Lcy08g009140 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g009140
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr08: 20870631 .. 20873680 (-)
RNA-Seq ExpressionLcy08g009140
SyntenyLcy08g009140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGCCACGTTTTGGTGGGGTACCACAGATGCACGGAAGAAATTACACTGGAAGAAATGGGAGTTTATGTGTCTTCCGAAGGAGATGGGAGGTTTGAACTTTAGAGACTTGATCTCCTTTAATCGCTCATTATTGGCAAAACAGGTTTGGCGTTTTCTCAAAAATCCACATTTATTAGCTTCTAGAGTTATCTTAGGGAGATATGCATACTAAGCATCGATATTAACGACCCCTCTTAAATCTAATAGTTCCATTTTCTGGAGAGGTTTTGTTTGGGCCCGTGAGTTGATCTTGAGGGGTATTCGAAAACGGGTTGGAAATGGTAATTCCATCAGATTTTTTGAAGACCCATGGATCCCAAAGGAAGTCACTTTTAAACCGATGCGGAACCATAATCAGGTGATGCAAGGCGTGCTTACGGTTGCTGATTTTCTTACTACATCTTATAGGTGGGACATGGAGAAGATCCGAAATGTGGTTACAGAAGAGATGCACCGGTGATCCAATCTATCCTGATAGGAGGGCCAAATGAGATGGATGCATGGATATGGCACTACTCACGGAATGGTGAGTATTCTACCAAGAGTGGATACAAGTTAGAGATGAATCGTAATGTGCATCAAGCCTCTACAAGTGTCAATGCTCATAATGTTTGGTGGAGGAAAGTGTGGAAAATGAAGATACCGTCAAAAATAAAATTATTTATATGGAAAGCCTATCATGGGTGCTTGCCAACGAATTATTGTCTCTGGAAGTGTGGTATGGATGTGTTAAGTCTCTACAGTGTTTGTGGGAGTGAGATGGAAAGTATAGATCATGCTCTTTGTGGTTGCAAGCGTGCTAAAGAAATTTGTGATATGATGTTTCCACGTGTTGATGTTGATATATTTGTGGCTGACAATTTTGCAAACAGAATCCTAAGACTGGCGGACCATCTTAGCATGGAGGTTTTTGAAAAAACATGTATTACTTTCTGGGCAATATGGAATGATAGGAATAATTTCATTTTTCAACAGCCTCTTATGGAATTGTCTGCTCGGTGTGATTGGATTCATAAGTACTGAGAGGATACTCGACAACGATCAGCACTGATTGCAACTCATCTAGACGAGTCTCGGGTCTACCACGTTGCGATGCATGCTTGAACAAGGGTGTTTACAGATGCAGCTGTTCGGCAGTATAAAGTAGGTGTTGGGTATGGGATGGTCATTGTTAACGAGGACGATACCTTGCAGAGCGCCTTGCATATGTTTGAGAACAGGTCTTTGTCGCCATTGGCTGTAGAAGTGCAAGCCATTCTGCAAGCTGTAACGTTATTGATGCGCATGCATATTGCTGAGGTTGTGTTGCACTCAGATTCTTTAAGTGCCCTTAGGATGATTAATGGGGAGCAGGAGCCAAATTCAGAGGTACATTTTTGGATCCTGCAAATTAAGGAATTGAGTAAAACGTTTACTTCTCTGATATATGTCCATGTTGGAAGATCGAGTAATGGAAGGGCGGATGCTTTAGCAAAATATGCTCTCCAAAGCACACAATCTATGTTGTGGCTATCTAATTTCCCTATTTGGCTAGTGTCAATGGGACCAAATGTCCATTGTAAATGTATTAATGTTTGTTCTTGCAATTCCTAATAAAGATCTCGTTTCTTATAAAAAAAAAAATTAAAAAAAAAAAAAACAATGGAAGAGAAAGTTTCTTGCCAGAGCTCCTTCTAAATATAGCTATAGATAGATCTGTATTTGCCACTATTAGATTTTTTAATTTTTTTTTTTTTTTAAGAAACTGTTAGAACTTTTTCTTGGACGGATCAAAGCCAGCCATCAATCTATCCATTTTCCTACCTACGTTATTGATCTAGTAAAATTTAATCATAAAATAATATTTCCAGTCAAGGTTTGACCTTACGATCACTTGATATGATATTTTTTTTTTTTTTTTTGAAAAACAATTGATATGATATTATGATATCTAGTTTCTCATTTTTTTTCAAAAAAAGATCTGGTTTATCATTTTAAATAAGAGAATGCGATATGATGGTTGAAATAAGTGTTAGAATAATCCATAATCCAAAAAATATGAAGCAAAATAACATTATAAACATGCCAAAGTAAAATTTTTACTCTTATAAACCTATTGTATTATTATCAATCATGAAATCAAATCAAATCAAGAAATTAGTTTATACTTCAATTCAAGTCGACACATCTCTAATTGATCAATAAAATATTTTATTGTGTGGTACATCATAAAATTGAATCCAAAATTTTATAGAATAAAGTAAATATTTTCAAAGTTAGAGAACAACTGTAAATAACATAAATCTGGTAACTCAATCATAAATATATTTGAAATTTGTTGAATAAAATAATAAAAATGATTGAATATACATACATGCATATTTATTGAAGAGCATGGGAATGGTTTAAATAAATAAAACATAATAGGTATACAACACACATACACAATTGAATATCGAAAGTCATATTGTCGAGGATTCCACGTTGAAAAGATAAGTGGAGACCTCATAATTTATAAGATGTATGGGTTACTCCACTCATTGTCAATTGATTTTGAGATTGAACTTCATGTTATATAATATGGTATCAGAGTCCATAAAACCCAAACGGATATTCGGTCCAAGAAATAAAAATTGGGATCCGAGAGGACATTGAGATAGAACCCATGTTAATCTAATACATATGATTTTGAAAATATATATTTTAATTTGAAGAGCATATGAATGATTGAATAAGATGGGAATATATTCATGGTAACTACCCAAATTTTTCCTTCCAAAAAAAAATATATGGGAATATATTCTTTGGAAAAAAACAAAGATGGGAATATATAATTTGTAGGAAACGTGAATGAAAAGTAGTTGTTGGGGGCAAAAGCCATTAATTTTAATTCCTACCAAAGGTGGTTGGTGGAGGCAAAAACCATTAATTTAATTAAAGAACAACAAGTTGAAACCTTAAAATAGAAAGAAAATGGAAATATCTTAACTTATAACTATATATCAATAGGTCAGAAGTATTGTTTAA

mRNA sequence

ATGATGGCCACGTTTTGGTGGGGTACCACAGATGCACGGAAGAAATTACACTGGAAGAAATGGGAGTTTATGTGTCTTCCGAAGGAGATGGGAGGTTTGAACTTTAGAGACTTGATCTCCTTTAATCGCTCATTATTGGCAAAACAGGTGGGACATGGAGAAGATCCGAAATGTGGTTACAGAAGAGATGCACCGGTGATCCAATCTATCCTGATAGGAGGGCCAAATGAGATGGATGCATGGATATGGCACTACTCACGGAATGGTGAGTATTCTACCAAGAGTGGATACAAGTTAGAGATGAATCGTAATGTGCATCAAGCCTCTACAAGTGTCAATGCTCATAATGTTTGGTGGAGGAAAGTGTGGAAAATGAAGATACCGTCAAAAATAAAATTATTTATATGGAAAGCCTATCATGGGTGCTTGCCAACGAATTATTGTCTCTGGAAGTGTGGTATGGATGTGTTAAGTCTCTACAGTGTTTGTGGGAGTGAGATGGAAAGTATAGATCATGCTCTTTGTGGTTGCAAGCGTGCTAAAGAAATTTGTGATATGATGTTTCCACGTGTTGATGTTGATATATTTGTGGCTGACAATTTTGCAAACAGAATCCTAAGACTGGCGGACCATCTTAGCATGGAGGTTTTTGAAAAAACATGTATTACTTTCTGGGCAATATGGAATGATAGGAATAATTTCATTTTTCAACAGCCTCTTATGGAATTGTCTGCTCGGGTGTTTACAGATGCAGCTGTTCGGCAGTATAAAGTAGGTGTTGGGTATGGGATGGTCATTGTTAACGAGGACGATACCTTGCAGAGCGCCTTGCATATGTTTGAGAACAGGTCTTTGTCGCCATTGGCTGTAGAAGTGCAAGCCATTCTGCAAGCTGTAACGTTATTGATGCGCATGCATATTGCTGAGGTTGTGTTGCACTCAGATTCTTTAAGTGCCCTTAGGATGATTAATGGGGAGCAGGAGCCAAATTCAGAGGTCAGAAGTATTGTTTAA

Coding sequence (CDS)

ATGATGGCCACGTTTTGGTGGGGTACCACAGATGCACGGAAGAAATTACACTGGAAGAAATGGGAGTTTATGTGTCTTCCGAAGGAGATGGGAGGTTTGAACTTTAGAGACTTGATCTCCTTTAATCGCTCATTATTGGCAAAACAGGTGGGACATGGAGAAGATCCGAAATGTGGTTACAGAAGAGATGCACCGGTGATCCAATCTATCCTGATAGGAGGGCCAAATGAGATGGATGCATGGATATGGCACTACTCACGGAATGGTGAGTATTCTACCAAGAGTGGATACAAGTTAGAGATGAATCGTAATGTGCATCAAGCCTCTACAAGTGTCAATGCTCATAATGTTTGGTGGAGGAAAGTGTGGAAAATGAAGATACCGTCAAAAATAAAATTATTTATATGGAAAGCCTATCATGGGTGCTTGCCAACGAATTATTGTCTCTGGAAGTGTGGTATGGATGTGTTAAGTCTCTACAGTGTTTGTGGGAGTGAGATGGAAAGTATAGATCATGCTCTTTGTGGTTGCAAGCGTGCTAAAGAAATTTGTGATATGATGTTTCCACGTGTTGATGTTGATATATTTGTGGCTGACAATTTTGCAAACAGAATCCTAAGACTGGCGGACCATCTTAGCATGGAGGTTTTTGAAAAAACATGTATTACTTTCTGGGCAATATGGAATGATAGGAATAATTTCATTTTTCAACAGCCTCTTATGGAATTGTCTGCTCGGGTGTTTACAGATGCAGCTGTTCGGCAGTATAAAGTAGGTGTTGGGTATGGGATGGTCATTGTTAACGAGGACGATACCTTGCAGAGCGCCTTGCATATGTTTGAGAACAGGTCTTTGTCGCCATTGGCTGTAGAAGTGCAAGCCATTCTGCAAGCTGTAACGTTATTGATGCGCATGCATATTGCTGAGGTTGTGTTGCACTCAGATTCTTTAAGTGCCCTTAGGATGATTAATGGGGAGCAGGAGCCAAATTCAGAGGTCAGAAGTATTGTTTAA

Protein sequence

MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQVGHGEDPKCGYRRDAPVIQSILIGGPNEMDAWIWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCGSEMESIDHALCGCKRAKEICDMMFPRVDVDIFVADNFANRILRLADHLSMEVFEKTCITFWAIWNDRNNFIFQQPLMELSARVFTDAAVRQYKVGVGYGMVIVNEDDTLQSALHMFENRSLSPLAVEVQAILQAVTLLMRMHIAEVVLHSDSLSALRMINGEQEPNSEVRSIV
Homology
BLAST of Lcy08g009140 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 24/49 (48.98%), Postives = 33/49 (67.35%), Query Frame = 0

Query: 2  MATFWWGTTDARKKLHWKKWEFMCLPKE-MGGLNFRDLISFNRSLLAKQ 50
          M  FWW + + ++K+ W  W+ +C  KE  GGL FRDL  FN++LLAKQ
Sbjct: 25 MTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQ 73

BLAST of Lcy08g009140 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.5e-05
Identity = 22/45 (48.89%), Postives = 29/45 (64.44%), Query Frame = 0

Query: 4   TFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAK 49
           TF WG+T  +KK H  KW  +C PK+ GGL  R   S NR+L++K
Sbjct: 72  TFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISK 116

BLAST of Lcy08g009140 vs. ExPASy TrEMBL
Match: A0A803PYI0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 4.2e-38
Identity = 104/351 (29.63%), Postives = 175/351 (49.86%), Query Frame = 0

Query: 1    MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQ----VGHGED- 60
            M + FWWG++D  KK+HW KW ++C PK+ GGL FRDL  FN++LLAKQ    + H +  
Sbjct: 1247 MASRFWWGSSDKEKKIHWCKWRYLCRPKDKGGLGFRDLGMFNQALLAKQIWRCIRHPQQL 1306

Query: 61   ----------PKCGYRRD--APVIQSILIGGPNEMDAWIWHYSRNGEYSTKSGYKLEMNR 120
                      P+ G      A +I +I     +  D  +WHYS+NGEY+ KS Y++  + 
Sbjct: 1307 CSRVLKASYFPRKGVLEAGYAELILAIPCSDWDFEDKILWHYSKNGEYTVKSVYRMASSL 1366

Query: 121  NVHQASTSVNAHNVWWRKVWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVC 180
            +  Q  ++ ++   WW+K+W++KIP K+K F+WK  H  LPTN  L K G++   + S C
Sbjct: 1367 STEQHQSNDHSLVQWWKKLWRLKIPPKVKHFVWKVAHNWLPTNVNLAKRGINSGVICSRC 1426

Query: 181  GSEM-ESIDHALCGCKRAKEICDMMFPRVDVDIFVADNFANRILRLADHLSMEVFEKTCI 240
             S + ESI HAL GCK  K    +     +    +  +  + ++R+A     E      +
Sbjct: 1427 SSHVDESIAHALWGCKANKGYWVVSGVYDEFKKMLGTDNLSLLMRMAAEWEKEKLIFFLL 1486

Query: 241  TFWAIWNDRNNFIF------QQPLMELSARVFTDAAVRQYKVGVGYGMVIVNEDDTLQSA 300
              W +WN RN+ +        + ++E   R          ++ +  G+        L   
Sbjct: 1487 VSWNVWNVRNSVVHGGYHPKPEDMIEWCGR----------RIALWPGVCNEIFYCALLCL 1546

Query: 301  LHMFENRSLSPLAVEVQAILQAVTLLMRMHIAEVVLHSDSLSALRMINGEQ 328
            L +   + L PL +E+ AI + +   ++  +    + +D L A+++I  ++
Sbjct: 1547 LLLMLKQELPPLQLELMAIKKGLQAGLQRRLRRFNVETDCLQAVQLIQNKE 1587

BLAST of Lcy08g009140 vs. ExPASy TrEMBL
Match: A0A803QHJ7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 7.9e-37
Identity = 106/344 (30.81%), Postives = 167/344 (48.55%), Query Frame = 0

Query: 1   MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQVGHG------- 60
           M A FWWG+++  KK+HW KWE +C PK+ GG+ F DL  FN++LLAKQ+          
Sbjct: 350 MTARFWWGSSEKDKKIHWCKWEHLCKPKDKGGMGFCDLGMFNQALLAKQIWRCIRYPTAL 409

Query: 61  -----EDPKCGYRRDAPVIQSILIGGPNEMDAWIWHYSRNGEYSTKSGYKLEMNRNVHQA 120
                +      + DA +I S+   G +  D  +WHYS+NGEYS KSGY++       + 
Sbjct: 410 CNRVLKASAVFNKDDAEMILSLPNSGWDIDDKILWHYSKNGEYSVKSGYRMACELKAERQ 469

Query: 121 STSVNAHNVWWRKVWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCG-SEM 180
            +  +    WWRK+W++KIP KIK+F+WK  HG LPT+  L K  +      S C     
Sbjct: 470 QSDDHLAVQWWRKLWRLKIPQKIKVFVWKLAHGWLPTSTVLAKRHVSTTGGCSRCSPHNS 529

Query: 181 ESIDHALCGCKRAKEICDMMFPRVDVDIFVADNFANRILRLAD---HLSMEVFEKTCITF 240
           E I HA   CK++K + +        +  +   F  +   + +    L  +     C   
Sbjct: 530 EIIFHAFWECKKSKSLWNTR------NACIHGGFVPQPAEMVEWCYKLLGDFQGGGCRPQ 589

Query: 241 WAIWNDRNNFIFQQPLMELSARVFTDAAVRQYKVGVGYGMVIVNEDDTLQSALHMFENRS 300
            A+   R   +++ P      ++  D  V++     G G VI  E   +  A      R 
Sbjct: 590 EAM--RREEGVWKVP-RHGEVKINVDTGVKKGAGYSGLGCVIRGEQGDVLFASSTAIPRE 649

Query: 301 LSPLAVEVQAILQAVTLLMRMHIAEVVLHSDSLSALRMINGEQE 329
            SPL +E+ AIL+ +   ++ HI    + SD L A+++I  +++
Sbjct: 650 YSPLQLELMAILKGIQAGLQRHIMHFSIESDCLEAIQLIQKKED 684

BLAST of Lcy08g009140 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.1e-36
Identity = 122/460 (26.52%), Postives = 176/460 (38.26%), Query Frame = 0

Query: 14   KKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAK------------------------- 73
            +KLHW KW  MC PKE GGLNFRDL  FN++L+AK                         
Sbjct: 927  RKLHWMKWGRMCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDT 986

Query: 74   -------------------------------QVGHGEDPK-------------------- 133
                                           +VG+G   K                    
Sbjct: 987  SLLQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNN 1046

Query: 134  --------------------------CGYRRDAPVIQSILIGGPNEMDAWIWHYSRNGEY 193
                                      C   RD  +I S+ I   N  D+W+WHY + G Y
Sbjct: 1047 GALDTTVASFITADGNWDVTSISHSFCNEDRD--LILSMPISSYNLQDSWLWHYDKRGNY 1106

Query: 194  STKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKLFIWKAYHGCLPTNYCLWK 253
            S +SGYKL M+   +  S S N     W  +WK+ +P+KIK+FIW++ H  +PT   L  
Sbjct: 1107 SVRSGYKLYMHLKCNATSASTNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLL 1166

Query: 254  CGMDVLSLYSVCGSEMESIDHALCGCKRAKEICDMMFPRVDVDIFVADN--FANRILRLA 313
             G+  L   ++CG   ESI HA   CKRA++I   +FP +   +   DN  F      L 
Sbjct: 1167 RGIGELPACTICGDRRESIIHAFFHCKRARQIWRTLFPFLTC-LSAEDNISFLELWSSLT 1226

Query: 314  DHLSMEVFEKTCITFWAIWNDRNNFI---------------------------------- 327
            + L  +      IT W IWNDRN+ I                                  
Sbjct: 1227 EQLEPKDLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRT 1286

BLAST of Lcy08g009140 vs. ExPASy TrEMBL
Match: A0A803Q185 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 1.9e-35
Identity = 127/473 (26.85%), Postives = 195/473 (41.23%), Query Frame = 0

Query: 1   MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQ----------- 60
           M A FWWG+++  KK+HW KWE +C PK+ GG+ FRDL  FN++LLAKQ           
Sbjct: 46  MAARFWWGSSEKDKKIHWCKWEHLCKPKDKGGMGFRDLGMFNQALLAKQIWRCIRYPNAL 105

Query: 61  ---------------------------------------------VGHG------EDP-- 120
                                                        VG G      EDP  
Sbjct: 106 CNRVLKASYFPTNSVLEAKCGTHASFVWRSLMWGKKLILKGYRWRVGDGSNIRVLEDPWL 165

Query: 121 -----------------------KCG-------------YRRDAPVIQSILIGGPNEMDA 180
                                  K G              + DA +I ++   G +  D 
Sbjct: 166 PRPVTFKIYDKPPLPEHLWVVDLKLGDGTWDKPFISAVFNKDDAEMILALPNSGWDLDDK 225

Query: 181 WIWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKLFIWKAYH 240
            +WHY +NGEYS KSGY++       +  +  +    WWRK+W++KIP KIK+F+WK  H
Sbjct: 226 ILWHYCKNGEYSVKSGYRMACELKAERQQSDDHLAVQWWRKLWRLKIPPKIKVFVWKLAH 285

Query: 241 GCLPTNYCLWKCGMDVLSLYSVCG-SEMESIDHALCGCKRAK---EICDMMFPRVDVDIF 300
           G LPT+  L K  +      S C     E+I HAL  CK++K   ++CD    ++++   
Sbjct: 286 GWLPTSTVLAKRHVSTTDGCSRCSLHNSETIFHALWECKKSKVCWKLCDF---QLEIKRH 345

Query: 301 VADNFANRILRLADHLSMEVFEKTCITFWAIWNDRN-----NFIFQ-------------- 329
             ++    ++RL+  +S + FE   +  W++WN RN      F+ Q              
Sbjct: 346 GQEDELAFLMRLSTVMSKDQFEMFLVITWSLWNTRNACTHDGFVPQPAEMVEWCYKLLED 405

BLAST of Lcy08g009140 vs. ExPASy TrEMBL
Match: A0A803NI87 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 3.3e-35
Identity = 107/428 (25.00%), Postives = 176/428 (41.12%), Query Frame = 0

Query: 1   MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAK----QVGHGE-- 60
           MMA FWWG+   +KK HW+KW+ +C  K  GG      I + R LL K    ++G G   
Sbjct: 554 MMANFWWGSDKDKKKTHWRKWKLLCKTKMDGG------ICWGRDLLVKGLRLKIGDGSSV 613

Query: 61  ---------------------DPK-------------------CGYRR-DAPVIQSILIG 120
                                DP+                   C +   D   I SI + 
Sbjct: 614 TCSSDPWIPRHSEFTPFYYSGDPQNLVSFYITEDKEWNLPLLTCDFSAVDIDFILSIPLS 673

Query: 121 GPNEMDAWIWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKL 180
             +  D WIWH++ + EY+ ++GY L    +  +  TS  + + WW+  W +K+PSK+K+
Sbjct: 674 SSHVRDQWIWHFTNSSEYTVQTGYHLATELDESELPTSSTSQHSWWKYFWNLKLPSKVKI 733

Query: 181 FIWKAYHGCLPTNYCLWKCGMDVL--SLYSVCGSEMESIDHALCGCKRAKEICDMMFPRV 240
           F+WKA+H  +PT   L  C M ++  +  S+C +  ESI HAL  CK AK++       +
Sbjct: 734 FVWKAFHRAIPTAASL--CNMKIIDSAACSLCSNAWESIKHALLLCKHAKKVWHSAGYSL 793

Query: 241 DVDIFVADNFANRILRLADHLSMEVFEKTCITFWAIWNDRNNFIFQQPLMELS------- 300
           D D+     F++ ++ L+   +    E+   T W +W DRNN+I  +P++  S       
Sbjct: 794 DYDMLARMKFSDCLVLLSTLKTTSEMEQMFCTMWFLWTDRNNYIHGKPVLSPSQIWAKSA 853

Query: 301 -----------------------------------ARVFTDAAVRQYKVGVGYGMVIVNE 338
                                               ++  DAA    +  +G G+++ N 
Sbjct: 854 AYYCNFQHHSTIQNSPMISTGAATQSKWIRPPLNELKMNVDAACDVSRNKIGVGIIVRNS 913

BLAST of Lcy08g009140 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-35
Identity = 122/460 (26.52%), Postives = 176/460 (38.26%), Query Frame = 0

Query: 14   KKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAK------------------------- 73
            +KLHW KW  MC PKE GGLNFRDL  FN++L+AK                         
Sbjct: 927  RKLHWMKWGRMCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDT 986

Query: 74   -------------------------------QVGHGEDPK-------------------- 133
                                           +VG+G   K                    
Sbjct: 987  SLLQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNN 1046

Query: 134  --------------------------CGYRRDAPVIQSILIGGPNEMDAWIWHYSRNGEY 193
                                      C   RD  +I S+ I   N  D+W+WHY + G Y
Sbjct: 1047 GALDTTVASFITADGNWDVTSISHSFCNEDRD--LILSMPISSYNLQDSWLWHYDKRGNY 1106

Query: 194  STKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKLFIWKAYHGCLPTNYCLWK 253
            S +SGYKL M+   +  S S N     W  +WK+ +P+KIK+FIW++ H  +PT   L  
Sbjct: 1107 SVRSGYKLYMHLKCNATSASTNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLL 1166

Query: 254  CGMDVLSLYSVCGSEMESIDHALCGCKRAKEICDMMFPRVDVDIFVADN--FANRILRLA 313
             G+  L   ++CG   ESI HA   CKRA++I   +FP +   +   DN  F      L 
Sbjct: 1167 RGIGELPACTICGDRRESIIHAFFHCKRARQIWRTLFPFLTC-LSAEDNISFLELWSSLT 1226

Query: 314  DHLSMEVFEKTCITFWAIWNDRNNFI---------------------------------- 327
            + L  +      IT W IWNDRN+ I                                  
Sbjct: 1227 EQLEPKDLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRT 1286

BLAST of Lcy08g009140 vs. NCBI nr
Match: XP_030505962.1 (uncharacterized protein LOC115720894 [Cannabis sativa])

HSP 1 Score: 159.1 bits (401), Expect = 6.8e-35
Identity = 107/428 (25.00%), Postives = 176/428 (41.12%), Query Frame = 0

Query: 1    MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAK----QVGHGE-- 60
            MMA FWWG+   +KK HW+KW+ +C  K  GG      I + R LL K    ++G G   
Sbjct: 693  MMANFWWGSDKDKKKTHWRKWKLLCKTKMDGG------ICWGRDLLVKGLRLKIGDGSSV 752

Query: 61   ---------------------DPK-------------------CGYRR-DAPVIQSILIG 120
                                 DP+                   C +   D   I SI + 
Sbjct: 753  TCSSDPWIPRHSEFTPFYYSGDPQNLVSFYITEDKEWNLPLLTCDFSAVDIDFILSIPLS 812

Query: 121  GPNEMDAWIWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKL 180
              +  D WIWH++ + EY+ ++GY L    +  +  TS  + + WW+  W +K+PSK+K+
Sbjct: 813  SSHVRDQWIWHFTNSSEYTVQTGYHLATELDESELPTSSTSQHSWWKYFWNLKLPSKVKI 872

Query: 181  FIWKAYHGCLPTNYCLWKCGMDVL--SLYSVCGSEMESIDHALCGCKRAKEICDMMFPRV 240
            F+WKA+H  +PT   L  C M ++  +  S+C +  ESI HAL  CK AK++       +
Sbjct: 873  FVWKAFHRAIPTAASL--CNMKIIDSAACSLCSNAWESIKHALLLCKHAKKVWHSAGYSL 932

Query: 241  DVDIFVADNFANRILRLADHLSMEVFEKTCITFWAIWNDRNNFIFQQPLMELS------- 300
            D D+     F++ ++ L+   +    E+   T W +W DRNN+I  +P++  S       
Sbjct: 933  DYDMLARMKFSDCLVLLSTLKTTSEMEQMFCTMWFLWTDRNNYIHGKPVLSPSQIWAKSA 992

Query: 301  -----------------------------------ARVFTDAAVRQYKVGVGYGMVIVNE 338
                                                ++  DAA    +  +G G+++ N 
Sbjct: 993  AYYCNFQHHSTIQNSPMISTGAATQSKWIRPPLNELKMNVDAACDVSRNKIGVGIIVRNS 1052

BLAST of Lcy08g009140 vs. NCBI nr
Match: XP_030509188.1 (uncharacterized protein LOC115723863 [Cannabis sativa])

HSP 1 Score: 157.1 bits (396), Expect = 2.6e-34
Identity = 89/291 (30.58%), Postives = 129/291 (44.33%), Query Frame = 0

Query: 1    MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQV---------- 60
            MMA FWWG++ + K +HWK W F+C  K  GGL FR+ I FN++LLAKQ           
Sbjct: 834  MMANFWWGSSASGKSIHWKNWNFLCKAKVQGGLGFRNFIHFNQALLAKQAWRLIEFPNSL 893

Query: 61   ------------------GHGEDPKCGYRR------------------------------ 120
                              G G +P   +R                               
Sbjct: 894  LSKLLRHRYFSNGRFLSSGLGSNPSLTWRSLGADPNLLVADLITEQRQWDLISLRANFSQ 953

Query: 121  -DAPVIQSILIGGPNEMDAWIWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNVWWRK 180
             D   I SI +      DA IW +S  G Y+ KSGY+L ++      + S ++   WW  
Sbjct: 954  PDVDRILSIPLSLFPHDDAMIWSHSFTGIYNVKSGYQLAVSYAEQDDTASSHSMENWWST 1013

Query: 181  VWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCGSEMESIDHALCGCKRAK 233
             WKMK+P K+++F+WK +H  LP    L++  +      S+C S  ESI+HAL  C RAK
Sbjct: 1014 FWKMKLPPKVRIFVWKVFHSTLPVAAELFRRHIATSPHCSICNSAEESINHALFDCPRAK 1073

BLAST of Lcy08g009140 vs. NCBI nr
Match: XP_030496655.1 (uncharacterized protein LOC115712508 [Cannabis sativa])

HSP 1 Score: 153.7 bits (387), Expect = 2.9e-33
Identity = 98/353 (27.76%), Postives = 159/353 (45.04%), Query Frame = 0

Query: 1   MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQVGH-GEDPK-- 60
           +M+ FWWG+T  +KK+HWK+W+F+C  K  GGL FR+ I FN++LLAKQ     ++P+  
Sbjct: 17  LMSNFWWGSTSDKKKIHWKQWKFLCKSKLEGGLGFRNFIHFNQALLAKQAWRLFQNPESL 76

Query: 61  --------------------CGY------------------------RRDAPVIQSILIG 120
                               CG                           D   I S+ + 
Sbjct: 77  LFRVLKGRYFSRSDFLSAATCGVSSLTWQGICWGRELLKKGLRLQVGNADVERILSLPLS 136

Query: 121 GPNEMDAWIWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNV-WWRKVWKMKIPSKIK 180
                D W+WH +  G Y  KSGY +       +   SV++ NV WW+  W++K+P K+K
Sbjct: 137 HHAHSDYWVWH-ATGGHYEVKSGYHVAC-MLADENPVSVSSPNVSWWKSFWQLKLPPKVK 196

Query: 181 LFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCGSEMESIDHALCGCKRAKEICDMMFPRVD 240
           LF WKA H  LP    L+K      +  S+C +  ES+ HA+  CK A+ +  +     +
Sbjct: 197 LFAWKAIHNALPVASELYKRKSLTSASCSLCLNAWESVGHAMFACKHARHVWKIAGFSFN 256

Query: 241 VDIFVADNFANRILRLADHLSMEVFEKTCITFWAIWNDRNNFIFQQ---------PLMEL 297
               V+    + + ++++    EV    C T W+IW+DRNN +            P    
Sbjct: 257 NKAAVSMKIEDFLFQISNATPNEVGNDFC-TMWSIWSDRNNVLHADTPTTHRAWTPPPPN 316

BLAST of Lcy08g009140 vs. NCBI nr
Match: XP_030479133.1 (uncharacterized protein LOC115696372 [Cannabis sativa])

HSP 1 Score: 147.1 bits (370), Expect = 2.7e-31
Identity = 92/336 (27.38%), Postives = 127/336 (37.80%), Query Frame = 0

Query: 1    MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQ----------- 60
            MMA FWWG++   KK+HWKKW F+C  K  GG+ FR  + FN++LLAKQ           
Sbjct: 785  MMAKFWWGSSSDNKKIHWKKWRFLCKSKGDGGMGFRSFVHFNQALLAKQAWRIFQDPTSL 844

Query: 61   ---------------------------------------------VGHGEDPKC------ 120
                                                         VG G + +C      
Sbjct: 845  LSRVLKGHYFSQNDFMTARGGGLSSLTWQGIVWGRELLVKGLRLKVGTGNNIRCAVDSWI 904

Query: 121  ----GYR---------------------------------RDAPVIQSILIGGPNEMDAW 180
                G++                                  D   I  I +      D W
Sbjct: 905  PGHKGFKPYCYTGAHTNHVADYITATREWNIECLQSDFSTPDVDNILKIPLSFLPVNDRW 964

Query: 181  IWHYSRNGEYSTKSGYKLEMNRNVHQASTSVNAHNVWWRKVWKMKIPSKIKLFIWKAYHG 236
            IWHY  +G+YS  SGY L  +      S+  +    WW+  WK+ +PSK+K+F WK    
Sbjct: 965  IWHYEDSGDYSVSSGYTLASSLGEEDLSSCSHTQETWWKSFWKLNLPSKVKIFGWKVIQS 1024

BLAST of Lcy08g009140 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 55.5 bits (132), Expect = 9.9e-08
Identity = 24/49 (48.98%), Postives = 33/49 (67.35%), Query Frame = 0

Query: 2  MATFWWGTTDARKKLHWKKWEFMCLPKE-MGGLNFRDLISFNRSLLAKQ 50
          M  FWW + + ++K+ W  W+ +C  KE  GGL FRDL  FN++LLAKQ
Sbjct: 25 MTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQ 73

BLAST of Lcy08g009140 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.2e-07
Identity = 20/50 (40.00%), Postives = 31/50 (62.00%), Query Frame = 0

Query: 1  MMATFWWGTTDARKKLHWKKWEFMCLPKEMGGLNFRDLISFNRSLLAKQV 51
          ++A FWW      K +HWK W+ +   K  GG+ F+D+ +FN +LL KQ+
Sbjct: 24 VLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIEAFNLALLGKQM 73

BLAST of Lcy08g009140 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.4e-07
Identity = 31/128 (24.22%), Postives = 55/128 (42.97%), Query Frame = 0

Query: 122 VWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCGSEMESIDHALCGCKRAK 181
           +WK+ +  KIK F+W+   G L TN  L    +D   +   C  E E+I H +  C   +
Sbjct: 37  IWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQ 96

Query: 182 EICDMMFPRVDVDIFVADNFA---------NRILRLADHLSMEVFEK--TCITFWAIWND 239
            +          +I + + +          NR+++L+   +    ++       W +W  
Sbjct: 97  SVWR------SANIIIGNQWGPPSSFEDNLNRLIQLSKTQTTNSLDRFLPFWIMWRLWKS 156

BLAST of Lcy08g009140 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 6.0e-05
Identity = 31/130 (23.85%), Postives = 50/130 (38.46%), Query Frame = 0

Query: 121 KVWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCGSEMESIDHALCGCKRA 180
           K+WK+K   KIK F+WK   G L T   L +  +        C  E E+  H    C  A
Sbjct: 17  KIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYA 76

Query: 181 KEI------------CDMMFPRVDVDIFVADNFANRILRLADHLSMEVFEKTCITFWAIW 239
           +++               +     +++ ++   ANR          ++F       W +W
Sbjct: 77  QQVWRASGIPHQELRTTGITMETKMELLLSSCLANR--------QPQLFNLAIWILWRLW 136

BLAST of Lcy08g009140 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 43.9 bits (102), Expect = 3.0e-04
Identity = 23/66 (34.85%), Postives = 32/66 (48.48%), Query Frame = 0

Query: 116 NVWWRKVWKMKIPSKIKLFIWKAYHGCLPTNYCLWKCGMDVLSLYSVCGSEMESIDHALC 175
           N W   +W +KI  KIKL IWKA +  LP    L    + +    + C  + E+I H L 
Sbjct: 3   NNWIGDIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRC-RDFETITHILF 62

Query: 176 GCKRAK 182
            C  A+
Sbjct: 63  NCPFAQ 67

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P932951.4e-0648.98Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
P0C2F64.5e-0548.89Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A803PYI04.2e-3829.63Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QHJ77.9e-3730.81Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DX305.1e-3626.52uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803Q1851.9e-3526.85Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NI873.3e-3525.00Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022158377.11.1e-3526.52uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_030505962.16.8e-3525.00uncharacterized protein LOC115720894 [Cannabis sativa][more]
XP_030509188.12.6e-3430.58uncharacterized protein LOC115723863 [Cannabis sativa][more]
XP_030496655.12.9e-3327.76uncharacterized protein LOC115712508 [Cannabis sativa][more]
XP_030479133.12.7e-3127.38uncharacterized protein LOC115696372 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
ATMG00310.19.9e-0848.98RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G29090.12.2e-0740.00Ribonuclease H-like superfamily protein [more]
AT2G02650.18.4e-0724.22Ribonuclease H-like superfamily protein [more]
AT3G25270.16.0e-0523.85Ribonuclease H-like superfamily protein [more]
AT3G26855.13.0e-0434.85RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 250..332
e-value: 6.2E-8
score: 32.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 244..336
e-value: 3.3E-8
score: 35.6
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 91..182
e-value: 1.6E-15
score: 57.6
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 62..327
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 62..327
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 247..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g009140.1Lcy08g009140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity