Lcy08g001950 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy08g001950
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Cysteine protease
Location	Chr08: 2528096 .. 2530780 (-)
RNA-Seq Expression	Lcy08g001950
Synteny	Lcy08g001950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTCCATTGGCGATCCAATCTCTTCTTCTCAACTTCCCTATAAAATCACGAATCCCCTTAACTTTCCCTTCATACCCATTTTCGTTTCTACCCAAACCCACCAAAACAATGGCGATTTCCCCCGCTTTCTCCTTCTCCGTCGTCGCTTTTCTACCGCTGTTCCTCTGTTTCTCCTCATTTTCATCGGCTTCCGATTCATCGAGCTTCTCCATCATCGATGAAAACGCGAAACACCACTTGGGTATCCCCGATATCGCCGATTCCCAAGTCGGATCCCCCCAGCGGACTGAGGAGGAGGTTGCAGCTATCTACGAGTCGTGGTTGGTCCACCATAGAAAAGCTTACAACGCTCTCGGCGAGAAGGAGAGGCGGTTTCAGATTTTCAAGGATAATCTGAGGTTCATTGATGAACATAACAGGGAATCGCGGAGTTATAAGGTGGGATTGAACCGTTTCGCCGATCTCACCAATGAGGAGTATCGGACCAGGTTTTTGGGTGGCCGATTCTCGCGGAAGCCGCGGCTCTCCGCCGTCAAGAGCCGTAGGTACGCGGCGGCGCTCGGCGATGATCTGCCGGATCATGTCGATTGGAGAAAGAAGGGTGCTGTTGCGGATGTTAAAGATCAAGGACAGTGTGGTGAGTTTTCTTGTTCTGATCCGATGAGAATGGTGGTTGGATTCTATGTTTTCCTTTTGTTTTCTCATCTGGTCTTCGAATTTTTTGACTTTTGTGAACTACCGATTATTATGGATTTGCATTCTGTTTGAATTGATGCTGATTTCCCCAGGAAATTTGTTACGAGATCCATATTTATCGGAAGCATCTGTCTAAAATAGATTCATTAGATATTGGTTAACGCTTAACGACCCTGATCATAATTTGTATCTGTTCAAAATAGCTTCTTTTAATCACTGACGATTGGTTGATGGTCATCTCTCACATCAGAAGCTTTTCTGGAAAAACTTGTTTTCCAAGAAACCTTTTATCTCGGAGGATTGTTTGAATACTGGCTTTTCTACCCGAGAATCCAGTTCTTTCTACACTGCTTGTACAGATTTTCATGGATGATTAACGTAGCTGGGTTATGTTTTGAATGAAGGGAGCTGCTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATCGCCACCGGTGAATTGATCACTCTGTCGGAGCAGGAGCTTGTGGACTGCGATAGATCGTATAACATGGGGTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATCGACACTGAGGAAGATTACCCTTACAAGGGCCGTGATGGCGCTTGCGATCCCAACCGGGTTTGTCTCGAGCTGCTTCGTTCAATAGTTCAATTCATTTATTGAACCCGACATTCTAATTTGACTTGTTTTGTCAACAGAGGAATGCTAAGGTTGTCACCATTGATGGATATGAAGATGTTCCTGAGAATGATGAGATCTCCTTGAAAAAGGCTGTGGCAGGTCAGCCTGTTAGTGTTGCCATTGAAGCTGGTGGCAGGGCCTTCCAACTTTACCAATCGGTAAGCTCTTCAACTTTGAACAACTTCCTGAATGTTGTTTTGGTTTTGACATCGCTGGCTTCAAATATGATAGCTGTTTCAAATGCAACACAACATGTGTTTATGCATCATTCAGGCATTAGGAAATGATAGCTAGCTGTTTCAAACCTTTTTTTTAACACAATATGTTGAGGGGAAGATTTGAACCTCTGATCTCTTGACGTGGGTATGTTTATGCATGATTCAGGGTGTCTTCACTGGACGTTGTGGAACCGATCTCGATCACGGTGTTGTTGCTGTTGGATATGGTACAGAAGGTGGTGTAGATTACTGGATTGTGAGGAATTCCTGGGGCAAAAACTGGGGAGAAAATGGCTATATCAGGTTGGAGAGAAATGTGGCCAACATTACCACAGGCAAGTGTGGTATAGCCATAGAGCCTTCATACCCCATCAAGACAGGAAAGAACCCTCCAAAACCTGCTCCTTCTCCACCCTCCCCTGTCAAGCCCCCGACCGAATGCGACGAGTACTTCTCGTGCCAGGAAGGGAGTACCTGCTGCTGTATCTATGAGTATGGAAATACCTGCTTTGGTTGGGGATGCTGCCCGCTCGAGTCGGCGACCTGCTGCGATGACCATTACAGCTGCTGCCCCCATGAATATCCCATCTGTGACCTTGAGGCAGGGACATGCCGTTTGGTAAGTCACATCACATAGTTTTGTAACCAATGCTGGTCATGTTTGATACAATCTGATCATTGTCAATGGATGAAATGCAGAGCAAAGGCAGCTCGATCGGAGTCGATCTGCTGAAGCGTGTTCCTGCTAAACGAACAAGGGGCATTCAGAAGCTTGGGAAGGCGGTCTTTGGTGCTTGAGACTATCTGCAATTCTTGTTGGGGCCATTAGAGCTCAAGAGTACAGAAAACTATGACAAGTAGAAGCTTTTGATGGAGACCCCCATTTATTTATCTTCTTTACATTATTGAACTTTGATTATGTGTTGTTTCCCTTGCTGAAAAACACTGATGGTGACTTTGTACATAAAGTTTCCTCATGTGAAAACTTGTAACAAACTTCATCTCATTTGCTTTGAAGATAAATTGTATATATTTATCAATTCTAACTCTTGGATGCGGTTGATGTT

mRNA sequence

ACTTCCATTGGCGATCCAATCTCTTCTTCTCAACTTCCCTATAAAATCACGAATCCCCTTAACTTTCCCTTCATACCCATTTTCGTTTCTACCCAAACCCACCAAAACAATGGCGATTTCCCCCGCTTTCTCCTTCTCCGTCGTCGCTTTTCTACCGCTGTTCCTCTGTTTCTCCTCATTTTCATCGGCTTCCGATTCATCGAGCTTCTCCATCATCGATGAAAACGCGAAACACCACTTGGGTATCCCCGATATCGCCGATTCCCAAGTCGGATCCCCCCAGCGGACTGAGGAGGAGGTTGCAGCTATCTACGAGTCGTGGTTGGTCCACCATAGAAAAGCTTACAACGCTCTCGGCGAGAAGGAGAGGCGGTTTCAGATTTTCAAGGATAATCTGAGGTTCATTGATGAACATAACAGGGAATCGCGGAGTTATAAGGTGGGATTGAACCGTTTCGCCGATCTCACCAATGAGGAGTATCGGACCAGGTTTTTGGGTGGCCGATTCTCGCGGAAGCCGCGGCTCTCCGCCGTCAAGAGCCGTAGGTACGCGGCGGCGCTCGGCGATGATCTGCCGGATCATGTCGATTGGAGAAAGAAGGGTGCTGTTGCGGATGTTAAAGATCAAGGACAGTGTGGGAGCTGCTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATCGCCACCGGTGAATTGATCACTCTGTCGGAGCAGGAGCTTGTGGACTGCGATAGATCGTATAACATGGGGTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATCGACACTGAGGAAGATTACCCTTACAAGGGCCGTGATGGCGCTTGCGATCCCAACCGGAGGAATGCTAAGGTTGTCACCATTGATGGATATGAAGATGTTCCTGAGAATGATGAGATCTCCTTGAAAAAGGCTGTGGCAGGTCAGCCTGTTAGTGTTGCCATTGAAGCTGGTGGCAGGGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGACGTTGTGGAACCGATCTCGATCACGGTGTTGTTGCTGTTGGATATGGTACAGAAGGTGGTGTAGATTACTGGATTGTGAGGAATTCCTGGGGCAAAAACTGGGGAGAAAATGGCTATATCAGGTTGGAGAGAAATGTGGCCAACATTACCACAGGCAAGTGTGGTATAGCCATAGAGCCTTCATACCCCATCAAGACAGGAAAGAACCCTCCAAAACCTGCTCCTTCTCCACCCTCCCCTGTCAAGCCCCCGACCGAATGCGACGAGTACTTCTCGTGCCAGGAAGGGAGTACCTGCTGCTGTATCTATGAGTATGGAAATACCTGCTTTGGTTGGGGATGCTGCCCGCTCGAGTCGGCGACCTGCTGCGATGACCATTACAGCTGCTGCCCCCATGAATATCCCATCTGTGACCTTGAGGCAGGGACATGCCGTTTGAGCAAAGGCAGCTCGATCGGAGTCGATCTGCTGAAGCGTGTTCCTGCTAAACGAACAAGGGGCATTCAGAAGCTTGGGAAGGCGGTCTTTGGTGCTTGAGACTATCTGCAATTCTTGTTGGGGCCATTAGAGCTCAAGAGTACAGAAAACTATGACAAGTAGAAGCTTTTGATGGAGACCCCCATTTATTTATCTTCTTTACATTATTGAACTTTGATTATGTGTTGTTTCCCTTGCTGAAAAACACTGATGGTGACTTTGTACATAAAGTTTCCTCATGTGAAAACTTGTAACAAACTTCATCTCATTTGCTTTGAAGATAAATTGTATATATTTATCAATTCTAACTCTTGGATGCGGTTGATGTT

Coding sequence (CDS)

ATGGCGATTTCCCCCGCTTTCTCCTTCTCCGTCGTCGCTTTTCTACCGCTGTTCCTCTGTTTCTCCTCATTTTCATCGGCTTCCGATTCATCGAGCTTCTCCATCATCGATGAAAACGCGAAACACCACTTGGGTATCCCCGATATCGCCGATTCCCAAGTCGGATCCCCCCAGCGGACTGAGGAGGAGGTTGCAGCTATCTACGAGTCGTGGTTGGTCCACCATAGAAAAGCTTACAACGCTCTCGGCGAGAAGGAGAGGCGGTTTCAGATTTTCAAGGATAATCTGAGGTTCATTGATGAACATAACAGGGAATCGCGGAGTTATAAGGTGGGATTGAACCGTTTCGCCGATCTCACCAATGAGGAGTATCGGACCAGGTTTTTGGGTGGCCGATTCTCGCGGAAGCCGCGGCTCTCCGCCGTCAAGAGCCGTAGGTACGCGGCGGCGCTCGGCGATGATCTGCCGGATCATGTCGATTGGAGAAAGAAGGGTGCTGTTGCGGATGTTAAAGATCAAGGACAGTGTGGGAGCTGCTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATCGCCACCGGTGAATTGATCACTCTGTCGGAGCAGGAGCTTGTGGACTGCGATAGATCGTATAACATGGGGTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATCGACACTGAGGAAGATTACCCTTACAAGGGCCGTGATGGCGCTTGCGATCCCAACCGGAGGAATGCTAAGGTTGTCACCATTGATGGATATGAAGATGTTCCTGAGAATGATGAGATCTCCTTGAAAAAGGCTGTGGCAGGTCAGCCTGTTAGTGTTGCCATTGAAGCTGGTGGCAGGGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGACGTTGTGGAACCGATCTCGATCACGGTGTTGTTGCTGTTGGATATGGTACAGAAGGTGGTGTAGATTACTGGATTGTGAGGAATTCCTGGGGCAAAAACTGGGGAGAAAATGGCTATATCAGGTTGGAGAGAAATGTGGCCAACATTACCACAGGCAAGTGTGGTATAGCCATAGAGCCTTCATACCCCATCAAGACAGGAAAGAACCCTCCAAAACCTGCTCCTTCTCCACCCTCCCCTGTCAAGCCCCCGACCGAATGCGACGAGTACTTCTCGTGCCAGGAAGGGAGTACCTGCTGCTGTATCTATGAGTATGGAAATACCTGCTTTGGTTGGGGATGCTGCCCGCTCGAGTCGGCGACCTGCTGCGATGACCATTACAGCTGCTGCCCCCATGAATATCCCATCTGTGACCTTGAGGCAGGGACATGCCGTTTGAGCAAAGGCAGCTCGATCGGAGTCGATCTGCTGAAGCGTGTTCCTGCTAAACGAACAAGGGGCATTCAGAAGCTTGGGAAGGCGGTCTTTGGTGCTTGA

Protein sequence

MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLTNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWGCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRGIQKLGKAVFGA

Homology

[Close]

BLAST of Lcy08g001950 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 6.5e-183
Identity = 309/455 (67.91%), Postives = 363/455 (79.78%), Query Frame = 0


Query: 17  LFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVHHR 76
           LFL   + SSA D    SII  + KH +       S  G   R+E EV +IYE+WLV H 
Sbjct: 11  LFLAMVAVSSAVD---MSIISYDEKHGV-------STTGG--RSEAEVMSIYEAWLVKHG 70

Query: 77  KA--YNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLTNEEYRTRFLGGRFS 136
           KA   N+L EK+RRF+IFKDNLRF+DEHN ++ SY++GL RFADLTN+EYR+++LG +  
Sbjct: 71  KAQSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKME 130

Query: 137 RKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTVAAVEGINQI 196
           +K       S RY A +GD+LP+ +DWRKKGAVA+VKDQG CGSCWAFST+ AVEGINQI
Sbjct: 131 KKGERRT--SLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQI 190

Query: 197 ATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYKGRDGACDPN 256
            TG+LITLSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDT++DYPYKG DG CD  
Sbjct: 191 VTGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQI 250

Query: 257 RRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLD 316
           R+NAKVVTID YEDVP   E SLKKAVA QP+S+AIEAGGRAFQLY SG+F G CGT LD
Sbjct: 251 RKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLD 310

Query: 317 HGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPIKTG 376
           HGVVAVGYGTE G DYWIVRNSWGK+WGE+GY+R+ RN+A+ ++GKCGIAIEPSYPIK G
Sbjct: 311 HGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIAS-SSGKCGIAIEPSYPIKNG 370

Query: 377 KNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWGCCPLESATCCDDHY 436
           +NPP P PSPPSP+KPPT+CD Y++C E +TCCC++EYG  CF WGCCPLE+ATCCDD+Y
Sbjct: 371 ENPPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNY 430

Query: 437 SCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPA 470
           SCCPHEYP+CDL+ GTC LSK S   V  LKR PA
Sbjct: 431 SCCPHEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

[Close]

BLAST of Lcy08g001950 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.5e-179
Identity = 308/458 (67.25%), Postives = 350/458 (76.42%), Query Frame = 0


Query: 17  LFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVHHR 76
           L L     S A D    SII  +  HH+              R++ EV  IYE+W+V H 
Sbjct: 11  LLLAMIGVSYAMD---MSIISYDENHHI---------TTETSRSDSEVERIYEAWMVEHG 70

Query: 77  KA---YNALG-EKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLTNEEYRTRFLGGR 136
           K     N LG EK++RF+IFKDNLRFIDEHN ++ SYK+GL RFADLTNEEYR+ +LG  
Sbjct: 71  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGA- 130

Query: 137 FSRKPRLSAVK-SRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTVAAVEGI 196
              KP    +K S RY A +GD LPD VDWRK+GAVADVKDQG CGSCWAFST+ AVEGI
Sbjct: 131 ---KPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGI 190

Query: 197 NQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYKGRDGAC 256
           N+I TG+LI+LSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDTE DYPYK  DG C
Sbjct: 191 NKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRC 250

Query: 257 DPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVFTGRCGT 316
           D NR+NAKVVTID YEDVPEN E SLKKA+A QP+SVAIEAGGRAFQLY SGVF G CGT
Sbjct: 251 DQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGT 310

Query: 317 DLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPI 376
           +LDHGVVAVGYGTE G DYWIVRNSWG  WGE+GYI++ RN+    TGKCGIA+E SYPI
Sbjct: 311 ELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEASYPI 370

Query: 377 KTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWGCCPLESATCCD 436
           K G+NPP P PSPPSP+KPPT CD+YFSC E +TCCC+Y+YG  CFGWGCCPLE+ATCCD
Sbjct: 371 KKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCD 430

Query: 437 DHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPA 470
           D+ SCCPHEYP+CD+  GTC +SK S   V  LKR PA
Sbjct: 431 DNSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPA 451

[Close]

BLAST of Lcy08g001950 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 618.6 bits (1594), Expect = 5.9e-176
Identity = 288/423 (68.09%), Postives = 338/423 (79.91%), Query Frame = 0


Query: 52  SQVGSPQRTEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRES----R 111
           S V   +R+EEE   +Y  W   H K+YNA+GE+ERR+  F+DNLR+IDEHN  +     
Sbjct: 24  SIVSYGERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVH 83

Query: 112 SYKVGLNRFADLTNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAV 171
           S+++GLNRFADLTNEEYR  +LG R   KPR     S RY AA  + LP+ VDWR KGAV
Sbjct: 84  SFRLGLNRFADLTNEEYRDTYLGLR--NKPRRERKVSDRYLAADNEALPESVDWRTKGAV 143

Query: 172 ADVKDQGQCGSCWAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAF 231
           A++KDQG CGSCWAFS +AAVEGINQI TG+LI+LSEQELVDCD SYN GCNGGLMDYAF
Sbjct: 144 AEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAF 203

Query: 232 QFIIDNGGIDTEEDYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVS 291
            FII+NGGIDTE+DYPYKG+D  CD NR+NAKVVTID YEDV  N E SL+KAVA QPVS
Sbjct: 204 DFIINNGGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVS 263

Query: 292 VAIEAGGRAFQLYQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYI 351
           VAIEAGGRAFQLY SG+FTG+CGT LDHGV AVGYGTE G DYWIVRNSWGK+WGE+GY+
Sbjct: 264 VAIEAGGRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYV 323

Query: 352 RLERNVANITTGKCGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCC 411
           R+ERN+   ++GKCGIA+EPSYP+K G+NPP P P+PPSP  PPT CD Y++C + +TCC
Sbjct: 324 RMERNI-KASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCC 383

Query: 412 CIYEYGNTCFGWGCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRV 471
           CIYEYG  C+ WGCCPLE ATCCDDHYSCCPHEYPIC+++ GTC ++K S + V  LKR 
Sbjct: 384 CIYEYGKYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTCLMAKDSPLAVKALKRT 443

[Close]

BLAST of Lcy08g001950 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.1e-156
Identity = 267/417 (64.03%), Postives = 314/417 (75.30%), Query Frame = 0


Query: 59  RTEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNR-ESRSYKVGLNRFA 118
           R E E   +YE WLV +RK YN LGEKERRF+IFKDNL+F++EH+   +R+Y+VGL RFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 119 DLTNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCG 178
           DLTN+E+R  +L  +  R      VK  +Y   +GD LPD +DWR KGAV  VKDQG CG
Sbjct: 94  DLTNDEFRAIYLRSKMER--TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCG 153

Query: 179 SCWAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGID 238
           SCWAFS + AVEGINQI TGELI+LSEQELVDCD SYN GC GGLMDYAF+FII+NGGID
Sbjct: 154 SCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGID 213

Query: 239 TEEDYPYKGRD-GACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRA 298
           TEEDYPY   D   C+ +++N +VVTIDGYEDVP+NDE SLKKA+A QP+SVAIEAGGRA
Sbjct: 214 TEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRA 273

Query: 299 FQLYQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANI 358
           FQLY SGVFTG CGT LDHGVVAVGYG+EGG DYWIVRNSWG NWGE+GY +LERN+   
Sbjct: 274 FQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE- 333

Query: 359 TTGKCGIAIEPSYPIK-TGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNT 418
           ++GKCG+A+  SYP K +G NPPKP      P   P  CD+  +C   STCCC+YEY   
Sbjct: 334 SSGKCGVAMMASYPTKSSGSNPPKP------PAPSPVVCDKSNTCPAKSTCCCLYEYNGK 393

Query: 419 CFGWGCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRT 473
           C+ WGCCP ESATCCDD  SCCP  YP+CDL+A TCR+   S + +  L R PA  T
Sbjct: 394 CYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIAT 441

[Close]

BLAST of Lcy08g001950 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 540.4 bits (1391), Expect = 2.1e-152
Identity = 276/465 (59.35%), Postives = 331/465 (71.18%), Query Frame = 0


Query: 18  FLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVHH-R 77
           FL      +A+ +   SII  NA+H  G   + +        TE E  A Y+ WL  +  
Sbjct: 10  FLLLLIVGAATAAPDMSIISYNAEH--GARGLEEGP------TEAEARAAYDLWLAENGG 69

Query: 78  KAYNAL-GEKERRFQIFKDNLRFIDEHNR---ESRSYKVGLNRFADLTNEEYRTRFLGGR 137
            + NAL GE ERRF +F DNL+F+D HN    E   +++G+NRFADLTNEE+R  FLG +
Sbjct: 70  GSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATFLGAK 129

Query: 138 FSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTVAAVEGIN 197
            + + R +     RY     ++LP+ VDWR+KGAVA VK+QGQCGSCWAFS V+ VE IN
Sbjct: 130 VAERSRAA---GERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESIN 189

Query: 198 QIATGELITLSEQELVDCD-RSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYKGRDGAC 257
           Q+ TGE+ITLSEQELV+C     N GCNGGLMD AF FII NGGIDTE+DYPYK  DG C
Sbjct: 190 QLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAVDGKC 249

Query: 258 DPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVFTGRCGT 317
           D NR NAKVV+IDG+EDVP+NDE SL+KAVA QPVSVAIEAGGR FQLY SGVF+GRCGT
Sbjct: 250 DINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSGRCGT 309

Query: 318 DLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPI 377
            LDHGVVAVGYGT+ G DYWIVRNSWG  WGE+GY+R+ERN+ N+TTGKCGIA+  SYP 
Sbjct: 310 SLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNI-NVTTGKCGIAMMASYPT 369

Query: 378 KTGKNPPKPAPSPPSPVKPPTE------CDEYFSCQEGSTCCCIYEYGNTCFGWGCCPLE 437
           K+G NPPKP+P+PP+P  PP        CD+ FSC  GSTCCC + + N C  WGCCP+E
Sbjct: 370 KSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGCCPVE 429

Query: 438 SATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAK 471
            ATCC DH SCCP +YP+C+  AGTC  SK S + V  LKR  AK
Sbjct: 430 GATCCKDHASCCPPDYPVCNTRAGTCSASKNSPLSVKALKRTLAK 462

[Close]

BLAST of Lcy08g001950 vs. ExPASy TrEMBL
Match: A0A6J1JKG6 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111486001 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.8e-247
Identity = 420/486 (86.42%), Postives = 439/486 (90.33%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS  FSFS+VAFL LFLCFSS S ASDSSSFSIIDENAKHHL      D Q+GSP R+
Sbjct: 1   MAISTTFSFSIVAFLALFLCFSSSSLASDSSSFSIIDENAKHHL------DVQIGSPHRS 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVH+ KAYNALGEKERRF+IFKDNLRFIDEHNRESRSYKVGLNRFADL+
Sbjct: 61  DEEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLS 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR  FL GR SRK RLSA KSRRYA  LGDDLPDHVDWRKKGAV DVKDQGQCGSCW
Sbjct: 121 NEEYRAMFLSGRLSRKNRLSATKSRRYAITLGDDLPDHVDWRKKGAVTDVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE
Sbjct: 181 AFSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDG CDPNRRN+K VTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGTCDPNRRNSKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVF G CGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGENGYI+LERNV NITTGK
Sbjct: 301 QSGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIAIE SYP KTGKNPPKPAPSPPSPV PP +CDEYFSCQEG+TCCCIY+YG+TCFGWG
Sbjct: 361 CGIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRG-IQKLG 480
           CCPLESATCCDDHYSCCPHEYPICDL+AGTC  SKGS+ GV LLKRVPAK  RG +QKLG
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTTGVKLLKRVPAKYNRGCVQKLG 480

Query: 481 KAVFGA 486
           K   GA
Sbjct: 481 KMSIGA 480

[Close]

BLAST of Lcy08g001950 vs. ExPASy TrEMBL
Match: A0A6J1EI11 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111434545 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 419/486 (86.21%), Postives = 439/486 (90.33%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS  F FS+V FLPLFLCFSS SSASDSSSFSIIDENA+HHL      D Q+GS QR+
Sbjct: 1   MAISTTFCFSIVVFLPLFLCFSSSSSASDSSSFSIIDENARHHL------DIQIGSSQRS 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVH+ KAYNALGEKERRF+IFKDNLRFIDEHNRESRSYKVGLNRFADL+
Sbjct: 61  DEEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLS 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR  FL GR SRK RLSA KSRRYA ALGDDLPDHVDWRKKGAV DVKDQGQCGSCW
Sbjct: 121 NEEYRAMFLSGRLSRKNRLSATKSRRYAIALGDDLPDHVDWRKKGAVTDVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE
Sbjct: 181 AFSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDG CDP R+NAK VTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGTCDPIRKNAKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVF G CGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGENGYI+LERNV NITTGK
Sbjct: 301 QSGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIAIE SYP KTGKNPPKPAPSPPSPV PP +CDEYFSCQEG+TCCCIY+YG+TCFGWG
Sbjct: 361 CGIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRG-IQKLG 480
           CCPLESATCCDDHYSCCPHEYPICDL+AGTC  SKGS+ GV+LLKRVPAK  RG  QKLG
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTSGVELLKRVPAKYNRGCAQKLG 480

Query: 481 KAVFGA 486
           K   GA
Sbjct: 481 KMSIGA 480

[Close]

BLAST of Lcy08g001950 vs. ExPASy TrEMBL
Match: A0A6J1D149 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111016644 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.1e-245
Identity = 408/486 (83.95%), Postives = 442/486 (90.95%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQ-R 60
           MAI  +FSF ++A L LFLCF SFSSASDSSSFSIIDENAKHHLG PDIA S  G+P  R
Sbjct: 1   MAIFSSFSFPILASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLR 60

Query: 61  TEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADL 120
           T+E+VAA+Y+SWLV H KAYNALGE+ERRF+IFKDNLRFIDEHNRE RSY +GL RFADL
Sbjct: 61  TQEQVAALYKSWLVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADL 120

Query: 121 TNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSC 180
           TNEEYR RFLGGRFS KPR SA K+ RYA++LG DLP+HVDWR+KGAV  VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTE 240
           WAFST+AAVEGINQI TGELI+LSEQELVDCD+SYNMGCNGGLMDYAFQFI+DNGGIDTE
Sbjct: 181 WAFSTIAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQL 300
           EDYPYKGRDG CDPNRRNA VVTIDGYEDVPENDE +LKKAVA QPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGRDGVCDPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTG 360
           YQSGVFTGRCGTDLDHGVVAVGYGTE G+DYWIVRNSWGKNWGENGYIRLERNVANITTG
Sbjct: 301 YQSGVFTGRCGTDLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTG 360

Query: 361 KCGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGW 420
           KCGIAIEPSYP+KTGKNPPKPAPSPPSPVKPPTECD+Y+SC EG+TCCCIY+YG+TCFGW
Sbjct: 361 KCGIAIEPSYPVKTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRGIQKLG 480
           GCCPLESATCCDD YSCCP EYP+CDL  GTCR+SKGS IGV LLKR+PAK    +QKLG
Sbjct: 421 GCCPLESATCCDDQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLG 480

Query: 481 KAVFGA 486
           K + G+
Sbjct: 481 KMIIGS 486

[Close]

BLAST of Lcy08g001950 vs. ExPASy TrEMBL
Match: A0A1S3BZW8 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103495259 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 414/485 (85.36%), Postives = 441/485 (90.93%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS    F   AFL LFLC S FSSAS SS+FSIIDENAKHHLGIP+I  S   + QRT
Sbjct: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSD--AHQRT 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVHH KAYNALGEKERRF+IFKDNL FIDEHNRESR+YKVGL RFADLT
Sbjct: 61  DEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLT 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR RFLGGRFSRKP LSA KS RYAAALGDDLPD VDWRKKGAVA+VKDQGQCGSCW
Sbjct: 121 NEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCD+S+NMGCNGGLMDYAFQFIIDNGGIDT+E
Sbjct: 181 AFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDGACDPNR+NAKVVTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVFTGRCGT+LDHGVVAVGYGT+ G DYWIVRNSWGK+WGENGYIRLERNVAN TTGK
Sbjct: 301 QSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIA+EPSYPIK+G NPPKP+ SPPSPV PPTECDEYFSC EGSTCCCIY+YG+TCF WG
Sbjct: 361 CGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRGIQKLGK 480
           CCPLESATCCDDHYSCCPHEYP+CDLEAGTCR SK S +GV+LLKR+PA +T+ IQKLGK
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGK 480

Query: 481 AVFGA 486
              GA
Sbjct: 481 LFVGA 480

[Close]

BLAST of Lcy08g001950 vs. ExPASy TrEMBL
Match: A0A0A0K4N3 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.0e-244
Identity = 411/485 (84.74%), Postives = 442/485 (91.13%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAISP F     AFL LF C S FSSAS SS+FSIIDENAKHHLGIP+I  S   + QR 
Sbjct: 1   MAISPIF----FAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSD--AHQRP 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVHH KAYNA+GEKERRF+IFKDNLRFIDEHNRESR+YKVGL RFADLT
Sbjct: 61  DEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLT 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR RFLGGRFSRKPRLSA KS RYAAALGDDLPD VDWRKKGAVA VKDQGQCGSCW
Sbjct: 121 NEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFS+VAAVEGINQI TGELI LSEQELVDCD+S+NMGCNGGLMDYAFQFII NGGIDTEE
Sbjct: 181 AFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRD ACDPNR+NAKVVTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVFTGRCGTDLDHGVVAVGYGT+ G DYWIVRNSWGK+WGE+GYIRLERNVANITTGK
Sbjct: 301 QSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIA++PSYP K+G NPPKP+ SPPSPVKPPTECDEYFSC+EGSTCCCIY++G+TCF WG
Sbjct: 361 CGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRGIQKLGK 480
           CCPLESATCCDDHYSCCPHEYP+CDLEAGTCR+SK SS+GV+LLKR+PA +T+ +QKLGK
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGK 479

Query: 481 AVFGA 486
              GA
Sbjct: 481 LFVGA 479

[Close]

BLAST of Lcy08g001950 vs. NCBI nr
Match: XP_038889032.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 885.9 bits (2288), Expect = 1.5e-253
Identity = 423/486 (87.04%), Postives = 452/486 (93.00%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADS-QVGSPQR 60
           MAIS  FSF  +AFL LFLCFS FSSAS SS+FSIIDENAKHH+GIPDIADS  + SPQR
Sbjct: 1   MAISSTFSFLTLAFLALFLCFSPFSSASHSSTFSIIDENAKHHMGIPDIADSDHLRSPQR 60

Query: 61  TEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADL 120
           T++EVAA+YESWLVHHRKAYNALGEKERRF+IFKDNLRFIDEHNRESR+YKVGL RFADL
Sbjct: 61  TDDEVAALYESWLVHHRKAYNALGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL 120

Query: 121 TNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSC 180
           TNEEYR RFLGGRF  KPRLSA KS RYAAALGDDLPDHVDWRKKGAVA VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFPGKPRLSAAKSGRYAAALGDDLPDHVDWRKKGAVAAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTE 240
           WAFSTVAAVEGINQI TGELI+LSEQELVDCD+SYNMGCNGGLMDYAFQFIIDNGGIDTE
Sbjct: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQL 300
           EDYPYKG D ACDPNR+NAKVVTIDG+EDVPENDE SLKKAVA QPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGHDAACDPNRKNAKVVTIDGFEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTG 360
           YQSGVFTGRCGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGE+GYIRLERNVANITTG
Sbjct: 301 YQSGVFTGRCGTNLDHGVVAVGYGTENGTDYWIVRNSWGKNWGESGYIRLERNVANITTG 360

Query: 361 KCGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGW 420
           KCGIA++PSYPIKTG NPPKPA SPPSPVKPPTECD+Y+SC+EG+TCCCIY+YG+TCFGW
Sbjct: 361 KCGIAVQPSYPIKTGSNPPKPAASPPSPVKPPTECDDYYSCEEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRGIQKLG 480
           GCCPLESATCCDDHYSCCPHEYPICDLEAGTC + K S++GV LLKR+PAKRT+GIQK G
Sbjct: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTCLIDKDSTMGVKLLKRLPAKRTKGIQKFG 480

Query: 481 KAVFGA 486
           +   GA
Sbjct: 481 ELFVGA 486

[Close]

BLAST of Lcy08g001950 vs. NCBI nr
Match: XP_022988755.1 (low-temperature-induced cysteine proteinase-like [Cucurbita maxima])

HSP 1 Score: 864.8 bits (2233), Expect = 3.6e-247
Identity = 420/486 (86.42%), Postives = 439/486 (90.33%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS  FSFS+VAFL LFLCFSS S ASDSSSFSIIDENAKHHL      D Q+GSP R+
Sbjct: 1   MAISTTFSFSIVAFLALFLCFSSSSLASDSSSFSIIDENAKHHL------DVQIGSPHRS 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVH+ KAYNALGEKERRF+IFKDNLRFIDEHNRESRSYKVGLNRFADL+
Sbjct: 61  DEEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLS 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR  FL GR SRK RLSA KSRRYA  LGDDLPDHVDWRKKGAV DVKDQGQCGSCW
Sbjct: 121 NEEYRAMFLSGRLSRKNRLSATKSRRYAITLGDDLPDHVDWRKKGAVTDVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE
Sbjct: 181 AFSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDG CDPNRRN+K VTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGTCDPNRRNSKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVF G CGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGENGYI+LERNV NITTGK
Sbjct: 301 QSGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIAIE SYP KTGKNPPKPAPSPPSPV PP +CDEYFSCQEG+TCCCIY+YG+TCFGWG
Sbjct: 361 CGIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRG-IQKLG 480
           CCPLESATCCDDHYSCCPHEYPICDL+AGTC  SKGS+ GV LLKRVPAK  RG +QKLG
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTTGVKLLKRVPAKYNRGCVQKLG 480

Query: 481 KAVFGA 486
           K   GA
Sbjct: 481 KMSIGA 480

[Close]

BLAST of Lcy08g001950 vs. NCBI nr
Match: KAG7022467.1 (putative cysteine protease RD21B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 864.4 bits (2232), Expect = 4.7e-247
Identity = 421/486 (86.63%), Postives = 441/486 (90.74%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS  FSFS+V FLPLFLCFSS SSASDSS+FSIIDENAKHHL      D Q+GS QR+
Sbjct: 1   MAISTTFSFSIVVFLPLFLCFSSSSSASDSSTFSIIDENAKHHL------DIQIGSSQRS 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVH+ KAYNALGEKERRF+IFKDNLRFIDEHNRESRSYKVGLNRFADL+
Sbjct: 61  DEEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLS 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR  FL GR SRK RLSA KSRRYA ALGDDLPDHVDWRKKGAVADVKDQGQCGSCW
Sbjct: 121 NEEYRAMFLSGRLSRKNRLSATKSRRYAIALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE
Sbjct: 181 AFSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDG CDP R+NAK VTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGTCDPIRKNAKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVF G CGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGENGYI+LERNV NITTGK
Sbjct: 301 QSGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIAIE SYP KTGKNPPKPAPSPPSPV PP +CDEYFSCQEG+TCCCIY+YG+TCFGWG
Sbjct: 361 CGIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRG-IQKLG 480
           CCPLESATCCDDHYSCCPHEYPICDL+AGTC  SKGS+ GV+LLKRVPAK  RG  QKLG
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTSGVELLKRVPAKYNRGCAQKLG 480

Query: 481 KAVFGA 486
           K   GA
Sbjct: 481 KMSTGA 480

[Close]

BLAST of Lcy08g001950 vs. NCBI nr
Match: XP_023531081.1 (low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.4 bits (2227), Expect = 1.8e-246
Identity = 419/486 (86.21%), Postives = 440/486 (90.53%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS  FSFS+V FL LFLCFSS SSASDSSSFSIIDENAKHHL      D Q+GS QR+
Sbjct: 1   MAISTTFSFSIVVFLALFLCFSSSSSASDSSSFSIIDENAKHHL------DIQIGSSQRS 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVH+ KAYNALGEKERRF+IFKDNLRFIDEHNRESRSYK+GLNRFADL+
Sbjct: 61  DEEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKIGLNRFADLS 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR  FL GR SRK RLSA KSRRYA ALGDDLPDHVDWRKKGAV DVKDQGQCGSCW
Sbjct: 121 NEEYRAMFLSGRLSRKNRLSATKSRRYAIALGDDLPDHVDWRKKGAVTDVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE
Sbjct: 181 AFSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDG CDP R+NAK VTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGTCDPIRKNAKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVF G CGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGENGYI+LERNV NITTGK
Sbjct: 301 QSGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIAIE SYP KTGKNPPKPAPSPPSPV PP +CDEYFSCQEG+TCCCIY+YG+TCFGWG
Sbjct: 361 CGIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRG-IQKLG 480
           CCPLESATCCDDHYSCCPHEYPICDL+AGTC  SKGS+ GV+LLKRVPAK  RG +QKLG
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTTGVELLKRVPAKYNRGCVQKLG 480

Query: 481 KAVFGA 486
           K   GA
Sbjct: 481 KMSTGA 480

[Close]

BLAST of Lcy08g001950 vs. NCBI nr
Match: XP_022927732.1 (low-temperature-induced cysteine proteinase-like [Cucurbita moschata])

HSP 1 Score: 861.7 bits (2225), Expect = 3.1e-246
Identity = 419/486 (86.21%), Postives = 439/486 (90.33%), Query Frame = 0


Query: 1   MAISPAFSFSVVAFLPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRT 60
           MAIS  F FS+V FLPLFLCFSS SSASDSSSFSIIDENA+HHL      D Q+GS QR+
Sbjct: 1   MAISTTFCFSIVVFLPLFLCFSSSSSASDSSSFSIIDENARHHL------DIQIGSSQRS 60

Query: 61  EEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLT 120
           +EEVAA+YESWLVH+ KAYNALGEKERRF+IFKDNLRFIDEHNRESRSYKVGLNRFADL+
Sbjct: 61  DEEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLS 120

Query: 121 NEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCW 180
           NEEYR  FL GR SRK RLSA KSRRYA ALGDDLPDHVDWRKKGAV DVKDQGQCGSCW
Sbjct: 121 NEEYRAMFLSGRLSRKNRLSATKSRRYAIALGDDLPDHVDWRKKGAVTDVKDQGQCGSCW 180

Query: 181 AFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240
           AFSTVAAVEGINQI TGELI+LSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE
Sbjct: 181 AFSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEE 240

Query: 241 DYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLY 300
           DYPYKGRDG CDP R+NAK VTIDGYEDVPENDE SLKKAVA QPVSVAIEAGGRAFQLY
Sbjct: 241 DYPYKGRDGTCDPIRKNAKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 300

Query: 301 QSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGK 360
           QSGVF G CGT+LDHGVVAVGYGTE G DYWIVRNSWGKNWGENGYI+LERNV NITTGK
Sbjct: 301 QSGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGK 360

Query: 361 CGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWG 420
           CGIAIE SYP KTGKNPPKPAPSPPSPV PP +CDEYFSCQEG+TCCCIY+YG+TCFGWG
Sbjct: 361 CGIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWG 420

Query: 421 CCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRTRG-IQKLG 480
           CCPLESATCCDDHYSCCPHEYPICDL+AGTC  SKGS+ GV+LLKRVPAK  RG  QKLG
Sbjct: 421 CCPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTSGVELLKRVPAKYNRGCAQKLG 480

Query: 481 KAVFGA 486
           K   GA
Sbjct: 481 KMSIGA 480

[Close]

BLAST of Lcy08g001950 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 641.7 bits (1654), Expect = 4.6e-184
Identity = 309/455 (67.91%), Postives = 363/455 (79.78%), Query Frame = 0


Query: 17  LFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVHHR 76
           LFL   + SSA D    SII  + KH +       S  G   R+E EV +IYE+WLV H 
Sbjct: 11  LFLAMVAVSSAVD---MSIISYDEKHGV-------STTGG--RSEAEVMSIYEAWLVKHG 70

Query: 77  KA--YNALGEKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLTNEEYRTRFLGGRFS 136
           KA   N+L EK+RRF+IFKDNLRF+DEHN ++ SY++GL RFADLTN+EYR+++LG +  
Sbjct: 71  KAQSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKME 130

Query: 137 RKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTVAAVEGINQI 196
           +K       S RY A +GD+LP+ +DWRKKGAVA+VKDQG CGSCWAFST+ AVEGINQI
Sbjct: 131 KKGERRT--SLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQI 190

Query: 197 ATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYKGRDGACDPN 256
            TG+LITLSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDT++DYPYKG DG CD  
Sbjct: 191 VTGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQI 250

Query: 257 RRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLD 316
           R+NAKVVTID YEDVP   E SLKKAVA QP+S+AIEAGGRAFQLY SG+F G CGT LD
Sbjct: 251 RKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLD 310

Query: 317 HGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPIKTG 376
           HGVVAVGYGTE G DYWIVRNSWGK+WGE+GY+R+ RN+A+ ++GKCGIAIEPSYPIK G
Sbjct: 311 HGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIAS-SSGKCGIAIEPSYPIKNG 370

Query: 377 KNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWGCCPLESATCCDDHY 436
           +NPP P PSPPSP+KPPT+CD Y++C E +TCCC++EYG  CF WGCCPLE+ATCCDD+Y
Sbjct: 371 ENPPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNY 430

Query: 437 SCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPA 470
           SCCPHEYP+CDL+ GTC LSK S   V  LKR PA
Sbjct: 431 SCCPHEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

[Close]

BLAST of Lcy08g001950 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 628.2 bits (1619), Expect = 5.3e-180
Identity = 308/458 (67.25%), Postives = 350/458 (76.42%), Query Frame = 0


Query: 17  LFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVHHR 76
           L L     S A D    SII  +  HH+              R++ EV  IYE+W+V H 
Sbjct: 11  LLLAMIGVSYAMD---MSIISYDENHHI---------TTETSRSDSEVERIYEAWMVEHG 70

Query: 77  KA---YNALG-EKERRFQIFKDNLRFIDEHNRESRSYKVGLNRFADLTNEEYRTRFLGGR 136
           K     N LG EK++RF+IFKDNLRFIDEHN ++ SYK+GL RFADLTNEEYR+ +LG  
Sbjct: 71  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGA- 130

Query: 137 FSRKPRLSAVK-SRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTVAAVEGI 196
              KP    +K S RY A +GD LPD VDWRK+GAVADVKDQG CGSCWAFST+ AVEGI
Sbjct: 131 ---KPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGI 190

Query: 197 NQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYKGRDGAC 256
           N+I TG+LI+LSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDTE DYPYK  DG C
Sbjct: 191 NKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRC 250

Query: 257 DPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVFTGRCGT 316
           D NR+NAKVVTID YEDVPEN E SLKKA+A QP+SVAIEAGGRAFQLY SGVF G CGT
Sbjct: 251 DQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGT 310

Query: 317 DLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPI 376
           +LDHGVVAVGYGTE G DYWIVRNSWG  WGE+GYI++ RN+    TGKCGIA+E SYPI
Sbjct: 311 ELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEASYPI 370

Query: 377 KTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNTCFGWGCCPLESATCCD 436
           K G+NPP P PSPPSP+KPPT CD+YFSC E +TCCC+Y+YG  CFGWGCCPLE+ATCCD
Sbjct: 371 KKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCD 430

Query: 437 DHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPA 470
           D+ SCCPHEYP+CD+  GTC +SK S   V  LKR PA
Sbjct: 431 DNSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPA 451

[Close]

BLAST of Lcy08g001950 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 554.7 bits (1428), Expect = 7.5e-158
Identity = 267/417 (64.03%), Postives = 314/417 (75.30%), Query Frame = 0


Query: 59  RTEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNR-ESRSYKVGLNRFA 118
           R E E   +YE WLV +RK YN LGEKERRF+IFKDNL+F++EH+   +R+Y+VGL RFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 119 DLTNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQGQCG 178
           DLTN+E+R  +L  +  R      VK  +Y   +GD LPD +DWR KGAV  VKDQG CG
Sbjct: 94  DLTNDEFRAIYLRSKMER--TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCG 153

Query: 179 SCWAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGID 238
           SCWAFS + AVEGINQI TGELI+LSEQELVDCD SYN GC GGLMDYAF+FII+NGGID
Sbjct: 154 SCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGID 213

Query: 239 TEEDYPYKGRD-GACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRA 298
           TEEDYPY   D   C+ +++N +VVTIDGYEDVP+NDE SLKKA+A QP+SVAIEAGGRA
Sbjct: 214 TEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRA 273

Query: 299 FQLYQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANI 358
           FQLY SGVFTG CGT LDHGVVAVGYG+EGG DYWIVRNSWG NWGE+GY +LERN+   
Sbjct: 274 FQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE- 333

Query: 359 TTGKCGIAIEPSYPIK-TGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGNT 418
           ++GKCG+A+  SYP K +G NPPKP      P   P  CD+  +C   STCCC+YEY   
Sbjct: 334 SSGKCGVAMMASYPTKSSGSNPPKP------PAPSPVVCDKSNTCPAKSTCCCLYEYNGK 393

Query: 419 CFGWGCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGSSIGVDLLKRVPAKRT 473
           C+ WGCCP ESATCCDD  SCCP  YP+CDL+A TCR+   S + +  L R PA  T
Sbjct: 394 CYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIAT 441

[Close]

BLAST of Lcy08g001950 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 436.8 bits (1122), Expect = 2.3e-122
Identity = 209/403 (51.86%), Postives = 271/403 (67.25%), Query Frame = 0


Query: 56  SPQRTEEEVAAIYESWLVHHRKAYNALGEKERRFQIFKDNLRFIDEHNR-ESRSYKVGLN 115
           S   + ++++ +++ W   H K Y +  E+++R QIFKDN  F+ +HN   + +Y + LN
Sbjct: 20  SSSSSSDDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLN 79

Query: 116 RFADLTNEEYRTRFLGGRFSRKPRLSAVKSRRYAAALGDDLPDHVDWRKKGAVADVKDQG 175
            FADLT+ E++   LG   S    + A K +    ++   +PD VDWRKKGAV +VKDQG
Sbjct: 80  AFADLTHHEFKASRLGLSVSAPSVIMASKGQSLGGSV--KVPDSVDWRKKGAVTNVKDQG 139

Query: 176 QCGSCWAFSTVAAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNG 235
            CG+CW+FS   A+EGINQI TG+LI+LSEQEL+DCD+SYN GCNGGLMDYAF+F+I N 
Sbjct: 140 SCGACWSFSATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVIKNH 199

Query: 236 GIDTEEDYPYKGRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGG 295
           GIDTE+DYPY+ RDG C  ++   KVVTID Y  V  NDE +L +AVA QPVSV I    
Sbjct: 200 GIDTEKDYPYQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSE 259

Query: 296 RAFQLYQSGVFTGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVA 355
           RAFQLY SG+F+G C T LDH V+ VGYG++ GVDYWIV+NSWGK+WG +G++ ++RN  
Sbjct: 260 RAFQLYSSGIFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTE 319

Query: 356 NITTGKCGIAIEPSYPIKTGKNPPKPAPSPPSPVKPPTECDEYFSCQEGSTCCCIYEYGN 415
           N + G CGI +  SYPIKT  NP  P PSPP     PT+C+ +  C  G TCCC  E   
Sbjct: 320 N-SDGVCGINMLASYPIKTHPNP--PPPSPPG----PTKCNLFTYCSSGETCCCARELFG 379

Query: 416 TCFGWGCCPLESATCCDDHYSCCPHEYPICDLEAGTCRLSKGS 458
            CF W CC +ESA CC D   CCPH+YP+CD     C    G+
Sbjct: 380 LCFSWKCCEIESAVCCKDGRHCCPHDYPVCDTTRSLCLKKTGN 413

[Close]

BLAST of Lcy08g001950 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 429.9 bits (1104), Expect = 2.8e-120
Identity = 220/372 (59.14%), Postives = 274/372 (73.66%), Query Frame = 0


Query: 15  LPLFLCFSSFSSASDSSSFSIIDENAKHHLGIPDIADSQVGSPQRTEEEVAAIYESWLVH 74
           L L L +   S AS     SII++    HL +P           RT+EEV +IY  W   
Sbjct: 8   LSLLLLYVVVSLASGDE--SIIND----HLQLPS------DGKWRTDEEVRSIYLQWSAE 67

Query: 75  HRKAYN----ALGEKERRFQIFKDNLRFIDEHNRESR--SYKVGLNRFADLTNEEYRTRF 134
           H K  N     + ++++RF IFKDNLRFID HN  ++  +YK+GL +F DLTN+EYR  +
Sbjct: 68  HGKTNNNNNGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLY 127

Query: 135 LGGRFSRKPRLSAVK--SRRYAAAL-GDDLPDHVDWRKKGAVADVKDQGQCGSCWAFSTV 194
           LG R     R++  K  +++Y+AA+ G ++P+ VDWR+KGAV  +KDQG CGSCWAFST 
Sbjct: 128 LGARTEPARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTT 187

Query: 195 AAVEGINQIATGELITLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDYPYK 254
           AAVEGIN+I TGELI+LSEQELVDCD+SYN GCNGGLMDYAFQFI+ NGG++TE+DYPY+
Sbjct: 188 AAVEGINKIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYR 247

Query: 255 GRDGACDPNRRNAKVVTIDGYEDVPENDEISLKKAVAGQPVSVAIEAGGRAFQLYQSGVF 314
           G  G C+   +N++VV+IDGYEDVP  DE +LKKA++ QPVSVAIEAGGR FQ YQSG+F
Sbjct: 248 GFGGKCNSFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIF 307

Query: 315 TGRCGTDLDHGVVAVGYGTEGGVDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAI 374
           TG CGT+LDH VVAVGYG+E GVDYWIVRNSWG  WGE GYIR+ERN+A   +GKCGIA+
Sbjct: 308 TGSCGTNLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAV 367

Query: 375 EPSYPIKTGKNP 378
           E SYP+K   NP
Sbjct: 368 EASYPVKYSPNP 367

The following BLAST results are available for this feature:

BLAST of Lcy08g001950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	6.5e-183	67.91	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	7.5e-179	67.25	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25776	5.9e-176	68.09	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
Q9LT78	1.1e-156	64.03	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25777	2.1e-152	59.35	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]

BLAST of Lcy08g001950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKG6	1.8e-247	86.42	low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1EI11	1.5e-246	86.21	low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1D149	2.1e-245	83.95	low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 ...	[more]
A0A1S3BZW8	1.1e-244	85.36	low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0K4N3	4.0e-244	84.74	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1	[more]

BLAST of Lcy08g001950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889032.1	1.5e-253	87.04	low-temperature-induced cysteine proteinase-like [Benincasa hispida]	[more]
XP_022988755.1	3.6e-247	86.42	low-temperature-induced cysteine proteinase-like [Cucurbita maxima]	[more]
KAG7022467.1	4.7e-247	86.63	putative cysteine protease RD21B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023531081.1	1.8e-246	86.21	low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927732.1	3.1e-246	86.21	low-temperature-induced cysteine proteinase-like [Cucurbita moschata]	[more]

BLAST of Lcy08g001950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	4.6e-184	67.91	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	5.3e-180	67.25	Granulin repeat cysteine protease family protein	[more]
AT3G19390.1	7.5e-158	64.03	Granulin repeat cysteine protease family protein	[more]
AT1G09850.1	2.3e-122	51.86	xylem bark cysteine peptidase 3	[more]
AT4G36880.1	2.8e-120	59.14	cysteine proteinase1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 315..325 score: 58.59 coord: 173..188 score: 66.81 coord: 330..336 score: 73.06
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 155..371 e-value: 1.4E-127 score: 439.8
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 155..371 e-value: 1.3E-84 score: 283.7
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 394..451 e-value: 4.4E-25 score: 99.3
IPR000118	Granulin	PFAM	PF00396	Granulin	coord: 405..451 e-value: 2.5E-10 score: 40.6
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 68..124 e-value: 1.9E-25 score: 100.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 68..124 e-value: 1.5E-17 score: 63.8
IPR037277	Granulin superfamily	GENE3D	2.10.25.160	Granulin	coord: 391..453 e-value: 3.6E-13 score: 51.6
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 52..373 e-value: 6.8E-121 score: 405.7
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 48..400
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 48..400
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 391..425
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 330..349
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 173..184
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 313..323
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 156..370 e-value: 1.3544E-111 score: 326.888
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 60..371

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy08g001950.1	Lcy08g001950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity