Query: 74  AVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMG 133
           AVAQAIP Y M CF+LP  L  E + + ARFWWGS +  KKIHW +W  + + K +GGMG
Sbjct: 588 AVAQAIPCYTMSCFRLPKRLIREFHHITARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMG 647

Query: 134 FKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWG 193
           F+DL +FN+A+LAKQ WRI+ +P S+L+RVL+GRYFK   F++A    NPSY WRSILWG
Sbjct: 648 FRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWG 707

Query: 194 RELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPI--PRNIEAYKYTVAQLKYPSGYWRE 253
           R+L KKG RWRIG G +V    D W+P + + K +  PR +       + + +  G W+ 
Sbjct: 708 RDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPR-LPLVSRVSSLVDHEEGGWQG 767

Query: 254 GLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTS 313
            +VRD F  ++AK IL+IPI      D +IWN +  G+++VRS Y++         A +S
Sbjct: 768 DVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSS 827

Query: 314 KNSYKENI-WKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTET 373
            +S +    W  FWK   P K+KV  W++  D + T  +L  RG+++   C  C    E 
Sbjct: 828 SSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGED 887

Query: 374 TKHLFWECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIEKSL 433
           + HLFW CK    +W       NS  G              L    S E       E+  
Sbjct: 888 SIHLFWICKFAEALWI------NSKFG---------KLSPFLILRESHESLSKADFEELC 947

Query: 434 IICWQIWNFRNEILHNNQQPSQNKLSQQI 460
           ++ W +WN RN    N+   +  K+  ++
Sbjct: 948 VVIWGLWNQRNARAFNDSTKTVFKIGMEL 960

BLAST of Lcy08g010220 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 6.3e-93
Identity = 168/452 (37.17%), Postives = 245/452 (54.20%), Query Frame = 0

Query: 17  VLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIKAVA 76
           +L V   ++ G+YLGLPS  G NK+   + IK++VW  ++GWK   FS AG+E  IK + 
Sbjct: 85  ILGVREVENHGKYLGLPSFVGRNKKEFLDEIKNKVWAKMKGWKTSMFSVAGKEVLIKVIV 144

Query: 77  QAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKD 136
           QAIP Y M CFKLP    + L+ + +RFWWGS +  KKIHW  W  +C  K +GG+GF+D
Sbjct: 145 QAIPTYTMSCFKLPKKTISSLHRMASRFWWGSLDKEKKIHWCKWRYLCRPKDKGGLGFRD 204

Query: 137 LSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGREL 196
           L +FNQA+LAKQ WR +R+P+ L +RVL+  YF     L+AG G N S+ WRS++WG++L
Sbjct: 205 LGMFNQALLAKQIWRCLRHPQLLCSRVLKASYFPRKGVLEAGCGANASFVWRSLVWGKKL 264

Query: 197 FKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREGLVRD 256
             KGYRWR+G G +V    DPW+PR  + K   +        V  LK   G W EG +R 
Sbjct: 265 ILKGYRWRVGNGESVRVLEDPWLPRPVTFKVYDQPSLPANLYVTDLKLADGQWDEGFIRS 324

Query: 257 LFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSKNSYK 316
           +F   D   IL IP S     D+I+W+    G ++V+S YR+       Q+ S   +  +
Sbjct: 325 IFNPTDVDLILGIPCSDWDFEDKILWHYSKYGEYSVKSGYRMAASFTTEQHQSNEHSIVQ 384

Query: 317 ENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKT-ETTKHLF 376
              WK+ W+ K PPKVK   WK+ ++ +    +L  RG+  + +C  C S   E+  H  
Sbjct: 385 --WWKKLWRLKIPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRCSSHVDESVAHAL 444

Query: 377 WECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIEKSLIICWQ 436
           WECK  +G W     Y +    +     T+       W        +  ++E  L++ W 
Sbjct: 445 WECKASKGYWRVSGLYDDLKQMLGEDNLTMLMRIAAEW--------DKEKLEFFLLVSWN 504

Query: 437 IWNFRNEILHNNQQPSQNKLSQQIRNAFIKFQ 468
           IWN RN ++H    P   ++ +   N    F+
Sbjct: 505 IWNVRNTVVHGGYHPKPEEMIEWCGNFLADFR 526

BLAST of Lcy08g010220 vs. ExPASy TrEMBL
Match: A0A803QEG9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 7.0e-92
Identity = 165/429 (38.46%), Postives = 240/429 (55.94%), Query Frame = 0

Query: 28   QYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIKAVAQAIPNYPMGCF 87
            +YLGLPS SG +KR +F+NIK+R+W+ +Q W  K FSA GRE  +KAV Q+IP Y M CF
Sbjct: 1259 KYLGLPSYSGRDKRELFSNIKERIWKLMQSWSEKLFSAGGREVLLKAVVQSIPTYAMSCF 1318

Query: 88   KLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKDLSIFNQAMLAK 147
            +LP+   N+L S+ A FWWG  E G KIHWRSW  +C  K +GGMGF+    FNQA+LAK
Sbjct: 1319 RLPVYFCNQLESMMANFWWGLNENGSKIHWRSWNLLCKTKGEGGMGFRSFIQFNQALLAK 1378

Query: 148  QSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGRELFKKGYRWRIGR 207
            Q+WRI+  P SLL+ +L+ +YF    FL+A  G++PS TW+ I WGREL   G RW+IG 
Sbjct: 1379 QAWRIVEYPNSLLSTLLKSKYFPHNSFLEASMGHSPSLTWQGIRWGRELLAHGLRWKIGE 1438

Query: 208  GVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREGLVRDLFLEEDAKAIL 267
            G  V +  DPW+P   S  PI  +       VA L      W   L++  F   D + IL
Sbjct: 1439 GRTVRSGFDPWLPGHSSFLPITYS-GPPNGVVANLITDERQWNIPLLQQYFCPLDIEKIL 1498

Query: 268  NIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSKNSYKENIWKRFWKSK 327
             +P+S     D++IW+    G FTV+SAY L    +N   +S+S ++ +   WK FW  +
Sbjct: 1499 TLPLSFFPTRDKLIWHHHSSGNFTVQSAYHLATSLENEDLSSSSTSAVQ--WWKHFWSLQ 1558

Query: 328  TPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTETTKHLFWECKIMRGMWTK 387
             P KVK+  W++ +D +   + L+ R +  +  C +C+   E+T H  + CK  + +W  
Sbjct: 1559 IPQKVKIFAWRVIHDALPVATSLVRRKIITDSTCSICKQAWESTGHALFGCKYAKAVWRS 1618

Query: 388  FLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIEKSLIICWQIWNFRNEILHNN 447
            F    N  + +         Y   ++ ++         +E+   I W IW  RN+++H  
Sbjct: 1619 FHFNFNWSAAISMKNGDYLMYLSSIYNKA--------EMEELFCIMWAIWGERNKVVHGK 1676

Query: 448  QQPSQNKLS 457
                 N L+
Sbjct: 1679 NARRANDLA 1676

BLAST of Lcy08g010220 vs. ExPASy TrEMBL
Match: A0A803NHG3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 9.1e-92
Identity = 168/439 (38.27%), Postives = 243/439 (55.35%), Query Frame = 0

Query: 13   KVKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFI 72
            ++ + L V   D+ G+YLGL S  G NK+ +F+ IK+RVW  L+GWKG  FS   +E  I
Sbjct: 1172 ELAEFLGVKVVDNHGKYLGLLSLVGRNKKQVFDVIKNRVWNRLRGWKGHMFSMGVKEVLI 1231

Query: 73   KAVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGM 132
            KA+ QAIP Y M C++L  +    ++ + ARFWWGS    KKIHW  WE +C  K +GG+
Sbjct: 1232 KAIVQAIPAYTMSCYRLTKSTITSIHRMAARFWWGSTAKKKKIHWCKWEYLCRPKEEGGL 1291

Query: 133  GFKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILW 192
            GF+DL +FNQA+LAKQ WR +R P SL ++VL+  YF     L A  G + S+ WRS++W
Sbjct: 1292 GFRDLELFNQALLAKQIWRCLRQPGSLCSKVLKASYFPHKSVLAAKCGAHASFVWRSLVW 1351

Query: 193  GRELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREG 252
            G+E+  KGYRWR+G G  V    DPW+PR  S K   +     +  V  L +PSG W E 
Sbjct: 1352 GKEIILKGYRWRVGNGQQVRVLEDPWLPRPTSFKVYDKPPFPQQLCVVDLTHPSGVWDES 1411

Query: 253  LVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSK 312
             +R  F  EDA+ IL +P       D+++W+    G +TVRS YR+  + + ++  +T +
Sbjct: 1412 FIRANFNREDAEMILRLPPLHEDLEDKVMWHYSRNGEYTVRSGYRMAAEIRKSE--ATLE 1471

Query: 313  NSYKENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKT-ETT 372
                ++ W++ WK K PPKVK   WK+ N  + T S+L+ R +  +P C  C +   E  
Sbjct: 1472 GQLMKDWWRKLWKLKLPPKVKHFAWKLANSWLPTHSNLVTRKVMTDPTCHRCSNGVRENI 1531

Query: 373  KHLFWECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIEKSLI 432
             H  W C   +G+W      S   + + R G      F         +D+     E  L+
Sbjct: 1532 FHALWGCSANKGIW----KLSGFKAEIQRKGGEDVLAFLMRLARGMNQDS----YEFFLV 1591

Query: 433  ICWQIWNFRNEILHNNQQP 451
            +CWQ+W  RN   H  + P
Sbjct: 1592 LCWQVWYLRNSTKHGGRLP 1600

BLAST of Lcy08g010220 vs. ExPASy TrEMBL
Match: A0A803Q1K6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 1.6e-91
Identity = 173/466 (37.12%), Postives = 253/466 (54.29%), Query Frame = 0

Query: 5    NTNEDLVQKVKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFS 64
            N   ++   +  +L V   ++ G+YLGLPS  G NKR   + IK++VW  ++GWK   FS
Sbjct: 1028 NVTAEVRSLLAGILGVREVENHGKYLGLPSFVGRNKREFLDEIKNKVWAKMKGWKTSMFS 1087

Query: 65   AAGRETFIKAVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMC 124
             AG+E  IKA+ QAIP Y M CFKLP    + L+ + +RFWWGS +  KKIHW  W  +C
Sbjct: 1088 VAGKEVLIKAIVQAIPTYTMSCFKLPKKTISSLHRMASRFWWGSSDKEKKIHWCKWRYLC 1147

Query: 125  IHKAQGGMGFKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPS 184
              K +GG+GF+DL +FNQA+LAKQ WR +R+P+ L +RVL+  YF     L+AG G N S
Sbjct: 1148 RPKDKGGLGFRDLGMFNQALLAKQIWRCLRHPQLLCSRVLKASYFPRKGVLEAGCGANAS 1207

Query: 185  YTWRSILWGRELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKY 244
            +  RS++WG++L  KGYRWR+G G +V    DPW+PR  + K   +        V  LK 
Sbjct: 1208 FVSRSLVWGKKLILKGYRWRVGNGESVRVLEDPWLPRPVTFKVYDQPSLPANLYVTDLKM 1267

Query: 245  PSGYWREGLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKN 304
              G W EG +R +F   DA  IL IP S     D+I+W+    G ++V+S YR+      
Sbjct: 1268 ADGQWDEGSIRSIFNPTDADLILGIPCSDWDFEDKILWHYSKNGEYSVKSGYRMAASLTT 1327

Query: 305  AQNASTSKNSYKENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLC 364
             Q+ S   +  +   WK+ W+   PPKVK   WK+ ++ +    +L  RG+  + +C  C
Sbjct: 1328 EQHQSNEHSIVQ--WWKKLWRLNFPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRC 1387

Query: 365  RSKT-ETTKHLFWECKIMRGMWTKFLSYSN--SLSGVDRCGWTIPNYFEGLWKESSREDT 424
             S   E+  H  WECK  +G W     Y +   + G D            L + ++  D 
Sbjct: 1388 SSHVEESVAHALWECKASKGYWRVSGLYDDLKLILGEDNLSM--------LMRIAAEWDK 1447

Query: 425  EDRRIEKSLIICWQIWNFRNEILHNNQQPSQNKLSQQIRNAFIKFQ 468
            E  ++E  L++ W IWN RN ++H    P   ++ +   N    F+
Sbjct: 1448 E--KLEFFLLVSWNIWNVRNTVVHGGYHPKPEEMIEWCGNFLAYFR 1481

BLAST of Lcy08g010220 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 362.5 bits (929), Expect = 5.6e-96
Identity = 181/449 (40.31%), Postives = 254/449 (56.57%), Query Frame = 0

Query: 14  VKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIK 73
           ++++L V+  +   QYLGLP+    N+R  FN IKDRVW+ LQGWK K FS  G+E  IK
Sbjct: 528 IQNILSVNMVECQLQYLGLPTFMPRNRRMHFNYIKDRVWKHLQGWKAKLFSIGGKEVLIK 587

Query: 74  AVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMG 133
           AVAQAIP Y M CF+LP  L  E + + ARFWWGS +  KKIHW +W  + + K +GGMG
Sbjct: 588 AVAQAIPCYTMSCFRLPKRLIREFHHITARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMG 647

Query: 134 FKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWG 193
           F+DL +FN+A+LAKQ WRI+ +P S+L+RVL+GRYFK   F++A    NPSY WRSILWG
Sbjct: 648 FRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWG 707

Query: 194 RELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPI--PRNIEAYKYTVAQLKYPSGYWRE 253
           R+L KKG RWRIG G +V    D W+P + + K +  PR +       + + +  G W+ 
Sbjct: 708 RDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPR-LPLVSRVSSLVDHEEGGWQG 767

Query: 254 GLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTS 313
            +VRD F  ++AK IL+IPI      D +IWN +  G+++VRS Y++         A +S
Sbjct: 768 DVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSS 827

Query: 314 KNSYKENI-WKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTET 373
            +S +    W  FWK   P K+KV  W++  D + T  +L  RG+++   C  C    E 
Sbjct: 828 SSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGED 887

Query: 374 TKHLFWECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIEKSL 433
           + HLFW CK    +W       NS  G              L    S E       E+  
Sbjct: 888 SIHLFWICKFAEALWI------NSKFG---------KLSPFLILRESHESLSKADFEELC 947

Query: 434 IICWQIWNFRNEILHNNQQPSQNKLSQQI 460
           ++ W +WN RN    N+   +  K+  ++
Sbjct: 948 VVIWGLWNQRNARAFNDSTKTVFKIGMEL 960

BLAST of Lcy08g010220 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 354.0 bits (907), Expect = 2.0e-93
Identity = 178/469 (37.95%), Postives = 264/469 (56.29%), Query Frame = 0

Query: 3   SPNTNEDLVQKVKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKF 62
           S  T  D +  + ++ Q+       +YLGLPS  G   ++ FN +K RV   +  W+ KF
Sbjct: 523 SKGTRNDQITAISEIFQLKVVSRHEKYLGLPSMIGRKTKSFFNEVKLRVLNKISSWQHKF 582

Query: 63  FSAAGRETFIKAVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEK 122
           F++ G+E  IKAVAQAIP Y M  FK+PL L  ++    ARFWWG+++  K IHW  WE+
Sbjct: 583 FTSGGKEVLIKAVAQAIPTYAMSVFKIPLGLCEDIQKAMARFWWGTKQDRKGIHWARWER 642

Query: 123 MCIHKAQGGMGFKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYN 182
           +   KA+GGMGF+DLS FNQA++AKQ WRI++ P SL+ARVL+ RYFK   F+ AG G  
Sbjct: 643 ISHSKARGGMGFRDLSSFNQALVAKQGWRIMQFPSSLVARVLKARYFKHTGFMNAGLGSK 702

Query: 183 PSYTWRSILWGRELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQL 242
           PS+ WRSI+WGR++  KG RWRIG G NV    + WIPR  + KPI         TVA+L
Sbjct: 703 PSFVWRSIVWGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMGTDTTVAEL 762

Query: 243 KYPSGYWREGLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQG 302
                 WRE L+   F  EDA+AI+ IP+      D++IW+ D KG ++V+S Y++  + 
Sbjct: 763 IDEKQQWREDLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKSGYQVAMRI 822

Query: 303 KNAQNASTSKNSYKENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCL 362
           K  ++ S S  ++ +N+W+  WK   P KVK+  W+  +D++ T  +L  + +   P+C 
Sbjct: 823 KFPEDPSCS--NHDQNLWRFIWKLAIPEKVKIFLWRAAHDLLPTAENLWKKKVLQEPMCQ 882

Query: 363 LCRSKTETTKHLFWECKIMRGMWTKFLSYSNSLSGVDRCG--WTIPNYFEGLWKESSRED 422
            C    ET  H   EC   R +W ++ + +  L GV RC   W +       W    R+ 
Sbjct: 883 SCHCHVETVSHALVECNRARKIW-RYSNLAEELRGVYRCDIVWMLQ-----FW---PRQH 942

Query: 423 TEDRRIEKSLIICWQIWNFRNEILHNNQQPSQNKLSQQIRNAFIKFQRI 470
            +    E + ++ W IW  RN+ L   ++ +  ++          F++I
Sbjct: 943 AKVEGAEVAALL-WAIWKARNKWLFEGKKENPLRVVANAEAIVESFKKI 979

BLAST of Lcy08g010220 vs. NCBI nr
Match: XP_030508852.1 (uncharacterized protein LOC115723496 [Cannabis sativa])

HSP 1 Score: 353.2 bits (905), Expect = 3.4e-93
Identity = 179/474 (37.76%), Postives = 256/474 (54.01%), Query Frame = 0

Query: 3    SPNTNEDLVQKVKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKF 62
            S NT +      KD+L +       QYLGLPS SG NK+ +F  I D++W+ L  WK   
Sbjct: 737  SENTRQHEQIFFKDLLGMPIQPCHEQYLGLPSFSGKNKKQLFGGITDKIWKLLSSWKEHL 796

Query: 63   FSAAGRETFIKAVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEK 122
            FSA G+E  +KAV QAIP Y M CF+LP+TL +++ S+ ARFWWGS   GK IHW++W  
Sbjct: 797  FSAGGKEVLLKAVVQAIPTYAMSCFRLPVTLCHQIESMMARFWWGSTATGKTIHWKNWNF 856

Query: 123  MCIHKAQGGMGFKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYN 182
            +C  K QGG+GF++   FNQA+LAKQ+WRI+  P SLL+ +LR RYF  G++L AG G N
Sbjct: 857  LCKAKVQGGLGFRNFIHFNQALLAKQAWRILEFPNSLLSNLLRHRYFSNGNYLIAGLGSN 916

Query: 183  PSYTWRSILWGRELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQL 242
            PS TWRS++WG+EL  KG RWR+G G  +   +D W+P   + KP           VA L
Sbjct: 917  PSLTWRSLVWGKELLLKGLRWRVGSGERINCKTDSWLPGHTTFKPYFFKGPDPNLLVADL 976

Query: 243  KYPSGYWREGLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQG 302
                  W    +   F + D   +L+IP+SP  + D +IWN    G++ V+S Y      
Sbjct: 977  ITEHRTWDMISLETNFNQADINRVLSIPLSPYPHDDVLIWNQSFTGVYNVKSGYHFA-VS 1036

Query: 303  KNAQNASTSKNSYKENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCL 362
               Q+ ST  NS  E+ W  FWK K PPKV++  WK+++  +   + L  R +  +P C 
Sbjct: 1037 LAEQDDSTCSNSI-EHWWSNFWKLKLPPKVRIFVWKVFHTSLPVAAELYRRHIAFSPYCT 1096

Query: 363  LCRSKTETTKHLFWECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDT- 422
            +C S  ET  H  + C   + +W +  ++S     ++R               SS  DT 
Sbjct: 1097 ICNSCEETVHHALFSCPRAKAVW-ELSNFSIDFQTIER---------------SSTADTL 1156

Query: 423  -------EDRRIEKSLIICWQIWNFRNEILHNNQQPSQNKLSQQIRNAFIKFQR 469
                       +E  L++CW IW+ RN I H N   +   ++    +   +FQ+
Sbjct: 1157 LLLSTSLSSSELELFLVLCWSIWHERNAIYHGNSVRTPAAVAAYAPSYLTEFQQ 1192

BLAST of Lcy08g010220 vs. NCBI nr
Match: XP_030509135.1 (uncharacterized protein LOC115723805 [Cannabis sativa])

HSP 1 Score: 347.1 bits (889), Expect = 2.5e-91
Identity = 174/457 (38.07%), Postives = 250/457 (54.70%), Query Frame = 0

Query: 3   SPNTNEDLVQKVKDVLQVHYTDSLGQYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKF 62
           SPNT + + Q++  +L +   D++ +YLGLP   G +K+  FN I DRVW  L  W  KF
Sbjct: 172 SPNTTQAIKQEIATLLGIPVRDTMDKYLGLPQAFGRSKKDAFNYINDRVWAHLNKWNSKF 231

Query: 63  FSAAGRETFIKAVAQAIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEK 122
           FS  G+E  +K+V QAIP+Y M CFKLP +  +++ SL ARFWWG  E  KKIHW++W  
Sbjct: 232 FSKGGKEVLLKSVVQAIPSYAMACFKLPASFHSKIESLMARFWWGGNENNKKIHWKTWSS 291

Query: 123 MCIHKAQGGMGFKDLSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYN 182
           +C  K  GG+GF+ +  FNQAMLAKQ+WR+++ P SL+A +L+ RYF    FL +G G+ 
Sbjct: 292 LCFSKFHGGLGFRSMKAFNQAMLAKQAWRLLQYPHSLVAILLKARYFPQTSFLHSGKGHR 351

Query: 183 PSYTWRSILWGRELFKKGYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQL 242
           PS  W SI WG+EL + G R  IG G  +   +D WIP  G    +  N  A   TVA L
Sbjct: 352 PSLVWSSITWGKELLQSGLRKSIGDGTTINIFNDAWIPGYGKLHYLRYNSNA-DMTVASL 411

Query: 243 KYPSGYWREGLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQG 302
             P   W   L++ LF  + ++AI  IP+ P    D   W+L P G +TV S Y L    
Sbjct: 412 LTPDKQWNHALIQSLFPSDISQAITAIPLHPISIHDTYFWSLKPHGSYTVNSGYHLAHTK 471

Query: 303 KNAQNASTSKNSYKENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCL 362
            +    S+S +    + WK+ W    PPK+K   W++  DI+ T  +L NR    +P C 
Sbjct: 472 LHQNEPSSSDSKSSNDWWKKLWALPLPPKLKHFLWRVCYDILPTSRNLFNRKTLHSPHCS 531

Query: 363 LCRSKTETTKHLFWECKIMRGMW--TKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSRED 422
            C    ET +H  + C   + +W  T F ++ N+      C +   N  + L+  +    
Sbjct: 532 RCHYHDETLEHALFRCPASQKVWNLTDFNNFINA-----HCSF---NCMDLLYLAA---- 591

Query: 423 TEDRRIEKSLIIC--WQIWNFRNEILHNNQQPSQNKL 456
           TE      SL +C  W++WN RN  +H    PS +++
Sbjct: 592 TEFSTGHYSLFVCLLWKLWNCRNSWIHEGISPSPDQV 615

BLAST of Lcy08g010220 vs. NCBI nr
Match: XP_022155286.1 (uncharacterized protein LOC111022423 [Momordica charantia])

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-89
Identity = 160/311 (51.45%), Postives = 210/311 (67.52%), Query Frame = 0

Query: 28  QYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIKAVAQAIPNYPMGCF 87
           +YL L ++S   +  IF+ I DRVW+ LQGWKGK FS  G+E  IK VAQAIPNY + CF
Sbjct: 417 RYLRLVNKS---RSEIFSAIVDRVWKKLQGWKGKLFSMGGKEVLIKTVAQAIPNYTLSCF 476

Query: 88  KLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKDLSIFNQAMLAK 147
           KLP+++ NEL+ +  RFWWGS    +KIHW+SW+ +CI K  GGMGF+D+SIFNQAMLAK
Sbjct: 477 KLPVSICNELHQIYNRFWWGSSSEDRKIHWQSWKLLCIRKDLGGMGFRDISIFNQAMLAK 536

Query: 148 QSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGRELFKKGYRWRIGR 207
           QSWRI+R+P+SLLA+ LRG+YFKTG FL+A  G  PSY WRSILWGR+LFKKGYRW++G 
Sbjct: 537 QSWRILRHPDSLLAKTLRGKYFKTGSFLQAKLGARPSYAWRSILWGRDLFKKGYRWKVGN 596

Query: 208 GVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREGLVRDLFLEEDAKAIL 267
           G ++  +SDPW+PR+G+  P+  +I    ++VA L    G W E  VR+ F+  +A  IL
Sbjct: 597 GFSINLSSDPWLPRDGNFSPVFTHIHVRNFSVAWLMEGRGRWDEIKVRESFIVSEADIIL 656

Query: 268 NIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSKNSYKENIWKRFWKSK 327
             P+      DEIIW +D  G+F+VRSAY LG Q            + K ++ + F K  
Sbjct: 657 QTPLPSQDKDDEIIWGMDKTGIFSVRSAYHLGIQ--LMLKLLPQIQTLKLSVGRDFGKLM 716

Query: 328 TPPKVKVCGWK 339
            P K + C  K
Sbjct: 717 LPQKSESCAGK 722

BLAST of Lcy08g010220 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 7.0e-52
Identity = 115/384 (29.95%), Postives = 188/384 (48.96%), Query Frame = 0

Query: 78  AIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKDL 137
           A+P Y M CF LP T+  ++ S+ A FWW +++  K +HW++W+ +  +KA+GG+GFKD+
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 138 SIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGRELF 197
             FN A+L KQ WR++  PESL+A+V + RYF     L A  G  PS+ W+SI   +E+ 
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 198 KKGYRWRIGRGVNVEAASDPWI-PREGSCKPIPRNIEAYKYT-------VAQLKYPSG-Y 257
           ++G R  +G G ++      W+  +  S     + +   +Y        V+ L   SG  
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGRE 181

Query: 258 WREGLVRDLFLEEDAKAILNIPISPSQNLDEIIWNLDPKGLFTVRSAYRLGRQGKNAQNA 317
           WR+ ++  LF E + K I  +     + LD   W+    G +TV+S Y +  Q  N +++
Sbjct: 182 WRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSS 241

Query: 318 STSKNSYKEN-IWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSK 377
               +    N I+++ WKS+T PK++   WK  ++ +     L  R L     C+ C S 
Sbjct: 242 PQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSC 301

Query: 378 TETTKHLFWECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKESSREDTEDRRIE 437
            ET  HL ++C   R  W    S    L G     W    Y    W  +           
Sbjct: 302 KETVNHLLFKCTFARLTWA-ISSIPIPLGG----EWADSIYVNLYWVFNLGNGNPQWEKA 361

Query: 438 KSLI--ICWQIWNFRNEILHNNQQ 450
             L+  + W++W  RNE++   ++
Sbjct: 362 SQLVPWLLWRLWKNRNELVFRGRE 380

BLAST of Lcy08g010220 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 146.7 bits (369), Expect = 4.5e-35
Identity = 70/152 (46.05%), Postives = 92/152 (60.53%), Query Frame = 0

Query: 78  AIPNYPMGCFKLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKA-QGGMGFKD 137
           A+P Y M CF+L   L  +L S    FWW S E  +KI W +W+K+C  K   GG+GF+D
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 138 LSIFNQAMLAKQSWRIIRNPESLLARVLRGRYFKTGHFLKAGAGYNPSYTWRSILWGREL 197
           L  FNQA+LAKQS+RII  P +LL+R+LR RYF     ++   G  PSY WRSI+ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 198 FKKGYRWRIGRGVNVEAASDPWIPREGSCKPI 229
             +G    IG G++ +   D WI  E    P+
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPLPPL 153

BLAST of Lcy08g010220 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-22
Identity = 86/304 (28.29%), Postives = 128/304 (42.11%), Query Frame = 0

Query: 164 LRGRYFKTGHFLKAGAGYNPSYTWRSILWGRELFKKGYRWRIGRGVNVEAASDPWIPREG 223
           ++ RYFK    L A      SY W S+L G  L KKG R  IG G N+    D  +    
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIV---D 60

Query: 224 SCKPIPRNI-EAYK-YTVAQLKYPSG---YWREGLVRDLFLEEDAKAILNIPISPSQNLD 283
           S  P P N  E YK  T+  L    G   +W +  +     + D   I  I ++ S+  D
Sbjct: 61  SHPPRPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPD 120

Query: 284 EIIWNLDPKGLFTVRSAYRLGRQGKNAQNASTSKNSYKENIWKRFWKSKTPPKVKVCGWK 343
           +IIWN +  G +TVRS Y L     +    + +      ++  R W     PK+K   W+
Sbjct: 121 KIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWR 180

Query: 344 IYNDIIRTCSHLINRGLDVNPLCLLCRSKTETTKHLFWECKIMRGMWTKFLSYSNSLSGV 403
             +  + T   L  RG+ ++P C  C  + E+  H  + C      W   LS S+ +   
Sbjct: 181 ALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWR--LSDSSLIR-- 240

Query: 404 DRCGWTIPNYFEGLWKE--SSREDTEDRRIEKSLII--CWQIWNFRNEILHN--NQQPSQ 457
                 + N FE       +  +DT      K L +   W+IW  RN ++ N   + PS+
Sbjct: 241 ---NQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSK 294

BLAST of Lcy08g010220 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 94.7 bits (234), Expect = 2.0e-19
Identity = 88/399 (22.06%), Postives = 153/399 (38.35%), Query Frame = 0

Query: 28  QYLGLPSQSGTNKRAIFNNIKDRVWRALQGWKGKFFSAAGRETFIKAVAQAIPNYPMGCF 87
           +YLGLP  +     + +  + +++   +  W  +  S AGR   I +V  ++ N+ M  F
Sbjct: 26  RYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAF 85

Query: 88  KLPLTLTNELYSLCARFWWGSEEAGKKIHWRSWEKMCIHKAQGGMGFKDLSIFNQAMLAK 147
           +LP     E+ S+C+ F W   E   K    +W  +C  K +GG+G + L   N+     
Sbjct: 86  RLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEANKGSF-- 145

Query: 148 QSWRIIRNP---ESLLARVLRGRYFKTGHFLKAGA--GYNPSY---TWRSILWGRELFKK 207
             W I  N      +  ++L+ R   +G F+K     G N S+    W  I  GR +   
Sbjct: 146 --WSISGNTTLGSWMWKKILKHRALASG-FVKHDIHNGSNTSFWFDNWSKI--GRLIDVT 205

Query: 208 GYRWRIGRGVNVEAASDPWIPREGSCKPIPRNIEAYKYTVAQLKYPSGYWREGLVRDLFL 267
           G+R  I  G+ + A+    +      +     +   +  +A++++      +GL      
Sbjct: 206 GHRGCIDMGITLHASVAEAVVNHRPRRHRHDTLLRIEDVIAEVRH------QGL------ 265

Query: 268 EEDAKAILNIPISPSQNLDEIIWNLDP---KGLFTVRSAYRLGRQGKNAQNASTSKNSYK 327
                         +   D + W  +    K  F  +  +           A+T +   K
Sbjct: 266 --------------TSGEDTVRWKGNGDIFKPCFNTKETW-----------AATREPKLK 325

Query: 328 ENIWKRFWKSKTPPKVKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTETTKHLFW 387
            N +K  W S   PK  V  W    + + T   +++     +  C+LC    ET  HLF+
Sbjct: 326 VNWYKGVWFSHATPKYSVLAWIAIKNRLTTGDRMLSWNAGADSSCVLCHHLVETRDHLFF 367

Query: 388 ECKIMRGMWTKFLSYSNSLSGVDRCGWTIPNYFEGLWKE 416
            C            YS  +  + R  +T       LWKE
Sbjct: 386 TC-----------PYSAEVPFLTR--YTFQLTLHSLWKE 367

BLAST of Lcy08g010220 vs. TAIR 10
Match: AT4G04650.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 50.4 bits (119), Expect = 4.4e-06
Identity = 45/194 (23.20%), Postives = 81/194 (41.75%), Query Frame = 0

Query: 275 QNLDEIIWNLD---PKGLFTVRSAYRLGRQGKNAQNASTSKNSYKENIWKRFWKSKTPPK 334
           Q+ D  +W  D   P   F+    +       +A +  +    + + +W   +K+  P  
Sbjct: 93  QHDDSFLWKTDLHAPSNRFSAPRTW-------SALHPQSHTVPWHKAVW---FKNHVPKH 152

Query: 335 VKVCGWKIYNDIIRTCSHLINRGLDVNPLCLLCRSKTETTKHLFWECKIMRGMWTKFLSY 394
             +C W +  + + T   L N GL +   CLLC +  ++  HLF+EC+    +W +F + 
Sbjct: 153 AFIC-WVVAWNRLHTRDRLQNWGLSIPAECLLCNAHDDSRAHLFFECQFSGVVW-RFFTA 212

Query: 395 SNSLSG----VDRCGWTIPNYFEGLWKESSREDTEDRRIEKSLIIC-WQIWNFRNEILHN 454
           S +L+     +D   W +           SRE      I  +   C + IW  RN+ LH+
Sbjct: 213 STNLNPPAQLMDCLNWLL---------SPSREKNICLIIRLAFHSCVYAIWRERNQRLHS 265

Query: 455 NQQPSQNKLSQQIR 461
               S   + + I+
Sbjct: 273 GVSRSTESILKDIQ 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C2F63.7e-3426.21Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P932956.4e-3446.05Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DAR42.7e-9640.31uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A803QQT26.3e-9337.17Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QEG97.0e-9238.46Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NHG39.1e-9238.27Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803Q1K61.6e-9137.12Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022150918.15.6e-9640.31uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_024037590.12.0e-9337.95uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_030508852.13.4e-9337.76uncharacterized protein LOC115723496 [Cannabis sativa][more]
XP_030509135.12.5e-9138.07uncharacterized protein LOC115723805 [Cannabis sativa][more]
XP_022155286.11.3e-8951.45uncharacterized protein LOC111022423 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G29090.17.0e-5229.95Ribonuclease H-like superfamily protein [more]
ATMG00310.14.5e-3546.05RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G09510.11.2e-2228.29Ribonuclease H-like superfamily protein [more]
AT3G24255.12.0e-1922.06RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G04650.14.4e-0623.20RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 290..385
e-value: 2.7E-18
score: 66.5
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 2..349
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 2..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g010220.1Lcy08g010220.1mRNA