Lcy08g004010 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g004010
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionProtein kinase domain-containing protein
LocationChr08: 5314231 .. 5318104 (+)
RNA-Seq ExpressionLcy08g004010
SyntenyLcy08g004010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGTCAACAAACCAGGTTTTCGTCTTTGGCTAATTAAAGCGAGCTCCAGTTGGCGTGGCCTCCAATGGCGGAAAATGGCGACGCCAGAATTCTTCGACCCCACCATTCAAGTAATTTACGCCTCTATTTATCAATTCTCCGCTCCTTTTCGTTATCTTTGAATTCAATTTTGCACGTAGTTCGATTGCAATCAAACAACCATCATGCACGGCAGTTGACTTTGAACAACCCCACACATTTTCAGTCTGTTGTCACCTTCTTCAGTCCCCACCTCATTTCAATTTCAGCGACTCAGTCTTCCTGGTAAAATCGCCCAATCTTTGATTAAACCCCTTTCTTTCTACTCCCCTGTTTCTTTTAACTGCAGTTTGTTTCATTGGTGTTGGATTTTTATTTAGTTTGCCCTTAAATCATCTTTGATTGGATTGTTTTTGTGAAATTGATCGAGAGAGTTCTGAATTAATTGGTCAATACATGTACAGGTGGGAGTGGGGGAGAAAGGAAGTGGTTGACTGAGAAGTCTGGTTTGCTTGTATTTTACTTCTCGAGGGCTCTCCTTATCTTCTTCCTTTTTGTACTCTAACAGGAAGAGAGAAAGATAGAGCGAGAAAGATAGATTGATGGGGATTTGTTGGGGTTCTCCAGTTGATAATCATACCCCAAATACCACTGGACATCTGAGTTCAGGTAATTTCAGTAGGTCTTCTTTGATGTCAGTTCATTATCTTGAACTTTGTTGTTCTAATTTACTTCTATTTTAAAGTTTGTGCTTATTATCCGCAGGTTTTGCCCAAATAATTTTCCCCTTTTTGCGTTTGGAGTTGTTTGATGAAGTTTCTTTTAATCAGTCCTTGGAGTTGTTTTATGTTGTGTATTGTTTATCAATCTTTCCCATATGGGTATCTGTTTGTTTAATCTGTTTGGAGTTTCCTAATTTTTGAGTGTTTGATTGGTTTTTCCATTGATTTTTGATCTAAGACTAAGAGCTACATGCAGATAATGATATCTTGGTTATGACTTGGAAAATTTCCCTTATATGATATGATTCAAATTTGTGGCTGTAACAGTGGTATCCCAGACAACCAGTCAGACAACATCATCAACGGTCAGCAACATCTCCAAGTACAGTCAGTTCTCGGCCGCAAGTGGAGATGAAGCTTTTCCAAATGGCCAGATTTTGCCCTCTCCAAACTTGAGGGAGTATAGTTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGAGCTGAGGCAATGCTTGGTGAAGGAGGTTTTGGCAAGGTTTACAAAGGTTGGCTCGAGGAGAAGGTCCATGGAAAGAAGGGCAATTCGATGGTTATTGCAGTCAAAAAATTGAAATCTGACAGCTTGCAAGGCATTGAGGAGTGGCAGGTAATTATATTGTGAATCGTGATAGAGAAGAATATTCTCTGCTCTGAAATCTCCTCGAACAAATGATCGGTGGAAGGGGGAAATTTTCTATTTGGAGATTGTTCACTCTACATCCTAGGTCCGAGCTCCTAGGTTGAAAACTTGATAACTTAAAATTCTTGTTGTCCCTTAAAGACGACCATAGGATGGGTGAAGCCTCTCTAAGAATTAGTGTGTTGAACTATGAGCATAAATCAAAGAGTGAGCTATGAAACCCTGGCTATCAAAAAGAAAAAAGAAAATAAAAGAAGAAATTGGTATTAGCTCTAGAGAGAATGACTTTGGGTAGAGTATCATGTGAACTCAACTGTGAATTACTTGAGTGCAGTTGAACCCTCTAAGTGCTGCTTGAGTAAAACTCAACGAAAACAACTTAAGAAAAACACCTTAATGTGATTTCCATCATTCATGTTTGTGTCATTGATACACTGCAAGCAAACTTCTGGCAGTGGCAACTACCATAAATCAGTTTTAAATTAGGCAAGATTGAGCTAACAGAATTGCCAACTCTAGAGAGAATTGCTTGATGGGATGGGAACATATGAACTACCTCACTGTGTTTTTGTTAATATATACAGTAACGATCTCTGTCAACTTCCATAGCTCAACATTTAAACTCGGTTAAAACTAAGCCACCAGAATTGTCCACCCACGAATCGATCAATTTGTTACTGACATTTCCAATTTTGGTAGTGACAGTTGCATATCCCAAAAATTTTCTTCTCAAGACTTTTTGAAAAGTTAACTTATAATGACCTTGTCTGGTTGGTTGTGCAGTCAGAAGTGGGCTTCTTGGGAAGACTTTCTCACCCAAATCTAGTGAAGCTGTTGGGTTATTGTTGGGAGGATCAAGAGCTACTCCTTACTTATGAGTTCATGCAAAAAGGAAGCTTGGAGAACCATCTATTTGGAAGTAAGAAAGCTAATTGATGATATTACATGATGGCGTTTCTTTCACTTAATTGCTAGAGTTTTTATCTTTGTTCTCACCAACAGAAATGCCAATCTTAGTTTACTGAAGTGTAAACAACCTTTCTGTGAAATGCAGGGGGCTCAGCAGTTACACCACTAGAATGGGATACTCGGCTCAAGATCGCCATAGGCGCTGCCCGAGGGCTGGCCTTCTTGCACACATCAGACAAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTCGACGGGGTAAAGTTCTATTTATTATGAATTTCTACGTAAATATACACTTTAAGAAAGAACCTTTTACTCATCTCAATCTTTCATTAACTTTTCTCATGATTTTGTTTCCTTTTGATATCTTCTCTCTTTATAGTATTTGTCGTAAACCGAATCTGCCTTTATCTGCAGTCATATACAGCAAAGCTATCGGACTTCGGTTTGGCGAAATTGGGTCCTTCAGAAAGTAAATCTCATCTGACAACACGAGTTATGGGCACTCATGGTTATGCTGCCCCAGAGTACGTTACCACAGGTAGAATAAAAACCTTTCAAATTCAACTGTCCATAAATCAATCTTAATTTCAAAACTGGAGCCTGTTATCACCAAGAGATTTTCAAGTTGACATTGATGATGTTATTTATAACACAGGACATCTGTATGTTAAGAGTGATGTATATGGTTTTGGAGTTGTATTGATTGAGATGTTGACCGGTTTACGTGCACTTGATGAAAATCGTCCAACTGGGCAAGAACAGCTGGTGGATTGGATAAAACCATACTTATCAGAGAGGAGGAAGCTGAAAAGTGTGATGGACTTTCGCTTGGAAGGAAAATATCCGTCCAGATCGGCATTCCAAGTGGCTCAGCTAGCTCTACAATGTATCGAGCAAGAACAGAAAAACAGACCACCAATGAAAGAGGTCGTCGAGACATTGGAACAGATCGAAGCTATGAATGAGAGAACAATCGAGCCTAGAAACCGTGCTTCCCGTCTGGCATCGAATCGCAATGCTCATCAGCCTCTTCACCACAATTCTCCACTTCATGTGAAGCAATATGGAATCCAAGCTAATCAAACACCACCCAGGTTGAGATAGATTTCAGTGGTACTGATTCTCTTATCCCACTCTCATTTCTTCATTCATATGTTTCTTATAGATTTTAGAATGAAGTTTGTTTTCTCAGGTGTAACATTGTTGGCAGAGAGTACACATTTGTAATATGTAATTAACTACTTCCTAGGTTTTGGATGTTGGGAATTAGGACAGGTTCTGGTTATTGCATTACACTGGATTGTCAAGTTTGTTTGATCTTTGAAGTCCAGCTGTTCTTATTTTCTTGCTGTGAGTAAAAACTTAAAATTATCTGTAAAAGAAAGGAAGAGCAAAAATGATGAAGCCATATGGTCTTTTGTAATTAGAGAGAGAGGTGATAATTTAGCAAAAATGAGAAACAGGAAACAGGAACGTTATGAAACAAACTCTGGTTTATTTTGATTC

mRNA sequence

GAGTCAACAAACCAGGTTTTCGTCTTTGGCTAATTAAAGCGAGCTCCAGTTGGCGTGGCCTCCAATGGCGGAAAATGGCGACGCCAGAATTCTTCGACCCCACCATTCAAGTGGGAGTGGGGGAGAAAGGAAGTGGTTGACTGAGAAGTCTGGTTTGCTTGTATTTTACTTCTCGAGGGCTCTCCTTATCTTCTTCCTTTTTGTACTCTAACAGGAAGAGAGAAAGATAGAGCGAGAAAGATAGATTGATGGGGATTTGTTGGGGTTCTCCAGTTGATAATCATACCCCAAATACCACTGGACATCTGAGTTCAGTGGTATCCCAGACAACCAGTCAGACAACATCATCAACGGTCAGCAACATCTCCAAGTACAGTCAGTTCTCGGCCGCAAGTGGAGATGAAGCTTTTCCAAATGGCCAGATTTTGCCCTCTCCAAACTTGAGGGAGTATAGTTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGAGCTGAGGCAATGCTTGGTGAAGGAGGTTTTGGCAAGGTTTACAAAGGTTGGCTCGAGGAGAAGGTCCATGGAAAGAAGGGCAATTCGATGGTTATTGCAGTCAAAAAATTGAAATCTGACAGCTTGCAAGGCATTGAGGAGTGGCAGTCAGAAGTGGGCTTCTTGGGAAGACTTTCTCACCCAAATCTAGTGAAGCTGTTGGGTTATTGTTGGGAGGATCAAGAGCTACTCCTTACTTATGAGTTCATGCAAAAAGGAAGCTTGGAGAACCATCTATTTGGAAGGGGCTCAGCAGTTACACCACTAGAATGGGATACTCGGCTCAAGATCGCCATAGGCGCTGCCCGAGGGCTGGCCTTCTTGCACACATCAGACAAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTCGACGGGTCATATACAGCAAAGCTATCGGACTTCGGTTTGGCGAAATTGGGTCCTTCAGAAAGTAAATCTCATCTGACAACACGAGTTATGGGCACTCATGGTTATGCTGCCCCAGAGTACGTTACCACAGGACATCTGTATGTTAAGAGTGATGTATATGGTTTTGGAGTTGTATTGATTGAGATGTTGACCGGTTTACGTGCACTTGATGAAAATCGTCCAACTGGGCAAGAACAGCTGGTGGATTGGATAAAACCATACTTATCAGAGAGGAGGAAGCTGAAAAGTGTGATGGACTTTCGCTTGGAAGGAAAATATCCGTCCAGATCGGCATTCCAAGTGGCTCAGCTAGCTCTACAATGTATCGAGCAAGAACAGAAAAACAGACCACCAATGAAAGAGGTCGTCGAGACATTGGAACAGATCGAAGCTATGAATGAGAGAACAATCGAGCCTAGAAACCGTGCTTCCCGTCTGGCATCGAATCGCAATGCTCATCAGCCTCTTCACCACAATTCTCCACTTCATGTGAAGCAATATGGAATCCAAGCTAATCAAACACCACCCAGGTTGAGATAGATTTCAGTGGTACTGATTCTCTTATCCCACTCTCATTTCTTCATTCATATGTTTCTTATAGATTTTAGAATGAAGTTTGTTTTCTCAGGTGTAACATTGTTGGCAGAGAGTACACATTTGTAATATGTAATTAACTACTTCCTAGGTTTTGGATGTTGGGAATTAGGACAGGTTCTGGTTATTGCATTACACTGGATTGTCAAGTTTGTTTGATCTTTGAAGTCCAGCTGTTCTTATTTTCTTGCTGTGAGTAAAAACTTAAAATTATCTGTAAAAGAAAGGAAGAGCAAAAATGATGAAGCCATATGGTCTTTTGTAATTAGAGAGAGAGGTGATAATTTAGCAAAAATGAGAAACAGGAAACAGGAACGTTATGAAACAAACTCTGGTTTATTTTGATTC

Coding sequence (CDS)

ATGGGGATTTGTTGGGGTTCTCCAGTTGATAATCATACCCCAAATACCACTGGACATCTGAGTTCAGTGGTATCCCAGACAACCAGTCAGACAACATCATCAACGGTCAGCAACATCTCCAAGTACAGTCAGTTCTCGGCCGCAAGTGGAGATGAAGCTTTTCCAAATGGCCAGATTTTGCCCTCTCCAAACTTGAGGGAGTATAGTTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGAGCTGAGGCAATGCTTGGTGAAGGAGGTTTTGGCAAGGTTTACAAAGGTTGGCTCGAGGAGAAGGTCCATGGAAAGAAGGGCAATTCGATGGTTATTGCAGTCAAAAAATTGAAATCTGACAGCTTGCAAGGCATTGAGGAGTGGCAGTCAGAAGTGGGCTTCTTGGGAAGACTTTCTCACCCAAATCTAGTGAAGCTGTTGGGTTATTGTTGGGAGGATCAAGAGCTACTCCTTACTTATGAGTTCATGCAAAAAGGAAGCTTGGAGAACCATCTATTTGGAAGGGGCTCAGCAGTTACACCACTAGAATGGGATACTCGGCTCAAGATCGCCATAGGCGCTGCCCGAGGGCTGGCCTTCTTGCACACATCAGACAAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTCGACGGGTCATATACAGCAAAGCTATCGGACTTCGGTTTGGCGAAATTGGGTCCTTCAGAAAGTAAATCTCATCTGACAACACGAGTTATGGGCACTCATGGTTATGCTGCCCCAGAGTACGTTACCACAGGACATCTGTATGTTAAGAGTGATGTATATGGTTTTGGAGTTGTATTGATTGAGATGTTGACCGGTTTACGTGCACTTGATGAAAATCGTCCAACTGGGCAAGAACAGCTGGTGGATTGGATAAAACCATACTTATCAGAGAGGAGGAAGCTGAAAAGTGTGATGGACTTTCGCTTGGAAGGAAAATATCCGTCCAGATCGGCATTCCAAGTGGCTCAGCTAGCTCTACAATGTATCGAGCAAGAACAGAAAAACAGACCACCAATGAAAGAGGTCGTCGAGACATTGGAACAGATCGAAGCTATGAATGAGAGAACAATCGAGCCTAGAAACCGTGCTTCCCGTCTGGCATCGAATCGCAATGCTCATCAGCCTCTTCACCACAATTCTCCACTTCATGTGAAGCAATATGGAATCCAAGCTAATCAAACACCACCCAGGTTGAGATAG

Protein sequence

MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQILPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETLEQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR
Homology
BLAST of Lcy08g004010 vs. ExPASy Swiss-Prot
Match: Q9SII6 (Probable serine/threonine-protein kinase PIX13 OS=Arabidopsis thaliana OX=3702 GN=PIX13 PE=1 SV=2)

HSP 1 Score: 545.4 bits (1404), Expect = 5.4e-154
Identity = 279/395 (70.63%), Postives = 325/395 (82.28%), Query Frame = 0

Query: 1   MGICWGSPVDN---HTPNTTGHLSSV----VSQTTSQTTSSTVSNISKYSQFSAASGDEA 60
           MG+CWGSP D+    TP++TG++SSV     S  T+ T +S  SNIS  S FS ASG++A
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSVGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDA 60

Query: 61  FPNGQILPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVI 120
           +P+GQILP PNLR +SLAEL+A+T+NFR+E +LGEGGFGKV+KGWLE+K  GK+ N  VI
Sbjct: 61  YPDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVI 120

Query: 121 AVKKLKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHL 180
           AVKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E +ELLL YE+MQKGSLENHL
Sbjct: 121 AVKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHL 180

Query: 181 FGRGSAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFG 240
           F +GSAV PL W+ RLKIAIGAA+GLAFLH S+KQVIYRDFKASNILLDGSY AK+SDFG
Sbjct: 181 FRKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFG 240

Query: 241 LAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDEN 300
           LAKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  
Sbjct: 241 LAKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPT 300

Query: 301 RPTGQEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPM 360
           RPTGQ  L +WIKP+LSERRKL+S+MD RLEGKYP +SAF+VAQLAL+C+  E KNRP M
Sbjct: 301 RPTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSM 360

Query: 361 KEVVETLEQIEAMNERTIEPR-NRASRLASNRNAH 388
           KEVVE+LE IEA NE+ +E R  RAS     +  H
Sbjct: 361 KEVVESLELIEAANEKPLERRTTRASPSIRQQQGH 395

BLAST of Lcy08g004010 vs. ExPASy Swiss-Prot
Match: P27450 (Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=CST PE=1 SV=2)

HSP 1 Score: 414.1 bits (1063), Expect = 1.9e-114
Identity = 209/383 (54.57%), Postives = 284/383 (74.15%), Query Frame = 0

Query: 12  HTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQILPSPNLREYSLA 71
           H+  TT + S+  ++ +S T ++T S++ + SQFS  S      +G++L SPNL+ Y+  
Sbjct: 19  HSHATTNNHSN-GTEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRAEAMLGEGGFGKVYKGWLEEK--VHGKKGNSMVIAVKKLKSDSLQGIEEW 131
           +LK ATKNF+ ++MLG+GGFGKVY+GW++       + G+ M++A+K+L S+S+QG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAVTPLEWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED+ELLL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           KI IGAARGLAFLH+  ++VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQLVDWIKPYL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE LVDW++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETLEQIEAMNER 371
           S + ++K +MD  ++G+Y ++ A ++A++ L CIE + KNRP MKEVVE LE I+ +N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLN-- 378

Query: 372 TIEPRNRASRLASNRNAHQPLHH 393
            +  R+   +  +N +   P H+
Sbjct: 379 VVPNRSSTKQAVANSSRSSPHHY 395

BLAST of Lcy08g004010 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.5e-108
Identity = 203/407 (49.88%), Postives = 272/407 (66.83%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFP----N 60
           MG CWG+ + + +P               ++ SS     SKY+  S+ S     P     
Sbjct: 1   MGNCWGAKISSESP--------------CRSASSPSGGTSKYASNSSVSAASVPPTPRSE 60

Query: 61  GQILPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHG--KKGNSMVIA 120
            +IL + N++ ++  EL+ AT+NFR +++LGEGGFG V+KGW++EK     K G  MVIA
Sbjct: 61  DEILEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIA 120

Query: 121 VKKLKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLF 180
           VKKL  +  QG  EW +EV +LG+LSHP LV+L+GYC ED++ LL YEFM +GSLENHLF
Sbjct: 121 VKKLNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLF 180

Query: 181 GRGSAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGL 240
            R +   PL W+ R+KIA+GAA+GLAFLH+   +VIYRDFK SN+LLD +Y AKLSDFGL
Sbjct: 181 RRSTHFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGL 240

Query: 241 AKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENR 300
           AK GP+  KSH++TRVMGT+GYAAPEY+ TGHL  KSDVY FGVVL+EML+G RALD+NR
Sbjct: 241 AKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNR 300

Query: 301 PTGQEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMK 360
           PTG+  LV+W +PYL  +R++  ++D RL G+Y    A + A LALQCI  E KNRP M+
Sbjct: 301 PTGEHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNME 360

Query: 361 EVVETLEQIEAMNERTIEP--RNRASRLASNRNAHQPLHHNSPLHVK 400
           +VV  LEQ++   E    P  + ++S   +  N  +P     P + +
Sbjct: 361 QVVAVLEQLQDSKETGANPQLQKKSSSKNAGSNGSKPSSKGKPANAR 393

BLAST of Lcy08g004010 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 7.7e-108
Identity = 190/313 (60.70%), Postives = 240/313 (76.68%), Query Frame = 0

Query: 57  GQILPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEK--VHGKKGNSMVIA 116
           G+IL SPNL+ +S AELK+AT+NFR +++LGEGGFG V+KGW++EK     + G  +VIA
Sbjct: 45  GEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIA 104

Query: 117 VKKLKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLF 176
           VKKL  D  QG +EW +EV +LG+ SH +LVKL+GYC ED+  LL YEFM +GSLENHLF
Sbjct: 105 VKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLF 164

Query: 177 GRGSAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGL 236
            RG    PL W  RLK+A+GAA+GLAFLH+S+ +VIYRDFK SNILLD  Y AKLSDFGL
Sbjct: 165 RRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGL 224

Query: 237 AKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENR 296
           AK GP   KSH++TRVMGTHGYAAPEY+ TGHL  KSDVY FGVVL+E+L+G RA+D+NR
Sbjct: 225 AKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNR 284

Query: 297 PTGQEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMK 356
           P+G+  LV+W KPYL  +RK+  V+D RL+ +Y    A +VA L+L+C+  E K RP M 
Sbjct: 285 PSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMS 344

Query: 357 EVVETLEQIEAMN 368
           EVV  LE I+++N
Sbjct: 345 EVVSHLEHIQSLN 357

BLAST of Lcy08g004010 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 2.2e-107
Identity = 204/389 (52.44%), Postives = 270/389 (69.41%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTT-GHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQI 60
           MGIC  + +   +P  +  ++SS  + +    +SS     +  ++            G+I
Sbjct: 1   MGICLSAQIKAVSPGASPKYMSSEANDSLGSKSSSVSIRTNPRTE------------GEI 60

Query: 61  LPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEK--VHGKKGNSMVIAVKK 120
           L SPNL+ ++ AELKAAT+NFR +++LGEGGFG V+KGW++E+     K G  +VIAVKK
Sbjct: 61  LQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKK 120

Query: 121 LKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRG 180
           L  D  QG +EW +EV +LG+ SHPNLVKL+GYC ED+  LL YEFM +GSLENHLF RG
Sbjct: 121 LNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRG 180

Query: 181 SAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKL 240
           S   PL W  RLK+A+GAA+GLAFLH ++  VIYRDFK SNILLD  Y AKLSDFGLAK 
Sbjct: 181 SYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKD 240

Query: 241 GPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG 300
           GP+  KSH++TR+MGT+GYAAPEY+ TGHL  KSDVY +GVVL+E+L+G RA+D+NRP G
Sbjct: 241 GPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPG 300

Query: 301 QEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVV 360
           +++LV+W +P L+ +RKL  V+D RL+ +Y    A +VA LAL+C+  E K RP M EVV
Sbjct: 301 EQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVV 360

Query: 361 ETLEQIEAMNE---RTIEPRNRASRLASN 384
             LE I+ +NE   R I+   R  R  S+
Sbjct: 361 SHLEHIQTLNEAGGRNIDMVQRRMRRRSD 377

BLAST of Lcy08g004010 vs. ExPASy TrEMBL
Match: A0A5D3DI79 (Putative serine/threonine-protein kinase Cx32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003430 PE=3 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 2.7e-217
Identity = 385/414 (93.00%), Postives = 398/414 (96.14%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPVDNHTP+TTGHLSSV+SQTTSQTTSST+SNISK SQFSAASGDE FP+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PS NLREYSLAELKAATKNFRAEA+LGEGGFGKVYKGWLEEK  G+KGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           L+DWIKP+LSERRKLK+VMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRP MKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEP-RNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIE +NE+ IE  RNRASRLASNRNAHQPLHHNSPLH+KQYG  ANQTPPR R
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of Lcy08g004010 vs. ExPASy TrEMBL
Match: A0A1S3BHT1 (probable serine/threonine-protein kinase Cx32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490212 PE=3 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 2.7e-217
Identity = 385/414 (93.00%), Postives = 398/414 (96.14%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPVDNHTP+TTGHLSSV+SQTTSQTTSST+SNISK SQFSAASGDE FP+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PS NLREYSLAELKAATKNFRAEA+LGEGGFGKVYKGWLEEK  G+KGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           L+DWIKP+LSERRKLK+VMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRP MKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEP-RNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIE +NE+ IE  RNRASRLASNRNAHQPLHHNSPLH+KQYG  ANQTPPR R
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of Lcy08g004010 vs. ExPASy TrEMBL
Match: A0A6J1C6Y9 (probable serine/threonine-protein kinase PIX13 OS=Momordica charantia OX=3673 GN=LOC111007944 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.4e-213
Identity = 372/413 (90.07%), Postives = 395/413 (95.64%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGS V NHTPNTTG+LSSVVSQTTSQTTSSTVSNIS+ S FSAASGDEA+PNGQIL
Sbjct: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PSPNL+EYSLAELKAATKNFR EA+LGEGGFGKVYKGWLEEK +GK+GN MVIAVKKLKS
Sbjct: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWED + LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL+WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           L+DWIKPYLSERR+LK+VMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRP MKEVVETL
Sbjct: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIEA NE+  EPRNRA+R+ASNRNAHQPLHHNSPLH+K+ GI A++TPPRLR
Sbjct: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413

BLAST of Lcy08g004010 vs. ExPASy TrEMBL
Match: A0A6J1JQ74 (probable serine/threonine-protein kinase PIX13 OS=Cucurbita maxima OX=3661 GN=LOC111486471 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.6e-209
Identity = 376/413 (91.04%), Postives = 383/413 (92.74%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPV NHTPNTTGHLSSVV    SQTTSSTVSNISK S FS ASGDEAFPNGQIL
Sbjct: 1   MGICWGSPVGNHTPNTTGHLSSVV----SQTTSSTVSNISKNSNFSVASGDEAFPNGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEK HGKKGNSMVIAVKKLKS
Sbjct: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGHGKKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYT KLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTPKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHL+TRVMGT+GYAAPEY+TTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG E 
Sbjct: 241 ESKSHLSTRVMGTNGYAAPEYITTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGHEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           LVDWIKPYLSERRKLK+VMDFRLEGKYPSRSAFQVA LALQCIEQEQKNRP M EVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAHLALQCIEQEQKNRPAMNEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIEAMN+R  EPRNR+ R        QPLHH+SPLHVKQYG  ANQT PRLR
Sbjct: 361 EQIEAMNDRLTEPRNRSPR--------QPLHHSSPLHVKQYGSHANQTSPRLR 401

BLAST of Lcy08g004010 vs. ExPASy TrEMBL
Match: A0A6J1EKG2 (probable serine/threonine-protein kinase PIX13 OS=Cucurbita moschata OX=3662 GN=LOC111435287 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.6e-209
Identity = 375/413 (90.80%), Postives = 385/413 (93.22%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPV NHTPNTTGHLSSVV    SQTTSSTVSNISK S FS ASGDEAFPNGQIL
Sbjct: 1   MGICWGSPVGNHTPNTTGHLSSVV----SQTTSSTVSNISKNSNFSVASGDEAFPNGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEK HGKKGNSMVIAVKKLKS
Sbjct: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGHGKKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYT KLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTPKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHL+TRVMGT+GYAAPEY++TGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG E 
Sbjct: 241 ESKSHLSTRVMGTNGYAAPEYISTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGHEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           LVDWIKPYLSERRKLK+VMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRP M EVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPAMNEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           E+IEAMN+R  EPRNR+ R        QPLHH+SPLHVKQYG+ ANQT PRLR
Sbjct: 361 ERIEAMNDRPNEPRNRSPR--------QPLHHSSPLHVKQYGVHANQTSPRLR 401

BLAST of Lcy08g004010 vs. NCBI nr
Match: XP_038886859.1 (probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886860.1 probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886861.1 probable serine/threonine-protein kinase PIX13 [Benincasa hispida])

HSP 1 Score: 780.8 bits (2015), Expect = 5.9e-222
Identity = 392/413 (94.92%), Postives = 400/413 (96.85%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPVDNHTPNTTGHLSSVVSQTT+QTTSST+SNISK SQFSAASGDE FP+GQIL
Sbjct: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTNQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PS NLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEK  G+KG SMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGLGRKGYSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           LVDWIKPYLSERRKLK+VMDFRLEGKYPSRSA+QVAQLALQCIEQEQKNRP MKEVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAYQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIE  NE+ IEPRNRASRLASNRNAHQPLHHNSPLH+KQYGI ANQTPPRLR
Sbjct: 361 EQIETANEKPIEPRNRASRLASNRNAHQPLHHNSPLHMKQYGIHANQTPPRLR 413

BLAST of Lcy08g004010 vs. NCBI nr
Match: XP_008447841.1 (PREDICTED: probable serine/threonine-protein kinase Cx32, chloroplastic [Cucumis melo] >TYK23285.1 putative serine/threonine-protein kinase Cx32 [Cucumis melo var. makuwa])

HSP 1 Score: 764.2 bits (1972), Expect = 5.7e-217
Identity = 385/414 (93.00%), Postives = 398/414 (96.14%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPVDNHTP+TTGHLSSV+SQTTSQTTSST+SNISK SQFSAASGDE FP+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PS NLREYSLAELKAATKNFRAEA+LGEGGFGKVYKGWLEEK  G+KGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           L+DWIKP+LSERRKLK+VMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRP MKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEP-RNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIE +NE+ IE  RNRASRLASNRNAHQPLHHNSPLH+KQYG  ANQTPPR R
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of Lcy08g004010 vs. NCBI nr
Match: XP_004154222.1 (probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_011658631.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_031744213.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_031744214.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >KAE8645697.1 hypothetical protein Csa_020515 [Cucumis sativus])

HSP 1 Score: 759.2 bits (1959), Expect = 1.8e-215
Identity = 380/413 (92.01%), Postives = 394/413 (95.40%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPVDNHTP+TTGH SSV+SQTTS TTSST+SNISK SQFSAASGDE FP+GQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PS NLREYSLAELKAATKNFRAEA+LGEGGFGKVYKGWLEEK  G+KGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QG+EEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGLEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           L +WIKP+LSERRKLK+VMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRP MKEVVETL
Sbjct: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIE +NE+ IE  NRASRLASNRNAHQPLHHNSPLH+KQYG  ANQTPPR R
Sbjct: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 413

BLAST of Lcy08g004010 vs. NCBI nr
Match: XP_022136188.1 (probable serine/threonine-protein kinase PIX13 [Momordica charantia])

HSP 1 Score: 751.1 bits (1938), Expect = 5.0e-213
Identity = 372/413 (90.07%), Postives = 395/413 (95.64%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGS V NHTPNTTG+LSSVVSQTTSQTTSSTVSNIS+ S FSAASGDEA+PNGQIL
Sbjct: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PSPNL+EYSLAELKAATKNFR EA+LGEGGFGKVYKGWLEEK +GK+GN MVIAVKKLKS
Sbjct: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWED + LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL+WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE 
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           L+DWIKPYLSERR+LK+VMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRP MKEVVETL
Sbjct: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIEA NE+  EPRNRA+R+ASNRNAHQPLHHNSPLH+K+ GI A++TPPRLR
Sbjct: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413

BLAST of Lcy08g004010 vs. NCBI nr
Match: KAG6589052.1 (putative serine/threonine-protein kinase PIX13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022765.1 putative serine/threonine-protein kinase PIX13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 740.0 bits (1909), Expect = 1.1e-209
Identity = 376/413 (91.04%), Postives = 385/413 (93.22%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQIL 60
           MGICWGSPV NHTPNTTGHLSSVV    SQTTSSTVSNISK S FS ASGDEAFPNGQIL
Sbjct: 1   MGICWGSPVGNHTPNTTGHLSSVV----SQTTSSTVSNISKNSNFSVASGDEAFPNGQIL 60

Query: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIAVKKLKS 120
           PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEK HGKKGNSMVIAVKKLKS
Sbjct: 61  PSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGHGKKGNSMVIAVKKLKS 120

Query: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAV 180
           DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWED ELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYT KLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTPKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQ 300
           ESKSHL+TRVMGT+GYAAPEY++TGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG E 
Sbjct: 241 ESKSHLSTRVMGTNGYAAPEYISTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGHEN 300

Query: 301 LVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETL 360
           LVDWIKPYLSERRKLK+VMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRP M EVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPAMNEVVETL 360

Query: 361 EQIEAMNERTIEPRNRASRLASNRNAHQPLHHNSPLHVKQYGIQANQTPPRLR 414
           EQIEAMN+R  EPRNR+ R        QPLHH+SPLHVKQYG+ ANQT PRLR
Sbjct: 361 EQIEAMNDRPNEPRNRSPR--------QPLHHSSPLHVKQYGVHANQTSPRLR 401

BLAST of Lcy08g004010 vs. TAIR 10
Match: AT2G17220.1 (Protein kinase superfamily protein )

HSP 1 Score: 545.4 bits (1404), Expect = 3.9e-155
Identity = 279/395 (70.63%), Postives = 325/395 (82.28%), Query Frame = 0

Query: 1   MGICWGSPVDN---HTPNTTGHLSSV----VSQTTSQTTSSTVSNISKYSQFSAASGDEA 60
           MG+CWGSP D+    TP++TG++SSV     S  T+ T +S  SNIS  S FS ASG++A
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSVGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDA 60

Query: 61  FPNGQILPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVI 120
           +P+GQILP PNLR +SLAEL+A+T+NFR+E +LGEGGFGKV+KGWLE+K  GK+ N  VI
Sbjct: 61  YPDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVI 120

Query: 121 AVKKLKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHL 180
           AVKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E +ELLL YE+MQKGSLENHL
Sbjct: 121 AVKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHL 180

Query: 181 FGRGSAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFG 240
           F +GSAV PL W+ RLKIAIGAA+GLAFLH S+KQVIYRDFKASNILLDGSY AK+SDFG
Sbjct: 181 FRKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFG 240

Query: 241 LAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDEN 300
           LAKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  
Sbjct: 241 LAKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPT 300

Query: 301 RPTGQEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPM 360
           RPTGQ  L +WIKP+LSERRKL+S+MD RLEGKYP +SAF+VAQLAL+C+  E KNRP M
Sbjct: 301 RPTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSM 360

Query: 361 KEVVETLEQIEAMNERTIEPR-NRASRLASNRNAH 388
           KEVVE+LE IEA NE+ +E R  RAS     +  H
Sbjct: 361 KEVVESLELIEAANEKPLERRTTRASPSIRQQQGH 395

BLAST of Lcy08g004010 vs. TAIR 10
Match: AT2G17220.2 (Protein kinase superfamily protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.1e-154
Identity = 278/394 (70.56%), Postives = 324/394 (82.23%), Query Frame = 0

Query: 1   MGICWGSPVDN---HTPNTTGHLSSVV---SQTTSQTTSSTVSNISKYSQFSAASGDEAF 60
           MG+CWGSP D+    TP++TG++SS     S  T+ T +S  SNIS  S FS ASG++A+
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDAY 60

Query: 61  PNGQILPSPNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKVHGKKGNSMVIA 120
           P+GQILP PNLR +SLAEL+A+T+NFR+E +LGEGGFGKV+KGWLE+K  GK+ N  VIA
Sbjct: 61  PDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVIA 120

Query: 121 VKKLKSDSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLF 180
           VKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E +ELLL YE+MQKGSLENHLF
Sbjct: 121 VKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHLF 180

Query: 181 GRGSAVTPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGL 240
            +GSAV PL W+ RLKIAIGAA+GLAFLH S+KQVIYRDFKASNILLDGSY AK+SDFGL
Sbjct: 181 RKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFGL 240

Query: 241 AKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENR 300
           AKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  R
Sbjct: 241 AKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPTR 300

Query: 301 PTGQEQLVDWIKPYLSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMK 360
           PTGQ  L +WIKP+LSERRKL+S+MD RLEGKYP +SAF+VAQLAL+C+  E KNRP MK
Sbjct: 301 PTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSMK 360

Query: 361 EVVETLEQIEAMNERTIEPR-NRASRLASNRNAH 388
           EVVE+LE IEA NE+ +E R  RAS     +  H
Sbjct: 361 EVVESLELIEAANEKPLERRTTRASPSIRQQQGH 394

BLAST of Lcy08g004010 vs. TAIR 10
Match: AT4G35600.2 (Protein kinase superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 6.0e-116
Identity = 208/384 (54.17%), Postives = 282/384 (73.44%), Query Frame = 0

Query: 11  NHTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQILPSPNLREYSL 70
           +H   T  H +   ++ +S T ++T S++ + SQFS  S      +G++L SPNL+ Y+ 
Sbjct: 20  SHAATTNNHSNG--TEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNF 79

Query: 71  AELKAATKNFRAEAMLGEGGFGKVYKGWLEEK--VHGKKGNSMVIAVKKLKSDSLQGIEE 130
            +LK ATKNF+ ++MLG+GGFGKVY+GW++       + G+ M++A+K+L S+S+QG  E
Sbjct: 80  LDLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAE 139

Query: 131 WQSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAVTPLEWDTR 190
           W+SEV FLG LSH NLVKLLGYC ED+ELLL YEFM KGSLE+HLF R     P  WD R
Sbjct: 140 WRSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLR 199

Query: 191 LKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTT 250
           +KI IGAARGLAFLH+  ++VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TT
Sbjct: 200 IKIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTT 259

Query: 251 RVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQLVDWIKPY 310
           R+MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE LVDW++P 
Sbjct: 260 RIMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPE 319

Query: 311 LSERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETLEQIEAMNE 370
           LS + ++K +MD  ++G+Y ++ A ++A++ L CIE + KNRP MKEVVE LE I+ +N 
Sbjct: 320 LSNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLN- 379

Query: 371 RTIEPRNRASRLASNRNAHQPLHH 393
             +  R+   +  +N +   P H+
Sbjct: 380 -VVPNRSSTKQAVANSSRSSPHHY 396

BLAST of Lcy08g004010 vs. TAIR 10
Match: AT4G35600.1 (Protein kinase superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.3e-115
Identity = 209/383 (54.57%), Postives = 284/383 (74.15%), Query Frame = 0

Query: 12  HTPNTTGHLSSVVSQTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQILPSPNLREYSLA 71
           H+  TT + S+  ++ +S T ++T S++ + SQFS  S      +G++L SPNL+ Y+  
Sbjct: 19  HSHATTNNHSN-GTEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRAEAMLGEGGFGKVYKGWLEEK--VHGKKGNSMVIAVKKLKSDSLQGIEEW 131
           +LK ATKNF+ ++MLG+GGFGKVY+GW++       + G+ M++A+K+L S+S+QG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAVTPLEWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED+ELLL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           KI IGAARGLAFLH+  ++VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQLVDWIKPYL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE LVDW++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRKLKSVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETLEQIEAMNER 371
           S + ++K +MD  ++G+Y ++ A ++A++ L CIE + KNRP MKEVVE LE I+ +N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLN-- 378

Query: 372 TIEPRNRASRLASNRNAHQPLHH 393
            +  R+   +  +N +   P H+
Sbjct: 379 VVPNRSSTKQAVANSSRSSPHHY 395

BLAST of Lcy08g004010 vs. TAIR 10
Match: AT1G76360.1 (Protein kinase superfamily protein )

HSP 1 Score: 399.1 bits (1024), Expect = 4.5e-111
Identity = 199/355 (56.06%), Postives = 257/355 (72.39%), Query Frame = 0

Query: 26  QTTSQTTSSTVSNISKYSQFSAASGDEAFPNGQILPSPNLREYSLAELKAATKNFRAEAM 85
           +T  Q  +  V N           G +A P    + +PNL+ ++L ELK ATKNFR E++
Sbjct: 109 ETPPQEKTRPVDNPPSKPVEKLGLGRKAVPPSGKIVTPNLKMFTLVELKTATKNFRPESV 168

Query: 86  LGEGGFGKVYKGWLEEK--VHGKKGNSMVIAVKKLKSDSLQGIEEWQSEVGFLGRLSHPN 145
           +GEGGFG+V+KGW++EK     + G  + +AVKK   DS QG+ EWQ EV FLG+  HPN
Sbjct: 169 IGEGGFGQVFKGWVDEKTLAPSRAGVGIPVAVKKSNPDSEQGLHEWQCEVRFLGKFHHPN 228

Query: 146 LVKLLGYCWEDQELLLTYEFMQKGSLENHLFGRGSAVTPLEWDTRLKIAIGAARGLAFLH 205
           LVKLLGYCWE+ + LL YE++ KGSLENHLF +G+   P  WDTRLKIAI AA+GL FLH
Sbjct: 229 LVKLLGYCWEENQFLLVYEYLPKGSLENHLFSKGAEALP--WDTRLKIAIEAAQGLTFLH 288

Query: 206 TSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTHGYAAPEYVT 265
            S+K VIYRDFKASNILLD ++ AKLSDFGLAK GP    SH+TTRVMGT GYAAPEY+ 
Sbjct: 289 NSEKSVIYRDFKASNILLDSNFHAKLSDFGLAKNGPINGFSHVTTRVMGTQGYAAPEYMA 348

Query: 266 TGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEQLVDWIKPYLSERRKLKSVMDFRL 325
           TGHLYV+SDVYGFGVVL+E+LTGLRALD NRP+ Q+ LV+W KP L++++K++ +MD RL
Sbjct: 349 TGHLYVRSDVYGFGVVLLELLTGLRALDPNRPSAQQNLVEWAKPGLNQKKKVQKMMDPRL 408

Query: 326 EGKYPSRSAFQVAQLALQCIEQEQKNRPPMKEVVETLEQIEAMNERTIEPRNRAS 379
           E KYP  +  + A+L L+C+E + KNRPPM +V+  LE +  + ++  E R + S
Sbjct: 409 EQKYPLLAVTKTAELILRCLEADPKNRPPMDDVLRELEVVRTIRDQPQEERRKRS 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SII65.4e-15470.63Probable serine/threonine-protein kinase PIX13 OS=Arabidopsis thaliana OX=3702 G... [more]
P274501.9e-11454.57Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q65XV84.5e-10849.88Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q065487.7e-10860.70Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN... [more]
P465732.2e-10752.44Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A5D3DI792.7e-21793.00Putative serine/threonine-protein kinase Cx32 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3BHT12.7e-21793.00probable serine/threonine-protein kinase Cx32, chloroplastic OS=Cucumis melo OX=... [more]
A0A6J1C6Y92.4e-21390.07probable serine/threonine-protein kinase PIX13 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1JQ741.6e-20991.04probable serine/threonine-protein kinase PIX13 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1EKG21.6e-20990.80probable serine/threonine-protein kinase PIX13 OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
XP_038886859.15.9e-22294.92probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886860... [more]
XP_008447841.15.7e-21793.00PREDICTED: probable serine/threonine-protein kinase Cx32, chloroplastic [Cucumis... [more]
XP_004154222.11.8e-21592.01probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_011658631.1... [more]
XP_022136188.15.0e-21390.07probable serine/threonine-protein kinase PIX13 [Momordica charantia][more]
KAG6589052.11.1e-20991.04putative serine/threonine-protein kinase PIX13, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
AT2G17220.13.9e-15570.63Protein kinase superfamily protein [more]
AT2G17220.21.1e-15470.56Protein kinase superfamily protein [more]
AT4G35600.26.0e-11654.17Protein kinase superfamily protein [more]
AT4G35600.11.3e-11554.57Protein kinase superfamily protein [more]
AT1G76360.14.5e-11156.06Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 164..387
e-value: 1.4E-60
score: 206.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 64..163
e-value: 8.2E-32
score: 111.4
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 73..287
e-value: 7.0E-10
score: 36.1
NoneNo IPR availablePIRSRPIRSR000605-50PIRSR000605-50coord: 72..294
e-value: 1.9E-18
score: 64.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..413
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 381..413
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 19..376
NoneNo IPR availablePANTHERPTHR45621:SF40SERINE/THREONINE-PROTEIN KINASE PIX13-RELATEDcoord: 19..376
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 86..363
e-value: 2.15879E-93
score: 280.313
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 83..360
e-value: 2.4E-46
score: 158.2
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 86..117
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 209..221
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 80..363
score: 38.237431
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 58..360

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g004010.1Lcy08g004010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity