Lcy08g004670 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy08g004670
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	cysteine protease XCP1-like
Location	Chr08: 6157898 .. 6158558 (+)
RNA-Seq Expression	Lcy08g004670
Synteny	Lcy08g004670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCGTCGTTACTCCGTTGGGTGTGCTCGTCATCTCGCAGATCTGTATCGTATTCCTATCGTTATCATCTTAAATCTAATATTATCGTTACTCCGTTACCTGCCTTGATCCCGAATCTTCTCCGTCGCGCCTTCTCAGAGCCAACCAGGGCGCCGTCCAAGGTACTTCAATTTCTTCAACATTTTGATGGTTGATCTAATTTAATTTGTGTACATCTAATTCATGATATAAGAAAAGGAATCAGAATTAAGTAATTAATATACATACATATGTATATATTTGTAGTCGAGTGGTGGAGAAGTTGGTAAGTTATCAATGGAGGAAGCAGAAGGTTCGGAGAATTGGGAGTTGTTCAAGTCATGGATGTTGGAGAACGATAGGAGTTACGAGAGCGAGGAAGAGATGTTGAATAGGTTTCAGATATTCTGTAACAGGGTGAAGATGGTTGATGAGTCGAACAAGAAGTATCGTGGCCGTCCGACATTTGGGTTGACTTGCTTTGCAGACATGAACCGTGATGAGGTCCCGTGCCAGCGTCACCTCCATTTGGTACATCCCCGCCGGATAAGTGTGGAGGACGAGGACGAGGACGAGATCGAGATCGAGGTCGAGGACGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGACGAGGACTAG

mRNA sequence

ATGGCGGCGTCGTTACTCCGTTGGGTGTGCTCGTCATCTCGCAGATCTGTATCGTATTCCTATCGTTATCATCTTAAATCTAATATTATCGTTACTCCGTTACCTGCCTTGATCCCGAATCTTCTCCGTCGCGCCTTCTCAGAGCCAACCAGGGCGCCGTCCAAGTCGAGTGGTGGAGAAGTTGGTAAGTTATCAATGGAGGAAGCAGAAGGTTCGGAGAATTGGGAGTTGTTCAAGTCATGGATGTTGGAGAACGATAGGAGTTACGAGAGCGAGGAAGAGATGTTGAATAGGTTTCAGATATTCTGTAACAGGGTGAAGATGGTTGATGAGTCGAACAAGAAGTATCGTGGCCGTCCGACATTTGGGTTGACTTGCTTTGCAGACATGAACCGTGATGAGGTCCCGTGCCAGCGTCACCTCCATTTGGTACATCCCCGCCGGATAAGTGTGGAGGACGAGGACGAGGACGAGATCGAGATCGAGGTCGAGGACGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGACGAGGACTAG

Coding sequence (CDS)

ATGGCGGCGTCGTTACTCCGTTGGGTGTGCTCGTCATCTCGCAGATCTGTATCGTATTCCTATCGTTATCATCTTAAATCTAATATTATCGTTACTCCGTTACCTGCCTTGATCCCGAATCTTCTCCGTCGCGCCTTCTCAGAGCCAACCAGGGCGCCGTCCAAGTCGAGTGGTGGAGAAGTTGGTAAGTTATCAATGGAGGAAGCAGAAGGTTCGGAGAATTGGGAGTTGTTCAAGTCATGGATGTTGGAGAACGATAGGAGTTACGAGAGCGAGGAAGAGATGTTGAATAGGTTTCAGATATTCTGTAACAGGGTGAAGATGGTTGATGAGTCGAACAAGAAGTATCGTGGCCGTCCGACATTTGGGTTGACTTGCTTTGCAGACATGAACCGTGATGAGGTCCCGTGCCAGCGTCACCTCCATTTGGTACATCCCCGCCGGATAAGTGTGGAGGACGAGGACGAGGACGAGATCGAGATCGAGGTCGAGGACGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGACGAGGACTAG

Protein sequence

MAASLLRWVCSSSRRSVSYSYRYHLKSNIIVTPLPALIPNLLRRAFSEPTRAPSKSSGGEVGKLSMEEAEGSENWELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDEVPCQRHLHLVHPRRISVEDEDEDEIEIEVEDEEEEEEEEEEDED

Homology

[Close]

BLAST of Lcy08g004670 vs. ExPASy Swiss-Prot
Match: P00784 (Papain OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.3e-07
Identity = 26/59 (44.07%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           +LF+SWML++++ Y++ +E + RF+IF + +K +DE+NKK       GL  FADM+ DE
Sbjct: 46  QLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKK-NNSYWLGLNVFADMSNDE 103

[Close]

BLAST of Lcy08g004670 vs. ExPASy Swiss-Prot
Match: P05994 (Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3)

HSP 1 Score: 53.5 bits (127), Expect = 2.8e-06
Identity = 25/59 (42.37%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           +LF SWML+++++Y++ +E L RF+IF + +K +DE NK   G    GL  F+D++ DE
Sbjct: 46  QLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGY-WLGLNEFSDLSNDE 103

[Close]

BLAST of Lcy08g004670 vs. ExPASy Swiss-Prot
Match: P14080 (Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.6e-06
Identity = 26/59 (44.07%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           +LF SWML++++ YES +E + RF+IF + +  +DE+NKK       GL  FAD++ DE
Sbjct: 46  QLFDSWMLKHNKIYESIDEKIYRFEIFRDNLMYIDETNKK-NNSYWLGLNGFADLSNDE 103

[Close]

BLAST of Lcy08g004670 vs. ExPASy Swiss-Prot
Match: P10056 (Caricain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 3.6e-06
Identity = 26/59 (44.07%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           +LF SWML +++ YE+ +E L RF+IF + +  +DE+NKK       GL  FAD++ DE
Sbjct: 46  QLFNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKK-NNSYWLGLNEFADLSNDE 103

[Close]

BLAST of Lcy08g004670 vs. ExPASy Swiss-Prot
Match: O23791 (Fruit bromelain OS=Ananas comosus OX=4615 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.4e-05
Identity = 23/61 (37.70%), Postives = 33/61 (54.10%), Query Frame = 0


Query: 78  FKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDEVPC 137
           F+ WM E  R Y+ ++E + RFQIF N VK ++  N +     T G+  F DM + E   
Sbjct: 37  FEEWMAEYGRVYKDDDEKMRRFQIFKNNVKHIETFNSRNENSYTLGINQFTDMTKSEFVA 96

Query: 138 Q 139
           Q
Sbjct: 97  Q 97

[Close]

BLAST of Lcy08g004670 vs. ExPASy TrEMBL
Match: A0A5N6R8Z3 (Inhibitor_I29 domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_014132 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-05
Identity = 34/82 (41.46%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDEV 135
           ELF+SWM ++ +SYES EE L+RF IF + +K +DE+NKK       GL  FAD++ +E 
Sbjct: 46  ELFESWMSKHSKSYESVEEKLHRFDIFKDNLKHIDETNKKV-SNYWLGLNEFADLSHEEF 105

Query: 136 PCQRHLHLVHPRRISVEDEDED 158
             Q HL L+ P  +      ++
Sbjct: 106 K-QMHLGLIKPELLKKRQSPQE 125

[Close]

BLAST of Lcy08g004670 vs. ExPASy TrEMBL
Match: A0A0A0L4P3 (Inhibitor_I29 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G131910 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-05
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 66  MEEAEGSENWELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLT 125
           +E+AE S++W+ F SWM E+ + YES+EE L RF IF   +K + + NK+  G  TFGL 
Sbjct: 161 LEDAERSKDWKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNG-CTFGLN 220

Query: 126 CFADMNRDE 135
            ++D+   E
Sbjct: 221 QYSDLTNSE 228

[Close]

BLAST of Lcy08g004670 vs. ExPASy TrEMBL
Match: A0A2N9IUN2 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55876 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.3e-05
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           ELF+SWM ++ ++YES EE L+RF+IF + +K +DE+NKK       GL  FAD++ +E
Sbjct: 46  ELFESWMSKHSKTYESFEEKLHRFEIFKDNLKHIDETNKKV-SNYWLGLNEFADLSHEE 103

[Close]

BLAST of Lcy08g004670 vs. ExPASy TrEMBL
Match: A0A2N9EW73 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6967 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.3e-05
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           ELF+SWM ++ ++YES EE L+RF+IF + +K +DE+NKK       GL  FAD++ +E
Sbjct: 46  ELFESWMSKHSKTYESFEEKLHRFEIFKDNLKHIDETNKKV-SNYWLGLNEFADLSHEE 103

[Close]

BLAST of Lcy08g004670 vs. ExPASy TrEMBL
Match: A0A5A7UUL9 (Xylem cysteine proteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00130 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-04
Identity = 27/59 (45.76%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           ELF+SWM ++ ++Y S EE L+RF+IF + +K +DE+NKK       GL  FAD++ +E
Sbjct: 45  ELFESWMSKHSKTYRSVEEKLHRFEIFLDNLKHIDETNKKVSSY-WLGLNEFADLSHEE 102

[Close]

BLAST of Lcy08g004670 vs. NCBI nr
Match: XP_038887495.1 (pro-cathepsin H-like [Benincasa hispida])

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-07
Identity = 39/82 (47.56%), Postives = 52/82 (63.41%), Query Frame = 0


Query: 55  KSSGGEVGKLSMEEAEGSENWELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNK 114
           KS G E    S+ +A  SE+WE FKSWM  +++ Y SEEEML RF +F   +K +++ NK
Sbjct: 43  KSDGVE----SIYDAVLSEDWESFKSWMSMHNKKYGSEEEMLYRFGLFKKTLKRIEKLNK 102

Query: 115 KYRGRPTFGLTCFADMNRDEVP 137
            Y G  TFG   F+D+  DEVP
Sbjct: 103 NYTG-CTFGFNHFSDLTFDEVP 119

[Close]

BLAST of Lcy08g004670 vs. NCBI nr
Match: XP_038878031.1 (uncharacterized protein LOC120070224 [Benincasa hispida])

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-05
Identity = 39/115 (33.91%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 5   LLRWVCSSSRRSVSYSYRYHLKSNIIVTPLPALIPN------LLRRAFSEPTRAPSKSSG 64
           +L W+CSSS                  +PL  LI N      LL  AFS  T AP     
Sbjct: 8   VLHWLCSSS------------------SPLALLIRNRNGNRILLCGAFSASTLAPELEES 67

Query: 65  GEVGKLSMEEAEGSENWELFKSWMLEND---RSYESEEEMLNRFQIFCNRVKMVD 111
             +  +++E  +  ENWE FKSW+L+ D   +SY+SE+E+L +F++F  R++ ++
Sbjct: 68  TRLTNINLETQQAKENWESFKSWVLKYDKSYKSYKSEKELLYKFEVFSKRLRSIE 104

[Close]

BLAST of Lcy08g004670 vs. NCBI nr
Match: KAE8650303.1 (hypothetical protein Csa_010836 [Cucumis sativus])

HSP 1 Score: 59.3 bits (142), Expect = 3.9e-05
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 66  MEEAEGSENWELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLT 125
           +E+AE S++W+ F SWM E+ + YES+EE L RF IF   +K + + NK+  G  TFGL 
Sbjct: 153 LEDAERSKDWKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNG-CTFGLN 212

Query: 126 CFADMNRDE 135
            ++D+   E
Sbjct: 213 QYSDLTNSE 220

[Close]

BLAST of Lcy08g004670 vs. NCBI nr
Match: KAE8057439.1 (hypothetical protein FH972_014132 [Carpinus fangiana])

HSP 1 Score: 59.3 bits (142), Expect = 3.9e-05
Identity = 34/82 (41.46%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDEV 135
           ELF+SWM ++ +SYES EE L+RF IF + +K +DE+NKK       GL  FAD++ +E 
Sbjct: 46  ELFESWMSKHSKSYESVEEKLHRFDIFKDNLKHIDETNKKV-SNYWLGLNEFADLSHEEF 105

Query: 136 PCQRHLHLVHPRRISVEDEDED 158
             Q HL L+ P  +      ++
Sbjct: 106 K-QMHLGLIKPELLKKRQSPQE 125

[Close]

BLAST of Lcy08g004670 vs. NCBI nr
Match: XP_008451198.1 (PREDICTED: xylem cysteine proteinase 1-like [Cucumis melo] >KAA0059602.1 xylem cysteine proteinase 1-like [Cucumis melo var. makuwa] >TYK08135.1 xylem cysteine proteinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 55.8 bits (133), Expect = 4.3e-04
Identity = 27/59 (45.76%), Postives = 41/59 (69.49%), Query Frame = 0


Query: 76  ELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMNRDE 135
           ELF+SWM ++ ++Y S EE L+RF+IF + +K +DE+NKK       GL  FAD++ +E
Sbjct: 45  ELFESWMSKHSKTYRSVEEKLHRFEIFLDNLKHIDETNKKVSSY-WLGLNEFADLSHEE 102

[Close]

BLAST of Lcy08g004670 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 42.0 bits (97), Expect = 6.0e-04
Identity = 21/63 (33.33%), Postives = 35/63 (55.56%), Query Frame = 0


Query: 72  SENWELFKSWMLENDRSYESEEEMLNRFQIFCNRVKMVDESNKKYRGRPTFGLTCFADMN 131
           +E   +++ W++EN ++Y    E   RF+IF + +K V+E +         GLT FAD+ 
Sbjct: 37  AEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLT 96

Query: 132 RDE 135
            DE
Sbjct: 97  NDE 99

The following BLAST results are available for this feature:

BLAST of Lcy08g004670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P00784	7.3e-07	44.07	Papain OS=Carica papaya OX=3649 PE=1 SV=1	[more]
P05994	2.8e-06	42.37	Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3	[more]
P14080	3.6e-06	44.07	Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2	[more]
P10056	3.6e-06	44.07	Caricain OS=Carica papaya OX=3649 PE=1 SV=2	[more]
O23791	1.4e-05	37.70	Fruit bromelain OS=Ananas comosus OX=4615 PE=1 SV=1	[more]

BLAST of Lcy08g004670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5N6R8Z3	1.9e-05	41.46	Inhibitor_I29 domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_...	[more]
A0A0A0L4P3	1.9e-05	43.48	Inhibitor_I29 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G1319...	[more]
A0A2N9IUN2	9.3e-05	47.46	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55876 PE=3 SV=1	[more]
A0A2N9EW73	9.3e-05	47.46	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6967 PE=3 SV=1	[more]
A0A5A7UUL9	2.1e-04	45.76	Xylem cysteine proteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Lcy08g004670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887495.1	1.4e-07	47.56	pro-cathepsin H-like [Benincasa hispida]	[more]
XP_038878031.1	1.7e-05	33.91	uncharacterized protein LOC120070224 [Benincasa hispida]	[more]
KAE8650303.1	3.9e-05	43.48	hypothetical protein Csa_010836 [Cucumis sativus]	[more]
KAE8057439.1	3.9e-05	41.46	hypothetical protein FH972_014132 [Carpinus fangiana]	[more]
XP_008451198.1	4.3e-04	45.76	PREDICTED: xylem cysteine proteinase 1-like [Cucumis melo] >KAA0059602.1 xylem c...	[more]

BLAST of Lcy08g004670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19390.1	6.0e-04	33.33	Granulin repeat cysteine protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 149..176
None	No IPR available	GENE3D	1.10.287.2250		coord: 59..141 e-value: 1.3E-14 score: 55.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 148..178
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 153..178
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..68
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 78..135 e-value: 4.9E-14 score: 62.6
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 78..134 e-value: 1.3E-12 score: 47.9
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 73..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy08g004670.1	Lcy08g004670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0097655	serpin family protein binding