Lcy08g004800 (gene) Sponge gourd (P93075) v1

BLAST of Lcy08g004800 vs. ExPASy Swiss-Prot
Match: K4CF70 (Fatty acid hydroperoxide lyase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HPL PE=1 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 6.7e-170
Identity = 293/477 (61.43%), Postives = 368/477 (77.15%), Query Frame = 0

Query: 39  MPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTRMEKNRSTVF 98
           M S P S +P PVTLPVR IPG YG PL G I DRLDYFWFQ PE FF  RMEK++STVF
Sbjct: 1   MNSAPLS-TPAPVTLPVRSIPGSYGLPLVGPIADRLDYFWFQKPENFFTKRMEKHKSTVF 60

Query: 99  RTNVPPSFPFI-FADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVCA 158
           RTNVPP FPF    +P V+AVLD KSF+HLFDMEIVEK NVLVGDFMPS  +TG+MRVCA
Sbjct: 61  RTNVPPCFPFFGSVNPNVVAVLDVKSFSHLFDMEIVEKANVLVGDFMPSVVYTGDMRVCA 120

Query: 159 YLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKPSYRIPLQQ 218
           YLD SE +H+++KNF  D+L+R SK W+P L   L T++ + E++++K+   S    LQ+
Sbjct: 121 YLDTSEPKHAQIKNFSQDILKRGSKTWVPTLLKELDTMFTTFEADLSKSNTASLLPALQK 180

Query: 219 ALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEIFLHSFRYP 278
            LFNFFS T+ GAD + SPE+ANSGYI +  WLA+QL+PTV +GVLQPLEEI +HSF YP
Sbjct: 181 FLFNFFSLTILGADPSVSPEIANSGYIFLDSWLAIQLAPTVSIGVLQPLEEILVHSFAYP 240

Query: 279 FFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGGFTLFLPVL 338
           FF V G Y +L  F++ + +EV+ R  ++F L ++E IHNLLF +GFNA+GGF++FLP L
Sbjct: 241 FFLVKGNYEKLVQFVKNEAKEVLSRAQTEFQLTEQEAIHNLLFILGFNAFGGFSIFLPTL 300

Query: 339 LGRILSDKTG-LQQRIIEEVRAKSG---SGLTFESVKEMELVYSVVYETLRLDPPVPSQY 398
           LG +  +K   +Q+++ +EVR K G     L+FESVKEMELV S VYETLRL PPVPSQY
Sbjct: 301 LGNLGDEKNADMQEKLRKEVRDKVGVNPENLSFESVKEMELVQSFVYETLRLSPPVPSQY 360

Query: 399 ARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLDY 458
           ARARKDFKLSSHDS++ +KKGELLCGYQPLVM+DP+VF+EPE F  ERF  EKG ELL+Y
Sbjct: 361 ARARKDFKLSSHDSVYEIKKGELLCGYQPLVMKDPKVFDEPEKFVLERFTKEKGKELLNY 420

Query: 459 LFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQRAN 511
           LFWSNGPQTG   + NKQCA KD+V LTA + VAY+F++YD ++    S+T++++A+
Sbjct: 421 LFWSNGPQTGRPTESNKQCAAKDMVTLTASLIVAYIFQKYDSVSFSSGSLTSVKKAS 476

BLAST of Lcy08g004800 vs. ExPASy Swiss-Prot
Match: Q9ZSY9 (Linolenate hydroperoxide lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74B2 PE=1 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.5e-161
Identity = 277/491 (56.42%), Postives = 362/491 (73.73%), Query Frame = 0

Query: 28  MALRVTMTSGGMPSIPSSIS-----PPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGP 87
           M LR    +   P   +S++      PP  LP+R +PG YGWPL G + DRLDYFWFQGP
Sbjct: 1   MLLRTMAATSPRPPPSTSLTSQQPPSPPSQLPLRTMPGSYGWPLVGPLSDRLDYFWFQGP 60

Query: 88  EKFFRTRMEKNRSTVFRTNVPPSFPFI-FADPKVIAVLDCKSFAHLFDMEIVEKKNVLVG 147
           +KFFRTR EK +STVFRTN+PP+FPF    +P ++AVLD KSF+HLFDM++V+K++VL+G
Sbjct: 61  DKFFRTRAEKYKSTVFRTNIPPTFPFFGNVNPNIVAVLDVKSFSHLFDMDLVDKRDVLIG 120

Query: 148 DFMPSTSFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIES 207
           DF PS  F G + V   LD +E +H+K+K F ++ L+RSSK+W+ EL SNL+  W +IES
Sbjct: 121 DFRPSLGFYGGVCVGVNLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNLNIFWGTIES 180

Query: 208 EIAKNAKPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLG 267
           EI+KN   SY  PLQ+ +F+F   +LAG D + SP++A +G+  I  WLALQ+ PT  LG
Sbjct: 181 EISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLALQVIPTAKLG 240

Query: 268 VL-QPLEEIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLF 327
           V+ QPLEEI LH++ YP   +AG Y++LY+FI++   + ++ G  +F L ++E I NLLF
Sbjct: 241 VVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFRLTRDEAIQNLLF 300

Query: 328 TMGFNAYGGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSG--LTFESVKEMELVYSV 387
            +GFNAYGGF++FLP L+GRI  D +GLQ+RI  EVR   GSG  L F++V EMELV SV
Sbjct: 301 VLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNFKTVNEMELVKSV 360

Query: 388 VYETLRLDPPVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFK 447
           VYETLR +PPVP Q+ARARKDF++SSHD++F VKKGELLCGYQPLVMRD  VF+EPE FK
Sbjct: 361 VYETLRFNPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDANVFDEPEEFK 420

Query: 448 PERFRGEKGAELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAA 507
           P+R+ GE G+ELL+YL+WSNGPQTGT    NKQCA KD+V LTA + VA +F RYD I  
Sbjct: 421 PDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVADLFLRYDTITG 480

Query: 508 EGESITALQRA 510
           +  SI A+ +A
Sbjct: 481 DSGSIKAVVKA 491

BLAST of Lcy08g004800 vs. ExPASy Swiss-Prot
Match: B3LF83 (Probable inactive linolenate hydroperoxide lyase OS=Arabidopsis thaliana OX=3702 GN=CYP74B2 PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.0e-127
Identity = 219/383 (57.18%), Postives = 288/383 (75.20%), Query Frame = 0

Query: 130 MEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELE 189
           M++V+K++VL+GDF PS  F G +RV  YLD +E +H+K+K F ++ L+RSSK+W+ EL 
Sbjct: 1   MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELR 60

Query: 190 SNLSTLWDSIESEIAKNAKPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIW 249
           SNL+  W +IESEI+KN   SY  PLQ+ +F+F   +LAG D + SP++A +G+  I  W
Sbjct: 61  SNLNIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTW 120

Query: 250 LALQLSPTVHLGVL-QPLEEIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFG 309
           LALQ+ PT  LGV+ QPLEEI LH++ YP   +AG Y++LY+FI++   + ++ G  +FG
Sbjct: 121 LALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 180

Query: 310 LKKEEVIHNLLFTMGFNAYGGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSG--LTF 369
           L ++E I NLLF +GFNAYGGF++FLP L+GRI  D +GLQ+RI  EVR   GSG  L F
Sbjct: 181 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 240

Query: 370 ESVKEMELVYSVVYETLRLDPPVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMR 429
           ++V EMELV SVVYETLR  PPVP Q+ARARKDF++SSHD++F VKKGELLCGYQPLVMR
Sbjct: 241 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 300

Query: 430 DPRVFEEPEAFKPERFRGEKGAELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFV 489
           D  VF+EPE FKP+R+ GE G+ELL+YL+WSNGPQTGT    NKQCA KD+V LTA + V
Sbjct: 301 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 360

Query: 490 AYMFRRYDLIAAEGESITALQRA 510
           A +F RYD I  +  SI A+ +A
Sbjct: 361 ADLFLRYDTITGDSGSIKAVVKA 383

BLAST of Lcy08g004800 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 8.0e-107
Identity = 204/478 (42.68%), Postives = 294/478 (61.51%), Query Frame = 0

Query: 42  IPSSISPPP-----VTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTRMEKNRST 101
           IP   S PP      TLP+R IPG YG P  G I DRLDYF+ QG E+FF++R++K +ST
Sbjct: 54  IPGITSQPPPSSDETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKST 113

Query: 102 VFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVC 161
           V+R N+PP  PFI ++P+VI +LD KSF  LFDM  VEKK++  G +MPST  TG  R+ 
Sbjct: 114 VYRANMPPG-PFIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRIL 173

Query: 162 AYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKPSYRIPLQ 221
           +YLDPSE  H+K+K  + ++++      IPE  S+ + L + +E ++A   K ++  P +
Sbjct: 174 SYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAAFNDPAE 233

Query: 222 QALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEIFLHSFRY 281
           QA FNF S+   G     +P   ++  + I  W+   L+P + +G+ + +EE  LHS R 
Sbjct: 234 QAAFNFLSRAFFGVKPIDTPLGKDAPSL-ISKWVLFNLAPILSVGLPKEVEEATLHSVRL 293

Query: 282 PFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGGFTLFLPV 341
           P   V   Y RLY+F       V+       G+ ++E  HN+LF + FN++GGF +  P 
Sbjct: 294 PPLLVQNDYHRLYEFFTSAAGSVLDE-AEQSGISRDEACHNILFAVCFNSWGGFKILFPS 353

Query: 342 L---LGRI-LSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPPVPSQ 401
           L   +GR  L   T L Q I   +++  G  +T  ++++M L+ SVVYETLR++PPV  Q
Sbjct: 354 LMKWIGRAGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQ 413

Query: 402 YARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLD 461
           Y +A+KDF L SH++ + VK+GE+L GYQP   +DP++F+ PE F  +RF GE G +L++
Sbjct: 414 YGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGE-GVKLME 473

Query: 462 YLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAE------GESIT 505
           Y+ WSNGP+T T    NKQCAGKD VV+ A +FV  +F+RYD    E      G SIT
Sbjct: 474 YVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASIT 527

BLAST of Lcy08g004800 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 386.3 bits (991), Expect = 5.2e-106
Identity = 202/474 (42.62%), Postives = 301/474 (63.50%), Query Frame = 0

Query: 50  PVTLPVRDIPGGYGWPLFGSIGDRLDYFW-FQGPEKFFRTRMEKNRSTVFRTNVPPSFPF 109
           P + P+R+IPG YG P F  I DRL+YF+   G +++FR+RM+K +STVFR N+PP  PF
Sbjct: 3   PSSKPLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPG-PF 62

Query: 110 IFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDPSESQHSK 169
           + ++PKVI +LD KSF  LFD+  VEKK++  G +MPST  TG  RV +YLDPSE +H++
Sbjct: 63  VSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQ 122

Query: 170 VKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKPSYRIPLQQALFNFFSKTLA 229
           +KN +  +L+ SS   IP+ E+  + L++ +E+E+AKN K ++    +QA F F  +   
Sbjct: 123 LKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYF 182

Query: 230 GADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEIFLHSFRYPFFPVAGRYRRL 289
            ++  ++ ++  S    I  W+   L+PT+ LG+   L+E  LH+FR P F +   Y +L
Sbjct: 183 NSNPEET-KLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKL 242

Query: 290 YDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGGFTLFLPVLLGRILSDKTGL 349
           YD+ +     V+++     G+ K+E +HN+LF + FN +GG  +  P  L  I      L
Sbjct: 243 YDYFQSVATPVMEQ-AEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENL 302

Query: 350 QQRIIEEVRAKSGS----GLTFESVKEMELVYSVVYETLRLDPPVPSQYARARKDFKLSS 409
             ++ EE+R    S     +T E++++M L  SVVYE+LR++PPVP QY +A+ +F + S
Sbjct: 303 HTQLAEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIES 362

Query: 410 HDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLDYLFWSNGPQTGT 469
           HD+ F VKKGE+L GYQP   +DP+VF+ PE + P+RF G+ G  LL Y++WSNGP+T +
Sbjct: 363 HDATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGD-GEALLKYVWWSNGPETES 422

Query: 470 AGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAE-GES-------ITALQRAN 511
              +NKQCAGKD VVL   +FV  +FRRYD    E GES       +T L+RA+
Sbjct: 423 PTVENKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRAS 472

BLAST of Lcy08g004800 vs. ExPASy TrEMBL
Match: A0A6J1GJZ6 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455056 PE=4 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 8.0e-243
Identity = 407/485 (83.92%), Postives = 447/485 (92.16%), Query Frame = 0

Query: 26  NLMALRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKF 85
           NLM+  V M SGG+PSIPSSISPPPVTLP+++IPG +G PLFGSI DRLDYFWFQGP+KF
Sbjct: 50  NLMSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKF 109

Query: 86  FRTRMEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMP 145
           FR+R+EKNRSTVFRTNVPPSFPF  +DPKVIAVLDCKSFAHLFDME+VEKKNVLVGDFMP
Sbjct: 110 FRSRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMP 169

Query: 146 STSFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAK 205
           STSFTGNMR+CAYLDPSESQHSK+KNF ID+LRRSSKIWIPELESNLST WD IESEIA 
Sbjct: 170 STSFTGNMRMCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIAN 229

Query: 206 NAKPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQP 265
           N K +YR  LQ ALFNFFSKTLAGADTAKSPEVA S YID+IIWL LQL PT+HLG+LQP
Sbjct: 230 NQKSTYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGILQP 289

Query: 266 LEEIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFN 325
           LEEIFLHSFR PFFP+AGRYRRLYDFIEK+GEEVVQRGVS+FGLKKEE IHNL+FT+GFN
Sbjct: 290 LEEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGFN 349

Query: 326 AYGGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRL 385
           AYGGF+LFLPVL+ RIL+D+TGLQQRI++EVRAKSGSGLTFESVKEMELVYS+VYE LRL
Sbjct: 350 AYGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYEALRL 409

Query: 386 DPPVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGE 445
           DPPVP+QYARARK+FKLSSHD++F +KKGELLCGYQPLVMRDP VFEEPEAFKP+RFR E
Sbjct: 410 DPPVPTQYARARKEFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREE 469

Query: 446 KGAELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITA 505
           KGA LLDYLFWSNGPQTG + + NKQCA KDLVVLTAVVFVAY+FRRYD IA EG SITA
Sbjct: 470 KGAALLDYLFWSNGPQTGMSSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITA 529

Query: 506 LQRAN 511
           LQ+ N
Sbjct: 530 LQKPN 534

BLAST of Lcy08g004800 vs. ExPASy TrEMBL
Match: Q66UT1 (13-hydroperoxide lyase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.3e-240
Identity = 402/481 (83.58%), Postives = 445/481 (92.52%), Query Frame = 0

Query: 30  LRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTR 89
           ++VTMTSGGMPSIPSSISPPPVTLP+R+IPG YG PLFGSIGDRLDYFWFQGPEKFFR+R
Sbjct: 1   MKVTMTSGGMPSIPSSISPPPVTLPLRNIPGSYGLPLFGSIGDRLDYFWFQGPEKFFRSR 60

Query: 90  MEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 149
           MEKN+STVFRTNVPPSFPF F DP+VIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF
Sbjct: 61  MEKNQSTVFRTNVPPSFPFFFTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 120

Query: 150 TGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKP 209
           TGNMRVCAYLD SESQHSK+KNF++DVLRRSS+IWI ELESNLST+WDSIESEIAK+ K 
Sbjct: 121 TGNMRVCAYLDTSESQHSKIKNFVMDVLRRSSRIWIQELESNLSTMWDSIESEIAKDTKS 180

Query: 210 SYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEI 269
           S+R  LQ  LFNFFSKTLAGADTAKSPEVA SGYID+IIWL LQL PT+H+G+LQPLEEI
Sbjct: 181 SFRNHLQPTLFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGILQPLEEI 240

Query: 270 FLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGG 329
           FLHSFR PFFP+A RY+RLYDFI+K+GEEVV+RGVS+FGL K+E IHNL+FTMGFNAYGG
Sbjct: 241 FLHSFRLPFFPIASRYQRLYDFIQKEGEEVVERGVSEFGLTKDEAIHNLIFTMGFNAYGG 300

Query: 330 FTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPPV 389
           F+LF PVLL RIL+DKTGLQQRI+EEV+AK+GSGLTFESVKEM+L+YSVVYETLRLDPPV
Sbjct: 301 FSLFFPVLLDRILNDKTGLQQRILEEVKAKTGSGLTFESVKEMDLIYSVVYETLRLDPPV 360

Query: 390 PSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAE 449
           P+QYARARKDFKLSS+DS +++KKGELLCGYQPLVMRDP+VF +P+ F P RFRGEKGA 
Sbjct: 361 PTQYARARKDFKLSSYDSAYSIKKGELLCGYQPLVMRDPKVFNKPKTFNPGRFRGEKGAA 420

Query: 450 LLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQRA 509
           LLDYLFWSNGPQTG   + NKQCAGKDLVVLTAVVFVAY+FRRYD IA EG SITA QR 
Sbjct: 421 LLDYLFWSNGPQTGLPSEHNKQCAGKDLVVLTAVVFVAYIFRRYDWIAGEGGSITAFQRT 480

Query: 510 N 511
           N
Sbjct: 481 N 481

BLAST of Lcy08g004800 vs. ExPASy TrEMBL
Match: A0A6J1HZX6 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111468182 PE=4 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.8e-239
Identity = 403/479 (84.13%), Postives = 439/479 (91.65%), Query Frame = 0

Query: 32  VTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTRME 91
           V M S G+PSIPSSISPPPVTLP+++IPG +G PLFGSI DRLDYFWFQGP+KFFR+RME
Sbjct: 2   VAMASSGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFRSRME 61

Query: 92  KNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTG 151
           KNRSTVFRTNVPPSFPFI +DPKV AVLDCKSFAHLFDME+VEKKNVLVGDFMPSTSFTG
Sbjct: 62  KNRSTVFRTNVPPSFPFILSDPKVTAVLDCKSFAHLFDMEVVEKKNVLVGDFMPSTSFTG 121

Query: 152 NMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKPSY 211
           NMRVCAYLD SESQHSK+KNF ID+LRRSSKIWIPELESNLST WD IESEIA N K S+
Sbjct: 122 NMRVCAYLDTSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQKSSF 181

Query: 212 RIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEIFL 271
           R  LQ ALFNFFSKTL GADTAKSPEVA S +ID+IIWL LQL PT+HLG+ QPLEEIFL
Sbjct: 182 RNLLQPALFNFFSKTLVGADTAKSPEVAKSAHIDVIIWLGLQLVPTIHLGIFQPLEEIFL 241

Query: 272 HSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGGFT 331
           HSFR PFFP+AGRYRRLYDFIEK+GEEVVQRGVS+FGLKK+E IHNL+FT+GFNAYGGF+
Sbjct: 242 HSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKDEAIHNLIFTLGFNAYGGFS 301

Query: 332 LFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPPVPS 391
           LFLPVL+ RIL+D+TGLQQRI++EVRAKSGSGLTFESVKEMELVYS VYETLRLDPPVP+
Sbjct: 302 LFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSFVYETLRLDPPVPT 361

Query: 392 QYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELL 451
           QYARARKDFKLSSHD++F++KKGELLCGYQPLVMRDP VFEEPEAFKP+RFR EKGA LL
Sbjct: 362 QYARARKDFKLSSHDAVFDIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKGAALL 421

Query: 452 DYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQRAN 511
           DYLFWSNGPQTG A + NKQCA KDLVVLTAVVFVAY+FRRYD IA EG SIT LQR N
Sbjct: 422 DYLFWSNGPQTGMASEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITTLQRPN 480

BLAST of Lcy08g004800 vs. ExPASy TrEMBL
Match: A0A1S4E196 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495615 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.5e-236
Identity = 398/482 (82.57%), Postives = 441/482 (91.49%), Query Frame = 0

Query: 30  LRVTMT-SGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRT 89
           + +TMT SGGMPSIPSSISPPPV+LP+R+IPG YG+PL GSI DRLDY+WFQGP+KFFRT
Sbjct: 1   MHLTMTSSGGMPSIPSSISPPPVSLPLRNIPGSYGFPLLGSISDRLDYYWFQGPDKFFRT 60

Query: 90  RMEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTS 149
           RMEKNRSTVFRTNVPPSFPF   DP+VIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPS S
Sbjct: 61  RMEKNRSTVFRTNVPPSFPFFPTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSIS 120

Query: 150 FTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAK 209
           FTGNMRVCAYLD SESQHSK+KNFI D+LRRSS+IWI ELESNLST+WD IESEIAKN  
Sbjct: 121 FTGNMRVCAYLDTSESQHSKIKNFITDILRRSSRIWISELESNLSTMWDGIESEIAKNKT 180

Query: 210 PSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEE 269
             +R  LQ ALFNFFSKTLAGADTAKSPEVA SGYID+IIWL LQL PT+H+GVLQPLEE
Sbjct: 181 SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE 240

Query: 270 IFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYG 329
           IFLHSFR PFFP+A RY+RLYDF +K+G+EVV+RGV++FGL KEE IHNL+FTMGFNAYG
Sbjct: 241 IFLHSFRLPFFPIASRYQRLYDFFQKEGKEVVERGVTEFGLTKEEAIHNLIFTMGFNAYG 300

Query: 330 GFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPP 389
           GF+LF PVLL RIL+DKTGLQQRI+EEVR+KS SGLTFESVKEM+L+YS+VYETLRLDPP
Sbjct: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSLVYETLRLDPP 360

Query: 390 VPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGA 449
           VPSQYARARKDFKL+S+DS +N+KKGELLCGYQPLVMRDP+VF+EPEAF P+RFRGEKG 
Sbjct: 361 VPSQYARARKDFKLTSYDSAYNIKKGELLCGYQPLVMRDPKVFDEPEAFNPDRFRGEKGV 420

Query: 450 ELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQR 509
            LLDYLFWSNGPQTGT  +KNKQCAGKDLVVLTAVVFVAY+F+RYD IA EG SITA QR
Sbjct: 421 ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTAVVFVAYIFKRYDSIAGEGGSITAFQR 480

Query: 510 AN 511
           AN
Sbjct: 481 AN 482

BLAST of Lcy08g004800 vs. ExPASy TrEMBL
Match: A0A0A0K5I0 (13-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G041960 PE=4 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 1.0e-234
Identity = 393/477 (82.39%), Postives = 435/477 (91.19%), Query Frame = 0

Query: 34  MTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTRMEKN 93
           MTSGGMPSIPSSISPPPV+LP+R+IPG YG PL GSIGDRLDY+WFQGP+KFFRTRMEKN
Sbjct: 1   MTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIGDRLDYYWFQGPDKFFRTRMEKN 60

Query: 94  RSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNM 153
           RSTVFRTNVPPSFPFI ADP+V+AVLDCKSFAHLFDMEIVEK NVLVGDFMPS SFTGNM
Sbjct: 61  RSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNM 120

Query: 154 RVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKPSYRI 213
           RVCAYLD SE  HSKVKNFI D+LRRSS+IWI ELESNLST+WD IE E+AKN +  +R 
Sbjct: 121 RVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRN 180

Query: 214 PLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEIFLHS 273
            LQ ALFNFFSKTLAGADTAKSPEVA SGYID+IIWL LQL PT+H+GVLQPLEEIFLHS
Sbjct: 181 FLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHS 240

Query: 274 FRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGGFTLF 333
           FR PFFP+A RY+RLYDF +K+G EVV+RGV++FGL KEE IHNL+FTMGFNAYGGF+LF
Sbjct: 241 FRLPFFPIASRYQRLYDFFQKEGAEVVERGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLF 300

Query: 334 LPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPPVPSQY 393
            PVLL RIL+DKTGLQQRI++EVR+KS SGLTFESVKEM+L+YS+VYETLRLDPPVPSQY
Sbjct: 301 FPVLLDRILNDKTGLQQRILKEVRSKSSSGLTFESVKEMDLIYSIVYETLRLDPPVPSQY 360

Query: 394 ARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLDY 453
           ARARKDFKL+S++S +N+KKGELLCGYQPLVMRDP VF+EPEAF P+RFRGEKGA LLDY
Sbjct: 361 ARARKDFKLTSYNSTYNIKKGELLCGYQPLVMRDPEVFDEPEAFNPDRFRGEKGAALLDY 420

Query: 454 LFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQRAN 511
           LFWSNGPQTGT  +KNKQCAGKDLVVLT VVFVAY+F+RYD IA EG SITA QRAN
Sbjct: 421 LFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 477

BLAST of Lcy08g004800 vs. NCBI nr
Match: KAG6572283.1 (Fatty acid hydroperoxide lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 850.9 bits (2197), Expect = 5.7e-243
Identity = 408/485 (84.12%), Postives = 446/485 (91.96%), Query Frame = 0

Query: 26  NLMALRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKF 85
           NLM+  V M SGG+PSIPSSISPPPVTLP+++IPG +G PLFGSI DRLDYFWFQGP+KF
Sbjct: 50  NLMSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKF 109

Query: 86  FRTRMEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMP 145
           FR+R+EKNRSTVFRTNVPPSFPF  +DPKVIAVLDCKSFAHLFDME+VEKKNVLVGDFMP
Sbjct: 110 FRSRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMP 169

Query: 146 STSFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAK 205
           STSFTGNMR+CAYLDPSESQHSK+KN+ ID+LRRSSKIWIPELESNLST WD IESEIA 
Sbjct: 170 STSFTGNMRMCAYLDPSESQHSKIKNYTIDILRRSSKIWIPELESNLSTFWDGIESEIAN 229

Query: 206 NAKPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQP 265
           N K SYR  LQ ALFNFFSKTLAGADTAKSPEVA S YID+IIWL LQL PT+HLG+ QP
Sbjct: 230 NQKSSYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGIFQP 289

Query: 266 LEEIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFN 325
           LEEIFLHSFR PFFP+AGRYRRLYDFIEK+GEEVVQRGVS+FGLKKEE IHNL+FT+GFN
Sbjct: 290 LEEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGFN 349

Query: 326 AYGGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRL 385
           AYGGF+LFLPVL+ RIL+D+TGLQQRI++EVRAKSGSGLTFESVKEMELVYS+VYETLRL
Sbjct: 350 AYGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLRL 409

Query: 386 DPPVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGE 445
           DPPVP+QYARARKDFKLSSHD++F +KKGELLCGYQPLVMRDP VFEEPEAFKP+RFR E
Sbjct: 410 DPPVPTQYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREE 469

Query: 446 KGAELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITA 505
           KGA LLDYLFWSNGPQTG   + NKQCA KDLVVLTAVVFVAY+FRRYD IA EG SITA
Sbjct: 470 KGAALLDYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITA 529

Query: 506 LQRAN 511
           LQ+ N
Sbjct: 530 LQKPN 534

BLAST of Lcy08g004800 vs. NCBI nr
Match: XP_022952346.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 849.4 bits (2193), Expect = 1.7e-242
Identity = 407/485 (83.92%), Postives = 447/485 (92.16%), Query Frame = 0

Query: 26  NLMALRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKF 85
           NLM+  V M SGG+PSIPSSISPPPVTLP+++IPG +G PLFGSI DRLDYFWFQGP+KF
Sbjct: 50  NLMSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKF 109

Query: 86  FRTRMEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMP 145
           FR+R+EKNRSTVFRTNVPPSFPF  +DPKVIAVLDCKSFAHLFDME+VEKKNVLVGDFMP
Sbjct: 110 FRSRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMP 169

Query: 146 STSFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAK 205
           STSFTGNMR+CAYLDPSESQHSK+KNF ID+LRRSSKIWIPELESNLST WD IESEIA 
Sbjct: 170 STSFTGNMRMCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIAN 229

Query: 206 NAKPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQP 265
           N K +YR  LQ ALFNFFSKTLAGADTAKSPEVA S YID+IIWL LQL PT+HLG+LQP
Sbjct: 230 NQKSTYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGILQP 289

Query: 266 LEEIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFN 325
           LEEIFLHSFR PFFP+AGRYRRLYDFIEK+GEEVVQRGVS+FGLKKEE IHNL+FT+GFN
Sbjct: 290 LEEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGFN 349

Query: 326 AYGGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRL 385
           AYGGF+LFLPVL+ RIL+D+TGLQQRI++EVRAKSGSGLTFESVKEMELVYS+VYE LRL
Sbjct: 350 AYGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYEALRL 409

Query: 386 DPPVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGE 445
           DPPVP+QYARARK+FKLSSHD++F +KKGELLCGYQPLVMRDP VFEEPEAFKP+RFR E
Sbjct: 410 DPPVPTQYARARKEFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREE 469

Query: 446 KGAELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITA 505
           KGA LLDYLFWSNGPQTG + + NKQCA KDLVVLTAVVFVAY+FRRYD IA EG SITA
Sbjct: 470 KGAALLDYLFWSNGPQTGMSSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITA 529

Query: 506 LQRAN 511
           LQ+ N
Sbjct: 530 LQKPN 534

BLAST of Lcy08g004800 vs. NCBI nr
Match: XP_023554457.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 847.4 bits (2188), Expect = 6.3e-242
Identity = 408/483 (84.47%), Postives = 443/483 (91.72%), Query Frame = 0

Query: 28  MALRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFR 87
           M+  V M SGG+PSIPSSISPPPV LP+++IPG +G PLFGSI DRLDYFWFQGP+KFFR
Sbjct: 1   MSTMVAMASGGVPSIPSSISPPPVALPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFR 60

Query: 88  TRMEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPST 147
           +R+EKNRSTVFRTNVPPSFPFI +DPKVIAVLDCKSFAHLFDME+VEKKNVLVGDFMPST
Sbjct: 61  SRIEKNRSTVFRTNVPPSFPFILSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMPST 120

Query: 148 SFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNA 207
           SFTGNMRVCAYLDPSESQHSK+KNF ID+LRRSSKIWIPELESNLST WD IESEIA N 
Sbjct: 121 SFTGNMRVCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQ 180

Query: 208 KPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLE 267
           K SYR  LQ ALFNFFSKTLAGADTAKSPEVA S YID+IIWL LQL PT+HLGV QPLE
Sbjct: 181 KSSYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGVFQPLE 240

Query: 268 EIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAY 327
           EIFLHSFR PFFP+AGRYRRLYDFIEK+GEEVVQRGVS+FGLKKEE IHNL+F +GFNAY
Sbjct: 241 EIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFALGFNAY 300

Query: 328 GGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDP 387
           GGF+LFLPVL+ RIL+D+TGLQQRI++EVRAKSGSGLTFESVKEMELVYS+VYETLRLDP
Sbjct: 301 GGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLRLDP 360

Query: 388 PVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKG 447
           PVP+QYARARKDFKLSSHD++F +KKGELLCGYQPLVMRDP VFEEPEAFKP+RFR EKG
Sbjct: 361 PVPTQYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKG 420

Query: 448 AELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQ 507
           A LLDYLFWSNGPQTG   + NKQCA KDLVVLTAVVFVAY+FRRYD IA EG SITALQ
Sbjct: 421 AALLDYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITALQ 480

Query: 508 RAN 511
           + N
Sbjct: 481 KPN 483

BLAST of Lcy08g004800 vs. NCBI nr
Match: KAG7011899.1 (Fatty acid hydroperoxide lyase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 847.0 bits (2187), Expect = 8.2e-242
Identity = 406/483 (84.06%), Postives = 444/483 (91.93%), Query Frame = 0

Query: 28  MALRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFR 87
           M+  V M SGG+PSIPSSISPPPVTLP+++IPG +G PLFGSI DRLDYFWFQGP+KFFR
Sbjct: 1   MSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFR 60

Query: 88  TRMEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPST 147
           +R+EKNRSTVFRTNVPPSFPF  +DPKVIAVLDCKSFAHLFDME+VEKKNVLVGDFMPST
Sbjct: 61  SRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMPST 120

Query: 148 SFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNA 207
           SFTGNMR+CAYLDPSESQHSK+KN+ ID+LRRSSKIWIPELESNLST WD IESEIA N 
Sbjct: 121 SFTGNMRMCAYLDPSESQHSKIKNYTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQ 180

Query: 208 KPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLE 267
           K SYR  LQ ALFNFFSKTLAGADTAKSPEVA S YID+IIWL LQL PT+HLG+ QPLE
Sbjct: 181 KSSYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGIFQPLE 240

Query: 268 EIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAY 327
           EIFLHSFR PFFP+AGRYRRLYDFIEK+GEEVVQRGVS+FGLKKEE IHNL+FT+GFNAY
Sbjct: 241 EIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGFNAY 300

Query: 328 GGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDP 387
           GGF+LFLPVL+ RIL+D+TGLQQRI++EVRAKSGSGLTFESVKEMELVYS+VYETLRLDP
Sbjct: 301 GGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLRLDP 360

Query: 388 PVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKG 447
           PVP+QYARARKDFKLSSHD++F +KKGELLCGYQPLVMRDP VFEEPEAFKP+RFR EKG
Sbjct: 361 PVPTQYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKG 420

Query: 448 AELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQ 507
           A LLDYLFWSNGPQTG   + NKQCA KDLVVLTAVVFVAY+FRRYD IA EG SITALQ
Sbjct: 421 AALLDYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITALQ 480

Query: 508 RAN 511
           + N
Sbjct: 481 KPN 483

BLAST of Lcy08g004800 vs. NCBI nr
Match: AAU12570.1 (13-hydroperoxide lyase [Citrullus lanatus])

HSP 1 Score: 842.0 bits (2174), Expect = 2.6e-240
Identity = 402/481 (83.58%), Postives = 445/481 (92.52%), Query Frame = 0

Query: 30  LRVTMTSGGMPSIPSSISPPPVTLPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTR 89
           ++VTMTSGGMPSIPSSISPPPVTLP+R+IPG YG PLFGSIGDRLDYFWFQGPEKFFR+R
Sbjct: 1   MKVTMTSGGMPSIPSSISPPPVTLPLRNIPGSYGLPLFGSIGDRLDYFWFQGPEKFFRSR 60

Query: 90  MEKNRSTVFRTNVPPSFPFIFADPKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 149
           MEKN+STVFRTNVPPSFPF F DP+VIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF
Sbjct: 61  MEKNQSTVFRTNVPPSFPFFFTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 120

Query: 150 TGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKP 209
           TGNMRVCAYLD SESQHSK+KNF++DVLRRSS+IWI ELESNLST+WDSIESEIAK+ K 
Sbjct: 121 TGNMRVCAYLDTSESQHSKIKNFVMDVLRRSSRIWIQELESNLSTMWDSIESEIAKDTKS 180

Query: 210 SYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEI 269
           S+R  LQ  LFNFFSKTLAGADTAKSPEVA SGYID+IIWL LQL PT+H+G+LQPLEEI
Sbjct: 181 SFRNHLQPTLFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGILQPLEEI 240

Query: 270 FLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGG 329
           FLHSFR PFFP+A RY+RLYDFI+K+GEEVV+RGVS+FGL K+E IHNL+FTMGFNAYGG
Sbjct: 241 FLHSFRLPFFPIASRYQRLYDFIQKEGEEVVERGVSEFGLTKDEAIHNLIFTMGFNAYGG 300

Query: 330 FTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPPV 389
           F+LF PVLL RIL+DKTGLQQRI+EEV+AK+GSGLTFESVKEM+L+YSVVYETLRLDPPV
Sbjct: 301 FSLFFPVLLDRILNDKTGLQQRILEEVKAKTGSGLTFESVKEMDLIYSVVYETLRLDPPV 360

Query: 390 PSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAE 449
           P+QYARARKDFKLSS+DS +++KKGELLCGYQPLVMRDP+VF +P+ F P RFRGEKGA 
Sbjct: 361 PTQYARARKDFKLSSYDSAYSIKKGELLCGYQPLVMRDPKVFNKPKTFNPGRFRGEKGAA 420

Query: 450 LLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQRA 509
           LLDYLFWSNGPQTG   + NKQCAGKDLVVLTAVVFVAY+FRRYD IA EG SITA QR 
Sbjct: 421 LLDYLFWSNGPQTGLPSEHNKQCAGKDLVVLTAVVFVAYIFRRYDWIAGEGGSITAFQRT 480

Query: 510 N 511
           N
Sbjct: 481 N 481

BLAST of Lcy08g004800 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 455.7 bits (1171), Expect = 5.0e-128
Identity = 219/383 (57.18%), Postives = 288/383 (75.20%), Query Frame = 0

Query: 130 MEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDPSESQHSKVKNFIIDVLRRSSKIWIPELE 189
           M++V+K++VL+GDF PS  F G +RV  YLD +E +H+K+K F ++ L+RSSK+W+ EL 
Sbjct: 1   MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELR 60

Query: 190 SNLSTLWDSIESEIAKNAKPSYRIPLQQALFNFFSKTLAGADTAKSPEVANSGYIDIIIW 249
           SNL+  W +IESEI+KN   SY  PLQ+ +F+F   +LAG D + SP++A +G+  I  W
Sbjct: 61  SNLNIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTW 120

Query: 250 LALQLSPTVHLGVL-QPLEEIFLHSFRYPFFPVAGRYRRLYDFIEKQGEEVVQRGVSDFG 309
           LALQ+ PT  LGV+ QPLEEI LH++ YP   +AG Y++LY+FI++   + ++ G  +FG
Sbjct: 121 LALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 180

Query: 310 LKKEEVIHNLLFTMGFNAYGGFTLFLPVLLGRILSDKTGLQQRIIEEVRAKSGSG--LTF 369
           L ++E I NLLF +GFNAYGGF++FLP L+GRI  D +GLQ+RI  EVR   GSG  L F
Sbjct: 181 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 240

Query: 370 ESVKEMELVYSVVYETLRLDPPVPSQYARARKDFKLSSHDSMFNVKKGELLCGYQPLVMR 429
           ++V EMELV SVVYETLR  PPVP Q+ARARKDF++SSHD++F VKKGELLCGYQPLVMR
Sbjct: 241 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 300

Query: 430 DPRVFEEPEAFKPERFRGEKGAELLDYLFWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFV 489
           D  VF+EPE FKP+R+ GE G+ELL+YL+WSNGPQTGT    NKQCA KD+V LTA + V
Sbjct: 301 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 360

Query: 490 AYMFRRYDLIAAEGESITALQRA 510
           A +F RYD I  +  SI A+ +A
Sbjct: 361 ADLFLRYDTITGDSGSIKAVVKA 383

BLAST of Lcy08g004800 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 382.9 bits (982), Expect = 4.1e-106
Identity = 197/446 (44.17%), Postives = 282/446 (63.23%), Query Frame = 0

Query: 53  LPVRDIPGGYGWPLFGSIGDRLDYFWFQGPEKFFRTRMEKNRSTVFRTNVPPSFPFIFAD 112
           LP+R+IPG YG P+ G I DR DYF+ QG E+FF++R+ K  STV+R N+PP   FI  +
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPG-AFIAEN 111

Query: 113 PKVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDPSESQHSKVKNF 172
           P+V+A+LD KSF  LFD++ VEKK++  G +MPST  TG  R+ +YLDPSE +H K+KN 
Sbjct: 112 PQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNL 171

Query: 173 IIDVLRRSSKIWIPELESNLSTLWDSIESEIAKNAKPSYRIPLQQALFNFFSKTLAGADT 232
           +  +L+ S     PE ++  S L+DS+E E++   K  +        FNF ++   G + 
Sbjct: 172 LFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNP 231

Query: 233 AKSPEVANSGYIDIIIWLALQLSPTVHLGVLQPLEEIFLHSFRYPFFPVAGRYRRLYD-F 292
           A +   A++  + I  W+   L P + +G+ + +EE  +H+F  P   V   Y+RLY+ F
Sbjct: 232 ADTKLKADAPGL-ITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFF 291

Query: 293 IEKQGEEVVQRGVSDFGLKKEEVIHNLLFTMGFNAYGGFTLFLPVLLGRILSDKTGLQQR 352
           +E  GE +V+      G+ +EE  HNLLF   FN +GG  +  P ++ RI      +  R
Sbjct: 292 LESAGEILVE--ADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNR 351

Query: 353 IIEEVRA---KSGSGLTFESVKEMELVYSVVYETLRLDPPVPSQYARARKDFKLSSHDSM 412
           + EE+R+    +G  LT  ++++MEL  SVVYE LR +PPV +QY RA+KD  + SHD+ 
Sbjct: 352 LAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAA 411

Query: 413 FNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLDYLFWSNGPQTGTAGDK 472
           F VK GE+L GYQPL  RDP++F+  + F PERF GE+G +LL ++ WSNGP+T T    
Sbjct: 412 FKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVG 471

Query: 473 NKQCAGKDLVVLTAVVFVAYMFRRYD 495
           NKQCAGKD VVL A +FV  +FRRYD
Sbjct: 472 NKQCAGKDFVVLVARLFVIEIFRRYD 493

BLAST of Lcy08g004800 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 61.2 bits (147), Expect = 2.7e-09
Identity = 34/143 (23.78%), Postives = 65/143 (45.45%), Query Frame = 0

Query: 358 AKSGSGLTFESVKEMELVYSVVYETLRLDPPVPSQYARARKDFKLSSHD-SMFNVKKGEL 417
           AK G  +  + + EM+++Y  + E LRL PP+      +  DF +++ D   +++ KG +
Sbjct: 323 AKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHI 382

Query: 418 LCGYQPLVMRDPRVFEEPEAFKPERF----RGEKGAELLDYLFWSNGPQTGTAGDKNKQC 477
           +        R P +F++P+ + PERF      +K A    Y+ +  G            C
Sbjct: 383 VATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGG---------RHGC 442

Query: 478 AGKDLVVLTAVVFVAYMFRRYDL 496
            G+    L      +++ R ++L
Sbjct: 443 LGEPFAYLQIKAIWSHLLRNFEL 456

BLAST of Lcy08g004800 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 59.7 bits (143), Expect = 7.9e-09
Identity = 42/159 (26.42%), Postives = 72/159 (45.28%), Query Frame = 0

Query: 335 PVLLGRILSDKTGLQQRIIEEVRAKSGSGLTFESVKEMELVYSVVYETLRLDPPVPSQYA 394
           P +  R+L +    Q+ I++E + K   GL +E +++M   ++V  E +R+ PP+   + 
Sbjct: 307 PHVYQRVLQE----QKEILKEKKEK--EGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFR 366

Query: 395 RARKDFKLSSHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLDYL 454
            A   F        F + KG  L         +P  F EPE F+P RF G  G +   Y+
Sbjct: 367 EAIDHFSFKG----FYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYTYV 426

Query: 455 FWSNGPQTGTAGDKNKQCAGKDLVVLTAVVFVAYMFRRY 494
            +  GP         + C GK+   L  ++F+  +  R+
Sbjct: 427 PFGGGP---------RMCPGKEYARLEILIFMHNLVNRF 445

BLAST of Lcy08g004800 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 57.0 bits (136), Expect = 5.1e-08
Identity = 47/165 (28.48%), Postives = 77/165 (46.67%), Query Frame = 0

Query: 348 LQQRIIEEVR---AKSGSGLTFESVKEMELVYSVVYETLRLDPPVPSQYARA-RKDFKLS 407
           +Q+R+ EE+     +    +  +  ++M  + +VV E LR  PP  +    +  +D  L 
Sbjct: 333 IQERLYEEITNVVGEEAKVVEEKDTQKMPYLKAVVMEALRRHPPGNTVLPHSVTEDTVLG 392

Query: 408 SHDSMFNVKKGELLCGYQPLVMRDPRVFEEPEAFKPERFRGEKGAELLDYLFWSNGPQTG 467
                + V K   +      + RDP+V+EEP AFKPERF GE+  E +D +  S G +  
Sbjct: 393 G----YKVPKKGTINFLVAEIGRDPKVWEEPMAFKPERFMGEE--EAVD-ITGSRGIKMM 452

Query: 468 TAGDKNKQCAGKDLVVLTAVVFVAYMFRRYDLIAAEGESITALQR 509
             G   + C G  L +L    +VA M R +     EG  +   ++
Sbjct: 453 PFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVEGHEVDLTEK 490