Lcy08g001360 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g001360
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptiondual-specificity RNA methyltransferase RlmN
LocationChr08: 1856767 .. 1863331 (-)
RNA-Seq ExpressionLcy08g001360
SyntenyLcy08g001360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCGTTTCGATCTGCATTCGATGCCGGAGAGATGAGAACCGAGTTCGAGAAGGCTGGCATCAAATCCACCTTCATCCCCTTCATCTGGAAGCACTTGCTTCACGATCCAAACAACCTATTCAACAATAATCTGACATCCGAATTCGAATGGGAGCAGGCCGTGCCTTCGCTGCCGTCGGTGGCCTACTCCTTGCTCCGATCAAAGTTCAAGCCTCTTACCTCCACTGTCCATTCCGTCGTCGATTCCTCCGATCAGGTCACCACCAAGCTCCTTATCAAGCTTCAGGTGCCCTCCTCTTCCTTCCGCCCATCTCTTTTTCTTTTGGGAAAAGTTCAATTTGTACCCTAGACTTATCAGAATGAGTCAATTTCATCAATTATAATACTGAACGTGATTTAAATCTTTGTTTCTCTAATTATGTTTATCCTTTTAATTGGTTGGGTTTGAGCACTTTGTTGCTAAGTAGGATGTTTTTTTATCTGCTTGGTGGTGAGGCTAAGTTCAAATATTTTCGGATGGGATTTGAGCTTTCTTGTTAACATGACTTTGGTTATATTGTTAGGGTGAAAATTGAACTTTTCCTTTCTTTTGTGTTATCATTCATCTGAACAAAGGTTTGGCAATTAAGAAAATTGATTGGGTAATGATAGTTCAGATCAAGCTGTGAGTTTCTTTTGATTATTGCAGTATTCTTCCGATTATAGTTGTTTTTCAAGTATCTCCAATGTAGTTAATTTTACCTCATGCCTATACGTATGCATTTTTGTTTTTTTCGCTTGTGTTGCTAGAGCGGGGCTTTTGTGGAGGCTGTCATCATGAGGTATGATACTCGTTTGGGAAAATATGGCGGCAAACCTCGTCCAGGTGGTCTGCGGTCTACTCTCTGTATTTCTTCGCAGGTTTTATTTTCTTCATCTCTAATCTGCATTGTCAAAATCCTTTGATTTTATCTTTCCTGATCTTTCGTTGCAAGTCTCTCACTGTCTATCTATTCTCAGGTTGGCTGCAAAATGGGCTGCAGATTCTGTGCAACCGGAACGATGGGATTTAAAAGCAATCTTACTTCAGGGGAGATATTAGAGCAGTTGGTTCATGCCTCTCGTTTTTCCCAAATACGCAATGTCGTTTTCATGGTGATCTTTCCGCCCTCTCATTTGTCTGAATTTCTATTATAGTGACAAATTTCCATTCAGAGGCGGATCACACGAATTAATGCACACTCCGTGATCCTTTGGATGCATCATTATGTGATTTCTATTTAACTATTTTTTAGTTTTTTCTACTCGGCACGTGGATGCCTAGACCGTATCACTTCTTCGCCTACATGGTGAAGCATTCTTGTTGTCCAAAACTTCTAGACTTGTTAGGTTTATTTTGAAAATTGGATAATTTTCTCGGGAGTTACAATACTGTGGACAAATGTAGTGACTGTTGACAGAGTGTCCATGATAGGAACTAAGATATTGTTGCTTTTTTTTTTTAATAATGAACCACACTTTCATTGAGAAAAAATGAAAGAATACAAGGGCGAACAAAAAACCAAGTCTCCAAAAAGAGACCTCTTAGAAAAAGGGACTCCAATCCAGAAGAATGATACCTATAGGATTATTTCAAAGTAAGCGCGTCACCAACGCCTAGAGAGAGACATAATATCTAGCAAGGGACCAAACATCATCAGTATTCCTTTCCATCCCTCTAAAGGTCCTATTGTTTCTCTCACCCCATAACCCCCAAACAATAGCACAAATCCCAGTCTACCGCAAGAAATTTTCCTGTTCCTGAAAAGGAGGATGGGGAAGAAACTCCTCAATTATCTCCCTGAGGCCCTAATCTGTGAAGGGAACTTCAAGCATAGCTCTTTGCTCAATTCTAAAGGCAGTGAATGATTTAGGCACTAACAAGACCCCGGGGCCCGACGGTTATACAGCGGATTTCTTCAAAAAATCGTGGAACATCCTCAAGGATGATATCAAGGGTGTGTTCAATGATTTTTTCCAGAATGGGATCATTAATGCAAGCTTAAGTGAGACTTGCATCTGCCTTATTCCTAAGATTATTGGGGCCAAAAAAGTGGGAGACTTTAGGCCTATAAGCCTAACAACTTGCCTTTACAAGGTTATTGCTCGGGTCCTATCAGATAGGTTAAAAAGAGTTTTACCAGATACAATCACAGAATTTCAATCAGCCTTTGTTGGTAATTGACAAATCATGGATGCCTCCCTAATTGCCAACGAGTTGATTGATGAATGGAAGAGGAAGAAACGAAAGGGTGTGGTGATCAAGCTGGACATTGCAAAAGCTTTTGACACGGTTGATTGGGATTTCCTCGACGATATTTTGAAAGCTAAAGGCTTTGGTCATCTGTGGAGGAAATGGATCAGAGGTTGTATATCATATGCCAATTTCTCGATTATTATCAATGGTAAACCGAGGGGAAAAATCTTAGCATTGAGGGGCCTTAGACAAGGGGACCCACTATCCCCCTTTCTATTCATTTTAGTAGCTGACTGTCTTAGTCGAATGCTTCTTAAGGCTGAACAGGATGCCTCCATTGAAGGTTTTTGGGTTGGTGAAGGTCCACAAGCCATTAAAACCACTCATCTCCAGTTTGCAGACGACACCATCCTTTTTTCATCCCATAAGACGTCATGCATCTCTAACCTTTTCAATGTTATTCGAAAATTTGAAGCCGCATCAGGATTGAGTATTAATTGCAACAAGACTGAATTTTTGGGCCTAGGTATTGATTCACAGCAAGCTGGCACACTTAGTATCTATGTTTGGATGCAAACAGGGTGCTTGGCTAATTACCTATTTGGGTCTTCCCCTAAACGGTAATCCAAGAAGATCTAATTTTTGGACTCCAGTGATTGAAAAGGTCCAGAAACGACTGCATAATTGGGGATCCACCAACATCTCCAAAGGAGGTAGACACACCCTTATTCAAGCTACCCTCACAAACCTTCCCATATATTACCTCTCCCTATTCCAAGCCCCAAAGAAGGTCACCAAAGCCATAGAGAAACTTTACCGTGCGTTCCTTTGGAGAGGAGGTAGTGAGAAAAAAGGTTGTCATCTCCTGAAATGGTCCCATCTACAACTCCCAAAGGAAGAAGGCGGCCTAGGCATCTATGATATTCATAAGAAAAATGTCTCCCTATTAGCCAAATGGGTTTGGAGATTTTATCAAGAACCGAATGCTCTATGGAGGAAAATCATTGTCACTAAATATGGCTCAGCTAACAACCCCTATAAATTGGGTGATCAATCCTTGAGTTCATCCAAAGGCTCATGGAGAGCTATCCACAAGTCTAAATACCTTATATATGACTACATTGACATTAGAGTTGGGAAAGGTGACAAAACGCTCTTTTGGGAGGATATTTGGCTGAGAACTTCTCCTCTAAAGTCCACTTATCCCTCTCTTTATAGTCTATCGCTCAAAAAGGATGCTCTCATTGCTGATTTATGGAATCCAACCGATGGGGCATGGAACCTACACCTAAGAAGATACCTCCAGGAATCTGAAATTATGGAATGGGCATTGTTAACTCACCAATTGTCATCCTTTTCCTTCAGAAATACTGCAGACTCATGGGTTTGGAAGCTTGAACAAAATGGCTCTTTTTCTAGCAGGGTGATTTTTTAACAGCAGGTCCCTCACCCGTAAACTAGCATCATCTAATCACCATTTCAGCAGTGATCTATACGGCCAGTTGTGGAAGGGTCTTTTGCCGAAGAAAGTCAAGTTTTTCATATGGGAACTTAGCCATCAATGTATTAATACAGCGGACATTGTTCAGAGAAGATACCCCGATTCATCTTTATCTCCTAGCTGGTGGAGCCTGTGTAACAAGGCCGCCGAGACCCAAATTCACCTTTTCAGTCGCTGTGAATACGCTGCTGCTTTTTGGGACCACATTCAAGATGCTTTTGGCAATTTGCTCGTCCGGGTGATATCCCATCCCTTCTTCATTTCACTCTTCTTGGTCACCCTTTCAAAAACGATACTAAGGTTCTTTGGCGGAATTTTTTGTATGCCTTCTTCTGGAACTTATGGCTAGAAAGAAATGCTAGAATCTTCGATAATCAGCAGCATCATGTTTATGCTTTTATAGAATCTACATCTCATCTTGCCATGTATTGGAGTAGTCAAATCTCCCCTTTTTGTAATTATCCCTTATCTTCCCTTATATCCCAATGGAGATCTCTTTTGTAATTATCAGATAGGACCTCTTTTGTATCTATTTCATACATCAATGAAATAGTTTCGTTTCCTATCAAAAAAAAAAAAAAAAAAAAACTCTCCCTGAGGCCCTTGTGCCCAGCAAACTACAAGCCGAAAACCCAAACAAAGTGTTCCAAACCGAGGAACTAAGATATTGTTGCTGGCTTAGTTCGAGCATTCGACCTTACAAGTCCATGGAGCTTTCCTTTTCATTATTTTCTTAGCAGCTTGTGGCCCTATATCTGTTAAATTGTTCTCTGTTTCCTTGTTAGTAAGAAGTTGCTTGCTTCATGCTTTCTTATCGTTTCATATGATTTTTTTTTTCTGTTCTCAGAATATACTTTTTTCATGCTGATTTTATTATTTTGTGACACAGGGAATGGGAGAACCATTGAATAACTATTCTGCCTTAGTGGAAGCTGTCCGTGCCATGACAGGCTCACCATTCTTGTTATCACCCAAGAGAATTACTATATCAACTGTATGTACTCTGATCTACCGGTTTTCTACTTCTATGGTCTTTATAGCTAAGTTTGTATAATTTATAAGGTTTCACCTTTTTCCCTTGGCTCTCATCATAGTGTTGATTTTTTTTTTCTTTTTCCTTTTTCATTCTTTCAAGAGACATTGTATTCACAGTAGAATATTGAAAGTGAGAGGAAGCTAGACATCCCATCCATGCAGTGGTATATGAGGATAAGAAGCCATCCAATTTGTAATAATTTTAACAATGGAAGTGGGAAACTGCAATTACCCAACTAGAAACATCAAATTGTACATGATTCTATTAGTTTAGTGTGATTATATCTACACATCACCTGGCTTTGTCCTTACATGGGATTTAGGTTCACCTACATCTCACAGGACTTGGCAGAAAACCCACAAAGTTTCCTTAAGTTTCTCATAGAAACTACATTGGGGATGAAAAGGTGGTCAATACTTTCTCTCTCTCTTCCACAAGGTCTGGTGCTCACATATCTAAGGATTGAGTACAAATTGAGATATCTTGAGGTATTCACTCATTAGCAGTGACCTTACAAAAAACCTGTGGTGTGCCACACACTTAGTATTTGCTTATTCATTTGGAGTAATTCCTGTTTGAATTTTGAGAACTACTAAAACGCTTCTCCTTCATTCCACTTGACAACATCCAAATATACTTCATCATTTAAGCTCAACATACTTTAGAAAAGATTTTAAAAAAATGAATTTATGCATTTATTATTAAGTTTCATGGGCAGTGAGCTTGCTGTCCGAGAATAGTGCTTGTTATGAAGATGGGCCTTTGCATCTGAGAATTTTTTTTTATAATAATTTTTCTTGATGAAGTCCGATCCTAAATATTTGTTATAAAATAATGTAATGGGAGTCCAGAGAGTCGGTTTCTATCCTCTTATCCGTTTCCTCTTCCTTCGGGTGCTGTTTAACTGTTAAAAGAAGGTGACTAGTCGAAGTTGTACTCTGTTCGTATTTCTGCAGGTTGGTATTATTCATGCTATAAACAAACTGCATGGTGATCTACCAGGATTGAACTTGGCCGTTTCCTTACATGCACCTGTTCAAGATATTCGATGTCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTACAAAAACTTATGGATGCACTCCAAGAATATCAAAGAAAGAGGTAAATATTCCATTTTTGCTTTTGCCAAAGATTTTCAATACATTGCCCCTTCTCTAATGTAAAAAAGCATGTTGTGTCTATGGTTTCAGTCAGCAAAAGATATTAATTGAGTACATAATGCTTGATGGGGTGAACGATGAAGAGCAACATGCCCACCAACTTGGAAAATTGTTAGAGACATTCCAAGTGGTAAGCTCTGAAATTGGTCAGCTTTACTCTTTTCCTTGTTTAAATCTCAATGTTAATTGGAGCTCTGGATGTCGAGTCATTTAAATACGCTGGTATCAAACTACATTTTCTTTTATCATGATTATTTGCATGGTGCAAGTAAGTAAATGTGTGTAAGAAAGTTTCTTGGAGATTTCATTTGTTGTCACCACCTCATGATTCAAGTACCTTTGTTTGCTTATGATGCGACTAAATTTTACCTTTCTGGCAGGTTGTGAACTTAATACCATTCAATCCTATTGGCAGTTCAAGTCAATTCAGAACCAGTAGTGATGAGAAAGTTTCAATATTCCAGAAAGTACTTCGCGGTACGTATAATATACGCACAACAGTTCGTAAGCAAATGGGTCAAGATATTAGTGGTGCATGTGGGCAACTGGTTGTCACCCTGCCTAATAGAAGCTCCGGTAAACAAACAAGTGTTCTACCTGACATTGAAGATCTTCACCTCTGA

mRNA sequence

ATGGCGGCGTTTCGATCTGCATTCGATGCCGGAGAGATGAGAACCGAGTTCGAGAAGGCTGGCATCAAATCCACCTTCATCCCCTTCATCTGGAAGCACTTGCTTCACGATCCAAACAACCTATTCAACAATAATCTGACATCCGAATTCGAATGGGAGCAGGCCGTGCCTTCGCTGCCGTCGGTGGCCTACTCCTTGCTCCGATCAAAGTTCAAGCCTCTTACCTCCACTGTCCATTCCGTCGTCGATTCCTCCGATCAGGTCACCACCAAGCTCCTTATCAAGCTTCAGAGCGGGGCTTTTGTGGAGGCTGTCATCATGAGGTATGATACTCGTTTGGGAAAATATGGCGGCAAACCTCGTCCAGGTGGTCTGCGGTCTACTCTCTGTATTTCTTCGCAGGTTGGCTGCAAAATGGGCTGCAGATTCTGTGCAACCGGAACGATGGGATTTAAAAGCAATCTTACTTCAGGGGAGATATTAGAGCAGTTGGTTCATGCCTCTCGTTTTTCCCAAATACGCAATGTCGTTTTCATGGGAATGGGAGAACCATTGAATAACTATTCTGCCTTAGTGGAAGCTGTCCGTGCCATGACAGGCTCACCATTCTTGTTATCACCCAAGAGAATTACTATATCAACTGTTGGTATTATTCATGCTATAAACAAACTGCATGGTGATCTACCAGGATTGAACTTGGCCGTTTCCTTACATGCACCTGTTCAAGATATTCGATGTCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTACAAAAACTTATGGATGCACTCCAAGAATATCAAAGAAAGAGTCAGCAAAAGATATTAATTGAGTACATAATGCTTGATGGGGTGAACGATGAAGAGCAACATGCCCACCAACTTGGAAAATTGTTAGAGACATTCCAAGTGGTTGTGAACTTAATACCATTCAATCCTATTGGCAGTTCAAGTCAATTCAGAACCAGTAGTGATGAGAAAGTTTCAATATTCCAGAAAGTACTTCGCGGTACGTATAATATACGCACAACAGTTCGTAAGCAAATGGGTCAAGATATTAGTGGTGCATGTGGGCAACTGGTTGTCACCCTGCCTAATAGAAGCTCCGGTAAACAAACAAGTGTTCTACCTGACATTGAAGATCTTCACCTCTGA

Coding sequence (CDS)

ATGGCGGCGTTTCGATCTGCATTCGATGCCGGAGAGATGAGAACCGAGTTCGAGAAGGCTGGCATCAAATCCACCTTCATCCCCTTCATCTGGAAGCACTTGCTTCACGATCCAAACAACCTATTCAACAATAATCTGACATCCGAATTCGAATGGGAGCAGGCCGTGCCTTCGCTGCCGTCGGTGGCCTACTCCTTGCTCCGATCAAAGTTCAAGCCTCTTACCTCCACTGTCCATTCCGTCGTCGATTCCTCCGATCAGGTCACCACCAAGCTCCTTATCAAGCTTCAGAGCGGGGCTTTTGTGGAGGCTGTCATCATGAGGTATGATACTCGTTTGGGAAAATATGGCGGCAAACCTCGTCCAGGTGGTCTGCGGTCTACTCTCTGTATTTCTTCGCAGGTTGGCTGCAAAATGGGCTGCAGATTCTGTGCAACCGGAACGATGGGATTTAAAAGCAATCTTACTTCAGGGGAGATATTAGAGCAGTTGGTTCATGCCTCTCGTTTTTCCCAAATACGCAATGTCGTTTTCATGGGAATGGGAGAACCATTGAATAACTATTCTGCCTTAGTGGAAGCTGTCCGTGCCATGACAGGCTCACCATTCTTGTTATCACCCAAGAGAATTACTATATCAACTGTTGGTATTATTCATGCTATAAACAAACTGCATGGTGATCTACCAGGATTGAACTTGGCCGTTTCCTTACATGCACCTGTTCAAGATATTCGATGTCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTACAAAAACTTATGGATGCACTCCAAGAATATCAAAGAAAGAGTCAGCAAAAGATATTAATTGAGTACATAATGCTTGATGGGGTGAACGATGAAGAGCAACATGCCCACCAACTTGGAAAATTGTTAGAGACATTCCAAGTGGTTGTGAACTTAATACCATTCAATCCTATTGGCAGTTCAAGTCAATTCAGAACCAGTAGTGATGAGAAAGTTTCAATATTCCAGAAAGTACTTCGCGGTACGTATAATATACGCACAACAGTTCGTAAGCAAATGGGTCAAGATATTAGTGGTGCATGTGGGCAACTGGTTGTCACCCTGCCTAATAGAAGCTCCGGTAAACAAACAAGTGTTCTACCTGACATTGAAGATCTTCACCTCTGA

Protein sequence

MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLPSVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMGMGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQLVVTLPNRSSGKQTSVLPDIEDLHL
Homology
BLAST of Lcy08g001360 vs. ExPASy Swiss-Prot
Match: A0LQM1 (Dual-specificity RNA methyltransferase RlmN OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=rlmN PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.6e-54
Identity = 121/284 (42.61%), Postives = 177/284 (62.32%), Query Frame = 0

Query: 83  DSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGGLRSTLCISSQVGCKMGCR 142
           + +D  T+K L  L+ G  +EAV++              P   RSTLC+SSQVGC +GC+
Sbjct: 72  EQADDGTSKYLFGLRDGHSIEAVLI--------------PDLPRSTLCVSSQVGCALGCK 131

Query: 143 FCATGTMGFKSNLTSGEILEQLVHASR----FSQIRNVVFMGMGEPLNNYSALVEAVRAM 202
           FC TG++GFK NL++ EI++Q+    R     S+I N+VFMGMGEPL N  +++ A+R +
Sbjct: 132 FCLTGSLGFKRNLSAAEIVDQVCQVQRDLGSRSRITNIVFMGMGEPLANLDSVLRAIRVI 191

Query: 203 T-GSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQDIRCQIMPAARAFPL 262
              +    S +RIT+ST G++  + +L  + P +NLAVSLHA   ++R ++MP  R +PL
Sbjct: 192 AEPNGMAFSHRRITLSTAGLVPQLRRLGRESP-VNLAVSLHAAENELRAELMPVNRTYPL 251

Query: 263 QKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQVVVNLIPFNPIGSS 322
           + LM A +EY    +++I  EYI+LDG+ND+ + A QL KLL   +  VNL+PFNP    
Sbjct: 252 EVLMAACREYPLPPRKRITFEYILLDGINDDPKQAKQLVKLLHGIRAKVNLMPFNP-HPG 311

Query: 323 SQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQLV 362
           S FR  S+++V  FQ+ L+    I T VR+  G +I  ACGQLV
Sbjct: 312 SVFRKPSEQRVLAFQEALQNA-RITTHVRRSRGGEIGAACGQLV 338

BLAST of Lcy08g001360 vs. ExPASy Swiss-Prot
Match: Q1DCU1 (Dual-specificity RNA methyltransferase RlmN 1 OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=rlmN1 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.5e-54
Identity = 131/335 (39.10%), Postives = 185/335 (55.22%), Query Frame = 0

Query: 41  LFNNNLTSEFEWEQAVPSLPSVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGA 100
           L+  + T+  E +   P L      +LR   +      H    SSD  T KLL++L  G 
Sbjct: 38  LYRRHATTFDELDGLKPEL----LRMLREHTRLGQLATHHESFSSDGFTHKLLLRLDDGQ 97

Query: 101 FVEAVIMRYDTRLGKYGGKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI 160
            +E V+MR+                R+T+CIS+Q GC MGC FCATG MG   +LT GEI
Sbjct: 98  TIETVLMRFKG--------------RATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEI 157

Query: 161 LEQLVHASRF-----SQIRNVVFMGMGEPLNNYSALVEAVRAMTGSPFL-LSPKRITIST 220
           + Q++H +R        +RNVV MGMGEPL+NY   + AV  +  +  L + P+ IT+ST
Sbjct: 158 VGQILHVNRILRASGETLRNVVLMGMGEPLHNYEHTMSAVDVLVDALGLAMGPRFITLST 217

Query: 221 VGIIHAINKLHGDLPGLNLAVSLHAPVQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQK 280
           VG++  I +L  +   ++LAVSLH      R  ++PA R +PL +LMDA + Y  K +++
Sbjct: 218 VGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRWPLDELMDACRYYSEKRKRR 277

Query: 281 ILIEYIMLDGVNDEEQHAHQLGKLLETFQVVVNLIPFNP-IGSSSQFRTSSDEKVSIFQK 340
           I  E+ ++ G ND  +HAH LG+LL      VN+IP NP +G       S  E V  FQ 
Sbjct: 278 IFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTVGYDG--GPSRPESVRAFQD 337

Query: 341 VLRGTYNIRTTVRKQMGQDISGACGQLVVTLPNRS 369
           VL  TY++ +TVR++ G DI   CGQL  T+  RS
Sbjct: 338 VL-ATYDVPSTVRQRRGIDIDAGCGQLKATVERRS 351

BLAST of Lcy08g001360 vs. ExPASy Swiss-Prot
Match: A9A3L9 (Ribosomal RNA large subunit methyltransferase N OS=Nitrosopumilus maritimus (strain SCM1) OX=436308 GN=rlmN PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.5e-54
Identity = 124/316 (39.24%), Postives = 191/316 (60.44%), Query Frame = 0

Query: 56  VPSLPSVAYSLLRSKFKPLTSTVHSV-----VDSSDQVTTKLLIKLQSGAFVEAVIMRYD 115
           +P LP      LR +F     T+ S      V S D  TTKLL++L  G+ VE V+M+Y+
Sbjct: 44  IPQLP----KKLREEFTEAGYTIGSAKEIHRVVSDDGDTTKLLLELSDGSSVETVLMQYE 103

Query: 116 TRLGKYGGKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVH---- 175
               K GG P     RST+C+S+Q+GC MGC FCATG MGF++NL +  I+ Q++H    
Sbjct: 104 PT--KIGGHP-----RSTICVSTQIGCAMGCVFCATGQMGFETNLKAEHIVSQVIHFAEL 163

Query: 176 -ASRFSQIRNVVFMGMGEPLNNYSALVEAVRAMT-GSPFLLSPKRITISTVGIIHAINKL 235
              R   + N+VFMGMGEP+ NY  ++ AV+ +T    F L  + ITIST+GI   I KL
Sbjct: 164 LEQRGEHVTNLVFMGMGEPMANYDEMIRAVKILTHDRGFGLGQRHITISTIGITSGIEKL 223

Query: 236 HGDLPGLNLAVSLHAPVQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDG 295
             +   + LA+SLHAP  ++R +++P A    ++ ++ + ++Y +K+ +++  EY +++G
Sbjct: 224 AEENLQIGLAISLHAPNNELRKKLVPTAGPNSVEDIIKSGRDYFKKTGRRVTFEYALMEG 283

Query: 296 VNDEEQHAHQLGKLLETFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTT 355
           VND  + AH+L +LL      VN+IP NP  ++  F+  S++ V  F+++LR +  +  T
Sbjct: 284 VNDSPEIAHELARLLRGNGSHVNIIPINP--TAGDFKRPSEKNVLEFEQILRKS-GVNCT 343

Query: 356 VRKQMGQDISGACGQL 361
           VR + G +IS ACGQL
Sbjct: 344 VRVEKGTEISAACGQL 345

BLAST of Lcy08g001360 vs. ExPASy Swiss-Prot
Match: Q6MDD0 (Probable dual-specificity RNA methyltransferase RlmN 2 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=rlmN2 PE=3 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 2.9e-53
Identity = 119/288 (41.32%), Postives = 177/288 (61.46%), Query Frame = 0

Query: 83  DSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGGLRSTLCISSQVGCKMGCR 142
           +S DQ T K L +L+ G  VE+V++                G+R T+C+SSQVGC   C 
Sbjct: 70  ESIDQETIKFLWRLRDGNLVESVLIL--------------SGIRRTVCVSSQVGCPAKCA 129

Query: 143 FCATGTMGFKSNLTSGEILEQLVH-----ASRFSQIRNVVFMGMGEPLNNYSALVEAVRA 202
           FCA+G  GF  NL   EI+EQ++      +S+  ++ +VV+MGMGEPL NY ++V ++R 
Sbjct: 130 FCASGQQGFFRNLRPTEIIEQILQINAWLSSKGEKVSHVVYMGMGEPLKNYESVVASIRV 189

Query: 203 MTGSPFL-LSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQDIRCQIMPAARAFP 262
           ++   F  +S +RIT+STVG++  I +L  +   +NL +SLHAP Q IR +I+P AR +P
Sbjct: 190 LSHPDFCNISQRRITVSTVGVVEGIKRLSKEGLKVNLVLSLHAPNQHIRKKIIPYARKYP 249

Query: 263 LQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQVVVNLIPFNPIGS 322
           L+++++++ EY +K+++ I  EY +L G+ND   HAH+L  LL+  Q  VNLIP+NPI  
Sbjct: 250 LEEILESMDEYAQKTKRDITFEYTLLAGINDHPDHAHELAHLLKGKQCTVNLIPYNPIPG 309

Query: 323 SSQFRTSSDEKVSI--FQKVLRGTYNIRTTVRKQMGQDISGACGQLVV 363
               R    EK +I  F+ VL G++ I  T R   G DI  ACGQL +
Sbjct: 310 ---LRLKRPEKKAIKQFRSVLYGSH-IVNTCRYTKGDDIGAACGQLAL 339

BLAST of Lcy08g001360 vs. ExPASy Swiss-Prot
Match: Q1MQJ3 (Dual-specificity RNA methyltransferase RlmN OS=Lawsonia intracellularis (strain PHE/MN1-00) OX=363253 GN=rlmN PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.5e-52
Identity = 130/326 (39.88%), Postives = 187/326 (57.36%), Query Frame = 0

Query: 54  QAVPSLPSVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRL 113
           +++ +LP    + L +  K     + ++  SSD  T K L++L  GA +E V++      
Sbjct: 43  ESMTNLPQKIRASLTALIKINLPEIVTIQQSSDG-TKKFLLRLSDGALIETVLI------ 102

Query: 114 GKYGGKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQ- 173
                  + G +R T C+SSQVGC MGC FC+T TMGF  NLT+GEI+ Q++ A      
Sbjct: 103 ---PSIDKAGNIRITQCLSSQVGCSMGCTFCSTATMGFIRNLTAGEIVSQVLLAKLHLND 162

Query: 174 -------IRNVVFMGMGEPLNNYSALVEAVRAMTGSPFL-LSPKRITISTVGIIHAINKL 233
                  IRN+VFMGMGEPL N + L  A+  +     L  S +RIT+ST GI   I  L
Sbjct: 163 NKPDKPIIRNIVFMGMGEPLLNLTELTRALHILHSEKGLNFSARRITVSTCGIKKGIQAL 222

Query: 234 HGDLPGLN-LAVSLHAPVQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLD 293
             +  GL  LA+SLHA  Q++R  IMP A  + L++L+D L+ Y  K ++KI  EY++L 
Sbjct: 223 SEN--GLAFLALSLHASNQELRSTIMPKAAKWDLKELIDTLKNYSLKKREKITFEYLLLG 282

Query: 294 GVNDEEQHAHQLGKLLETFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRT 353
           G+ND  +HA +L KL+   +  +NLIP+NP      +   ++E +  FQKVL  +  I T
Sbjct: 283 GINDSPEHAKELAKLITDIKGKLNLIPYNP-AQGQPYLKPTEENILKFQKVL-WSKGIVT 342

Query: 354 TVRKQMGQDISGACGQLVVTLPNRSS 370
            +RK  GQDI+ ACGQL  T  ++SS
Sbjct: 343 ILRKSKGQDINAACGQLKTTYLSQSS 354

BLAST of Lcy08g001360 vs. ExPASy TrEMBL
Match: A0A5D3C8Y4 (Dual-specificity RNA methyltransferase RlmN OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001230 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 2.9e-205
Identity = 358/384 (93.23%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWKHLLHD NNLFN+NL SEF+WEQAVPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDQNNLFNHNLASEFQWEQAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AY LLR KFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SAAYCLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI+EQL+HASRFSQIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLIHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYS+LVEAVRAM G PFLLSPKRITISTVGIIHAINKLH DLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMGPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKILIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQV+VNLIPFNPIGSSSQF+TS DEKVS+FQK+LRGTYNIRTTVRKQMGQDISGACGQL
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSGDEKVSVFQKILRGTYNIRTTVRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+LPNRSS KQ+SVLPDIEDLHL
Sbjct: 361 VVSLPNRSSSKQSSVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. ExPASy TrEMBL
Match: A0A1S3C167 (dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103495308 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 2.9e-205
Identity = 358/384 (93.23%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWKHLLHD NNLFN+NL SEF+WEQAVPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDQNNLFNHNLASEFQWEQAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AY LLR KFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SAAYCLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI+EQL+HASRFSQIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLIHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYS+LVEAVRAM G PFLLSPKRITISTVGIIHAINKLH DLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMGPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKILIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQV+VNLIPFNPIGSSSQF+TS DEKVS+FQK+LRGTYNIRTTVRKQMGQDISGACGQL
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSGDEKVSVFQKILRGTYNIRTTVRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+LPNRSS KQ+SVLPDIEDLHL
Sbjct: 361 VVSLPNRSSSKQSSVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. ExPASy TrEMBL
Match: A0A6J1D562 (uncharacterized protein LOC111017398 OS=Momordica charantia OX=3673 GN=LOC111017398 PE=4 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 9.1e-199
Identity = 348/384 (90.62%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MAAFRS F+AGE+R EFE AGIKSTF+PFIWK LLHDPNN  N++  +EF+WE+AVPSLP
Sbjct: 1   MAAFRSVFNAGELRREFENAGIKSTFVPFIWKRLLHDPNNFLNHDFATEFDWERAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AY+LLRSKFKPLTSTVHSVVDSSDQVT+KLL+KLQ+GAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SPAYALLRSKFKPLTSTVHSVVDSSDQVTSKLLVKLQNGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI+EQLVHASR SQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLVHASRLSQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLH DLPGL LAVSLHAP
Sbjct: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHDDLPGLKLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ++SQQKILIEYIMLDGVNDEE HAH LGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKRSQQKILIEYIMLDGVNDEEHHAHLLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQVVVNLIPFNPIGSSSQFRTSS EKV IFQKVLRGTYNIRTT+RK+MGQDISGACGQL
Sbjct: 301 TFQVVVNLIPFNPIGSSSQFRTSSSEKVLIFQKVLRGTYNIRTTIRKEMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+LP+RSSGKQTS+LPDIEDLHL
Sbjct: 361 VVSLPSRSSGKQTSILPDIEDLHL 384

BLAST of Lcy08g001360 vs. ExPASy TrEMBL
Match: A0A6J1IBJ2 (uncharacterized protein LOC111470995 OS=Cucurbita maxima OX=3661 GN=LOC111470995 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.9e-197
Identity = 349/384 (90.89%), Postives = 364/384 (94.79%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWK LLHD NNL N N+ SEF+WE+AVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S  YSLLRSKFKPLTSTV++VVDSSDQ TTKLLIKLQSGAFVEAVIMRYD+RLGKYG KP
Sbjct: 61  SATYSLLRSKFKPLTSTVNTVVDSSDQTTTKLLIKLQSGAFVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEI+EQLVHASR  QIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYSALVEAVR M GSPFLLSPKRITISTVGIIHAI+KLHGDLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKI IEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TF VVVNLIPFNPIGSSSQF+TSSDEKVS FQ+VLRGTYNIRTT+RKQMGQDISGACGQL
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+  NR SGKQT+VLPDIEDLHL
Sbjct: 361 VVSQTNRISGKQTTVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. ExPASy TrEMBL
Match: A0A6J1GM04 (uncharacterized protein LOC111455593 OS=Cucurbita moschata OX=3662 GN=LOC111455593 PE=4 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.1e-195
Identity = 346/384 (90.10%), Postives = 363/384 (94.53%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIP IWK L+HD NNL N N+ SEF+WE+AVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPSIWKQLIHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AYSLLRSKFKPLTSTV++VVDSSDQ TTKLLIKLQSGA VEAVIMRYD+RLGKYG KP
Sbjct: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEI+EQLVHASR  QIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYSALVEAVR M GSPFLLSPKRITISTVGIIHAI+KLHGDLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KS+QKI IEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSRQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TF VVVNLIPFNPIGSSSQF+TSSDEKVS FQ+VLRGTYNIRTT+RKQMGQDISGACGQL
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+  NR SGKQT+VLPDIEDLHL
Sbjct: 361 VVSQTNRISGKQTTVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. NCBI nr
Match: XP_038888929.1 (dual-specificity RNA methyltransferase RlmN [Benincasa hispida])

HSP 1 Score: 734.6 bits (1895), Expect = 4.5e-208
Identity = 365/384 (95.05%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAG+K+TFIPFIWKHLLHDPNNLFN+NL SEFEWEQAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGVKTTFIPFIWKHLLHDPNNLFNHNLASEFEWEQAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AY LLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SAAYCLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGG RSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEI+EQLVHAS  SQIRN+VFMG
Sbjct: 121 RPGGPRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASHLSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYSALVEAVRAM GSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSALVEAVRAMIGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKILIEYIMLDGVND+EQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDQEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQVVVNLIPFNPIGSSSQFRTS DEKVS+FQKVLRGTYNIRTTVRKQMGQDISGACGQL
Sbjct: 301 TFQVVVNLIPFNPIGSSSQFRTSDDEKVSVFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV++PNRSSGKQTSVLPDIEDLHL
Sbjct: 361 VVSMPNRSSGKQTSVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. NCBI nr
Match: XP_008455036.1 (PREDICTED: dual-specificity RNA methyltransferase RlmN [Cucumis melo] >KAA0031367.1 dual-specificity RNA methyltransferase RlmN [Cucumis melo var. makuwa] >TYK06819.1 dual-specificity RNA methyltransferase RlmN [Cucumis melo var. makuwa])

HSP 1 Score: 724.2 bits (1868), Expect = 6.1e-205
Identity = 358/384 (93.23%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWKHLLHD NNLFN+NL SEF+WEQAVPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDQNNLFNHNLASEFQWEQAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AY LLR KFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SAAYCLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI+EQL+HASRFSQIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLIHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYS+LVEAVRAM G PFLLSPKRITISTVGIIHAINKLH DLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMGPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKILIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQV+VNLIPFNPIGSSSQF+TS DEKVS+FQK+LRGTYNIRTTVRKQMGQDISGACGQL
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSGDEKVSVFQKILRGTYNIRTTVRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+LPNRSS KQ+SVLPDIEDLHL
Sbjct: 361 VVSLPNRSSSKQSSVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. NCBI nr
Match: XP_011658875.1 (uncharacterized protein LOC101215180 [Cucumis sativus] >XP_031744517.1 uncharacterized protein LOC101215180 [Cucumis sativus] >XP_031744518.1 uncharacterized protein LOC101215180 [Cucumis sativus] >KAE8645842.1 hypothetical protein Csa_017286 [Cucumis sativus])

HSP 1 Score: 723.8 bits (1867), Expect = 7.9e-205
Identity = 357/384 (92.97%), Postives = 374/384 (97.40%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWKHLLHDPNN+FN+NL SEF+WEQ VPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDPNNIFNHNLASEFQWEQVVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AYSLLR KFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SAAYSLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI+EQLVHASRFSQIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLVHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYS+LVEAVRAM   PFLLSPKRITISTVGIIHAINKLH DLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMAPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKILIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQV+VNLIPFNPIGSSSQF+TSSDEKVS+FQ+VLRGTYNIRTT+RKQMGQDISGACGQL
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSSDEKVSVFQQVLRGTYNIRTTIRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+LPNRSS KQ+SV+PDIEDLHL
Sbjct: 361 VVSLPNRSSSKQSSVVPDIEDLHL 384

BLAST of Lcy08g001360 vs. NCBI nr
Match: XP_022148839.1 (uncharacterized protein LOC111017398 [Momordica charantia])

HSP 1 Score: 702.6 bits (1812), Expect = 1.9e-198
Identity = 348/384 (90.62%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MAAFRS F+AGE+R EFE AGIKSTF+PFIWK LLHDPNN  N++  +EF+WE+AVPSLP
Sbjct: 1   MAAFRSVFNAGELRREFENAGIKSTFVPFIWKRLLHDPNNFLNHDFATEFDWERAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AY+LLRSKFKPLTSTVHSVVDSSDQVT+KLL+KLQ+GAFVEAVIMRYDTRLGKYGGKP
Sbjct: 61  SPAYALLRSKFKPLTSTVHSVVDSSDQVTSKLLVKLQNGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEI+EQLVHASR SQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLVHASRLSQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLH DLPGL LAVSLHAP
Sbjct: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHDDLPGLKLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
           VQDIRCQIMPAARAFPLQKLMDALQEYQ++SQQKILIEYIMLDGVNDEE HAH LGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKRSQQKILIEYIMLDGVNDEEHHAHLLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQVVVNLIPFNPIGSSSQFRTSS EKV IFQKVLRGTYNIRTT+RK+MGQDISGACGQL
Sbjct: 301 TFQVVVNLIPFNPIGSSSQFRTSSSEKVLIFQKVLRGTYNIRTTIRKEMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+LP+RSSGKQTS+LPDIEDLHL
Sbjct: 361 VVSLPSRSSGKQTSILPDIEDLHL 384

BLAST of Lcy08g001360 vs. NCBI nr
Match: XP_023553823.1 (uncharacterized protein LOC111811282 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.8 bits (1810), Expect = 3.2e-198
Identity = 351/384 (91.41%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWK LLHD NNL N N+ SEF+WE+AVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SVAYSLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120
           S AYSLLRSKFKPLTSTV++VVDSSDQ TTKLLIKLQSGAFVEAVIMRYD+RLGKYG KP
Sbjct: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGAFVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEI+EQLVHASR  QIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240
           MGEPLNNYSALVEAVR M GSPFLLSPKRITISTVGIIHAI+KLHGDLPGLNLAVSLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKI IEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFQVVVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 360
           TFQVVVNLIPFNPIGSSSQF+TSSDEKVS FQ+VLRGTYNIRTT+RKQMGQDISGACGQL
Sbjct: 301 TFQVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQMGQDISGACGQL 360

Query: 361 VVTLPNRSSGKQTSVLPDIEDLHL 385
           VV+  NR SGKQT+VLPDIEDLHL
Sbjct: 361 VVSQTNRISGKQTNVLPDIEDLHL 384

BLAST of Lcy08g001360 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 548.5 bits (1412), Expect = 4.2e-156
Identity = 277/380 (72.89%), Postives = 318/380 (83.68%), Query Frame = 0

Query: 5   RSAFDAGEMRTEFEKAGIKSTFIPFIWKHLLHDPNNLFNNNLTSEFEWEQAVPSLPSVAY 64
           +S FDA E+++EFE AGI   F   IWK+++ +P+ +          W++ +PSLPS AY
Sbjct: 4   KSVFDASEIKSEFESAGINPKFAIQIWKYVIQNPDCV----------WDE-IPSLPSAAY 63

Query: 65  SLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGG 124
           SLL SKFK LTS++HS+  SSD  T+KLLIKLQ+GAFVEAV+MRYDTRLG  GGKPRPGG
Sbjct: 64  SLLHSKFKTLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGG 123

Query: 125 LRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRFSQIRNVVFMGMGEP 184
           +RSTLCISSQVGCKMGC FCATGTMGFKSNLTSGEI+EQLVHASR + IRN+VFMGMGEP
Sbjct: 124 IRSTLCISSQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIADIRNIVFMGMGEP 183

Query: 185 LNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQDI 244
           LNNY+A+VEAVR M   PF LSPKRITISTVGI+HAINKLH DLPG++LAVSLHAPVQ+I
Sbjct: 184 LNNYNAVVEAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPVQEI 243

Query: 245 RCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQV 304
           RCQIMPAARAFPLQKLMDALQ +Q+ SQQKI IEYIMLDGVND+EQHAH LG+LL+TFQV
Sbjct: 244 RCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKTFQV 303

Query: 305 VVNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQLVVTL 364
           V+NLIPFNPIGS+SQF TSS + VS FQK+LR TY IRTT+RK+MGQDISGACGQLVV  
Sbjct: 304 VINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISGACGQLVVNQ 363

Query: 365 PNRSSGKQTSVLPDIEDLHL 385
           P+      T  L DIEDL L
Sbjct: 364 PDIKKTPGTVELRDIEDLLL 372

BLAST of Lcy08g001360 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 5.2e-45
Identity = 115/299 (38.46%), Postives = 170/299 (56.86%), Query Frame = 0

Query: 66  LLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGGL 125
           L+   FK   S ++  V ++D  T KLL+KL+    +E V        G        G  
Sbjct: 115 LVDGGFKVGRSPIYQTVTATDG-TIKLLLKLEDNLLIETV--------GIPVQDDEKGIT 174

Query: 126 RSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRF--SQIRNVVFMGMGE 185
           R T C+SSQVGC + C FCATG  GF  NL   EI+EQ++        ++ NVVFMGMGE
Sbjct: 175 RLTACVSSQVGCPLRCSFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGE 234

Query: 186 PLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQD 245
           P+ N  ++++A R +      +  + ITISTVG+ + I KL        LAVSLHAP Q 
Sbjct: 235 PMLNLKSVLDAHRCL-NKDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQS 294

Query: 246 IRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQ 305
           +R +I+P+A+A+PL+ +M   ++Y +++ +++  EY +L GVND+ +HA +L +LL  + 
Sbjct: 295 LREKIVPSAKAYPLEAIMKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWG 354

Query: 306 VV--VNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 361
               VNLIP+NPI   S+++    + V  F   L  +  I  +VR+  G D S ACGQL
Sbjct: 355 KTYHVNLIPYNPI-EGSEYQRPYKKAVLAFAAALE-SRKITASVRQTRGLDASAACGQL 401

BLAST of Lcy08g001360 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 5.2e-45
Identity = 115/299 (38.46%), Postives = 170/299 (56.86%), Query Frame = 0

Query: 66  LLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGGL 125
           L+   FK   S ++  V ++D  T KLL+KL+    +E V        G        G  
Sbjct: 118 LVDGGFKVGRSPIYQTVTATDG-TIKLLLKLEDNLLIETV--------GIPVQDDEKGIT 177

Query: 126 RSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEILEQLVHASRF--SQIRNVVFMGMGE 185
           R T C+SSQVGC + C FCATG  GF  NL   EI+EQ++        ++ NVVFMGMGE
Sbjct: 178 RLTACVSSQVGCPLRCSFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGE 237

Query: 186 PLNNYSALVEAVRAMTGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQD 245
           P+ N  ++++A R +      +  + ITISTVG+ + I KL        LAVSLHAP Q 
Sbjct: 238 PMLNLKSVLDAHRCL-NKDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQS 297

Query: 246 IRCQIMPAARAFPLQKLMDALQEYQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQ 305
           +R +I+P+A+A+PL+ +M   ++Y +++ +++  EY +L GVND+ +HA +L +LL  + 
Sbjct: 298 LREKIVPSAKAYPLEAIMKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWG 357

Query: 306 VV--VNLIPFNPIGSSSQFRTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 361
               VNLIP+NPI   S+++    + V  F   L  +  I  +VR+  G D S ACGQL
Sbjct: 358 KTYHVNLIPYNPI-EGSEYQRPYKKAVLAFAAALE-SRKITASVRQTRGLDASAACGQL 404

BLAST of Lcy08g001360 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.8e-43
Identity = 120/340 (35.29%), Postives = 174/340 (51.18%), Query Frame = 0

Query: 30  IWKHLLHDPNNLFNNNLTSEFEWEQAVPSLPSVAYSLLR--SKFKPLTSTVHSVVDSSDQ 89
           +WK L  D  N++ NN          V  L  +   L R  S+     +     + S+  
Sbjct: 122 LWKRLYKD--NIWANN----------VDELEGLNKDLKRMISEHAEFGALSFKDIRSASD 181

Query: 90  VTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKPRPGGLRSTLCISSQVGCKMGCRFCATG 149
            T K+L  L  G  +E V++  D               R+T+C+SSQVGC M C+FC TG
Sbjct: 182 GTRKILFTLDDGLVIETVVIPCDRG-------------RTTVCVSSQVGCAMNCQFCYTG 241

Query: 150 TMGFKSNLTSGEILEQLVHASRF-----SQIRNVVFMGMGEPLNNYSALVEAVRAMTGSP 209
            MG K NLT+ EI+EQ V+A R        I NVVFMGMGEP +N   +++A   M    
Sbjct: 242 RMGLKRNLTTAEIVEQAVYARRLLSHEVGSITNVVFMGMGEPFHNIDNVIKAANIMVDEN 301

Query: 210 FL-LSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAPVQDIRCQIMPAARAFPLQKLM 269
            L  SP+++T+ST G++  + +   +     LAVSL+A   ++R  IMP  R + L  L+
Sbjct: 302 GLHFSPRKVTVSTSGLVPQLKRFLRE-SNCALAVSLNATTDEVRNWIMPINRKYKLSLLL 361

Query: 270 DALQE-YQRKSQQKILIEYIMLDGVNDEEQHAHQLGKLLETFQVVVNLIPFNPIGSSSQF 329
           + L+E    + + K+L EY+ML GVND    A +L +L++     +NLI FNP  S SQF
Sbjct: 362 ETLREGLSSRHKYKVLFEYVMLAGVNDSMDDARRLVELVQGIPCKINLIQFNP-HSGSQF 421

Query: 330 RTSSDEKVSIFQKVLRGTYNIRTTVRKQMGQDISGACGQL 361
             + ++K+  F+ VL         +R   G D   ACGQL
Sbjct: 422 IQTEEDKMIKFRNVL-AEGGCTVLMRFSRGNDQMAACGQL 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0LQM11.6e-5442.61Dual-specificity RNA methyltransferase RlmN OS=Syntrophobacter fumaroxidans (str... [more]
Q1DCU13.5e-5439.10Dual-specificity RNA methyltransferase RlmN 1 OS=Myxococcus xanthus (strain DK16... [more]
A9A3L94.5e-5439.24Ribosomal RNA large subunit methyltransferase N OS=Nitrosopumilus maritimus (str... [more]
Q6MDD02.9e-5341.32Probable dual-specificity RNA methyltransferase RlmN 2 OS=Protochlamydia amoebop... [more]
Q1MQJ39.5e-5239.88Dual-specificity RNA methyltransferase RlmN OS=Lawsonia intracellularis (strain ... [more]
Match NameE-valueIdentityDescription
A0A5D3C8Y42.9e-20593.23Dual-specificity RNA methyltransferase RlmN OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C1672.9e-20593.23dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103495... [more]
A0A6J1D5629.1e-19990.63uncharacterized protein LOC111017398 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1IBJ22.9e-19790.89uncharacterized protein LOC111470995 OS=Cucurbita maxima OX=3661 GN=LOC111470995... [more]
A0A6J1GM042.1e-19590.10uncharacterized protein LOC111455593 OS=Cucurbita moschata OX=3662 GN=LOC1114555... [more]
Match NameE-valueIdentityDescription
XP_038888929.14.5e-20895.05dual-specificity RNA methyltransferase RlmN [Benincasa hispida][more]
XP_008455036.16.1e-20593.23PREDICTED: dual-specificity RNA methyltransferase RlmN [Cucumis melo] >KAA003136... [more]
XP_011658875.17.9e-20592.97uncharacterized protein LOC101215180 [Cucumis sativus] >XP_031744517.1 uncharact... [more]
XP_022148839.11.9e-19890.63uncharacterized protein LOC111017398 [Momordica charantia][more]
XP_023553823.13.2e-19891.41uncharacterized protein LOC111811282 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G19630.14.2e-15672.89Radical SAM superfamily protein [more]
AT2G39670.15.2e-4538.46Radical SAM superfamily protein [more]
AT2G39670.25.2e-4538.46Radical SAM superfamily protein [more]
AT1G60230.14.8e-4335.29Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004383Ribosomal RNA large subunit methyltransferase RlmN/CfrPIRSFPIRSF006004Cfrcoord: 8..378
e-value: 4.4E-110
score: 365.7
IPR004383Ribosomal RNA large subunit methyltransferase RlmN/CfrSFLDSFLDF00275adenosine_C2_methyltransferascoord: 57..368
e-value: 0.0
score: 339.1
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 76..368
e-value: 9.5E-106
score: 355.1
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 132..290
e-value: 1.9E-13
score: 51.1
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 57..368
e-value: 0.0
score: 339.1
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 123..352
score: 30.592363
NoneNo IPR availablePFAMPF13394Fer4_14coord: 136..221
e-value: 1.2E-5
score: 25.4
NoneNo IPR availablePANTHERPTHR30544:SF8RADICAL SAM SUPERFAMILY PROTEINcoord: 3..378
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 136..337
e-value: 1.90527E-7
score: 49.2542
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 128..317
IPR040072Methyltransferase (Class A)PANTHERPTHR3054423S RRNA METHYLTRANSFERASEcoord: 3..378

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g001360.1Lcy08g001360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
biological_process GO:0030488 tRNA methylation
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding