Lcy08g001470 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g001470
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAmino acid permease family protein
LocationChr08: 2056005 .. 2058832 (+)
RNA-Seq ExpressionLcy08g001470
SyntenyLcy08g001470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGGCTCTAGTAGCACATGCCACGCCACGAGGAAGATGACAAAGTCAACCATCCCCGGCCATAATCAACAATTTTCTGAGAGAGATTCAATACAGTGTCCCAACCCCAAAGATTCTTCTTCTTCTTCTTCTCTCTCGCTCATCCTCTTCTGGGCAATTCAAACGATTCCCATCCATCAAAACACAGCCCAGATTTAGCTTCACTCGTTTGAAATTTCCAGCAGATCTTGTTCATTCCTTGAAATATCCCAATTCCACTTGATTTTCAAGGTGTTTAAGAACAACCCTTCTTCTCGTTCCTGTCCATGGGCGAAGAGAGAGGGTCCGATGCTAAAATTGCTCATAAACTAACGATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCCTTTGGAGTGGAGGATTCGGTGAGCTGTGGCGGCGGCCCTCTTCTGGCTTTGTTGGGTTTCGTAGTGTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACGAGTTTCCCCCAGAATGGTGGATATGTGGTATGGATTTCGGCCGCTTTTGGCCCTTTTTGGGGTTTTCAAGAGGGGTTTTGGAAATGGATCAGTGGGGTAATGGATAATGCTCTGTATCCTGTTCTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATTTGTTAGCTCGAATTCCAGCTCTGTTAGGGATTACAGCCTCTTTAACTTACTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTTTCCATTTGCGGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCCAAAAAGTGGCTAGTTCTGGATTATAGCAAGGTGAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTCTAGCAGGGGAGATTGAAAATCCTAGCAAAACTTTCCCCAAGGCAATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCCTTGGAGTCAGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGCTGGGGCCTTGATTGGTGGGGTGTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCCATGTCTAACATGGGGTTGTTTGAAGCTGAAATGAGCAGTGATGCATACCAACTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGGTTGGTGGTTTTCAGTTTCCTTTTTCATTGTTGTATTTTGGTAGCAAATTTGAGTAACAACAGTCTCTCCTGATGTTCCCACCGATGAGTTTTGTAGCTCAAATCGGTAGTAGGGATTCAAAATAGCAATCCTTCGAGTGAGAGGTCAAAGGTTTAAATCACTGGGGAGTAAAATCAAAACTTGTGAGTTCAATTGGGTCGGGTTAACAATATGTTTTAGTGGTAAGTTGACATTGACACGAACCATCACCATAAAAAGGATGATAGGATGACAAAGATGGATGAGATTGATAGCCTGAAAGTATCATAGAATCCCTAGAATTATAAGACTCCTTGGGGGACTTTTCGCCTCCAAGATGAGTTTGTATAGCTTAAAATTATAAACTAGAGCTTACATTTGTTAGCTGGATTTACTTATGATAAGATACATGTTTGTTTCATAAACTCAGGTTACATAAATTCAAACTCTTGAAATATATCTCTCTTGCCACACCGCCCTAAACACGAGAATCAAAGTAGGTACCTTTCTACTCTCTAGGTCTATATCCAAGTATGGGGCTATACAAACTCAAACTTTTCAACCTTCAACATGTTAATTAAACAACCATAAGCCAAACATTTCCTAAACTCCTCTCCTATGTCCATATCCTAGCGTGCTTCCTCCTTGAACTCTTCCAGCATTTTGATTCATTTGATTTACTGGCATCATGTGGATACAAAAACTGCACTTATTGAACTACTGTCTATTTCACTTTGCAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCAGCCTTTGGGGTTATCTTCGTCTCATGGTTGAGTTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTTAACAGACCTTACAGAGTTCCCTTGCAAACATTTGGTGCCACAATGCTTTGCTTGCCACCTACAGCCTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCTCAAACATTCTTAATCAGTGGGATTATAATTGTTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAGGCAAAGAACAGAAGATGGGCCAAATTCATTTCAGTACAGCCAGCAGCTACTCTTTCTGATGCGGAAGATAGGTTGGCCGAGTCGCAACCGGTGCAAGAAGTTTCTGATGAGGCTGCGGTTCGGCTACTTTCAGAATCTTCTCCATCTTCGATTATAGCACAGCAATAACATCCGGATGCAGTCGGAAGCTTTACGAATAGGGAAATTTCACTTCTGCAATGTTCTGAGAAGATGGTGTGTTTGAGTAACAAAATATTGAAGTTACACAGAACAATACATTGTTATGCAAACATATTTATGAACAACTTGTTTTATATTCTTTTGAAAGGTATTGTACTTCTACTGATATGCTGCCTGTGGATGTAATGCAAAAGGGTTAATTTTGACCTGTGTTTTTACCAAGCTCAAGGTGCACTAAAGTGTAATAGCCCTTTGAGACTCAAGTGCAGACATAAAGTTAAGTTTTTTTTTT

mRNA sequence

TAGGCTCTAGTAGCACATGCCACGCCACGAGGAAGATGACAAAGTCAACCATCCCCGGCCATAATCAACAATTTTCTGAGAGAGATTCAATACAGTGTCCCAACCCCAAAGATTCTTCTTCTTCTTCTTCTCTCTCGCTCATCCTCTTCTGGGCAATTCAAACGATTCCCATCCATCAAAACACAGCCCAGATTTAGCTTCACTCGTTTGAAATTTCCAGCAGATCTTGTTCATTCCTTGAAATATCCCAATTCCACTTGATTTTCAAGGTGTTTAAGAACAACCCTTCTTCTCGTTCCTGTCCATGGGCGAAGAGAGAGGGTCCGATGCTAAAATTGCTCATAAACTAACGATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCCTTTGGAGTGGAGGATTCGGTGAGCTGTGGCGGCGGCCCTCTTCTGGCTTTGTTGGGTTTCGTAGTGTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACGAGTTTCCCCCAGAATGGTGGATATGTGGTATGGATTTCGGCCGCTTTTGGCCCTTTTTGGGGTTTTCAAGAGGGGTTTTGGAAATGGATCAGTGGGGTAATGGATAATGCTCTGTATCCTGTTCTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATTTGTTAGCTCGAATTCCAGCTCTGTTAGGGATTACAGCCTCTTTAACTTACTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTTTCCATTTGCGGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCCAAAAAGTGGCTAGTTCTGGATTATAGCAAGGTGAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTCTAGCAGGGGAGATTGAAAATCCTAGCAAAACTTTCCCCAAGGCAATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCCTTGGAGTCAGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGCTGGGGCCTTGATTGGTGGGGTGTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCCATGTCTAACATGGGGTTGTTTGAAGCTGAAATGAGCAGTGATGCATACCAACTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCAGCCTTTGGGGTTATCTTCGTCTCATGGTTGAGTTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTTAACAGACCTTACAGAGTTCCCTTGCAAACATTTGGTGCCACAATGCTTTGCTTGCCACCTACAGCCTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCTCAAACATTCTTAATCAGTGGGATTATAATTGTTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAGGCAAAGAACAGAAGATGGGCCAAATTCATTTCAGTACAGCCAGCAGCTACTCTTTCTGATGCGGAAGATAGGTTGGCCGAGTCGCAACCGGTGCAAGAAGTTTCTGATGAGGCTGCGGTTCGGCTACTTTCAGAATCTTCTCCATCTTCGATTATAGCACAGCAATAACATCCGGATGCAGTCGGAAGCTTTACGAATAGGGAAATTTCACTTCTGCAATGTTCTGAGAAGATGGTGTGTTTGAGTAACAAAATATTGAAGTTACACAGAACAATACATTGTTATGCAAACATATTTATGAACAACTTGTTTTATATTCTTTTGAAAGGTATTGTACTTCTACTGATATGCTGCCTGTGGATGTAATGCAAAAGGGTTAATTTTGACCTGTGTTTTTACCAAGCTCAAGGTGCACTAAAGTGTAATAGCCCTTTGAGACTCAAGTGCAGACATAAAGTTAAGTTTTTTTTTT

Coding sequence (CDS)

ATGGGCGAAGAGAGAGGGTCCGATGCTAAAATTGCTCATAAACTAACGATTCTTCCTCTAATCGCTTTGATTTTCTACGACGTTTCTGGAGGACCCTTTGGAGTGGAGGATTCGGTGAGCTGTGGCGGCGGCCCTCTTCTGGCTTTGTTGGGTTTCGTAGTGTTTCCGTTTATTTGGAGTATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACGAGTTTCCCCCAGAATGGTGGATATGTGGTATGGATTTCGGCCGCTTTTGGCCCTTTTTGGGGTTTTCAAGAGGGGTTTTGGAAATGGATCAGTGGGGTAATGGATAATGCTCTGTATCCTGTTCTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAACCATTTGTTAGCTCGAATTCCAGCTCTGTTAGGGATTACAGCCTCTTTAACTTACTTGAACTATCGTGGTCTGCACATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTTTCCATTTGCGGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCCAAAAAGTGGCTAGTTCTGGATTATAGCAAGGTGAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATCTGAACTATTGGGATAAAGCAAGTACTCTAGCAGGGGAGATTGAAAATCCTAGCAAAACTTTCCCCAAGGCAATGTTTGGAGCTGTGGTTTTGGTGGTTTCTTCTTATTTGATTCCTCTTCTGGCTGGGACTGGTGCCTTGGAGTCAGATTCAAGTGAATGGAGTGATGGGTATTTTGCAGAGGCTGGGGCCTTGATTGGTGGGGTGTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCCATGTCTAACATGGGGTTGTTTGAAGCTGAAATGAGCAGTGATGCATACCAACTGCTTGGGATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCAGCCTTTGGGGTTATCTTCGTCTCATGGTTGAGTTTCCAAGAAATACTTGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTGTAAAACTAAGAATAAAGAAGCCAGACCTTAACAGACCTTACAGAGTTCCCTTGCAAACATTTGGTGCCACAATGCTTTGCTTGCCACCTACAGCCTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCTCAAACATTCTTAATCAGTGGGATTATAATTGTTGTGGGGTTTCTTCTATACCCTACTCTTTTGCAGGCAAAGAACAGAAGATGGGCCAAATTCATTTCAGTACAGCCAGCAGCTACTCTTTCTGATGCGGAAGATAGGTTGGCCGAGTCGCAACCGGTGCAAGAAGTTTCTGATGAGGCTGCGGTTCGGCTACTTTCAGAATCTTCTCCATCTTCGATTATAGCACAGCAATAA

Protein sequence

MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQAKNRRWAKFISVQPAATLSDAEDRLAESQPVQEVSDEAAVRLLSESSPSSIIAQQ
Homology
BLAST of Lcy08g001470 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.0e-204
Identity = 355/477 (74.42%), Postives = 410/477 (85.95%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           + +E  S  K + KLT+LPL+ LIFY+VSGGPFGVEDSV  GGGPLLALLGF++FP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKW SGVMDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
            SFPV +H+ AR+PALL IT SLTYLNYRGLHIVGFSAVVLAVFSL PF VM +L++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPK+WL +D  K+NWRGYFN+MFWNLNYWDKASTLAGE++ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESDSS-EWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S +S EWSDGYFAE G LIGGVWLK WIQAAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGM 360
           A+QLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIF+SW+SFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIII 420
           LLE AAFVKLRIKKPDL+RPYRVPL TFG +MLCLPP+ L++LVM LA+ +TFLISG+II
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 VVGFLLYPTLLQAKNRRWAKFI--SVQPAATLSDAEDRLAESQPVQEVSDEAAVRLL 475
           V+GF LYP L   K ++WA+FI    +P + +S      +ESQ  +E  DE+A  LL
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEETRPVSGVS------SESQLDEEHGDESAASLL 478

BLAST of Lcy08g001470 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.6e-152
Identity = 251/427 (58.78%), Postives = 333/427 (77.99%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGFV+FPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW+SGV+DNALYPVLFLDYLK   P     L R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PFAVM ++SIP++ P +WLV+D   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGE+ENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++    +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ +SWLSFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PPT L+  V+ L+S +   +S +++++GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 NRRWAKF 441
            +RW KF
Sbjct: 468 RKRWVKF 473

BLAST of Lcy08g001470 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.8e-149
Identity = 251/429 (58.51%), Postives = 329/429 (76.69%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATS 73
           K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF+VFPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW+SGV+DNALYP+LFLDYLK   P+    + R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLD--YS 193
            A+L +T +LTYLNYRGL IVG +AV+L VFS+ PF VM+ +SIP+++P +WLV+     
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  STL GE+ENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGM 313
           +  D   W+DGYFA+ G +IGGVWL WWIQAAAA SNMG+F AEMSSD++QLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRI 373
           +P VFA RS+Y TP   IL SA GVI +SWLSFQEI+   N LY  GM+LE   FV+LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQ 433
           K P  +RP+++P+   G+ ++C+PPT L+ ++M   + +  L+S   IV+G +L P L Q
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

Query: 434 AKNRRWAKF 441
            + + W KF
Sbjct: 472 VEKKGWLKF 479

BLAST of Lcy08g001470 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.3e-146
Identity = 252/444 (56.76%), Postives = 332/444 (74.77%), Query Frame = 0

Query: 2   GEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSI 61
           G+  G     A  ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GF+V P IWSI
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSI 129

Query: 62  PEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKR 121
           PEAL+TAEL   FP+NGGYVVW+++A GP+WGFQ+G+ KW+SGV+DNALYPVLFLDYLK 
Sbjct: 130 PEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKS 189

Query: 122 SFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIR 181
             P       R  A++G+TA LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++R
Sbjct: 190 GVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLR 249

Query: 182 PKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSY 241
           P +WLV+D   V+W  Y N++FWNLNYWD  STLAGE++NP KT PKA+F AV+ VV +Y
Sbjct: 250 PARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAY 309

Query: 242 LIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAY 301
           L PLLAGTGA+  D  +W+DGYFA+   L+GG WL WW+Q+AAA+SNMG+F AEMSSD+Y
Sbjct: 310 LYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSY 369

Query: 302 QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLL 361
           QLLGM+E GM+PS FA+RS+YGTP   IL SA GV+ +S +SFQEI+   NFLY  GMLL
Sbjct: 370 QLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLL 429

Query: 362 ELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVV 421
           E  AF+  R+++PD  RPYRVPL T G   + +PPTAL+ +V+ L++ +  ++S   + +
Sbjct: 430 EFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAM 489

Query: 422 GFLLYPTLLQAKNRRWAKFISVQP 446
           G +L P L   + +RW +F SV P
Sbjct: 490 GLVLQPALRFVEKKRWLRF-SVNP 511

BLAST of Lcy08g001470 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.3e-146
Identity = 252/444 (56.76%), Postives = 332/444 (74.77%), Query Frame = 0

Query: 2   GEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSI 61
           G+  G     A  ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GF+V P IWSI
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSI 129

Query: 62  PEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKR 121
           PEAL+TAEL   FP+NGGYVVW+++A GP+WGFQ+G+ KW+SGV+DNALYPVLFLDYLK 
Sbjct: 130 PEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKS 189

Query: 122 SFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIR 181
             P       R  A++G+TA LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++R
Sbjct: 190 GVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLR 249

Query: 182 PKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSY 241
           P +WLV+D   V+W  Y N++FWNLNYWD  STLAGE++NP KT PKA+F AV+ VV +Y
Sbjct: 250 PARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAY 309

Query: 242 LIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAY 301
           L PLLAGTGA+  D  +W+DGYFA+   L+GG WL WW+Q+AAA+SNMG+F AEMSSD+Y
Sbjct: 310 LYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSY 369

Query: 302 QLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLL 361
           QLLGM+E GM+PS FA+RS+YGTP   IL SA GV+ +S +SFQEI+   NFLY  GMLL
Sbjct: 370 QLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLL 429

Query: 362 ELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVV 421
           E  AF+  R+++PD  RPYRVPL T G   + +PPTAL+ +V+ L++ +  ++S   + +
Sbjct: 430 EFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAM 489

Query: 422 GFLLYPTLLQAKNRRWAKFISVQP 446
           G +L P L   + +RW +F SV P
Sbjct: 490 GLVLQPALRFVEKKRWLRF-SVNP 511

BLAST of Lcy08g001470 vs. ExPASy TrEMBL
Match: A0A5D3C4H2 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001140 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.9e-244
Identity = 431/486 (88.68%), Postives = 452/486 (93.00%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKW SG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGIII 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDAEDRLAESQPVQEVSDEAAVRLLSESSP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSD EDRL E QP QEV +EA VRLLSESS 
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SSIIAQ 486
           S+I  Q
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. ExPASy TrEMBL
Match: A0A1S3C014 (probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495296 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.5e-243
Identity = 430/486 (88.48%), Postives = 452/486 (93.00%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKW SG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGIII 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDAEDRLAESQPVQEVSDEAAVRLLSESSP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSD EDRL E QP QEV +EA VRLLSESS 
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SSIIAQ 486
           S+I  Q
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. ExPASy TrEMBL
Match: A0A0A0K2Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 2.9e-242
Identity = 429/486 (88.27%), Postives = 449/486 (92.39%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER  DAKI  KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKW SG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWLV++YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE+DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRI+KPDLNRPY+VPLQTFG T+LC PP ALL LVMCLASA+TFLISGIII 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDAEDRLAESQPVQEVSDEAAVRLLSESSP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TL D EDRL ESQ  QEV +EA VRLLSESS 
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSESSS 480

Query: 481 SSIIAQ 486
           S+I  Q
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. ExPASy TrEMBL
Match: A0A6J1I9Z5 (probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC111471008 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.5e-241
Identity = 432/486 (88.89%), Postives = 449/486 (92.39%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER   AKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDER---AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKW SGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVF+H+ ARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSL PF VMTILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLV+DYS VNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSS+WSDGYFAE GALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LELAAF+KLRIKKPDL+RPY+VPLQTFGA MLCLPP ALLVLVMCLASAQTFLISGIII 
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIIS 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDAEDRLAESQPVQEVSDEAAVRLLSESSPS 480
           VGFLLYP+L QAKNR W KFIS Q   TL D ED L E QP QEV DEA +RLLSES  S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDEAVIRLLSESPSS 480

Query: 481 SIIAQQ 487
             IA Q
Sbjct: 481 PNIANQ 483

BLAST of Lcy08g001470 vs. ExPASy TrEMBL
Match: A0A6J1GKM4 (probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111455227 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.6e-240
Identity = 428/486 (88.07%), Postives = 447/486 (91.98%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER   A I  KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF++FPFIWS
Sbjct: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKW SGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVF+H+ ARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSL PF VMTILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLV+DYS VNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSSEWSDGYFAE GALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LELAAF+KLRIKKPDL+RPY+VPLQTFGA MLCLPP ALLVLVMCLASAQTFLISG+II 
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDAEDRLAESQPVQEVSDEAAVRLLSESSPS 480
           VGFLLYP+L QAKNR W KFIS Q   TL D ED L E QP QEV DE+ +RLLSES  S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSS 480

Query: 481 SIIAQQ 487
             IA Q
Sbjct: 481 PNIANQ 483

BLAST of Lcy08g001470 vs. NCBI nr
Match: XP_038888395.1 (probable polyamine transporter At3g19553 [Benincasa hispida])

HSP 1 Score: 868.6 bits (2243), Expect = 2.5e-248
Identity = 438/486 (90.12%), Postives = 456/486 (93.83%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER +DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRADAKIAQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKW SGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIP LLGITASLTYLNYRGLHIVG SAVVLA FSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHVFARIPTLLGITASLTYLNYRGLHIVGVSAVVLAAFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPKKWLV++YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALESDSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFA+RSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMLPSVFAARSKYGTPTFSILCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRIKKPDLNRPYRVPLQTFG TMLC PP ALLVLVMCLASA+TFLISGII+ 
Sbjct: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVT 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDAEDRLAESQPVQEVSDEAAVRLLSESSPS 480
           VGFLLYPTLLQAKN+RW KFIS QP ATL D EDRL ES   QEV +EA VRLLSESS S
Sbjct: 421 VGFLLYPTLLQAKNKRWVKFISEQPEATLPDVEDRLVESHAQQEVPNEAEVRLLSESSSS 480

Query: 481 SIIAQQ 487
           S IAQQ
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. NCBI nr
Match: KAA0031359.1 (putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 852.8 bits (2202), Expect = 1.4e-243
Identity = 431/486 (88.68%), Postives = 452/486 (93.00%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKW SG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGIII 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDAEDRLAESQPVQEVSDEAAVRLLSESSP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSD EDRL E QP QEV +EA VRLLSESS 
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SSIIAQ 486
           S+I  Q
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. NCBI nr
Match: XP_008455023.1 (PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo])

HSP 1 Score: 851.7 bits (2199), Expect = 3.2e-243
Identity = 430/486 (88.48%), Postives = 452/486 (93.00%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER  DAKIA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKW SG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWL+L+YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRI+KPDLNRPY+VPLQT G T+LC PP+ALL+LVMCLASA+TFLISGIII 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDAEDRLAESQPVQEVSDEAAVRLLSESSP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TLSD EDRL E QP QEV +EA VRLLSESS 
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SSIIAQ 486
           S+I  Q
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. NCBI nr
Match: XP_004136943.1 (probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypothetical protein Csa_017296 [Cucumis sativus])

HSP 1 Score: 847.4 bits (2188), Expect = 6.0e-242
Identity = 429/486 (88.27%), Postives = 449/486 (92.39%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER  DAKI  KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKW SG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVFNH+ ARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSL PF VMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
            PKKWLV++YSKVNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALE+DSSEWSDGYFAE GALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LE AAF+KLRI+KPDLNRPY+VPLQTFG T+LC PP ALL LVMCLASA+TFLISGIII 
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPA-ATLSDAEDRLAESQPVQEVSDEAAVRLLSESSP 480
           VGFLLYPTLLQAKNRRW KFIS QP   TL D EDRL ESQ  QEV +EA VRLLSESS 
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSESSS 480

Query: 481 SSIIAQ 486
           S+I  Q
Sbjct: 481 SNIAQQ 486

BLAST of Lcy08g001470 vs. NCBI nr
Match: XP_023553804.1 (probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.5 bits (2183), Expect = 2.3e-241
Identity = 430/486 (88.48%), Postives = 450/486 (92.59%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           MG+ER   A IA KLTILPLIALIFYDVSGGPFGVEDSVS GGGPLLALLGF+VFPFIWS
Sbjct: 1   MGDER---ANIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKW SGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
           RSFPVF+H+ ARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSL PF VMTILSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RP+KWLV+DYS VNWRGYFNSMFWNLNYWDKASTLAGE+ENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSSEWSDGYFAE GALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA GVIF+SW+SFQEILEFLNFLYSIGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIV 420
           LELAAF+KLR+KKPDL+RPY+VPLQTFGA MLCLPP ALLVLVMCLASAQTFLISG+II 
Sbjct: 361 LELAAFIKLRMKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLQAKNRRWAKFISVQPAATLSDAEDRLAESQPVQEVSDEAAVRLLSESSPS 480
           VGFLLYP+L QAKNR W KFIS +  ATL D EDRL E QP +EV DEA +RLLSES  S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISDEQGATLPDVEDRLVEPQPREEVLDEAVIRLLSESPSS 480

Query: 481 SIIAQQ 487
             IA Q
Sbjct: 481 PNIANQ 483

BLAST of Lcy08g001470 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 712.6 bits (1838), Expect = 2.1e-205
Identity = 355/477 (74.42%), Postives = 410/477 (85.95%), Query Frame = 0

Query: 1   MGEERGSDAKIAHKLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWS 60
           + +E  S  K + KLT+LPL+ LIFY+VSGGPFGVEDSV  GGGPLLALLGF++FP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFP+NGGYVVWIS+AFGPFWGFQEGFWKW SGVMDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHLLARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRI 180
            SFPV +H+ AR+PALL IT SLTYLNYRGLHIVGFSAVVLAVFSL PF VM +L++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 RPKKWLVLDYSKVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSS 240
           RPK+WL +D  K+NWRGYFN+MFWNLNYWDKASTLAGE++ P KTFPKA+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALESDSS-EWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL S +S EWSDGYFAE G LIGGVWLK WIQAAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGM 360
           A+QLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIF+SW+SFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLELAAFVKLRIKKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIII 420
           LLE AAFVKLRIKKPDL+RPYRVPL TFG +MLCLPP+ L++LVM LA+ +TFLISG+II
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 VVGFLLYPTLLQAKNRRWAKFI--SVQPAATLSDAEDRLAESQPVQEVSDEAAVRLL 475
           V+GF LYP L   K ++WA+FI    +P + +S      +ESQ  +E  DE+A  LL
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEETRPVSGVS------SESQLDEEHGDESAASLL 478

BLAST of Lcy08g001470 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.1e-153
Identity = 251/427 (58.78%), Postives = 333/427 (77.99%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGFV+FPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW+SGV+DNALYPVLFLDYLK   P     L R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PFAVM ++SIP++ P +WLV+D   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGE+ENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++    +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ +SWLSFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PPT L+  V+ L+S +   +S +++++GFL++P L    
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 434 NRRWAKF 441
            +RW KF
Sbjct: 468 RKRWVKF 473

BLAST of Lcy08g001470 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.1e-153
Identity = 251/427 (58.78%), Postives = 333/427 (77.99%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGFV+FPFIWSIPEAL+TAE+ T 
Sbjct: 32  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 91

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW+SGV+DNALYPVLFLDYLK   P     L R+
Sbjct: 92  YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 151

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PFAVM ++SIP++ P +WLV+D   V
Sbjct: 152 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 211

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++FWNLNYWD  STLAGE+ENP+ T PKA+F  V+LV  SY+ PLLAG GA+ 
Sbjct: 212 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 271

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            +  +W+DGYF++    +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 272 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 331

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
             FA RS+YGTP   IL SA GV+ +SWLSFQEI+   N LY +GM+LE  AFV++R+K 
Sbjct: 332 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 391

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PPT L+  V+ L+S +   +S +++++GFL++P L    
Sbjct: 392 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 451

Query: 434 NRRWAKF 441
            +RW KF
Sbjct: 452 RKRWVKF 457

BLAST of Lcy08g001470 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 251/429 (58.51%), Postives = 329/429 (76.69%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATS 73
           K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF+VFPFIWSIPEAL+TAE+ T 
Sbjct: 52  KITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGTM 111

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           FP+NGGYVVW++ A GP+WGFQ+G+ KW+SGV+DNALYP+LFLDYLK   P+    + R+
Sbjct: 112 FPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRV 171

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLD--YS 193
            A+L +T +LTYLNYRGL IVG +AV+L VFS+ PF VM+ +SIP+++P +WLV+     
Sbjct: 172 AAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMK 231

Query: 194 KVNWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGA 253
            VNW  Y N++FWNLNYWD  STL GE+ENPSKT P+A+F A++LVV SY+ P+L GTGA
Sbjct: 232 GVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGA 291

Query: 254 LESDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGM 313
           +  D   W+DGYFA+ G +IGGVWL WWIQAAAA SNMG+F AEMSSD++QLLGM+E GM
Sbjct: 292 IALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGM 351

Query: 314 IPSVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRI 373
           +P VFA RS+Y TP   IL SA GVI +SWLSFQEI+   N LY  GM+LE   FV+LR+
Sbjct: 352 LPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRM 411

Query: 374 KKPDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQ 433
           K P  +RP+++P+   G+ ++C+PPT L+ ++M   + +  L+S   IV+G +L P L Q
Sbjct: 412 KYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQ 471

Query: 434 AKNRRWAKF 441
            + + W KF
Sbjct: 472 VEKKGWLKF 479

BLAST of Lcy08g001470 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 502.3 bits (1292), Expect = 4.4e-142
Identity = 243/427 (56.91%), Postives = 321/427 (75.18%), Query Frame = 0

Query: 14  KLTILPLIALIFYDVSGGPFGVEDSVSCGGGPLLALLGFVVFPFIWSIPEALVTAELATS 73
           K+++LPL+ LIFY+VSGGPFG E SV+   GPLLALLGFV+FPFIW IPEAL+TAE++T 
Sbjct: 35  KVSMLPLVFLIFYEVSGGPFGAEGSVN-AAGPLLALLGFVIFPFIWCIPEALITAEMSTM 94

Query: 74  FPQNGGYVVWISAAFGPFWGFQEGFWKWISGVMDNALYPVLFLDYLKRSFPVFNHLLARI 133
           FP NGG+VVW+S+A G FWGFQ G+ KW+ GV+DNALYPVLFLDYLK + P     L R+
Sbjct: 95  FPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPALATGLPRV 154

Query: 134 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLFPFAVMTILSIPRIRPKKWLVLDYSKV 193
            ++L +T  LTYLNYRGL IVG++AV + VFS+ PFAVM+++SIP++ P +WLV+D   V
Sbjct: 155 ASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWLVMDLGNV 214

Query: 194 NWRGYFNSMFWNLNYWDKASTLAGEIENPSKTFPKAMFGAVVLVVSSYLIPLLAGTGALE 253
           NW  Y N++ WNLNYWD  STLAGE+ NP KT PKA+   V+ V  S  +PLL+GTGA+ 
Sbjct: 215 NWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLLSGTGAIP 274

Query: 254 SDSSEWSDGYFAEAGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 313
            D   W+DGY AE    IGG WL+ W+QAAAA SNMG+F AEMSSD++QLLGM+E+G++P
Sbjct: 275 LDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGMAELGILP 334

Query: 314 SVFASRSKYGTPTFSILCSAFGVIFVSWLSFQEILEFLNFLYSIGMLLELAAFVKLRIKK 373
            +FA RS+YGTP   IL SA GV+ +S LSFQEI+   N LY  GM+LE  AFV+LR K 
Sbjct: 335 EIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAFVRLRKKH 394

Query: 374 PDLNRPYRVPLQTFGATMLCLPPTALLVLVMCLASAQTFLISGIIIVVGFLLYPTLLQAK 433
           P  +RPY++P+ T G+ ++C+PP  L+ LV+ L++ +  L+S +++V+GFL+ P L    
Sbjct: 395 PAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGFLMKPCLNHMD 454

Query: 434 NRRWAKF 441
            ++W KF
Sbjct: 455 GKKWVKF 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LH393.0e-20474.42Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9C6S51.6e-15258.78Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9FFL12.8e-14958.51Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
A2X8M81.3e-14656.76Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D01.3e-14656.76Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3C4H26.9e-24488.68Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C0141.5e-24388.48probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0K2Y22.9e-24288.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1[more]
A0A6J1I9Z52.5e-24188.89probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1GKM44.6e-24088.07probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038888395.12.5e-24890.12probable polyamine transporter At3g19553 [Benincasa hispida][more]
KAA0031359.11.4e-24388.68putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative p... [more]
XP_008455023.13.2e-24388.48PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo][more]
XP_004136943.16.0e-24288.27probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypotheti... [more]
XP_023553804.12.3e-24188.48probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G19553.12.1e-20574.42Amino acid permease family protein [more]
AT1G31830.11.1e-15358.78Amino acid permease family protein [more]
AT1G31830.21.1e-15358.78Amino acid permease family protein [more]
AT5G05630.12.0e-15058.51Amino acid permease family protein [more]
AT1G31820.14.4e-14256.91Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 13..446
e-value: 6.5E-56
score: 191.8
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 1..454
e-value: 2.5E-66
score: 221.7
NoneNo IPR availablePANTHERPTHR45826:SF21BNAA05G20530D PROTEINcoord: 5..474
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 20..422
e-value: 5.2E-32
score: 111.3
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 5..474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g001470.1Lcy08g001470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity