Lcy08g008690 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy08g008690
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr08: 16925699 .. 16926169 (+)
RNA-Seq Expression	Lcy08g008690
Synteny	Lcy08g008690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGACCCCCCATTCCGGCTGGGCGAAAATAAACGTCGATGCTTCTTGGAATGAGCGGTCGAATGCGGGAGGCGTTGGGTGGATCATCCGTGATTCGGTTGGATCTCCGATGGGCATGGGAGCTGCGAAAATTCAGAGGCGTTGGTCAGTGAAATCCCTAGAAGCAGAAGCTGTGTAAGAAGGAGTTGAGGCCTACCTCTCGGGCAGGAAGAACCAATTTCTTGGGGCTATGGCGGAATCAAACTCTTTGGATGTGGTGAAATCTCTAAACCACACCTGTTCGGATGTTTCCAAAACTTCAGTGATCTTAGAGGATGTGGATCGTCTCGCTGCAGATGTAGGCTCAATTGCCTTCGTGAAATGTTCGCGAGAATCGAACAAGGTAGCGCACACTCTTGCGCGATTAGCAGCTGGTTTTATCGGAGATCTCCCCGTCGTCCGTTGTAGAAGACGGTTTTTTTGGGCTTGA

mRNA sequence

ATGGAGACCCCCCATTCCGGCTGGGCGAAAATAAACGTCGATGCTTCTTGGAATGAGCGGTCGAATGCGGGAGGCGTTGGGTGGATCATCCGTGATTCGGTTGGATCTCCGATGGGCATGGGAGCTGCGAAAATTCAGAGGCGTTGGAAGAACCAATTTCTTGGGGCTATGGCGGAATCAAACTCTTTGGATGTGGTGAAATCTCTAAACCACACCTGTTCGGATGTTTCCAAAACTTCAGTGATCTTAGAGGATGTGGATCGTCTCGCTGCAGATGTAGGCTCAATTGCCTTCGTGAAATGTTCGCGAGAATCGAACAAGGTAGCGCACACTCTTGCGCGATTAGCAGCTGGTTTTATCGGAGATCTCCCCGTCGTCCGTTGTAGAAGACGGTTTTTTTGGGCTTGA

Coding sequence (CDS)

ATGGAGACCCCCCATTCCGGCTGGGCGAAAATAAACGTCGATGCTTCTTGGAATGAGCGGTCGAATGCGGGAGGCGTTGGGTGGATCATCCGTGATTCGGTTGGATCTCCGATGGGCATGGGAGCTGCGAAAATTCAGAGGCGTTGGAAGAACCAATTTCTTGGGGCTATGGCGGAATCAAACTCTTTGGATGTGGTGAAATCTCTAAACCACACCTGTTCGGATGTTTCCAAAACTTCAGTGATCTTAGAGGATGTGGATCGTCTCGCTGCAGATGTAGGCTCAATTGCCTTCGTGAAATGTTCGCGAGAATCGAACAAGGTAGCGCACACTCTTGCGCGATTAGCAGCTGGTTTTATCGGAGATCTCCCCGTCGTCCGTTGTAGAAGACGGTTTTTTTGGGCTTGA

Protein sequence

METPHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMAESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRESNKVAHTLARLAAGFIGDLPVVRCRRRFFWA

Homology

[Close]

BLAST of Lcy08g008690 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.9e-08
Identity = 41/131 (31.30%), Postives = 65/131 (49.62%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMA----- 63
           P S   K+N +A+W   +N GG+GWI+RD  G  +      I+      +L  MA     
Sbjct: 82  PTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGL 141

Query: 64  -------------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 117
                        ES+SL+ +  L+  C D ++   +LE++ ++  D+  ++    SRE+
Sbjct: 142 RAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREA 201

[Close]

BLAST of Lcy08g008690 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.0e-07
Identity = 42/139 (30.22%), Postives = 65/139 (46.76%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMA----- 63
           P S   K+N DA+W   +N GG+GWI+RD  G  +      I+      +L  MA     
Sbjct: 82  PTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGL 141

Query: 64  ---------------------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIA 117
                                ES+SL+ +  L+  C D ++   +LE++ ++  D+  ++
Sbjct: 142 RAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVS 201

[Close]

BLAST of Lcy08g008690 vs. ExPASy TrEMBL
Match: A0A498J3L0 (RNase H domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_031602 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 5.8e-07
Identity = 43/128 (33.59%), Postives = 61/128 (47.66%), Query Frame = 0


Query: 3   TPHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGA------ 62
           +P   W KIN+D + N +    GVG +IRD  G  +      I RR+     GA      
Sbjct: 4   SPTHRWVKINIDGALNVQDKLSGVGVVIRDWRGGHLVARGTTIPRRYIGTNFGAVFSEMA 63

Query: 63  --------MAESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRESNKV 117
                   + ES+ L  + +LN T SD+S    I +DV +LA+    + F+  SR  N V
Sbjct: 64  QQQGFQQVVVESDCLKAIATLNWTHSDLSAIGTIADDVLQLASTFPMVRFIHVSRLCNGV 123

[Close]

BLAST of Lcy08g008690 vs. ExPASy TrEMBL
Match: A0A6D2IMK4 (RNase H domain-containing protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS16361 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.3e-06
Identity = 40/141 (28.37%), Postives = 69/141 (48.94%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQR------------RWKN 63
           P +GW K NVD +W+ R +  GVGW++RD  G  + MGA +I +            RW  
Sbjct: 351 PPAGWLKCNVDGAWSHRGDHSGVGWVLRDDKGKVLWMGAKRIHKTQTVVEAELEALRWAA 410

Query: 64  QFLGAMA------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 123
           +F+ ++       ES+S + V+ ++    D  K   + +D+ +   ++ +   V  SR  
Sbjct: 411 EFVTSLRYQNIIFESDSKEAVEVISFE-DDWPKYRAVAQDLRKTLKNLCNYKIVFHSRAV 470

Query: 124 NKVAHTLARLAAGFIGDLPVV 127
           N VA  +AR    ++  +P V
Sbjct: 471 NMVADRIARETNTYVDYVPKV 490

[Close]

BLAST of Lcy08g008690 vs. ExPASy TrEMBL
Match: A0A498K7Q8 (Uncharacterized protein (Fragment) OS=Malus domestica OX=3750 GN=DVH24_030263 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.9e-06
Identity = 36/118 (30.51%), Postives = 61/118 (51.69%), Query Frame = 0


Query: 7   GWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKI---QRRWK-----NQFLGAMA 66
           G  K+NVD +WNE SN GGVG I+R+  G  + + + +I    + W        F   + 
Sbjct: 27  GVTKVNVDGAWNESSNLGGVGVIVRNDSGDFIAVTSVRITHDDQGWSVIGAAKGFQNFIL 86

Query: 67  ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRESNKVAHTLARLA 117
           E +SL +V +L  +        ++++D   L A +  +++    R++N +AH LAR A
Sbjct: 87  ECDSLKIVAALRDSSPFPFNVRIVVKDSRELMASITEVSYTHVRRQANGIAHRLARAA 144

[Close]

BLAST of Lcy08g008690 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 66.6 bits (161), Expect = 1.8e-07
Identity = 41/131 (31.30%), Postives = 65/131 (49.62%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMA----- 63
           P S   K+N +A+W   +N GG+GWI+RD  G  +      I+      +L  MA     
Sbjct: 82  PTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGL 141

Query: 64  -------------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 117
                        ES+SL+ +  L+  C D ++   +LE++ ++  D+  ++    SRE+
Sbjct: 142 RAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREA 201

[Close]

BLAST of Lcy08g008690 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 65.5 bits (158), Expect = 4.1e-07
Identity = 42/139 (30.22%), Postives = 65/139 (46.76%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMA----- 63
           P S   K+N DA+W   +N GG+GWI+RD  G  +      I+      +L  MA     
Sbjct: 82  PTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGL 141

Query: 64  ---------------------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIA 117
                                ES+SL+ +  L+  C D ++   +LE++ ++  D+  ++
Sbjct: 142 RAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVS 201

[Close]

BLAST of Lcy08g008690 vs. NCBI nr
Match: RXH89245.1 (hypothetical protein DVH24_031602 [Malus domestica])

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-06
Identity = 43/128 (33.59%), Postives = 61/128 (47.66%), Query Frame = 0


Query: 3   TPHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGA------ 62
           +P   W KIN+D + N +    GVG +IRD  G  +      I RR+     GA      
Sbjct: 4   SPTHRWVKINIDGALNVQDKLSGVGVVIRDWRGGHLVARGTTIPRRYIGTNFGAVFSEMA 63

Query: 63  --------MAESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRESNKV 117
                   + ES+ L  + +LN T SD+S    I +DV +LA+    + F+  SR  N V
Sbjct: 64  QQQGFQQVVVESDCLKAIATLNWTHSDLSAIGTIADDVLQLASTFPMVRFIHVSRLCNGV 123

[Close]

BLAST of Lcy08g008690 vs. NCBI nr
Match: XP_030969974.1 (uncharacterized protein LOC115990267 [Quercus lobata])

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-06
Identity = 44/131 (33.59%), Postives = 62/131 (47.33%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMA----- 63
           P  GW K NVD +  + SN  G+G ++RDS G  +     K+      + + A+A     
Sbjct: 15  PPQGWFKANVDGAIFKESNKAGIGVVVRDSQGWVLAALTEKVDGVQDAEVIEALAIRRAI 74

Query: 64  -------------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 117
                        ES+SL VVK++  T         I+EDV  L+  + S  F    RE+
Sbjct: 75  RFAIETSFNCVIIESDSLSVVKAIQDTAEPTCHIGNIIEDVKLLSKTMKSCEFHHTKREA 134

[Close]

BLAST of Lcy08g008690 vs. NCBI nr
Match: XP_030924618.1 (uncharacterized protein LOC115951582 [Quercus lobata])

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-06
Identity = 41/131 (31.30%), Postives = 64/131 (48.85%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQRRWKNQFLGAMA----- 63
           P  GW K NVD +  + +N  G+G ++RDS G  +     K++     + +GA+A     
Sbjct: 19  PPQGWLKANVDGAIFKETNKAGIGVVVRDSQGWVLATLTEKVEGVQDAEVIGALAIQRAI 78

Query: 64  -------------ESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 117
                        ES+SL VVK++ +T         I++DV  L+  + S  F    RE+
Sbjct: 79  RFAIETSFNCVIIESDSLSVVKAIQYTAESTCHIGNIIDDVKHLSKAMKSCEFHHTKREA 138

[Close]

BLAST of Lcy08g008690 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.2e-07
Identity = 39/135 (28.89%), Postives = 63/135 (46.67%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQR------------RW-- 63
           PH  W K N DA+WN  +   G+GW++R+  G    MGA  + +            RW  
Sbjct: 424 PHQ-WVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAV 483

Query: 64  ----KNQFLGAMAESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 121
               + Q+   + ES+S  +++ LN+     S    I +D+ RL +    + FV   RE 
Sbjct: 484 LSLSRFQYNYVIFESDSQVLIEILNNDEIWPSLKPTI-QDLQRLLSQFTEVKFVFIPREG 543

[Close]

BLAST of Lcy08g008690 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 39/137 (28.47%), Postives = 58/137 (42.34%), Query Frame = 0


Query: 2   ETPHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAKIQR------------RW 61
           + P   W K N DA+W   +   G+GWI+R+  G  + MGA  + R            RW
Sbjct: 138 KAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRW 197

Query: 62  ------KNQFLGAMAESNSLDVVKSLNHTCSDVSKT-SVILEDVDRLAADVGSIAFVKCS 120
                 +  +   + ES++  +V  LN    D   T    LED+ +L      + F    
Sbjct: 198 AVLTMSRFNYKRIIFESDAQALVNLLN--SDDFWPTLQPALEDIQQLLHHFEEVKFEFTP 257

[Close]

BLAST of Lcy08g008690 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.2e-04
Identity = 37/146 (25.34%), Postives = 60/146 (41.10%), Query Frame = 0


Query: 4   PHSGWAKINVDASWNERSNAGGVGWIIRDSVGSPMGMGAAK------------------I 63
           P + + K N DA ++ +      GWIIR+  G+P+  G+ K                  +
Sbjct: 329 PPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNPLEAETKALLAAL 388

Query: 64  QRRWKNQFLGAMAESNSLDVVKSLNHTCSDVSKTSVILEDVDRLAADVGSIAFVKCSRES 123
           Q+ W   +     E +   ++  +N   S  S  +  LED+   A    SI F    R+ 
Sbjct: 389 QQTWIRGYTQVFMEGDCQTLINLIN-GISFHSSLANHLEDISFWANKFASIQFGFIRRKG 448

Query: 124 NKVAHTLARLAAGF------IGDLPV 126
           NK+AH LA+    +       G LP+
Sbjct: 449 NKLAHVLAKYGCTYSTFYSGSGSLPI 473

The following BLAST results are available for this feature:

BLAST of Lcy08g008690 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy08g008690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	8.9e-08	31.30	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	2.0e-07	30.22	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A498J3L0	5.8e-07	33.59	RNase H domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_031602 PE=...	[more]
A0A6D2IMK4	1.3e-06	28.37	RNase H domain-containing protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_L...	[more]
A0A498K7Q8	2.9e-06	30.51	Uncharacterized protein (Fragment) OS=Malus domestica OX=3750 GN=DVH24_030263 PE...	[more]

BLAST of Lcy08g008690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143535.1	1.8e-07	31.30	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	4.1e-07	30.22	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
RXH89245.1	1.2e-06	33.59	hypothetical protein DVH24_031602 [Malus domestica]	[more]
XP_030969974.1	1.2e-06	33.59	uncharacterized protein LOC115990267 [Quercus lobata]	[more]
XP_030924618.1	1.6e-06	31.30	uncharacterized protein LOC115951582 [Quercus lobata]	[more]

BLAST of Lcy08g008690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	1.2e-07	28.89	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	1.4e-05	28.47	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G09510.1	1.2e-04	25.34	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 12..52 e-value: 6.2E-6 score: 26.0
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 2..117
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 2..117
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 11..114 e-value: 3.11248E-13 score: 60.0204
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 8..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy08g008690.1	Lcy08g008690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity