Lcy08g007760 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g007760
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionaspartyl protease family protein 2
LocationChr08: 10533178 .. 10534870 (-)
RNA-Seq ExpressionLcy08g007760
SyntenyLcy08g007760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCACCACCAAAAAATATCCTCCTCTTCTTCCTCCTCCTCTCCTTCTCCGCCGCATCGGAGTTTCAAACCCTAATCCTCCGCCCTCTCCCTAATCCCTCTCCCCTTTCCTTTCCACAATCCCAACCCCAATTCGACTCCGAAGCCCTCGATTCCACCGCCGCCCTCACGCTTGACCTCCACCACTTGGACTCACTCTCCCTCAACAAAACCCCCACCGATCTCTTCAACCTCAGACTCCACCGCGATGCCCTCCGCGTCCAGTCGCTGACCGCTCTGGCCGCCGCCCGGACCCGGACCCCACTCCCACGCGCCGGCTTCAGCAGCTCCGTCATCTCCGGCCTCGCCCAAGGCAGCGGCGAGTACTTCACCCGCCTCGGCGTCGGAACCCCTCCTAGATACCTTTACATGGTCCTCGACACTGGAAGCGACGTCGTTTGGCTCCAATGCTCCCCTTGCCGCAAATGCTACACCCAATCCGACCCCATTTTCAACCCCTTCAAATCCAAATCCTTCGCCGGAATCCCCTGCTCCTCCCCTCTCTGCCGCCGCCTCGACTCCTCCGGCTGCAGCACCCGCCGCCACACCTGCCTCTACCAAGTCTCCTACGGCGACGGCTCCTTCACCACCGGCGACTTCGCCACCGAAACGCTCACGTTTCGCGGCAATAAAATCGCCAAAGTCGCCCTCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGCGCCGCCGGTTTGTTGGGCCTCGGCCGTGGCCGCTTGTCTTTCCCTTCCCAAACTGGAATCCGGTTCAATCACAAATTCTCCTATTGCCTCGTCGACCGGTCCGCTTCCTCCAGACCGTCGTCCATGGTTTTCGGCGATGCAGCGATTTCCCGGCTCGCCCGGTTCACTCCTCTGATTAGAAACCCAAAACTGGAAACGTTTTATTATGTCGAACTCATCGGAATCAGCGTCGGCGGAGTCCGCGTCCGCGGCGTCTCCGCTTCACTCTTCAAGCTCGATCCGGCCGGCAACGGCGGCGTCATCATCGATTCAGGTACGTCGGTAACCCGGTTGACCCGACCCGCTTACACGGCTCTTCGCGACGCGTTCAGGGCCGGAGCGGCCCATTTGAAAAGGGGTCCAGAGTTTTCGCTGTTCGATACTTGTTACGACTTGTCGGGTCAGTCCGCCGTGAAGGTTCCGACGGTGGTGCTGCATTTCCGGGGAGCCGACATGTCGTTGCCGGCGACGAATTATTTGATTCCGGTGGACGACAGTGGGAGCTTTTGCTTTGCGTTTGCGGGTACAATGTCCGGTTTGTCGATTATTGGGAATATTCAACAGCAGGGGTTCCGGGTTGTGTACGATTTGGCGGGTTCTCGGATCGGGTTTGCTCCACGTGGGTGCACGTGATCTCTGATCCTTAGATGAGATGAGATTTGTCTTTTATAGGGACAGAAACGGAATAACGAAAAAGAAAAGAAAAGAAAAGAAAAGAAAAATAGGATTTTTCGTGATGAATTTGTCTTCGTAAGGGTTGTTTTTGTGGTGTATTTGTATTTATTATCTTTTTAAAGTAAGTAAAGCCTCTAACTTAGAGGTGATTTGGTTTGTGTTAAAAGGGAGAGATTTGGACATCTGGTCCTTTTTATCATAGTGGAATACTTTTAAATCAATTAAAGAATCAAAATTTATCTGTGTTAAT

mRNA sequence

ATGGAATCACCACCAAAAAATATCCTCCTCTTCTTCCTCCTCCTCTCCTTCTCCGCCGCATCGGAGTTTCAAACCCTAATCCTCCGCCCTCTCCCTAATCCCTCTCCCCTTTCCTTTCCACAATCCCAACCCCAATTCGACTCCGAAGCCCTCGATTCCACCGCCGCCCTCACGCTTGACCTCCACCACTTGGACTCACTCTCCCTCAACAAAACCCCCACCGATCTCTTCAACCTCAGACTCCACCGCGATGCCCTCCGCGTCCAGTCGCTGACCGCTCTGGCCGCCGCCCGGACCCGGACCCCACTCCCACGCGCCGGCTTCAGCAGCTCCGTCATCTCCGGCCTCGCCCAAGGCAGCGGCGAGTACTTCACCCGCCTCGGCGTCGGAACCCCTCCTAGATACCTTTACATGGTCCTCGACACTGGAAGCGACGTCGTTTGGCTCCAATGCTCCCCTTGCCGCAAATGCTACACCCAATCCGACCCCATTTTCAACCCCTTCAAATCCAAATCCTTCGCCGGAATCCCCTGCTCCTCCCCTCTCTGCCGCCGCCTCGACTCCTCCGGCTGCAGCACCCGCCGCCACACCTGCCTCTACCAAGTCTCCTACGGCGACGGCTCCTTCACCACCGGCGACTTCGCCACCGAAACGCTCACGTTTCGCGGCAATAAAATCGCCAAAGTCGCCCTCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGCGCCGCCGGTTTGTTGGGCCTCGGCCGTGGCCGCTTGTCTTTCCCTTCCCAAACTGGAATCCGGTTCAATCACAAATTCTCCTATTGCCTCGTCGACCGGTCCGCTTCCTCCAGACCGTCGTCCATGGTTTTCGGCGATGCAGCGATTTCCCGGCTCGCCCGGTTCACTCCTCTGATTAGAAACCCAAAACTGGAAACGTTTTATTATGTCGAACTCATCGGAATCAGCGTCGGCGGAGTCCGCGTCCGCGGCGTCTCCGCTTCACTCTTCAAGCTCGATCCGGCCGGCAACGGCGGCGTCATCATCGATTCAGGTACGTCGGTAACCCGGTTGACCCGACCCGCTTACACGGCTCTTCGCGACGCGTTCAGGGCCGGAGCGGCCCATTTGAAAAGGGGTCCAGAGTTTTCGCTGTTCGATACTTGTTACGACTTGTCGGGTCAGTCCGCCGTGAAGGTTCCGACGGTGGTGCTGCATTTCCGGGGAGCCGACATGTCGTTGCCGGCGACGAATTATTTGATTCCGGTGGACGACAGTGGGAGCTTTTGCTTTGCGTTTGCGGGTACAATGTCCGGTTTGTCGATTATTGGGAATATTCAACAGCAGGGGTTCCGGGTTGTGTACGATTTGGCGGGTTCTCGGATCGGGTTTGCTCCACGTGGGTGCACGTGATCTCTGATCCTTAGATGAGATGAGATTTGTCTTTTATAGGGACAGAAACGGAATAACGAAAAAGAAAAGAAAAGAAAAGAAAAGAAAAATAGGATTTTTCGTGATGAATTTGTCTTCGTAAGGGTTGTTTTTGTGGTGTATTTGTATTTATTATCTTTTTAAAGTAAGTAAAGCCTCTAACTTAGAGGTGATTTGGTTTGTGTTAAAAGGGAGAGATTTGGACATCTGGTCCTTTTTATCATAGTGGAATACTTTTAAATCAATTAAAGAATCAAAATTTATCTGTGTTAAT

Coding sequence (CDS)

ATGGAATCACCACCAAAAAATATCCTCCTCTTCTTCCTCCTCCTCTCCTTCTCCGCCGCATCGGAGTTTCAAACCCTAATCCTCCGCCCTCTCCCTAATCCCTCTCCCCTTTCCTTTCCACAATCCCAACCCCAATTCGACTCCGAAGCCCTCGATTCCACCGCCGCCCTCACGCTTGACCTCCACCACTTGGACTCACTCTCCCTCAACAAAACCCCCACCGATCTCTTCAACCTCAGACTCCACCGCGATGCCCTCCGCGTCCAGTCGCTGACCGCTCTGGCCGCCGCCCGGACCCGGACCCCACTCCCACGCGCCGGCTTCAGCAGCTCCGTCATCTCCGGCCTCGCCCAAGGCAGCGGCGAGTACTTCACCCGCCTCGGCGTCGGAACCCCTCCTAGATACCTTTACATGGTCCTCGACACTGGAAGCGACGTCGTTTGGCTCCAATGCTCCCCTTGCCGCAAATGCTACACCCAATCCGACCCCATTTTCAACCCCTTCAAATCCAAATCCTTCGCCGGAATCCCCTGCTCCTCCCCTCTCTGCCGCCGCCTCGACTCCTCCGGCTGCAGCACCCGCCGCCACACCTGCCTCTACCAAGTCTCCTACGGCGACGGCTCCTTCACCACCGGCGACTTCGCCACCGAAACGCTCACGTTTCGCGGCAATAAAATCGCCAAAGTCGCCCTCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGCGCCGCCGGTTTGTTGGGCCTCGGCCGTGGCCGCTTGTCTTTCCCTTCCCAAACTGGAATCCGGTTCAATCACAAATTCTCCTATTGCCTCGTCGACCGGTCCGCTTCCTCCAGACCGTCGTCCATGGTTTTCGGCGATGCAGCGATTTCCCGGCTCGCCCGGTTCACTCCTCTGATTAGAAACCCAAAACTGGAAACGTTTTATTATGTCGAACTCATCGGAATCAGCGTCGGCGGAGTCCGCGTCCGCGGCGTCTCCGCTTCACTCTTCAAGCTCGATCCGGCCGGCAACGGCGGCGTCATCATCGATTCAGGTACGTCGGTAACCCGGTTGACCCGACCCGCTTACACGGCTCTTCGCGACGCGTTCAGGGCCGGAGCGGCCCATTTGAAAAGGGGTCCAGAGTTTTCGCTGTTCGATACTTGTTACGACTTGTCGGGTCAGTCCGCCGTGAAGGTTCCGACGGTGGTGCTGCATTTCCGGGGAGCCGACATGTCGTTGCCGGCGACGAATTATTTGATTCCGGTGGACGACAGTGGGAGCTTTTGCTTTGCGTTTGCGGGTACAATGTCCGGTTTGTCGATTATTGGGAATATTCAACAGCAGGGGTTCCGGGTTGTGTACGATTTGGCGGGTTCTCGGATCGGGTTTGCTCCACGTGGGTGCACGTGA

Protein sequence

MESPPKNILLFFLLLSFSAASEFQTLILRPLPNPSPLSFPQSQPQFDSEALDSTAALTLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Homology
BLAST of Lcy08g007760 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.3e-193
Identity = 345/477 (72.33%), Postives = 396/477 (83.02%), Query Frame = 0

Query: 7   NILLFFLLL-SFSAASEFQTLI--LRPLPNPSPLSFPQSQPQFDSEAL-----------D 66
           ++  FFL L SFS+   FQTL      LP  SP+SF   QP  DSE+L           +
Sbjct: 11  SLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSF---QPDSDSESLLESEFESGSDSE 70

Query: 67  STAALTLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAA---ARTRTPLPR-AGF 126
           S++++TL+L H+D+LS NKTP +LF+ RL RD+ RV+S+  LAA    R  T  PR  GF
Sbjct: 71  SSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGF 130

Query: 127 SSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPF 186
           SSSV+SGL+QGSGEYFTRLGVGTP RY+YMVLDTGSD+VWLQC+PCR+CY+QSDPIF+P 
Sbjct: 131 SSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPR 190

Query: 187 KSKSFAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAK 246
           KSK++A IPCSSP CRRLDS+GC+TRR TCLYQVSYGDGSFT GDF+TETLTFR N++  
Sbjct: 191 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKG 250

Query: 247 VALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFG 306
           VALGCGHDNEGLFVGAAGLLGLG+G+LSFP QTG RFN KFSYCLVDRSASS+PSS+VFG
Sbjct: 251 VALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFG 310

Query: 307 DAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSG 366
           +AA+SR+ARFTPL+ NPKL+TFYYV L+GISVGG RV GV+ASLFKLD  GNGGVIIDSG
Sbjct: 311 NAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSG 370

Query: 367 TSVTRLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM 426
           TSVTRL RPAY A+RDAFR GA  LKR P+FSLFDTC+DLS  + VKVPTVVLHFRGAD+
Sbjct: 371 TSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADV 430

Query: 427 SLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 466
           SLPATNYLIPVD +G FCFAFAGTM GLSIIGNIQQQGFRVVYDLA SR+GFAP GC
Sbjct: 431 SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Lcy08g007760 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.6e-112
Identity = 235/484 (48.55%), Postives = 300/484 (61.98%), Query Frame = 0

Query: 1   MESPPKNILLFFLLLSFSAASEFQTLILRPLPNPSPLSFPQSQPQFDSEALDSTAALTLD 60
           + +PPK  +L  +    S+  + QT IL   P  S L+  + +   D    +S++ L+L+
Sbjct: 29  LSTPPKTNVLDVV----SSLQQTQT-ILSLDPTRSSLTTTKPESLSDPVFFNSSSPLSLE 88

Query: 61  LHHLDSL--SLNKTPTDLFNLRLHRDALRVQSLTALAAARTR--------------TPLP 120
           LH  D+   S +K    L   RL RD+ RV  + A                     T   
Sbjct: 89  LHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQ 148

Query: 121 RAGFSSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPI 180
               ++ V+SG +QGSGEYF+R+GVGTP + +Y+VLDTGSDV W+QC PC  CY QSDP+
Sbjct: 149 TEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPV 208

Query: 181 FNPFKSKSFAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTF-RG 240
           FNP  S ++  + CS+P C  L++S C  R + CLYQVSYGDGSFT G+ AT+T+TF   
Sbjct: 209 FNPTSSSTYKSLTCSAPQCSLLETSAC--RSNKCLYQVSYGDGSFTVGELATDTVTFGNS 268

Query: 241 NKIAKVALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPS 300
            KI  VALGCGHDNEGLF GAAGLLGLG G LS  +Q        FSYCLVDR  S + S
Sbjct: 269 GKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSS 328

Query: 301 SMVFGDAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGV 360
           S+ F    +       PL+RN K++TFYYV L G SVGG +V  +  ++F +D +G+GGV
Sbjct: 329 SLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKV-VLPDAIFDVDASGSGGV 388

Query: 361 IIDSGTSVTRLTRPAYTALRDAFRAGAAHLKRG-PEFSLFDTCYDLSGQSAVKVPTVVLH 420
           I+D GT+VTRL   AY +LRDAF     +LK+G    SLFDTCYD S  S VKVPTV  H
Sbjct: 389 ILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFH 448

Query: 421 FRGA-DMSLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFA 466
           F G   + LPA NYLIPVDDSG+FCFAFA T S LSIIGN+QQQG R+ YDL+ + IG +
Sbjct: 449 FTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLS 500

BLAST of Lcy08g007760 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-107
Identity = 214/474 (45.15%), Postives = 296/474 (62.45%), Query Frame = 0

Query: 9   LLFFLL-----LSFSAASEFQTLILRPLPNPSPLSFPQSQPQFDSEAL--DSTAALTLDL 68
           L FF L     LS S++  F    +  +  P PL+   + P F++     +S++  TL L
Sbjct: 5   LFFFFLHLHLHLSSSSSISFPDFQIIDVLQP-PLTVTATLPDFNNTHFSDESSSKYTLRL 64

Query: 69  HHLDSLS--LNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRA-------GFSSSV 128
            H D       +      + R+ RD  RV ++    + +    +P +        F S +
Sbjct: 65  LHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV---IPSSDSRYEVNDFGSDI 124

Query: 129 ISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKS 188
           +SG+ QGSGEYF R+GVG+PPR  YMV+D+GSD+VW+QC PC+ CY QSDP+F+P KS S
Sbjct: 125 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGS 184

Query: 189 FAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALG 248
           + G+ C S +C R+++SGC +    C Y+V YGDGS+T G  A ETLTF    +  VA+G
Sbjct: 185 YTGVSCGSSVCDRIENSGCHS--GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMG 244

Query: 249 CGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAI 308
           CGH N G+F+GAAGLLG+G G +SF  Q   +    F YCLV R   S   S+VFG  A+
Sbjct: 245 CGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREAL 304

Query: 309 SRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVT 368
              A + PL+RNP+  +FYYV L G+ VGGVR+  +   +F L   G+GGV++D+GT+VT
Sbjct: 305 PVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRI-PLPDGVFDLTETGDGGVVMDTGTAVT 364

Query: 369 RLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHF-RGADMSLP 428
           RL   AY A RD F++  A+L R    S+FDTCYDLSG  +V+VPTV  +F  G  ++LP
Sbjct: 365 RLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLP 424

Query: 429 ATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 466
           A N+L+PVDDSG++CFAFA + +GLSIIGNIQQ+G +V +D A   +GF P  C
Sbjct: 425 ARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Lcy08g007760 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.3e-74
Identity = 168/410 (40.98%), Postives = 223/410 (54.39%), Query Frame = 0

Query: 61  LHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLAQGS 120
           L H+DS   N T   L    + R + R+Q L A+               S V + +  G 
Sbjct: 45  LEHVDS-GKNLTKFQLLERAIERGSRRLQRLEAMLNG-----------PSGVETSVYAGD 104

Query: 121 GEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIPCSS 180
           GEY   L +GTP +    ++DTGSD++W QC PC +C+ QS PIFNP  S SF+ +PCSS
Sbjct: 105 GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSS 164

Query: 181 PLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGL 240
            LC+ L S  CS   + C Y   YGDGS T G   TETLTF    I  +  GCG +N+G 
Sbjct: 165 QLCQALSSPTCS--NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGF 224

Query: 241 FVG-AAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARF- 300
             G  AGL+G+GRG LS PSQ  +    KFSYC+     SS PS+++ G  A S  A   
Sbjct: 225 GQGNGAGLVGMGRGPLSLPSQLDVT---KFSYCMTP-IGSSTPSNLLLGSLANSVTAGSP 284

Query: 301 -TPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDP-AGNGGVIIDSGTSVTRLTR 360
            T LI++ ++ TFYY+ L G+SVG  R+  +  S F L+   G GG+IIDSGT++T    
Sbjct: 285 NTTLIQSSQIPTFYYITLNGLSVGSTRL-PIDPSAFALNSNNGTGGIIIDSGTTLTYFVN 344

Query: 361 PAYTALRDAFRAGAAHLKRGPEFSLFDTCYDL-SGQSAVKVPTVVLHFRGADMSLPATNY 420
            AY ++R  F +           S FD C+   S  S +++PT V+HF G D+ LP+ NY
Sbjct: 345 NAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENY 404

Query: 421 LIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 466
            I    +G  C A   +  G+SI GNIQQQ   VVYD   S + FA   C
Sbjct: 405 FIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Lcy08g007760 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.1e-73
Identity = 160/395 (40.51%), Postives = 216/395 (54.68%), Query Frame = 0

Query: 81  LHRDALRVQSLTALAAARTRTPLPRAGFSSSVIS--GLAQGSGEYFTRLGVGTPPRYLYM 140
           L  D  RV S+ +  + +  T       S+ + +  G   GSG Y   +G+GTP   L +
Sbjct: 88  LRLDQARVNSIHSKLSKKLATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSL 147

Query: 141 VLDTGSDVVWLQCSPC-RKCYTQSDPIFNPFKSKSFAGIPCSSPLCRRLDS---SGCSTR 200
           + DTGSD+ W QC PC R CY Q +PIFNP KS S+  + CSS  C  L S   +  S  
Sbjct: 148 IFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCS 207

Query: 201 RHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAK-VALGCGHDNEGLFVGAAGLLGLGRG 260
              C+Y + YGD SF+ G  A E  T   + +   V  GCG +N+GLF G AGLLGLGR 
Sbjct: 208 ASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRD 267

Query: 261 RLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARFTPLIRNPKLETFYYV 320
           +LSFPSQT   +N  FSYCL   S++S    + FG A ISR  +FTP+       +FY +
Sbjct: 268 KLSFPSQTATAYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFYGL 327

Query: 321 ELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGAAHL 380
            ++ I+VGG ++  + +++F        G +IDSGT +TRL   AY ALR +F+A  +  
Sbjct: 328 NIVAITVGGQKL-PIPSTVF-----STPGALIDSGTVITRLPPKAYAALRSSFKAKMSKY 387

Query: 381 KRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLIPVDDSGSFCFAFAGTM 440
                 S+ DTC+DLSG   V +P V   F G  +    +  +  V      C AFAG  
Sbjct: 388 PTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNS 447

Query: 441 --SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
             S  +I GN+QQQ   VVYD AG R+GFAP GC+
Sbjct: 448 DDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of Lcy08g007760 vs. ExPASy TrEMBL
Match: A0A6J1KW81 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111498134 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.5e-245
Identity = 430/469 (91.68%), Postives = 447/469 (95.31%), Query Frame = 0

Query: 1   MESPPKNILLFFLLLS--FSAASEFQTLILRPLPNPSPLSFPQSQPQFDS-EALDSTAAL 60
           MESPP+N+L FF   +   SAASEFQTL LR LP PSPLSFPQS  QFD+ E L+STAAL
Sbjct: 1   MESPPRNLLFFFFFXAAVASAASEFQTLTLRRLPIPSPLSFPQS--QFDTQETLESTAAL 60

Query: 61  TLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLA 120
           T++LHHLDSLS NKTP+DLFNLRLHRDALRV SLT+L AAR+RTPL RAGFSSSVISGLA
Sbjct: 61  TVELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLA 120

Query: 121 QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIP 180
           QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGIP
Sbjct: 121 QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIP 180

Query: 181 CSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN 240
           CSSPLCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN
Sbjct: 181 CSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN 240

Query: 241 EGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLAR 300
           EGLFVGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLAR
Sbjct: 241 EGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLAR 300

Query: 301 FTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRP 360
           FTPLI NPKLETFYYVELIG SVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTRP
Sbjct: 301 FTPLILNPKLETFYYVELIGFSVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRP 360

Query: 361 AYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLI 420
           AYTALRDAFRAGA+HLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYLI
Sbjct: 361 AYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLI 420

Query: 421 PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467

BLAST of Lcy08g007760 vs. ExPASy TrEMBL
Match: A0A6J1H7E0 (aspartyl protease family protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111460848 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.2e-242
Identity = 427/470 (90.85%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MESPPKNILLFFLLL---SFSAASEFQTLILRPLPNPSPLSFPQSQPQFDS-EALDSTAA 60
           MESPP+ +L FF        SAASEFQTL LR LP PSPL FP  QPQFD+ E L+STAA
Sbjct: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFP--QPQFDTQETLESTAA 60

Query: 61  LTLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGL 120
           LT++LHHLDSLS NKTP+DLFNLRLHRDALRV SLT+L AAR+RTPL RAGFSSSVISGL
Sbjct: 61  LTVELHHLDSLSPNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGL 120

Query: 121 AQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGI 180
           AQGSGEYFTRLGVGTP RY+YMVLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGI
Sbjct: 121 AQGSGEYFTRLGVGTPSRYIYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGI 180

Query: 181 PCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD 240
           PCSSPLCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD
Sbjct: 181 PCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD 240

Query: 241 NEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLA 300
           NEGLFVGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLA
Sbjct: 241 NEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLA 300

Query: 301 RFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTR 360
           RFTPLI NPKLETFYYVELIGISVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTR
Sbjct: 301 RFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTR 360

Query: 361 PAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYL 420
           PAYTALRDAFRAGA+HLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYL
Sbjct: 361 PAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYL 420

Query: 421 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 468

BLAST of Lcy08g007760 vs. ExPASy TrEMBL
Match: A0A1S3CHC4 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103500464 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.0e-228
Identity = 412/464 (88.79%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 6   KNILLFFLLLSFSAASEFQTLILRPLPNPSPLS-FPQSQPQFDSEALDST--AALTLDLH 65
           K +LLF+ +   SAASEFQTL LR LP PSPLS FP      DSE+L S+  AALTLDLH
Sbjct: 8   KYLLLFYFI--SSAASEFQTLTLRSLPTPSPLSLFP------DSESLQSSPAAALTLDLH 67

Query: 66  HLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLAQGSGE 125
           HLDSLSLNKTPTDLFNLRLHRDALRV +LT+ AA          GFSSSVISGLAQGSGE
Sbjct: 68  HLDSLSLNKTPTDLFNLRLHRDALRVHALTSRAA---------PGFSSSVISGLAQGSGE 127

Query: 126 YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIPCSSPL 185
           YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCY+QSDPIFNP+KSKSFAGIPCSSPL
Sbjct: 128 YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPYKSKSFAGIPCSSPL 187

Query: 186 CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFV 245
           CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH NEGLFV
Sbjct: 188 CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHHNEGLFV 247

Query: 246 GAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARFTPLI 305
           GAAGLLGLGRGRLSFPSQTGIRFN KFSYCLVDRSASS+PSSMVFGDAAISRLARFTPLI
Sbjct: 248 GAAGLLGLGRGRLSFPSQTGIRFNRKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLI 307

Query: 306 RNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTAL 365
           RNPKL+TFYYVELIGISVGGVRVRGV  SLFKLD AGNGGVIIDSGTSVTRLTRPAYTAL
Sbjct: 308 RNPKLDTFYYVELIGISVGGVRVRGVYPSLFKLDSAGNGGVIIDSGTSVTRLTRPAYTAL 367

Query: 366 RDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLIPVDDS 425
           RDAFRAGA HLKRGPEFSLFDTCYDLSGQS+VKVPTVVLHFRGADM LPATNYLIPVD++
Sbjct: 368 RDAFRAGARHLKRGPEFSLFDTCYDLSGQSSVKVPTVVLHFRGADMPLPATNYLIPVDEN 427

Query: 426 GSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           GSFCFAFAGT+SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 428 GSFCFAFAGTISGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 454

BLAST of Lcy08g007760 vs. ExPASy TrEMBL
Match: A0A5A7U8Z2 (Aspartyl protease family protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold673G001170 PE=3 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.9e-228
Identity = 411/464 (88.58%), Postives = 426/464 (91.81%), Query Frame = 0

Query: 6   KNILLFFLLLSFSAASEFQTLILRPLPNPSPLS-FPQSQPQFDSEALDST--AALTLDLH 65
           K +LLF+ +   SAASEFQTL LR LP PSPL  FP      DSE+L S+  AALTLDLH
Sbjct: 8   KYLLLFYFI--SSAASEFQTLTLRSLPTPSPLPLFP------DSESLQSSPAAALTLDLH 67

Query: 66  HLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLAQGSGE 125
           HLDSLSLNKTPTDLFNLRLHRDALRV +LT+ AA          GFSSSVISGLAQGSGE
Sbjct: 68  HLDSLSLNKTPTDLFNLRLHRDALRVHALTSRAA---------PGFSSSVISGLAQGSGE 127

Query: 126 YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIPCSSPL 185
           YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCY+QSDPIFNP+KSKSFAGIPCSSPL
Sbjct: 128 YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPYKSKSFAGIPCSSPL 187

Query: 186 CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFV 245
           CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH NEGLFV
Sbjct: 188 CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHHNEGLFV 247

Query: 246 GAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARFTPLI 305
           GAAGLLGLGRGRLSFPSQTGIRFN KFSYCLVDRSASS+PSSMVFGDAAISRLARFTPLI
Sbjct: 248 GAAGLLGLGRGRLSFPSQTGIRFNRKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLI 307

Query: 306 RNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTAL 365
           RNPKL+TFYYVELIGISVGGVRVRGV  SLFKLD AGNGGVIIDSGTSVTRLTRPAYTAL
Sbjct: 308 RNPKLDTFYYVELIGISVGGVRVRGVYPSLFKLDSAGNGGVIIDSGTSVTRLTRPAYTAL 367

Query: 366 RDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLIPVDDS 425
           RDAFRAGA HLKRGPEFSLFDTCYDLSGQS+VKVPTVVLHFRGADM LPATNYLIPVD++
Sbjct: 368 RDAFRAGARHLKRGPEFSLFDTCYDLSGQSSVKVPTVVLHFRGADMPLPATNYLIPVDEN 427

Query: 426 GSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           GSFCFAFAGT+SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 428 GSFCFAFAGTISGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 454

BLAST of Lcy08g007760 vs. ExPASy TrEMBL
Match: A0A0A0K4G2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G329350 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.1e-226
Identity = 405/464 (87.28%), Postives = 424/464 (91.38%), Query Frame = 0

Query: 6   KNILLFFLLLSFSAASEFQTLILRPLPNPSPLS-FPQSQPQFDSEALDST--AALTLDLH 65
           K +LLFF +   +AASEFQTL LR LP PSPL  FP      DS++L S+  A LTLDLH
Sbjct: 8   KYLLLFFFI--STAASEFQTLTLRSLPTPSPLPLFP------DSQSLQSSPDAPLTLDLH 67

Query: 66  HLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLAQGSGE 125
           HLDSLSLNKTPTDLFNLRLHRD LRV +L + A          AGFSSSV+SGL+QGSGE
Sbjct: 68  HLDSLSLNKTPTDLFNLRLHRDTLRVHALNSRA----------AGFSSSVVSGLSQGSGE 127

Query: 126 YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIPCSSPL 185
           YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCY+QSDPIFNP+KSKSFAGIPCSSPL
Sbjct: 128 YFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPYKSKSFAGIPCSSPL 187

Query: 186 CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFV 245
           CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH NEGLFV
Sbjct: 188 CRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHHNEGLFV 247

Query: 246 GAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARFTPLI 305
           GAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASS+PSSMVFGDAAISRLARFTPLI
Sbjct: 248 GAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLI 307

Query: 306 RNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTAL 365
           RNPKL+TFYYV LIGISVGGVRVRGVS SLFKLD AGNGGVIIDSGTSVTRLTRPAYTAL
Sbjct: 308 RNPKLDTFYYVGLIGISVGGVRVRGVSPSLFKLDSAGNGGVIIDSGTSVTRLTRPAYTAL 367

Query: 366 RDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLIPVDDS 425
           RDAFR GA HLKRGPEFSLFDTCYDLSGQS+VKVPTVVLHFRGADM+LPATNYLIPVD++
Sbjct: 368 RDAFRVGARHLKRGPEFSLFDTCYDLSGQSSVKVPTVVLHFRGADMALPATNYLIPVDEN 427

Query: 426 GSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           GSFCFAFAGT+SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 428 GSFCFAFAGTISGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 453

BLAST of Lcy08g007760 vs. NCBI nr
Match: XP_023005015.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 857.1 bits (2213), Expect = 7.3e-245
Identity = 430/469 (91.68%), Postives = 447/469 (95.31%), Query Frame = 0

Query: 1   MESPPKNILLFFLLLS--FSAASEFQTLILRPLPNPSPLSFPQSQPQFDS-EALDSTAAL 60
           MESPP+N+L FF   +   SAASEFQTL LR LP PSPLSFPQS  QFD+ E L+STAAL
Sbjct: 1   MESPPRNLLFFFFFXAAVASAASEFQTLTLRRLPIPSPLSFPQS--QFDTQETLESTAAL 60

Query: 61  TLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLA 120
           T++LHHLDSLS NKTP+DLFNLRLHRDALRV SLT+L AAR+RTPL RAGFSSSVISGLA
Sbjct: 61  TVELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLA 120

Query: 121 QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIP 180
           QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGIP
Sbjct: 121 QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIP 180

Query: 181 CSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN 240
           CSSPLCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN
Sbjct: 181 CSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN 240

Query: 241 EGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLAR 300
           EGLFVGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLAR
Sbjct: 241 EGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLAR 300

Query: 301 FTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRP 360
           FTPLI NPKLETFYYVELIG SVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTRP
Sbjct: 301 FTPLILNPKLETFYYVELIGFSVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRP 360

Query: 361 AYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLI 420
           AYTALRDAFRAGA+HLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYLI
Sbjct: 361 AYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLI 420

Query: 421 PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467

BLAST of Lcy08g007760 vs. NCBI nr
Match: XP_023514169.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 851.7 bits (2199), Expect = 3.0e-243
Identity = 427/469 (91.04%), Postives = 445/469 (94.88%), Query Frame = 0

Query: 1   MESPPKNILLFFLLLS--FSAASEFQTLILRPLPNPSPLSFPQSQPQFDS-EALDSTAAL 60
           MESPP+ +L FF   +   SAASEFQTL LR LP PSPL FP  QPQFD+ E L+STAAL
Sbjct: 1   MESPPRYLLFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFP--QPQFDTQETLESTAAL 60

Query: 61  TLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLA 120
           T++LHHLDSLS NKTP+DLFNLRLHRDALRV SLT+L AAR+RTPL RAGFSSSVISGLA
Sbjct: 61  TVELHHLDSLSPNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLA 120

Query: 121 QGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIP 180
           QGSGEYFTRLGVGTPPRY+YMVLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGIP
Sbjct: 121 QGSGEYFTRLGVGTPPRYIYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIP 180

Query: 181 CSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN 240
           CSSPLCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN
Sbjct: 181 CSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDN 240

Query: 241 EGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLAR 300
           EGLFVGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLAR
Sbjct: 241 EGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLAR 300

Query: 301 FTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRP 360
           FTPLI NPKLETFYYVELIGISVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTRP
Sbjct: 301 FTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRP 360

Query: 361 AYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLI 420
           AYTALRDAFR GA+HLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYLI
Sbjct: 361 AYTALRDAFRVGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLI 420

Query: 421 PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 PVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467

BLAST of Lcy08g007760 vs. NCBI nr
Match: KAG7025313.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 849.4 bits (2193), Expect = 1.5e-242
Identity = 426/470 (90.64%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MESPPKNILLFFLLL---SFSAASEFQTLILRPLPNPSPLSFPQSQPQFDS-EALDSTAA 60
           MESPP+ +L FF        SAASEFQTL LR LP PSPL FP  QPQFD+ E L+STAA
Sbjct: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFP--QPQFDTQETLESTAA 60

Query: 61  LTLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGL 120
           LT++LHHLDSLS NKTP+DLFNLRLHRDALRV SLT+L A R+RTPL RAGFSSSVISGL
Sbjct: 61  LTVELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGL 120

Query: 121 AQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGI 180
           AQGSGEYFTRLGVGTPPRY++MVLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGI
Sbjct: 121 AQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGI 180

Query: 181 PCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD 240
           PCSSPLCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD
Sbjct: 181 PCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD 240

Query: 241 NEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLA 300
           NEGLFVGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLA
Sbjct: 241 NEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLA 300

Query: 301 RFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTR 360
           RFTPLI NPKLETFYYVELIGISVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTR
Sbjct: 301 RFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTR 360

Query: 361 PAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYL 420
           PAYTALRDAFRAGA+HLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYL
Sbjct: 361 PAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYL 420

Query: 421 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 468

BLAST of Lcy08g007760 vs. NCBI nr
Match: XP_022959948.1 (aspartyl protease family protein 2 [Cucurbita moschata])

HSP 1 Score: 848.6 bits (2191), Expect = 2.6e-242
Identity = 427/470 (90.85%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MESPPKNILLFFLLL---SFSAASEFQTLILRPLPNPSPLSFPQSQPQFDS-EALDSTAA 60
           MESPP+ +L FF        SAASEFQTL LR LP PSPL FP  QPQFD+ E L+STAA
Sbjct: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFP--QPQFDTQETLESTAA 60

Query: 61  LTLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGL 120
           LT++LHHLDSLS NKTP+DLFNLRLHRDALRV SLT+L AAR+RTPL RAGFSSSVISGL
Sbjct: 61  LTVELHHLDSLSPNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGL 120

Query: 121 AQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGI 180
           AQGSGEYFTRLGVGTP RY+YMVLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGI
Sbjct: 121 AQGSGEYFTRLGVGTPSRYIYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGI 180

Query: 181 PCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD 240
           PCSSPLCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD
Sbjct: 181 PCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHD 240

Query: 241 NEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLA 300
           NEGLFVGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLA
Sbjct: 241 NEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLA 300

Query: 301 RFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTR 360
           RFTPLI NPKLETFYYVELIGISVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTR
Sbjct: 301 RFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTR 360

Query: 361 PAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYL 420
           PAYTALRDAFRAGA+HLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYL
Sbjct: 361 PAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYL 420

Query: 421 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 468

BLAST of Lcy08g007760 vs. NCBI nr
Match: KAG6592908.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 815.8 bits (2106), Expect = 1.9e-232
Identity = 407/448 (90.85%), Postives = 425/448 (94.87%), Query Frame = 0

Query: 28  LRPLPNPSPLSFPQS--------QPQFDS-EALDSTAALTLDLHHLDSLSLNKTPTDLFN 87
           LR +  P+P S P S        QPQFD+ E L+STAALT++LHHLDSLS NKTP+DLFN
Sbjct: 61  LRRVGVPNPYSPPSSNSLSPSLPQPQFDTQETLESTAALTVELHHLDSLSTNKTPSDLFN 120

Query: 88  LRLHRDALRVQSLTALAAARTRTPLPRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYLYM 147
           LRLHRDALRV SLT+L AAR+RTPL RAGFSSSVISGLAQGSGEYFTRLGVGTPPRY++M
Sbjct: 121 LRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYIFM 180

Query: 148 VLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCSTRRHTC 207
           VLDTGSDVVWLQCSPCRKCY+QSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGC+TRRHTC
Sbjct: 181 VLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNTRRHTC 240

Query: 208 LYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRLSFP 267
           LYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGR SFP
Sbjct: 241 LYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRFSFP 300

Query: 268 SQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAISRLARFTPLIRNPKLETFYYVELIGI 327
           SQTG+RFNHKFSYCLVDRSASS+PSSMVFGDAAISRLARFTPLI NPKLETFYYVELIGI
Sbjct: 301 SQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYVELIGI 360

Query: 328 SVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGAAHLKRGPE 387
           SVGGVRVRG+SASLFKLD AGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGA+HLKRGPE
Sbjct: 361 SVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHLKRGPE 420

Query: 388 FSLFDTCYDLSGQSAVKVPTVVLHFRGADMSLPATNYLIPVDDSGSFCFAFAGTMSGLSI 447
           FSLFDTCYDLSGQSAVKVPTVVLHFRGADM+LPATNYLIPVDDSGSFCFAFAGTMSGLSI
Sbjct: 421 FSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDDSGSFCFAFAGTMSGLSI 480

Query: 448 IGNIQQQGFRVVYDLAGSRIGFAPRGCT 467
           IGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 481 IGNIQQQGFRVVYDLAGSRIGFAPRGCT 508

BLAST of Lcy08g007760 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 677.2 bits (1746), Expect = 9.6e-195
Identity = 345/477 (72.33%), Postives = 396/477 (83.02%), Query Frame = 0

Query: 7   NILLFFLLL-SFSAASEFQTLI--LRPLPNPSPLSFPQSQPQFDSEAL-----------D 66
           ++  FFL L SFS+   FQTL      LP  SP+SF   QP  DSE+L           +
Sbjct: 11  SLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSF---QPDSDSESLLESEFESGSDSE 70

Query: 67  STAALTLDLHHLDSLSLNKTPTDLFNLRLHRDALRVQSLTALAA---ARTRTPLPR-AGF 126
           S++++TL+L H+D+LS NKTP +LF+ RL RD+ RV+S+  LAA    R  T  PR  GF
Sbjct: 71  SSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGF 130

Query: 127 SSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPF 186
           SSSV+SGL+QGSGEYFTRLGVGTP RY+YMVLDTGSD+VWLQC+PCR+CY+QSDPIF+P 
Sbjct: 131 SSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPR 190

Query: 187 KSKSFAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAK 246
           KSK++A IPCSSP CRRLDS+GC+TRR TCLYQVSYGDGSFT GDF+TETLTFR N++  
Sbjct: 191 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKG 250

Query: 247 VALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFG 306
           VALGCGHDNEGLFVGAAGLLGLG+G+LSFP QTG RFN KFSYCLVDRSASS+PSS+VFG
Sbjct: 251 VALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFG 310

Query: 307 DAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSG 366
           +AA+SR+ARFTPL+ NPKL+TFYYV L+GISVGG RV GV+ASLFKLD  GNGGVIIDSG
Sbjct: 311 NAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSG 370

Query: 367 TSVTRLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADM 426
           TSVTRL RPAY A+RDAFR GA  LKR P+FSLFDTC+DLS  + VKVPTVVLHFRGAD+
Sbjct: 371 TSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADV 430

Query: 427 SLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 466
           SLPATNYLIPVD +G FCFAFAGTM GLSIIGNIQQQGFRVVYDLA SR+GFAP GC
Sbjct: 431 SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Lcy08g007760 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 612.5 bits (1578), Expect = 2.9e-175
Identity = 319/483 (66.05%), Postives = 375/483 (77.64%), Query Frame = 0

Query: 1   MESPPKNILLF--FLLLSF--SAASEFQTLILRPLPNPSPLSFPQSQPQFDSEALDSTAA 60
           ME    N L F  F +L F  SA+S++QTL++  LP+ + LS+P+S+   D    +ST +
Sbjct: 1   MERKVLNTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTS 60

Query: 61  LTLDLHHLDSLS--LNKTPTDLFNLRLHRDALRVQSLTALAAART------RTPLPRAGF 120
           L++ L H+D+LS   + +P DLFNLRL RD+LRV+S+T+LAA  T      RTP    GF
Sbjct: 61  LSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGF 120

Query: 121 SSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPF 180
           S +VISGL+QGSGEYF RLGVGTP   +YMVLDTGSDVVWLQCSPC+ CY Q+D IF+P 
Sbjct: 121 SGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPK 180

Query: 181 KSKSFAGIPCSSPLCRRL-DSSGCSTRR-HTCLYQVSYGDGSFTTGDFATETLTFRGNKI 240
           KSK+FA +PC S LCRRL DSS C TRR  TCLYQVSYGDGSFT GDF+TETLTF G ++
Sbjct: 181 KSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARV 240

Query: 241 AKVALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDR----SASSRP 300
             V LGCGHDNEGLFVGAAGLLGLGRG LSFPSQT  R+N KFSYCLVDR    S+S  P
Sbjct: 241 DHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPP 300

Query: 301 SSMVFGDAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGG 360
           S++VFG+AA+ + + FTPL+ NPKL+TFYY++L+GISVGG RV GVS S FKLD  GNGG
Sbjct: 301 STIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGG 360

Query: 361 VIIDSGTSVTRLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLH 420
           VIIDSGTSVTRLT+PAY ALRDAFR GA  LKR P +SLFDTC+DLSG + VKVPTVV H
Sbjct: 361 VIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFH 420

Query: 421 FRGADMSLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAP 466
           F G ++SLPA+NYLIPV+  G FCFAFAGTM  LSIIGNIQQQGFRV YDL GSR+GF  
Sbjct: 421 FGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLS 480

BLAST of Lcy08g007760 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-113
Identity = 235/484 (48.55%), Postives = 300/484 (61.98%), Query Frame = 0

Query: 1   MESPPKNILLFFLLLSFSAASEFQTLILRPLPNPSPLSFPQSQPQFDSEALDSTAALTLD 60
           + +PPK  +L  +    S+  + QT IL   P  S L+  + +   D    +S++ L+L+
Sbjct: 29  LSTPPKTNVLDVV----SSLQQTQT-ILSLDPTRSSLTTTKPESLSDPVFFNSSSPLSLE 88

Query: 61  LHHLDSL--SLNKTPTDLFNLRLHRDALRVQSLTALAAARTR--------------TPLP 120
           LH  D+   S +K    L   RL RD+ RV  + A                     T   
Sbjct: 89  LHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQ 148

Query: 121 RAGFSSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPI 180
               ++ V+SG +QGSGEYF+R+GVGTP + +Y+VLDTGSDV W+QC PC  CY QSDP+
Sbjct: 149 TEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPV 208

Query: 181 FNPFKSKSFAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTF-RG 240
           FNP  S ++  + CS+P C  L++S C  R + CLYQVSYGDGSFT G+ AT+T+TF   
Sbjct: 209 FNPTSSSTYKSLTCSAPQCSLLETSAC--RSNKCLYQVSYGDGSFTVGELATDTVTFGNS 268

Query: 241 NKIAKVALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPS 300
            KI  VALGCGHDNEGLF GAAGLLGLG G LS  +Q        FSYCLVDR  S + S
Sbjct: 269 GKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSS 328

Query: 301 SMVFGDAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGV 360
           S+ F    +       PL+RN K++TFYYV L G SVGG +V  +  ++F +D +G+GGV
Sbjct: 329 SLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKV-VLPDAIFDVDASGSGGV 388

Query: 361 IIDSGTSVTRLTRPAYTALRDAFRAGAAHLKRG-PEFSLFDTCYDLSGQSAVKVPTVVLH 420
           I+D GT+VTRL   AY +LRDAF     +LK+G    SLFDTCYD S  S VKVPTV  H
Sbjct: 389 ILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFH 448

Query: 421 FRGA-DMSLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFA 466
           F G   + LPA NYLIPVDDSG+FCFAFA T S LSIIGN+QQQG R+ YDL+ + IG +
Sbjct: 449 FTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLS 500

BLAST of Lcy08g007760 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.2e-109
Identity = 216/437 (49.43%), Postives = 280/437 (64.07%), Query Frame = 0

Query: 45  QFDSEALDSTAALTLDLHHLDSL--SLNKTPTDLFNLRLHRDALRVQSLTA--------- 104
           Q + +   ++++ +L LH   S+  + +     L   RL+RD  RV+SL           
Sbjct: 55  QQEEQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNI 114

Query: 105 ----LAAARTRTPLPRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWL 164
               L    T          + +ISG  QGSGEYFTR+G+G P R +YMVLDTGSDV WL
Sbjct: 115 SKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWL 174

Query: 165 QCSPCRKCYTQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSF 224
           QC+PC  CY Q++PIF P  S S+  + C +P C  L+ S C  R  TCLY+VSYGDGS+
Sbjct: 175 QCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSEC--RNATCLYEVSYGDGSY 234

Query: 225 TTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKF 284
           T GDFATETLT     +  VA+GCGH NEGLFVGAAGLLGLG G L+ PSQ        F
Sbjct: 235 TVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLN---TTSF 294

Query: 285 SYCLVDRSASSRPSSMVFGDAAISRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVS 344
           SYCLVDR + S  S++ FG  ++S  A   PL+RN +L+TFYY+ L GISVGG  ++ + 
Sbjct: 295 SYCLVDRDSDS-ASTVDFG-TSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQ-IP 354

Query: 345 ASLFKLDPAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLS 404
            S F++D +G+GG+IIDSGT+VTRL    Y +LRD+F  G   L++    ++FDTCY+LS
Sbjct: 355 QSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLS 414

Query: 405 GQSAVKVPTVVLHFRGADM-SLPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFR 464
            ++ V+VPTV  HF G  M +LPA NY+IPVD  G+FC AFA T S L+IIGN+QQQG R
Sbjct: 415 AKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTR 474

Query: 465 VVYDLAGSRIGFAPRGC 466
           V +DLA S IGF+   C
Sbjct: 475 VTFDLANSLIGFSSNKC 483

BLAST of Lcy08g007760 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.4e-108
Identity = 214/474 (45.15%), Postives = 296/474 (62.45%), Query Frame = 0

Query: 9   LLFFLL-----LSFSAASEFQTLILRPLPNPSPLSFPQSQPQFDSEAL--DSTAALTLDL 68
           L FF L     LS S++  F    +  +  P PL+   + P F++     +S++  TL L
Sbjct: 5   LFFFFLHLHLHLSSSSSISFPDFQIIDVLQP-PLTVTATLPDFNNTHFSDESSSKYTLRL 64

Query: 69  HHLDSLS--LNKTPTDLFNLRLHRDALRVQSLTALAAARTRTPLPRA-------GFSSSV 128
            H D       +      + R+ RD  RV ++    + +    +P +        F S +
Sbjct: 65  LHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV---IPSSDSRYEVNDFGSDI 124

Query: 129 ISGLAQGSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYTQSDPIFNPFKSKS 188
           +SG+ QGSGEYF R+GVG+PPR  YMV+D+GSD+VW+QC PC+ CY QSDP+F+P KS S
Sbjct: 125 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGS 184

Query: 189 FAGIPCSSPLCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALG 248
           + G+ C S +C R+++SGC +    C Y+V YGDGS+T G  A ETLTF    +  VA+G
Sbjct: 185 YTGVSCGSSVCDRIENSGCHS--GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMG 244

Query: 249 CGHDNEGLFVGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSRPSSMVFGDAAI 308
           CGH N G+F+GAAGLLG+G G +SF  Q   +    F YCLV R   S   S+VFG  A+
Sbjct: 245 CGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREAL 304

Query: 309 SRLARFTPLIRNPKLETFYYVELIGISVGGVRVRGVSASLFKLDPAGNGGVIIDSGTSVT 368
              A + PL+RNP+  +FYYV L G+ VGGVR+  +   +F L   G+GGV++D+GT+VT
Sbjct: 305 PVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRI-PLPDGVFDLTETGDGGVVMDTGTAVT 364

Query: 369 RLTRPAYTALRDAFRAGAAHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHF-RGADMSLP 428
           RL   AY A RD F++  A+L R    S+FDTCYDLSG  +V+VPTV  +F  G  ++LP
Sbjct: 365 RLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLP 424

Query: 429 ATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 466
           A N+L+PVDDSG++CFAFA + +GLSIIGNIQQ+G +V +D A   +GF P  C
Sbjct: 425 ARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LNJ31.3e-19372.33Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LS402.6e-11248.55Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE31.9e-10745.15Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q766C36.3e-7440.98Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q8S9J63.1e-7340.51Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A6J1KW813.5e-24591.68aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111498134 P... [more]
A0A6J1H7E01.2e-24290.85aspartyl protease family protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111460848... [more]
A0A1S3CHC41.0e-22888.79aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103500464 PE=3 ... [more]
A0A5A7U8Z23.9e-22888.58Aspartyl protease family protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A0A0K4G22.1e-22687.28Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G32935... [more]
Match NameE-valueIdentityDescription
XP_023005015.17.3e-24591.68aspartyl protease family protein 2 [Cucurbita maxima][more]
XP_023514169.13.0e-24391.04aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo][more]
KAG7025313.11.5e-24290.64Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022959948.12.6e-24290.85aspartyl protease family protein 2 [Cucurbita moschata][more]
KAG6592908.11.9e-23290.85Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT1G01300.19.6e-19572.33Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.9e-17566.05Eukaryotic aspartyl protease family protein [more]
AT3G18490.11.9e-11348.55Eukaryotic aspartyl protease family protein [more]
AT1G25510.11.2e-10949.43Eukaryotic aspartyl protease family protein [more]
AT3G20015.11.4e-10845.15Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 129..149
score: 41.52
coord: 343..354
score: 41.93
coord: 437..452
score: 26.83
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 17..465
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 310..461
e-value: 5.9E-36
score: 123.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 108..286
e-value: 1.8E-53
score: 183.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 287..466
e-value: 2.8E-54
score: 185.6
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 116..465
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 123..289
e-value: 8.8E-56
score: 188.9
NoneNo IPR availablePANTHERPTHR13683:SF761ASPARTYL PROTEASE FAMILY PROTEIN 2-LIKEcoord: 17..465
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 343..354
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 123..461
score: 46.765381
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 122..465
e-value: 1.51143E-140
score: 403.19

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g007760.1Lcy08g007760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity