Lcy08g008510 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g008510
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr08: 15781509 .. 15782383 (-)
RNA-Seq ExpressionLcy08g008510
SyntenyLcy08g008510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAATAACCCTGGGTGTTATTTTTGCAGGAGATTGTGGGAATCGACTGGGCATGTTTTATGGAACTGCAAGCAGGTGAGAGATATATGGTTGGCCTTGTTCCCGAATCTTAAAGCTTTCTTTCTCAATTTCAGAGAAGGAGAAGAGACTGACATAATCTCGAAAGGATGATTAGAGAGCTCAATGAGGAAAACTTGGAGGAAGTGGCTATCACTCTATGGACTATTTGGAATTTTAGAAACAGATCAACAATCGCAAATGATAAGGCAAATTTCAAGACGTTTCTTAGGGAAATTAAAAAGAACATCAATGAACAAAGGAAAAGAATCAATCATAACCTGGTAGCGAGCTCCTCAGAGAATTAATGGAATCATGGATTATGATCTCCCCCCCCCCCCCCAAGCGGAACTCTAAAGCTGAATGTGGATGCGGATGCCTCATAGGACGATTCCCAAAAGAGAGGTGGTATTGGGTGGGTTGTTTGTGATTCCCTTGGATCTCCAAAAGGCATTAGCTGCAAAAGTTGGAACAGAGGATGGTCAATCAAATCAATGGAAAGTCTGGCGATTAAAGAAGGTTTGAAGGCTTACTTGGATTCAGAAGAAGTCAGAAGGGCTCCGATCATAGTGGAAGCAGACGCGACAGAAGTGATAAATGCTCTGAATTTTGTTTGCGAAGATCTCTCAGAAGCAAAGATTGTAATGGTTGACATCGAAGCTCTGGTGTCTAGGGCTGGAGTGATTTCTTTCGAAAAATGCCCTCGTGCATGTAATAGAAGCGCGCACTTGCTCGTGCGTTTGGCGGCTAGCTTTAGGCCGCCAAAATTCCTACCGTCCGATGTCGACAGAAGGAGGGTTGTTAGTTGTGTTTAA

mRNA sequence

ATGGACAATAACCCTGGGTGTTATTTTTGCAGGAGATTGTGGGAATCGACTGGGCATGTTTTATGGAACTGCAAGCAGGACGATTCCCAAAAGAGAGGTGGTATTGGGTGGGTTGTTTGTGATTCCCTTGGATCTCCAAAAGGCATTAGCTGCAAAAGTTGGAACAGAGGATGGTCAATCAAATCAATGGAAAGTCTGGCGATTAAAGAAGGTTTGAAGGCTTACTTGGATTCAGAAGAAGTCAGAAGGGCTCCGATCATAGTGGAAGCAGACGCGACAGAAGTGATAAATGCTCTGAATTTTGTTTGCGAAGATCTCTCAGAAGCAAAGATTGTAATGGTTGACATCGAAGCTCTGGTGTCTAGGGCTGGAGTGATTTCTTTCGAAAAATGCCCTCGTGCATGTAATAGAAGCGCGCACTTGCTCGTGCGTTTGGCGGCTAGCTTTAGGCCGCCAAAATTCCTACCGTCCGATGTCGACAGAAGGAGGGTTGTTAGTTGTGTTTAA

Coding sequence (CDS)

ATGGACAATAACCCTGGGTGTTATTTTTGCAGGAGATTGTGGGAATCGACTGGGCATGTTTTATGGAACTGCAAGCAGGACGATTCCCAAAAGAGAGGTGGTATTGGGTGGGTTGTTTGTGATTCCCTTGGATCTCCAAAAGGCATTAGCTGCAAAAGTTGGAACAGAGGATGGTCAATCAAATCAATGGAAAGTCTGGCGATTAAAGAAGGTTTGAAGGCTTACTTGGATTCAGAAGAAGTCAGAAGGGCTCCGATCATAGTGGAAGCAGACGCGACAGAAGTGATAAATGCTCTGAATTTTGTTTGCGAAGATCTCTCAGAAGCAAAGATTGTAATGGTTGACATCGAAGCTCTGGTGTCTAGGGCTGGAGTGATTTCTTTCGAAAAATGCCCTCGTGCATGTAATAGAAGCGCGCACTTGCTCGTGCGTTTGGCGGCTAGCTTTAGGCCGCCAAAATTCCTACCGTCCGATGTCGACAGAAGGAGGGTTGTTAGTTGTGTTTAA

Protein sequence

MDNNPGCYFCRRLWESTGHVLWNCKQDDSQKRGGIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYLDSEEVRRAPIIVEADATEVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCPRACNRSAHLLVRLAASFRPPKFLPSDVDRRRVVSCV
Homology
BLAST of Lcy08g008510 vs. ExPASy TrEMBL
Match: A0A803L9N8 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.0e-06
Identity = 38/128 (29.69%), Postives = 64/128 (50.00%), Query Frame = 0

Query: 31  KRGGIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYLDSEEVRRAPIIVEA 90
           +RGG+G VV D++G       KS N    I  +E++A+  G++A  D+   +   + V +
Sbjct: 147 RRGGLGMVVRDNIGDVMMTWGKSLNGTGIISEIEAVALLFGMRAVFDAGFRK---LEVSS 206

Query: 91  DATEVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCPRACNRSAHLLVRLAASFR 150
           D  +VI  LN    + S  ++++ DI +       + F  CPR CN+ AH +  L+ S  
Sbjct: 207 DCLQVIELLNGSKHENSPTQLIVTDILSFAYSFDFVCFSFCPRLCNKVAHSMANLSLSLS 266

Query: 151 PPKFLPSD 159
             +F   D
Sbjct: 267 EERFWMED 271

BLAST of Lcy08g008510 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.6e-04
Identity = 38/108 (35.19%), Postives = 56/108 (51.85%), Query Frame = 0

Query: 33   GGIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYLDSEEVRRAPIIVEADA 92
            GG+GWVV DS G      CK   RG S  +ME+ AI+E L A + S     + + VE+D+
Sbjct: 1082 GGVGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQS---GLSKLEVESDS 1141

Query: 93   TEVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCPRACNRSAH 141
             ++I  L          + ++ DI+ LV +     F   PR CN++AH
Sbjct: 1142 LQLIRMLGGEWNIDIAVETIVFDIKQLVCQFQHCVFLYTPRNCNKAAH 1186

BLAST of Lcy08g008510 vs. ExPASy TrEMBL
Match: A0A5P1EL44 (Uncharacterized protein OS=Asparagus officinalis OX=4686 GN=A4U43_C06F7380 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 5.7e-04
Identity = 48/147 (32.65%), Postives = 70/147 (47.62%), Query Frame = 0

Query: 12   RLWE--STGHVLWNCKQDDSQKRG--GIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLA 71
            R+W   + G V  N        RG  G G VV D  GS  G+         S  + E+LA
Sbjct: 904  RIWRKPTNGFVKLNVDGALDIHRGIYGTGAVVRDERGSCVGVLATPGTGALSPLTCEALA 963

Query: 72   IKEGLKAYLDSEEVRRAPIIVEADATEVINALNFVCEDLSEAKIVMVDIEALVSRAGVIS 131
            +  GL   +   +V    +++E D+  VINALN   EDLS    ++ + + L+S     S
Sbjct: 964  LVNGLHFCV---QVGFKYVVIEGDSLAVINALNGSEEDLSMEGGLIDEAKFLMSFFISCS 1023

Query: 132  FEKCPRACNRSAHLLVRLAASFRPPKF 155
            ++  PR CN++AH + R A S   P F
Sbjct: 1024 WDFVPRVCNQAAHCVARRALSLPYPTF 1047

BLAST of Lcy08g008510 vs. ExPASy TrEMBL
Match: A0A7C9A7V4 (Uncharacterized protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 9.7e-04
Identity = 38/117 (32.48%), Postives = 60/117 (51.28%), Query Frame = 0

Query: 34  GIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYLDSEEVRRAPIIVEADAT 93
           G+G VV D  G  K ++C+S+   W ++++E+ A+  GL+      +     + +E D+ 
Sbjct: 311 GVGVVVRDKNGDVKIMACRSFRANWEVETVEAYAVLYGLQI---CWQEGFHTLELETDSK 370

Query: 94  EVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCPRACNRSAHLLVRLAASFR 151
           +V +ALN   + L+     + D   L +R  VISF    R  NR AH L RLA   R
Sbjct: 371 QVADALNGKKDFLNYTSTFIHDALNLGTRIPVISFSFVSRVTNRVAHELARLALRLR 424

BLAST of Lcy08g008510 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 61.6 bits (148), Expect = 7.4e-06
Identity = 41/104 (39.42%), Postives = 54/104 (51.92%), Query Frame = 0

Query: 32  RGGIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYL---DSEEVRRAPIIV 91
           +GG+ WV+ DSLGS   I CK   R W +K +E  A+ EGLK YL   ++      PII 
Sbjct: 20  KGGVSWVIHDSLGSSICIECKELRRKWLVKMLEGKAMIEGLKTYLLIRETVSYSHRPIIF 79

Query: 92  EADATEVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCP 133
                E+   LN    DL E   V+ +I  L   AG+ISF + P
Sbjct: 80  ----LELARILNNTHMDLIELSNVVDEIFDLELLAGIISFFQVP 119

BLAST of Lcy08g008510 vs. NCBI nr
Match: XP_021763631.1 (uncharacterized protein LOC110728264 [Chenopodium quinoa])

HSP 1 Score: 60.5 bits (145), Expect = 1.6e-05
Identity = 38/128 (29.69%), Postives = 64/128 (50.00%), Query Frame = 0

Query: 31  KRGGIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYLDSEEVRRAPIIVEA 90
           +RGG+G VV D++G       KS N    I  +E++A+  G++A  D+   +   + V +
Sbjct: 147 RRGGLGMVVRDNIGDVMMTWGKSLNGTGIISEIEAVALLFGMRAVFDAGFRK---LEVSS 206

Query: 91  DATEVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCPRACNRSAHLLVRLAASFR 150
           D  +VI  LN    + S  ++++ DI +       + F  CPR CN+ AH +  L+ S  
Sbjct: 207 DCLQVIELLNGSKHENSPTQLIVTDILSFAYSFDFVCFSFCPRLCNKVAHSMANLSLSLS 266

Query: 151 PPKFLPSD 159
             +F   D
Sbjct: 267 EERFWMED 271

BLAST of Lcy08g008510 vs. NCBI nr
Match: CAB4279811.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 55.5 bits (132), Expect = 5.3e-04
Identity = 38/108 (35.19%), Postives = 56/108 (51.85%), Query Frame = 0

Query: 33   GGIGWVVCDSLGSPKGISCKSWNRGWSIKSMESLAIKEGLKAYLDSEEVRRAPIIVEADA 92
            GG+GWVV DS G      CK   RG S  +ME+ AI+E L A + S     + + VE+D+
Sbjct: 1082 GGVGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQS---GLSKLEVESDS 1141

Query: 93   TEVINALNFVCEDLSEAKIVMVDIEALVSRAGVISFEKCPRACNRSAH 141
             ++I  L          + ++ DI+ LV +     F   PR CN++AH
Sbjct: 1142 LQLIRMLGGEWNIDIAVETIVFDIKQLVCQFQHCVFLYTPRNCNKAAH 1186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A803L9N88.0e-0629.69Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A6J5UV012.6e-0435.19Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A5P1EL445.7e-0432.65Uncharacterized protein OS=Asparagus officinalis OX=4686 GN=A4U43_C06F7380 PE=4 ... [more]
A0A7C9A7V49.7e-0432.48Uncharacterized protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAG6599977.17.4e-0639.42hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_021763631.11.6e-0529.69uncharacterized protein LOC110728264 [Chenopodium quinoa][more]
CAB4279811.15.3e-0435.19unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 23..150
e-value: 1.4E-7
score: 33.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 28..145
e-value: 1.2E-13
score: 50.9
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 27..144
e-value: 3.8588E-13
score: 60.7908
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 30..151

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g008510.1Lcy08g008510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity