Lcy08g003980 (gene) Sponge gourd (P93075) v1

BLAST of Lcy08g003980 vs. ExPASy Swiss-Prot
Match: Q0WQ75 (Vacuolar protein sorting-associated protein 51 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS51 PE=1 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 8.7e-286
Identity = 529/787 (67.22%), Postives = 635/787 (80.69%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           M  E  PMDEKAKRMRDLLSSFY+PD S+S S +S +    +  + IN+TSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSIN----ASFDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNL+ LLQRHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           +QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
            DAVRFYTGAMPI K YGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGAS-HEAS 300
           LKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S+ +    S     S   + A  HE +
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDA 300

Query: 301 VREFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTD 360
           VR F+EA+RAYR IF DS+ +L KLA+ L   HF+  E +IKK++ +AD L +F I+W D
Sbjct: 301 VRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWED 360

Query: 361 VLLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSL 420
           V+L+ EVL +A L D S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L
Sbjct: 361 VVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELL 420

Query: 421 QLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFM 480
           ++ LEAS+K VLQG+ ++  DFRQLL++++G+ I  ++ I  W+Q+G QDFFR+L  +F+
Sbjct: 421 KVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFL 480

Query: 481 LLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           +LSGK +S +      E   +DK+ AGL+LVLAQ+SVFIEQ  IPR+TEEIAASFSGG  
Sbjct: 481 VLSGKTSSSND----IEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNS 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           + +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVH
Sbjct: 541 QAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQVLPQGT-RKHRRTESNGS--TTSSRSNPLREEKLNRSNTQR 660
           M+VD+FL ELE VG EVKQVLPQGT RKH+RT+SNGS  TTSSRSN L  +K+ RSN+QR
Sbjct: 601 MYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQR 660

Query: 661 ARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQI 720
           ARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQI
Sbjct: 661 ARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQI 720

Query: 721 QLDMQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARD 780
           QLD+QFL+APLKE  +DEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+++
Sbjct: 721 QLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKE 779

Query: 781 QNSMSPS 784
            N+ + S
Sbjct: 781 HNNNTVS 779

BLAST of Lcy08g003980 vs. ExPASy Swiss-Prot
Match: Q54KG3 (Vacuolar protein sorting-associated protein 51 homolog OS=Dictyostelium discoideum OX=44689 GN=vps51 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 5.6e-67
Identity = 217/804 (26.99%), Postives = 396/804 (49.25%), Query Frame = 0

Query: 11  KAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSILVQKSNLDGL 70
           ++KR+R+LL ++Y P     GS +SS N    PL  I+  SFN + Y   +V+ S L+ L
Sbjct: 147 ESKRVRNLLKTYYGPGIG-EGSDSSSMN---DPLN-IDGPSFNLNSYFDSIVKSSTLNQL 206

Query: 71  LQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSV 130
           +Q+  +M +EI+ LD D++ LVY+NY KFI+ATD IK+M  N+  ME  M  L + +  +
Sbjct: 207 IQKDNQMVSEIRTLDGDMKTLVYDNYTKFINATDIIKKMKTNVENMEEGMALLSKNMDLI 266

Query: 131 QSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGA 190
            + S+ +N++L  +R+ I++L   +   +K+QF+  LP+ L  C+  +AY  AVR+Y   
Sbjct: 267 TNCSEKINSTLSVRRDRIDQLSGLQKFFQKLQFLTALPSSLNHCLAMQAYNQAVRYYNSN 326

Query: 191 MPIFKAYGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVD 250
             I K Y    SFQ+ +   +  +  +   L E+L S S S     E+A +L  L  PV+
Sbjct: 327 SGILKQYSHIPSFQNIQNECDSIMKTMKDKLYERLSSLSTSQTDCVESAEVLMDLLEPVE 386

Query: 251 SLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASVREFAEAVRA 310
            +++K LE  +  T+ L  N E  S   +    KE N +           + E++  + +
Sbjct: 387 LVRSKYLESRKHHTITLLENLEKKSVEHITDYIKELNAN----------FLSEYSYNITS 446

Query: 311 YRVIF------ADSDR-------QLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGII 370
           Y+ +F      +DS +       QL   ++DL  K+ +  +  +       + +    II
Sbjct: 447 YKSLFINRLDGSDSKKERQQSLLQLEDFSKDLFNKYLNIAKSKLSSFKDPIEKIMALEII 506

Query: 371 WTDVLLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHT----RKKE 430
            +DV  LG  L+     +         V   +   F  L + I + + Q+++    R+ E
Sbjct: 507 NSDVSRLGSELSS---HEKITNIINSTVHDQIDFYFDNLQKTIKEHIHQLNSTLNERRDE 566

Query: 431 GVQEYSLQLALEASKKTVLQGSMDVLLDFRQL----LEDQSGLIINQRNSIVDWVQEGFQ 490
            ++ ++LQ   +A+ K ++    D++L F  L    L  ++  + + +++I   +Q   Q
Sbjct: 567 VLEGHNLQELSDATSKAIVN---DIILLFANLKPFFLPTETQFLSSYQDTIFTKIQVKLQ 626

Query: 491 DFFRALVDRFMLLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITE 550
            FF  LV+   L            ++   +  ++     +LVL+ I ++ E   I  + +
Sbjct: 627 QFFLFLVNIHFL--------EYLDIIATTSNREQFSGRFLLVLSSICLYFENKGITLVVQ 686

Query: 551 EIA--ASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRT 610
            ++   + +  G +      +F   ++C+  R  G + L+++  + SQ++  +L K   +
Sbjct: 687 LMSEFITVAKQGAKKELNVFSFNAPDLCKRVRETGLQILNVFTRLSSQKLDKILKKGLES 746

Query: 611 ---PNWVKHKEPREVHMFVDLFLQELEAVGSEVKQVLP------QGTRKHRRTESNGSTT 670
               NW+  KEPR+V    D++L+E+    +E  ++LP         + H RT S G++ 
Sbjct: 747 IPNNNWLVLKEPRDVRSVNDIYLEEILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNSV 806

Query: 671 SSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLK 730
           SS ++       +R N   + S         LF++K++    V+F   SV+   +KLSLK
Sbjct: 807 SSNNSSSNTPDGHRRNPSASSSSSSNNTNTTLFEKKVD---HVDFNTLSVLIAIIKLSLK 866

Query: 731 TLQEFVRLQTFNRSGFQQIQLDMQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIP 782
           +  E +RL+TF  +G  QIQ+D+ +L+  L ++    +  D LL E     +ERC+DP+P
Sbjct: 867 SFNESLRLKTFGTNGCHQIQIDLHYLKLSLYDLFGSNS-FDNLLQECENTVTERCVDPLP 917

BLAST of Lcy08g003980 vs. ExPASy Swiss-Prot
Match: Q155U0 (Vacuolar protein sorting-associated protein 51 homolog OS=Danio rerio OX=7955 GN=vps51 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 3.6e-58
Identity = 223/831 (26.84%), Postives = 388/831 (46.69%), Query Frame = 0

Query: 13  KRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSILVQKSNLDGLLQ 72
           +R+  +L  +Y    +  G +T  +     P + IN   F+P+ Y++ L ++ +L  L+ 
Sbjct: 16  RRVHSMLKLYYG--LNEEGKATEQAES-LDPCD-INGPHFDPEIYLNKLRKECSLTELMD 75

Query: 73  RHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQS 132
               M  +I++LD+D+Q LVYENYNKFISATDTI++M N+   ME  M+ L   + ++  
Sbjct: 76  HESCMVKQIRSLDSDMQTLVYENYNKFISATDTIRKMKNDFKKMEDEMDCLSANMAAITE 135

Query: 133 RSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMP 192
            S  ++ +L ++   I KL     LLRK+QF+++LPARL KC++ +AYA AV  +  A  
Sbjct: 136 FSARISGTLQDQHAQITKLSGVHTLLRKLQFLFELPARLNKCLELQAYAQAVSSHRRARC 195

Query: 193 IFKAYGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSL 252
           + + Y    SF+  +      +  + + L++K      S +  +E   LL QLD P + L
Sbjct: 196 VLQQYSHMPSFRGIQDDCHVIMEQLAQQLRQKFRDGGSSAKDLSECVELLLQLDEPAEEL 255

Query: 253 KAKLLEKLE-QSTLDLQ-LNAEDLSSALV---IGS-----------SKEGNVSESVYGAS 312
             K L   + +   DLQ L AE   SA+     GS           S   +VS      +
Sbjct: 256 CDKFLSHAQSRLEADLQGLEAELKDSAVTDTGAGSVQKTSPGSNPVSPSSSVSNPFLSPA 315

Query: 313 HEASVREFAE------------AVRAYRVIFAD---------------SDRQLIKLAQDL 372
               + EF +             + +Y+ +F +               ++ +L      L
Sbjct: 316 AGTDILEFIDRGCNEFVSNLCLVIASYQELFINRPQESELASKNIPEMANGKLHVFVDTL 375

Query: 373 VTKHFDATEQFIKKQICSAD---LLRVFGIIWTDVLLLGEVLNDACLP----DYSLKAAQ 432
             ++F   E+ I+++    D   L+R        +  + ++L  + +P    +  ++AA+
Sbjct: 376 AARYFSLVERRIQEEKGVGDNSLLVRALDRFHRRLQAISKLLPGSAVPSQGTEIVVRAAR 435

Query: 433 VAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASKKTVLQG--SMDVLL 492
             +KQY+    S L     D+LT V  R+       S+  A  +    ++ G  S D   
Sbjct: 436 ERIKQYL----SALQTFYHDSLTDV--RQALAAPRLSVGGASASGGGALVGGASSKDAPP 495

Query: 493 DFRQLLEDQSGLIINQRNSIV---------------------DWVQEGFQDFFRALVDRF 552
              +LL   S  I+NQ  S++                     ++  +G ++       +F
Sbjct: 496 SLPELLTSLSNFILNQLKSVLASVHLFTAKDITFSNKPYFKGEFCSQGVREGLVVSFIKF 555

Query: 553 MLLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGG 612
           +  S +   Y +S       +       L+L+L+++ +  E + I  I       F    
Sbjct: 556 ICQSSR--QYCES----AGDRGGSTPPALLLLLSRLCLDYETSTISYILTLTDEQF---- 615

Query: 613 IRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREV 672
                + P      +C   R A +K L+ Y+ ++   IS +L K   T +WV   EPR V
Sbjct: 616 -LVQHHTPVTPVTALCAEAREAAQKLLNHYVKVQGLIISQMLRKSVETRDWVNTIEPRNV 675

Query: 673 HMFVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRAR 732
              +   +++  ++  +V  +  +G RK   ++S+  T S  S+  ++ +   S T  A 
Sbjct: 676 RAVMKRVVEDTTSIDVQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRQQIRYAPSYTPSAP 735

Query: 733 SQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQ 769
               L +++ KLF ++I+IF+ VEF + SV+T  +K+SLKT  E VRL+TF R G QQIQ
Sbjct: 736 MDTNLLSNIHKLFSERIDIFSPVEFNKVSVLTGIIKISLKTFLECVRLRTFGRYGLQQIQ 795

BLAST of Lcy08g003980 vs. ExPASy Swiss-Prot
Match: Q4V9Y0 (Vacuolar protein sorting-associated protein 51 homolog OS=Xenopus tropicalis OX=8364 GN=vps51 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 6.2e-58
Identity = 203/756 (26.85%), Postives = 364/756 (48.15%), Query Frame = 0

Query: 39  RYASPLEAINTTSFNPDQYMSILVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNK 98
           R+  P + ++   FNP+ Y++ L ++S+L  L+    +M  +I++LD+++Q LVYENYNK
Sbjct: 37  RHLEPTD-VDGVHFNPELYLTKLRKESSLSQLMDVEADMVRQIRSLDSEMQTLVYENYNK 96

Query: 99  FISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL 158
           FISATDTI++M N+   ME  M+ L   +  +   S  ++++L E+ + I KL     LL
Sbjct: 97  FISATDTIRKMKNDFKKMEDEMDGLATNMAVITEFSARISSTLQERHQQITKLSGVHTLL 156

Query: 159 RKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKAYGD-SSFQDCKRASEEAIAIVL 218
           RK+QF+++LPARL KCI+  AYA AV +++ A  +   Y    SF   +   +  +A + 
Sbjct: 157 RKLQFLFELPARLKKCIELGAYAQAVSYHSKARSVLHQYQHMPSFHGIQTDCQAIMAGLA 216

Query: 219 KNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLLE----KLEQSTLDLQLNAEDL 278
             L+++    + S Q  +E   +L  L+ P   L  + L     +L     DLQ + + L
Sbjct: 217 DTLRQRFRDPASSPQDLSECVEMLLNLEEPAHLLCDEFLAHGRGRLASHLSDLQESGDIL 276

Query: 279 SSALVIGSSKEGNVSES-VYGASHEASVREFAEAVRAYRVIFADSDRQLIKLAQDLVTKH 338
                +     G +S++ +  AS+++   + A +          ++ +L    ++L T +
Sbjct: 277 E---FVDRGCGGFISDACLLAASYQSLFSKEAGSTAQM------AEAKLTSFLEELSTGY 336

Query: 339 FDATEQFIKKQICSAD---LLRVFGIIWTDVLLLGEVLNDACL----PDYSLKAAQVAVK 398
           F+  E+ ++++    D   L+R        +    +++          +  ++AAQ  + 
Sbjct: 337 FELVEKRLRQEKSLGDNSLLVRALDRFHRRLQAPSKLVPGCGFNRRGTEIVVRAAQERLA 396

Query: 399 QYVTCT---FSRLLQDISDALTQVHTRKKEGVQEYSLQLALEASK----KTVLQG----- 458
           QY+      F   L D+  AL       KE      L   L AS     KTVL       
Sbjct: 397 QYLQALKDFFQGCLTDVRQALAAPRLPGKEMPALGDLLAGLSASVLNQIKTVLAAVHLFT 456

Query: 459 SMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFMLLSGKNNSYSQSQV 518
           + DV    +   + +      +   IV +++   Q        +F  + G+  +      
Sbjct: 457 AKDVAFSDKPYFKGEFCSQGVREGLIVAFIKSVCQ-----TARQFCEIPGEKGT------ 516

Query: 519 LTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFVPAEI 578
                        L+L+L+++ +  E + I  I       F      G ++ P    + +
Sbjct: 517 --------STPPALLLLLSRLCLDYETSTISYILTLTDEQF-----LGQDHSPVTPVSSL 576

Query: 579 CRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVG 638
           C + R+  +  L+ Y+  +   +S +L K   T +WV   EPR V   +   ++++  V 
Sbjct: 577 CSLARSTAQTLLNQYVKSQGLVVSQMLRKSVETRDWVTTIEPRNVRAVMKRVVEDITGVD 636

Query: 639 SEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQL-LETHLAKLFKQ 698
            +V  +  +G RK   ++S+  T S  S+   + +  +S T  A     L +++ KLF +
Sbjct: 637 VQVGLLYEEGVRKAHSSDSSKRTFSVYSSSRLQGRYAQSYTPSAPMDTNLLSNIQKLFSE 696

Query: 699 KIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRAPLKEIAD 758
           +I+IF+ V+F + S++T  +K+SLKT  E VRL+TF R G QQIQ+D  +L+  L     
Sbjct: 697 RIDIFSTVQFNKVSILTGIIKISLKTFLECVRLRTFGRYGLQQIQVDCHYLQLYLWRFVS 756

Query: 759 DEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQ 769
           DE L+  LLDEV+ +A+ RCLDP P+E  +++ + +
Sbjct: 757 DENLVHCLLDEVVGSAAHRCLDPAPMEQSVIEVICE 758

BLAST of Lcy08g003980 vs. ExPASy Swiss-Prot
Match: Q3UVL4 (Vacuolar protein sorting-associated protein 51 homolog OS=Mus musculus OX=10090 GN=Vps51 PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 1.2e-56
Identity = 207/784 (26.40%), Postives = 366/784 (46.68%), Query Frame = 0

Query: 10  EKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEA--INTTSFNPDQYMSILVQKSNL 69
           E+ ++   +L  +Y     +S    +       PL+   +N   F+P+ Y+  L ++  L
Sbjct: 28  ERRRKAHGMLKLYY----GLSEGEVAGHPAGPDPLDPTDLNGAHFDPEVYLDKLRRECPL 87

Query: 70  DGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNMEQLLEKI 129
             L+    +M  +I+ LD+D+Q LVYENYNKFISATDTI++M N+   ME  M++L   +
Sbjct: 88  AQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATNM 147

Query: 130 LSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAYADAVRFY 189
             + + S  ++ +L ++ E I KL     LLRK+QF+++LP+RL KC++  AY  AVR+ 
Sbjct: 148 AVITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQ 207

Query: 190 TGAMPIFKAYGD-SSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVLLKQLDF 249
             A  + + Y    SF+  +   +   A + + L+++          +AE   LL  L  
Sbjct: 208 GRARAVLQQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALGE 267

Query: 250 PVDSLKAKLLE----KLEQ--STLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 309
           P + L  + L     +LE+  S+L+ +L     +  ++  + + GN             V
Sbjct: 268 PAEELCEEFLAHARGRLEEELSSLEAELGPSPPAPDVLEFTDRGGN-----------GFV 327

Query: 310 REFAEAVRAYRVIFADSD----RQLIKLAQDLVTKHFDATEQFIKKQICSAD---LLRVF 369
               +   AY+ +FA        +L   AQ+L  ++F   E+ + ++   +D   L+R  
Sbjct: 328 GGLCQVAAAYQELFAAQGPAGAEKLAAFAQELGGRYFALVERRLAQEQGGSDNSLLVRAL 387

Query: 370 GIIWTDVLLLGEVLNDACLPDYSLKAAQVAVK-------QYVTCTFSRLLQDISDALTQV 429
                 +   G +L  A L + + +  +   +       Q +   F   L D+  AL   
Sbjct: 388 DRFHRRLRAPGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLSSLTDVRQALAAP 447

Query: 430 HTRKKEGVQEYSLQLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEG 489
               KEG     L   + +S  + ++ S+  +  F       S     +       V+EG
Sbjct: 448 RLAGKEGPSLAELLANVASSILSHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVREG 507

Query: 490 -FQDFFRALVDRFMLLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPR 549
               F R++              +QS   +   +       L+L+L+++ +  E   I  
Sbjct: 508 LIVGFIRSMCQT-----------AQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISY 567

Query: 550 ITEEIAASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFR 609
           I       F        +  P    + +C   R    + L  Y+ ++   IS +L K   
Sbjct: 568 ILTLTDEQF-----LVQDQSPVTPVSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVE 627

Query: 610 TPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLR 669
           T +W+   EPR V   +   +++  A+  +V  +  +G RK + ++S+  T S  S+  +
Sbjct: 628 TRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLLYEEGVRKAQSSDSSKRTFSVYSSSRQ 687

Query: 670 EEKLNRSNTQRARSQL-LETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVR 729
           + +   S T  A     L +++ KLF ++I++F+ VEF + SV+T  +K+SLKTL E VR
Sbjct: 688 QGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVLTGIIKISLKTLLECVR 747

Query: 730 LQTFNRSGFQQIQLDMQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILD 769
           L+TF R G QQ+Q+D  FL+  L     DE L+  LLDEV+ +A+ RC DP+P+EP +++
Sbjct: 748 LRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPMEPSVVE 780

BLAST of Lcy08g003980 vs. ExPASy TrEMBL
Match: A0A6J1EMM4 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111435972 PE=3 SV=1)

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 745/783 (95.15%), Postives = 763/783 (97.45%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI++ PMDEK KRMRDLLSSFYSPDAS SGSS  SSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSK+GNVSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEA+RAYRVIFADSDRQLIKLAQDLVTKHFDATEQFI+KQIC+ADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQVAVKQYVTCTFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+SIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+S+SQSQVL EATQA+KVVAGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAVGSEVKQ+LPQGTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS 780
           MQFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN 
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 780

Query: 781 MSP 783
            SP
Sbjct: 781 TSP 783

BLAST of Lcy08g003980 vs. ExPASy TrEMBL
Match: A0A1S3CES5 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucumis melo OX=3656 GN=LOC103500085 PE=3 SV=1)

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 741/782 (94.76%), Postives = 759/782 (97.06%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMS S T SSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSK+GN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REF EAVRAYRVIFADSDRQLIKLAQDLVTKHFD+TEQFIKKQIC+ADLL VFG IWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA L DYSLKAAQVAVKQYVT TFSRLLQDISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFML 480
           L LEASKK VLQGSMD+LLDFRQLLEDQSGLIINQR+SIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSY+QSQ LTEATQA+KV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQ+LP+GTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSM 780
           QFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN M
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 SP 783
           +P
Sbjct: 781 TP 782

BLAST of Lcy08g003980 vs. ExPASy TrEMBL
Match: A0A6J1JAN2 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111483261 PE=3 SV=1)

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 742/783 (94.76%), Postives = 761/783 (97.19%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI++  MDEK KRMRDLLSSFYSPDAS SGSS  SSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGSMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSK+GNVSESVYGASHE SV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEGSV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEA+RAYRVIFADSDRQLIKLAQDLVTKHF+A EQFIKKQIC+ADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFNAIEQFIKKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQVAVKQYVTC FSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCMFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+SIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+SYSQSQVL EATQA+KVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSYSQSQVLPEATQAEKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQ+LPQGTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTL EF+RLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFIRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS 780
           MQFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLI+AKLAKA+DQN 
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIKAKLAKAKDQNP 780

Query: 781 MSP 783
           MSP
Sbjct: 781 MSP 783

BLAST of Lcy08g003980 vs. ExPASy TrEMBL
Match: A0A6J1K9H7 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita maxima OX=3661 GN=LOC111492889 PE=3 SV=1)

HSP 1 Score: 1393.6 bits (3606), Expect = 0.0e+00
Identity = 740/782 (94.63%), Postives = 757/782 (96.80%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSST SSNRYASPLE INTTSFNPDQYMSI
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NL+GLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNA+DLSS L+I S KE N SES YGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDA EQFIKKQI +ADLLRVFGIIWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDACL DYSLKAAQVAVKQYVTCTFSRLLQ+ISDALT+VHTRKKE VQEYSLQ
Sbjct: 361 LLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQ 420

Query: 421 LALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFML 480
           LALEA KK VLQGSMDVLLDFR LLE+QSGLIINQR+SIVDWVQEGFQDFFR LVDRFML
Sbjct: 421 LALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRELVDRFML 480

Query: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSYSQSQVLTEA QADKVVAGLVLVLAQIS+FIEQTAI RITEEIAASFSGGG+R
Sbjct: 481 LSGKNNSYSQSQVLTEANQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEV+Q+L QGTRKHRRT SNGSTTSSRS PLREEKLNRS+ QRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNRSSAQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQT+NRSGFQQIQLDM
Sbjct: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDM 720

Query: 721 QFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSM 780
           QFLR PLKEIADDEA IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS+
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSI 780

Query: 781 SP 783
           SP
Sbjct: 781 SP 782

BLAST of Lcy08g003980 vs. ExPASy TrEMBL
Match: A0A6J1GBC0 (Vacuolar protein sorting-associated protein 51 homolog OS=Cucurbita moschata OX=3662 GN=LOC111452610 PE=3 SV=1)

HSP 1 Score: 1393.6 bits (3606), Expect = 0.0e+00
Identity = 739/782 (94.50%), Postives = 758/782 (96.93%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSST SSNRYASPLE INTTSFNPDQYMSI
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTGSSNRYASPLETINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQK+NL+GLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKANLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLK LQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKKLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNA+DLSS L+I S KE N SES YGA+HEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNADDLSSGLLIASPKERNDSESAYGAAHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEAVRAYRVIFADSD QLIKLAQDLVTKHFDA EQFIKKQI +ADLLRVFGIIWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDMQLIKLAQDLVTKHFDAAEQFIKKQIFAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDACL DYSLKAAQVAVKQYVTCTFSRLLQ+ISDALT+VHTRKKE VQEYSLQ
Sbjct: 361 LLLGEVLNDACLHDYSLKAAQVAVKQYVTCTFSRLLQNISDALTRVHTRKKEDVQEYSLQ 420

Query: 421 LALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFML 480
           LALEA KK VLQGSMDVLLDFR LLE+QSGLIINQR+SIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LALEAGKKAVLQGSMDVLLDFRLLLENQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQIS+FIEQTAI RITEEIAASFSGGG+R
Sbjct: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISIFIEQTAIHRITEEIAASFSGGGVR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEV+Q+L QGTRKHRRT SNGSTTSSRS PLREEKLN++N QRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVRQILLQGTRKHRRTNSNGSTTSSRSGPLREEKLNKTNAQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLE+HLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQT+NRSGFQQIQLDM
Sbjct: 661 LLESHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTYNRSGFQQIQLDM 720

Query: 721 QFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSM 780
           QFLR PLKEIADDEA IDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS+
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSI 780

Query: 781 SP 783
           SP
Sbjct: 781 SP 782

BLAST of Lcy08g003980 vs. NCBI nr
Match: XP_038887792.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887793.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887794.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887795.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida] >XP_038887796.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1409.0 bits (3646), Expect = 0.0e+00
Identity = 744/782 (95.14%), Postives = 762/782 (97.44%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI+DVPMDEKAKRMRDLLSSFY+PDASMS SS  SSNRY SPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYAPDASMSSSSMGSSNRYTSPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEE IA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEVIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQ+NAEDLSSALV GSSK+G+ SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQVNAEDLSSALVNGSSKDGSTSELVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQIC+ADLL VFGIIWTDV
Sbjct: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICAADLLLVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA LPDYSLKAAQVAVKQYV C FSRLLQDISDALTQVHTRKKEG QEYSLQ
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVKCKFSRLLQDISDALTQVHTRKKEGAQEYSLQ 420

Query: 421 LALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFML 480
           LALEA+KK VLQGSMDVLLDFRQLLED+SGLIINQR+SIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LALEATKKAVLQGSMDVLLDFRQLLEDKSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSYSQSQVLTEATQA+KV+AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYSQSQVLTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRIS+L TKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISILSTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQ+LPQGTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTLQEFVR QTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTLVKLSLKTLQEFVRFQTFNRSGFQQIQLDM 720

Query: 721 QFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSM 780
           QFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQNS+
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNSI 780

Query: 781 SP 783
           SP
Sbjct: 781 SP 782

BLAST of Lcy08g003980 vs. NCBI nr
Match: KAG6577400.1 (Vacuolar protein sorting-associated protein 51-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 744/783 (95.02%), Postives = 763/783 (97.45%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI++ PMDEK KRMRDLLSSFYSPDAS SGSS  SSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSK+GNVSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEA+RAYRVIFADSDRQLIKLAQDLVTKHFDA EQFI+KQIC+ADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDAIEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQVAVKQYVTCTFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+SIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+S+SQSQVL EATQA+KVVAGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEA+GSEVKQ+LPQGTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAIGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS 780
           MQFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN 
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDAIPLEPPILDKLIQAKLAKAKDQNP 780

Query: 781 MSP 783
            SP
Sbjct: 781 TSP 783

BLAST of Lcy08g003980 vs. NCBI nr
Match: XP_022929381.1 (vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929382.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929383.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata] >XP_022929384.1 vacuolar protein sorting-associated protein 51 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 745/783 (95.15%), Postives = 763/783 (97.45%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI++ PMDEK KRMRDLLSSFYSPDAS SGSS  SSNRYASPLEAINTTSFNPDQYMSI
Sbjct: 1   MEIDNGPMDEKTKRMRDLLSSFYSPDASTSGSSMGSSNRYASPLEAINTTSFNPDQYMSI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQ+HVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQKHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLL KVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLCKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFK YGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKVYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSK+GNVSESVYGASHEASV
Sbjct: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKDGNVSESVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REFAEA+RAYRVIFADSDRQLIKLAQDLVTKHFDATEQFI+KQIC+ADLLRVFGIIWTDV
Sbjct: 301 REFAEAIRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIRKQICAADLLRVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKK-EGVQEYSL 420
           LLLGEVLNDA LPDYSLKAAQVAVKQYVTCTFSRLLQDISDAL QVHTRKK EGVQEYSL
Sbjct: 361 LLLGEVLNDAGLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALAQVHTRKKEEGVQEYSL 420

Query: 421 QLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFM 480
           QLALEASKK VLQGSMDVLLDFRQLLEDQSGL I+QR+SIVDWVQEGFQDFFRALVDRFM
Sbjct: 421 QLALEASKKAVLQGSMDVLLDFRQLLEDQSGLTISQRDSIVDWVQEGFQDFFRALVDRFM 480

Query: 481 LLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           LLSGKN+S+SQSQVL EATQA+KVVAGLVLVLAQ+SVFIEQTAIPRITEEIAASFSGGG 
Sbjct: 481 LLSGKNSSFSQSQVLPEATQAEKVVAGLVLVLAQVSVFIEQTAIPRITEEIAASFSGGGT 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH
Sbjct: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARS 660
           MFVDLFLQELEAVGSEVKQ+LPQGTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARS
Sbjct: 601 MFVDLFLQELEAVGSEVKQILPQGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARS 660

Query: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLD 720
           QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTL EFVRLQTFNRSGFQQ+QLD
Sbjct: 661 QLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLLEFVRLQTFNRSGFQQVQLD 720

Query: 721 MQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNS 780
           MQFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN 
Sbjct: 721 MQFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDTIPLEPPILDKLIQAKLAKAKDQNP 780

Query: 781 MSP 783
            SP
Sbjct: 781 TSP 783

BLAST of Lcy08g003980 vs. NCBI nr
Match: XP_004139639.1 (vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >XP_011659140.1 vacuolar protein sorting-associated protein 51 homolog [Cucumis sativus] >KGN44805.2 hypothetical protein Csa_015985 [Cucumis sativus])

HSP 1 Score: 1399.8 bits (3622), Expect = 0.0e+00
Identity = 741/782 (94.76%), Postives = 761/782 (97.31%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGS T SSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L++ALV  SSK+GN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTTALVNASSKDGNSSELVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REF EAVRAYRVIFADSDRQLIKLAQDLVTKHFD+TEQFIKKQIC+ADLL VFGIIWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGIIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LL GEVLNDA L DYSLKAAQVAVKQYVT TFSRLLQDISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLFGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFML 480
           L LEASKK VLQGSMDVLL+FRQLLEDQSGLIINQR+SIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LELEASKKAVLQGSMDVLLEFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSY+QSQ LTEATQA+KV+AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVIAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVK+KEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKYKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQ+LP+GTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSM 780
           QFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN M
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 SP 783
           +P
Sbjct: 781 TP 782

BLAST of Lcy08g003980 vs. NCBI nr
Match: XP_008461504.1 (PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo] >XP_008461505.1 PREDICTED: vacuolar protein sorting-associated protein 51 homolog [Cucumis melo])

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 741/782 (94.76%), Postives = 759/782 (97.06%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           MEI+DVPMDEKAKRMRDLLSSFYSPDASMS S T SSNRYASPLEAINTTSFNPDQYM+I
Sbjct: 1   MEIDDVPMDEKAKRMRDLLSSFYSPDASMSSSPTGSSNRYASPLEAINTTSFNPDQYMNI 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           LVQKSNL+GLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM
Sbjct: 61  LVQKSNLEGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY
Sbjct: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
           ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIA+VLKNLQEKLFSDSESIQTRAEAAVL
Sbjct: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAVVLKNLQEKLFSDSESIQTRAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGASHEASV 300
           LKQLDFPVDSLK KLLEKLEQSTLDLQLNAE+L+SALV  SSK+GN SE VYGASHEASV
Sbjct: 241 LKQLDFPVDSLKVKLLEKLEQSTLDLQLNAENLTSALVNASSKDGNTSELVYGASHEASV 300

Query: 301 REFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTDV 360
           REF EAVRAYRVIFADSDRQLIKLAQDLVTKHFD+TEQFIKKQIC+ADLL VFG IWTDV
Sbjct: 301 REFTEAVRAYRVIFADSDRQLIKLAQDLVTKHFDSTEQFIKKQICAADLLLVFGTIWTDV 360

Query: 361 LLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420
           LLLGEVLNDA L DYSLKAAQVAVKQYVT TFSRLLQDISDALTQVHTRKKEGVQEYSLQ
Sbjct: 361 LLLGEVLNDAGLLDYSLKAAQVAVKQYVTFTFSRLLQDISDALTQVHTRKKEGVQEYSLQ 420

Query: 421 LALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFML 480
           L LEASKK VLQGSMD+LLDFRQLLEDQSGLIINQR+SIVDWVQEGFQDFFRALVDRFML
Sbjct: 421 LELEASKKAVLQGSMDILLDFRQLLEDQSGLIINQRDSIVDWVQEGFQDFFRALVDRFML 480

Query: 481 LSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540
           LSGKNNSY+QSQ LTEATQA+KV AGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR
Sbjct: 481 LSGKNNSYTQSQALTEATQAEKVNAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGIR 540

Query: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600
           GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM
Sbjct: 541 GYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVHM 600

Query: 601 FVDLFLQELEAVGSEVKQVLPQGTRKHRRTESNGSTTSSRSNPLREEKLNRSNTQRARSQ 660
           FVDLFLQELEAVGSEVKQ+LP+GTRKHRRT+SNGSTTSSRSNPLREEKLNRSNTQRARSQ
Sbjct: 601 FVDLFLQELEAVGSEVKQILPEGTRKHRRTDSNGSTTSSRSNPLREEKLNRSNTQRARSQ 660

Query: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720
           LLETHLAKLFKQKIEIFTRVEFTQGSVVTT VKLSLKTLQEFVRLQTFNRSGFQQIQLDM
Sbjct: 661 LLETHLAKLFKQKIEIFTRVEFTQGSVVTTIVKLSLKTLQEFVRLQTFNRSGFQQIQLDM 720

Query: 721 QFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARDQNSM 780
           QFLR PLKEIADDEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAKA+DQN M
Sbjct: 721 QFLRTPLKEIADDEAAIDFLLDEVIVAASERCLDSIPLEPPILDKLIQAKLAKAKDQNPM 780

Query: 781 SP 783
           +P
Sbjct: 781 TP 782

BLAST of Lcy08g003980 vs. TAIR 10
Match: AT4G02030.1 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 984.2 bits (2543), Expect = 6.2e-287
Identity = 529/787 (67.22%), Postives = 635/787 (80.69%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           M  E  PMDEKAKRMRDLLSSFY+PD S+S S +S +    +  + IN+TSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSIN----ASFDNINSTSFDADQYMDL 60

Query: 61  LVQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMNNNIVGMETNM 120
           +++KSNL+ LLQRHV+MAAEIKNLDTDLQMLVYENYNKFISATDTIKRM +NI GME NM
Sbjct: 61  MIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNM 120

Query: 121 EQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLGKCIKTEAY 180
           +QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARL KCIK+EAY
Sbjct: 121 DQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAY 180

Query: 181 ADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAIVLKNLQEKLFSDSESIQTRAEAAVL 240
            DAVRFYTGAMPI K YGD+SFQDC+RASEEAI I++KNLQ KLFSDSESIQ RAEAAVL
Sbjct: 181 GDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVL 240

Query: 241 LKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSSALVIGSSKEGNVSESVYGAS-HEAS 300
           LKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S+ +    S     S   + A  HE +
Sbjct: 241 LKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDA 300

Query: 301 VREFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFDATEQFIKKQICSADLLRVFGIIWTD 360
           VR F+EA+RAYR IF DS+ +L KLA+ L   HF+  E +IKK++ +AD L +F I+W D
Sbjct: 301 VRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWED 360

Query: 361 VLLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSRLLQDISDALTQVHTRKKEGVQEYSL 420
           V+L+ EVL +A L D S +AAQV +KQ+V   FS L QDISD L +    +KE V+   L
Sbjct: 361 VVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELL 420

Query: 421 QLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIINQRNSIVDWVQEGFQDFFRALVDRFM 480
           ++ LEAS+K VLQG+ ++  DFRQLL++++G+ I  ++ I  W+Q+G QDFFR+L  +F+
Sbjct: 421 KVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFL 480

Query: 481 LLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQISVFIEQTAIPRITEEIAASFSGGGI 540
           +LSGK +S +      E   +DK+ AGL+LVLAQ+SVFIEQ  IPR+TEEIAASFSGG  
Sbjct: 481 VLSGKTSSSND----IEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNS 540

Query: 541 RGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQRISVLLTKRFRTPNWVKHKEPREVH 600
           + +E GPAF+P E+CR+F AA EK L  YI+ R+Q++SVLL KRF+TPNWVKHKEPREVH
Sbjct: 541 QAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVH 600

Query: 601 MFVDLFLQELEAVGSEVKQVLPQGT-RKHRRTESNGS--TTSSRSNPLREEKLNRSNTQR 660
           M+VD+FL ELE VG EVKQVLPQGT RKH+RT+SNGS  TTSSRSN L  +K+ RSN+QR
Sbjct: 601 MYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQR 660

Query: 661 ARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTAVKLSLKTLQEFVRLQTFNRSGFQQI 720
           ARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT VKL LK+LQE+VRLQTFNRSGFQQI
Sbjct: 661 ARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQI 720

Query: 721 QLDMQFLRAPLKEIADDEALIDFLLDEVIVAASERCLDPIPLEPPILDKLIQAKLAKARD 780
           QLD+QFL+APLKE  +DEA IDFLLDEVIVAASERCLD IPLEPPILDKLIQAKLAK+++
Sbjct: 721 QLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKE 779

Query: 781 QNSMSPS 784
            N+ + S
Sbjct: 781 HNNNTVS 779

BLAST of Lcy08g003980 vs. TAIR 10
Match: AT4G02030.2 (Vps51/Vps67 family (components of vesicular transport) protein )

HSP 1 Score: 970.3 bits (2507), Expect = 9.2e-283
Identity = 529/812 (65.15%), Postives = 635/812 (78.20%), Query Frame = 0

Query: 1   MEIEDVPMDEKAKRMRDLLSSFYSPDASMSGSSTSSSNRYASPLEAINTTSFNPDQYMSI 60
           M  E  PMDEKAKRMRDLLSSFY+PD S+S S +S +    +  + IN+TSF+ DQYM +
Sbjct: 1   MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSIN----ASFDNINSTSFDADQYMDL 60

Query: 61  L-------------------------VQKSNLDGLLQRHVEMAAEIKNLDTDLQMLVYEN 120
           +                         ++KSNL+ LLQRHV+MAAEIKNLDTDLQMLVYEN
Sbjct: 61  MIRLFDLGLSDLPAMVDSSELNWVYEIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYEN 120

Query: 121 YNKFISATDTIKRMNNNIVGMETNMEQLLEKILSVQSRSDGVNTSLFEKREHIEKLHRTR 180
           YNKFISATDTIKRM +NI GME NM+QLL+KI+SVQS+SDGVNTSLFEKREHIEKLHRTR
Sbjct: 121 YNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTR 180

Query: 181 NLLRKVQFIYDLPARLGKCIKTEAYADAVRFYTGAMPIFKAYGDSSFQDCKRASEEAIAI 240
           NLLRKVQFIYDLPARL KCIK+EAY DAVRFYTGAMPI K YGD+SFQDC+RASEEAI I
Sbjct: 181 NLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEI 240

Query: 241 VLKNLQEKLFSDSESIQTRAEAAVLLKQLDFPVDSLKAKLLEKLEQSTLDLQLNAEDLSS 300
           ++KNLQ KLFSDSESIQ RAEAAVLLKQLD PVDSLKAKLLEKLEQS   LQ+  E+ S+
Sbjct: 241 IIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEAST 300

Query: 301 ALVIGSSKEGNVSESVYGAS-HEASVREFAEAVRAYRVIFADSDRQLIKLAQDLVTKHFD 360
            +    S     S   + A  HE +VR F+EA+RAYR IF DS+ +L KLA+ L   HF+
Sbjct: 301 LVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFE 360

Query: 361 ATEQFIKKQICSADLLRVFGIIWTDVLLLGEVLNDACLPDYSLKAAQVAVKQYVTCTFSR 420
             E +IKK++ +AD L +F I+W DV+L+ EVL +A L D S +AAQV +KQ+V   FS 
Sbjct: 361 YMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSH 420

Query: 421 LLQDISDALTQVHTRKKEGVQEYSLQLALEASKKTVLQGSMDVLLDFRQLLEDQSGLIIN 480
           L QDISD L +    +KE V+   L++ LEAS+K VLQG+ ++  DFRQLL++++G+ I 
Sbjct: 421 LQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIK 480

Query: 481 QRNSIVDWVQEGFQDFFRALVDRFMLLSGKNNSYSQSQVLTEATQADKVVAGLVLVLAQI 540
            ++ I  W+Q+G QDFFR+L  +F++LSGK +S +      E   +DK+ AGL+LVLAQ+
Sbjct: 481 MKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSND----IEGKSSDKIHAGLILVLAQL 540

Query: 541 SVFIEQTAIPRITEEIAASFSGGGIRGYEYGPAFVPAEICRMFRAAGEKFLHLYINMRSQ 600
           SVFIEQ  IPR+TEEIAASFSGG  + +E GPAF+P E+CR+F AA EK L  YI+ R+Q
Sbjct: 541 SVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQ 600

Query: 601 RISVLLTKRFRTPNWVKHKEPREVHMFVDLFLQELEAVGSEVKQVLPQGT-RKHRRTESN 660
           ++SVLL KRF+TPNWVKHKEPREVHM+VD+FL ELE VG EVKQVLPQGT RKH+RT+SN
Sbjct: 601 KVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSN 660

Query: 661 GS--TTSSRSNPLREEKLNRSNTQRARSQLLETHLAKLFKQKIEIFTRVEFTQGSVVTTA 720
           GS  TTSSRSN L  +K+ RSN+QRARSQL ETHLAKLFKQK+EIFT+VEFTQ SVVTT 
Sbjct: 661 GSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTT 720

Query: 721 VKLSLKTLQEFVRLQTFNRSGFQQIQLDMQFLRAPLKEIADDEALIDFLLDEVIVAASER 780
           VKL LK+LQE+VRLQTFNRSGFQQIQLD+QFL+APLKE  +DEA IDFLLDEVIVAASER
Sbjct: 721 VKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASER 780

Query: 781 CLDPIPLEPPILDKLIQAKLAKARDQNSMSPS 784
           CLD IPLEPPILDKLIQAKLAK+++ N+ + S
Sbjct: 781 CLDVIPLEPPILDKLIQAKLAKSKEHNNNTVS 804