Lcy08g000960 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g000960
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionL-galactose dehydrogenase
LocationChr08: 1409550 .. 1413105 (-)
RNA-Seq ExpressionLcy08g000960
SyntenyLcy08g000960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCCGCAAAAACGGATCTTGATCGATTGAATTGAGAGGCCCATCTGCTGCTACTTGGCGTTAATCTTCCAAAATCGGCATTATCCCTTCTCCACTTTCTTTCCCGGGAAATTCCATCTCTGTTCCAACTTCTGAGAGCTTCATCTTCACAATCAATGGCCGCTTCGTATCCCAAGCTCCAGTTGCGGAAGCTGGGAAACACCGGCCTCAAAACCAGCAGCGTCGGCTTCGGTGCCTCGCCTCTTGGCAGCGTCTTCGGCCCCGTTTCTGAGGACGATGCCGTCGCCTCCGTTCGTCAAGCCTTTCGTCTTGGCATCAACTTCTTCGACACCTCCCCGTGTGTAATCCCTTCTCTTTCTTTACTCGCACAATTTGCTTCCGGGAAATGGATTTATTTTGCGATTCGATCGTTACCCTTTTTGTGTTTTGGGAAGTAATAACCAATTCTTACCTCGAATCTGGAGTGAGTGTTCACCTGAAACTTGTCCAGTTTAACCTTTGCCTTGAACGAGTGTTGCAAGGTCAAAATTATTTTCCTGTGCGCTTAGTAAAAGAGGCTTGTTATGAGGTTGATGAGTTTTTAAAACAAACGATGCCTGGGGCTAGAGTTTTTAGGTTTTTTTCCATGATTTGTTTGGACTTTGATTTCGTGCATGCTTGATTTTCGTTGAGATTAGTTGGCTCTGGGAAGTTTTGCATTGCTTTTTGTTGAGGTTGGTTTTGGTAAGTGTCAGTTGCAACAAGGGTAAAAATTGTAACCTAAGAAGCATGGATATGGACACGAACACGAGACATTTATACGACACGATACTTAAAATCTGGGACACGGACATGACACTGACACTGACACGTCTAGTAAAATATACATTTTTTAAATATATATCATTTTTATACTAAGAAATTCAAAATCAAATAGTTTATGTATTTGTATGCTTAAGAAAATGAGTTTAATGTATTACATTCTTAAAATTATTATTTGGGTCATTTAGTATTGTGTCTATTTAGTATACTTAACAAGTGTTCGATATAGACCTATATTGACTTTGTACAACTAGTGTTTGTCACATATAGATGGTACTAATAAATGTCCGATACGTGTCCAATAAGTGTTAGAGTGTCCAAAACGCTAGCCAAACTAAAGTGTCCGTTCTTCTTAGATTGTAACATTTATCCAAGTTTGATGTCCAATTTAATTTGATGATATTGAAAGTTTAGGTGTTTGACTAACTTGCTTTCTAGTTTAACTCAGCATAAGGAAGAGCCAATGGCTTTGGGAAAATATATATTATTCTTTTTCATGTGTTTAACTCATGGTTAAGGATATATAAGGGCCTGCCTAGTCATCTTCAACATGGTCTCAATGGATCGATAATGTGTTTACTAATGCCTAGCTCTAGCACTTATATTTGGGCTTCTGTCCTCTCTACGAAGGTACTATGGAGCGACATTATCAGAAAAGATGCTTGGTAAGGGACTGAAAGCATTAGGAGTTCCAAGGAGTGAGTATATAGTCTCAACAAAGTGTGGGAGATACGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACAAGGAGCATTGATGAGAGTTTGGCTAGGCTACAACTAGATTACGTCGATATACTGCTCTGCCATGATATTGAATTTGGATCTCTTGATCAGGTCCGTGACTTGTCTGTTGATTTGTTACTTTCCTTAATAACTGATTGATCATTAACCTTTAGTTATCAACATGGTTATAGTTATACGACCTTACTTGAACAGGTTTCGCTTTATAGGTTGTAAAACTATGTCCTTGAGTTCTAAAAAGTTATTAACATGGTTATTGTCATTATCGTATTAAAGAAAAAGTGGATCTTCAGCTGCCTAAGTTTATTTCAGGTTGTGAATGAGACGATTCCTGCACTTCAAAAGCTGAAGGAAGCTGGGAAGACTCGTTTCATTGGTATTACAGGACTTCCATTGGAAATATTTACGTATGTGCTTGATCGAGTACCACCTGGGTCCATTGATGTGATTCTTTCATATTGTCACTACAGTATTAACGACTCAACATTGTTAGATTTGCTACCTTACTTGCAGAGTAAGGGAATTGGAATAATCAGTGCTTCTCCTCTAGCAATGGGACTTCTAACCAATTGTGGTCCCCCAGAATGGCACCCTGCTTCACCAGAATTGAAGGTACAGTTTCTCACGCCCTTTCTTTCATTAGCTTCAAAGTTTAATTGTGAGACTTAAAAAGCTTGAGCAGGGAAAGTGCTTTCAGTTGACTGATGTTGCATAAAGAAGACATTTGTTTTGTGGGTAGGGGGGGAGTTCATTTCTTGTTCTTTAAGCTGATGGGGTTTAAGGTCCCATTATGGAAATTTTATAGTATCCAGTAACTACTAGTTCGTTTGACCATACAAGCAAACTCCAAACCGGTTGTTTCTACTGCTGTGATTTTATTTTTTATTTTTCCAACTATGTTTTGTTTGAGCTTTGGCTTTTCATTGGAAAATGATAGTGTTCTGTTATTTGAAATTACTACAGTCTGCTTGTCGAGCTGCAGCTGCTCATTGTCAAAAGAAAGGAAAAAATATTTCGAAGTTAGCCCTCCAATACAGTCTGTCAAACAAAGCTATTTCAACAGTGCTGGTTGGCATGAACTCTGTCAGACAGGTATCTTTCAATAATCTCATTGTGTGGTATTCTTTTTCCGATATTACTTTCAATATCTTTGAGTCCAATCTCCTTTTCTGAAGATCAAGAATTGCCCCTGTGCCATCGTATATATACTAATTCTGTATCCATTAGTTTGTAGTTTCCTCTTCTATGCTTTTACTTCTGTGTGACTGACTACTTTATTGTCGAAAAAACTTGCCTCCACACTAGTTAGGAGGAAATCCTAATTTTCTGATATGGTTTATCTTTCGTGGATCAAAAAAAACTTCTTTTTGTAAAGATTACGGGTTCGATTGGTTCATTTTTAGTAGCAAACAGAACTTCTGATGCTGAAAATTTCAAGAGACTACATCTTAGAATCTTAACCAAGTTCTAACAATATGGAATCAGGTGGAGGAAAATGTAGCTGCTGCTGATGAACTTGCAACATTTGGGAGGGATGAGGAGACTCTATCAGAGGTTGAAGCTATCCTTGACCCTGTCAAGAATCAGACATGGCCAAGTGGAATCCAACAGAGCTGACCTTTTAGCTGCTGCTGCTGGTATGTGTAACATGTTCTTATGAATGTAGATCAACTACTGTATTTAACCTCATTCAAACTATCATGTGGGAGCTCCATCCATTTCTGTAGAAATTCGGTAGATAAGGCCGTGATCGTTTCACGACTCAGATGCACATCTAATTTTCCTTTATTCCCATTTCTCCCCCTCTTTGCAGCTTTGTTTTGTGTTTTAACAAAATAAATGATATGTGAAGTTAATCATGTCCTGGATATCACACCTGTGAAGTTGAGGGATACCTCATAGCTCTAGTAATGTGATTGATGTTTAGTAACAATTTGGTCCTGTTTGGTCAGCTGGAAAGAGTGGATAAATAAAGAATCGTGCTTTTTAA

mRNA sequence

ACCCGCAAAAACGGATCTTGATCGATTGAATTGAGAGGCCCATCTGCTGCTACTTGGCGTTAATCTTCCAAAATCGGCATTATCCCTTCTCCACTTTCTTTCCCGGGAAATTCCATCTCTGTTCCAACTTCTGAGAGCTTCATCTTCACAATCAATGGCCGCTTCGTATCCCAAGCTCCAGTTGCGGAAGCTGGGAAACACCGGCCTCAAAACCAGCAGCGTCGGCTTCGGTGCCTCGCCTCTTGGCAGCGTCTTCGGCCCCGTTTCTGAGGACGATGCCGTCGCCTCCGTTCGTCAAGCCTTTCGTCTTGGCATCAACTTCTTCGACACCTCCCCGTACTATGGAGCGACATTATCAGAAAAGATGCTTGGTAAGGGACTGAAAGCATTAGGAGTTCCAAGGAGTGAGTATATAGTCTCAACAAAGTGTGGGAGATACGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACAAGGAGCATTGATGAGAGTTTGGCTAGGCTACAACTAGATTACGTCGATATACTGCTCTGCCATGATATTGAATTTGGATCTCTTGATCAGGTTGTGAATGAGACGATTCCTGCACTTCAAAAGCTGAAGGAAGCTGGGAAGACTCGTTTCATTGGTATTACAGGACTTCCATTGGAAATATTTACGTATGTGCTTGATCGAGTACCACCTGGGTCCATTGATGTGATTCTTTCATATTGTCACTACAGTATTAACGACTCAACATTGTTAGATTTGCTACCTTACTTGCAGAGTAAGGGAATTGGAATAATCAGTGCTTCTCCTCTAGCAATGGGACTTCTAACCAATTGTGGTCCCCCAGAATGGCACCCTGCTTCACCAGAATTGAAGTCTGCTTGTCGAGCTGCAGCTGCTCATTGTCAAAAGAAAGGAAAAAATATTTCGAAGTTAGCCCTCCAATACAGTCTGTCAAACAAAGCTATTTCAACAGTGCTGGTTGGCATGAACTCTGTCAGACAGGTGGAGGAAAATGTAGCTGCTGCTGATGAACTTGCAACATTTGGGAGGGATGAGGAGACTCTATCAGAGGTTGAAGCTATCCTTGACCCTGTCAAGAATCAGACATGGCCAAGTGGAATCCAACAGAGCTGACCTTTTAGCTGCTGCTGCTGGTATGTGTAACATGTTCTTATGAATGTAGATCAACTACTGTATTTAACCTCATTCAAACTATCATGTGGGAGCTCCATCCATTTCTGTAGAAATTCGGTAGATAAGGCCGTGATCGTTTCACGACTCAGATGCACATCTAATTTTCCTTTATTCCCATTTCTCCCCCTCTTTGCAGCTTTGTTTTGTGTTTTAACAAAATAAATGATATGTGAAGTTAATCATGTCCTGGATATCACACCTGTGAAGTTGAGGGATACCTCATAGCTCTAGTAATGTGATTGATGTTTAGTAACAATTTGGTCCTGTTTGGTCAGCTGGAAAGAGTGGATAAATAAAGAATCGTGCTTTTTAA

Coding sequence (CDS)

ATGGCCGCTTCGTATCCCAAGCTCCAGTTGCGGAAGCTGGGAAACACCGGCCTCAAAACCAGCAGCGTCGGCTTCGGTGCCTCGCCTCTTGGCAGCGTCTTCGGCCCCGTTTCTGAGGACGATGCCGTCGCCTCCGTTCGTCAAGCCTTTCGTCTTGGCATCAACTTCTTCGACACCTCCCCGTACTATGGAGCGACATTATCAGAAAAGATGCTTGGTAAGGGACTGAAAGCATTAGGAGTTCCAAGGAGTGAGTATATAGTCTCAACAAAGTGTGGGAGATACGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACAAGGAGCATTGATGAGAGTTTGGCTAGGCTACAACTAGATTACGTCGATATACTGCTCTGCCATGATATTGAATTTGGATCTCTTGATCAGGTTGTGAATGAGACGATTCCTGCACTTCAAAAGCTGAAGGAAGCTGGGAAGACTCGTTTCATTGGTATTACAGGACTTCCATTGGAAATATTTACGTATGTGCTTGATCGAGTACCACCTGGGTCCATTGATGTGATTCTTTCATATTGTCACTACAGTATTAACGACTCAACATTGTTAGATTTGCTACCTTACTTGCAGAGTAAGGGAATTGGAATAATCAGTGCTTCTCCTCTAGCAATGGGACTTCTAACCAATTGTGGTCCCCCAGAATGGCACCCTGCTTCACCAGAATTGAAGTCTGCTTGTCGAGCTGCAGCTGCTCATTGTCAAAAGAAAGGAAAAAATATTTCGAAGTTAGCCCTCCAATACAGTCTGTCAAACAAAGCTATTTCAACAGTGCTGGTTGGCATGAACTCTGTCAGACAGGTGGAGGAAAATGTAGCTGCTGCTGATGAACTTGCAACATTTGGGAGGGATGAGGAGACTCTATCAGAGGTTGAAGCTATCCTTGACCCTGTCAAGAATCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

Protein sequence

MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSIDVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSACRAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEETLSEVEAILDPVKNQTWPSGIQQS
Homology
BLAST of Lcy08g000960 vs. ExPASy Swiss-Prot
Match: O81884 (L-galactose dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=LGALDH PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 1.7e-150
Identity = 263/317 (82.97%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 7   KLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGAT 66
           K++LR LGNTGLK S+VGFGASPLGSVFGPV+EDDAVA+VR+AFRLGINFFDTSPYYG T
Sbjct: 3   KIELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGT 62

Query: 67  LSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILL 126
           LSEKMLGKGLKAL VPRS+YIV+TKCGRY EGFDFSAERV +SIDESL RLQLDYVDIL 
Sbjct: 63  LSEKMLGKGLKALQVPRSDYIVATKCGRYKEGFDFSAERVRKSIDESLERLQLDYVDILH 122

Query: 127 CHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSIDVILSY 186
           CHDIEFGSLDQ+V+ETIPALQKLK+ GKTRFIGITGLPL+IFTYVLDRVPPG++DVILSY
Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182

Query: 187 CHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSACRAAAAH 246
           CHY +NDSTLLDLLPYL+SKG+G+ISASPLAMGLLT  GPPEWHPASPELKSA +AA AH
Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242

Query: 247 CQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEETLSEVEA 306
           C+ KGK I+KLALQYSL+NK IS+VLVGM+SV QVEENVAA  EL + G D+ETLSEVEA
Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302

Query: 307 ILDPVKNQTWPSGIQQS 324
           IL+PVKN TWPSGI Q+
Sbjct: 303 ILEPVKNLTWPSGIHQN 319

BLAST of Lcy08g000960 vs. ExPASy Swiss-Prot
Match: Q04212 (D-arabinose 1-dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ARA2 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-28
Identity = 89/292 (30.48%), Postives = 155/292 (53.08%), Query Frame = 0

Query: 46  VRQAFRLGINFFDTSPYYGATLSEKMLGKGLKAL--GVPRSEYIVSTKCGRY-AEGFDFS 105
           ++ AF  GIN  DTSPYYG   SE + G+ L  L    PR  Y + TK GR  AE F++S
Sbjct: 41  IKYAFSHGINAIDTSPYYGP--SEVLYGRALSNLRNEFPRDTYFICTKVGRIGAEEFNYS 100

Query: 106 AERVTRSIDESLARLQLDYVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITG 165
            + V  S+  S  RL   Y+D++  HD+EF     ++ E +  L+ LK  G  +  GI+G
Sbjct: 101 RDFVRFSVHRSCERLHTTYLDLVYLHDVEFVKFPDIL-EALKELRTLKNKGVIKNFGISG 160

Query: 166 LPLEIFTYVLDRVPP-----GSIDVILSYCHYSINDSTLLDLLPYL--QSKGIGIISASP 225
            P++  T++ +         GS+D +LSYC+ ++ ++ LL+    L   +K   + +AS 
Sbjct: 161 YPIDFITWLAEYCSTEESDIGSLDAVLSYCNLNLQNNKLLNFRERLLRNAKLKMVCNASI 220

Query: 226 LAMGLLTNCGPPEWHPASPELKSACRAAAAHCQKKGKNISKLALQYSLSN-KAISTVLVG 285
           L+M LL +    ++HP S EL+     AA +CQ++  +++ LA +Y++S       V++G
Sbjct: 221 LSMSLLRSQETRQFHPCSHELRECASQAAKYCQEQNVDLADLATRYAISEWVGKGPVVLG 280

Query: 286 MNSVRQVE-----ENVAAADELATFGRDEETLSEVEA-ILDPVKNQTWPSGI 321
           ++S+ +++       +  ++      +D + +  ++  I     N+ W SGI
Sbjct: 281 VSSMEELKLALDNYEIVKSNGNRLSSKDGQLVEYIQKNIFKEHFNEEWSSGI 329

BLAST of Lcy08g000960 vs. ExPASy Swiss-Prot
Match: Q52472 (D-threo-aldose 1-dehydrogenase OS=Pseudomonas sp. OX=306 GN=fdh PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.8e-27
Identity = 90/302 (29.80%), Postives = 143/302 (47.35%), Query Frame = 0

Query: 17  GLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGATLSEKMLGKGL 76
           GL   ++G+GA+ +G++F  +S+D+A A +  A+  GI ++DT+P+YG  LSEK LG  L
Sbjct: 12  GLAIPALGYGAANVGNLFRALSDDEAWAVLEAAWDAGIRYYDTAPHYGLGLSEKRLGAFL 71

Query: 77  KALGVPRSEYIVSTKCGR------------------------YAEGFDFSAERVTRSIDE 136
           +    PR E++VSTK GR                            +DF+ + +  SI E
Sbjct: 72  QT--KPRDEFVVSTKAGRLLRPNPERRPSGLDTDNDFHVPDDLRREWDFTEQGIRASIAE 131

Query: 137 SLARLQLDYVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVL 196
           S  RL LD +D+L  HD E   LD  +    PAL+K++  G  + IGI  +  +  T   
Sbjct: 132 SQERLGLDRIDLLYLHDPERHDLDLALASAFPALEKVRAEGVVKAIGIGSMVSDALTRA- 191

Query: 197 DRVPPGSIDVILSYCHYS-INDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPP---- 256
             V    +D+I+    Y+ +      ++LP       GI++AS    GLL    P     
Sbjct: 192 --VREADLDLIMVAGRYTLLEQPAATEVLPACAENATGIVAASVFNSGLLAQSEPKRDGR 251

Query: 257 -EWHPASPELKSACRAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVA 289
            E+     EL       AA C+     +   A+Q+ L +  + +V+VG +   Q+ +N  
Sbjct: 252 YEYGQLPDELWDRLVRIAAICRNHDVPLPAAAIQFPLQSALVRSVVVGGSRPAQLTQNAE 308

BLAST of Lcy08g000960 vs. ExPASy Swiss-Prot
Match: Q76KC2 (Pyridoxal 4-dehydrogenase OS=Microbacterium luteolum OX=69367 GN=pld1 PE=1 SV=3)

HSP 1 Score: 107.1 bits (266), Expect = 3.9e-22
Identity = 93/340 (27.35%), Postives = 149/340 (43.82%), Query Frame = 0

Query: 11  RKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGATLSEK 70
           R LG TGL  +++G G +PLG ++ PVS  DA A +   +  GI +FD++P YG    E 
Sbjct: 9   RALGRTGLTVTALGLGTAPLGGLYAPVSRADADALLEAGWDSGIRYFDSAPMYGYGRCEH 68

Query: 71  MLGKGLKALGVPRSEYIVSTKCGR-----------------------------YAEGFDF 130
           +LG  L+         ++STK GR                             + E FD+
Sbjct: 69  LLGDMLRE---KPERAVISTKVGRLMTNERAGRTLPPAPPKNPLDSGWHNGLNFREVFDY 128

Query: 131 SAERVTRSIDESLARLQLDYVDILLCHDI---------EFGSLDQVVNETIPALQKLKEA 190
           S + V RS D+S  RL    +D+L  HDI         EF            AL +L+ A
Sbjct: 129 SYDGVMRSFDDSQQRLGFPEIDLLYVHDIGRVTHADRHEFHWNALTRGGGFRALTELRAA 188

Query: 191 GKTRFIGITGLPLEIFTYVLDRVPPGSIDVILSYCHYSINDS-TLLDLLPYLQSKGIGII 250
           G  +     GL +  +  + D +    +D  L    YS+ D  +  + LP  Q +G+ ++
Sbjct: 189 GNIKGF---GLGVNEWQIIRDALEEADLDCSLLAGRYSLLDQVSEKEFLPLAQKRGMALV 248

Query: 251 SASPLAMGLLT--NCGPPEWHPASPELKSACRAAAAH--CQKKGKNISKLALQYSLSNKA 303
            A     G+L     G  ++  A    +   R    H  C +    ++  A+Q+ L ++A
Sbjct: 249 IAGVFNSGILAAPRGGEQKFDYADAPAEIIARTNRLHDICDEYHVPLAAAAMQFPLRHEA 308

BLAST of Lcy08g000960 vs. ExPASy Swiss-Prot
Match: Q8X529 (L-glyceraldehyde 3-phosphate reductase OS=Escherichia coli O157:H7 OX=83334 GN=gpr PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 8.6e-22
Identity = 97/327 (29.66%), Postives = 165/327 (50.46%), Query Frame = 0

Query: 5   YPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSE-DDAVASVRQAFRLGINFFDTSPYY 64
           Y ++Q R  G +GL+  ++  G   L   FG V+  +   A +R+AF LGI  FD +  Y
Sbjct: 10  YGQMQYRYCGKSGLRLPALSLG---LWHNFGHVNALESQRAILRKAFDLGITHFDLANNY 69

Query: 65  GAT--LSEKMLGKGLKA-LGVPRSEYIVSTKC------GRYAEGFDFSAERVTRSIDESL 124
           G     +E+  G+ L+      R E I+STK       G Y  G   S + +  S+D+SL
Sbjct: 70  GPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDMWPGPYGSG--GSRKYLLASLDQSL 129

Query: 125 ARLQLDYVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDR 184
            R+ L+YVDI   H ++  +    + ET  AL    ++GK  ++GI+    E    +++ 
Sbjct: 130 KRMGLEYVDIFYSHRVDENT---PMEETASALAHAVQSGKALYVGISSYSPERTQKMVEL 189

Query: 185 VPPGSIDVILSYCHYS-----INDSTLLDLLPYLQSKGIGIISASPLAMGLLTN---CGP 244
           +    I +++    Y+     ++ S LLD    LQ+ G+G I+ +PLA GLLT     G 
Sbjct: 190 LHEWKIPLLIHQPSYNLLNRWVDKSGLLDT---LQNNGVGCIAFTPLAQGLLTGKYLNGI 249

Query: 245 PE---WHPASPELK------------SACRAAAAHCQKKGKNISKLALQYSLSNKAISTV 299
           PE    H    +++            ++ R      Q++G++++++AL + L ++ +++V
Sbjct: 250 PEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQRGQSMAQMALSWLLKDERVTSV 309

BLAST of Lcy08g000960 vs. ExPASy TrEMBL
Match: A0A6J1GJU5 (L-galactose dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111455013 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.1e-168
Identity = 306/323 (94.74%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKAL VPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQKL+EAGK RFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SK IGIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGK+ISKLALQYSLSNK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. ExPASy TrEMBL
Match: A0A6J1IA20 (L-galactose dehydrogenase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471025 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.4e-168
Identity = 307/323 (95.05%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFG VSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGLVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKAL VPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SK IGIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGK+ISKLALQYSLSNK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. ExPASy TrEMBL
Match: A0A6J1DMP0 (L-galactose dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111022462 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 300/323 (92.88%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS
Sbjct: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKALGVPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQ LKE+GK  FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYL+SKGIG+I+ASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGKNISKLALQYSLSNK ISTVLVGMNSVRQVE+NVAAA+ELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL+PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILEPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. ExPASy TrEMBL
Match: A0A1S3C0W3 (L-galactose dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103495364 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.7e-164
Identity = 294/323 (91.02%), Postives = 311/323 (96.28%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MA SYPKL LR+LGNTGLKTSSVGFGASPLGSVF PVSE+DAVA+VR+AFRLGINFFDTS
Sbjct: 1   MAVSYPKLPLRELGNTGLKTSSVGFGASPLGSVFSPVSEEDAVAAVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG  LSEKMLGKGLKALGVPRSEYIVSTKCGRY EGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGRNLSEKMLGKGLKALGVPRSEYIVSTKCGRYGEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQ+VNETIPALQKLKEAGKTRF+GITGLPLEIFTYVLDRVPPG+I
Sbjct: 121 YVDILHCHDIEFGSLDQIVNETIPALQKLKEAGKTRFLGITGLPLEIFTYVLDRVPPGTI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SKG+GIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKGVGIISASPLAMGLLTDQGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAA HC+KKG+NI+KLA+QYSL NK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 QAAADHCRKKGRNITKLAIQYSLVNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL+PVKNQTWPSGIQ S
Sbjct: 301 LSEVEAILNPVKNQTWPSGIQNS 323

BLAST of Lcy08g000960 vs. ExPASy TrEMBL
Match: A0A5A7SPF2 (L-galactose dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001760 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.7e-164
Identity = 294/323 (91.02%), Postives = 311/323 (96.28%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MA SYPKL LR+LGNTGLKTSSVGFGASPLGSVF PVSE+DAVA+VR+AFRLGINFFDTS
Sbjct: 1   MAVSYPKLPLRELGNTGLKTSSVGFGASPLGSVFSPVSEEDAVAAVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG  LSEKMLGKGLKALGVPRSEYIVSTKCGRY EGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGRNLSEKMLGKGLKALGVPRSEYIVSTKCGRYGEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQ+VNETIPALQKLKEAGKTRF+GITGLPLEIFTYVLDRVPPG+I
Sbjct: 121 YVDILHCHDIEFGSLDQIVNETIPALQKLKEAGKTRFLGITGLPLEIFTYVLDRVPPGTI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SKG+GIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKGVGIISASPLAMGLLTDQGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAA HC+KKG+NI+KLA+QYSL NK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 QAAADHCRKKGRNITKLAIQYSLVNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL+PVKNQTWPSGIQ S
Sbjct: 301 LSEVEAILNPVKNQTWPSGIQNS 323

BLAST of Lcy08g000960 vs. NCBI nr
Match: KAG7011583.1 (L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.5 bits (1547), Expect = 8.5e-168
Identity = 306/323 (94.74%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 47  MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 106

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKAL VPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 107 PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 166

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQKL+EAGK RFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 167 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 226

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SK IGIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 227 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 286

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGK+ISKLALQYSLSNK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 287 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 346

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 347 LSEVEAILHPVKNQTWPSGIQQS 369

BLAST of Lcy08g000960 vs. NCBI nr
Match: XP_022952291.1 (L-galactose dehydrogenase [Cucurbita moschata])

HSP 1 Score: 600.5 bits (1547), Expect = 8.5e-168
Identity = 306/323 (94.74%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKAL VPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQKL+EAGK RFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SK IGIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGK+ISKLALQYSLSNK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. NCBI nr
Match: XP_022972473.1 (L-galactose dehydrogenase isoform X1 [Cucurbita maxima])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 307/323 (95.05%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFG VSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGLVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKAL VPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SK IGIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGK+ISKLALQYSLSNK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. NCBI nr
Match: KAG6571895.1 (L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 599.4 bits (1544), Expect = 1.9e-167
Identity = 306/323 (94.74%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKAL VPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQKL+EAGK RFIGITGLPLEIFTYVLDRVPPGSI
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLLPYL+SK IGIISASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
            AAAAHC+KKGK+ISKLALQYSLSNK ISTVLVGMNSVRQVEENVAAA+ELATFGRDEET
Sbjct: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. NCBI nr
Match: XP_022155328.1 (L-galactose dehydrogenase [Momordica charantia])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 300/323 (92.88%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS
Sbjct: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60

Query: 61  PYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG TLSEKMLGKGLKALGVPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180
           YVDIL CHDIEFGSLDQVVNETIPALQ LKE+GK  FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180

Query: 181 DVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYL+SKGIG+I+ASPLAMGLLT+ GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240

Query: 241 RAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEET 300
           +AAAAHC+KKGKNISKLALQYSLSNK ISTVLVGMNSVRQVE+NVAAA+ELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300

Query: 301 LSEVEAILDPVKNQTWPSGIQQS 324
           LSEVEAIL+PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILEPVKNQTWPSGIQQS 323

BLAST of Lcy08g000960 vs. TAIR 10
Match: AT4G33670.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.2e-151
Identity = 263/317 (82.97%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 7   KLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGAT 66
           K++LR LGNTGLK S+VGFGASPLGSVFGPV+EDDAVA+VR+AFRLGINFFDTSPYYG T
Sbjct: 3   KIELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGT 62

Query: 67  LSEKMLGKGLKALGVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILL 126
           LSEKMLGKGLKAL VPRS+YIV+TKCGRY EGFDFSAERV +SIDESL RLQLDYVDIL 
Sbjct: 63  LSEKMLGKGLKALQVPRSDYIVATKCGRYKEGFDFSAERVRKSIDESLERLQLDYVDILH 122

Query: 127 CHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSIDVILSY 186
           CHDIEFGSLDQ+V+ETIPALQKLK+ GKTRFIGITGLPL+IFTYVLDRVPPG++DVILSY
Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182

Query: 187 CHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELKSACRAAAAH 246
           CHY +NDSTLLDLLPYL+SKG+G+ISASPLAMGLLT  GPPEWHPASPELKSA +AA AH
Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242

Query: 247 CQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELATFGRDEETLSEVEA 306
           C+ KGK I+KLALQYSL+NK IS+VLVGM+SV QVEENVAA  EL + G D+ETLSEVEA
Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302

Query: 307 ILDPVKNQTWPSGIQQS 324
           IL+PVKN TWPSGI Q+
Sbjct: 303 ILEPVKNLTWPSGIHQN 319

BLAST of Lcy08g000960 vs. TAIR 10
Match: AT1G60750.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 92/317 (29.02%), Postives = 144/317 (45.43%), Query Frame = 0

Query: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFG-PVSEDDAVASVRQAFRLGINFFDT 60
           MA    +++  KLG+ GL+ S+ G G   L   +G P  E +AVA +R A   G+ F DT
Sbjct: 1   MAEEACQVRRMKLGSQGLEVSAQGLGCMGLSDFYGAPTPETNAVALLRHAINAGVTFLDT 60

Query: 61  SPYYGATLSEKMLGKGLKALGVPRSEYIVSTKCGRYAE-----GFDFSAERVTRSIDESL 120
           S  YG   +E +LGK LK  G+ R +  ++TK G  A      GF    E V  + + SL
Sbjct: 61  SDIYGPETNELLLGKALKD-GL-RDKVELATKFGITASEDGKFGFRGDPEYVRIACEASL 120

Query: 121 ARLQLDYVDILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDR 180
            RL +  +D+   H I+       +  TI  L+KL E GK ++IG++             
Sbjct: 121 KRLGVTCIDLYYQHRID---TTLPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAV 180

Query: 181 VPPGSIDVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASP 240
            P  ++ +  S     + +    D++P  +  GIGI++ SPL  G L   G P +   + 
Sbjct: 181 HPITAVQIEWSLWSRDVEE----DIIPTCRELGIGIVAYSPLGRGFL---GLPRFQQENL 240

Query: 241 E-LKSACRAAAAHCQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAADELAT 300
           E  K       A   KK    ++LAL +          + G + ++ + +N+ A     T
Sbjct: 241 ENNKILYEKVQAMATKKSCTPAQLALAWVHHQGDDVCPIPGTSKIQNLNQNIGALSVKLT 300

Query: 301 FGRDEETLSEVEAILDP 311
                E + E+EAI  P
Sbjct: 301 ----PEEMVELEAIAQP 301

BLAST of Lcy08g000960 vs. TAIR 10
Match: AT1G04690.1 (potassium channel beta subunit 1 )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 85/311 (27.33%), Postives = 141/311 (45.34%), Query Frame = 0

Query: 8   LQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFR-LGINFFDTSPYYGAT 67
           +Q + LG +GLK S++ FGA      FG   +     S+ Q  R  G+NFFD +  Y   
Sbjct: 1   MQYKNLGKSGLKVSTLSFGA---WVTFGNQLDVKEAKSILQCCRDHGVNFFDNAEVYANG 60

Query: 68  LSEKMLGKGLKALGVPRSEYIVSTKC---GRYAEGFDFSAERVTRSIDESLARLQLDYVD 127
            +E+++G+ ++ LG  RS+ ++STK    G        S + +      SL RL +DYVD
Sbjct: 61  RAEEIMGQAIRELGWRRSDIVISTKIFWGGPGPNDKGLSRKHIVEGTKASLKRLDMDYVD 120

Query: 128 ILLCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYV------LDRVPP 187
           +L CH  +  +    + ET+ A+  + + G   + G +    +  T        LD V P
Sbjct: 121 VLYCHRPDAST---PIEETVRAMNYVIDKGWAFYWGTSEWSAQQITEAWGAADRLDLVGP 180

Query: 188 GSIDVILSYCHYSI--NDSTLLDLLPYLQSKGIGIISASPLAMGLLT---NCG--PPEWH 247
                I+    Y++        + LP   + GIG+ + SPLA G+LT   N G  P +  
Sbjct: 181 -----IVEQPEYNMFARHKVETEFLPLYTNHGIGLTTWSPLASGVLTGKYNKGAIPSDSR 240

Query: 248 PASPELKSACRAAAAH------------CQKKGKNISKLALQYSLSNKAISTVLVGMNSV 290
            A    K+    +                 + G  +++LA+ +  SN  +S+V+ G    
Sbjct: 241 FALENYKNLANRSLVDDVLRKVSGLKPIADELGVTLAQLAIAWCASNPNVSSVITGATRE 300

BLAST of Lcy08g000960 vs. TAIR 10
Match: AT2G37760.2 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 4.3e-08
Identity = 75/293 (25.60%), Postives = 122/293 (41.64%), Query Frame = 0

Query: 15  NTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGATLSEKMLGK 74
           NTG K   VG G   +              ++ QA ++G    D +  YG   +EK +G 
Sbjct: 11  NTGAKLPCVGLGTYAM-----------VATAIEQAIKIGYRHIDCASIYG---NEKEIGG 70

Query: 75  GLKAL----GVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILLCH-- 134
            LK L     V R E  +++K        D   E V ++++++L  LQ+DYVD+ L H  
Sbjct: 71  VLKKLIGDGFVKREELFITSKLW----SNDHLPEDVPKALEKTLQDLQIDYVDLYLIHWP 130

Query: 135 -DIEFGSLDQV--------VNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLD--RVPP 194
             ++  SL           +  T  A++ L ++GK R IG++    +  T +L+  RV P
Sbjct: 131 ASLKKESLMPTPEMLTKPDITSTWKAMEALYDSGKARAIGVSNFSSKKLTDLLNVARVTP 190

Query: 195 GSIDVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELK 254
               V    CH       L +L    +SKG+ +   SPL                   LK
Sbjct: 191 AVNQV---ECHPVWQQQGLHEL---CKSKGVHLSGYSPLG----------SQSKGEVRLK 250

Query: 255 SACRAAAAH-CQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAAD 290
                      +K GK  +++AL++ L      +VL   +S  +++EN+   D
Sbjct: 251 VLQNPIVTEVAEKLGKTTAQVALRWGLQTG--HSVLPKSSSGARLKENLDVFD 267

BLAST of Lcy08g000960 vs. TAIR 10
Match: AT2G37760.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 4.3e-08
Identity = 75/293 (25.60%), Postives = 122/293 (41.64%), Query Frame = 0

Query: 15  NTGLKTSSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGATLSEKMLGK 74
           NTG K   VG G   +              ++ QA ++G    D +  YG   +EK +G 
Sbjct: 11  NTGAKLPCVGLGTYAM-----------VATAIEQAIKIGYRHIDCASIYG---NEKEIGG 70

Query: 75  GLKAL----GVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILLCH-- 134
            LK L     V R E  +++K        D   E V ++++++L  LQ+DYVD+ L H  
Sbjct: 71  VLKKLIGDGFVKREELFITSKLW----SNDHLPEDVPKALEKTLQDLQIDYVDLYLIHWP 130

Query: 135 -DIEFGSLDQV--------VNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLD--RVPP 194
             ++  SL           +  T  A++ L ++GK R IG++    +  T +L+  RV P
Sbjct: 131 ASLKKESLMPTPEMLTKPDITSTWKAMEALYDSGKARAIGVSNFSSKKLTDLLNVARVTP 190

Query: 195 GSIDVILSYCHYSINDSTLLDLLPYLQSKGIGIISASPLAMGLLTNCGPPEWHPASPELK 254
               V    CH       L +L    +SKG+ +   SPL                   LK
Sbjct: 191 AVNQV---ECHPVWQQQGLHEL---CKSKGVHLSGYSPLG----------SQSKGEVRLK 250

Query: 255 SACRAAAAH-CQKKGKNISKLALQYSLSNKAISTVLVGMNSVRQVEENVAAAD 290
                      +K GK  +++AL++ L      +VL   +S  +++EN+   D
Sbjct: 251 VLQNPIVTEVAEKLGKTTAQVALRWGLQTG--HSVLPKSSSGARLKENLDVFD 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818841.7e-15082.97L-galactose dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=LGALDH PE=1 SV=1[more]
Q042121.2e-2830.48D-arabinose 1-dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Q524726.8e-2729.80D-threo-aldose 1-dehydrogenase OS=Pseudomonas sp. OX=306 GN=fdh PE=1 SV=1[more]
Q76KC23.9e-2227.35Pyridoxal 4-dehydrogenase OS=Microbacterium luteolum OX=69367 GN=pld1 PE=1 SV=3[more]
Q8X5298.6e-2229.66L-glyceraldehyde 3-phosphate reductase OS=Escherichia coli O157:H7 OX=83334 GN=g... [more]
Match NameE-valueIdentityDescription
A0A6J1GJU54.1e-16894.74L-galactose dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111455013 PE=4 SV=... [more]
A0A6J1IA205.4e-16895.05L-galactose dehydrogenase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471025... [more]
A0A6J1DMP01.6e-16792.88L-galactose dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111022462 PE=4 SV... [more]
A0A1S3C0W34.7e-16491.02L-galactose dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103495364 PE=4 SV=1[more]
A0A5A7SPF24.7e-16491.02L-galactose dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
KAG7011583.18.5e-16894.74L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022952291.18.5e-16894.74L-galactose dehydrogenase [Cucurbita moschata][more]
XP_022972473.11.1e-16795.05L-galactose dehydrogenase isoform X1 [Cucurbita maxima][more]
KAG6571895.11.9e-16794.74L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022155328.13.2e-16792.88L-galactose dehydrogenase [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G33670.11.2e-15182.97NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60750.11.1e-1929.02NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G04690.11.1e-1927.33potassium channel beta subunit 1 [more]
AT2G37760.24.3e-0825.60NAD(P)-linked oxidoreductase superfamily protein [more]
AT2G37760.14.3e-0825.60NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 8..319
e-value: 2.9E-75
score: 255.2
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 8..308
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 23..290
e-value: 3.2E-57
score: 193.9
NoneNo IPR availablePIRSRPIRSR000097-2PIRSR000097-2coord: 19..218
e-value: 6.6E-18
score: 62.5
NoneNo IPR availablePIRSRPIRSR000097-1PIRSR000097-1coord: 19..218
e-value: 6.6E-18
score: 62.5
NoneNo IPR availablePIRSRPIRSR000097-3PIRSR000097-3coord: 19..218
e-value: 6.6E-18
score: 62.5
IPR020471Aldo-keto reductasePANTHERPTHR42686GH17980P-RELATEDcoord: 7..320
IPR044479L-galactose dehydrogenase-likeCDDcd19163AKR_galDHcoord: 11..290
e-value: 4.53519E-178
score: 492.066

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g000960.1Lcy08g000960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity
molecular_function GO:0010349 L-galactose dehydrogenase activity
molecular_function GO:0050235 pyridoxal 4-dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity